CN100493336C - Cultivation process of Mnium cuspidaum Hedw - Google Patents
Cultivation process of Mnium cuspidaum Hedw Download PDFInfo
- Publication number
- CN100493336C CN100493336C CNB2006100129427A CN200610012942A CN100493336C CN 100493336 C CN100493336 C CN 100493336C CN B2006100129427 A CNB2006100129427 A CN B2006100129427A CN 200610012942 A CN200610012942 A CN 200610012942A CN 100493336 C CN100493336 C CN 100493336C
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- hedw
- cuspidaum
- mnium
- nutrient solution
- solution
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- Y02P60/216—
Abstract
The cultivation process of Mnium cuspidaum Hedw includes the following steps: collecting wild Mnium cuspidaum Hedw, drying in the air, grinding and adding distilled water to compound water solution of Mnium cuspidaum Hedw in 10-30 % concentration; loading moist culture medium into container, spreading the Mnium cuspidaum Hedw solution to the surface and covering the container mouth with holed plastic film; setting the container under indoor natural lighting, controlling the temperature to 16-22 deg.c and pouring nutritious solution every other day; and pouring nutritious solution and water alternately for the plant to grow for 10-30 days. The cultivation process is simple, practical, low in cost, high in plant survival rate and suitable for large area cultivation in greenhouse to provide great amount of Mnium cuspidaum Hedw.
Description
Technical field
The present invention relates to the artificial cultivation method of bryophyte, specifically is a kind of cultivation method of Mnium cuspidaum Hedw.
Background technology
Bryophyte is that vegetative kingdom is by aquatic transitional type to Lu Sheng, because most of kind bodies are small, simple in structure, their effect and easily out in the cold with people's the relations of production, in fact, bryophyte not only has certain effect at occurring in nature, and also very close with relationship.
It is one of botanic pioneer at occurring in nature, on preventing erosion, play an important role, to the landization in lake, marsh and the bogginess on land, play important succession effect, can also be as comprehensive indicator plant under some living conditions.
Aspect bryophytes such as the monitoring of industry, agricultural, medicine, gardening, atmospheric pollution, biological studies very high economic use value is arranged.
Mnium cuspidaum Hedw (M.cuspitatum Hedw.) is a kind of of bryophyte.Its all herbal medicine can stop blooding, and cures mainly nosebleed epistaxis, woman uterine bleeding, burn; Lymphocytic leukemia and neurogliocytoma there is inhibitory action.
The cultivation of Mnium cuspidaum Hedw successfully can be making full use of of its above-mentioned economic worth and gives security.
The laboratory cultures bryophyte is adopted strict method for plant tissue culture (Wu Pengcheng chief editor " bryophyte biology ") more at present, method is as follows: selected plant gametophyte or sporophyte must be earlier through specific thimerosal sterilizations, clean with aseptic water washing then, make sterile suspension again, in the clean platform of behaviour, be inoculated into defined medium, put illumination box or climatic cabinate, set strict temperature, humidity, numerical value such as light application time and intensity.This method condition is tight, and the cost height is unfavorable for that the general population operates, and makes to promote to be restricted.
Summary of the invention
The object of the present invention is to provide a kind of simple to operate, convenient and easy, survival rate of plant height, the simple and easy cultivation method of the Mnium cuspidaum Hedw of being convenient to promote.
The cultivation method of a kind of Mnium cuspidaum Hedw provided by the invention comprises the steps: field acquisition, the Mnium cuspidaum Hedw that dries are naturally ground in mortar, and adding distil water is made into the Mnium cuspidaum Hedw aqueous solution of 10%-30%; Moistening cultivation matrix is packed in the container, then the Mnium cuspidaum Hedw aqueous solution is sprinkled upon the cultivation matrix surface equably, vessel port covers transparent plastic film with holes; Cultivating container is placed the local natural lighting of indoor light abundance, 16-22 ℃ of control temperature watered nutrient solution every other day again; After plant grows, intersect to water every other day and pour water and nutrient solution, keep matrix moistening, cultivated 10-30 days.It is the peat of 1:1-2 and the mixed thing of vermiculite that described cultivation matrix is selected from pine needle, vermiculite or weight ratio; Described nutrient solution is Knop nutrient solution or the former growth matrix of Mnium cuspidaum Hedw leaching liquor; Described cultivating container is a culture dish, triangular flask, and transparent vessel such as Cans are convenient to daylighting and observation.
A kind of simple and easy cultivation method of Mnium cuspidaum Hedw provided by the invention, compare with the Plant Tissue Breeding method of the commonly used strictness in laboratory, overcome the harsh conditions of its sterile working, simple to operate, convenient and easy, with low cost, method is grasped easily, and the survival rate of plant height is convenient to commerial growing popularizations such as booth, thereby for making full use of of Mnium cuspidaum Hedw economic worth provides technical support and assurance, market prospects are wide.
Embodiment
Embodiment 1
Field acquisition, the Mnium cuspidaum Hedw that dries are naturally ground in mortar, adding distil water is made into 10% solution, moistening cultivation matrix is packed in the culture dish, be sprinkled upon moistening pine needle cultivation matrix surface then equably, the culture dish mouth covers transparent plastic film with holes, culture dish is placed the local natural lighting of indoor light abundance, 16-22 ℃ of control temperature watered nutrient solution every other day again; After plant grows, intersect to water every other day and pour water and nutrient solution, keep matrix moistening, cultivated 10 days.
Embodiment 2
Field acquisition, the Mnium cuspidaum Hedw that dries are naturally ground in mortar, adding distil water is made into 30% solution, moistening cultivation matrix is packed in the triangular flask, be sprinkled upon moistening vermiculite cultivation matrix surface then equably, the plastic film in triangle bottleneck cover tape hole, triangular flask is placed the local natural lighting of indoor light abundance, 16-22 ℃ of control temperature watered nutrient solution every other day again; After plant grows, intersect to water every other day and pour water and nutrient solution, keep matrix moistening, cultivated 15 days.
Embodiment 3
Field acquisition, the Mnium cuspidaum Hedw that dries are naturally ground in mortar, adding distil water is made into 20% solution, moistening cultivation matrix is packed in the Cans, be sprinkled upon the mixture cultivation matrix surface of moistening peat and vermiculite 1:1 then equably, the plastic film in can bottleneck cover tape hole, Cans are placed the local natural lighting of indoor light abundance, 16-22 ℃ of control temperature watered nutrient solution every other day again; After plant grows, intersect to water every other day and pour water and nutrient solution, keep matrix moistening, cultivated 25 days.
Embodiment 4
Field acquisition, the Mnium cuspidaum Hedw that dries are naturally ground in mortar, adding distil water is made into 15% solution, moistening cultivation matrix is packed in the Cans, be sprinkled upon the mixture cultivation matrix surface of moistening peat and vermiculite 1:2 then equably, the plastic film in can bottleneck cover tape hole, Cans are placed the local natural lighting of indoor light abundance, 16-22 ℃ of control temperature watered nutrient solution every other day again; After plant grows, intersect to water every other day and pour water and nutrient solution, keep matrix moistening, cultivated 20 days.
Claims (1)
1. the cultivation method of a Mnium cuspidaum Hedw comprises the steps: field acquisition, the Mnium cuspidaum Hedw that dries are naturally ground in mortar, and adding distil water is made into the Mnium cuspidaum Hedw aqueous solution of 10%-30%; Moistening cultivation matrix is packed in the container, then the Mnium cuspidaum Hedw aqueous solution is sprinkled upon the cultivation matrix surface equably, vessel port covers transparent plastic film with holes; Cultivating container is placed the local natural lighting of indoor light abundance, 16-22 ℃ of control temperature watered nutrient solution every other day again; After plant grows, intersect to water every other day and pour water and nutrient solution, keep matrix moistening, cultivated 10-30 days; It is the peat of 1:1-2 and the mixed thing of vermiculite that described cultivation matrix is selected from pine needle, vermiculite or weight ratio; Described nutrient solution is the Knop nutrient solution.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CNB2006100129427A CN100493336C (en) | 2006-07-12 | 2006-07-12 | Cultivation process of Mnium cuspidaum Hedw |
Applications Claiming Priority (1)
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CNB2006100129427A CN100493336C (en) | 2006-07-12 | 2006-07-12 | Cultivation process of Mnium cuspidaum Hedw |
Publications (2)
Publication Number | Publication Date |
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CN1887066A CN1887066A (en) | 2007-01-03 |
CN100493336C true CN100493336C (en) | 2009-06-03 |
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CNB2006100129427A Expired - Fee Related CN100493336C (en) | 2006-07-12 | 2006-07-12 | Cultivation process of Mnium cuspidaum Hedw |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103069989A (en) * | 2013-01-22 | 2013-05-01 | 山西大学 | Method for producing esthetic bryophyte bottle |
CN111053025A (en) * | 2019-12-30 | 2020-04-24 | 江西师范大学 | Large-scale artificial propagation method of physcomitrella acuminate |
CN115299329A (en) * | 2022-08-04 | 2022-11-08 | 内蒙古农业大学 | Method for culturing odontopathy and application thereof |
-
2006
- 2006-07-12 CN CNB2006100129427A patent/CN100493336C/en not_active Expired - Fee Related
Non-Patent Citations (6)
Title |
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Ca2+浓度对黔灵山喀斯特生境中几种苔藓植物生长的影响. 陈蓉蓉等.贵州师范大学学报(自然科学版),第16卷第1期. 1998 |
Ca2+浓度对黔灵山喀斯特生境中几种苔藓植物生长的影响. 陈蓉蓉等.贵州师范大学学报(自然科学版),第16卷第1期. 1998 * |
pH值对黔灵山喀斯特生境中几种苔藓植物生长的影响. 陈蓉蓉等.贵州环保科技,第4卷第1期. 1998 |
pH值对黔灵山喀斯特生境中几种苔藓植物生长的影响. 陈蓉蓉等.贵州环保科技,第4卷第1期. 1998 * |
甘肃省小陇山林区药用苔藓植物初报. 杨亚军等.天水师范学院学报,第26卷第2期. 2006 |
甘肃省小陇山林区药用苔藓植物初报. 杨亚军等.天水师范学院学报,第26卷第2期. 2006 * |
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CN1887066A (en) | 2007-01-03 |
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Granted publication date: 20090603 Termination date: 20120712 |