CN114568242B - Efficient plant division propagation method for splash-ink dendrobium - Google Patents
Efficient plant division propagation method for splash-ink dendrobium Download PDFInfo
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Abstract
The invention belongs to the field of plant cultivation, and relates to an efficient plant division propagation method of splash-ink type dendrobium autumn, which comprises the steps of selecting an aged plant, dividing clustered pseudobulbs on the premise of not damaging root necks, carrying out inverted cultivation by taking the pseudobulbs as parent plants, carrying out plant division after sprouts grow out and new roots grow out from root neck buds at the base parts of the pseudobulbs, taking out the pseudobulbs, horizontally placing the pseudobulbs on a flat-bottom tray, carrying out plant division after sprouts grow and new roots grow out from internodes of the pseudobulbs, and carrying out field planting after the grown plants of the new sprouts are managed by normal water fertilizers for 3-6 months. The pseudobulb of the splash-ink dendrobium nobile is taken as a mother plant, measures of inverted cultivation and flat placement moisture preservation are adopted, the apical dominance is broken, the pseudobulb is promoted to sprout and sprout to realize rapid plant division, the plant division time is shortened, the propagation efficiency is improved, batch plant division propagation can be carried out, the production of a large number of plants is realized, and assistance is provided for the industrialized development of the pseudobulb.
Description
Technical Field
The invention belongs to the field of plant cultivation, and relates to a plant division propagation method, in particular to an efficient division propagation method of splash-ink dendrobium.
Background
Dendrobe (Dendrobium spp.) is also called dendrobe, a perennial epiphytic herbaceous plant, is a general name of plants of the genus dendrobe (Dendrobium Swartz, abbreviated as den) of the family Orchidaceae (Orchidaceae), and is called as "four-large cymbidium" in combination with butterfly orchid, cattleya orchid and cymbidium hybridum.
The dendrobium nobile is evergreen dendrobium nobile, and mainly refers to dendrobium nobile groups, dendrobium antelope groups and intercross species of the dendrobium nobile groups and the dendrobium antelope groups. The inflorescence of the dendrobium nobile is extracted from the top of the stem, 10-20 flowers are planted in each inflorescence, the flowering phase is mostly concentrated in autumn, the natural flowering phase is 8-11 months, and the inflorescences are relatively concentrated in 9-10 months. The cut flower has beautiful flower shape, various varieties and long cut flower vase insertion period, is not only an important cut flower variety in the orchid family, but also an important ornamental pot flower, and is considered as the most promising commercial variety of cymbidium continentalis and cymbidium hybridum.
In the filial generation of dendrobium nobile and in the tissue culture propagation, an individual with white flower color and red radial stripes on the edges of petals and a calyx or an individual with red flower color and white radial stripes on the edges of the petals and the calyx can appear, and the red and white coloring positions of the two types of flowers are just opposite. The individual flower color of the dendrobium nobile is very special, the flower color is red and white, the color contrast is large, the dendrobium nobile has layering sense, is deeply loved by orchid lovers, and is called as the dendrobium nobile with "splash ink". The color of the splash-ink dendrobium can be red and white, red and green, purple and white and the like. The probability of the occurrence of the splash-ink dendrobium is about five parts per million, and the quantity is extremely rare, so the market price is high.
Nowadays, the propagation of dendrobium officinale seedlings is generally performed in a tissue culture manner, and a large number of offspring with the same characteristics can be obtained. However, the splash-ink dendrobium nobile is proved to belong to a variety which cannot be propagated by a tissue culture mode after a great number of attempts. Taking the splash-ink dendrobium as an example, wherein the main color of a flower is white, and the edges of petals and a calyx are red, after the offspring plants obtained by tissue culture bloom, the color is not splash-ink type, but is divided into individuals of pure white and pure red; pure white accounts for 70-80% of the total offspring, pure red accounts for 20-30%, and the chance of splash-ink type offspring is still low (about five ten-thousandths). The reason for this is not fully understood at present, and it is generally understood that individuals of the splash-ink type dendrobium are considered to be chimeras, and that color segregation occurs after tissue culture.
At present, the expanding propagation of the splash-ink autumn dendrobium is still in a plant division mode, and the 'splash-ink' character of a progeny plant is more stable and effective. However, as the sprouts germinate slowly, 1 sprout and a few 2 sprouts grow in one year, the time period for plant division is long, the propagation efficiency is very low, and the germination time is uncertain, so that the seedlings are difficult to obtain in batches at the same time, and the industrialization development of the seedlings is restricted.
Disclosure of Invention
The invention aims to provide an efficient plant division propagation method of splash-ink autumn dendrobium aiming at the low plant division propagation efficiency of the existing splash-ink autumn dendrobium, which adopts the inverted cultivation and flat placement mode of pseudobulbs to break the top end advantages, thereby promoting the germination of new buds between a base part and a internode, promoting the rapid germination of the new buds of the pseudobulbs, shortening the plant division time and improving the propagation efficiency.
The technical scheme adopted by the invention is as follows:
an efficient plant division propagation method of splash-ink type dendrobium nobile comprises the stages and measures of inverted cultivation of pseudo bulbs, horizontal placement and moisture preservation of the pseudo bulbs and the like, and comprises the following specific steps:
1. inverted cultivation of pseudobulb
Selecting an aged plant, taking out the whole plant, removing the culture medium wrapping the root, then separating the clustered pseudobulbs on the premise of not damaging the neck of the root, and carrying out inverted cultivation by taking the pseudobulbs as parent plants.
The mature plants are plants that have bloomed or are inflorescence-producing.
The inverted cultivation method comprises the following steps: selecting a strong single pseudobulb, removing all leaves on the stem, penetrating the base part of the pseudobulb upwards from a round hole at the bottom of a nutrition cup and exposing the surface of the nutrition cup, and exposing the top end of the stem downwards from the round hole of the nutrition cup, then wrapping the root neck part with a culture medium, wherein the position of the root neck part is 0.5-1.5 cm lower than the surface of the culture medium, wrapping the culture medium with the nutrition cup and placing the culture medium on an orchid culture bed frame.
2. Division of plants in stage one
Carrying out normal cultivation management except fertilization on the pseudobulb (mother plant) cultivated in an inverted manner, keeping a cultivation medium moist, and cutting off new buds from the mother plant when the root neck bud points at the base part of the pseudobulb grow new buds with the height of 4-6 cm, the root germination number of the new buds reaches more than 5-6 and the root length reaches 2-3 cm; the cut buds are placed in a shade place for 2-3 days, and are wrapped by water moss after the wound is healed, and are temporarily planted in a nutrition cup. And (3) continuously carrying out inverted cultivation on the pseudobulb serving as the mother plant for 2-3 months, and carrying out plant division for 1 time again when the height of the sprout is 4-6 cm, the number of the roots germinated is more than 5-6 and the length is 2-3 cm.
3. Second stage of plant division
Laying a layer of water moss for keeping moisture on the bottom of the tray, taking out the pseudo bulbs cultivated in an inverted manner, removing necrotic parts, and flatly placing the pseudo bulbs on a flat-bottom tray; placing the tray in a greenhouse with a sun shading rate of 40-60%, spraying for 1 time every day, keeping the pseudobulb moist, and cutting off the buds from the pseudobulb when the internodes of the pseudobulb sprout buds with the height of 4-6 cm, the root sprouting number of the buds reaches more than 5-6 and the length reaches 2-3 cm; the cut buds are placed in a shade place for 2-3 days, and are wrapped by water moss after the wound is healed, and are temporarily planted in a nutrition cup.
4. And (3) planting the plant grown by the new bud after normal water and fertilizer management for 3-6 months, and realizing high-efficiency separate propagation of the splash-ink type dendrobium nobile.
The method takes the pseudobulb of the splash-ink dendrobium autumn as a mother plant, adopts the measures of inverted cultivation and flat moisture preservation, breaks the top end advantage, promotes the pseudobulb to sprout and sprout to realize rapid plant division, shortens the plant division time, and improves the propagation efficiency. By adopting the method, after the dendrobium nobile plants are propagated in an early stage and are accumulated to a certain number, batch plant division propagation can be carried out, the production of a large number of plants is realized, and assistance is provided for the industrialized development of the plants.
Detailed Description
The following examples are given to further illustrate embodiments of the present invention. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers.
1. Splash-ink type dendrobium autumn plant division propagation
The first embodiment is as follows: the 'red-white interphase' splash-ink dendrobium (flower color is white, petal and calyx edge have red radial stripes) is used as female parent for plant division propagation.
1. Inverted cultivation of pseudobulb
In the eight bases of research institute of tropical crop variety resources of the institute of agricultural sciences of tropical China in 2020 and 6 months, a mature (flowering) red-white interphase type dendrobium devastating plant is selected, the pedicel is cut off, the whole plant is taken out of a nutrition cup, a culture medium wrapping the root is removed, most of the root is cut off, and then one root of a fasciculate pseudobulb is separated (the root neck of the pseudobulb is not damaged when the root is separated, and a tool such as scissors can be used for assistance when necessary). Selecting a single false bulb with better activity, removing all leaves on the stem, turning the false bulb upside down, enabling the base part of the false bulb to face upwards and penetrate into the round hole at the bottom of the nutrition cup and expose the surface of the nutrition cup, and enabling the top end of the stem to face downwards and expose the outside of the round hole of the nutrition cup; wrapping the root neck part with culture medium such as coconut block, wherein the root neck part is about 1cm lower than the surface of the coconut block, moving the nutrition cup upwards to wrap the coconut block, placing the nutrition cup in an orchid tray, and placing the tray on a greenhouse orchid bedstead.
2. Division of plants in stage one
The pseudobulb cultivated in handstand way is normally cultivated and managed except for fertilization, and the cultivation substrate is kept moist by spraying for 1 time every day according to weather conditions. After the apical dominance is broken, the bud point at the base of the pseudobulb begins to sprout, the volume becomes larger, and the color becomes white after about 1 month. In the 2 nd month period, the bud grows up, turns green, and starts to grow upwards (at this time, the moisture and sufficient sunlight of the culture medium are ensured, so that the new bud grows robustly). By month 3, shoots began to sprout new roots. And when the sprout is about 5cm in height and the sprout number of the new root reaches more than 5-6 and the sprout length reaches 2-3 cm at the 4 th month, the new sprout can be cut off from the stock plant. The cut buds are placed in a shade place for 2-3 days, and are wrapped by water moss after the wound is healed, and are temporarily planted in a 1.5-inch nutrition cup. After the first division, the pseudobulb as the mother plant was further cultivated in an inverted state for 3 months, and the plant was divided 1 time again in accordance with the growth of the sprout (the height of the sprout was about 5cm, the number of the sprouts in the roots reached 5 to 6, and the length reached 2 to 3 cm).
3. Second stage of plant division
Besides the root and neck, internodes on the pseudobulb also have the ability of sprouting. Laying a layer of water moss for keeping moisture on the bottom of the flat-bottom tray, taking out the pseudo-bulb cultivated in an inverted manner from the nutrition cup, or cutting off the pseudo-bulb from the bottom of the nutrition cup, and horizontally placing the pseudo-bulb on the flat-bottom tray after removing the necrotic part. The tray was placed in a greenhouse with 50% sun-shading rate and sprayed 1 time per day to keep the water moss moist with pseudobulbs. Sprouting new buds and sprouting new roots from internodes of the pseudobulb within 4 months, and cutting the new buds from the pseudobulb when the height of the new buds is about 5cm, the root sprouting number of the new buds reaches more than 5-6 and the length reaches 2-3 cm; the cut buds are placed in a shade place for 2-3 days, and are wrapped by water moss after the wound is healed, and are temporarily planted in a nutrition cup.
4. And (4) carrying out normal water and fertilizer management for 4 months on the plant grown by the sprout and then planting the plant to realize the high-efficiency plant division propagation of the splash-ink type dendrobium.
The second embodiment: the 'red-green interphase' splash-ink dendrobium (flower color is red, petals and calyx edge have green radial stripes) is taken as a female parent for plant division propagation.
1. Inverted cultivation of pseudobulb
In the eight bases of research institute of tropical crop variety resources of tropical academy of agricultural sciences in china in 7 months in 2020, a 'red-green interphase type' dendrobium candidum plant which is producing inflorescence is selected, flower stalks are cut off, the whole plant is taken out from a nutrition cup, a culture medium wrapping the roots is removed, most of the roots are cut off, and then one cautious root of a clustered pseudobulb is separated (the root neck of the pseudobulb is not damaged when the root neck is separated, and a tool such as scissors can be used for assistance when necessary). Selecting a single false bulb with good activity, removing all leaves on the stem, turning the false bulb upside down, enabling the base part of the false bulb to face upwards and penetrate through a round hole at the bottom of the nutrition cup and expose the surface of the nutrition cup, and enabling the top end of the stem to face downwards and expose the outside of the round hole of the nutrition cup; wrapping the root neck part with culture medium such as coconut block, wherein the root neck part is about 1cm lower than the surface of the coconut block, moving the nutrition cup upwards to wrap the coconut block, placing the nutrition cup in an orchid tray, and placing the tray on a greenhouse orchid bedstead.
2. Division of plants in stage one
The pseudobulb cultivated in inverted state is subjected to normal cultivation management except fertilization, and is sprayed for 1 time every day according to weather conditions to keep the cultivation substrate moist. After the apical dominance is broken, the bud points at the base of the pseudobulb begin to sprout, the volume becomes larger, and the color begins to turn white after about 1 month. In the 2 nd month period, the bud grows up, turns green, and starts to grow upwards (at this time, the moisture and sufficient sunlight of the culture medium are ensured, so that the new bud grows robustly). By month 3, shoots began to sprout new roots. And when the sprout is about 5cm in height and the sprout number of the new root reaches more than 5-6 and the sprout length reaches 2-3 cm by the 4 th month, the new sprout can be cut from the stock plant. The cut buds are placed in a shade place for 2-3 days, and are wrapped by water moss after the wound is healed, and are temporarily planted in a 1.5-inch nutrition cup. After the first division, the pseudobulb as the mother plant was further cultivated in an inverted state for 3 months, and the plant was divided 1 time again in accordance with the growth of the sprout (the height of the sprout was about 5cm, the number of the sprouts in the roots reached 5 to 6, and the length reached 2 to 3 cm).
3. Second stage of plant division
Besides the root and neck, internodes on the pseudobulb also have the ability of sprouting. Laying a layer of water moss for keeping moisture on the bottom of the flat-bottom tray, taking out the pseudo-bulb cultivated in an inverted manner from the nutrition cup, or cutting off the pseudo-bulb from the bottom of the nutrition cup, and horizontally placing the pseudo-bulb on the flat-bottom tray after removing the necrotic part. The tray was placed in a greenhouse with 50% sun-shading rate and sprayed 1 time per day to keep the water moss moist with pseudobulbs. Sprouting new buds and sprouting new roots from internodes of the pseudobulb within 4 months, and cutting the new buds from the pseudobulb when the height of the new buds is about 5cm, the sprouting number of the roots of the new buds is more than 5-6 and the length of the new buds is 2-3 cm; the cut buds are placed in a shade place for 2-3 days, wrapped by water moss after the wound is healed and temporarily planted in a nutrition cup.
4. And (3) planting the plant grown by the new bud after normal water and fertilizer management for 5 months, and realizing high-efficiency plant division propagation of the splash-ink type dendrobium nobile.
Example three: the 'red-white interphase' splash-ink dendrobium (flower color is red, petals and calyx edge have white radial stripes) is used as a female parent for plant division propagation.
1. Inverted cultivation of pseudobulb
In the eight bases of research institute of tropical crop variety resources of tropical academy of agricultural sciences in china in 7 months in 2020, a red-white interphase type dendrobium candidum plant which is growing inflorescence is selected, pedicles are cut off, the whole plant is taken out from a nutrition cup, a culture medium wrapping roots is removed, most of the roots are cut off, and then one cautious root of a clustered pseudobulb is separated (the root neck of the pseudobulb is not damaged when the root neck is separated, and a tool such as scissors can be used for assistance when necessary). Selecting a single false bulb with better activity, removing all leaves on the stem, turning the false bulb upside down, enabling the base part of the false bulb to face upwards and penetrate into the round hole at the bottom of the nutrition cup and expose the surface of the nutrition cup, and enabling the top end of the stem to face downwards and expose the outside of the round hole of the nutrition cup; wrapping the root neck part with culture medium such as coconut block, wherein the root neck part is about 1cm lower than the surface of the coconut block, moving the nutrition cup upwards to wrap the coconut block, placing the nutrition cup in an orchid tray, and placing the tray on a greenhouse orchid bedstead.
2. Division of plants in stage one
The pseudobulb cultivated in inverted state is subjected to normal cultivation management except fertilization, and is sprayed for 1 time every day according to weather conditions to keep the cultivation substrate moist. After the apical dominance is broken, the bud point at the base of the pseudobulb begins to sprout, the volume becomes larger, and the color becomes white after about 1 month. In the 2 nd month period, the bud grows up, turns green, and starts to grow upwards (at this time, the moisture and sufficient sunlight of the culture medium are ensured, so that the new bud grows robustly). By month 3, shoots began to sprout new roots. And when the sprout is about 5cm in height and the sprout number of the new root reaches more than 5-6 and the sprout length reaches 2-3 cm by the 4 th month, the new sprout can be cut from the stock plant. The cut buds are placed in a shade place for 2-3 days, and are wrapped by water moss after the wound is healed, and are temporarily planted in a 1.5-inch nutrition cup. After the first division, the pseudobulb as the mother plant was further cultivated in an inverted state for 3 months, and the plant was divided 1 time again in accordance with the growth of the sprout (the height of the sprout was about 5cm, the number of the sprouts in the roots reached 5 to 6, and the length reached 2 to 3 cm).
3. Second stage of plant division
Besides the root neck, internodes on the pseudobulb also have the ability of sprouting. Laying a layer of water moss for keeping moisture on the bottom of the flat-bottom tray, taking out the pseudo-bulb cultivated in an inverted manner from the nutrition cup, or cutting off the pseudo-bulb from the bottom of the nutrition cup, and horizontally placing the pseudo-bulb on the flat-bottom tray after removing the necrotic part. The tray was placed in a greenhouse with 50% sun-shading rate and sprayed 1 time per day to keep the water moss moist with pseudobulbs. Sprouting new buds and sprouting new roots from internodes of the pseudobulb within 4 months, and cutting the new buds from the pseudobulb when the height of the new buds is about 5cm, the root sprouting number of the new buds reaches more than 5-6 and the length reaches 2-3 cm; the cut buds are placed in a shade place for 2-3 days, and are wrapped by water moss after the wound is healed, and are temporarily planted in a nutrition cup.
4. And (3) planting the plant grown by the new bud after normal water and fertilizer management for 5 months, and realizing high-efficiency plant division propagation of the splash-ink type dendrobium nobile.
And (3) comparison test:
the conventional cultivation and division propagation mode is taken as a control example. Wherein, a mature plant of 'red-white interphase type' splash-ink dendrobium which is normally cultivated for two years is selected as a first control example (containing at least 2 pseudobulbs). Selecting a mature plant of 'red-green interphase type' splash-ink dendrobium which is normally cultivated for three years as a second control example (containing at least 3 pseudobulbs). The cultivation method comprises the following steps: planting in a nutrition cup, and carrying out normal cultivation management, wherein the cultivation time is one year. The plant division mode comprises the following steps: after the sprouts sprout, the plant is removed from the nutrition cup, the coconut shells at the root part are removed, the clustered pseudobulbs are carefully divided into two parts (the sprouts are required to be attached to at least 1 pseudobulb), the root part is wrapped by coconut pieces, the coconut pieces are planted in the nutrition cup and placed on an orchid tray for normal cultivation and management.
2. Identification of plant division propagation effect
1. Identification of budding effect of inverted cultivation of pseudobulb
In order to examine the sprouting of the pseudobulb of the splash-ink dendrobium, the sprouting time and the sprouting number in the above examples and comparative examples were observed and counted, and the sprouting number of each stock plant (plant) was calculated. The results are shown in Table 1.
TABLE 1 inverted cultivation of pseudobulb for sprouting
Remarking: a: the number of buds emerged for the first time; b: the number of buds emerged for the second time; c: the total number of sprouts when standing upside down; d: days are spaced before and after the first budding in the population; E. days are spaced before and after the second budding in the population.
The pseudobulb inverted cultivation budding result shows that the pseudobulb inverted cultivation mode is adopted, the top end advantages are eliminated, pseudobulb sprouting and new bud are effectively promoted, the budding number, the budding interval time (the interval time between the first bud sprouting and the last bud sprouting) and the budding time are remarkably different from those of a conventional cultivation division propagation mode, the division time is shortened, the propagation efficiency is improved, the production cost is reduced, the budding interval time is short, a foundation is laid for the follow-up improvement of the orderly growth of seedlings, and the batch production of the seedlings is facilitated.
2. Identification of pseudo bulb horizontal-laying moisturizing and sprouting effect
In order to examine the sprouting of the pseudobulb-keeping moisture-retaining means, the sprouting time and the number of sprouts in the above examples were observed and counted, and the number of sprouts per stock plant (pseudobulb) was calculated. The results are shown in Table 2. The total number of sprouts was counted and the proliferation factor was calculated and the results are shown in Table 3.
TABLE 2 sprouting of pseudobulbs on flat
TABLE 3 value-added Effect of different plant division modes
| Item | Number of stock plant (plant) | Total budding number (strain) | Multiplication factor (times) |
| Example one | 20 | 101 | 5.0 |
| Example two | 20 | 90 | 4.5 |
| EXAMPLE III | 20 | 107 | 5.4 |
| Comparison example 1 | 20 | 26 | 1.3 |
| Comparative example two | 20 | 23 | 1.2 |
The pseudo-bulb horizontal bud-out result shows that the measures of horizontal placement and moisture preservation of the pseudo-bulbs are adopted, the sprout-out capability among the pseudo-bulb joints can be effectively improved, the bud-out number is remarkably improved, the bud-out time is shortened, the bud-out interval time is short, the overall breeding efficiency is improved by 3-5 times, the method is remarkably higher than a conventional cultivation division propagation mode, the foundation is laid for the subsequent improvement of the neat growth of seedlings, and the batch production of the seedlings is facilitated.
3. Identification of division effect of splash-ink dendrobium
In order to examine the division condition of the splash-ink dendrobium, the division temporary planting number and the permanent planting number in the above examples and comparative examples are observed and counted, and the proliferation number and the permanent planting survival rate are calculated. The results are shown in Table 4.
TABLE 4 Splending situation of Dendrobium loddigesii Rolfe
| Item | Number of false plants | Determining the number of plants | Number of surviving plants | Survival rate |
| Example one | 60 | 60 | 60 | 100％ |
| Example two | 60 | 60 | 60 | 100％ |
| EXAMPLE III | 60 | 60 | 60 | 100％ |
| Comparison example 1 | 20 | 20 | 20 | 100％ |
| Comparative example two | 20 | 20 | 20 | 100％ |
The pseudo-bulb horizontal bud-forming result shows that the top end advantages are broken by adopting the measures of inverted cultivation and horizontal arrangement of the pseudo-bulbs, the germination and the new bud realization of the pseudo-bulbs are promoted, the plant division is realized rapidly, the planting survival rate is equivalent to that of the traditional plant division method, but the time is shortened by more than half, and the problems that the new buds of the dendrobium devonianum subjected to ink spraying grow slowly, the germination quantity is small, the plant division period is long, and the seedlings are difficult to produce in batches in the natural growth process are solved. Can be used for batch plant division propagation, realizes the production of a large number of plants and provides assistance for the industrialized development of the plants.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, it is possible to make various improvements and modifications without departing from the technical principle of the present invention, and these improvements and modifications should also be considered as the protection scope of the present invention.
Claims (3)
1. An efficient plant division propagation method of splash-ink type dendrobium nobile is characterized by comprising the following specific steps:
1) Cultivation of pseudobulb in handstand
Selecting an aged plant, taking out the whole plant, removing a culture medium wrapping the root, separating clustered pseudobulbs on the premise of not damaging the neck of the root, and performing inverted cultivation by taking the pseudobulbs as a parent plant;
2) First stage of division of plants
Carrying out normal cultivation management except fertilization on the pseudobulb cultivated in an inverted manner, keeping a cultivation medium moist, and cutting off new buds from a stock plant when new buds grow and sprout new roots at root neck bud points of the base part of the pseudobulb, wherein the height of the new buds is 4-6 cm, the root sprouting number of the new buds is more than 5-6, and the length of the new buds is 2-3 cm; placing the cut buds in a shade place for 2-3 days, wrapping the buds with water moss after the wound is healed, and temporarily planting the buds in a nutrition cup; continuously carrying out inverted cultivation on pseudo bulbs serving as mother plants for 2-3 months, carrying out plant division for 1 time again when the height of the buds is 4-6 cm, the number of the germinated roots is more than 5-6 and the length is 2-3 cm;
3) Second stage of division of plants
Laying a layer of water moss for keeping moisture on the bottom of the tray, taking out the pseudo-bulbs cultivated upside down, removing necrotic parts, and flatly placing the pseudo-bulbs on a flat-bottom tray; placing the tray in a greenhouse with a sun shading rate of 40-60%, spraying for 1 time every day to keep the pseudobulbs moist, and cutting off the buds from the pseudobulbs when the internodes of the pseudobulbs germinate buds and sprout new roots, the height of the new buds is 4-6 cm, the sprouting number of the new buds reaches more than 5-6, and the length reaches 2-3 cm; placing the cut buds in a shade place for 2-3 days, wrapping the buds with water moss after the wound is healed, and temporarily planting the buds in a nutrition cup;
4) And planting the plant grown by the new bud after normal water and fertilizer management for 3-6 months to realize high-efficiency separate propagation of the splash-ink type dendrobium.
2. The efficient plant division propagation method of the splash-ink type dendrobium nobile according to claim 1, characterized in that: the mature plants are plants that have bloomed or are inflorescence-producing.
3. The efficient plant division propagation method of the splash-ink type dendrobium nobile according to claim 1, characterized in that: the inverted cultivation method comprises the following steps: selecting a strong single pseudobulb, removing all leaves on the stem, penetrating the base part of the pseudobulb upwards from a round hole at the bottom of a nutrition cup and exposing the surface of the nutrition cup, and exposing the top end of the stem downwards from the round hole of the nutrition cup, then wrapping the root neck part with a culture medium, wherein the position of the root neck part is 0.5-1.5 cm lower than the surface of the culture medium, wrapping the culture medium with the nutrition cup and placing the culture medium on an orchid culture bed frame.
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