CN112913488A - Comprehensive method for rapid vegetative propagation of peony - Google Patents
Comprehensive method for rapid vegetative propagation of peony Download PDFInfo
- Publication number
- CN112913488A CN112913488A CN202110183398.7A CN202110183398A CN112913488A CN 112913488 A CN112913488 A CN 112913488A CN 202110183398 A CN202110183398 A CN 202110183398A CN 112913488 A CN112913488 A CN 112913488A
- Authority
- CN
- China
- Prior art keywords
- soil
- branch
- planting
- branches
- peony
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/20—Layering
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of plant planting, in particular to a peony asexual propagation method. The invention discloses a comprehensive method for asexual propagation of peony. The invention comprises the steps of mother plant cultivation, mother plant oblique cultivation layering, young new branch, new branch horizontal constriction/ring cutting, root promotion, seedling separation, transplantation and the like. Is characterized in that (1) the breeding efficiency is high, and each branch of the mother plant can breed 3-4 seedlings; (2) the rooting is fast, and seedlings can be transplanted in 3 months; (3) the rooting rate is high, and the rooting rate of the first-level root and the second-level root of the seedling can reach 100 percent; (4) large root volume and developed root system. The average number of the first-grade roots is 8.20 per seedling, the average length of the first-grade roots is 10.22cm, and the average thickness is 1.96 mm; (4) the survival rate of the transplantation is high, namely the survival rate of the transplantation of the rooted seedlings reaches 100 percent. (5) The technology is simple and easy to operate, special management is not needed after treatment, materials are easy to obtain, the cost is low, and no harm is caused to operators.
Description
Technical Field
The invention relates to the technical field of plant planting, in particular to a peony asexual propagation method.
Background
Peony is a shrub of peony group of Paeonia of Paeoniaceae, and is a unique traditional famous flower in China. The cultivation history is long, the flower is big and colorful, and the reputations of "national beauty and fragrance". Deciduous shrubs, two or three compound leaves, blossom in spring. Besides appreciation, peony can be used as a medicine in roots and can extract oil in seeds, and is one of novel woody oil crops. The peony is suitable for wide planting range, and China has planting except Hainan province. The method is characterized in that famous large-scale special gardens in Luoyang, Shandong lotus, Qingdao, Shaanxi Xian and the like are arranged in various parks, public green lands, residential areas and street green belts. Anhui Bozhou, Tongling and Chongqing Zuijiang are the main producing areas of the Chinese medicinal material "moutan bark".
The seed seedling culture is suitable for culturing peony grafting stocks and medical and oil peony seedlings, the propagation coefficient is high, but the improved seeds and ornamental varieties are not suitable for seeding and propagation. The excellent peony seeds and ornamental peony varieties need asexual propagation, and the excellent characteristics of the offspring can be maintained. Grafting and plant division are the current main asexual propagation methods of the peony. The difficulty of the plant division propagation technology is low, and the propagation coefficient is also low. The peony grafting propagation technology has high requirements, 2 scions can be cut from each branch of a stock plant, the propagation coefficient is low, and the survival rate is influenced by various factors such as the quality of the stock, the grafting period, the healing of the grafted stock, the operation proficiency of grafting workers, the management after grafting and the like. Although the plant cuttage propagation coefficient is high, the peony cuttage difficulty is higher, the rooting rate is low, and the rooting time is long; the cutting time and the environment after cutting are regulated and controlled in the cutting process, particularly the air relative humidity and the cutting substrate humidity are high in control requirement, and the cutting rooting rate difference among varieties is large; although the technical difficulty of layering propagation is low, the rooting rate is relatively low and the rooting time is long. The peony tissue culture technology is still imperfect, and the technical difficulty and the cost are higher. The efficient asexual propagation technology is the basis of the popularization and industrialization of the excellent variety of the peony.
Disclosure of Invention
The invention aims to provide a comprehensive technology for peony asexual propagation, which has high rooting rate, developed root system, fast seedling formation and higher propagation coefficient, and combines layering branch promotion, branch rejuvenation, branch injury and hormone root promotion. The method has the advantages of simple operation, small technical difficulty and low cost, and can effectively overcome the difficulties of large technical operation and maintenance difficulty, high cost, slow rooting and long time consumption in the conventional peony asexual propagation. The technology has the advantages of strong operability, high efficiency, simple management and low cost, and can obviously improve the economic benefit of seedling culture.
Aiming at the dilemma faced by peony propagation in the background technology, the invention adopts the technical scheme that:
mother plant cultivation: 1 year ahead, after the autumn peony leaves, the overground parts (namely all branches) of the 1-3 year old breeding mother plants are subjected to stumping treatment, and winter fertilizer is applied. And (4) germinating a plurality of new branches at the root neck part of the parent plant in the spring of the next year, and performing conventional management in the growing season. In autumn of the same year, the weak branches are removed from the base, and only robust branches without diseases and insect pests are reserved. At the moment, the root system of the mother plant grows for 2-4 years, the branch age is only 1 year, and strong plants without diseases and insect pests are selected, and the seedlings are lifted by bare roots.
Oblique planting of a stock plant: soil preparation is carried out at least 1 month before planting. The soil is required to be loose and fertile, the organic matter is rich, the drainage is good, the pH value is 6.5-7.5, the soil preparation depth is at least 50cm, and the soil pest control is the same as the conventional management measures.
Preparing a planting hole: the row spacing of the planting holes is 50cm multiplied by 100cm, the depth is 30cm, the planting holes incline towards one side of the row spacing, the included angle between the inclined plane and the horizontal ground is 15-20 degrees, the included angle between the inclined plane and the horizontal ground is preferably 15 degrees, and the planting holes are arranged in a shape like a Chinese character 'pin'.
Layering the stock plant:
the root system of the bare-root stock plant is placed on the inclined plane of the planting hole to spread the root system, the branch of the stock plant is horizontally fixed on the ground by a U-shaped ground nail, the planting soil is backfilled in the hole layer by layer, and the planting soil is trampled. Then, loose fertile planting soil with rich organic matters and good drainage and pH of 6.5-7.5 is piled up to 30cm in height on the ground, all branches can be covered with the soil pile, and bamboo poles with the diameter of 1cm are inserted into the soil pile for 1-2 branches to facilitate the ventilation of the branches in the soil pile. When the soil temperature changes from "day to night freezing", namely above 0 ℃ in the day and when the temperature drops below 0 ℃ at night, the soil temperature is irrigated with enough "frozen water" to facilitate the branches to pass the winter.
Promoting the growth of new branches and rejuvenating bases:
before germination in the spring of the next year, commercial organic fertilizer is spread among each planting row (the fertilizing amount is different according to the selected fertilizer). After fertilization, watering is combined, and the soil is turned into. Along with the rise of the temperature, each branch of the mother plant under the hilling pile sprouts 3-4 new branches on average and gradually extends out of the soil surface, and if buds exist, the new branches are removed in time. The hilling pile is reserved to facilitate the base of the new branch to keep young. Weeds are removed at any time, and daily management is carried out according to the conventional peony growing season.
Treating new branch injury:
in 4-7 months of the year, when the area of the new branch leaves stops increasing, the most preferable is 6 months in the North China and 5 months in the south China; preferably 7 months in North China and 6 months in south China. Selecting strong new branches without diseases and insect pests, digging up the culture soil heap, and making a wound on the base of each branch upwards at a position of 3-5 cm. Most preferably, the wound is transversely contracted by a self-locking white nylon cable tie (with the length of 150mm and the width of 2.7mm), and the cable tie is tightly contacted with the branch without a gap; preferably a sharp blade girdling layer sterilized with 800 times carbendazim: the girdling length is 2/3 g of the circumference of the branch, and xylem is visible deeply. The girdling width is 1.5cm, preferably 1.5 cm.
And (3) promoting the root of the new branch:
and (3) placing a cotton ball soaked with a root-promoting hormone solution on the branch injury site for 10 minutes and then removing the cotton ball. Most preferably the hormone and concentration is IBA (indolebutyric acid) 1200mg/L, more preferably IBA500, preferably NAA (naphthylacetic acid) 500 mg/L. And then restoring the height of the original culture soil pile, covering all the treated branches again, and compacting the branches properly to ensure that each branch is in close contact with the soil, and keeping the relative water content of the culture soil pile to be 60-80% so as to facilitate the rooting of the branches. Normal field management, and the specific measures are the same as the conventional management of the peony growing season.
Separating rooted shoots/(seedlings):
in the autumn leaf fall period, the ridging pile is opened, and a large number of roots grow out of each branch at the binding position or the girdling position of the binding belt, and the roots are thick and strong and have first-level and second-level roots. And (4) cutting the rooted branches to obtain new seedlings. Placing in shade place for planting.
Seedling planting:
the land preparation requirement is the same as that of the mother plant. The specification and row spacing of the planting holes are suitable for the root systems of the seedlings, planting soil is firstly backfilled in the planting holes, the seedlings are placed in the centers of the holes and are backfilled layer by layer, and the seedlings are compacted while being backfilled. Finally, the neck of the seedling root is piled with soil piles to facilitate winter passing. The same stock plant is required for freezing water. The next year growth period management is the same as the conventional peony seedling management.
Compared with the prior art, the technology has the advantages that (1) the stock plant cultivated by the method has the characteristics that the root system is 3-4 years old, and the age of the new branch is 1 year after stumping. The mother plant has developed root system, rich nutrient storage and strong water and fertilizer absorption capacity; the small branch age has strong growth potential, which is beneficial to the sprouting of more new branches after layering, and each branch layering of the stock plant cultivated by the invention can sprout 3-4 new branches. (2) The invention rejuvenates the new branch generated after the stock plant is layered, thereby slowing down the lignification speed of the new branch, keeping the tender of the branch tissue and being beneficial to the formation and development of root primordia. (3) The method for making wounds on 2 branches with transverse constriction and circular stripping of the new branch is most preferably a self-locking white binding belt, is low in cost, more convenient than screwing iron wires by using hand pliers, simple and fast to operate, high in efficiency and less in damage to the branches, and the transverse constriction has the functions of accumulating nutrition and promoting rooting. The plastic binding belt is used for replacing a common iron wire, so that the defects that the bark of a rejuvenated branch is damaged and the tail of the tightened iron wire hurts hands frequently caused by seedling lifting are avoided. (4) According to the invention, after 3 months of treatment by the plant root-promoting hormone, the rooting rate of the first-level root and the second-level root of the branch can reach 100%; the root system of the seedling is developed and strong, the average number of first-grade roots is 8.20 per branch, the average number of first-grade roots is 10.22cm long, and the average thickness is 1.96 mm; (5) after the branches are treated, the branches can be managed conventionally under natural conditions, and special environmental regulation measures are not needed. (6) The invention integrates layering, rejuvenation, transverse/annular peeling and root promoting treatment, and has high seedling rooting rate, fast rooting, developed root system and large root quantity. The survival rate of bare root transplantation of the seedlings is 100 percent, the production cost is reduced, and the economic benefit is increased.
Description of the drawings:
FIG. 1: section and plan of mother plant planting hole
FIG. 2 rooting status of new shoots after 3 months of wounding and root promoting treatment
Detailed Description
The scheme of the invention is further illustrated by the following examples:
example 1:
mother plant cultivation:
(1) in 2018, after the leaves of the peony in autumn are fallen, all branches on the ground of the 2-year peony variety 'xiangyu' are subjected to stumping treatment, and then winter fertilizer is applied.
(2) And (4) germinating a plurality of new branches at the root neck part of the parent plant in 2019 spring, and performing conventional management in growing seasons. And 9 months in 2019, removing the weak branches from the base, and only keeping robust annual branches without diseases and insect pests, wherein the root system of the mother plant is 3 years, and the branch age is only 1 year.
(3) Selecting strong stock plants without diseases and insect pests, and taking seedlings from bare roots.
Oblique planting of a stock plant:
(1) land preparation: soil preparation is completed in advance at the end of 7 months in 2019. The soil of the land parcel is loose and fertile, the organic matter is rich, the drainage is good, the pH is 7.0, the deep ploughing is 50cm, and the prevention and control of the soil diseases and insect pests are managed with the conventional method.
(2) Preparing a planting hole: in 2019, in 9 months, the row spacing is 50cm multiplied by 100cm, the depth is 30cm, the planting holes are inclined towards one side of the row spacing, the included angle between the inclined plane and the horizontal ground is 15 degrees, and the planting holes are arranged in a shape like a Chinese character 'pin'.
Layering the stock plant:
(1) the root system of the bare-root stock plant is placed on the inclined plane of the planting hole to spread the root system, the branch of the stock plant is horizontally fixed on the ground by a U-shaped ground nail, the planting soil is backfilled in the hole layer by layer, and the planting soil is trampled.
(2) The cultivation method is characterized in that loose fertile planting soil is used, organic matters are rich, water drainage is good, planting soil with the pH value of 7.0 is piled up to be 30cm high on the ground, all branches of a mother plant can be covered by the cultivation soil, and 2 bamboo poles with the diameter of 1cm are inserted into the soil pile to facilitate ventilation of the branches in the soil pile.
(3) When the soil temperature changes from "day to night freezing", namely above 0 ℃ in the day and when the temperature drops below 0 ℃ at night, the soil temperature is irrigated with enough "frozen water" to facilitate the branches to pass the winter. Promoting new branches and keeping the base young:
(1) before germination in spring of 2020, commercial organic fertilizer is scattered among each planting row, and the application amount meets the requirements of the specification. After fertilization, the fertilizer is combined with spring irrigation and is turned into soil.
(2) Then, each branch of the mother plant under the soil pile sprouts 3.5 new branches on average and gradually extends out of the soil surface, and buds on the new branches are removed in time. The hilling pile needs to be reserved so as to be beneficial to the young base of the new branch. Weed is removed at any time, and pest control is carried out regularly.
Injury is caused at the base of the new branch:
(1) in the middle and last ten days of 6 months in 2020, when the area of the leaves of the new branches stops increasing, selecting robust new branches without diseases and insect pests, and digging up the soil heap;
(2) transverse constriction: binding the position 4cm above the base of each branch tightly with a white self-locking nylon binding belt (with the length of 150mm and the width of 2.7 mm); and (3) promoting the root of the new branch:
(1) a cotton ball soaked with IBA1200mg/L of the solution was placed in the tight area of the band and removed after 10 minutes.
(2) And then restoring the original culture soil pile, covering all the treated branches again, and compacting the branches properly to ensure that each treated branch is in close contact with the soil and the relative water content of the culture soil pile is kept between 60 and 80 percent.
(3) Normal field management, and regular prevention and control of plant diseases and insect pests, and the specific measures are the same as the conventional management measures for the peony growing season.
Separating rooted branches/seedlings:
(1) in late 9 months, the soil covering piles are pulled open, a large number of roots grow out of each branch at the binding position of the binding belts or near the girdling position, the rooting rate is 100%, the roots are thick and strong, and secondary roots exist. The average number of the first-level roots is 7.9 per branch, the average root length is 12.6cm, and the average root thickness is 2.2 mm.
(2) Cutting the rooted branch with sharp tool to obtain new seedling. Placing in shade place for planting.
Seedling planting:
(1) the land preparation requirement is the same as that of mother plant planting; the planting holes are 20cm multiplied by 20cm, and the planting row spacing is 20cm multiplied by 30.
(2) And backfilling 5cm of planting soil in the planting holes, placing the seedlings in the center of the planting holes, and backfilling layer by layer while treading. The neck of the seedling root is piled with 10cm high soil to facilitate the winter.
(3) The frozen water is required to be cultivated with the same mother plant, and the management in the next year growth period is the same as the conventional peony seedling management.
---------------------------------------------------------------------
Example 2:
mother plant cultivation:
(1) in 2018, after the leaves of the peony in autumn are fallen, all branches of the 3-year peony variety 'riches and honour full of hall' on the ground are subjected to stumping treatment, and then winter fertilizer is applied.
(2) And (4) germinating a plurality of new branches at the root neck part of the parent plant in 2019 spring, and performing conventional management in growing seasons. And 9 months in 2019, removing the weak branches from the base, and only keeping robust annual branches without diseases and insect pests, wherein the root system of the mother plant is 4 years and the branch age is only 1 year.
(3) Selecting strong stock plants without diseases and insect pests, and taking seedlings from bare roots.
Oblique planting of a stock plant:
(1) land preparation: soil preparation is completed in advance at the end of 6 months in 2019. The soil of the land parcel is loose and fertile, the organic matter is rich, the drainage is good, the pH is 7.0, the deep ploughing is 50cm, and the prevention and control of the soil diseases and insect pests are managed with the conventional method.
(2) Preparing a planting hole: in 2019, in 9 months, the row spacing is 50cm multiplied by 100cm, the depth is 30cm, the planting holes are inclined towards one side of the row spacing, the included angle between the inclined plane and the horizontal ground is 20 degrees, and the planting holes are arranged in a shape like a Chinese character 'pin'.
Layering the stock plant:
(1) the root system of the bare-root stock plant is placed on the inclined plane of the planting hole to spread the root system, the branch of the stock plant is horizontally fixed on the ground by a U-shaped ground nail, the planting soil is backfilled in the hole layer by layer, and the planting soil is trampled.
(2) The cultivation method is characterized in that loose fertile planting soil is used, organic matters are rich, water drainage is good, planting soil with the pH value of 7.0 is piled up to be 30cm high on the ground, all branches of a mother plant can be covered by the cultivation soil, and 2 bamboo poles with the diameter of 1cm are inserted into the soil pile to facilitate ventilation of the branches in the soil pile.
(3) When the soil temperature changes from "day to night freezing", namely above 0 ℃ in the day and when the temperature drops below 0 ℃ at night, the soil temperature is irrigated with enough "frozen water" to facilitate the branches to pass the winter. Promoting new branches and keeping the base young:
(1) before germination in spring of 2020, commercial organic fertilizer is scattered among each planting row, and the application amount meets the requirements of the specification. After fertilization, the fertilizer is combined with spring irrigation and is turned into soil.
(2) Then, each branch of the mother plant under the soil pile sprouts 3 new branches on average and gradually extends out of the soil surface, and buds on the new branches are removed in time. The hilling pile needs to be reserved so as to be beneficial to the young base of the new branch. Weed is removed at any time, and pest control is carried out regularly.
Injury is caused at the base of the new branch:
(1) in the middle 6 th month of 2020, when the area of the leaves of the new branches stops increasing, selecting robust new branches without diseases and insect pests, and digging up the soil heap;
(2) transverse constriction: binding the position 4cm above the base of each branch tightly with a white self-locking nylon binding belt (with the length of 150mm and the width of 2.7 mm);
and (3) promoting the root of the new branch:
(1) a cotton ball soaked with IBA500mg/L of the solution was placed in the tight area of the band and removed after 10 minutes.
(2) And then restoring the original culture soil pile, covering all the treated branches again, and compacting properly to ensure that each treated branch is in close contact with the soil and the relative water content of the culture soil pile is kept between 60 and 80 percent.
(3) Normal field management, and regular pest control, and the specific measures are the same as the conventional management measures of the peony growing season.
Separating rooted branches/seedlings:
(1) in the middle ten 9 months, the soil covering piles are pulled open, a large number of roots grow out of each branch at the binding position of the binding belts or near the girdling position, the rooting rate is 100%, the roots are thick and strong, and secondary roots exist. The average number of the first-level roots is 7.2 per branch, the average root length is 8.7cm, and the average root thickness is 1.6 mm.
(2) Cutting the rooted branch with sharp tool to obtain new seedling. Placing in shade place for planting.
Seedling planting:
(1) the land preparation requirement is the same as that of mother plant planting; the planting holes are 20cm multiplied by 20cm, and the planting row spacing is 20cm multiplied by 20 cm.
(2) And backfilling 5cm of planting soil in the planting holes, placing the seedlings in the center of the planting holes, and backfilling layer by layer while treading. The neck of the seedling root is piled with 10cm high soil to facilitate the winter.
(3) The frozen water is required to be cultivated with the same mother plant, and the management in the next year growth period is the same as the conventional peony seedling management.
---------------------------------------------------------------------
Example 3:
mother plant cultivation:
(1) in 2018, after the leaves of the peony in autumn are fallen, all branches of the 2-year peony variety 'Luoyang red' on the ground are subjected to stumping treatment, and then winter fertilizer is applied.
(2) And (4) germinating a plurality of new branches at the root neck part of the parent plant in 2019 spring, and performing conventional management in growing seasons. And 9 months in 2019, removing the weak branches from the base, and only keeping robust annual branches without diseases and insect pests, wherein the root system of the mother plant is 3 years and the branch age is only 1 year.
(3) Selecting strong stock plants without diseases and insect pests, and taking seedlings from bare roots.
Oblique planting of a stock plant:
(1) land preparation: soil preparation is completed in advance at the end of 6 months in 2019. The soil of the land parcel is loose and fertile, the organic matter is rich, the drainage is good, the pH is 7.0, the deep ploughing is 50cm, and the prevention and control of the soil diseases and insect pests are managed with the conventional method.
(2) Preparing a planting hole: in 2019, in 9 months, the row spacing is 50cm multiplied by 100cm, the depth is 30cm, the planting holes are inclined towards one side of the row spacing, the included angle between the inclined plane and the horizontal ground is 20 degrees, and the planting holes are arranged in a shape like a Chinese character 'pin'.
Layering the stock plant:
(1) the root system of the bare-root stock plant is placed on the inclined plane of the planting hole to spread the root system, the branch of the stock plant is horizontally fixed on the ground by a U-shaped ground nail, the planting soil is backfilled in the hole layer by layer, and the planting soil is trampled.
(2) The cultivation method is characterized in that loose fertile planting soil is used, organic matters are rich, water drainage is good, planting soil with the pH value of 7.0 is piled to be 40cm high on the ground, all mother plant branches can be covered by the cultivation soil pile, and 2 bamboo poles with the diameter of 1cm are inserted into the soil pile to facilitate ventilation of the branches in the soil pile.
(3) When the soil temperature changes from "day to night freezing", namely above 0 ℃ in the day and when the temperature drops below 0 ℃ at night, the soil temperature is irrigated with enough "frozen water" to facilitate the branches to pass the winter.
Promoting new branches and keeping the base young:
(1) before germination in spring of 2020, commercial organic fertilizer is scattered among each planting row, and the application amount meets the requirements of the specification. After fertilization, the fertilizer is combined with spring irrigation and is turned into soil.
(2) Then, each branch of the mother plant under the soil pile sprouts 3 new branches on average and gradually extends out of the soil surface, and buds on the new branches are removed in time. The hilling pile needs to be reserved so as to be beneficial to the young base of the new branch. Weed is removed at any time, and pest control is carried out regularly.
Injury is caused at the base of the new branch:
(1) in 6 middle of the year 2020, when the area of the leaves of the new branches stops increasing, selecting robust new branches without diseases and insect pests, and digging up a soil heap;
(2) wound making: girdling the base of the branch by using a sharp blade sterilized by 800 times of carbendazim at a position 3cm upwards, wherein the girdling length is 2/3, the girdling width is 1.0cm, and xylem can be seen in depth;
and (3) promoting the root of the new branch:
(1) a cotton ball soaked with NAA500mg/L solution was placed at the girdling site and removed after 10 minutes.
(2) And then restoring the original culture soil pile, covering all the treated branches again, and compacting the branches properly to ensure that each treated branch is in close contact with the soil and the relative water content of the culture soil pile is kept between 60 and 80 percent.
(3) Normal field management, and regular pest control, and the specific measures are the same as the conventional management measures of the peony growing season.
Separating rooted branches/seedlings:
(1) in late 9 months, the soil covering piles are pulled open, a large number of roots grow out of each branch at the binding position of the binding belts or near the girdling position, the rooting rate is 100%, the roots are thick and strong, and secondary roots exist. The average number of the first-level roots is 9.5 per branch, the average root length is 9.4cm, and the average root thickness is 2.1 mm.
(2) Cutting the rooted branch with sharp tool to obtain new seedling. Placing in shade place for planting.
Seedling planting:
(1) the land preparation requirement is the same as that of mother plant planting; the planting holes are 20cm multiplied by 20cm, and the planting row spacing is 20cm multiplied by 20 cm.
(2) And backfilling 5cm of planting soil in the planting holes, placing the seedlings in the center of the planting holes, and backfilling layer by layer while treading. The neck of the seedling root is piled with 10cm high soil to facilitate the winter.
(3) The frozen water is required to be cultivated with the same mother plant, and the management in the next year growth period is the same as the conventional peony seedling management.
Claims (9)
1. A comprehensive method for rapid vegetative propagation of peony is characterized by comprising the following steps:
step one, mother plant cultivation
(1) 1 year ahead, after the autumn peony leaves, the overground parts (namely all branches) of 1-3 year old breeding mother plants are subjected to stumping treatment, and winter fertilizer is applied;
(2) germinating a plurality of new branches at the root neck part of the stock plant in the next year, and performing conventional management in a growing season; removing weak branches from the base in autumn of the same year, and only keeping robust annual branches without diseases and insect pests, wherein the root system of the stock plant is 2-4 years and the branch age is only 1 year;
(3) selecting a strong stock plant without diseases and insect pests, and taking seedlings from bare roots;
step two, obliquely planting the stock plant
(1) Land preparation: at least 1 month in advance; the method comprises the following steps: the soil is loose and fertile, rich in organic matters, good in drainage, with the pH value of 6.5-7.5, and 50cm deep ploughed, and the soil pest control is the same as the conventional management measures;
(2) preparing a planting hole: the row spacing of the plants is 50cm multiplied by 100cm, the depth is 30cm, the planting holes incline towards one side of the row spacing, the included angle between the inclined plane and the horizontal ground is 15-20 degrees, preferably the included angle between the inclined plane and the horizontal ground is 15 degrees, and the planting holes are arranged in a shape like a Chinese character 'pin';
step three, layering the stock plant
(1) Placing the bare-root stock plant into the hole obliquely, placing the root system on the oblique plane to enable the root system to be stretched, horizontally fixing the branch of the stock plant on the ground by using a U-shaped ground nail, backfilling planting soil in the hole layer by layer, and treading tightly;
(2) the method is characterized in that loose fertile planting soil with rich organic matters and good drainage is used, the planting soil with the pH value of 6.5-7.5 is piled up to the height of 30cm on the ground, all branches of a stock plant can be covered by a soil pile, and 1-2 bamboo poles with the diameter of 1cm are inserted into the soil pile to facilitate ventilation of the branches;
(3) when the soil temperature is changed into day and night freezing, namely the temperature is above 0 ℃ in the day and is reduced to below 0 ℃ at night, the frozen water is filled sufficiently to facilitate the branches to pass the winter;
step four, promoting the growth of new branches and keeping the base part rejuvenated
(1) Before germination in spring of the next year, spreading commercially available organic fertilizer (such as decomposed chicken manure or bacterial manure, wherein the fertilizing amount is different according to the selected fertilizer) among each planting row; after fertilization, watering is combined, and the soil is turned into;
(2) then each branch of the mother plant under the soil pile sprouts at least more than 4 new branches and gradually extends out of the soil surface, and if a flower bud exists, the new branch is removed in time; the hilling pile needs to be reserved so as to be beneficial to keeping the base of the new branch young; weeds are removed at any time, and pest control is carried out at regular intervals;
step five, injury is caused at the base of the new branch
(1) In 4-7 months in the year, when the leaf area of the new branch stops increasing, selecting a robust new branch without diseases and insect pests, and digging up a culture soil pile;
(2) most preferably, the wound is a transverse constriction: binding 3-5cm above the base of each branch tightly with a self-locking nylon tie (length 150mm, width 2.7 mm);
(3) the wound is preferably girdling: girdling the layer of the sharp blade sterilized with 800-fold carbendazim: the girdling length is 2/3 branch girth, and xylem is visible deeply; the girdling width is 1.5cm, preferably 1.5 cm;
step six, new branch root promoting treatment
(1) Placing a cotton ball soaked with a rooting promoter at the position of the binding belt or the girdling part for 10 minutes and then removing the cotton ball; most preferably the hormone and concentration is IBA (indolebutyric acid) 1200mg/L, more preferably the hormone and concentration is IBA500mg/L, preferably the hormone and concentration is NAA (naphthylacetic acid) 500 mg/L;
(2) then covering the ripped up hilling pile again, and compacting properly to ensure that each treated branch is in close contact with soil, and keeping the relative water content of the soil in the hilling pile to be 60-80%;
(3) normal field management, and regular pest control, the concrete measures are the same as the conventional management measures of the peony growing season;
seventhly, separating the rooted branches/seedlings
(1) After 3 months, the covering soil pile is pulled open, and a large number of root systems grow out of each branch at the binding position of the binding belt or the position near the girdling position, wherein the root systems are thick and have primary and secondary roots;
(2) cutting the rooted branches by a sharp tool to obtain new seedlings; placing in shade place for planting;
step eight, planting seedlings
(1) The land preparation requirement is the same as that of mother plant planting; the specification and the row spacing of the planting holes are suitable for the root systems of the seedlings;
(2) backfilling 5-10 cm planting soil in the planting hole, placing the seedling in the center of the hole, backfilling layer by layer while treading; the neck of the seedling root is piled up with 10cm high soil to facilitate the winter;
(3) the frozen water is required to be irrigated to the same stock plant, and the management in the next year growth period is the same as the conventional peony seedling management.
2. The method of claim 1, wherein the root system of the parent plant is 2-4 years old and the branch age is only 1 year.
3. A comprehensive method for rapid vegetative propagation of peony according to claim 1, wherein in step two, the planting holes of the mother plant are inclined towards the side with the row spacing, the inclined plane has an angle of 15-20 degrees with the horizontal ground, preferably 15 degrees with the horizontal ground, and the planting holes are arranged in a delta shape.
4. The comprehensive method for rapid vegetative propagation of peony according to claim 1, wherein in the step of three-purpose, the mother plant branch is horizontally fixed on the ground by a U-shaped ground nail, and covered by a 30cm high hilling pile, and 1-2 bamboo poles with the diameter of 1cm are inserted into the hilling pile to facilitate ventilation of the branch.
5. The comprehensive method for rapid vegetative propagation of peony according to claim 1, wherein 3-4 new shoots germinate from the branches of the mother plant under each culture soil heap in spring of four years, and the culture soil heap is retained to facilitate the base of the new shoots to keep young.
6. The comprehensive method for rapid asexual propagation of peony according to claim 1, wherein in step five, the base of each branch is wounded at a position 3cm-5cm upward, and most preferably, the branch is bound tightly with a self-locking nylon tie (150 mm in length and 2.7mm in width) for transverse tying; the girdling layer is preferably formed by using a sharp blade which is sterilized by 800 times of carbendazim, the girdling length is 2/3 the circumference of a branch, the depth is visible in xylem, the girdling width is 1.5cm, and the girdling width is preferably 1.0 cm.
7. A comprehensive method for rapid vegetative propagation of peony according to claim 1, wherein in step six, cotton balls soaked with rooting promoters are placed at the position where the tie is tightened or girdled, and removed after 10 minutes; most preferably, the rooting promoter is IBA (indolebutyric acid) with a concentration of 1200mg/L, more preferably IBA (indolebutyric acid) with a concentration of 500mg/L, and the rooting promoter is NAA (naphthylacetic acid) with a concentration of 500 mg/L.
8. The comprehensive method for rapid vegetative propagation of peony according to claim 1, wherein the time for injury treatment at the base of the shoot is the period of vigorous growth of peony in one year.
9. A comprehensive method for rapid vegetative propagation of peony according to claim 1, wherein the rooting rate of the seedling after 3 months of treatment in the fifth and sixth steps can reach 100%, the root system is developed and strong and has first and second roots.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110183398.7A CN112913488B (en) | 2021-02-10 | 2021-02-10 | Comprehensive method for rapid asexual propagation of peony |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110183398.7A CN112913488B (en) | 2021-02-10 | 2021-02-10 | Comprehensive method for rapid asexual propagation of peony |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112913488A true CN112913488A (en) | 2021-06-08 |
| CN112913488B CN112913488B (en) | 2023-09-08 |
Family
ID=76171515
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110183398.7A Active CN112913488B (en) | 2021-02-10 | 2021-02-10 | Comprehensive method for rapid asexual propagation of peony |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112913488B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114910507A (en) * | 2022-05-11 | 2022-08-16 | 中国科学院西北生态环境资源研究院 | Soil sample freezing test method and related equipment |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201037077A (en) * | 2009-03-16 | 2010-10-16 | Nat Inst Of Agrobio Sciences | Method for preventing or inhibiting infection of plant by microorganisms, and plant having resistance against infection by microorganisms |
| RU2411716C1 (en) * | 2009-07-10 | 2011-02-20 | Федеральное государственное образовательное учреждение высшего профессионального образования "Московский государственный университет природообустройства" Министерства сельского хозяйства РФ | Method to grow scion-rooted grape planting stock and machine for its realisation |
| CN102640635A (en) * | 2012-03-31 | 2012-08-22 | 湖北武当生物医药科技有限公司 | Seedling raising and planting method of tree honeysuckle |
| CN103535271A (en) * | 2013-10-24 | 2014-01-29 | 遵义市红城农业开发有限公司 | Camellia changii variety breeding method |
| CN103621399A (en) * | 2012-08-20 | 2014-03-12 | 阎立燕 | Peony tissue culture method |
| CN105052512A (en) * | 2015-08-27 | 2015-11-18 | 南京绿宙油用牡丹研究所有限公司 | Method for promoting oil-utilized peony seedling sprout germination |
| CN106234216A (en) * | 2016-07-28 | 2016-12-21 | 青川县青源林农产品开发有限责任公司 | A kind of oil Peony growing method |
| CN107087526A (en) * | 2017-06-28 | 2017-08-25 | 佛山市幻实科技有限公司 | Camellia nitidissima bending pruning propagation method |
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
| RU2666182C1 (en) * | 2017-09-18 | 2018-09-06 | Федеральное государственное автономное образовательное учреждение высшего образования "Крымский федеральный университет имени В.И. Вернадского" | Method for growing two-year-young plants of fruit crops with a crown |
| CN109315158A (en) * | 2018-09-29 | 2019-02-12 | 十堰中盈生态农业发展有限公司 | Northwest of Hubei Province oil tree peony fast asexual propagation technology |
| CN109349055A (en) * | 2018-11-15 | 2019-02-19 | 阜阳市新丰种业有限公司 | A kind of High-cold regions oil tree-peony transplantation implantation methods |
| CN110100633A (en) * | 2019-04-22 | 2019-08-09 | 中国林业科学研究院热带林业研究所 | A kind of Phoebe bournei overhead layering method for culturing seedlings |
| CN110122153A (en) * | 2019-05-30 | 2019-08-16 | 江苏文胜科技股份有限公司 | A kind of oil tree peony implantation methods |
| US20200128756A1 (en) * | 2018-10-24 | 2020-04-30 | Giang Yuke Ming | Method of planting grafted teak (tectona grandis) trees |
-
2021
- 2021-02-10 CN CN202110183398.7A patent/CN112913488B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201037077A (en) * | 2009-03-16 | 2010-10-16 | Nat Inst Of Agrobio Sciences | Method for preventing or inhibiting infection of plant by microorganisms, and plant having resistance against infection by microorganisms |
| RU2411716C1 (en) * | 2009-07-10 | 2011-02-20 | Федеральное государственное образовательное учреждение высшего профессионального образования "Московский государственный университет природообустройства" Министерства сельского хозяйства РФ | Method to grow scion-rooted grape planting stock and machine for its realisation |
| CN102640635A (en) * | 2012-03-31 | 2012-08-22 | 湖北武当生物医药科技有限公司 | Seedling raising and planting method of tree honeysuckle |
| CN103621399A (en) * | 2012-08-20 | 2014-03-12 | 阎立燕 | Peony tissue culture method |
| CN103535271A (en) * | 2013-10-24 | 2014-01-29 | 遵义市红城农业开发有限公司 | Camellia changii variety breeding method |
| CN105052512A (en) * | 2015-08-27 | 2015-11-18 | 南京绿宙油用牡丹研究所有限公司 | Method for promoting oil-utilized peony seedling sprout germination |
| CN106234216A (en) * | 2016-07-28 | 2016-12-21 | 青川县青源林农产品开发有限责任公司 | A kind of oil Peony growing method |
| CN107087526A (en) * | 2017-06-28 | 2017-08-25 | 佛山市幻实科技有限公司 | Camellia nitidissima bending pruning propagation method |
| RU2666182C1 (en) * | 2017-09-18 | 2018-09-06 | Федеральное государственное автономное образовательное учреждение высшего образования "Крымский федеральный университет имени В.И. Вернадского" | Method for growing two-year-young plants of fruit crops with a crown |
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
| CN109315158A (en) * | 2018-09-29 | 2019-02-12 | 十堰中盈生态农业发展有限公司 | Northwest of Hubei Province oil tree peony fast asexual propagation technology |
| US20200128756A1 (en) * | 2018-10-24 | 2020-04-30 | Giang Yuke Ming | Method of planting grafted teak (tectona grandis) trees |
| CN109349055A (en) * | 2018-11-15 | 2019-02-19 | 阜阳市新丰种业有限公司 | A kind of High-cold regions oil tree-peony transplantation implantation methods |
| CN110100633A (en) * | 2019-04-22 | 2019-08-09 | 中国林业科学研究院热带林业研究所 | A kind of Phoebe bournei overhead layering method for culturing seedlings |
| CN110122153A (en) * | 2019-05-30 | 2019-08-16 | 江苏文胜科技股份有限公司 | A kind of oil tree peony implantation methods |
Non-Patent Citations (1)
| Title |
|---|
| 易米平;张日清;董凤祥;王贵禧;翟学杰;: "榛子无性繁殖技术研究的现状与趋势", 经济林研究, no. 01 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114910507A (en) * | 2022-05-11 | 2022-08-16 | 中国科学院西北生态环境资源研究院 | Soil sample freezing test method and related equipment |
| US12031971B2 (en) | 2022-05-11 | 2024-07-09 | Northwest Institute Of Eco-Environment And Resources, Chinese Academy Of Sciences | Method for testing frost susceptibility of soils and associated apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112913488B (en) | 2023-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109220465B (en) | Method for cultivating and trimming pepper trees | |
| CN1922987A (en) | Multiple-ear rock lithocarpus glaber dwarfing rapid reproduction method | |
| CN106900461B (en) | Bionic compound operation method of vine tea, woody oil crops and mountain rice | |
| CN105309157A (en) | Cultivation method of ginkgo trees | |
| CN108541469B (en) | Winter grafting rapid seedling method for dwarf apple interstock | |
| CN113273422A (en) | Olive planting method | |
| CN106258726B (en) | Method for improving acre yield of momordica grosvenori | |
| CN112772081A (en) | Seedling cultivation method for agricultural planting based on controlled release fertilizer | |
| CN112913488B (en) | Comprehensive method for rapid asexual propagation of peony | |
| CN113973600B (en) | Method for grafting and cultivating pecan with xylem bud by taking square bark | |
| CN109548553A (en) | A kind of facility cultivation method of control grape young sprout growth | |
| CN107439200A (en) | A kind of implantation methods of cotton interplanting potato | |
| CN114431013A (en) | Planting method of Venus golden apple trees | |
| CN109937722B (en) | Method for rapidly propagating Chinese lizardtail ground stems | |
| CN109105122B (en) | Method for quickly building garden on big walnut tree and grafting method | |
| CN112913619A (en) | Method for cultivating spatholobus stem in short period | |
| CN111820027A (en) | Grape cold shed early-maturing cultivation method | |
| CN111903414A (en) | Method for artificially breeding wild Machilus kwangtungensis into landscaping tree species | |
| CN105706748A (en) | Cedrela sinensis and cedrela toona grafting propagation method | |
| CN106613689B (en) | Method for rapidly breeding paeonia suffruticosa | |
| CN110741868A (en) | Seedling planting method | |
| CN110896769A (en) | Kiwi fruit non-habitat planting method | |
| CN115486312B (en) | Two-stage seedling raising method for apocarya grafted seedlings in nutrition pot | |
| CN108834896A (en) | A kind of fructus lycii implantation methods based on peaceful Qi 6 and peaceful Qi 8 | |
| CN113455265B (en) | Method for cultivating golden camellia seedlings with concave veins |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |