CN106106103A - Scribbling sketch Herba Dendrobii old stem cuttage breeding method - Google Patents
Scribbling sketch Herba Dendrobii old stem cuttage breeding method Download PDFInfo
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- CN106106103A CN106106103A CN201610540801.6A CN201610540801A CN106106103A CN 106106103 A CN106106103 A CN 106106103A CN 201610540801 A CN201610540801 A CN 201610540801A CN 106106103 A CN106106103 A CN 106106103A
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- 238000009395 breeding Methods 0.000 title claims abstract description 26
- 230000008641 drought stress Effects 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 6
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 4
- 238000003786 synthesis reaction Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000003337 fertilizer Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 239000004575 stone Substances 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 8
- 239000003610 charcoal Substances 0.000 claims description 7
- 201000004384 Alopecia Diseases 0.000 claims description 5
- 241001494479 Pecora Species 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 5
- 230000003676 hair loss Effects 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 230000003442 weekly effect Effects 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 238000002054 transplantation Methods 0.000 abstract description 6
- 230000032683 aging Effects 0.000 abstract description 5
- 238000010899 nucleation Methods 0.000 abstract description 5
- 235000006286 nutrient intake Nutrition 0.000 abstract description 2
- 230000007423 decrease Effects 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 230000006698 induction Effects 0.000 description 8
- 230000004083 survival effect Effects 0.000 description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 4
- 241000233855 Orchidaceae Species 0.000 description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 4
- 239000006013 carbendazim Substances 0.000 description 4
- 241001523681 Dendrobium Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 125000003636 chemical group Chemical group 0.000 description 3
- 201000009442 piebaldism Diseases 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- 241001104043 Syringa Species 0.000 description 2
- 235000004338 Syringa vulgaris Nutrition 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008654 plant damage Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of scribbling sketch Herba Dendrobii old stem cuttage breeding method, with 2 years or perennial scribbling sketch Herba Dendrobii pseudobulb as material, utilize hot and humid under the conditions of, carry out drought stress, induced synthesis sprouting on old stem, again by final-period management and little transplantation of seedlings, cultivate and there is the new plant keeping parent's merit.The application present invention can obtain the cuttage seedling keeping parental trait completely, 3～seedling in May, 10～December i.e. bloom, stem section sprouting 12～sprouting in 25 days, each pseudobulb 1～4 bud, germination rate more than 90%, compared with aseptic seeding breeding and tissue culture propagation, there is the features such as inheritance stability, cultivation period is short, become to spend soon, production cost is low, simple to operate, easy to spread, aging pseudobulb can be effectively utilized simultaneously and cultivate healthy and strong nascent plant, reduce the decline pseudobulb nutrient consumption to newborn pseudobulb, extend seedling service life.Therefore, the present invention effectively utilizes provide a new way for scribbling sketch Herba Dendrobii seedling breeding and old stem.
Description
Technical field
The invention belongs to Herba Dendrobii vegetative propagation technique field of scribbling sketch, particularly relate to a kind of scribbling sketch Herba Dendrobii old stem cottage propagation side
Method.
Background technology
Herba Dendrobii (Dendrobium.Compactum White " Mutation ") of " scribbling sketch " is the stone in recent years introduced from Thailand
Dry measure used in former times orchid cenospecies, belongs to Depositum urinae preparatum dry measure used in former times kind, leaf oblong, and green, petal and sepal are white, its came purple piebaldism,
Lip lilac band white stripes, spends several 10～30, Hua Jing 6～8cm, plant height 20～30cm, and the natural florescence is May and JIUYUE, group
Body florescence 45～60 days.Herba Dendrobii pattern of " scribbling sketch " is special, and the florescence is long, and flower amount is big, is deeply liked by popular and orchid friend, currently can not be
The fine work potted orchids types obtained more.It is short that Herba Dendrobii of " scribbling sketch " introduces a fine variety the time in China, and relevant study on reproduction report is few, current stone
The breeding of dry measure used in former times orchid is main uses aseptic seeding breeding, tissue culture propagation and division propagation etc..Herba Dendrobii aseptic seeding of " scribbling sketch " breeding requirement
Certain aseptic culture condition, operation complexity, and filial generation separate serious, it is difficult to the filial generation seedling that acquired character is neat.Tissue training
Support set up group culturation rapid propagating technology system need time long, with high content of technology, flower-grower is difficult to grasp, and is unfavorable for Technique Popularizing.Point
Strain breeding is easily caused accumulation and the propagation of virus, causes the reduction of ornamental quality.Meanwhile, " scribble sketch " Herba Dendrobii aseptic seeding and tissue
Cultivation easily causes filial generation reversion, it is thus achieved that spend filial generation in vain, makes ornamental values and the economic values be remarkably decreased.Additionally, Herba Dendrobii without
Bacterium seed propagation, tissue culture propagation and division propagation, the stem apex of the most raw plant of propagating materials collection, stem section, bennet, pseudobulb with
And the seed etc. gathered in the crops then, aging pseudobulb directly abandons, and seldom utilizes old stem to carry out seedling breeding.Gather and give birth to device then
Official is serious to plant injury and nutrient consumption, and the parent that setting up fast breeding technique system needs measures greatly.
Summary of the invention
The technical problem to be solved in the present invention be to provide a kind of simple to operate, production cost is low, cultivation period is short, it is fast to become to spend
And the scribbling sketch Herba Dendrobii old stem cuttage breeding method of inheritance stability.
For solve above-mentioned technical problem, the present invention by the following technical solutions:
Scribbling sketch Herba Dendrobii old stem cuttage breeding method, with 2 years or perennial scribbling sketch Herba Dendrobii pseudobulb as material, utilizes high temperature high
Under the conditions of wet, carry out drought stress, induced synthesis sprouting on old stem, then by final-period management and little transplantation of seedlings, cultivation has
Keep the new plant of parent's merit.
Above-mentioned scribbling sketch Herba Dendrobii old stem cuttage breeding method, including the collection of<1>old stem, the process of<2>stem section,<3>stem segment cuttage,
<4>management,<5>seedling transplant step after inserting.
Step<1>is carried out by following operation: select life in 2 years to the most perennial, baldness, without leaf, the old stem of shrinkage, cut with shears
Lower as cutting, stem segment length is 5～8cm.
Step<2>is carried out by following operation: cut flat by old stem inferior end notch, and inferior end notch is away from stipes 0.5cm, stem segment length
Being 5～8cm, otch is coated with 75% carbendazim powder to be stained with.
Step<3>is carried out by following operation: be placed in cultivation torr using the mixing of bark, stone and Linesless charcoal as cutting medium,
The old stem handled well is lain on substrate face.
Step<4>is carried out by following operation: within after cuttage 10～20 days, stops watering, carries out stem section sprouting by drought stress
Induction.
Step<5>is carried out by following operation: height of seedling length to 6～8cm, root length 3～5cm, is taken off by seedling with shears and carries out
Transplanting, transplanting medium uses Petiolus Trachycarpi clothing parcel root, is placed in mouthful stem 7cm air-and water-permeable plastic cup and cultivates.
In step<1>old stem acquisition time select spring temperature get warm again after a cold spell after or autumn in summer hot weather.
In step<3>, cutting medium is 2: 1: 1 mixing by bark, stone and three kinds of matrix ratios of Linesless charcoal.
In step<4>after stem section sprouting sprouts, recover water and fertilizer management, autumn in summer high temperature season 1～water for 2 days 1 time, keep
Air humidity 70～80%；Executing weekly the sheep stool water fermented 1 time, execute the more No. 10 fertilizer of 1 flower, both alternately use.
For the problem that the breeding of scribbling sketch Herba Dendrobii exists at present, inventor establishes a kind of scribbling sketch Herba Dendrobii old stem cottage propagation side
Method, with 2 years or perennial scribbling sketch Herba Dendrobii pseudobulb as material, utilize hot and humid under the conditions of, carry out drought stress, on old stem
Induced synthesis sprouting, then by final-period management and little transplantation of seedlings, cultivate and there is the new plant keeping parent's merit.With now
Technology is compared, and the present invention has an advantage highlighted below:
(1) make full use of the old stem of " scribbling sketch " Herba Dendrobii, open the new way of " scribbling sketch " Herba Dendrobii sapling multiplication." scribble sketch " Herba Dendrobii
After pseudobulb is aging, along with gradually using up of nutrient, false squama becomes thin and weak, the most withered and the most yellow, and last whole strain is dead.Therefore, Herba Dendrobii
Aging dendrobium plant is typically directly abandoned by blue plantation operator, rarely has and utilizes aging pseudobulb to carry out sapling multiplication, this
Bright is effectively to utilize the old stem of " scribbling sketch " Herba Dendrobii, cultivates the filial generation keeping parent's merit completely, it is provided that breed easily
Means.
(2) the new plant obtained keeps the merit of parent, becomes to spend fast, and breeding cycle is short, and production cost is low.Pass through
The filial generation that Herba Dendrobii old stem of " scribbling sketch " induction sprouting obtains, belongs to asexual propagation, and merit hereditary stability is high, it is not necessary to domestication,
Cultivate through 3～5 months and get final product seedling, 10～can bloom for 12 months；And aseptic seeding growing seedlings and tissue culture propagation, from tissue training
Support to seedling and tame, about 6～10 months seedlings, 18～bloom for 28 months, and there is filial generation atavism.
(3) every stem section induction sprouting number 1～4, old stem inductivity reaches 90～100%, and transplanting survival rate reaches 100%, newly
Bud induction rate and survival rate are high, extend " scribbling sketch " Herba Dendrobii seedling service life, reduce production cost.
(4) simple to operate, production cost is low, it is easy to promote in company and peasant household.
Detailed description of the invention
Embodiment 1
<1>old stem collection
In August, 2014, select life in 2 years, baldness, without leaf, 20, the old stem of slightly shrinkage, cut as cutting, stem with shears
Segment length is 5～8cm.
Old stem acquisition time preferably select spring temperature get warm again after a cold spell after or autumn in summer hot weather, winter, temperature was low, and sprouting is difficult to lure
Lead.
<2>stem section processes
Being cut flat by old stem inferior end notch end, inferior end notch is away from stipes about 0.5cm, and stem segment length is 5～8cm, otch with
75% carbendazim powder is coated with to be stained with.
<3>stem segment cuttage
Using bark, stone and Linesless charcoal three kinds of substrate (a diameter of 2～3cm) ratio be 2: 1: 1 mixing as cutting medium, put
In cultivating in torr, the old stem handled well is lain on substrate face.
<4>manage after inserting
Within after cuttage 10～20 days, stop watering, carry out the new bud inducement of stem section by drought stress.After stem section sprouting sprouts,
Recover water and fertilizer management, depending on weather condition watering, fertilizing, autumn high temperature season 1～water 1 time for 2 days (to water after 4 o'clock of afternoon
Being advisable, the rainy day does not waters), keep air humidity 70～80%；Execute weekly the sheep stool water (concentration is 0.2%) fermented 1 time, execute
U.S. SCOTTS the more No. 10 fertilizer (N: P: K=3: 1: 1, concentration is 0.1%) of flower of 1 Israel's Chemical Group production, two
Person alternately uses；After 15 days, sprouting starts to sprout, and every stem section sprouting number is 1～3, and new bud induction rate is 100%, after 2 months
The high about 4cm of sprouting.
<5>little transplantation of seedlings
Height of seedling length to 6～8cm, root length 3～5cm, with shears seedling taken off and transplants, transplanting medium uses Petiolus Trachycarpi clothing
Parcel root, is placed in mouthful stem 7cm air-and water-permeable plastic cup and cultivates, and transplanting survival rate reaches 100%, can open after 12 months
Flower.
Embodiment 2
<1>old stem collection
In July, 2015, select life in 3 years to the most perennial, baldness, without leaf, 20, the old stem in middle and upper part of summary shrinkage, cut with shears
Lower as cutting, stem segment length is 5～8cm.
<2>stem section processes
Being cut flat by old stem inferior end notch end, inferior end notch is away from stipes about 0.5cm, and stem segment length is 5～8cm, otch with
75% carbendazim powder is coated with to be stained with.
<3>stem segment cuttage
Using bark, stone and Linesless charcoal three kinds of substrate (a diameter of 2～3cm) ratio be 2: 1: 1 mixing as cutting medium, put
In cultivating in torr, the old stem handled well is lain on substrate face.
<4>manage after inserting
Within after cuttage 10～20 days, stop watering, carry out the new bud inducement of stem section by drought stress.After stem section sprouting sprouts,
Recover water and fertilizer management, depending on weather condition watering, fertilizing, autumn high temperature season 1～within 2 days, water 1 time (water after 4 o'clock into
Preferably, the rainy day does not waters), keep air humidity 70～80%；Execute weekly the sheep stool water (concentration is 0.2%) fermented 1 time, execute 1
U.S. SCOTTS the more No. 10 fertilizer (N: P: K=3: 1: 1, concentration is 0.1%) of flower that secondary Israel Chemical Group produces, both
Alternately use；After 25 days, sprouting starts to sprout, and every stem section sprouting number is 1～2, and new bud induction rate is 90%, new after 2 months
The high about 4cm of bud.
<5>little transplantation of seedlings
Height of seedling length to 6～8cm, root length 3～5cm, with shears seedling taken off and transplants, transplanting medium uses Petiolus Trachycarpi clothing
Parcel root, is placed in mouthful stem 7cm air-and water-permeable plastic cup and cultivates, and transplanting survival rate reaches 100%, can open after 12 months
Flower.
Embodiment 3
<1>old stem collection
In May, 2016, select life in 2 years, baldness, without leaf, 20, the old stem of slightly shrinkage, cut as cutting, stem with shears
Segment length is 5～8cm.
<2>stem section processes
Being cut flat by old stem inferior end notch end, inferior end notch is away from stipes about 0.5cm, and stem segment length is 5～8cm, otch with
75% carbendazim powder is coated with to be stained with.
<3>stem segment cuttage
Using bark, stone and Linesless charcoal three kinds of substrate (a diameter of 2～3cm) ratio be 2: 1: 1 mixing as cutting medium, put
In cultivating in torr, the old stem handled well is lain on substrate face.
<4>manage after inserting
Within after cuttage 10～20 days, stop watering, carry out the new bud inducement of stem section by drought stress.After stem section sprouting sprouts,
Recover water and fertilizer management, depending on weather condition watering, fertilizing, summer high temperature season 1～water 1 time for 2 days (to water after 4 o'clock of afternoon
Being advisable, the rainy day does not waters), keep air humidity 70～80%；Execute weekly the sheep stool water (concentration is 0.2%) fermented 1 time, execute
U.S. SCOTTS the more No. 10 fertilizer (N: P: K=3: 1: 1, concentration is 0.1%) of flower of 1 Israel's Chemical Group production, two
Person alternately uses；After 12 days, sprouting starts to sprout, and every stem section sprouting number is 1～4, and new bud induction rate is 100%, after 2 months
The high about 4cm of sprouting.
<5>little transplantation of seedlings
Height of seedling length to 6～8cm, root length 3～5cm, with shears seedling taken off and transplants, transplanting medium uses Petiolus Trachycarpi clothing
Parcel root, is placed in mouthful stem 7cm air-and water-permeable plastic cup and cultivates, and transplanting survival rate reaches 100%, can open after 10 months
Flower.
Above-described embodiment result collects such as table 1.
Table 1 all ages and classes " is scribbled sketch " the new bud induction rate of stem of Dendrobium section
The seedling that above-described embodiment obtains is cultivated through 8～10 months, height of seedling 15～20cm, and leaf oblong is green, scape
5～7cm, spend several 5～8, petal and sepal are white, and came purple piebaldism, lip lilac band white stripes, filial generation exists
The aspects such as plant type, leaf color, leaf, pattern and piebaldism are consistent with maternal trait expression, it was demonstrated that application this invention ensures that parent
Merit be stabilized heredity.
Claims (10)
1. a scribbling sketch Herba Dendrobii old stem cuttage breeding method, it is characterised in that with 2 years or perennial scribbling sketch Herba Dendrobii pseudobulb as material
Material, utilize hot and humid under the conditions of, carry out drought stress, induced synthesis sprouting on old stem, then by final-period management and seedling
Transplant, cultivate and there is the new plant keeping parent's merit.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that include the collection of<1>old stem,<
2>stem section process,<3>stem segment cuttage,<4>manage after inserting,<5>seedling transplant step.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that step<1>presses following operation
Carry out: select life in 2 years to the most perennial, baldness, without leaf, the old stem of shrinkage, cut as cutting with shears, stem segment length be 5～
8cm。
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that step<2>presses following operation
Carrying out: cut flat by old stem inferior end notch end, inferior end notch is away from stipes 0.5cm, and stem segment length is 5～8cm, and otch is with bacterium more than 75%
Spirit powder is coated with to be stained with.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that step<3>presses following operation
Carry out: be placed in cultivation torr as cutting medium using the mixing of bark, stone and Linesless charcoal, the old stem handled well is lain in substrate face
On.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that step<4>presses following operation
Carry out: within after cuttage 10～20 days, stop watering, carry out the new bud inducement of stem section by drought stress.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that step<5>presses following operation
Carrying out: height of seedling length to 6～8cm, root length 3～5cm, taken off by seedling with shears and transplant, transplanting medium uses Petiolus Trachycarpi clothing bag
Wrap up in root, be placed in mouthful stem 7cm air-and water-permeable plastic cup and cultivate.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that old stem collection in step<1>
Selection of time summer autumn hot weather.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that cutting medium in step<3>
It is 2: 1: 1 mixing by bark, stone and three kinds of matrix ratios of Linesless charcoal.
Scribbling sketch Herba Dendrobii the most according to claim 1 old stem cuttage breeding method, it is characterised in that step treats stem section in<4>
After sprouting sprouts, recover water and fertilizer management, autumn in summer high temperature season 1～water for 2 days 1 time, keep air humidity 70～80%；Execute weekly
The sheep stool water that 1 time fermented, executes the more No. 10 fertilizer of 1 flower, and both alternately use.
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| CN201610540801.6A CN106106103B (en) | 2016-07-11 | 2016-07-11 | Scribbling sketch dendrobium nobile old stem cuttage breeding method |
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| CN108739321A (en) * | 2018-05-28 | 2018-11-06 | 四川省植物工程研究院 | A kind of wild Dendrodium propagation method |
| CN108935036A (en) * | 2018-05-31 | 2018-12-07 | 安龙县西城秀树农林有限责任公司 | A kind of field nursery propagation method of dendrobium nobile |
| CN111280038A (en) * | 2020-03-04 | 2020-06-16 | 北京林业大学 | Method for quickly obtaining primula denticulata hance old stake seedlings |
| CN114568242A (en) * | 2022-03-15 | 2022-06-03 | 中国热带农业科学院热带作物品种资源研究所 | Efficient plant division propagation method for splash-ink dendrobium |
| CN115176608A (en) * | 2022-06-30 | 2022-10-14 | 贵州省林业科学研究院 | Rapid seedling method for dendrobium officinale stem node induced buds |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106852251A (en) * | 2016-12-27 | 2017-06-16 | 普洱市忠勒佰合农业开发有限公司 | The propagation method of Dendrobium Chrysotoxum Lindl plant division |
| CN108739321A (en) * | 2018-05-28 | 2018-11-06 | 四川省植物工程研究院 | A kind of wild Dendrodium propagation method |
| CN108935036A (en) * | 2018-05-31 | 2018-12-07 | 安龙县西城秀树农林有限责任公司 | A kind of field nursery propagation method of dendrobium nobile |
| CN108935036B (en) * | 2018-05-31 | 2021-06-01 | 安龙县西城秀树农林有限责任公司 | Wild seedling breeding method for dendrobium |
| CN111280038A (en) * | 2020-03-04 | 2020-06-16 | 北京林业大学 | Method for quickly obtaining primula denticulata hance old stake seedlings |
| CN114568242A (en) * | 2022-03-15 | 2022-06-03 | 中国热带农业科学院热带作物品种资源研究所 | Efficient plant division propagation method for splash-ink dendrobium |
| CN114568242B (en) * | 2022-03-15 | 2023-01-13 | 中国热带农业科学院热带作物品种资源研究所 | Efficient plant division propagation method for splash-ink dendrobium |
| CN115176608A (en) * | 2022-06-30 | 2022-10-14 | 贵州省林业科学研究院 | Rapid seedling method for dendrobium officinale stem node induced buds |
| CN115176608B (en) * | 2022-06-30 | 2024-02-13 | 贵州省林业科学研究院 | Rapid seedling method for dendrobium candidum stem node induced buds |
| CN115443819A (en) * | 2022-10-20 | 2022-12-09 | 中国科学院武汉植物园 | Propagation method of Nile's thamnolia |
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Application publication date: 20161116 Assignee: Guangxi Shengjin Agricultural Technology Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION FORESTRY Research Institute Contract record no.: X2023980045763 Denomination of invention: Cutting propagation method of old stem of Dendrobium officinale Granted publication date: 20190716 License type: Common License Record date: 20231106 |