CN106134949A

CN106134949A - A kind of greening flowers cultivation matrix

Info

Publication number: CN106134949A
Application number: CN201610499851.4A
Authority: CN
Inventors: 李建涛
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-06-28
Filing date: 2016-06-28
Publication date: 2016-11-23

Abstract

The invention discloses a kind of greening flowers cultivation matrix, be prepared by the raw materials in: chicken manure 28 30, Carapax Eriocheir sinensis 10 12, potassium nitrate 68, biphosphate Ca45, chitosan 34, medical stone powder 57, volcanic rock fine powder 56, sunflower plate granulated slag 32 35, Periostracum Serpentis waste material 40 45, pepsin preparation 2.4 2.6,1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochloride 2.2 2.3, N N-Hydroxysuccinimide 0.7 0.8,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate；In the culture matrix of the present invention, Organic substance and trace element enrich, Chicken dung coordinates Carapax Eriocheir sinensis can improve the fertilizer efficiency persistent period, the light weight nutrient matrix raw materials such as volcanic rock fine powder are used while preferably maintaining flowers long term growth, significantly reducing the cost of transportation of substrate, the flowers greening that it is long-acting, measure light feature is allowed to be applicable to overpass, expressway other uses.

Description

A kind of greening flowers cultivation matrix

Technical field

The present invention relates to nutrient matrix technical field, particularly relate to a kind of greening flowers cultivation matrix.

Background technology

Substrate culture is a kind of important cultural method in the current agriculture of China, woods, flower planting industry, is that one passes through base Matter absorbs nutritional solution and a kind of Soilless Culture Methods of oxygen, owing to it is low to soil environment requirement, equipment simple, water-saving saves fertilizer etc. Advantage, is adopted application by vast plant development person.And continuous along with the development of China's plant husbandry and cultivated area Expanding, the usage amount of cultivation matrix the most constantly increases, and traditional uses the single raw material such as the peat composed of rotten mosses, Vermiculitum or be simply mixed preparation Substrate can not meet the demand of plantation, and the universal nutrition of present stage substrate simultaneously is single, causes sending out of plant in cultivation Bud rate is low, survival rate is low, the physical instability of substrate during simultaneously using, and water absorption is poor, easily subside, surcharge Low, it is impossible to realize higher using value for specific environment, affect the growth of plantation economy benefit.

Collagen protein, as a kind of biomacromolecule, has good pore structure after being modified, the most raw Thing degradability, mechanical strength and biocompatibility, have good cell growth interface, can become a kind of cultivation matrix and build In quality raw materials.China Serpentis aboundresources simultaneously, owing to the decorative pattern of Periostracum Serpentis outward appearance is the most gorgeous, pattern is unique, is used for making The artware of various finenesses, but due to the backwardness of Periostracum Serpentis Technology, the course of processing creates a large amount of waste material, account for complete The 20-30% of Periostracum Serpentis, if not being effectively treated, not only polluting environment, also wasting substantial amounts of raw material, containing substantial amounts of in Periostracum Serpentis Collagen protein, this also applies for it and provides cheap raw material basis in substrate preparation.

The application for a patent for invention of Publication No. CN1702061A discloses the full nutrient matrix of a kind of botanical seedling culturing and cultivation, This nutrient matrix use weight ratio be the peat of 50-70%, the perlite of 20-25%, the Vermiculitum of 10-15 and active substance, Nutrient substance mixes, and has more comprehensively nutrients structure, but the cultivation matrix of this disclosure of the invention is due to size not One, easily cause sedimentation after watering, cause the blocking of air in substrate, the crude fiber content in substrate is low simultaneously, needs extra battalion Support the interpolation of material, improve production cost, and not for the additional use value of the real needs raising substrate cultivated, limit Make the scale application of product.

Summary of the invention

The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of greening flowers cultivation matrix.

The present invention is achieved by the following technical solutions:

A kind of greening flowers cultivation matrix, is prepared by the raw materials in: chicken manure 28-30, Carapax Eriocheir sinensis 10-12, nitre Acid potassium 6-8, dalcium biphosphate 4-5, chitosan 3-4, medical stone powder 5-7, volcanic rock fine powder 5-6, sunflower plate granulated slag 32-35, Periostracum Serpentis waste material 40-45, pepsin preparation 2.4-2.6,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 2.2- 2.3, N-hydroxy-succinamide 0.7-0.8,4% sodium chloride solution are in right amount, 0.15mol/L citric acid solution is appropriate, water is appropriate.

Described greening flowers specifically comprising the following steps that by cultivation matrix preparation method

(1) chicken manure, Carapax Eriocheir sinensis mixing being placed in fermentation vat, add water to the 66-74% of ferment heap maximum water holding capacity, ferment 23- 27 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, sunflower plate cut off simultaneously, pulverize and be Smalls is standby；

(2) 4% sodium chloride solution is added by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour Centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C, Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min 10-12 minute, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add potassium nitrate, dalcium biphosphate, chitosan, be heated to 54-58 DEG C, reaction After 25-30 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, medical stone powder, volcanic rock fine powder mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, is placed under 4-5 DEG C of low temperature environment, After stirring 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide with And other leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-32 DEG C of drying, obtains substrate of the present invention.

The invention have the advantage that

Substrate of the present invention is by the extraction to Periostracum Serpentis, and by having reduced immunogenicity, the collagen protein of biodegradability should Use in the preparation of cultivation matrix, utilize the mechanical performance of cross-linking modified raising albumen, it is achieved its support in substrate builds Effect, can not only provide certain support and growth interface, the collagenous fiber bundle phase within collagen protein simultaneously for seed seedling Be staggered porous network structure mutually that formed, is also beneficial to beneficial microorganism and carries and the extension of root cell, and is It provides sufficient oxygen and nutrient substance, promotes the growth of plant, and culture matrix plays fixation, convenient transport.

In the culture matrix of the present invention, Organic substance and trace element enrich, and it is lasting that Chicken dung coordinates Carapax Eriocheir sinensis can improve fertilizer efficiency Time, while preferably maintaining flowers long term growth, use the light weight nutrient matrix raw materials such as volcanic rock fine powder, be greatly reduced The cost of transportation of substrate, the flowers greening that it is long-acting, measure light feature is allowed to be applicable to overpass, expressway other uses.

Detailed description of the invention

A kind of greening flowers cultivation matrix, is made up of the raw material of following weight portion (kg): chicken manure 28, Carapax Eriocheir sinensis 10, nitric acid Potassium 6, dalcium biphosphate 4, chitosan 3, medical stone powder 5, volcanic rock fine powder 5, sunflower plate granulated slag 32, Periostracum Serpentis waste material 40, stomach egg White enzyme preparation 2.4,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 2.2, N N-Hydroxysuccinimide 0.7,4% Sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.

(1) chicken manure, Carapax Eriocheir sinensis mixing are placed in fermentation vat, add water to the 66% of ferment heap maximum water holding capacity, ferment 23 days, send out Ferment complete after by fermentation material squeeze and filter, obtain fermentation liquid and ferment slag be standby, sunflower plate is cut off simultaneously, pulverize as smalls standby With；

(2) add 4% sodium chloride solution by 1:16g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6 hours with 5800 revs/min are centrifuged 12 minutes, take precipitation clear water and clean and dry at 30 DEG C, then will precipitation and 0.15mol/L citric acid Solution presses 1:8g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30 DEG C, and insulation is extracted 18 hours, after completing It is heated to 85 DEG C, is incubated 10 minutes, then be centrifuged 10 minutes with 5000 revs/min, collect the supernatant, obtain collagen solution Standby；

(3) in step 1 gained fermentation liquid, add potassium nitrate, dalcium biphosphate, chitosan, be heated to 54 DEG C, react 25 After minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, medical stone powder, volcanic rock fine powder mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4 DEG C of low temperature environments, stir After mixing 5 minutes add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N N-Hydroxysuccinimide and other Leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 30 DEG C of drying, obtains substrate of the present invention.

In order to further illustrate the using value of the present invention, inventor have chosen respectively substrate of the present invention and commercially available commonly Culture matrix carries out the cultivating and growing of Flos Moutan, and during plantation, management method is identical, and during plantation, the physical and chemical index to substrate enters Row detection, result is as follows:

	Full nitrogen (%)	Full phosphorus (%)	Full potassium (%)	Total porosity (%)	Saturation moisture content (%)
						Substrate of the present invention	0.994	0.12	0.43	90.19	900
Commercially available generic media	0.751	0.09	0.40	85.28	481

Substrate the most of the present invention plantation gained Flos Moutan plant height and flower quantity are all significantly increased, it can be seen that the present invention Substrate relatively generic media is the most sufficient, simultaneously stable in physicochemical property, and structure is excellent, has preferable prospect of the application.

Claims

1. a greening flowers cultivation matrix, it is characterised in that be prepared by the raw materials in: chicken manure 28-30, Carapax Eriocheir sinensis 10-12, potassium nitrate 6-8, dalcium biphosphate 4-5, chitosan 3-4, medical stone powder 5-7, volcanic rock fine powder 5-6, sunflower plate powder Slag 32-35, Periostracum Serpentis waste material 40-45, pepsin preparation 2.4-2.6,1-(3-dimethylamino-propyl)-3-ethyl carbodiimide salt Hydrochlorate 2.2-2.3, N-hydroxy-succinamide 0.7-0.8,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, Water is appropriate.

2. according to the greening flowers cultivation matrix described in claims 1, it is characterised in that the concrete steps of preparation method are such as Under:

(1) chicken manure, Carapax Eriocheir sinensis mixing are placed in fermentation vat, add water to the 66-74% of ferment heap maximum water holding capacity, ferment 23-27 days, By fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag be standby, sunflower plate is cut off simultaneously, pulverize as smalls standby With；

(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour with 5800-6200 rev/min is centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C, Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min 10-12 minute, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add potassium nitrate, dalcium biphosphate, chitosan, be heated to 54-58 DEG C, react 25- After 30 minutes cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, medical stone powder, volcanic rock fine powder mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4-5 DEG C of low temperature environment, stirring After 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and its His leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-32 DEG C of drying, obtains substrate of the present invention.