CN103535271A - Camellia changii variety breeding method - Google Patents
Camellia changii variety breeding method Download PDFInfo
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- CN103535271A CN103535271A CN201310505967.0A CN201310505967A CN103535271A CN 103535271 A CN103535271 A CN 103535271A CN 201310505967 A CN201310505967 A CN 201310505967A CN 103535271 A CN103535271 A CN 103535271A
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Abstract
The invention discloses a camellia changii variety breeding method which comprises the following steps of selecting budding flowers by taking fragrant flowers with rich flower colors and flower types as breeding aims in a camellia changii male parent blooming season, poking petals, quickly removing all stamens, smearing pollens to be pollinated on stigmas, folding an adhesive plaster in two to completely cover the stigmas for controlled pollination to realize the controlled pollination of theaceae plants, and performing seed breeding of hybrids under an indoor control condition. The variety of camellia changii is small, the number of female parents is small, and the breeding potential is low. By a tissue culture technology disclosed by the invention, the problems can be well solved; rare and precious resources, namely the camellia changii, can be protectively used; the problem that the camellia changii seedlings are scarce can be alleviated.
Description
Technical field
The present invention relates to a kind of camellia azalea varieties breeding method, belong to Camellia seedling growing process field.
Background technology
Camellia azalea belongs to evergreen shrubs to dungarunga.High 1~2m, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 5~10cm, tree body is short crown, and camellia azalea branches and leaves are close, compact.Bark taupe, branch is smooth, and the tender tip is red, the long 8~12cm of leaf, the micro-point in two ends, obovate, keratin, vein is not obvious, abundant, light is dark green, and edge-smoothing, does not ftracture, petiole is short, and variation is little, and petal is long and narrow, and filigree is red, flower pesticide is golden yellow, and Hua Jing is more than 8 centimetres, although single-lobe, the close life of flower, whole plentiful, perpetual bloom is continuous, even if at temperature the summer up to 38 ℃, also still safflower is completely set; Begin mid-April to spend, full-bloom stage is 7-September, continues to February next year.
Camellia azalea is because its profile utmost point is as cuckoo, and essence is but camellia, so gain the name.Cultivation name is called " azalea tea " or " cuckoo tea ".Camellia azalea is an extremely rare camellia kind ,You“ vegetative kingdom giant panda " title, endangered camellia azalea once.The existing proud plum strength of character of common camellia, has again tree peony gorgeous, is exactly the Woody flower ,You“ world famous flower that is rich in great reputation since ancient times " good name.And the pretty and dazzling of camellia azalea goes even farther than common camellia.
The flower of camellia azalea is the same with cuckoo, has 5 petals, the shape that petal is stretched out also the utmost point as the appearance of azalea, but flower type larger than azalea.Cereiform bud top is raw or armpit is raw, and bud is very large, and pattern is cerise, and petal is long and narrow, filigree white, and flower pesticide is golden yellow, and the diameter of flower is more than 10 centimetres.Although be single-lobe, the close life of flower, whole plentiful, perpetual bloom is continuous, even if in the winter time, also still safflower is completely set.
Yet planting conditions and the production technology of camellia azalea are all had relatively high expectations, and product is very rare.At present, its survival rate of seedling is low, and crop cycle is long.
Summary of the invention
The object of the invention is in order to overcome deficiency of the prior art, thereby a kind of camellia azalea varieties breeding method is provided.
The present invention adopts following technical scheme:
A kind of camellia azalea varieties breeding method, the method step is as follows: take enrich pattern, flower type, fragrant flower are breeding objective, in camellia azalea male parent, bloom season, select the flower that a bud just ready to burst, push petal aside, reject rapidly whole stamens, on column cap, smear pollen to be awarded, by encasing the column cap of controlled pollination after adhesive plaster doubling completely, realize plant of theaceae controlled pollination, indoor controlled condition is carried out the seed seedling-raising of hybrid.
A varieties breeding method, the method step is as follows: by spontaneous pollination seed seedling, artificial hybridization seed seedling Phenotypic Observation, carry out early stage defect individual selection, adopt tissue culture technology to carry out the breeding of defect individual.
Adopt the beneficial effect of technique scheme to be:
A camellia azalea kind group of mean people of the present invention, maternal few, reproduction rate is not high.For this reason, Sterile culture is difficult to expand at short notice its population quantity, therefore with Sterile culture, is difficult to method and develops this fine germplasm resources.And the tissue culture technique that this project adopts can address this problem well: not only can realize this rare resources protectiveness utilization of camellia azalea, can also alleviate the rare problem of camellia azalea nursery stock.First tackle existing resource and carry out field on-site survey on the spot, collection, set up resource garden.Next adopts base portion to urge to sprout, and adult elite stand is carried out to childrenization rejuvenation processing, adopts the approach such as the generation of clump bud, organ generation, the generation of body embryo and anther culture, and system is carried out the tissue cultivating to camellia azalea; By the screening of minimal medium, hormone kind and culture environment condition, optimize the condition of culture of different cultivation approach and different cultivation stages.
Embodiment
Below the present invention is described in further detail:
Embodiment mono-:
A kind of camellia azalea varieties breeding method, the method step is as follows: take enrich pattern, flower type, fragrant flower are breeding objective, in camellia azalea male parent, bloom season, select the flower that a bud just ready to burst, push petal aside, reject rapidly whole stamens, on column cap, smear pollen to be awarded, by encasing the column cap of controlled pollination after adhesive plaster doubling completely, realize plant of theaceae controlled pollination, indoor controlled condition is carried out the seed seedling-raising of hybrid.
The present invention take enrich pattern, flower type, fragrant flower are breeding objective, choose reasonable hybrid strain.According to male parent camellia flowering habit, determine best pollen acquisition time; Utilize low temperature drying to process and optimize pollen storage condition; Adopt the distinct methods such as bagging to optimize hybridization technique, by increasing the raisings such as Pollination Times, pollination amount, be hybridized to power; Utilize tissue culture technique to carry out the redemption of hybrid training embryo, adopt indoor controlled condition to carry out the seed seedling-raising of hybrid, improve hybrid and obtain seedling rate.
Embodiment bis-:
A varieties breeding method, the method step is as follows: by spontaneous pollination seed seedling, artificial hybridization seed seedling Phenotypic Observation, carry out early stage defect individual selection, adopt tissue culture technology to carry out the breeding of defect individual.
A camellia azalea kind group of mean people of the present invention, maternal few, reproduction rate is not high.For this reason, Sterile culture is difficult to expand at short notice its population quantity, therefore with Sterile culture, is difficult to method and develops this fine germplasm resources.And the tissue culture technique that this project adopts can address this problem well: not only can realize this rare resources protectiveness utilization of camellia azalea, can also alleviate the rare problem of camellia azalea nursery stock.First tackle existing resource and carry out field on-site survey on the spot, collection, set up resource garden.Next adopts base portion to urge to sprout, and adult elite stand is carried out to childrenization rejuvenation processing, adopts the approach such as the generation of clump bud, organ generation, the generation of body embryo and anther culture, and system is carried out the tissue cultivating to camellia azalea; By the screening of minimal medium, hormone kind and culture environment condition, optimize the condition of culture of different cultivation approach and different cultivation stages.
Claims (2)
1. a camellia azalea varieties breeding method, it is characterized in that: the method step is as follows: take enrich pattern, flower type, fragrant flower are breeding objective, in camellia azalea male parent, bloom season, select the flower that a bud just ready to burst, push petal aside, reject rapidly whole stamens, on column cap, smear pollen to be awarded, by encasing the column cap of controlled pollination after adhesive plaster doubling completely, realize plant of theaceae controlled pollination, indoor controlled condition is carried out the seed seedling-raising of hybrid.
2. a camellia azalea varieties breeding method, is characterized in that: the method step is as follows: by spontaneous pollination seed seedling, artificial hybridization seed seedling Phenotypic Observation, carry out early stage individual choice, adopt tissue culture technology to carry out individual breeding.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103891601A (en) * | 2014-03-27 | 2014-07-02 | 中国农业科学院茶叶研究所 | Artificial hybridization type petal pollination method for tea tree |
CN112913488A (en) * | 2021-02-10 | 2021-06-08 | 北京林业大学 | Comprehensive method for rapid vegetative propagation of peony |
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JP2001192310A (en) * | 2000-01-12 | 2001-07-17 | Mitsui Chemicals Inc | Method for promoting absorption of calcium ion from surface of plant |
CN1985580A (en) * | 2006-12-18 | 2007-06-27 | 江苏阳光生态农林开发股份有限公司 | Fast tissue culture propagation process for azalea and camellia |
CN102960172A (en) * | 2012-12-10 | 2013-03-13 | 广西壮族自治区林业科学研究院 | Short-spike and root-grafting rapid propagation method for camellia azalea |
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2013
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2001192310A (en) * | 2000-01-12 | 2001-07-17 | Mitsui Chemicals Inc | Method for promoting absorption of calcium ion from surface of plant |
CN1985580A (en) * | 2006-12-18 | 2007-06-27 | 江苏阳光生态农林开发股份有限公司 | Fast tissue culture propagation process for azalea and camellia |
CN102960172A (en) * | 2012-12-10 | 2013-03-13 | 广西壮族自治区林业科学研究院 | Short-spike and root-grafting rapid propagation method for camellia azalea |
Non-Patent Citations (2)
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103891601A (en) * | 2014-03-27 | 2014-07-02 | 中国农业科学院茶叶研究所 | Artificial hybridization type petal pollination method for tea tree |
CN103891601B (en) * | 2014-03-27 | 2016-04-06 | 中国农业科学院茶叶研究所 | Tea tree artificial hybridization petal pollination method |
CN112913488A (en) * | 2021-02-10 | 2021-06-08 | 北京林业大学 | Comprehensive method for rapid vegetative propagation of peony |
CN112913488B (en) * | 2021-02-10 | 2023-09-08 | 北京林业大学 | Comprehensive method for rapid asexual propagation of peony |
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Application publication date: 20140129 |