CN1961655A - Air culture type plant sugar-free tissue culture device - Google Patents
Air culture type plant sugar-free tissue culture device Download PDFInfo
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- CN1961655A CN1961655A CNA2006101192409A CN200610119240A CN1961655A CN 1961655 A CN1961655 A CN 1961655A CN A2006101192409 A CNA2006101192409 A CN A2006101192409A CN 200610119240 A CN200610119240 A CN 200610119240A CN 1961655 A CN1961655 A CN 1961655A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention relates to a plant culture device in the technical field of agricultural machinery, in particular to an aeroponic plant sugar-free tissue culture device. The method comprises the following steps: cultivate container, cultivation frame, illumination system, environment detection device, vegetation environment and maintain the system, cultivate the inside from last to setting up a plurality of cultivation framves down of container, the plant body settle with cultivate on the frame, set up illumination system, environment detection device in the cultivation container, vegetation environment maintains the system and arranges the cultivation container outside in, meets with cultivation container through spray line. The invention comprehensively solves the problem of controlling the temperature and the humidity of the tissue culture container in the sugar-free tissue culture process on the premise of ensuring that the illumination and the carbon source of the sugar-free culture micropropagation are fully satisfied, effectively utilizes the space of the tissue culture device and saves energy through improving the structure, overcomes the defects of weak growth and development of plant leaf surfaces and improves the survival rate of plant transplantation.
Description
Technical field
What the present invention relates to is a kind of plant cultivation device of agricultural machinery technological field, particularly a kind of Aeroponic type plant sugar-free tissue culturing device.
Background technology
The modern plants culture technique has had very big breakthrough to traditional plant plantation process aspect, part solves and is solving more all sidedly the pollution rate height that exists in traditional tissue culture, growth and development of plants is bad, the physiology and appearance disorder, deformity, growing delays or problem such as death, regulates the environmental condition of improving plant growing by artificial environment in the prior art, by replenishing of good illumination and carbon source and other nutrition, make plant have the survival rate of growth rate and Geng Gao faster.Scientific and technical personnel further improve survival rate of plant by continuous adjustment technology condition and improvement art designs at present, effectively improve plant variety, effectively energy savings and institute take up space, this is to improving the agriculture production technology level, ensure the fine quality of species, the diversity of species is improved the ecological environment, increase farmers' income, be significant.
In the prior art document, International PCT patent publication No. is: WO 2005/058022 A1, patent application is artificial: Zhang Yongtai, name is called: combined type plant sugar-free tissue-culturing quick-propagation devices and methods therefor, and this patented technology summary is: culture vessel is made with transparent material, and its peripheral cross section is an equilateral hexagon, be the sealing shape, several combinations of the culture vessel of equilateral hexagon are honeycomb arrangement, nutrient solution cross flow device, nanometer light are set, humidity conditioner, CO in the culture vessel
2Device is arranged on outside the culture vessel, is connected in the culture vessel by pipeline respectively, and blower fan gas outlet and return join, and is connected with temperature control equipment again.
Name is called: the prior art of combined type plant sugar-free tissue-culturing quick-propagation devices and methods therefor belongs to the hydroponic plant category, the hydroponic plant root system is subject to dissolved oxygen levels in the nutrient solution for the absorption of moisture content and mineral nutrition, when the dissolved oxygen content in the cultivating nutrient solution is low, although root system is that direct impregnation is in supporting liquid, but can influence normally carrying out of aerobic respiration because of oxygen is not enough, thereby make root system can not obtain more respiratory metabolism transformation energy, can not bring into play best root system absorption function, have only the dissolved oxygen in the nutrient solution to reach suitable or super oxygen enrichment state, the advantage that just can make root system absorb nutrition and moisture content is able to maximum performance, that is to say that the dissolved oxygen in the nutrient solution is the limiting factor of most critical in the water culture technology.Simultaneously, the prior art directly places culture vessel inside with the plant nanometer light, and the heat energy that is produced in its illumination process has directly influenced the variations in temperature of culture vessel, makes plant corpus growing environment day and night temperature big.The prior art is because with CO
2Gas is directly by in the air inlet pipe input culture vessel, at CO
2Under the specific gravity of gas influence, CO in the culture vessel
2Gas presents non-uniform phenomenon (CO up and down
2Gas is at culture vessel top concentration height, and bottom concentration is low), big to the plant strain growth influence factor, easily form the otherness of top and the bottom plant.In addition, the prior art is regulated the temperature in the culture vessel, humidity, only passes through CO
2The gas humidification of lowering the temperature, because the restriction of the gas concentration standard in the culture vessel, the prior art can not continue to carry out the cool-down dehumidification operation, has great limitation aspect the environment control.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, a kind of Aeroponic type plant sugar-free tissue culturing device is provided.The present invention is guaranteeing that sugar-free cultivates under the illumination of micropropagation and the prerequisite that carbon source attains full and complete satisfaction, comprehensively solved in the sugar-free group training process the temperature of group culture container, the control problem of humidity, by improvement to structure, the space and the energy savings of group training device have been effectively utilized, overcome the plant leaf surface fragility of growing, improved the survival rate of plantlet of transplant.The present invention adopts aerosol to cultivate to plant, can make plant corpus under the sugar-free culture environment, and it is best that plant growth state reaches.Effectively improve the space availability ratio and unit interval plant cultivation quantity of culture vessel, be on a large scale, resources conservation optimisation technique cheaply.
The present invention is achieved by the following technical solutions, the present invention includes: culture vessel, culturing rack, illumination system, plant growth environment maintenance system, environmental detection set, culture vessel inside is provided with some culturing racks from top to bottom, plant corpus can settle or be attached to culturing rack on, illumination system, environmental detection set are set in the culture vessel, the plant growth environment maintenance system places the culture vessel outside, joins by spray pipe and culture vessel.
Illumination system of the present invention comprises light emitting diode, lamp box, blower fan, and light emitting diode places lamp box interior lamp box and blower fan to join, and blower fan and culture vessel join, and places the culturing rack top.The plant growth environment maintenance system is put and is comprised CO
2Source of the gas, magnetic valve, nutrient solution atomizing device, cooling device, air pump, spray pipe.CO
2Source of the gas joins by pipeline and magnetic valve, and magnetic valve joins by pipeline and nutrient solution atomizing device, and nutrient solution atomizing device joins by pipeline and air pump, and air pump joins by pipeline and culture vessel and extends to culture vessel inside.Environmental detection set comprises temperature, humidity, illuminance detection probe, and environmental detection set and magnetic valve and air pump join, and are installed in the culture vessel.
Can plant normally or fast grow, and mainly determined that by two big metabolism one is to be the photosynthetic metabolite at center with branches and leaves, and another is to be the absorption metabolism at center with the root system.The present invention is with CO
2The gas circulation pipeline and the nutrient solution circulatory system are carried out function and are merged, and handle that by the plant growth environment maintenance system of culture vessel outside nutrient solution is atomized, thereby the liquid state that nutrient solution is come become the mist attitude, the nutrient solution of mist attitude can with CO
2Gas enters culture vessel with spray pipe, the CO of prior art
2Gas is after entering culture vessel, because CO
2Gas is big than other specific gravity of gas, the CO in the culture vessel
2Gas mainly falls to bottom of culture vessel, makes the CO in the culture vessel
2The gas concentration skewness causes the CO of culture vessel top plant environment of living in
2Gas concentration is low, and bottom CO
2The energy imbalance that gas concentration is high.Prior art with blower fan by the tracheae CO that temperature in the culture vessel is too high
2Gas is extracted out, thereby reaches cool effect.Because lack flowability, its cooling-down effect is unsatisfactory, when the temperature in the culture vessel was higher than ambient temperature, the easy condensing water droplet of culture vessel inwall increased air humidity simultaneously, and gas humidity is wayward.On the other hand, the nutrient solution cross flow mode that prior art adopts, because plant root growth is irregular, easily cause the skewness of nutrient solution on nutrient solution cross flow winding stair, cause the plant corpus growth of culture vessel top and bottom inhomogeneous, plant corpus produces difference, and nutrient solution concentration, acid-base value, temperature and moisture storage can changes in flow process, and the growth of plant is had a negative impact.
The present invention has obviously simplified prior art CO
2The gas circulation pipeline, increased the culture vessel volume inside, the complicated pipe-line system with nutrient solution circulatory system has been arranged again.The present invention is suspended from the plant corpus root system in the atomization space of a high humility, nutrient solution adopts the atomizing feed liquid way, suppressing under the environment of spraying, can allow nutrient solution evenly be distributed in plant corpus root surface in time on the one hand, simultaneously, water is in atomization process, can allow water clusters diminish, be split into simple coupling or unimolecule water, root system of plant is had a significant effect for the promotion that moisture content absorbs, the absorption efficiency of nutrition and moisture content is greatly improved.
Environmental detection set of the present invention is according to temperature in the culture vessel and CO
2Gas concentration starts magnetic valve according to setting value, and magnetic valve can be with CO after starting
2CO in the source of the gas
2Gas timing, quantitative suction nutrient solution atomizing device, CO
2Gas is extracted out through spray pipe to culture vessel by air pump, and under the swabbing pressure of air pump, is injected into the plant corpus root that is positioned on the culturing rack with after atomizing shape nutrient solution gas in the nutrient solution atomizing device mixes.When temperature in the culture vessel was higher than setting value, environmental detection set promptly started cooling device, and being wrapped in the outer field cooling device of spray pipe can be with mist (atomizing shape nutrient solution gas and the CO through spray pipe
2Gas) remove the temperature cooling, when entering culture vessel the plant corpus root is sprayed, can handle, also have the humidification effect to culture vessel inside simultaneously implementing cooling in the culture vessel.Illumination system provides photosynthesis required environment for plant corpus.
The heat energy that the present invention is produced because of the prior art nanometer light makes temperature rising in the culture vessel, and the more high reason of cooling cost has been improved employing plant nanometer light.The present invention since cooling gas with of the injection of atomizing shape nutrient solution gas at culture vessel, its spray regime can fill the air to whole culture vessel, does not have cooling " dead angle " in the culture vessel, the efficient of lowering the temperature height.The present invention adopts intermittently atomizing type can scientifically supply with required moisture content of plant growing and nutrition, and is directly to be sprayed onto the root system surface, can allow root system absorb with the most direct the selection the most apace, has guaranteed humidity and temperature in the culture vessel.
The aeroponic type sugar-free tissue culture technology that the present invention adopts can allow the root configuration of plant corpus and growth pattern be able to fullest to embody the most freely, usually the root configuration of plant can show different root configurations because of the difference of rhizosphere environment, different root configurations absorbs nutrition for root system and moisture content has different efficient and adaptability, under the aerosol environment, the root configuration is the dichotomy configuration of adventive root mostly, it is the most direct with moisture content that this configuration absorbs nutrition, and the path of transportation is the shortest, progression is minimum is more conducive to nutrition fast and turns round.And no matter be woody or herbaceous plant, in case place the aerosol space, its mode that goes out root and classification will change immediately, form a large amount of adventive root, this adventive root has the double grading of water root and respiratory root, has extremely strong respiration function, and some plant also can form a large amount of root hairs, this hair educated all more flourishing than any planting type, have the widest surface area of the nutrition of absorption, the formation of these root morphologies and configuration only just can be grown in unrestricted unresisted space environment.
The root system of most of plants can keep comparatively flourishing parenchyma in the aerosol environment, have stronger regeneration and repair ability, if it is carried out root pruning, can be recovered with the fastest speed, this has also created convenience for physiological regulating control and Growth Control, can regulate and control physiology and growth by root system pruning.The adventive root root system of this prosperity, under the aerosol environment, the elongation zone speciality of root, this also is growth fast and the physiological foundation of repairing.
Aeroponic type sugar-free tissue culture technology of the present invention is that best environment has been created in the expression of the growing of root system of plant, physiological metabolism, functional gene, use the present invention and carry out the plant aerial fog cultivation, plant growth rate is than the fast 3-5 of substrate culture times, than the fast 1.5-3 of water planting doubly.Rooting rate of the present invention reaches 100%, it is fast to take root, the root system of plant prosperity is white in color, fast 1.5 times of average leaf area growth rate than prior art, the plant leaf area on average improves 1.5 times, the number of sheets many 37% than prior art, robust plant, fresh weight average out to 575mg/ strain, plant does not have yellow and glass seedling phenomenon produces.Simultaneously, because at the outside plant growth environment maintenance system that adopts of culture vessel, and culture vessel inside only comprises illumination system and detection system, make the space availability ratio of culture vessel that significantly lifting be arranged, single cover plant growth environment maintenance system can be used in combination with a plurality of culture vessels, the utilization of plant growth environment maintenance system is significantly improved, and cultivate with low cost.
Description of drawings
Fig. 1 Facad structure schematic diagram of the present invention
Fig. 2 side structure schematic diagram of the present invention
Embodiment
Below in conjunction with accompanying drawing embodiments of the invention are elaborated: present embodiment has provided detailed embodiment and process being to implement under the prerequisite with the technical solution of the present invention, but protection scope of the present invention is not limited to following embodiment.
As shown in Figure 1 and Figure 2, present embodiment comprises: culture vessel 1, culturing rack 2, illumination system 3, plant growth environment maintenance system 4, environmental detection set 5, its connected mode is: culture vessel 1 inside is provided with some culturing racks 2 from top to bottom, culturing rack 2 can be irregular alignment, plant corpus can settle or be attached to culturing rack 2 on, illumination system 3, environmental detection set 5 are set in the culture vessel 1, plant growth environment maintenance system 4 places culture vessel 1 outside, joins by spray pipe and culture vessel 1.Culture vessel 1 is determined its space layout area according to the expulsion pressure of spray pipe, and culture vessel 1 is made with sun sheet material matter, can be the operation of electric energy supply light lighting system with conversion of solar energy.
Plant growth environment maintenance system 4 comprises CO
2Source of the gas 9, magnetic valve 10, nutrient solution atomizing device 11, cooling device 12, air pump 13, spray pipe 14, CO
2Source of the gas 9 joins by spray pipe 14 and magnetic valve 10, magnetic valve 10 joins by spray pipe 14 and nutrient solution atomizing device 11, nutrient solution atomizing device 11 joins by spray pipe 14 and air pump, and air pump 13 joins by spray pipe 14 and culture vessel 1 and extends to culture vessel 1 inside.Cooling device 12 is the heat radiation sheet, and cooling device 12 is wrapped in spray pipe 14 outsides that nutrient solution atomizing device 11 and air pump 13 join.Magnetic valve 10 joins with environmental detection set 5, and air pump 13 joins with environmental detection set 5.
Adopt same nutrient solution prescription, identical seedling and field planting time, or even nutrient solution have identical dissolved oxygen EC temperature, present embodiment adopts air culture environment controlling technique planting type, quality has clear superiority between the plant of cultivating than water planting (nutrient solution cross flow) mode of prior art, show as growth rate, the high temperature resistance high light, evident difference has all appearred in the non-oxidizability of root system etc.
Present embodiment has guaranteed that humidity in the culture vessel 1 and temperature nutrient solution fill the air the whole space to rhizosphere environment and culture vessel, allows root system that sufficient moisture guarantee is arranged, and can not occur excess moisture or the physiological absorption obstacle when very few as soil cultivation.And adopt this moisture content supply mode (humidification), can improve the availability of water substantially.Simultaneously, environmental detection set 5 starts 10 couples of CO of magnetic valve according to setting value
2Gas carries out regularly, quantitatively control, realizes carbon dioxide content being controlled accurately its CO
2Gas concentration distributing precision scope can reach 0.3-19.9%, CO
2The gas input pressure is 0.3kg/cm
2
The present embodiment rooting rate reaches 100%, and it is fast to take root, and the root system of plant prosperity is white in color, fast 1.5 times than prior art of average leaf area growth rate., present embodiment is with in 20 days the culture period of flameray gerbera cultivation, and the plant leaf area on average can reach 1.5cm
2, improve 1.5 times than prior art.The number of sheets is many 37% than original device, robust plant, fresh weight average out to 575mg/ strain (prior art: the 412mg/ strain), be transited into motility rate and reach that (prior art: 83%), plant does not have yellow, glass seedling phenomenon produces more than 98%.
Claims (10)
1, a kind of Aeroponic type plant sugar-free tissue culturing device, comprise: culture vessel (1), culturing rack (2), illumination system (3), environmental detection set (5), it is characterized in that, also comprise: plant growth environment maintenance system (4), culture vessel (1) inside is provided with some culturing racks (2) from top to bottom, plant corpus be placed in culturing rack (2) on, illumination system (3), environmental detection set (5) are set in the culture vessel (1), plant growth environment maintenance system (4) places culture vessel (1) outside, joins by spray pipe and culture vessel (1).
2, Aeroponic type plant sugar-free tissue culturing device according to claim 1 is characterized in that, described plant growth environment maintenance system (4) comprises CO
2Source of the gas (9), magnetic valve (10), nutrient solution atomizing device (11), cooling device (12), air pump (13), spray pipe (14), CO
2Source of the gas (9) joins by spray pipe (14) and magnetic valve (10), magnetic valve (10) joins by spray pipe (14) and nutrient solution atomizing device (11), nutrient solution atomizing device (11) joins by spray pipe (14) and air pump, air pump (13) joins by spray pipe (14) and culture vessel (1) and extends to culture vessel (1) inside, and cooling device (12) is wrapped in spray pipe (14) outside that nutrient solution atomizing device (11) and air pump (13) join.
According to claim 1 or 2 described Aeroponic type plant sugar-free tissue culturing devices, it is characterized in that 3, magnetic valve (10) and air pump (13) in the described plant growth environment maintenance system (4) join with environmental detection set (5) respectively.
4, Aeroponic type plant sugar-free tissue culturing device according to claim 1, it is characterized in that, described illumination system (3) comprises light emitting diode (6), lamp box (7), blower fan (8), light emitting diode (6) places lamp box (7) inside, lamp box (7) joins with blower fan (8), blower fan (8) joins with culture vessel (1), and places culturing rack (2) top.
5, Aeroponic type plant sugar-free tissue culturing device according to claim 4 is characterized in that, described blower fan (8) forms closed structure with lamp box (7),
6, Aeroponic type plant sugar-free tissue culturing device according to claim 4 is characterized in that, described light emitting diode (6) red light emitting diodes: the blue LED proportion of installation is 2: 1.
7, Aeroponic type plant sugar-free tissue culturing device according to claim 6 is characterized in that, the red light emitting diodes frequency of light wave is 660nm.
8, Aeroponic type plant sugar-free tissue culturing device according to claim 6 is characterized in that, the blue LED frequency of light wave is 450nm,
9, Aeroponic type plant sugar-free tissue culturing device according to claim 1 is characterized in that, environmental detection set (5) is a detection probe, and detection probe is installed in the culture vessel (1).
According to claim 1 or 9 described Aeroponic type plant sugar-free tissue culturing devices, it is characterized in that 10, environmental detection set (5) joins with magnetic valve (10) and air pump (13).
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| CNB2006101192409A CN100464632C (en) | 2006-12-07 | 2006-12-07 | Air culture type plant sugar-free tissue culture device |
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|---|---|---|---|
| CNB2006101192409A CN100464632C (en) | 2006-12-07 | 2006-12-07 | Air culture type plant sugar-free tissue culture device |
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Cited By (14)
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| CN102084813A (en) * | 2010-12-23 | 2011-06-08 | 福建永安天奇健金线莲生态实业有限公司 | Method for culturing sugar-free tissue of jewel orchid |
| CN102342237A (en) * | 2010-07-29 | 2012-02-08 | 任路 | Aeroponics organic vegetable production technique |
| CN102349446A (en) * | 2011-08-29 | 2012-02-15 | 镇江盛弘景观植物有限公司 | Sugar-free micropropagation tissue culture method of plant |
| CN102415326A (en) * | 2011-09-15 | 2012-04-18 | 袁强 | Seedling raising pool and seedling raising method |
| CN103650994A (en) * | 2012-09-04 | 2014-03-26 | 大汉光电股份有限公司 | Air purification and oxygenation type plant cultivation system |
| CN104186299A (en) * | 2014-08-29 | 2014-12-10 | 中山市黄加蓝农业科技有限公司 | Plant growth machine |
| CN105052462A (en) * | 2015-07-30 | 2015-11-18 | 黄旭胜 | Air culture and rapid propagation method of tomatoes |
| CN105123488A (en) * | 2015-08-28 | 2015-12-09 | 肖玉兰 | Plant tissue culture rooting and seedling exercising integrated cultivating device and cultivating method thereof |
| CN105210839A (en) * | 2015-11-03 | 2016-01-06 | 北京农业生物技术研究中心 | A kind of atomizing Vitro Plant culture systems and cultural method |
| CN105532314A (en) * | 2016-01-11 | 2016-05-04 | 中国农业科学院作物科学研究所 | Low-concentration carbon dioxide incubator for basic scientific research and control method |
| CN106417028A (en) * | 2016-10-14 | 2017-02-22 | 屏南县惠荣农业科技有限公司 | Succulent tissue culture container |
| CN107926682A (en) * | 2017-11-27 | 2018-04-20 | 杭州蓝匠光环境科技有限公司 | Vertical planting system |
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| CN113875593A (en) * | 2021-10-22 | 2022-01-04 | 杭州木木生物科技有限公司 | Sugar-free tissue culture method and device for improving rooting rate of nandina domestica |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1051575C (en) * | 1996-05-15 | 2000-04-19 | 中国科学院化工冶金研究所 | Reactor for nutrient liquid atomizing culture of plant |
| WO2005058022A1 (en) * | 2003-12-19 | 2005-06-30 | Yongtai Zhang | Combination-type plant sugarless tissue culture propagation device and method thereof |
| CN2666144Y (en) * | 2003-12-19 | 2004-12-29 | 章永泰 | Plant group culturing and fast propagating combine system |
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2006
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