CN104823824B - The intermittent immersed cultural method and culture apparatus of the stem of noble dendrobium - Google Patents
The intermittent immersed cultural method and culture apparatus of the stem of noble dendrobium Download PDFInfo
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- CN104823824B CN104823824B CN201510225344.7A CN201510225344A CN104823824B CN 104823824 B CN104823824 B CN 104823824B CN 201510225344 A CN201510225344 A CN 201510225344A CN 104823824 B CN104823824 B CN 104823824B
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- Prior art keywords
- stem
- noble dendrobium
- intermittent
- epicoele
- seedling
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G2031/006—Soilless cultivation, e.g. hydroponics with means for recycling the nutritive solution
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The intermittent immersed cultural method and device of a kind of stem of noble dendrobium, by the sterile dendrobe seedling taken root, it is transferred in intermittent immersed culture apparatus, Liquid Culture is carried out under field conditions (factors), not sugaring in nutrient solution, also it is not required to be passed through carbon dioxide, the carbon source needed for stem of noble dendrobium growth comes from the photosynthesis of itself.Using the method for the present invention, stem of noble dendrobium condition of culture is simplified, planting cost is reduced.
Description
Technical field
The present invention relates to biotechnology, the intermittent immersed cultural method of more particularly to a kind of stem of noble dendrobium and culture are filled
Put.
Technical background
The stem of noble dendrobium is the nourishing class Chinese herbal medicine precious simply of China, and with tonifying Qi stomach invigorating, nourishing kidney yin strengthens human immunity
The effect of power,《Sheng Nong's herbal classic》In be classified as top grade.Because consumption is huge, two crises are faced with present:One is wild plant
Resource reserves and yield generally existing downward trend；Two be that stem of noble dendrobium demand is continuously increased, and is caused to the medicinal plant
The excess excavation of resource.Traditional dendrobe cultivation technology mainly includes tree inoculated and cultured living and soilless substyate culture.Tree inoculation living
Cultivate yield too low；Matrix used has not only broken up the tree resources of preciousness based on pine bark during soilless substrate culture, and
Add planting cost.By being found to the retrieval of prior art, Jiang Ping etc. (2013) invented it is a kind of " a kind of dendrobium candidum without
Sugared method for tissue culture ", Xiao Yulan etc. (2012) inventions " a kind of cultural method of saccharide-free box-type plant tissue ", Wang Xilin etc.
(2006) a kind of " Aeroponic type plant sugar-free tissue culturing device " has been invented, these patents of invention can reduce the sterile tissue-cultured seedling of the stem of noble dendrobium
The problem of pollution rate, but during without solving to grow under the outer natural conditions of stem of noble dendrobium seedling room, required matrix.In addition, the stem of noble dendrobium is to root
Ventilation requirement is high, and plant long period of soaking causes rotten in a liquid, easily in above-mentioned patent；In addition carbon dioxide is passed through toward bottom
Planting cost can be increased.
The content of the invention
The purpose of the present invention, exactly in order to overcome the problem of above-mentioned prior art is present, there is provided a kind of leaching of the interval of stem of noble dendrobium
Do not have formula cultural method and culture apparatus.
In order to realize the purpose of the present invention, present invention employs following technical scheme：A kind of intermittent immersed training of the stem of noble dendrobium
The method of supporting, comprises the following steps：
First, stem of noble dendrobium seed is carried out disinfection with mercuric chloride solution, be then seeded into seed germination medium, trained under illumination
Support；
2nd, bottle cap is opened after sterile seedling rooting, practices seedling 2-3 days；
3rd, practice the agar that root residual is cleaned after seedling terminates, be then transferred in intermittent immersed culture apparatus；
4th, nutrient solution is injected in intermittent immersed culture apparatus cavity of resorption, then the sterile cuttage of stem of noble dendrobium seedling to device epicoele
And fixed with stainless steel mesh seedbed；Stem of noble dendrobium seedling is cultivated using intermittent immersed training mode, nutrient solution is allowed in siphon
Intermittent cyclic is shifted between the epicoele and cavity of resorption of device under the collective effect of pipe and liquid pump.
Seed germination medium described in step one, to add the MS solid mediums of 0.2-0.5mg/L gibberellin.
Intermittent immersed training mode is described in step 4, at room temperature, and intermittent immersed culture apparatus is placed in into outdoor
Shady place, it is to avoid direct sunlight, it is not necessary to gnotobasis, the periodic Control of nutrient solution intermittent cyclic transfer was at 30-60 minutes, often
Change a fresh nutrient solution within 15-20 days.
The 1/2MS sugar-free culture-mediums that the formula of the nutrient solution halves for a great number of elements, sugared part comes from needed for stem of noble dendrobium growth
Photosynthesis.
For the culture apparatus of the intermittent immersed cultural method of the above-mentioned stem of noble dendrobium, including incubator, the incubator is by one piece
Dividing plate is separated into epicoele and cavity of resorption, and communicating pipe, siphon pipe and liquid pump are connected between epicoele and cavity of resorption, is also set in epicoele
There is the stainless steel mesh seedbed for anchor stone dry measure used in former times seedling.
The siphon port of the siphon pipe is close to the bottom of chamber of epicoele.
Using apparatus and method of the present invention, by the sterile dendrobe seedling taken root, an intermittent immersed culture is transferred to
In device, not sugaring in Liquid Culture, nutrient solution is carried out under field conditions (factors), is also not required to be passed through carbon dioxide, stem of noble dendrobium growth institute
The carbon source needed comes from the photosynthesis of itself, simplifies condition of culture, reduces planting cost.
Brief description of the drawings
Fig. 1 is the structural representation of the intermittent immersed culture apparatus of the stem of noble dendrobium.
Specific implementation method
The intermittent immersed cultural method of the stem of noble dendrobium of the present invention, comprises the following steps：
First, stem of noble dendrobium seed is carried out disinfection with mercuric chloride solution, be then seeded into seed germination medium, indoor illumination
Lower culture；Seed germination medium is the MS solid mediums of addition 0.5mg/L gibberellin.
2nd, bottle cap is opened after sterile seedling rooting, practices seedling 2 days；
3rd, practice the agar that root residual is cleaned after seedling terminates, be then transferred in intermittent immersed culture apparatus and cultivated.
4th, at room temperature, intermittent immersed culture apparatus is placed in outdoor shady place, it is to avoid direct sunlight.Soaked in interval
Do not have formula culture apparatus cavity of resorption to inject nutrient solution, the 1/2MS sugar-free culture-mediums that the formula of nutrient solution halves for a great number of elements, then
The sterile cuttage of stem of noble dendrobium seedling is fixed to device epicoele and with stainless steel mesh seedbed 5；Open liquid pump 3, effect of the nutrient solution in liquid pump
Under be transferred to epicoele from the cavity of resorption of container, when epicoele liquid liquid level exceed siphon pipe 4 peak when (siphon pipe 4 is most
High point is higher than the peak of stem of noble dendrobium seedling, it is ensured that all stem of noble dendrobium seedlings of nutrition liquid energy submergence), the nutrient solution of container epicoele is in siphonage
Under be quickly transferred to below container.In this way, nutrient solution reciprocation cycle between the upper and lower chamber of container is shifted, and stem of noble dendrobium seedling will
Soaked in liquid and realize intermittent immersed culture with conditions of exposed be alternately present.The cycle of nutrient solution intermittent cyclic transfer
Control changed a fresh nutrient solution per 15-20 days at 30-60 minutes.
Culture apparatus used in the present embodiment is as shown in figure 1, including incubator 1, the incubator is separated into by one piece of dividing plate
Epicoele 11 and cavity of resorption 12, communicating pipe 2, siphon pipe 4 and liquid pump 3 are connected between epicoele and cavity of resorption, is additionally provided with epicoele
Stainless (steel) wire 5 for cuttage stem of noble dendrobium seedling.The siphon port of siphon pipe is close to the bottom of chamber of epicoele.
Incubator is placed in the greenhouse provided with shading screen, and temperature is 20 degree or so, and illumination is natural light.Nutrient solution is a large amount of
The 1/2MS sugar-free culture-mediums that element halves, the resting period is 30 minutes, and nutrient solution was replaced once per 15-20 days.After 30 days, system
Stone solution biomass is counted, as a result growth is slightly slower than traditional solid matrix training mode, but cost is substantially reduced.
Stem of noble dendrobium seedling used comes from this laboratory in the present embodiment, and culture apparatus used is determined with the calm and peaceful glass apparatus factory in Shanghai
Do, liquid pump is provided by Baoding Beng Ye Co., Ltds.
Claims (5)
1. a kind of intermittent immersed cultural method of stem of noble dendrobium, it is characterised in that：Comprise the following steps：
First, stem of noble dendrobium seed is carried out disinfection with mercuric chloride solution, be then seeded into seed germination medium, cultivated under illumination；
2nd, bottle cap is opened after sterile seedling rooting, practices seedling 2-3 days；
3rd, practice the agar that root residual is cleaned after seedling terminates, be then transferred in intermittent immersed culture apparatus；
4th, nutrient solution is injected in intermittent immersed culture apparatus cavity of resorption, then the sterile cuttage of stem of noble dendrobium seedling is used in combination to device epicoele
Stainless steel mesh seedbed is fixed；Stem of noble dendrobium seedling is cultivated using intermittent immersed training mode, allow nutrient solution in siphon pipe and
Intermittent cyclic is shifted between the epicoele and cavity of resorption of device under the collective effect of liquid pump；
Intermittent immersed training mode is described in step 4, at room temperature, and intermittent immersed culture apparatus is placed in into outdoor cool place
Place, it is to avoid direct sunlight, it is not necessary to gnotobasis, the periodic Control of nutrient solution intermittent cyclic transfer was at 30-60 minutes, per 15-
Change a fresh nutrient solution within 20 days.
2. cultural method according to claim 1, it is characterised in that seed germination medium described in step one, for addition
The MS solid mediums of 0.2-0.5mg/L gibberellin.
3. cultural method according to claim 1, it is characterised in that what the formula of the nutrient solution halved for a great number of elements
1/2MS sugar-free culture-mediums, sugared part comes from photosynthesis needed for stem of noble dendrobium growth.
4. the culture apparatus of the intermittent immersed cultural method for the stem of noble dendrobium described in claim 1, it is characterised in that：Including culture
Case, the incubator is separated into epicoele and cavity of resorption by one piece of dividing plate, and communicating pipe, siphon pipe are connected between epicoele and cavity of resorption
And liquid pump, it is additionally provided with the stainless steel mesh seedbed for fixing stem of noble dendrobium seedling in epicoele.
5. culture apparatus according to claim 4, it is characterised in that chamber of the siphon port of the siphon pipe close to epicoele
Bottom.
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| CN201510225344.7A CN104823824B (en) | 2015-05-04 | 2015-05-04 | The intermittent immersed cultural method and culture apparatus of the stem of noble dendrobium |
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| CN104823824B true CN104823824B (en) | 2017-07-28 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105191798B (en) * | 2015-09-29 | 2017-09-22 | 中国科学院植物研究所 | A kind of intermittent immersed lily bud scale propagation quick-breeding method |
| CN106069342B (en) * | 2016-07-01 | 2022-04-15 | 新疆维吾尔自治区水土保持生态环境监测总站 | Populus diversifolia seedling culture method and device |
| CN106172009B (en) * | 2016-08-15 | 2018-08-21 | 上海离草科技有限公司 | Plant sugar-free culture systems and plant cultivation container |
| CN107318646A (en) * | 2017-05-22 | 2017-11-07 | 江苏绿洲园艺绿化有限公司 | One kind comb lip stem of noble dendrobium seedling fast breeding method |
| CN107896964A (en) * | 2017-10-30 | 2018-04-13 | 宁波神乙草生物科技有限公司 | Dendrobium candidum breeding apparatus |
| CN109169242B (en) * | 2018-09-30 | 2021-07-09 | 河北科技师范学院 | Water planting basin alms bowl, stand and cultivation device |
| CN109644617B (en) * | 2019-01-09 | 2022-02-01 | 贵州省旱粮研究所 | Layered culture dish and transfer tool tongs thereof |
| CN111771708A (en) * | 2020-07-14 | 2020-10-16 | 深圳市航天食品分析测试中心有限公司 | Indoor dendrobium nobile soilless culture device and method thereof |
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| CN1188593A (en) * | 1997-01-22 | 1998-07-29 | 中国科学院遗传研究所 | Culture container and method for producing potato miniature potato etc. plant material using container thereof |
| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
| CN103039368A (en) * | 2013-01-27 | 2013-04-17 | 云南天泉生物科技股份有限公司 | Sugar-free tissue culture method of dendrobium officinale |
| CN103270946A (en) * | 2013-02-04 | 2013-09-04 | 南京博方生物科技有限公司 | Dendrobium officinale seedling production and hardening synchronization method |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188593A (en) * | 1997-01-22 | 1998-07-29 | 中国科学院遗传研究所 | Culture container and method for producing potato miniature potato etc. plant material using container thereof |
| CN102835318A (en) * | 2012-10-08 | 2012-12-26 | 荆树汉 | Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition |
| CN103039368A (en) * | 2013-01-27 | 2013-04-17 | 云南天泉生物科技股份有限公司 | Sugar-free tissue culture method of dendrobium officinale |
| CN103270946A (en) * | 2013-02-04 | 2013-09-04 | 南京博方生物科技有限公司 | Dendrobium officinale seedling production and hardening synchronization method |
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