CN104823824A - Temporary immersion type culture method and culture device for dendrobe - Google Patents
Temporary immersion type culture method and culture device for dendrobe Download PDFInfo
- Publication number
- CN104823824A CN104823824A CN201510225344.7A CN201510225344A CN104823824A CN 104823824 A CN104823824 A CN 104823824A CN 201510225344 A CN201510225344 A CN 201510225344A CN 104823824 A CN104823824 A CN 104823824A
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- stem
- noble dendrobium
- epicoele
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- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G2031/006—Soilless cultivation, e.g. hydroponics with means for recycling the nutritive solution
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
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- Pretreatment Of Seeds And Plants (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a temporary immersion type culture method and culture device for dendrobe. Rooted sterile dendrobe seedlings are transferred to a temporary immersion type culture device and are subjected to liquid culture under a natural condition, no sugars are added into culture solution, no carbon dioxide needs to be introduced, and carbon sources required for the growth of the dendrobe are from self photosynthesis. According to the method, a dendrobe culture condition is simplified, and a plantation cost is lowered.
Description
Technical field
The present invention relates to biotechnology, particularly a kind of intermittent immersed cultural method of the stem of noble dendrobium and culture apparatus.
Technical background
The stem of noble dendrobium is the nourishing class Chinese herbal medicine precious simply of China, has tonifying Qi stomach invigorating, nourishing kidney yin, strengthens the effect of body immunity, be classified as top grade in Shennong's Herbal.Because consumption is huge, be faced with two crises at present: one is that wild plant resource reserves and output ubiquity downward trend; Two is that stem of noble dendrobium demand constantly increases, and causes and excavates the excess of this resources of medicinal plant.Traditional dendrobe cultivation technology mainly comprises live tree inoculated and cultured and soilless substyate culture.Tree inoculated and cultured of living output is too low; When soilless substrate is cultivated, matrix used is based on pine bark, not only destroys valuable tree resources, and adds planting cost.By finding the retrieval of prior art, Jiang Ping etc. (2013) have invented a kind of " a kind of sugar-free tissue culture method of dendrobium officinale ", Xiao Yulan etc. (2012) have invented " a kind of cultural method of saccharide-free box-type plant tissue ", Wang Xilin etc. (2006) have invented a kind of " Aeroponic type plant sugar-free tissue culturing device ", these patents of invention can reduce the pollution rate of the aseptic plantlet in vitro of the stem of noble dendrobium, but when growing under not solving the outer natural conditions of stem of noble dendrobium seedling room, the problem of required matrix.In addition, the ventilation requirement of the stem of noble dendrobium to root is high, and in above-mentioned patent, plant long period of soaking in a liquid, easily causes rotten; At the bottom of case, pass into carbonic acid gas in addition can increase planting cost.
Summary of the invention
Object of the present invention, exactly in order to overcome above-mentioned prior art Problems existing, provides a kind of intermittent immersed cultural method and culture apparatus of the stem of noble dendrobium.
In order to realize object of the present invention, present invention employs following technical scheme: a kind of intermittent immersed cultural method of the stem of noble dendrobium, comprises the steps:
One, with mercuric chloride solution, stem of noble dendrobium seed is carried out disinfection, be then inoculated in seed germination medium, cultivate under illumination;
Two, aseptic seedling opens bottle cap after taking root, and practices seedling 2-3 days;
Three, practice seedling and terminate the residual agar of afterwash root, then proceed in intermittent immersed culture apparatus;
Four, inject nutrient solution at intermittent immersed culture apparatus cavity of resorption, then aseptic for stem of noble dendrobium seedling cuttage auto levelizer epicoele is also fixed with the netted seedbed of stainless steel; Adopt intermittent immersed training mode to cultivate stem of noble dendrobium seedling, allow nutrient solution intermittent cyclic transfer between the epicoele and cavity of resorption of device under the acting in conjunction of siphon pipe and liquid pump.
Seed germination medium described in step one, for adding the MS solid culture medium of 0.2-0.5mg/L gibberellin.
Described in step 4, intermittent immersed training mode is, at room temperature, intermittent immersed culture apparatus is placed in outdoor shady and cool place, avoid direct sunlight, do not need gnotobasis, the periodic Control of nutrient solution intermittent cyclic transfer is at 30-60 minute, and every 15-20 days changes a fresh nutrient solution.
The formula of described nutrient solution is the 1/2MS sugar-free culture-medium that macroelement reduces by half, and stem of noble dendrobium growth desired sugars part is from photosynthesis.
For the culture apparatus of the intermittent immersed cultural method of the above-mentioned stem of noble dendrobium, comprise incubator, this incubator becomes epicoele and cavity of resorption by one piece of baffle for separating, between epicoele and cavity of resorption, be connected to communicating pipe, siphon pipe and liquid pump, be also provided with the netted seedbed of stainless steel for fixing stem of noble dendrobium seedling at epicoele.
Described siphonal siphon port is close at the bottom of the chamber of epicoele.
Adopt apparatus and method of the present invention, by the sterile dendrobe seedling of having taken root, transfer in an intermittent immersed culture apparatus, carry out liquid culture under field conditions (factors), not sugaring in culture fluid, does not need to pass into carbonic acid gas yet, and the carbon source needed for stem of noble dendrobium growth comes from self photosynthesis, simplify condition of culture, reduce planting cost.
Accompanying drawing explanation
Fig. 1 is the structural representation of the intermittent immersed culture apparatus of the stem of noble dendrobium.
Specific implementation method
The intermittent immersed cultural method of the stem of noble dendrobium of the present invention, comprises the steps:
One, with mercuric chloride solution, stem of noble dendrobium seed is carried out disinfection, be then inoculated in seed germination medium, cultivate under indoor illumination; Seed germination medium is the MS solid culture medium adding 0.5mg/L gibberellin.
Two, aseptic seedling opens bottle cap after taking root, and practices seedling 2 days;
Three, practice seedling and terminate the residual agar of afterwash root, then proceed in intermittent immersed culture apparatus and cultivate.
Four, at room temperature, intermittent immersed culture apparatus is placed in outdoor shady and cool place, avoids direct sunlight.Inject nutrient solution at intermittent immersed culture apparatus cavity of resorption, the formula of nutrient solution is the 1/2MS sugar-free culture-medium that macroelement reduces by half, and then aseptic for stem of noble dendrobium seedling cuttage auto levelizer epicoele is also fixed with the netted seedbed 5 of stainless steel; Open liquid pump 3, nutrient solution transfers to epicoele from the cavity of resorption of container under the effect of liquid pump, when the liquid level of epicoele liquid exceedes the peak of siphon pipe 4, (peak of siphon pipe 4 is higher than the peak of stem of noble dendrobium seedling, ensure all stem of noble dendrobium seedlings of nutrient solution energy submergence), the culture fluid of container epicoele is transferred to rapidly below container under siphonage.So, nutrient solution is reciprocation cycle transfer between the upper and lower chamber of container, and stem of noble dendrobium seedling realizes intermittent immersed cultivation under will being in soaked with liquid and the exposed condition alternately occurred.The periodic Control of nutrient solution intermittent cyclic transfer is at 30-60 minute, and every 15-20 days changes a fresh nutrient solution.
The present embodiment culture apparatus used as shown in Figure 1, comprise incubator 1, this incubator becomes epicoele 11 and cavity of resorption 12 by one piece of baffle for separating, is connected to communicating pipe 2, siphon pipe 4 and liquid pump 3 between epicoele and cavity of resorption, is also provided with the stainless (steel) wire 5 for cuttage stem of noble dendrobium seedling at epicoele.Siphonal siphon port is close at the bottom of the chamber of epicoele.
Incubator is placed in the greenhouse being provided with shading screen, and temperature is about 20 degree, and illumination is natural daylight.Culture fluid is the 1/2MS sugar-free culture-medium that macroelement reduces by half, and the resting period is 30 minutes, and displacement in the every 15-20 of nutrient solution days once.After 30 days, statistics stone solution biomass, result growth is slightly slower than traditional solid matrix training mode, but cost obviously reduces.
In the present embodiment, stem of noble dendrobium seedling used is from this laboratory, and culture apparatus used and Shanghai calm and peaceful glass apparatus factory is customized, and liquor pump is provided by Baoding Beng Ye Co., Ltd.
Claims (6)
1. an intermittent immersed cultural method for the stem of noble dendrobium, is characterized in that: comprise the steps:
One, with mercuric chloride solution, stem of noble dendrobium seed is carried out disinfection, be then inoculated in seed germination medium, cultivate under illumination;
Two, aseptic seedling opens bottle cap after taking root, and practices seedling 2-3 days;
Three, practice seedling and terminate the residual agar of afterwash root, then proceed in intermittent immersed culture apparatus;
Four, inject nutrient solution at intermittent immersed culture apparatus cavity of resorption, then aseptic for stem of noble dendrobium seedling cuttage auto levelizer epicoele is also fixed with the netted seedbed of stainless steel; Adopt intermittent immersed training mode to cultivate stem of noble dendrobium seedling, allow nutrient solution intermittent cyclic transfer between the epicoele and cavity of resorption of device under the acting in conjunction of siphon pipe and liquid pump.
2. cultural method according to claim 1, is characterized in that, seed germination medium described in step one, for adding the MS solid culture medium of 0.2-0.5mg/L gibberellin.
3. cultural method according to claim 1, it is characterized in that, described in step 4, intermittent immersed training mode is, at room temperature, intermittent immersed culture apparatus is placed in outdoor shady and cool place, avoids direct sunlight, do not need gnotobasis, the periodic Control of nutrient solution intermittent cyclic transfer is at 30-60 minute, and every 15-20 days changes a fresh nutrient solution.
4. cultural method according to claim 1, is characterized in that, the formula of described nutrient solution is the 1/2MS sugar-free culture-medium that macroelement reduces by half, and stem of noble dendrobium growth desired sugars part is from photosynthesis.
5. for the culture apparatus of the intermittent immersed cultural method of the stem of noble dendrobium described in claim 1, it is characterized in that: comprise incubator, this incubator becomes epicoele and cavity of resorption by one piece of baffle for separating, between epicoele and cavity of resorption, be connected to communicating pipe, siphon pipe and liquid pump, be also provided with the netted seedbed of stainless steel for fixing stem of noble dendrobium seedling at epicoele.
6. the intermittent immersed culture apparatus of the stem of noble dendrobium according to claim 5, it is characterized in that, described siphonal siphon port is close at the bottom of the chamber of epicoele.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191798A (en) * | 2015-09-29 | 2015-12-30 | 中国科学院植物研究所 | Temporary-immersion lily scale rapid-propagation method |
| CN106069342A (en) * | 2016-07-01 | 2016-11-09 | 新疆维吾尔自治区水土保持生态环境监测总站 | A kind of Populus euphratica seedling cultural method and device thereof |
| CN106172009A (en) * | 2016-08-15 | 2016-12-07 | 上海离草科技有限公司 | Plant sugar-free culture systems and plant cultivation container |
| CN107318646A (en) * | 2017-05-22 | 2017-11-07 | 江苏绿洲园艺绿化有限公司 | One kind comb lip stem of noble dendrobium seedling fast breeding method |
| CN107896964A (en) * | 2017-10-30 | 2018-04-13 | 宁波神乙草生物科技有限公司 | Dendrobium candidum breeding apparatus |
| CN109169242A (en) * | 2018-09-30 | 2019-01-11 | 河北科技师范学院 | A kind of water culture basin alms bowl, column and culture apparatus |
| CN109644617A (en) * | 2019-01-09 | 2019-04-19 | 贵州省旱粮研究所 | A kind of layering culture dish and its transfer tool handgrip |
| CN111771708A (en) * | 2020-07-14 | 2020-10-16 | 深圳市航天食品分析测试中心有限公司 | Indoor dendrobium nobile soilless culture device and method thereof |
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| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
| CN103039368A (en) * | 2013-01-27 | 2013-04-17 | 云南天泉生物科技股份有限公司 | Sugar-free tissue culture method of dendrobium officinale |
| CN103270946A (en) * | 2013-02-04 | 2013-09-04 | 南京博方生物科技有限公司 | Dendrobium officinale seedling production and hardening synchronization method |
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Patent Citations (4)
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| CN1188593A (en) * | 1997-01-22 | 1998-07-29 | 中国科学院遗传研究所 | Culture container and method for producing potato miniature potato etc. plant material using container thereof |
| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191798A (en) * | 2015-09-29 | 2015-12-30 | 中国科学院植物研究所 | Temporary-immersion lily scale rapid-propagation method |
| CN105191798B (en) * | 2015-09-29 | 2017-09-22 | 中国科学院植物研究所 | A kind of intermittent immersed lily bud scale propagation quick-breeding method |
| CN106069342A (en) * | 2016-07-01 | 2016-11-09 | 新疆维吾尔自治区水土保持生态环境监测总站 | A kind of Populus euphratica seedling cultural method and device thereof |
| CN106069342B (en) * | 2016-07-01 | 2022-04-15 | 新疆维吾尔自治区水土保持生态环境监测总站 | Populus diversifolia seedling culture method and device |
| CN106172009B (en) * | 2016-08-15 | 2018-08-21 | 上海离草科技有限公司 | Plant sugar-free culture systems and plant cultivation container |
| CN106172009A (en) * | 2016-08-15 | 2016-12-07 | 上海离草科技有限公司 | Plant sugar-free culture systems and plant cultivation container |
| CN107318646A (en) * | 2017-05-22 | 2017-11-07 | 江苏绿洲园艺绿化有限公司 | One kind comb lip stem of noble dendrobium seedling fast breeding method |
| CN107896964A (en) * | 2017-10-30 | 2018-04-13 | 宁波神乙草生物科技有限公司 | Dendrobium candidum breeding apparatus |
| CN109169242A (en) * | 2018-09-30 | 2019-01-11 | 河北科技师范学院 | A kind of water culture basin alms bowl, column and culture apparatus |
| CN109169242B (en) * | 2018-09-30 | 2021-07-09 | 河北科技师范学院 | Water planting basin alms bowl, stand and cultivation device |
| CN109644617A (en) * | 2019-01-09 | 2019-04-19 | 贵州省旱粮研究所 | A kind of layering culture dish and its transfer tool handgrip |
| CN109644617B (en) * | 2019-01-09 | 2022-02-01 | 贵州省旱粮研究所 | Layered culture dish and transfer tool tongs thereof |
| CN111771708A (en) * | 2020-07-14 | 2020-10-16 | 深圳市航天食品分析测试中心有限公司 | Indoor dendrobium nobile soilless culture device and method thereof |
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| CN104823824B (en) | 2017-07-28 |
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