CN105967904A

CN105967904A - Vegetable cultivation medium overcoming geographical limitation

Info

Publication number: CN105967904A
Application number: CN201610494486.8A
Authority: CN
Inventors: 李建涛
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-06-28
Filing date: 2016-06-28
Publication date: 2016-09-28

Abstract

The invention discloses a vegetable cultivation medium overcoming geographical limitation. The vegetable cultivation medium is prepared from, by weight, 23-25 parts of threshed cob, 15-18 parts of tea bran, 5-7 parts of potassium sulfate, 4-5 parts of ammonium phosphate, 2-3 parts of propylene glycol alginate, 4-6 parts of vermiculite powder, 32-33 parts of sunflower disc powder residue, 33-36 parts of snake skin waste, 1.6-2.0 parts of a pepsin preparation, 1.2-1.3 parts of 1-(3-dimethylamino propyl)-3-ethyl carbon diimine hydrochloride salt, 0.3-0.4 part of N-hydroxysuccinimide, a proper amount of 4% sodium chloride solution, a proper amount of 0.15 mol/L citric acid solution and a proper amount of water. The vegetable cultivation medium aims at nutrition needs of vegetables, is reasonable in formulation design, stable in physiochemical property and capable of meeting requirements on normal growing of the vegetables in different cultivation environments; due to flexible adding of raw materials like propylene glycol alginate and tea bran, content of elements easy for the vegetables to absorb and utilize in the medium is increased substantially, and the vegetable cultivation medium is enabled to break geographical limitation, can be used for vegetable cultivation in regions with unsuitable soil conditions and can guarantee stable yield of the vegetables.

Description

A kind of Vegetable culture medium overcoming region to limit

Technical field

The present invention relates to nutrient matrix technical field, particularly relate to a kind of vegetable growing base overcoming region to limit Matter.

Background technology

Substrate culture is a kind of important cultural method in the current agriculture of China, woods, flower planting industry, is a kind of By matrix absorption nutritional solution and a kind of Soilless Culture Methods of oxygen, owing to it requires low, equipment to soil environment Simply, water-saving save the advantages such as fertile, adopted application by vast plant development person.And along with China's plant husbandry Development and the continuous expansion of cultivated area, the usage amount of cultivation matrix the most constantly increases, traditional employing grass The single raw material such as charcoal, Vermiculitum or be simply mixed the substrate of preparation and can not meet the demand of plantation, the most now rank The universal nutrition of Duan Jizhi is single, causes in cultivation that the germination percentage of plant is low, survival rate is low, uses simultaneously The physical instability of journey mesostroma, water absorption is poor, easily subside, surcharge is low, it is impossible to for concrete ring Border realizes higher using value, affects the growth of plantation economy benefit.

Collagen protein, as a kind of biomacromolecule, has good pore structure, preferably after being modified Biological degradability, mechanical strength and biocompatibility, there is good cell growth interface, one can be become Plant the quality raw materials during cultivation matrix builds.China Serpentis aboundresources simultaneously, owing to the decorative pattern of Periostracum Serpentis outward appearance is clear Gorgeous, pattern is unique, is used for making the artware of various fineness, but due to the backwardness of Periostracum Serpentis Technology, The course of processing creates a large amount of waste material, accounts for the 20-30% of complete Periostracum Serpentis, if not being effectively treated, no Only polluting environment, also waste substantial amounts of raw material, containing substantial amounts of collagen protein in Periostracum Serpentis, this also applies for it Substrate preparation provides cheap raw material basis.

The application for a patent for invention of Publication No. CN1702061A discloses the full nutrition of a kind of botanical seedling culturing and cultivation Substrate, this nutrient matrix uses weight ratio to be the peat of 50-70%, the perlite of 20-25%, the Vermiculitum of 10-15 And active substance, nutrient substance mix, there is more comprehensively nutrients structure, but this disclosure of the invention Cultivation matrix differs due to size, easily causes sedimentation after watering, and causes the blocking of air in substrate, simultaneously Crude fiber content in substrate is low, needs the interpolation of extra nutritional material, improves production cost, and does not has pin Real needs to cultivation improve the additional use value of substrate, limit the scale application of product.

Summary of the invention

The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of vegetable overcoming region to limit is planted Training substrate.

The present invention is achieved by the following technical solutions:

A kind of Vegetable culture medium overcoming region to limit, is prepared by the raw materials in: degranulation corncob 23-25, tea bran 15-18, potassium sulfate 5-7, ammonium phosphate 4-5, propylene glycol alginate 2-3, vermiculite power 4-6, Sunflower plate granulated slag 32-33, Periostracum Serpentis waste material 33-36, pepsin preparation 1.6-2.0,1-(3-dimethylamino Propyl group)-3-ethyl-carbodiimide hydrochloride 1.2-1.3, N-hydroxy-succinamide 0.3-0.4,4% sodium chloride Solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.

Specifically comprising the following steps that of the described Vegetable culture medium preparation method overcoming region to limit

(1) degranulation corncob, the mixing of tea bran are placed in fermentation vat, add water to ferment heap maximum water holding capacity 70-73%, ferments 22-24 days, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, Sunflower plate is cut off simultaneously, pulverize as smalls standby；

(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, soak After 6-8 hour centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water clean and in 30-32 DEG C Lower drying, then precipitation is mixed homogeneously by 1:8-10g/mL solid-to-liquid ratio with 0.15mol/L citric acid solution, add Entering pepsin preparation, be heated to 30-32 DEG C, insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, it is incubated 10 minutes, more centrifugal 15-18 minute with 5000-5500 rev/min, collect the supernatant, Obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add potassium sulfate, ammonium phosphate, propylene glycol alginate, be heated to 56-60 DEG C, after reacting 30-32 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, Vermiculitum Powder mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, be placed in 4-5 DEG C low Temperature environment under, after stir 5-10 minute addition 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and other leftover materials, cross-linking reaction 8-10 hour, heats up material after completing To 30-35 DEG C of drying, obtain substrate of the present invention.

The invention have the advantage that

Substrate of the present invention, by the extraction to Periostracum Serpentis, will have the collagen egg of reduced immunogenicity, biodegradability It is applied in vain in the preparation of cultivation matrix, utilizes the mechanical performance of cross-linking modified raising albumen, it is achieved it is in substrate Support effect in structure, can not only provide certain support and growth interface, collagen egg simultaneously for seed seedling The porous network structure that the interlaced arrangement of white internal collagenous fiber bundle is formed, is also beneficial to beneficial microorganism Carry and the extension of root cell, and provide sufficient oxygen and nutrient substance for it, promote the growth of plant, And culture matrix is played fixation, convenient transport.

The culture matrix of the present invention is for the nutrient demand of vegetable, and formula is reasonable in design and physicochemical properties are steady Fixed, it is possible to be suitable for vegetable normal growth in different planting environments, the raw material such as propylene glycol alginate, tea bran Add flexibly, significantly improved the constituent content that vegetable in substrate easily absorbs, enable to break region limit System, carries out the cultivation of vegetable in the unaccommodated area of edaphic condition and ensures the yield that vegetable is stable.

Detailed description of the invention

A kind of Vegetable culture medium overcoming region to limit, is made up of the raw material of following weight portion (kg): degranulation Corncob 23, tea bran 15, potassium sulfate 5, ammonium phosphate 4, propylene glycol alginate 2, vermiculite power 4, Helianthi Dish granulated slag 32, Periostracum Serpentis waste material 33, pepsin preparation 1.6,1-(3-dimethylamino-propyl)-3-ethyl carbon two Inferior amine salt hydrochlorate 1.2, N N-Hydroxysuccinimide 0.3,4% sodium chloride solution are appropriate, 0.15mol/L citric acid Solution is appropriate, water is appropriate.

(1) degranulation corncob, the mixing of tea bran are placed in fermentation vat, add water to the 70% of ferment heap maximum water holding capacity, Ferment 22 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, simultaneously will be to day Certain herbaceous plants with big flowers dish cuts off, it is standby to pulverize as smalls；

(2) add 4% sodium chloride solution by 1:16g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, soak 6 little It is centrifuged 12 minutes with 5800 revs/min time after, takes precipitation clear water and clean and dry at 30 DEG C, then will precipitation Mix homogeneously by 1:8g/mL solid-to-liquid ratio with 0.15mol/L citric acid solution, add pepsin preparation, add Heat is to 30 DEG C, and insulation is extracted 18 hours, completes post-heating and is warming up to 85 DEG C, is incubated 10 minutes, then with 5000 Revs/min centrifugal 15 minutes, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add potassium sulfate, ammonium phosphate, propylene glycol alginate, be heated to 56 DEG C, cool down after reacting 30 minutes, then mix with step 1 gained ferment slag, sunflower plate granulated slag, vermiculite power Standby；

(4) step 2 gained collagen solution is joined in step 3 gained material, be placed in 4 DEG C of low temperature Under environment, after stirring 5 minutes, add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N hydroxyl Base butanimide and other leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 30 DEG C Dry, obtain substrate of the present invention.

In order to further illustrate the using value of the present invention, inventor have chosen substrate of the present invention and commercially available respectively Nostoc commune Vanch substrate carries out the cultivating and growing of vegetable, and during plantation, management method is identical, to substrate during plantation Physical and chemical index detects, and result is as follows:

	Full nitrogen (%)	Full phosphorus (%)	Full potassium (%)	Total porosity (%)	Saturation moisture content (%)
						Substrate of the present invention	1.039	0.10	0.42	90.11	834
Commercially available generic media	0.773	0.09	0.37	85.45	446

Substrate the most of the present invention plantation gained yield of vegetables relatively generic media improves more than 10%, it can be seen that the present invention Substrate relatively generic media is the most sufficient, simultaneously stable in physicochemical property, and structure is excellent, has preferably Prospect of the application.

Claims

1. the Vegetable culture medium overcoming region to limit, it is characterized in that, be prepared by the raw materials in: degranulation corncob 23-25, tea bran 15-18, potassium sulfate 5-7, ammonium phosphate 4-5, propylene glycol alginate 2-3, vermiculite power 4-6, sunflower plate granulated slag 32-33, Periostracum Serpentis waste material 33-36, pepsin preparation 1.6-2.0,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.2-1.3, N-hydroxy-succinamide 0.3-0.4,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.

2. according to overcoming, described in claims 1, the Vegetable culture medium that region limits, it is characterised in that specifically comprising the following steps that of preparation method

(1) degranulation corncob, the mixing of tea bran are placed in fermentation vat, add water to the 70-73% of ferment heap maximum water holding capacity, ferment 22-24 days, by fermentation material squeeze and filter after having fermented, fermentation liquid and ferment slag standby, sunflower plate is cut off simultaneously, pulverize as smalls standby；

(2) 4% sodium chloride solution is added by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water to clean and dry at 30-32 DEG C, again precipitation is mixed homogeneously by 1:8-10g/mL solid-to-liquid ratio with 0.15mol/L citric acid solution, add pepsin preparation, it is heated to 30-32 DEG C, insulation is extracted 18-20 hour, complete post-heating and be warming up to 85-95 DEG C, it is incubated 10 minutes, centrifugal 15-18 minute with 5000-5500 rev/min again, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid add potassium sulfate, ammonium phosphate, propylene glycol alginate, be heated to 56-60 DEG C, after react 30-32 minute cooling, then with step 1 gained ferment slag, sunflower plate granulated slag, vermiculite power mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, it is placed under 4-5 DEG C of low temperature environment, 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and other leftover materials are added after stirring 5-10 minute, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of drying, obtains substrate of the present invention.