CN104206147A - Novel cultivation method for butterfly orchid tissue culture seedlings - Google Patents
Novel cultivation method for butterfly orchid tissue culture seedlings Download PDFInfo
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- CN104206147A CN104206147A CN201410412551.9A CN201410412551A CN104206147A CN 104206147 A CN104206147 A CN 104206147A CN 201410412551 A CN201410412551 A CN 201410412551A CN 104206147 A CN104206147 A CN 104206147A
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Abstract
The invention relates to a novel cultivation method for butterfly orchid tissue culture seedlings, and belongs to the technical field of flower culture, particularly orchid cultivation. The novel cultivation method comprises the following steps: (1), preparing a cultivation medium: mixing coconut fiber and volcanic rock after being processed to prepare a cultivation medium, or mixing the coconut fiber and float grass imported from Chile, after being processed, to prepare the cultivation medium, or mixing the volcanic rock and the float grass imported from Chile, after being processed, to prepare the cultivation medium, or mixing the coconut fiber, the volcanic rock and the float grass imported from Chile, after being processed, to prepared the cultivation medium, or preparing the coconut fiber after being processed into the cultivation medium; (2), getting a culture flask for loading butterfly orchid tissue culture seedlings, taking the butterfly orchid tissue culture seedlings out of the culture flask, cutting off dead roots and yellow leaves, washing root systems to be clean, and obtaining butterfly orchid tissue culture seedlings to be transplanted; the cultivation medium adopted in the invention has the advantages that degradation products are free from contamination, and good water holding, ventilating, moisture-holding, fertilizer retaining functions are achieved, and the requirements of the growth of the butterfly orchid are met; moreover, the denudation of microbes can be resisted, the probability of rottenness is low, and the cost is low.
Description
Technical field
The present invention relates to a kind of new method for cultivating of Moth orchid plantlet in vitro.Belong to floriculture particularly orchid technical field of cultivation.
Background technology
Moth orchid (Phalaenopsis B1.Spp.) is spent greatly because of it, and the florescence is long, and pattern is gorgeous, color and luster enriches, and flower shape is beautiful unique, as butterfly dances lightly, thus gaining the name, have the laudatory title of " orchid queen " in hot bandwidth, is high-grade indoor pot flowers popular on China flowers market.Current majority adopts the method for tissue cultures to carry out Fast-propagation to Moth orchid.
Moth orchid plantlet in vitro be at enclosed sterile, nutrition supply is abundant, illumination is suitable for, grow in the environment of temperature constant and 100% relative moisture, once bottle outlet is transplanted, environment is unfavorable for its acute variation grown, due to test-tube plantlet tissue, children is tender, poor to new adaptive capacity to environment, very easily by infection process.Test-tube seedling transplanting survival rate, except affecting by factors such as illumination, temperature, humidity, sick worm prevention and control, nutrition supplies, also by the impact of bottle outlet cultivation matrix, bottle outlet cultivation matrix is the key factor affecting test-tube plantlet growth situation and survival rate.The Main Cultivation matrix being Moth orchid with Chilean import pasture and water at present, Chilean import pasture and water can solve the gas permeability problem of cultivation matrix preferably, use as industrialization in Moth orchid industry, large-scale cultivation substrate.But along with planting time increases, there will be acidifying and the phenomenon that addles with the cultivation matrix that Chilean import pasture and water are formed merely, easily cause saprophytic bacteria grow; In addition, developing rapidly in recent years due to Moth orchid industry supply and demand amount, to the demand also rapid development of cultivation matrix pasture and water, on market pasture and water supply falls short of demand.The more important thing is the import of described Chilean import pasture and water long, price one tunnel is high, such as: 2012 the end of the years 3.2 ten thousand yuan per ton of Chile import pasture and water, within 2013, then rise to 3.5 ten thousand yuan to 3.9 ten thousand yuan per ton.Rising on the way of cultivation matrix price, directly makes Moth orchid individual plant seedling cost price go up.To sum up reason, the trend of Moth orchid industry development substitutes expensive Chilean import pasture and water matrix in the urgent need to finding a kind of Moth orchid growth, easily operation and lower-cost material of being more applicable to.
Summary of the invention
Object of the present invention, be for easily there is matrix acidifying in the cultivation of existing Moth orchid plantlet in vitro and addle, problem that cost is high, a kind of new method for cultivating of Moth orchid plantlet in vitro is provided, has that cost is low, transplanting survival rate is high, seedling strain is healthy and strong and be convenient to the advantages such as group basin.
Object of the present invention can take following technical scheme to reach:
A new method for cultivating for Moth orchid plantlet in vitro, is characterized in that:
1) cultivation matrix is prepared
Cultivation matrix is mixed and made into by after coconut palm monofilament and volcanic rock process, or be mixed and made into cultivation matrix by after coconut palm monofilament and the process of Chilean import pasture and water, or be mixed and made into cultivation matrix by after volcanic rock and the process of Chilean import pasture and water, or be mixed and made into cultivation matrix by after coconut palm monofilament, volcanic rock and the process of Chilean import pasture and water; Or cultivation matrix is made in the process of coconut palm monofilament;
2) be ready to cultivate bottled Moth orchid plantlet in vitro
From blake bottle, Moth orchid plantlet in vitro is taken out, wipe out the yellow leaf of dead root, rinse root system well, obtain the Moth orchid plantlet in vitro of transplanting training;
3) by step 1) described in cultivation matrix parcel step 2) the root system of Moth orchid plantlet in vitro, be planted in transparent nutrition cup;
4) by step 3) Moth orchid plantlet in vitro move in booth and cultivate, cultivation condition is: temperature 20-28 DEG C, intensity of illumination 4000-8000lx, relative air humidity 70-90%.
Object of the present invention can also take following technical scheme to reach:
A kind of preferred embodiment is, after Moth orchid plantlet in vitro moves into booth, temperature 20-28 DEG C, intensity of illumination 4000-8000lx, relative air humidity 70-90% cultivation condition under, cultivate 3 months.Described nutrition cup is 1.5 cun of transparent nutrition cups.
Further preferred embodiment is:
Cultivation matrix is mixed after treatment by coconut palm monofilament and Chilean import pasture and water, and the volume ratio of each material is: coconut palm monofilament: Chilean import pasture and water=0.5-1:0.5-1; First the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinse well, wring out, soak tpn thimerosal again 30 minutes, the described Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, then rinses well, wring out, then soak tpn thimerosal 30 minutes; By volume coconut palm monofilament and Chilean import pasture and water mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
Or cultivation matrix is mixed by coconut palm monofilament and volcanic rock and forms, and the volume ratio of each material is: coconut palm monofilament: volcanic rock=1:1; First the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinse well, wring out, soak tpn thimerosal again 30 minutes, by volume coconut palm monofilament and volcanic rock mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
Or cultivation matrix is made up of volcanic rock and the hybrid combining of Chilean import pasture and water, and the volume ratio of each material is: volcanic rock: Chilean import pasture and water=1:1; First the described Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, then rinse well, wring out, then soak tpn thimerosal 30 minutes; By volume volcanic rock and Chilean import pasture and water mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
Further preferred embodiment is, cultivation matrix is mixed by coconut palm monofilament, volcanic rock and Chilean import pasture and water and forms; The volume ratio of each material is: coconut palm monofilament: volcanic rock: Chilean import pasture and water=1:1:1; First the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinse well, wring out, soak tpn thimerosal again 30 minutes, the described Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, then rinses well, wring out, then soak tpn thimerosal 30 minutes; By volume coconut palm monofilament, volcanic rock and Chilean import pasture and water mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
A kind of simplified embodiment is that cultivation matrix is made up of coconut palm monofilament, first the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinses well, wring out, then soak tpn thimerosal 30 minutes, make cultivation matrix; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
A kind of contrast embodiment is, cultivation matrix is made up of Chilean import pasture and water, the described Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, then rinse well, wring out, then soak tpn thimerosal 30 minutes, make cultivation matrix, then this with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
Further, described tpn thimerosal is 1000 times of tpns of routine techniques.
The present invention has following outstanding beneficial effect:
1, the cultivation matrix of the present invention's employing, form owing to adopting coconut palm monofilament or adopt coconut palm monofilament as the key component of composite cultivation substrate, therefore, there is following aspect effect: the functions such as (1) degradation product is pollution-free, good water conservation, ventilative, moisturizing, fertilizer conservation, meet Moth orchid growth requirement; (2) antimicrobial degrading can be supported, not perishable, can not pass along with planting time and occur acidifying and the phenomenon that addles, the growth of Moth orchid plantlet in vitro can be promoted; (3) consumption of import pasture and water is reduced, cost-saving.
2, the cultivation matrix of the present invention's employing, form owing to adopting volcanic rock or adopt volcanic rock as the key component of composite cultivation substrate, cultivation matrix is made to have loose structure, there is following aspect effect: (1) medium density, loose and gas permeability, water binding capacity and drainability are all good, WGR rationally, be applicable to the features such as blue root growth, (2) to the plantlet in vitro of transplanting water pour water in and the harmful substance that gives off of blue root have very strong adsorptivity and permutation function; (3) containing macronutrient and several mineral materials trace element, and there is slow-release function, the growth of Moth orchid plantlet in vitro can be promoted; (4) consumption of import pasture and water is reduced, cost-saving.
3, the cultivation matrix that adopts of the present invention, carry out mixing owing to adopting coconut palm monofilament and volcanic rock by (volume ratio) and form, there is good water conservation, breathe freely, moisturizing, the function such as fertilizer conservation, meet the growth requirement of Moth orchid.Compared with traditional import pasture and water matrix, also have adsorb harmful components, corrosion-resistant, be applicable to the advantages such as long-time cultivation.Reduce the consumption of import pasture and water, cost-saving.
4, the composite interstitial substance of the present invention's employing, because its base substance component comprises coconut palm palm fibre, or comprise coconut palm monofilament and volcanic rock, therefore, relative to the simple matrix with Chilean import pasture and water composition, have anti-acidifying, rot-proof, prevent saprophytic bacteria from growing and medium density, the feature such as loose, gas permeability, water binding capacity are good and drainability is good, the WGR making Moth orchid plantlet in vitro root rationally, is conducive to growing of blue root.The dependence to Chilean import pasture and water can be reduced, have that cost is low, twice laid, protection of the environment and beneficial effect easy to utilize.
5, when the present invention is owing to transplanting plantlet in vitro, adopt the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup, then Moth orchid plantlet in vitro is moved in booth and cultivate, cultivation condition is: temperature 20-28 DEG C, intensity of illumination 4000-8000lx, relative air humidity 70-90%, therefore, not only be conducive to growing of Moth orchid, and saving of work and time, reduce costs, do not affect the group basin work of next stage simultaneously.
6, the present invention is due to when preparing cultivation matrix, soaks and disinfect starting components, therefore, have prevent saprophytic bacteria from growing, anti-acidifying, the feature such as rot-proof, the growth of Moth orchid plantlet in vitro can be promoted.
Embodiment
Specific embodiment:
The new method for cultivating of the Moth orchid plantlet in vitro that the present embodiment relates to, comprises the steps:
1) the material composition of cultivation matrix
Table 1 comprises six kinds of composite cultivation substrates, a kind of simplified cultivation matrix and a kind of material composition contrasting cultivation matrix, composite cultivation substrate is No. 6, matrix No. 1-matrix, simplified cultivation matrix is No. 1, control group, and contrast cultivation matrix is No. 2, control group, shown in the material composition table 1 of each matrix:
The composition of table 1 Moth orchid cultivation matrix and consumption (volume ratio) thereof
| Sample number into spectrum | Coconut palm monofilament | Chile import pasture and water | Volcanic rock |
| No. 1, matrix | 1 | 1 | - |
| No. 2, matrix | 1 | 0.5 | - |
| No. 3, matrix | 0.5 | 1 | - |
| No. 4, matrix | 1 | - | 1 |
| No. 5, matrix | - | 1 | 1 |
| No. 6, matrix | 1 | 1 | 1 |
| No. 1, control group | 1 | - | - |
| No. 2, control group | - | 1 | - |
Note: "-" representative is without the need to adding
2) preparation method of cultivation matrix:
2-1) the coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, rinse well, wring out, then soak 1000 times of tpns 30 minutes;
2-2) coconut palm monofilament is mixed by volume with volcanic rock;
2-3) the Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, wring out, then soak 1000 times of tpns 30 minutes;
2-4) mix according to the proportioning of table 1, obtain the composite interstitial substance of Moth orchid plantlet in vitro cultivation.
Because Moth orchid is aerial root, the gas permeability for cultivation matrix has higher requirement.
3) cultivation method of Moth orchid plantlet in vitro:
3-1) with tweezers, Moth orchid plantlet in vitro is taken out from blake bottle, wipe out the yellow leaf of dead root, rinse the medium of root system well;
3-2) root system composite interstitial substance wraps up, and is planted in 1.5 cun of transparent nutrition cups;
3-3) by step 3-2) Moth orchid plantlet in vitro move in booth and cultivate, cultivation condition is: temperature 20-28 DEG C, intensity of illumination 4000-8000lx, relative air humidity 70-90%.
A kind of preferred embodiment is, after Moth orchid plantlet in vitro moves into booth, temperature 20-28 DEG C, intensity of illumination 4000-8000lx, relative air humidity 70-90% cultivation condition under, cultivate 3 months.Described nutrition cup is 1.5 cun of transparent nutrition cups.
Cultivate after 3 months, to the transplanting survival rate of Moth orchid seedling in each nutrition cup, radical, the newly-increased number of blade, leaf, long and growing way is added up, and result is as shown in table 2 below.
The result of the test of table 2 Moth orchid cultivation
In table 2, the overall merit=transplanting survival rate × radical × newly-increased number of sheets × leaf of cultivation matrix is long
As shown in Table 2, compared with control group, composite interstitial substance of the present invention is used for the cultivation of Moth orchid plantlet in vitro, and the long numerical value of the survival rate of the Moth orchid obtained, radical, the newly-increased number of blade and leaf is all higher, and growing way is good; Particularly No. 2, matrix (coconut palm monofilament: import Chile pasture and water are 0.5:1), radical and the newly-increased number of sheets are best, and plant transplanting seedling time is short, and root of hair is fast, and the vigorous stalwartness of growing way, overall merit is slightly higher than No. 2, contrast (Chilean import pasture and water).From the data of No. 1, contrast, be used alone coconut palm monofilament, also can be used for Moth orchid cultivation, but survival rate is low, growing way is all poor.
In sum; the composite interstitial substance that the present invention relates to; there are the functions such as good water conservation, ventilative, moisturizing, fertilizer conservation; there is anti-acidifying, rot-proof, prevent saprophytic bacteria from growing and medium density, the feature such as loose, gas permeability, water binding capacity are good and drainability is good; meet the growth demand of Moth orchid, be applicable to Moth orchid industrialized scaleization and produce.Not only twice laid ecological, environmental protective, and significantly reduce production cost, make Moth orchid have higher ornamental value, have more market competitiveness.
Claims (8)
1. a new method for cultivating for Moth orchid plantlet in vitro, is characterized in that:
1) cultivation matrix is prepared
Cultivation matrix is mixed and made into by after coconut palm monofilament and volcanic rock process, or be mixed and made into cultivation matrix by after coconut palm monofilament and the process of Chilean import pasture and water, or be mixed and made into cultivation matrix by after volcanic rock and the process of Chilean import pasture and water, or be mixed and made into cultivation matrix by after coconut palm monofilament, volcanic rock and the process of Chilean import pasture and water; Or cultivation matrix is made in the process of coconut palm monofilament;
2) be ready to cultivate bottled Moth orchid plantlet in vitro
From blake bottle, Moth orchid plantlet in vitro is taken out, wipe out the yellow leaf of dead root, rinse root system well, obtain the Moth orchid plantlet in vitro of transplanting training;
3) by step 1) described in cultivation matrix parcel step 2) the root system of Moth orchid plantlet in vitro, be planted in transparent nutrition cup;
4) by step 3) Moth orchid plantlet in vitro move in booth and cultivate, cultivation condition is: temperature 20-28 DEG C, intensity of illumination 4000-8000lx, relative air humidity 70-90%.
2. the new method for cultivating of a kind of Moth orchid plantlet in vitro according to claim 1, it is characterized in that: after Moth orchid plantlet in vitro moves into booth, temperature 20-28 DEG C, intensity of illumination 4000-8000lx, relative air humidity 70-90% cultivation condition under, cultivate 3 months; Described nutrition cup is 1.5 cun of transparent nutrition cups.
3. the new method for cultivating of a kind of Moth orchid plantlet in vitro according to claim 1 and 2, it is characterized in that: cultivation matrix is mixed after treatment by coconut palm monofilament and Chilean import pasture and water, and the volume ratio of each material is: coconut palm monofilament: Chilean import pasture and water=0.5-1:0.5-1; First the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinse well, wring out, soak tpn thimerosal again 30 minutes, the described Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, then rinses well, wring out, then soak tpn thimerosal 30 minutes; By volume coconut palm monofilament and Chilean import pasture and water mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
4. the new method for cultivating of a kind of Moth orchid plantlet in vitro according to claim 1 and 2, is characterized in that: cultivation matrix is mixed by coconut palm monofilament and volcanic rock and forms, and the volume ratio of each material is: coconut palm monofilament: volcanic rock=1:1; First the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinse well, wring out, soak tpn thimerosal again 30 minutes, by volume coconut palm monofilament and volcanic rock mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
5. the new method for cultivating of a kind of Moth orchid plantlet in vitro according to claim 1 and 2, is characterized in that: cultivation matrix is made up of volcanic rock and the hybrid combining of Chilean import pasture and water, and the volume ratio of each material is: volcanic rock: Chilean import pasture and water=1:1; First the described Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, then rinse well, wring out, then soak tpn thimerosal 30 minutes; By volume volcanic rock and Chilean import pasture and water mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
6. the new method for cultivating of a kind of Moth orchid plantlet in vitro according to claim 1 and 2, is characterized in that: cultivation matrix is mixed by coconut palm monofilament, volcanic rock and Chilean import pasture and water and forms; The volume ratio of each material is: coconut palm monofilament: volcanic rock: Chilean import pasture and water=1:1:1; First the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinse well, wring out, soak tpn thimerosal again 30 minutes, the described Chilean import pasture and water running water of 1.5 times of volumes is soaked 4 hours, then rinses well, wring out, then soak tpn thimerosal 30 minutes; By volume coconut palm monofilament, volcanic rock and Chilean import pasture and water mix by ratio again, make cultivation matrix, and this cultivation matrix is composite cultivation substrate; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
7. the new method for cultivating of a kind of Moth orchid plantlet in vitro according to claim 1 and 2, it is characterized in that: cultivation matrix is made up of coconut palm monofilament, first the described coconut palm monofilament running water of 1.5 times of volumes is soaked 2-3 days, then rinse well, wring out, soak tpn thimerosal again 30 minutes, make cultivation matrix; Then with the root system of cultivation matrix parcel Moth orchid plantlet in vitro, be planted in transparent nutrition cup.
8. the new method for cultivating of a kind of Moth orchid plantlet in vitro according to claim 1 and 2, is characterized in that: described tpn thimerosal is 1000 times of tpns of routine techniques.
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| CN108450275A (en) * | 2018-01-20 | 2018-08-28 | 厦门和鸣花卉科技有限公司 | In iris seedling to Flowering Plants breeding method |
| CN109662010A (en) * | 2019-02-14 | 2019-04-23 | 芜湖东源新农村开发股份有限公司 | It is a kind of to prevent iris rotten culture substrate and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993879A (en) * | 2016-05-23 | 2016-10-12 | 云南创农农业科技有限公司 | Crop cultivation substrate, and preparation method and application thereof |
| CN108450275A (en) * | 2018-01-20 | 2018-08-28 | 厦门和鸣花卉科技有限公司 | In iris seedling to Flowering Plants breeding method |
| CN109662010A (en) * | 2019-02-14 | 2019-04-23 | 芜湖东源新农村开发股份有限公司 | It is a kind of to prevent iris rotten culture substrate and preparation method thereof |
| CN110463556A (en) * | 2019-09-19 | 2019-11-19 | 漳州新镇宇生物科技有限公司 | A kind of iris bottle seedling refinery method |
| CN111492833A (en) * | 2020-04-15 | 2020-08-07 | 南京凝绿生物科技有限公司 | Agarose gel matrix cultivated chlorophytum comosum potted plant product and preparation method thereof |
| CN111528071A (en) * | 2020-04-15 | 2020-08-14 | 南京凝绿生物科技有限公司 | Roughhaired lemongrass potted plant cultivated by agarose gel matrix and preparation method thereof |
| CN111593748A (en) * | 2020-06-02 | 2020-08-28 | 浙江理工大学 | Ecological protection structure of broken rock abrupt slope and construction method thereof |
| CN112314380A (en) * | 2020-11-12 | 2021-02-05 | 沭阳呈祥花卉研究开发有限公司 | Cultivation method of good-growth phalaenopsis |
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