CN114128580B - Lily bulb breeding method - Google Patents

Lily bulb breeding method Download PDF

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Publication number
CN114128580B
CN114128580B CN202111418882.XA CN202111418882A CN114128580B CN 114128580 B CN114128580 B CN 114128580B CN 202111418882 A CN202111418882 A CN 202111418882A CN 114128580 B CN114128580 B CN 114128580B
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lily
plant
breeding
planting
soil
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CN114128580A (en
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杨凤萍
张秀海
薛静
张铭芳
陈绪清
杜运鹏
高俊莲
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Beijing Academy of Agriculture and Forestry Sciences
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Beijing Academy of Agriculture and Forestry Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/35Bulbs; Alliums, e.g. onions or leeks
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/60Flowers; Ornamental plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

Abstract

The invention discloses a lily bulb breeding method, which comprises the following steps: planting lily bulbs; (2) bending treatment: inserting bamboo poles at the periphery of the plants, pulling the tops of the plants to bend towards the bamboo poles by hands, and fixing branches on the bamboo poles by using a binding belt to enable the plants to grow in a bent mode; (3) field water and fertilizer management; and (4) harvesting the pearl buds. The breeding method of the lily bulbs provides a new way for obtaining seed sources for lily production, is simple and feasible, saves the production cost, saves the time and labor for bulb cultivation, and can obtain high-quality breeding materials.

Description

Lily bulb breeding method
Technical Field
The invention relates to the field of garden plant cultivation, in particular to a lily bulb breeding method.
Background
Lily (Lilium spp.) is a perennial herb root flower of the Lilium genus (Lilium) of the Liliaceae family (Liliaceae) of monocotyledonous plants, and is one of the important species in the bulbous flower industry. Lily is widely used as cut flower, pot plant and garden plant in the world. The lily has higher nutritive value, health-care function and ornamental property, and is one of important medicinal, edible and ornamental plants, so that the industrial demand is increased day by day and the market prospect is wide. At present, the lily industry in China is only in a starting stage, and a low-cost, high-yield and high-quality seed ball production technology is urgently needed to promote the rapid development of the lily industry in China. The growth period of lily in a seed ball development mode through a seed embryo is long (generally more than two to three years), the demands of lily flowers and seed balls on the market cannot be met, and the character of the plant progeny bred through the seed embryo can be separated, so that excellent characters cannot be stably maintained. Therefore, the asexual propagation mode is the best way to obtain the lily bulbs.
In the main production area of edible lily in China, small seed balls separated when the seed balls are harvested in the same year are mainly used as seed sources for planting lily in the next season. In the growing process of lily, when the overground part of the plant reaches the late development stage, the periphery of the mother bulb can rapidly expand and develop into new bulblets, and each bulblet can continue to develop into a new bulb individual. However, this method of forming new bulbs by differentiation from mother bulbs is only suitable for lily with large bulb volume, and has low propagation efficiency, and cannot form a perfect propagation system.
The scale is one of the constitutive organs of the bulb, formed by the abnormal leaves that colonize around the bulb pan, and the bulb pan constitutes the bulb. At present, lily bulbs used for cut flower production are mainly cultivated by means of scale cuttage. However, a large amount of seed balls can be consumed by culturing the small seed balls through the scales, the quality requirement on the seed balls is higher, and the high-quality seed balls are easily cultured by the scales without diseases and insect pests and with the thickness.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a lily bulb breeding method which is low in cost and simple and convenient to operate.
The invention discloses a breeding method of lily bulbs, which comprises the following steps:
(1) Planting lily bulbs;
(2) Bending treatment, wherein the treatment time is one of the following two types:
I. lily without looking for flowers: directly carrying out topping and flower removal treatment and then carrying out bending treatment when the plants enter the flowering period;
lily requiring flower observation: when lily plants enter the late growth stage, namely, petals begin to wither and underground seed balls begin to expand,
performing bending treatment;
the bending treatment mode is as follows: inserting bamboo poles at the periphery of the plants, pulling the tops of the plants to bend towards the bamboo poles by hands, and fixing branches on the bamboo poles by using a binding belt to enable the plants to grow in a bent mode;
(3) Managing field water and fertilizer;
(4) And (5) harvesting the bulbels.
The breeding method of the lily bulbs, disclosed by the invention, is characterized in that the bending growth angle is 90 degrees.
The breeding method of the lily bulb comprises the steps of bending 1/3 of the overground stem of a plant at the upper part, and keeping other stem parts in an upright state.
The breeding method of the lily bulbs, disclosed by the invention, is characterized in that the height of the bamboo poles is 80cm.
The breeding method of the lily bulbs, disclosed by the invention, is characterized in that the lily is an Asian series lily variety Lilium 'Golden Stone'.
The breeding method of the lily bulbs, disclosed by the invention, comprises the following steps of (1):
preparing soil treatment: deeply digging soil by 30cm, paving an insect-proof non-woven fabric, mixing matrix soil and the soil according to a ratio of 1;
planting seed balls: the planting depth of the seed balls is 3-5cm, and planting is carried out according to the planting density of 30cm of plant spacing and 50cm of row spacing.
The lily bulb breeding method is characterized in that in the step (3), watering is carried out in the early growth stage of lily according to the dry and wet condition of soil, when the plant grows to enter the flowering phase and the underground bulb begins to expand, a high-nitrogen and low-phosphorus high-potassium compound fertilizer needs to be additionally applied for 2 times, and the supplementary application is carried out at the interval of 2 months.
According to the breeding method of the lily bulbs, provided by the invention, in the step (4), when plant stalks are basically withered and yellow and bulbels grow into an independent individual, the bulbels are directly picked.
The breeding method of the lily bulbs of the invention is different from the prior art in that:
the bulbil is an overground organ that grows in axillary buds, originates from axillary meristems, and is similar to tillering in arabidopsis thaliana and rice. The formation of the bulbil can be divided into two processes: (1) The leaf axillary meristematic cells are continuously divided to form bulbil primordium; and (2) the bulbil primordium grows and differentiates to form a bulbil structure. The bulbil and bulb have the same nature, and are the reproductive organs of lily, but the implantation parts are different. Bulbs grow in the underground part and bulbels grow in the overground part. The mature bulbils fall to the ground and begin to develop into bulbs. As the bulbels grow at the axillary parts of the leaves of the plants, do not contact with the soil, have no influence of soil-borne diseases, basically do not carry the lily viruses, and seedlings cultured by using the lily bulbels have no viruses. As a propagation material, the quality is good and the harvest is easy. However, not all lilium plants can form bulbels, and at present, four wild species of lilium brownii, namely lilium tigrinum, lilium luteum, lilium sanguinatum and lilium bulbiferum, are known to form bulbels naturally at axillary buds in stems, but the propagation method is only applicable to partial lilium materials with bulbels.
Through years of cultivation observation, the Asian series lily is found to grow 1-2 bulbels at the top of the plant in the later growth period of the plant. Therefore, the invention develops a key technology by using Asian series lily varieties (Lilium 'Golden Stone'), and can obtain a plurality of high-quality bulbels at the later growth stage of plants for lily propagation by a special cultivation mode. The method provides a new approach for obtaining seed sources for lily production, is simple and feasible, saves the production cost, saves the time and labor for seed bulb cultivation, and can obtain high-quality propagation materials.
The breeding method of lily seedball of the invention is further explained with the attached drawings.
Drawings
FIG. 1 is a photograph of a lily plant bent according to the present invention;
FIG. 2 is a photograph of another angle of a bent lily plant of the present invention;
FIG. 3 is a photograph of an entirety of a plurality of bent lily plants of the present invention;
FIG. 4 is a photograph of a bulbil harvested from a lily plant using the method of the present invention.
Detailed Description
1. Experimental Material
Asian series lily variety Lilium 'Golden Stone', the plant height is 30-45cm, the seed ball circumference is about 76-150mm, and the lily is fond of yang and shade-tolerant.
2. Planting of Experimental materials
Preparing soil treatment: the soil is dug deeply by 30cm, the insect-proof non-woven fabric is laid, the matrix soil (filled with organic matters more than or equal to 45 percent, humic acid more than or equal to 25 percent, nitrogen phosphorus potassium more than or equal to 3 percent, medium trace elements more than or equal to 1 percent and pH5.5-6.5) and the soil are mixed according to the proportion of 1.
Planting seed balls: the planting depth of the seed ball is 3-5cm, and planting is carried out according to the planting density of 30cm of plant spacing and 50cm of row spacing.
3. Key technology management (fig. 1-3)
3.1 when the plant enters the flowering period, if no flower observation is needed, directly carrying out topping and flower removal treatment. Inserting a bamboo pole 80cm high around the plant (e.g. in front), bending the top of the plant by more than 90 degrees to the side (side of the bamboo pole) by hand (this angle is most effective), and then fixing the branch on the bamboo pole by a bandage to make the plant bend by 90 degrees for growth. In order to prevent the branches from being broken, one third of the stems on the ground are kept at the upright part, and the normal growth of the plants is ensured.
3.2 when the lily needs to be observed, when the lily plant enters the late growth stage (petals begin to wither) and the underground seedball begins to expand, a bamboo pole with the height of 80cm is inserted into the periphery (for example, the front) of the plant, the top of the plant is pulled by hands to bend 90 degrees towards the side (the side of the bamboo pole), and then the branch is fixed on the bamboo pole by a bandage to enable the plant to bend 90 degrees for growth. In order to prevent the branches from being broken, one third of the branches on the ground are reserved, and the stalks are positioned on the vertical parts to ensure the normal growth of the plants.
4. Management of fertilizer and water in field
In the early growth stage of lily, the lily is planted by using the matrix mixed soil, so that the fertility is sufficient, additional fertilization is not needed, and watering is needed according to the dry and wet conditions of the soil. When the plant grows into the flowering phase, the underground bulb begins to expand and carries out lateral growth adjustment, at the moment, the axillary part of the stem leaf begins to breed the bulbil, the high-nitrogen, low-phosphorus and high-potassium compound fertilizer needs to be additionally applied for 2 times, and the supplementary application is carried out at the interval of 2 months.
5. Harvesting of pearl buds
After the plant blooms, the plant can continue to grow for 4-5 months in the later growth period so as to meet the expansion of the underground bulb and the growth of the bulbil. When the plant stem is basically withered and yellow, the bulbil grows into an independent individual, and the bulbil can be directly picked.
6. Statistics of results
The bulbels obtained by the method can obtain 1-2 bulbels at each axillary of the leaf after the bending and drawing of the plant, and the whole plant can obtain 10-20 bulbels (figure 4 is a bulbel picture harvested from one plant) which are far more than the number of bulbels generated by the underground bulb. The top bulbil is the largest, the diameter can reach 3.5cm, and the weight of a single seed can reach 6.87g. More than 50% of the bulblets can have the diameter of 1.5-2cm, which reaches the standard of the commercial lily bulbs.
The plant bending treatment planting method can increase the sprouting quantity of the bulbil at the axillary position of the lily plant leaf by more than 10 times. The bulbil is used as a seed ball for production, has good quality and high quality, does not have soil and pest, and can save a large amount of seed balls for scale cutting propagation (a main propagation mode used in production). 4,400 lilies can be planted per mu according to the planting density of 30cm plant spacing and 50cm row spacing, and 44,000-88,000 bulbil can be obtained by the treatment in the way, thus the economic benefit is very objective.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.

Claims (7)

1. A lily bulb breeding method is characterized in that: the method comprises the following steps:
(1) Planting lily bulbs;
(2) Bending treatment, wherein the treatment time is one of the following two types:
I. lily without looking for flowers: directly carrying out topping and flower removal treatment and then carrying out bending treatment when the plants enter the flowering period;
lily requiring flower observation: when lily plants enter the late growth stage, namely petals begin to wither and underground seed balls begin to expand, bending treatment is carried out;
the bending treatment mode is as follows: inserting bamboo poles at the periphery of the plant, pulling the top of the plant to bend towards the direction of the bamboo poles by hands, and fixing branches on the bamboo poles by using a binding tape to enable the plant to grow in a bent manner;
(3) Managing field water and fertilizer;
(4) Harvesting the bulbels;
the lily is a Asian series lily variety Lilium 'Golden Stone'.
2. The method for breeding lily bulbs according to claim 1, wherein the method comprises the following steps: the angle of the curved growth is 90 °.
3. The method for breeding lily bulbs according to claim 2, wherein: bending 1/3 length of the above-ground stalk of the plant, and keeping other stalk parts in an upright state.
4. The method for breeding lily bulbs according to claim 3, wherein: the height of the bamboo pole is 80cm.
5. The method for breeding lily bulbs according to claim 4, wherein the method comprises the following steps: the step (1) comprises the following steps:
preparing soil treatment: deeply digging soil by 30cm, paving an insect-proof non-woven fabric, mixing matrix soil and the soil according to the proportion of 1;
planting seed balls: the planting depth of the seed balls is 3-5cm, and planting is carried out according to the planting density of 30cm of plant spacing and 50cm of row spacing.
6. The method for breeding lily bulbs according to claim 5, wherein: in the step (3), watering is carried out in the early growth stage of the lilies according to the dry and wet condition of soil, and when the underground bulbs begin to expand after the plants grow into a flowering phase, additionally applying a high-nitrogen low-phosphorus high-potassium compound fertilizer for 2 times, and carrying out supplementary application at an interval of 2 months in the middle.
7. The method for breeding lily bulbs according to claim 6, wherein: in the step (4), when plant stems are basically withered and yellow and the bulbels grow into an independent individual, the bulbels are directly picked.
CN202111418882.XA 2021-11-26 2021-11-26 Lily bulb breeding method Active CN114128580B (en)

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Citations (4)

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Publication number Priority date Publication date Assignee Title
US5138794A (en) * 1990-03-28 1992-08-18 The United States Of America As Represented By The Secretary Of Agriculture Method for producing lilium elegans
JP2000188944A (en) * 1998-12-28 2000-07-11 Shoji Kobayashi Production of garlic utilizing bulbil
CN110495394A (en) * 2019-08-22 2019-11-26 中国农业科学院蔬菜花卉研究所 A kind of rapidly and efficiently propagation method of lily tiger lily stem section in-vitro inducing tuberization
CN113151353A (en) * 2021-06-04 2021-07-23 中国农业科学院蔬菜花卉研究所 Lily LlKnat13 gene clone and application method thereof in controlling formation of lily bulbil

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Publication number Priority date Publication date Assignee Title
JP2008178388A (en) * 2006-12-28 2008-08-07 Sanken Shokuhin Kk Method for proliferation of allium sativum
CN102742442A (en) * 2012-07-03 2012-10-24 云南普瑞生物制药有限公司 Wild pinellia ternate cultivation method
CN104396742B (en) * 2014-10-30 2017-03-15 毕节市中药研究所 The method that aseptic seedling is differentiated again with five-step approach induction Lilium sulphureum Baker bulbil calluss

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5138794A (en) * 1990-03-28 1992-08-18 The United States Of America As Represented By The Secretary Of Agriculture Method for producing lilium elegans
JP2000188944A (en) * 1998-12-28 2000-07-11 Shoji Kobayashi Production of garlic utilizing bulbil
CN110495394A (en) * 2019-08-22 2019-11-26 中国农业科学院蔬菜花卉研究所 A kind of rapidly and efficiently propagation method of lily tiger lily stem section in-vitro inducing tuberization
CN113151353A (en) * 2021-06-04 2021-07-23 中国农业科学院蔬菜花卉研究所 Lily LlKnat13 gene clone and application method thereof in controlling formation of lily bulbil

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吴姝菊.药用观赏花卉百合的引种栽培技术.黑龙江农垦师专学报.2003,(02),第71-73页. *

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