CN104705175A - Dendrobium fimbriatum cutting seedling method - Google Patents

Dendrobium fimbriatum cutting seedling method Download PDF

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Publication number
CN104705175A
CN104705175A CN201510074549.XA CN201510074549A CN104705175A CN 104705175 A CN104705175 A CN 104705175A CN 201510074549 A CN201510074549 A CN 201510074549A CN 104705175 A CN104705175 A CN 104705175A
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CN
China
Prior art keywords
cuttage
cutting
seedling
branch
dendrobium
Prior art date
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CN201510074549.XA
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Chinese (zh)
Inventor
敖茂宏
宋智琴
吴明开
罗晓青
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贵州省亚热带作物研究所
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Application filed by 贵州省亚热带作物研究所 filed Critical 贵州省亚热带作物研究所
Priority to CN201510074549.XA priority Critical patent/CN104705175A/en
Publication of CN104705175A publication Critical patent/CN104705175A/en

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust

Abstract

The invention discloses a dendrobium fimbriatum cutting seedling method which comprises the steps that cutting branch selecting is carried out, wherein triennial dendrobe branches are selected, and branch middle portions or top portions are taken; shoot cutting is carried out, wherein cutting ends of the dendrobium fimbriatum branches are subjected to oblique cutting by the angle of 30-60 degrees, then the cutting ends are soaked in an ABT rooting powder solution, and soaking time is 6 h; cutting propagation is carried out, wherein processed cutting branches are vertically inserted into a seedling culturing matrix where humic pine bark is paved; and seedling culturing management is carried out, a seedbed is subjected to watering every 5 days, temperature is controlled to be about 25 DEG C, humidity is controlled at about 60%, and when 3-5 roots grow at the cutting ends of the dendrobe cutting branches and buds start to grow from middle internodes, seedling transplantation can be carried out. The cutting branches are the middle portions or the upper portions of triennial dendrobe stem branches, material sources are wide, cost is low, local materials can be used, meanwhile, the selected seedling culturing matrix is the humic pine bark, the source is wide, cost is low, and seedling culturing effect is obvious.

Description

A kind of Dendrobium fimbriatum Hook cuttage and seedling culture method
Technical field
The present invention relates to a kind of Dendrobium fimbriatum Hook cuttage and seedling culture method, belong to orchid seedling transplanting technique field.
Background technology
Dendrobium fimbriatum Hook (Dendrobium fimbriatum Hook.), have another name called stem of Eyeshaped Dendrobium, be the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, be China's tradition rare traditional Chinese medicine, have and nourish lung stomach, effect of clearing heat and promoting fluid.The stem of noble dendrobium is traditional, genuine rare Chinese medicine, it is again the important source material of producing Chinese medicine now, Dendrobium fimbriatum Hook China be mainly distributed in south Guangxi to the northwestward, South of Guizhou to the west and south, southeastern Yunnan is to tropical and subtropical region such as the west and south, in the last few years due to excessively excavate and self growth, reproductive characteristic and the particular/special requirement to growing environment, wild resource is on the verge of exhaustion.
At present, in the artificial planting production process of Dendrobium fimbriatum Hook, breeding based on tissue cultures of Dendrobium fimbriatum Hook seedling, also exists the commercialization that the series of problems such as toxigenic capacity is high, cultivation operating condition harshness (needing aseptic), complex operation, medium are seriously polluted, cultivation cycle is long, seedling cost to sell is high hamper Dendrobium fimbriatum Hook seedling.For this reason, the success of Dendrobium fimbriatum Hook artificial seedling cutting propagation technology, to expansion stem of noble dendrobium provenance, develops artificial cultivation and protects this germ plasm resource in imminent danger to have great significance.
Summary of the invention
The object of the invention is in existing Dendrobium fimbriatum Hook seedling growing process, seedling breeding cost is high, complex operation, and a kind of Dendrobium fimbriatum Hook cuttage and seedling culture method provided, the method is practical, easy and simple to handle, with low cost, with strong points, and industrialization is effective, cuttage seeding planting percent is high, emerge neat, seedling grows fine, without damage by disease and insect, resistance, can provide high quality seedling aborning.
Technical scheme of the present invention: a kind of Dendrobium fimbriatum Hook cuttage and seedling culture method, comprises the following steps:
Step one, cuttage branch are selected: choose triennial stem of noble dendrobium branch, get in the middle part of its branch or top;
Step 2, cuttings process: the cuttage end of Dendrobium fimbriatum Hook branch is 30 ~ 60 degree and carries out bevel, make the length of cuttage branch be 3 ~ 8cm, be then soaked in ABT rooting powder solution by cuttage end, soak time is 6h;
Step 3, cottage propagation: the cuttage branch handled well is vertically inserted in the seedling medium completing humic pine bark, the cuttage degree of depth is exposed matrix 1 ~ 3cm with branch and is advisable, and seedling medium temperature controls, at about 25 DEG C, to spill permeable simultaneously;
Step 4, seedling management: a water was spilt every 5 days in seedbed, temperature controls at about 25 DEG C, and humid control, about 60%, is treated that stem of noble dendrobium cuttage branch cutting end starts long root 3 ~ 5, and when internode place, middle part starts to emerge sprouting, can be carried out seedling raising and transplanting.
The concentration of aforesaid ABT rooting powder solution is 100mg/L, and temperature is 35 DEG C.
Aforesaid humic pine bark adopts carbendazim to disinfect, and is ground into the fritter that diameter is 1 ~ 2cm, and be then laid in as seedling medium in seedbed using the pine bark after pulverizing, laying depth is 15 ~ 20cm.
Owing to adopting technique scheme, the invention has the advantages that: the present invention's cuttage branch used is in the middle part of 3 years raw stem of Dendrobium branches or top, its material source is extensive, with low cost, can gather materials on the spot, the seedling medium simultaneously selected adopts humic pine bark, wide material sources, with low cost, nursery effect is remarkable.Cuttage and seedling culture method of the present invention is practical, with low cost, with strong points, and nursery effect is good, can promote aborning.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearly, below in conjunction with embodiment, the present invention is described in further detail.
Embodiment
(1) cuttage branch is selected:
Choose triennial stem of noble dendrobium branch, get in the middle part of its branch or top, the necessary robust growth of cuttage branch, anosis branch;
(2) cuttings process:
The cuttage end of Dendrobium fimbriatum Hook branch is 30 ~ 60 degree and carries out bevel, make the length of cuttage branch be 3 ~ 8cm, be then soaked in ABT rooting powder solution by cuttage end, soak time is 6h.The concentration of described ABT rooting powder solution is 100mg/L, and temperature is 35 DEG C.
(3) selection of seedling medium:
Seedling medium adopts the pine bark of humic, dries after humic pine bark adopts carbendazim sterilization post processing, and then with cracker, to be ground into diameter be that the fritter of 1 ~ 2cm is for subsequent use is laid in seedbed by the pine bark after pulverizing, and thickness is 20cm.
(4) cottage propagation:
Vertically insert in the seedling medium completing humic pine bark by the cuttage branch handled well, the cuttage degree of depth is exposed matrix 1 ~ 3cm with branch and is advisable, and seedling medium temperature controls, at about 25 DEG C, to spill permeable simultaneously.
(5) later stage seedling management method:
Seedling medium spilt a water every 5 days, temperature controls at about 25 DEG C, humid control is about 60%, carry out observed and recorded, the dead branch that goes mouldy as found removes in time, after 20 days, treats that stem of noble dendrobium cuttage branch cutting end starts long root 3 ~ 5, and internode place, middle part is when starting to emerge sprouting, seedling raising and transplanting can be carried out.

Claims (3)

1. a Dendrobium fimbriatum Hook cuttage and seedling culture method, is characterized in that comprising the following steps:
Step one, cuttage branch are selected: choose triennial stem of noble dendrobium branch, get in the middle part of its branch or top;
Step 2, cuttings process: the cuttage end of Dendrobium fimbriatum Hook branch is 30 ~ 60 degree and carries out bevel, make the length of cuttage branch be 3 ~ 8cm, be then soaked in ABT rooting powder solution by cuttage end, soak time is 6h;
Step 3, cottage propagation: the cuttage branch handled well is vertically inserted in the seedling medium completing humic pine bark, the cuttage degree of depth is exposed matrix 1 ~ 3cm with branch and is advisable, and seedling medium temperature controls, at about 25 DEG C, to spill permeable simultaneously;
Step 4, seedling management: a water was spilt every 5 days in seedbed, temperature controls at about 25 DEG C, and humid control, about 60%, is treated that stem of noble dendrobium cuttage branch cutting end starts long root 3 ~ 5, and when internode place, middle part starts to emerge sprouting, can be carried out seedling raising and transplanting.
2. Dendrobium fimbriatum Hook cuttage and seedling culture method according to claim 1, is characterized in that: the concentration of described ABT rooting powder solution is 100mg/L, and temperature is 35 DEG C.
3. Dendrobium fimbriatum Hook cuttage and seedling culture method according to claim 1, it is characterized in that: described humic pine bark adopts carbendazim to disinfect, and be ground into the fritter that diameter is 1 ~ 2cm, then be laid in as seedling medium in seedbed using the pine bark after pulverizing, laying depth is 15 ~ 20cm.
CN201510074549.XA 2015-02-12 2015-02-12 Dendrobium fimbriatum cutting seedling method CN104705175A (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106358832A (en) * 2016-09-04 2017-02-01 吴玲 Cutting seedling raising method of dendrobium

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CN101156551A (en) * 2006-10-11 2008-04-09 中国林业科学研究院林业研究所 Dendrobium nobile stem segment cuttage method
CN101336589A (en) * 2008-08-13 2009-01-07 吴江市苗圃集团有限公司 Cottage breeding method of dendrobium officinale
CN102138420A (en) * 2011-02-22 2011-08-03 贵州省农作物品种资源研究所 Method for cultivating dendrobium fimbriatum
KR20120068242A (en) * 2010-12-17 2012-06-27 논산시(관리부서:논산시농업기술센터) Aeroponic culture system for setting plants production of strawberry through runner-cutting
CN103548655A (en) * 2013-11-14 2014-02-05 贵州省农作物品种资源研究所 Method for directly planting dendrobium fimbriatum tissue culture seedlings in field
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CN101156551A (en) * 2006-10-11 2008-04-09 中国林业科学研究院林业研究所 Dendrobium nobile stem segment cuttage method
CN101336589A (en) * 2008-08-13 2009-01-07 吴江市苗圃集团有限公司 Cottage breeding method of dendrobium officinale
KR20120068242A (en) * 2010-12-17 2012-06-27 논산시(관리부서:논산시농업기술센터) Aeroponic culture system for setting plants production of strawberry through runner-cutting
CN102138420A (en) * 2011-02-22 2011-08-03 贵州省农作物品种资源研究所 Method for cultivating dendrobium fimbriatum
CN103548655A (en) * 2013-11-14 2014-02-05 贵州省农作物品种资源研究所 Method for directly planting dendrobium fimbriatum tissue culture seedlings in field
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106358832A (en) * 2016-09-04 2017-02-01 吴玲 Cutting seedling raising method of dendrobium

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