CN108739321A - A kind of wild Dendrodium propagation method - Google Patents
A kind of wild Dendrodium propagation method Download PDFInfo
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- CN108739321A CN108739321A CN201810519586.0A CN201810519586A CN108739321A CN 108739321 A CN108739321 A CN 108739321A CN 201810519586 A CN201810519586 A CN 201810519586A CN 108739321 A CN108739321 A CN 108739321A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
Abstract
The present invention provides a kind of wild Dendrodium propagation method, and selection is laid with the fermentation degreasing tree skin layer by sterilization convenient for the seedling bed of draining on seedling bed, first moisturizing layer of the setting by sterilization on the fermentation degreasing tree skin layer；Wild Dendrodium erect stern is cut into section, dries in the shade to wound and dries；Wild Dendrodium stem section after drying in the shade is laid on the first moisturizing layer, then the second moisturizing layer is covered above wild stem of Dendrobium section, starts to seal bud；After sealing bud 20-30 days, axillary bud is selected directly to transplant.Modes of reproduction using the present invention, it is at low cost, it is easy to operate.Modes of reproduction using the present invention also continues to breed using the plant of root necrosis or stem's lesion.Compared with being grown with tissue-culturing rapid propagation, the present invention seal bud breeding axillary bud sprouting seedling it is sturdy, can directly transplant, be not necessarily to hardening, method is simple, at low cost.Survival rate after transplanting is high, can be effectively protected wild Dendrodium resource in imminent danger, expands wild Dendrodium resource population quantity.
Description
Technical field
The invention belongs to field of plant reproduction, are related to stem of noble dendrobium reproduction technique, and in particular to the propagation method of wild Dendrodium.
Background technology
Dendrobium resource is I grade of China protection plant, belongs to endangered plants resource, and resource is less, and natural propagation rate is low,
Virosis easily occurs in domesticating and cultivating, causes plant dead, resource material is caused to be lost in.There are 76 kinds of Dendrobium Plants in China, at present
Only a small number of dendrobium species pass through sterile tissue culture method expanding propagation, such as dendrobium candidum, nobile-type dendrobium, Dendrobium Moniliforme etc..
In realizing process of the present invention, inventor has found that at least there are the following problems in the prior art：
1, the tissue culture propagation stem of noble dendrobium needs corresponding facility and aseptic strain operation, although output of seedling is larger, seedling is weaker, emergence
Directly disease easily occurs for transplanting afterwards, causes the death rate higher, survival rate is relatively low；
2, hardening technical optimization nurturing staff is also needed before being colonized after emerging by the bottle seedling of tissue culture expanding propagation, the period is long, of high cost.
Invention content
In consideration of it, present invention aims at providing, a kind of survival rate is high, the breeding cycle is short, wild Dendrodium at low cost breeding
Method.
Inventor passes through long-term exploration and trial and multiple experiment and effort, continuous reform and innovation, to solve
The above technical problem, technical solution provided by the invention are to provide a kind of wild Dendrodium propagation method, include the following steps：
Step a）Whole bed
Selection is laid with the fermentation degreasing tree skin layer by sterilization convenient for the seedling bed of draining on seedling bed, de- in the fermentation
First moisturizing layer of the setting by sterilization on fat tree skin layer；
Step b）Cut stem
Wild Dendrodium erect stern is cut into section, dries in the shade to wound and dries；
Step c）Seal bud
Wild Dendrodium stem section after drying in the shade is laid on the first moisturizing layer, then covers the second moisturizing above wild stem of Dendrobium section
Layer, starts to seal bud；
Step d）Transplanting
After sealing bud 20-30 days, axillary bud is selected directly to transplant.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the step a）In, ferment degreasing bark block
Diameter is 8-18mm.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the first moisturizing layer be solid carbon dioxide tongue or
Dry moss is laid with, and the second moisturizing layer is that solid carbon dioxide tongue or dry moss are laid with.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the first moisturizing layer thickness is 3cm,
The second moisturizing layer thickness is 2cm.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the step b）In, healthy plant,
Wild Dendrodium erect stern is cut into the section of 10-15cm long, the small varieties of resources of plant type can be cut into 5cm long or directly using upright
Stem dries in the shade 1-2 days.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the step b）In, root necrosis or
The plant of stem's lesion, first wipes out diseased region, then by stem soaking sterilization, dries in the shade after sterilization 2-3 days.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the step c）In, sealing bud temperature is
28 DEG C -35 DEG C, it is 80%-90% to seal bud humidity.It is any temperature in 28 DEG C to 35 DEG C to seal bud temperature, including but not limited to 28
DEG C, 29 DEG C, 30 DEG C, 31 DEG C, 32 DEG C, 33 DEG C, 34 DEG C or 35 DEG C, preferably seal bud temperature be 30 DEG C.It is 80%-90% to seal bud humidity,
Refer to the moisture in the section, including but not limited to 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,
90%, it is 85% preferably to seal bud humidity.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the step c）In, it is sprayed after sealing bud 5 days
Apply root-growing agent.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the sterilization is killed using fungicide immersion
Bacterium.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the wild Dendrodium includes golden hairpin stone
Dry measure used in former times, the big luxuriant sheath stem of noble dendrobium, Dendrobium crepidatum lindl et paxt., swollen arthrolith dry measure used in former times, Dendrobium loddigesii, dendrobium candidum, dendrobium moniliformeSweet and Herba Dendrobii.
According to a better embodiment of wild Dendrodium propagation method of the present invention, the wild Dendrodium be HERBA DENDROBII,
The big bud sheath stem of noble dendrobium or Dendrobium crepidatum lindl et paxt..
Compared with prior art, a technical solution in above-mentioned technical proposal has the following advantages that：
A) modes of reproduction using the present invention, it is at low cost, it is easy to operate.
B) modes of reproduction using the present invention also continues to breed using the plant of root necrosis or stem's lesion.
C) compared with being grown with tissue-culturing rapid propagation, the present invention seal bud breeding axillary bud sprouting seedling it is sturdy, can directly transplant, be not necessarily to hardening,
Method is simple, at low cost.Survival rate after transplanting is high, can be effectively protected wild Dendrodium resource in imminent danger, expands wild Dendrodium money
Source population quantity.
D) in one embodiment of propagation method of the present invention, stem of noble dendrobium stem section, which is dried in the shade to wound, to be dried, and is conducive to avoid wound
Introduced disease.Higher due to sealing bud breeding environment temperature, humidity is larger, so needing to dry in the shade that wound is made to dry, avoids wound sense
It catches an illness evil.
E) in one embodiment of propagation method of the present invention, the plant of root necrosis or stem's lesion needs to dry in the shade after sterilization
2-3 days, it can effectively reduce forth infection disease.
F) in one embodiment of propagation method of the present invention, select solid carbon dioxide tongue as moisturizing material, solid carbon dioxide tongue has preferably
Moisture retention, moisture preserving time is longer, and gas permeability is preferable, and stem of noble dendrobium stem section can be maintained to be in the state of high humidity, promotes axillary bud differentiation.
G) moisturizing layer thickness is limited in one embodiment of propagation method of the present invention, thickness is too low, is easy to lead
Cause water supply in media inadequate, Yi Gan is unfavorable for axillary bud catalysis；The excessively high then humidity of moisturizing layer thickness is too high, is easy to cause disease.
H) reach 90% or more with HERBA DENDROBII, swollen arthrolith dry measure used in former times, the big luxuriant sheath stem of noble dendrobium, the Dendrobium crepidatum lindl et paxt. germination rate of sealing bud breeding,
Dendrobium loddigesii, dendrobium candidum, dendrobium moniliformeSweet, Herba Dendrobii germination rate reach 85% or more.
I) reach 90% or more with the HERBA DENDROBII, swollen arthrolith dry measure used in former times, the bud sheath stem of noble dendrobium, Dendrobium crepidatum lindl et paxt. survival rate greatly of sealing bud breeding；
Dendrobium loddigesii, dendrobium candidum, dendrobium moniliformeSweet, Herba Dendrobii survival rate reach 88% or more.
Specific implementation mode
It is illustrated with reference to specific embodiment.
To keep the purpose, technical scheme and advantage of embodiment of the present invention clearer, implement below in conjunction with the present invention
The technical solution in embodiment of the present invention is clearly and completely described in mode, it is clear that described embodiment is
Some embodiments of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this field is common
The every other embodiment that technical staff is obtained without creative efforts, belongs to protection of the present invention
Range.Therefore, the model of claimed invention is not intended to limit to the detailed description of embodiments of the present invention below
It encloses, but is merely representative of the selected embodiment of the present invention.
The shelf that height from the ground is 50cm is set in greenhouse booth, is convenient for draining and daily management, by flat plate
It is horizontally arranged on the top of the shelf, builds seedling bed.Leakage hole is provided on flat plate, convenient for draining extra water.
Fermentation degreasing bark is impregnated 3 hours with 50% carbendazol wettable powder, 500 times of liquid and is sterilized, is put down after sterilization
It is layered on flat plate.Fermentation degreasing bark block diameter is 8-18mm.Untreated bark contains grease and harmful levels of pathogens etc., hair
Ferment can remove harmful levels of pathogens and worm's ovum, and degreasing mainly uses high temperature and pressure boiling, fails to get rid of not in removal fermentation
Conducive to the lipid material of plant growth, while enhancing the water-retaining property and keeping fertility of bark.Bark after degreasing fermentation is not perishable,
It is not acidified, gas permeability is also preferable, and the bark matrix of non-degreasing fermentation process easily leads to the diseases such as the soft corruption of plant.
Solid carbon dioxide tongue or dry moss 50% carbendazol wettable powder, 500 times of liquid are impregnated 1 hour, hair is laid in after sterilization
On ferment degreasing bark, as the first moisturizing layer.Solid carbon dioxide tongue or dry moss refer to the material used in moisturizing layer, during not sealing bud
Time of day.During sealing bud, needs to spray water to solid carbon dioxide tongue or dry moss, keep the humidity of solid carbon dioxide tongue or dry moss.This reality
It applies in example, using solid carbon dioxide tongue as the first moisturizing layer material.Solid carbon dioxide tongue has preferable moisture retention, and moisture preserving time is longer, gas permeability
Preferably, the state of erect stern high humidity can be maintained, axillary bud differentiation is promoted.The solid carbon dioxide tongue or dry moss tiled on fermentation degreasing bark
Thickness for 3cm to be advisable.Solid carbon dioxide tongue or dry moss depth are then easy to cause that moisturizing material moisture is inadequate, and Yi Gan is unfavorable less than 3cm
It is catalyzed in axillary bud；Thickness can be very high higher than humidity in 3cm then moisturizing material, is easy to cause disease.Solid carbon dioxide tongue refer to by
The sphagna of drying process has lost physiological function, in embodiments of the present invention as just moisturizing material.Dry moss refers to
By the moss of drying process, physiological function is lost, in embodiments of the present invention as just moisturizing material.
Wild Dendrodium erect stern is cut into the section of a length of 10-15cm, cutting rear erect stern need to be disease-free.The small resource product of plant type
Kind can be cut into 5cm or so or directly use erect stern.The upright stem section of wild Dendrodium sheared is dried in the shade 1-2 days, is caused at shearing
Wound is dried.Higher due to sealing bud breeding environment temperature, humidity is larger, so needing to dry in the shade that wound is made to dry, avoids wound sense
It catches an illness evil.
If material includes the plant of root necrosis or stem's lesion, the stem section after diseased region is cut is soaked in 70%
It sterilizes, dries in the shade after sterilization 2-3 days in 500 times of liquid of thiophanate-methyl pulvis, to reduce forth infection disease.
To dry in the shade treated, wild Dendrodium stem section is laid on the first moisturizing layer, and 1- is covered again above wild Dendrodium stem section
Second moisturizing layer of 2cm thickness.Second moisturizing layer is identical as the material of the first moisturizing layer and material sterilization mode.It is sprayed to moisturizing layer
Water keeps moisturizing layer moistening, starts to seal bud.It is 28 DEG C -35 DEG C to seal bud temperature, and it is 80%-90% to seal bud humidity.Seal bud temperature be
Any temperature in 28 DEG C to 35 DEG C, including but not limited to 28 DEG C, 29 DEG C, 30 DEG C, 31 DEG C, 32 DEG C, 33 DEG C, 34 DEG C or 35 DEG C,
It is 30 DEG C that bud temperature is sealed in the present embodiment.It is 80%-90% to seal bud humidity, refers to the moisture in the section, including but unlimited
It is 85% that bud humidity is sealed in 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, the present embodiment.This implementation
Example is that bud is sealed in greenhouse booth, and there is good facility to ensure for the temperature and humidity control of matrix.
It seals and sprays root-growing agent after bud starts 5 days, root-growing agent main component includes methyl α-naphthyl acetate, indolebutyric acid sodium, trace element
Deng.Root-growing agent spraying method is to be watered 400ml per 1g root-growing agents.Root-growing agent belongs to commercial product, can be in Chinese pesticide Information Network
And dependent merchandise is inquired with principal component on related web site.
Seal bud after 20-30 days i.e. can be selected axillary bud directly transplant, be not required to hardening.
Modes of reproduction using the present invention, it is at low cost, it is easy to operate, also using root necrosis or the plant of stem's lesion
Continue to breed.Compared with being grown with tissue-culturing rapid propagation, the present invention seals that bud modes of reproduction cost is lower, and method is simple, is not necessarily to hardening, and axillary bud is sprouted
It is sturdy to send out seedling, can directly transplant, the survival rate after transplanting is high, can be effectively protected wild Dendrodium resource in imminent danger, expand wild stone
Dry measure used in former times resource population quantity.
For the technique effect further illustrated the present invention, illustrated below in conjunction with contrast test data.
Experiment one：The transplanting survival rate of different hardening off method is tested.
Using HERBA DENDROBII tissue-cultured seedling as test material, four kinds of hardening treatments of design are as a contrast.In handling below, processing one
It is control group to processing four, processing five is experimental group.
Processing one：Transplanting goes out to greenhouse, envelope bottle hardening 15 days for first 17 days, then opens hardening 2 days.
Processing two：Greenhouse directly is moved to from tissue culture room before transplanting, without greenhouse hardening, is directly transplanted.
Processing three：It is grown 40 days in tissue culture room after inoculation, moves to bottle outlet transplanting after 30 days under the conditions of greenhouse.
Processing four：It is grown 30 days in tissue culture room after inoculation, moves to bottle outlet transplanting after 40 days under the conditions of greenhouse.
Processing five：Using HERBA DENDROBII as test material, the axillary bud that bud breeding is sealed using the embodiment of the present invention is directly transplanted.
Pilot survey project and investigation Recording criteria：
A survival rates：
Observation in every 5 days is primary, and continuous observation 8 times every time notes down the survival seedling number of full cell, all without thoroughly dead
Plant be accordingly to be regarded as survival seedling, but basal part of stem is withered or dies of illness but seedling that top is not yet dead will regard as dead seedling.
B characters are investigated：
It investigates within the 5th day after transplanting, per the stem thickness of cell 10 plants of plant of random measurement, is averaged as district observation value.Experiment
Data are shown in Table 1.
As can be seen from Table 1, the hardening experiment survival rate after tissue culture is low, and the best processing one of survival rate also only has 46%.Place
Two not hardening of reason, is directly transplanted from tissue culture room bottle outlet, and test tube seedling is difficult to adapt to the variation of environment, the diseases such as corruption of easily feeling like jelly, also pole
Easily dehydration withered death.Processing three and processing four are seriously polluted due under the extensive environment of greenhouse, and in bottle cap sealing
In the case of, it easily heats up in bottle, causes bottle seedling to be dehydrated withered or tip segment and wither, greatly reduce resistance and survival rate.Processing
Survival rate highest after the directly transplanting of five axillary buds using the present invention for sealing bud breeding.
Table 1：Different hardening off method and the transplanting survival rate for sealing bud breeding
Different hardening off method and the stem thickness data statistics for sealing bud breeding are shown in Table 2.
As known from Table 2, it is 2. 13cm using the plant stem thickness highest of processing one in different Trained Transition Methods of Chinese Dwarf.It is numerous using bud is sealed
The axillary bud sprouting seedling stem thickness 3.43cm grown, it is obviously more sturdy than tissue-cultured seedling to seal the axillary bud sprouting seedling plant that bud is bred.
Table 2：Different hardening off method and the stem thickness for sealing bud breeding
Experiment two：Different cultivars seals the germination rate of bud breeding.
Using the erect stern of 8 kinds of different stems of noble dendrobium as test material, 1 bud propagation method is sealed through this embodiment, count rudiment
Rate, statistical result are shown in Table 3.
By 3 statistical result of table it is found that sealing bud breeding using erect stern, breeding amount is more, and every section of erect stern can occur 2-5
Axillary bud, axillary bud germination rate are all higher.
Wherein, with HERBA DENDROBII, swollen arthrolith dry measure used in former times, the big luxuriant sheath stem of noble dendrobium, the Dendrobium crepidatum lindl et paxt. germination rate of sealing bud breeding reach 90% with
On.Dendrobium loddigesii, dendrobium candidum, dendrobium moniliformeSweet, Herba Dendrobii germination rate reach 85% or more.
Table 3：Germination rate
| Kind | Erect stern item number | Sprout the erect stern item number of axillary bud | Germination rate（%） |
| HERBA DENDROBII | 72 | 70 | 97% |
| Swollen arthrolith dry measure used in former times | 60 | 55 | 92% |
| The big bud sheath stem of noble dendrobium | 62 | 58 | 94% |
| Dendrobium crepidatum lindl et paxt. | 42 | 40 | 95% |
| Dendrobium loddigesii | 56 | 48 | 86% |
| Dendrobium candidum | 38 | 33 | 87% |
| Dendrobium moniliformeSweet | 42 | 36 | 86% |
| Herba Dendrobii | 68 | 60 | 88% |
Experiment three：Different cultivars seals the survival rate of bud breeding.
Using the erect stern of 8 kinds of different stems of noble dendrobium as test material, bud propagation method is sealed through this embodiment, the 30th after transplanting
It notes down survival seedling number, all to be accordingly to be regarded as survival seedling without thorough dead plant, but basal part of stem is withered or dies of illness
But the not yet dead seedling in top will regard as dead seedling, and data statistics the results are shown in Table 4.
It can be seen from Table 4 that being survived with HERBA DENDROBII, swollen arthrolith dry measure used in former times, the big luxuriant sheath stem of noble dendrobium, the Dendrobium crepidatum lindl et paxt. for sealing bud breeding
Rate reaches 90% or more；Dendrobium loddigesii, dendrobium candidum, dendrobium moniliformeSweet, Herba Dendrobii survival rate reach 88% or more.
Table 4：Survival rate
| Kind | Axillary bud sprouting total amount | Survival seedling number after transplanting | Survival rate |
| HERBA DENDROBII | 186 | 180 | 97% |
| Swollen arthrolith dry measure used in former times | 111 | 104 | 94% |
| The big bud sheath stem of noble dendrobium | 120 | 112 | 93% |
| Dendrobium crepidatum lindl et paxt. | 88 | 80 | 91% |
| Dendrobium loddigesii | 138 | 122 | 88% |
| Dendrobium candidum | 68 | 60 | 88% |
| Dendrobium moniliformeSweet | 78 | 70 | 89% |
| Herba Dendrobii | 196 | 175 | 89% |
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be subject to claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
Protection scope of the present invention is also should be regarded as into retouching.
Claims (10)
1. a kind of wild Dendrodium propagation method, which is characterized in that include the following steps：
Step a）Whole bed
Selection is laid with the fermentation degreasing tree skin layer by sterilization convenient for the seedling bed of draining on seedling bed, de- in the fermentation
First moisturizing layer of the setting by sterilization on fat tree skin layer；
Step b）Cut stem
Wild Dendrodium erect stern is cut into section, dries in the shade to wound and dries；
Step c）Seal bud
Wild Dendrodium stem section after drying in the shade is laid on the first moisturizing layer, then covers the second moisturizing above wild stem of Dendrobium section
Layer, starts to seal bud；
Step d）Transplanting
After sealing bud 20-30 days, axillary bud is selected directly to transplant.
2. wild Dendrodium propagation method according to claim 1, which is characterized in that the step a）In, degreasing tree of fermenting
Skin bit diameter is 8-18mm.
3. wild Dendrodium propagation method according to claim 1, which is characterized in that the first moisturizing layer be solid carbon dioxide tongue or
Dry moss is laid with, and the second moisturizing layer is that solid carbon dioxide tongue or dry moss are laid with.
4. wild Dendrodium propagation method according to claim 1 or 3, which is characterized in that the first moisturizing layer thickness is
3cm, the second moisturizing layer thickness are 2cm.
5. wild Dendrodium propagation method according to claim 1, which is characterized in that the step b）In, healthy plant,
Wild Dendrodium erect stern is cut into the section of 10-15cm long, the small varieties of resources of plant type can be cut into 5cm long or directly using upright
Stem dries in the shade 1-2 days；The plant of root necrosis or stem's lesion, first wipes out diseased region, then by stem soaking sterilization, after sterilization
It dries in the shade 2-3 days.
6. wild Dendrodium propagation method according to claim 1, which is characterized in that the step c）In, sealing bud temperature is
28 DEG C -35 DEG C, it is 80%-90% to seal bud humidity.
7. wild Dendrodium propagation method according to claim 1 or 6, which is characterized in that the step c）In, seal bud 5 days
After spray root-growing agent.
8. wild Dendrodium propagation method according to claim 1, which is characterized in that the sterilization is killed using fungicide immersion
Bacterium.
9. wild Dendrodium propagation method according to claim 1, which is characterized in that the wild Dendrodium includes golden hairpin stone
Dry measure used in former times, the big luxuriant sheath stem of noble dendrobium, Dendrobium crepidatum lindl et paxt., swollen arthrolith dry measure used in former times, Dendrobium loddigesii, dendrobium candidum, dendrobium moniliformeSweet and Herba Dendrobii.
10. wild Dendrodium propagation method according to claim 1, which is characterized in that the wild Dendrodium be HERBA DENDROBII,
The big bud sheath stem of noble dendrobium or Dendrobium crepidatum lindl et paxt..
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110301303A (en) * | 2019-07-04 | 2019-10-08 | 广西壮族自治区农业科学院花卉研究所 | The cultural method of officinal dendrobium stem plantation bag and dendrobium candidum |
| CN110771485A (en) * | 2019-10-22 | 2020-02-11 | 丽水学院 | Dendrobium officinale cultivation method for rooting and promoting germination by tying trees |
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| EP1704769A1 (en) * | 2003-12-26 | 2006-09-27 | Noriko Takeda | Method of growing plant, water freshness preserver, root rot inhibitor, plant medium, water holder, bactericide, herbicide, heat insulator, improver, deodorizer and water-freshness preservation |
| CN101653096A (en) * | 2008-08-20 | 2010-02-24 | 汉中植物研究所 | Method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum |
| CN102657097A (en) * | 2012-06-02 | 2012-09-12 | 上海市闵行区农业科学研究所 | In-vitro culture method for tender stem segments of dendrobium nobile |
| CN103598091A (en) * | 2013-10-31 | 2014-02-26 | 界首市联发种植专业合作社 | Breeding technique of dendrobium |
| CN106106103A (en) * | 2016-07-11 | 2016-11-16 | 广西壮族自治区林业科学研究院 | Scribbling sketch Herba Dendrobii old stem cuttage breeding method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110301303A (en) * | 2019-07-04 | 2019-10-08 | 广西壮族自治区农业科学院花卉研究所 | The cultural method of officinal dendrobium stem plantation bag and dendrobium candidum |
| CN110301303B (en) * | 2019-07-04 | 2021-11-26 | 广西壮族自治区农业科学院 | Dendrobium officinale cultivation bag and cultivation method of dendrobium officinale |
| CN110771485A (en) * | 2019-10-22 | 2020-02-11 | 丽水学院 | Dendrobium officinale cultivation method for rooting and promoting germination by tying trees |
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