CN110100633A - A kind of Phoebe bournei overhead layering method for culturing seedlings - Google Patents

A kind of Phoebe bournei overhead layering method for culturing seedlings Download PDF

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Publication number
CN110100633A
CN110100633A CN201910323799.0A CN201910323799A CN110100633A CN 110100633 A CN110100633 A CN 110100633A CN 201910323799 A CN201910323799 A CN 201910323799A CN 110100633 A CN110100633 A CN 110100633A
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China
Prior art keywords
press strip
band girdle
culturing seedlings
phoebe bournei
root
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CN201910323799.0A
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Chinese (zh)
Inventor
李荣生
尹光天
杨锦昌
邹文涛
王旭
余纽
郑雨盼
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Research Institute of Tropical Forestry of Chinese Academy of Forestry
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Research Institute of Tropical Forestry of Chinese Academy of Forestry
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Application filed by Research Institute of Tropical Forestry of Chinese Academy of Forestry filed Critical Research Institute of Tropical Forestry of Chinese Academy of Forestry
Priority to CN201910323799.0A priority Critical patent/CN110100633A/en
Publication of CN110100633A publication Critical patent/CN110100633A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G23/00Forestry
    • A01G23/02Transplanting, uprooting, felling or delimbing trees
    • A01G23/04Transplanting trees; Devices for grasping the root ball, e.g. stump forceps; Wrappings or packages for transporting trees
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G3/00Cutting implements specially adapted for horticultural purposes; Delimbing standing trees
    • A01G3/08Other tools for pruning, branching or delimbing standing trees
    • A01G3/081Manual cutting tools not otherwise provided for, e.g. pruning hooks
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of Phoebe bournei overhead layering method for culturing seedlings.The Phoebe bournei overhead layering method for culturing seedlings includes elite stand selection, branch selection, makes wound, promoting root growth processing, overhead layering processing, transplanting, transplanting and management.Had the effect that using method for culturing seedlings of the invention 1) take root it is high-efficient, be 44%;2) management less, without management during press strip, has had root system after transplanting, it is only necessary to watering and shade, and the management phase only needs 1 year;3) well developed root system: after press strip 3 months, number of taking root is up to 29, a length of 12.2cm of maximum root, and maximum root thickness are 4.85mm;4) transplanting success is high: press strip transplanting success is up to 85%;5) outplanting is fast: after press strip grows 1 year in nursery, the length is 51 ± 3cm, reach the standard of level-one seedling and second level seedling as defined in national forestry professional standard " nanmu Cultivating techniques regulation ".

Description

A kind of Phoebe bournei overhead layering method for culturing seedlings
Technical field
The invention belongs to breeding plant technical fields, and in particular to a kind of Phoebe bournei overhead layering method for culturing seedlings.
Background technique
Phoebe bournei (Phoebe bournei) is Lauraceae (Lauraceae) Phoebe (Phoebe) evergreen megaphanerophyte, is China Asia The precious material in native soil and ornamental tree species that torrid areas is cultivated extensively are traditional name wood --- one of original tree species of nanmu;Its The grain of wood is fine and smooth, be not easy to warp and fracture, rotproofness is strong, handling ease, is the excellent natural ring for making furniture, board and decorative house Protect material;Big tree grandness is tall and straight, crown type is beautiful, the service life is permanent for its, is usually used in four sides and afforestation.
Nursery of nourishing and generating refer to by vegetable nutritorium cultivate nursery stock breeding and seedling in the way of, be cultivated in forestry it is excellent The main method for setting plant division is the key link of forest clonal seed orchard construction, is the important way that Forest Tree New Varieties are cultivated.
The Phoebe bournei clearly reported nursery of nourishing and generating at present has 2 kinds of methods of cuttage and seedling culture and tissue culture.Wherein, cuttage The step of nursery is the most seedling raising manners of nourishing and generating of Phoebe bournei research report, cutting propagation nursery has the selection of cuttage period, skewer Management etc. after slotting matrix preparation, fringe bar acquisition, fringe processed, cuttings sterilize and (or) dip in root-growing agent, cuttage and insert.Difference report is open Cuttage raise seedling technique used in matrix and root-growing agent it is different, cuttings is confined to that nursery stock stem section, rugosity is thin for starting, length is short, after inserting Manage more, the management time is longer, and published have been reported that the speed of growth of aerial part and is not out of the garden the time, can Also imperfect to say, the current-year branch cuttage root-taking of the above trees raw for 5 years is ineffective.
Phoebe bournei tissue culture only have at present 1 research report (Qu Fenxia, it is old to deposit and .2010. Phoebe bournei group culturation rapid propagating technology is ground Study carefully forestry practical technique, (11): 7-9), this report thinks preferably to intercept stem section in spring and summer field acquisition Phoebe bournei coppice shoot and make For explant, aseptic culture medium is sterilized and is inoculated with 0.2% potassium permanganate, and differentiation and proliferation use MS+6-BA2.0mgL- 1+NAA01 5mg·L-1Culture medium is taken root using 1/2MS+IBA0.5mgL-1Culture medium, obtain preferable breeding coefficient and Rooting rate.Although reporting hardening and transplanting work, without reporting subsequent growth and being out of the garden.Though in conclusion the technology To set coppice shoot greatly as explant, but tissue culture propagation seedling-raising technique is still imperfect continuous, and the device is complicated needed for tissue culture, tissue culture process without Bacterium requires high, culture medium to prepare complicated, heavy workload, and incubation need to manually provide illumination, at high cost.
In conclusion Phoebe bournei nourishes and generates nursery based on cuttage and seedling culture at present, but it is suitable for nursery stock stem sections, to 5 years The current-year branch rooting rate of the raw above trees is low, and entire seedling raising process is imperfect.Though it is outer that tissue culture, which can set greatly coppice shoot, Implant, whole flow process seem completely, but the time of links and overall reproductive coefficient are unclear, equipment needed for cultivating process compared with More, sterility requirements are unknown compared with the growing state after tight, hardening and transplanting, therefore still imperfect continuous.To sum up, existing Fujian Nanmu cuttage and seedling culture and 2 kinds of seedling-raising techniques of nourishing and generating of tissue culture are still imperfect, it is difficult to the Phoebe bournei select tree point of competent Seedling selection Strain is cultivated, clonal seed orchard is built and the requirement of rearing new variety.
Summary of the invention
It is an object of the invention to overcome the shortcomings of the prior art, a kind of Phoebe bournei overhead layering method for culturing seedlings is provided.
Phoebe bournei overhead layering method for culturing seedlings of the invention, comprising the following steps:
(1) overhead layering handle: annual 3-9 month, using elite stand branch base diameter for 1.0-2.0cm branch as Press strip finds cyclic annular bud scar in selected branch, carries out band girdle in bud scar lower part, form band girdle mouth, wrapped up with Forest floor soil Firmly band girdle mouth;
(2) transplant: during press strip, whether observation press strip takes root, and the press strip after taking root is cut, trimming blade, by press strip It plants in the container equipped with matrix, irrigates root water, the sunshade net of 60-80% is put up on seedbed, carry out transplanting and management
Described carries out band girdle in bud scar lower part, and forming band girdle mouth is the progress band girdle at the 2cm of bud scar lower part, is formed wide The band girdle mouth of 1cm
The step (1) further includes the steps that ABT rooting powder solution being applied to band girdle mouth.
The ABT rooting powder solution concentration is 1000mgL-1, solvent is water.
Described wraps band girdle mouth with Forest floor soil specifically: by 160-180mL expendable transparent plastic cup side It cuts off, cuts the mouth of size suitable with branch band girdle mouth diameter in bottom of a cup, cover upper branch in band girdle mouth lower edge, with transparent adhesive tape by cup Wall shearing line reseals;Forest floor soil is dug, compacting in cup is packed into, makes branch in cup center or upside, uses transparent adhesive tape Band seals rim of a cup, slightly stationary point gap, and rainwater is facilitated to flow into.
The elite stand is the Phoebe bournei treelet of the 6-9 life of robust growth, no disease and pests harm.
The container equipped with matrix is the container of diameter 15cm, high 15cm, and matrix is general soil.
The transplanting and management is watering 1 time daily, keeps ground moistening.
Using the Phoebe bournei that Phoebe bournei overhead layering method for culturing seedlings of the invention obtains take root press strip grown in nursery 1 year after just Can be out of the garden afforestation.
The beneficial effects of the present invention are:
(1) press strip locality specific is taken root high-efficient: press strip position is located at the 2cm of bud scar lower part, rooting rate 44%;Bud Then rooting rate is only 19% for the control of press strip in the middle part of trace.
(2) band girdle makes that hurt effect good: band girdle rooting rate is up to 19% in the middle part of adjacent bud scar, and it is then 0 that middle part, which scratches rooting rate,. Band girdle press strip rooting rate is 44% at the 2cm of bud scar lower part, and compares scratch rooting rate and there was only 2%.
(3) it manages less: without management during press strip, having had root system after transplanting, it is only necessary to water and shade, and the management phase only needs 1 year.Cuttage and seedling culture needs to cover film and shading net after cuttage, brings inconvenience to watering, and the growth of skewer item is slow, needs 2 years 20cm can be grown to.
(4) well developed root system: after press strip 3 months, number of taking root is up to 29, a length of 12.2cm of maximum root, and maximum root thickness are 4.85mm, and cuttage and seedling culture is taken root number maximum only 4, longest is only 5.84cm, most slightly only 1.5mm (Wang Dongguang etc., it is different The influence that matrix and season take root to Phoebe bournei shoot cutting, tropical crops journal, 2013,34 (8): 1458-1462).
(5) transplanting success is high: press strip is just transplanted after taking root, therefore the cuttings than not having root has higher transplanting survival Rate.According to investigations, press strip transplanting success is up to 85.7%, and the skewer fringe survival rate about 50% of cuttage and seedling culture.
(6) outplanting is fast: after press strip grows 1 year in nursery, the length is 51 ± 3cm, reach national forestry industry The standard of level-one seedling and second level seedling as defined in standard " nanmu Cultivating techniques regulation ".In contrast, the curtailment of cuttage seeding 15cm, far below 25cm as defined in standard.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
1 Shaoguan City of Guangdong Province Yuechang City Longshan Forest Farm Phoebe bournei select tree overhead layering nursery of embodiment
1, elite stand selects: 2017 3 and July, in Phoebe bournei, 7 years non-hibernating eggs source family trial Lin Zhongxuans select robust growth, nothing The Phoebe bournei treelet of pest and disease damage.
2, branch selects: selecting branch base diameter is the branch of 1.0-2.0cm as press strip.
3, it makes wound: finding cyclic annular bud scar in selected branch, clamp branch with vice at 2cm below the bud scar, then Right and left rings turn that the bark clamped is made to fall off, the band girdle mouth of formation width about 1.0cm or so, must shave cortex and forming layer.Control Processing are as follows: a, the band girdle in the middle part of adjacent bud scar, the band girdle mouth of formation width about 1.0cm or so;B, it is scratched in the middle part of adjacent bud scar; C, it is scratched at the 2cm of bud scar lower part.
4, promoting root growth is handled: dipping 1000mgL with writing brush-1ABT1 rooting powder solution (water is solvent) to band girdle mouth or Wound is scraped to be smeared.
5, it press strip: uses the expendable transparent plastic cup of 180mL as lapping, cup side is cut off, in bottom of a cup The mouth for making the suitable size of wound diameter with branch is cut, upper branch is covered in band girdle mouth or wound lower edge, is cut wall of cup with transparent adhesive tape It bursts at the seams and reseals;Forest floor soil is dug, compacting in cup is packed into, makes branch in cup center or upside.It is sealed with adhesive tape Firmly rim of a cup, slightly stationary point gap, facilitate rainwater to flow into.
6, transplant: check press strip after press strip 3 months, through wall of cup observation press strip whether take root, by the press strip after taking root from Bottom of cups is cut, and is taken back nursery, is peelled off the plastic cup of press strip base portion, suitably cuts off some blades, by press strip plantation to diameter The container equipped with matrix of 15cm or so, high 15cm or so, the matrix are general soil, are then placed on seedbed, pour Saturating root water puts up 60% sunshade net on seedbed.Rooting rate result is as follows: band girdle control group rooting rate in the middle part of adjacent bud scar Up to 19%, and it is then 0 that rooting rate is scratched in the middle part of adjacent bud scar.The processing group press strip rooting rate of band girdle is at the 2cm of bud scar lower part 44%, and compare bud scar lower part 2cm scratch rooting rate and there was only 2%.
After press strip 3 months, number of taking root is up to 29, a length of 12.2cm of maximum root, and maximum root thickness are 4.85mm, and cuttage Seedling cultivation and rooting number is up to 4, up to 5.84cm.
7, transplanting and management: watering 1 time daily keeps ground moistening.
8, nursery effect is investigated: being investigated discovery after transplanting 1 year, is transplanted 91, survive 78, press strip transplanting success is up to 85.7%, and the skewer fringe survival rate about 50% of cuttage and seedling culture.After press strip grows 1 year in nursery, the length is 51 ± 3cm, reach To the standard of level-one seedling and second level seedling as defined in national forestry professional standard " nanmu Cultivating techniques regulation ".In contrast, cuttage The curtailment 15cm of seedling, far below 25cm as defined in standard.
2 Shaoguan City of Guangdong Province Pingyuan County Phoebe bournei treelet overhead layering nursery of embodiment
1, elite stand selects: part of in September, 2018 selects robust growth, 6 years raw Phoebe bournei treelets of no disease and pests harm are elite stand.
2, branch selects: selecting branch base diameter is the primary branches of 1.5cm or so as press strip.
3, it makes wound: finding cyclic annular bud scar in selected branch, clamp branch with vice at 2cm under bud scar, it is then left Right ring turns that the bark clamped is made to fall off, the band girdle mouth of formation width about 1.0cm or so, must shave cortex and forming layer.
4, it press strip: uses the expendable transparent plastic cup of 160mL as lapping, cup side is cut off, in bottom of a cup The mouth for making the suitable size of wound diameter with branch is cut, covers upper branch in band girdle mouth lower edge, with transparent adhesive tape by wall of cup shearing line weight Novel sealing;Forest floor soil is dug, compacting in cup is packed into, makes branch in cup center or upside.Rim of a cup is sealed with adhesive tape, Slightly stationary point gap, facilitates rainwater to flow into.
5, transplant: check press strip after press strip 3 months, through wall of cup observation press strip whether take root, by the press strip after taking root from Bottom of cups is cut, and is taken back nursery, is peelled off the plastic cup of press strip base portion, suitably cuts off some blades, by press strip plantation to diameter The container equipped with matrix of 15cm or so, high 15cm or so, the matrix are general soil, are then placed on seedbed, pour Saturating root water puts up 80% sunshade net on seedbed.Press strip rooting rate is 44% or more.
6, transplanting and management: watering 1 time daily keeps ground moistening.
7, nursery effect is investigated: being investigated discovery after transplanting 3 months, is transplanted 589, survive 499, transplanting success 84.7%.After press strip grows 1 year in nursery, the length is 51 ± 3cm, and reaching national forestry professional standard, " nanmu cultivates skill Art regulation " as defined in level-one seedling and second level seedling standard.In contrast, the curtailment 15cm of cuttage seeding is advised far below standard Fixed 25cm.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change It also should be regarded as protection scope of the present invention into retouching.

Claims (8)

1. a kind of Phoebe bournei overhead layering method for culturing seedlings, which comprises the following steps:
(1) overhead layering handle: annual 3-9 month, using elite stand branch base diameter for 1.0-2.0cm branch as press Item finds cyclic annular bud scar in selected branch, carries out band girdle in bud scar lower part, form band girdle mouth, wrapped with Forest floor soil Band girdle mouth;
(2) transplant: during press strip, whether observation press strip takes root, and the press strip after taking root is cut, and trimming blade plants press strip To in the container equipped with matrix, root water is irrigated, the sunshade net of 60-80% is put up on seedbed, carries out transplanting and management.
2. Phoebe bournei overhead layering method for culturing seedlings according to claim 1, which is characterized in that described to be carried out in bud scar lower part Band girdle, forming band girdle mouth is the progress band girdle at the 2cm of bud scar lower part, forms the band girdle mouth of width 1cm.
3. Phoebe bournei overhead layering method for culturing seedlings according to claim 1 or 2, which is characterized in that the step (1) further includes The step of ABT rooting powder solution is applied to band girdle mouth.
4. Phoebe bournei overhead layering method for culturing seedlings according to claim 3, which is characterized in that the ABT rooting powder solution Concentration is 1000mgL-1
5. Phoebe bournei overhead layering method for culturing seedlings according to claim 1, which is characterized in that described uses Forest floor Tu Bao Wrapping band girdle mouth is to cut off the expendable transparent plastic cup side of 160-180mL, is cut and branch band girdle mouth diameter in bottom of a cup The mouth of suitable size covers upper branch in band girdle mouth lower edge, is resealed wall of cup shearing line with transparent adhesive tape;Dig Forest floor Soil is packed into compacting in cup, makes branch in cup center or upside, seals rim of a cup, slightly stationary point gap with adhesive tape, facilitate rainwater It flows into.
6. Phoebe bournei overhead layering method for culturing seedlings according to claim 1, which is characterized in that the elite stand is that growth is strong Strong, no disease and pests harm 6-9 raw Phoebe bournei treelet.
7. Phoebe bournei overhead layering method for culturing seedlings according to claim 1, which is characterized in that the container equipped with matrix For the container of diameter 15cm, high 15cm, matrix is soil.
8. Phoebe bournei overhead layering method for culturing seedlings according to claim 1, which is characterized in that the transplanting and management is daily Watering 1 time keeps ground moistening.
CN201910323799.0A 2019-04-22 2019-04-22 A kind of Phoebe bournei overhead layering method for culturing seedlings Pending CN110100633A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112913488A (en) * 2021-02-10 2021-06-08 北京林业大学 Comprehensive method for rapid vegetative propagation of peony

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CN104054501A (en) * 2014-07-15 2014-09-24 广西壮族自治区林业科学研究院 Phoebe bournei cutting propagation method
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Publication number Priority date Publication date Assignee Title
CN112913488A (en) * 2021-02-10 2021-06-08 北京林业大学 Comprehensive method for rapid vegetative propagation of peony
CN112913488B (en) * 2021-02-10 2023-09-08 北京林业大学 Comprehensive method for rapid asexual propagation of peony

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Application publication date: 20190809