CN101336589A - Cuttage cultivation method for dendrobium officinale - Google Patents
Cuttage cultivation method for dendrobium officinale Download PDFInfo
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- CN101336589A CN101336589A CNA2008100217906A CN200810021790A CN101336589A CN 101336589 A CN101336589 A CN 101336589A CN A2008100217906 A CNA2008100217906 A CN A2008100217906A CN 200810021790 A CN200810021790 A CN 200810021790A CN 101336589 A CN101336589 A CN 101336589A
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- cutting
- cuttage
- plugged ear
- dendrobium candidum
- transplanting
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- 241001076416 Dendrobium tosaense Species 0.000 title abstract 2
- 238000012364 cultivation method Methods 0.000 title abstract 2
- 239000000463 material Substances 0.000 claims abstract description 22
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 238000005286 illumination Methods 0.000 claims abstract description 8
- 241000026010 Dendrobium candidum Species 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 5
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 5
- 235000011613 Pinus brutia Nutrition 0.000 claims description 5
- 241000018646 Pinus brutia Species 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 230000011218 segmentation Effects 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 239000012286 potassium permanganate Substances 0.000 abstract description 2
- 239000004745 nonwoven fabric Substances 0.000 abstract 1
- 238000010008 shearing Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000748029 Chamaechaenactis scaposa Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 241001523681 Dendrobium Species 0.000 description 1
- 241000594394 Hedyotis Species 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
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- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a cuttage cultivation method of dendrobium officinale, which comprises the following steps: preparing cutting materials: cutting the pseudobulb from the root of the parent body to be used as a cutting material; cutting materials are segmented: cutting the pseudobulb into cutting slips, wherein each cutting slip is provided with 2-3 nodes; shearing off the blade between the sections; soaking the cutting slips in a potassium permanganate solution and then airing; cutting and cutting: inserting the cutting slips into the prepared cutting medium, watering thoroughly once, and covering a layer of wet non-woven fabric; managing the cutting: the culture environment is in a semi-negative and humid state, the temperature is in the range of 5-35 ℃, and the illumination is in the range of 1500-; transplanting: 3-4 months after cuttage, cutting off new plants by using sterilized scissors, and transplanting the plants into a transplanting medium. The plant bred by the method can completely preserve the excellent quality of the parent body, does not have variation, has predictable culture results, reduces the risk of breeding and can shorten the breeding period.
Description
Technical field
The present invention relates to a kind of artificial culturing method of dendrobium candidum, especially the cottage breeding method of dendrobium candidum.
Background technology
Dendrobium candidum has another name called ribbed hedyotis herb (Dendrobim officinale Kimra et Migo), belongs to Angiospermae, unifacial leaf guiding principle, little specific item, the orchid family, Dendrobium, is a kind of famous and precious medicinal material.Because producing region medicinal herb grower's destructiveness is excavated, and makes wild dendrobium candidum resource endangered, country encourages scientific research institution and enterprises and institutions that dendrobium candidum is carried out artificial culture.Application number is that the Chinese invention patent " the artificial large-area cultivation of dendrobium candidum " of CN 200410084496.1 has been announced and a kind ofly utilized the seed of dendrobium candidum numerous by exclusive expansion, practices seedling, transplanting, collecting method, obtains the way of good society and economic benefit; Application number is that the Chinese invention patent " a kind of seedling-cultivating method of dendrobium candidum stem apex quick breeding by group culture " of CN200710066480.1 discloses and a kind ofly utilizes wild dendrobium candidum seedling to carry out the training approach of inducing culture, enrichment culture, differentiation culture, strong sprout and culture of rootage, shortens cultivation period, keeps the stabilization characteristics of genetics of wild dendrobium candidum.
As mentioned above, the breeding method of existing dendrobium candidum is to utilize the seed of dendrobium candidum or sprouting to cultivate mostly, and what promptly utilize is the sexual propagation of dendrobium candidum.As everyone knows, dendrobium candidum after having carried out sexual propagation, its hereditary feature possibly relatively parent change the quality of cultivation personnel after the seed that can't learn its cultivation before the cultivation or sprouting grow up to ripe plant.The cultivation personnel are in when cultivation, need emit some plant of turning out in the future can not adopted risk.
Summary of the invention
The object of the present invention is to provide a kind of vegetative propagation breeding method of dendrobium candidum.
The technical solution adopted in the present invention is: 1. prepare the cuttage material: select that quality is good, the dendrobium candidum plant parent of robust growth, no damage by disease and insect, with pseudobulb from the foundation of parent cut off, as the cuttage material;
2. cuttage material segmentation: pseudobulb is cut into chunks with the scraper of sterilizing---form plugged ear, 2-3 joint arranged on each plugged ear; The blade of internode is cut; Plugged ear after being to soak 5 minutes in 1 ‰ the liquor potassic permanganate, mass content is dried;
3. cuttage plugged ear: plugged ear is inserted in the ready cutting medium, water once permeable, the nonwoven that upper cover one deck is wet;
4. manage plugged ear: make culture environment be in half the moon, dampness, temperature is 5 ℃ of-35 ℃ of scopes, and illumination is in the 1500-5000lux scope;
5. transplant: after the cuttage 3-4 month, the root system of plugged ear grew to the 3-5 root, during every long 2-3cm, with the scissors of sterilizing new plant was sheared off, and transplanted to transplanting medium.
Another technical scheme of the present invention is: 1. prepare the cuttage material: select that quality is good, the dendrobium candidum plant parent of robust growth, no damage by disease and insect, with pseudobulb from the foundation of parent cut off, as the cuttage material;
2. cuttage material processed: the top first segment that removes the cuttage material; The blade of internode is cut; Plugged ear after being to soak 5 minutes in 1 ‰ the liquor potassic permanganate, mass content is dried;
3. cuttage plugged ear: plugged ear is inserted in the ready cutting medium, water once permeable, the nonwoven that upper cover one deck is wet;
4. manage plugged ear: make culture environment be in half the moon, dampness, temperature is 5 ℃ of-35 ℃ of scopes, and illumination is in the 1500-5000lux scope;
5. transplant: after the cuttage 3-4 month, the root system of plugged ear grew to the 3-5 root, during every long 2-3cm, with the scissors of sterilizing new plant was sheared off, and transplanted to transplanting medium.
More than in two technical schemes, the humidity of the cutting medium of step in 3. is preferably in the 60%-80% scope; The mixture of transplanting medium preferred careless carbon, fermentation pine squama and the fermentation wood chip of step in 5., mixed transplanting medium water content is 50%-70%, transplanting cultivation temperature is 5 ℃ of-35 ℃ of scopes.
The present invention proposes a kind of vegetative propagation breeding method of dendrobium candidum, the pseudobulb that utilizes dendrobium candidum is for cultivating object, the plant that breeds out can preserve the fine quality of parent completely, can not morph, the cultivation achievement of this kind cultural method can be predicted, reduce the risk of cultivating, and can shorten cultivation period.
Embodiment
Embodiment 1---the segmentation cuttage
Cutting time: annual feasible, Best Times is February-May.
The cuttage container: the wide * height of long * is the square position of 54cm*27cm*6cm.
Cutting medium: sphagna is soaked dehydration after 15 minutes, pulls diffusing, place mat in the square position in clear water, laying depth is 2.5cm to a 4.5cm scope, with mass content is that 1 ‰ carbendazim solution waters sterilization to cutting medium, and the humidity of guaranteeing cutting medium is in the 60%-80% scope.
Culture environment: plastic tunnel, canopy has the sunshade net outward, and the internal sunshade net is arranged in the canopy, adopts double-deck sunshade net to reduce illumination, intensity of illumination is controlled at the 1500-30000lus scope, need control light intensity during cuttage in low scope and along with light intensity is strengthened in the growth of dendrobium candidum gradually.
Prepare the cuttage material: select that quality is good, the dendrobium candidum plant parent of robust growth, no damage by disease and insect, with scissors pseudobulb is cut off from the foundation of dendrobium candidum, as the cuttage material (plant that grows fine usually,, pseudobulb has the 1-6 root, and every pseudobulb generally has 4-12 joint).
The segmentation of cuttage material: at first, with the alcohol disinfecting scraper of volume content 75%; Secondly, pseudobulb is cut into chunks with scraper---form plugged ear, guarantee to have on each plugged ear 2-3 joint,, the blade of internode is cut for preventing the moisture loss after the cuttage; Once more, be to soak 5 minutes in 1 ‰ the liquor potassic permanganate plugged ear in mass content, at the moon place that ventilates plugged ear is dried.
The cuttage plugged ear: plugged ear is inserted in the ready cutting medium, and spacing in the rows is in the 3cm-4.5cm scope, water once permeable, the nonwoven that upper cover one deck is wet.Before the cuttage,, also plugged ear can be immersed in the heteroauxin solution of 300ppm and soak 10 minutes, then plugged ear be taken out, dried its surperficial moisture a little, insert again in the ready cutting medium in order to induce cuttage rooting, germination.
The management plugged ear: after the plugged ear cuttage, environment is in half the moon, dampness in the canopy, and temperature is 5 ℃ of-35 ℃ of scopes, and illumination is in the 1500-5000lux scope; When slightly do on the cutting medium surface, promptly sprayed water; After one month, available nitrogen: phosphorus: the potassium mass ratio is that 20: 20: 20, mass concentration are that 0.33 ‰ balance fertilizer sprays; 1-2 is individual month after the plugged ear cuttage, has sprouting to grow at the position of joint, and 3-4 is individual month after the cuttage, and root system is formed at the sprouting bottom, can transplant.By the plugged ear that patented method of the present invention is cultivated, can take root and be used for more than 92% to transplant, 90% survival rate of comparing traditional sexual reproduction method is slightly high; The cultivation period of patent of the present invention is short, compares traditional 8-10 month cultivation period, obviously shortens cultivation period, and economic benefit is obvious.
Transplant: the plugged ear root system for the treatment of the dendrobium candidum seedling in the square position grows to the 3-5 root, during every long 2-3cm, can transplant; Transplanting medium is selected the mixture of careless carbon, fermentation pine squama and fermentation wood chip for use, transplanting medium before use should be through high-temperature sterilization, the necessary complete fermentation of pine squama and wood chip, mixed transplanting medium water content are about 60% (agglomerating to hold, as to unclamp the degree of promptly loosing and be as the criterion); Must new plant seedling be sheared off with the scissors of sterilizing during transplanting, because as mentioned above, include 2-3 joint on the plugged ear, between joint, sprout the new root of sprouting easily, after 3-4 month, several new plant seedlings can occur on the plugged ear, the plant these new sproutings during transplanting shears off, and the sterilization of scissors can be alcohol or potassium permanganate; Sphagna subsidiary on the root system is removed fully; Dendrobium candidum seedling growth preference temperature is 5 ℃ of-35 ℃ of scopes, and the cultivation temperature of shed should be lower than 35 ℃ in vegetative period, and should be controlled at more than 5 ℃ winter.
Embodiment 2---full stem cuttage
Compare embodiment 1, full stem cuttage is not carried out segmentation to the cuttage material, but adopt the way of full stem cuttage: the pseudobulb that will cut from parent, remove the first segment on the top that growing point is arranged, the blade of internode is cut, be to soak 5 minutes in 1 ‰ the liquor potassic permanganate with mass content, dry, lie against in the cutting medium in the ventilation.
Claims (6)
1, a kind of cottage breeding method of dendrobium candidum is characterized in that, comprises following steps:
1. prepare the cuttage material: select that quality is good, the dendrobium candidum plant parent of robust growth, no damage by disease and insect, with pseudobulb from the foundation of parent cut off, as the cuttage material;
2. cuttage material segmentation: pseudobulb is cut into chunks with the scraper of sterilizing---form plugged ear, 2-3 joint arranged on each plugged ear; The blade of internode is cut; Plugged ear after being to soak 5 minutes in 1 ‰ the liquor potassic permanganate, mass content is dried;
3. cuttage plugged ear: plugged ear is inserted in the ready cutting medium, water once permeable, the nonwoven that upper cover one deck is wet;
4. manage plugged ear: make culture environment be in half the moon, dampness, temperature is 5 ℃ of-35 ℃ of scopes, and illumination is in the 1500-5000lux scope;
5. transplant: after the cuttage 3-4 month, the root system of plugged ear grew to the 3-5 root, during every long 2-3cm, with the scissors of sterilizing new plant was sheared off, and transplanted to transplanting medium.
2, the cottage breeding method of a kind of dendrobium candidum according to claim 1 is characterized in that: the humidity of the cutting medium of step in 3. is in the 60%-80% scope.
3, the cottage breeding method of a kind of dendrobium candidum according to claim 1, it is characterized in that: the transplanting medium of step in 5. selected the mixture of careless carbon, fermentation pine squama and fermentation wood chip for use, mixed transplanting medium water content is 50%-70%, and transplanting cultivation temperature is 5 ℃ of-35 ℃ of scopes.
4, a kind of cottage breeding method of dendrobium candidum is characterized in that, comprises following steps:
1. prepare the cuttage material: select that quality is good, the dendrobium candidum plant parent of robust growth, no damage by disease and insect, with pseudobulb from the foundation of parent cut off, as the cuttage material;
2. cuttage material processed: the top first segment that removes the cuttage material; The blade of internode is cut; Plugged ear after being to soak 5 minutes in 1 ‰ the liquor potassic permanganate, mass content is dried;
3. cuttage plugged ear: plugged ear is inserted in the ready cutting medium, water once permeable, the nonwoven that upper cover one deck is wet;
4. manage plugged ear: make culture environment be in half the moon, dampness, temperature is 5 ℃ of-35 ℃ of scopes, and illumination is in the 1500-5000lux scope;
5. transplant: after the cuttage 3-4 month, the root system of plugged ear grew to the 3-5 root, during every long 2-3cm, with the scissors of sterilizing new plant was subtracted, and transplanted to transplanting medium.
5, the cottage breeding method of a kind of dendrobium candidum according to claim 4 is characterized in that: the humidity of the cutting medium of step in 3. is in the 60%-80% scope.
6, the cottage breeding method of a kind of dendrobium candidum according to claim 4, it is characterized in that: the transplanting medium of step in 5. selected the mixture of careless carbon, fermentation pine squama and fermentation wood chip for use, mixed transplanting medium water content is 50%-70%, and transplanting cultivation temperature is 5 ℃ of-35 ℃ of scopes.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008100217906A CN101336589A (en) | 2008-08-13 | 2008-08-13 | Cuttage cultivation method for dendrobium officinale |
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| CNA2008100217906A CN101336589A (en) | 2008-08-13 | 2008-08-13 | Cuttage cultivation method for dendrobium officinale |
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| CN101336589A true CN101336589A (en) | 2009-01-07 |
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Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695272B (en) * | 2009-10-26 | 2011-03-30 | 重庆市中药研究院 | Natural reproduction method for dendrobium |
| CN101999290A (en) * | 2010-09-14 | 2011-04-06 | 吴江市苗圃集团有限公司 | Cultivation method of dendrobium candidum |
| CN102598999A (en) * | 2012-03-30 | 2012-07-25 | 常熟市海虞茶叶有限公司 | Cultivation method of Dendrobium officinale |
| CN102668957A (en) * | 2012-06-01 | 2012-09-19 | 凌云县长生仙草生物科技开发有限公司 | Method for preparing dendrobium officinale culture substrate |
| CN102972267A (en) * | 2012-11-26 | 2013-03-20 | 广东省农业科学院作物研究所 | Cuttage seedling method for dendrobium candidum |
| CN103283429A (en) * | 2012-02-29 | 2013-09-11 | 宋维喜 | Method for planting ivy-arum |
| CN104641878A (en) * | 2015-01-05 | 2015-05-27 | 安徽省霍山县五峰山石斛开发有限公司 | Cuttage method of dendrobium huoshanense |
| CN104705175A (en) * | 2015-02-12 | 2015-06-17 | 贵州省亚热带作物研究所 | Dendrobium fimbriatum cutting seedling method |
| CN104838852A (en) * | 2015-05-12 | 2015-08-19 | 柳州市耕青科技有限公司 | Method for dendrobium officinale cuttage seedling cultivation |
| CN105010110A (en) * | 2015-07-03 | 2015-11-04 | 浙江仙人草保健品有限公司 | Method for planting dendrobium officinale by use of inner concave type seedbed |
| CN105075832A (en) * | 2015-09-22 | 2015-11-25 | 云南农业大学 | Normalized cultivation method for field cultivation of dendrobium officinale |
| CN105493839A (en) * | 2015-12-08 | 2016-04-20 | 安徽省霍山县农伯乐开发有限公司 | Cuttage seedling-raising method of dendrobium huoshanense |
| CN106106103A (en) * | 2016-07-11 | 2016-11-16 | 广西壮族自治区林业科学研究院 | Scribbling sketch Herba Dendrobii old stem cuttage breeding method |
| CN107223446A (en) * | 2017-07-24 | 2017-10-03 | 南京仙草堂生物科技有限公司 | A kind of Huangshi dry measure used in former times seedling cuttage rapid propagating method |
| CN107258517A (en) * | 2017-08-03 | 2017-10-20 | 南京仙草堂生物科技有限公司 | A kind of preparation method of yellow dendrobium tea |
| CN107593359A (en) * | 2017-09-26 | 2018-01-19 | 界首市致富家庭农场 | A kind of stem of noble dendrobium cutting propagation method of high-survival rate |
| CN108887162A (en) * | 2018-06-26 | 2018-11-27 | 赤水市信天中药产业开发有限公司 | A kind of HERBA DENDROBII cuttage breeding method |
| CN110402778A (en) * | 2019-07-15 | 2019-11-05 | 浙江省农业科学院 | Improve the Emulational culture method and facility of fresh type dendrobium candidum Clonal Cultivar yield |
| CN113348806A (en) * | 2021-07-01 | 2021-09-07 | 浙江省农业科学院 | Method for promoting lateral bud of pseudobulb of orchid to sprout in advance |
| CN114916328A (en) * | 2022-05-31 | 2022-08-19 | 河北鲜达农业科技有限公司 | Rooting method for dendrobium officinale |
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2008
- 2008-08-13 CN CNA2008100217906A patent/CN101336589A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695272B (en) * | 2009-10-26 | 2011-03-30 | 重庆市中药研究院 | Natural reproduction method for dendrobium |
| CN101999290A (en) * | 2010-09-14 | 2011-04-06 | 吴江市苗圃集团有限公司 | Cultivation method of dendrobium candidum |
| CN103283429A (en) * | 2012-02-29 | 2013-09-11 | 宋维喜 | Method for planting ivy-arum |
| CN102598999A (en) * | 2012-03-30 | 2012-07-25 | 常熟市海虞茶叶有限公司 | Cultivation method of Dendrobium officinale |
| CN102668957A (en) * | 2012-06-01 | 2012-09-19 | 凌云县长生仙草生物科技开发有限公司 | Method for preparing dendrobium officinale culture substrate |
| CN102972267A (en) * | 2012-11-26 | 2013-03-20 | 广东省农业科学院作物研究所 | Cuttage seedling method for dendrobium candidum |
| CN104641878A (en) * | 2015-01-05 | 2015-05-27 | 安徽省霍山县五峰山石斛开发有限公司 | Cuttage method of dendrobium huoshanense |
| CN104705175A (en) * | 2015-02-12 | 2015-06-17 | 贵州省亚热带作物研究所 | Dendrobium fimbriatum cutting seedling method |
| CN104838852A (en) * | 2015-05-12 | 2015-08-19 | 柳州市耕青科技有限公司 | Method for dendrobium officinale cuttage seedling cultivation |
| CN105010110A (en) * | 2015-07-03 | 2015-11-04 | 浙江仙人草保健品有限公司 | Method for planting dendrobium officinale by use of inner concave type seedbed |
| CN105075832A (en) * | 2015-09-22 | 2015-11-25 | 云南农业大学 | Normalized cultivation method for field cultivation of dendrobium officinale |
| CN105493839A (en) * | 2015-12-08 | 2016-04-20 | 安徽省霍山县农伯乐开发有限公司 | Cuttage seedling-raising method of dendrobium huoshanense |
| CN106106103A (en) * | 2016-07-11 | 2016-11-16 | 广西壮族自治区林业科学研究院 | Scribbling sketch Herba Dendrobii old stem cuttage breeding method |
| CN106106103B (en) * | 2016-07-11 | 2019-07-16 | 广西壮族自治区林业科学研究院 | Scribbling sketch dendrobium nobile old stem cuttage breeding method |
| CN107223446A (en) * | 2017-07-24 | 2017-10-03 | 南京仙草堂生物科技有限公司 | A kind of Huangshi dry measure used in former times seedling cuttage rapid propagating method |
| CN107258517A (en) * | 2017-08-03 | 2017-10-20 | 南京仙草堂生物科技有限公司 | A kind of preparation method of yellow dendrobium tea |
| CN107593359A (en) * | 2017-09-26 | 2018-01-19 | 界首市致富家庭农场 | A kind of stem of noble dendrobium cutting propagation method of high-survival rate |
| CN108887162A (en) * | 2018-06-26 | 2018-11-27 | 赤水市信天中药产业开发有限公司 | A kind of HERBA DENDROBII cuttage breeding method |
| CN110402778A (en) * | 2019-07-15 | 2019-11-05 | 浙江省农业科学院 | Improve the Emulational culture method and facility of fresh type dendrobium candidum Clonal Cultivar yield |
| CN113348806A (en) * | 2021-07-01 | 2021-09-07 | 浙江省农业科学院 | Method for promoting lateral bud of pseudobulb of orchid to sprout in advance |
| CN114916328A (en) * | 2022-05-31 | 2022-08-19 | 河北鲜达农业科技有限公司 | Rooting method for dendrobium officinale |
| CN114916328B (en) * | 2022-05-31 | 2023-12-01 | 河北鲜达农业科技有限公司 | Dendrobium officinale rooting method |
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