CN103598091A - Breeding technique of dendrobium - Google Patents

Breeding technique of dendrobium Download PDF

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Publication number
CN103598091A
CN103598091A CN201310525536.0A CN201310525536A CN103598091A CN 103598091 A CN103598091 A CN 103598091A CN 201310525536 A CN201310525536 A CN 201310525536A CN 103598091 A CN103598091 A CN 103598091A
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CN
China
Prior art keywords
stem
dendrobium
seedlings
cut
plant
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CN201310525536.0A
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Chinese (zh)
Inventor
程世忠
Original Assignee
界首市联发种植专业合作社
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Application filed by 界首市联发种植专业合作社 filed Critical 界首市联发种植专业合作社
Priority to CN201310525536.0A priority Critical patent/CN103598091A/en
Publication of CN103598091A publication Critical patent/CN103598091A/en

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Abstract

The invention relates to a breeding technique of dendrobium. According to the breeding technique, an annual dendrobium plant is taken, and is cut into stem segments with a length of 1 to 2cm, wherein each stem segment possesses 1 to 2 axillary buds; the stem segments are washed with tap water, are immersed in 75% alcohol for 20 to 40s, are sterilized with 0.1% mercuric chloride for 10min, are washed with sterile water, and are cultured so as to obtain seedlings; when the length of the seedlings reaches 2 to 4cm, and each seedling possesses 2 to 4 euphyllas, the seedlings are transferred into a root media for rooting culture; and when the length of the root of the seedlings reaches 3 to 5cm, the seedlings are transplanted to a mixed medium made from dried branches and cobblestones for plantation. According to the breeding technique, adoption of asexual reproduction is capable of solving problems that dendrobium breeding and plantation are difficult, scaled production is realized, market demands are satisfied, and enormous economic benefit is obtained.

Description

A kind of growing dendrobium seedlings technology
Technical field
The invention belongs to botanical seedling culturing technology, be specifically related to the seedling growing process of the stem of noble dendrobium.
Background technology
Stem of noble dendrobium another name bracketplant, flat grass, yellow grass etc.Derive from the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium.Be distributed in province, the autonomous regions such as Sichuan, Guizhou, Yunnan, Guangdong, Guangxi, Taiwan.Dendrobium Sw has 300Duo Zhong, China to distribute more than 70 to plant, wherein more than 20 plant medicinal.Mostly be wild.With stem, be used as medicine, contain the multiple alkaloid such as dendrobine, dendramine, shihunine and lymphatic temperament, starch etc.Taste is sweet, light, cold nature; There are tonifying-Yin and nourishing-stomach, clearing heat and promoting fluid function.Cure mainly disease thermal burn Tianjin, dry mouth yearningly, abnormal heat after being ill.Recent research proof dendrobium polysaccharide has the effect that strengthens immunity.Due to ecological disruption and exhaustive exploitation, wild Dendrodium is endangered, and its market price is more and more expensive, is described as " plant panda ", " plant gold ", and within 1987, State Council classifies the stem of noble dendrobium as focused protection object as.
The stem of noble dendrobium is liked shady and cool moist environment, often grows nonparasitically upon another plant on the limestone that has bryophyte or on tree.In southwest, cultivate in 1 000 meters of left and right of height above sea level, annual precipitation more than 1 000 millimeters relative air humidity 80% left and right, 17 ℃ of above areas of average temperature of the whole year more.
Stem of noble dendrobium plant belongs to slow raw plant, and annual institute sends out that sprouting quantity is few, amount of growth is little, and the stem of noble dendrobium is harsher to growing environment requirement, and plant is short and small, and output is lower, adds going from bad to worse of ecotope, and wild Dendrodium is bred very slow, natural updating ability extreme difference.Now much about the research of growing dendrobium, but because planting conditions is limited, growth time is longer, develop slowlyer, and survival rate is low, to breeding of the stem of noble dendrobium, has brought very large difficulty.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of growing dendrobium seedlings technology.
The invention provides a kind of growing dendrobium seedlings technology, by getting then raw stem of noble dendrobium plant, being cut into length is the stem section of 1~2cm, in each stem section with 1~2 axillalry bud, with running water, rinse well, put into 75% alcohol and soak 20~40s, again with 0.1% mercuric chloride sterilization 10min, finally use aseptic water washing 5~8 times, on superclean bench, above-mentioned ready stem section is put into sterilized minimal medium, carry out cultured in vitro, treat to grow sprouting in stem section, sprouting is cut, be seeded in and in medium, grow up to Multiple Buds, Multiple Buds is cut into single seedling to be proceeded to cultivate, treat that single seedling grows to 2~4cm, while thering is 2~4 true leaves, forwarded to and in root media, carried out culture of rootage, when root system grows to 3~5cm, plant culture transferring is planted to the mixed-matrix of dry branch and stone.
Branch of the present invention adopts pine bark and limb, and stone adopts cobblestone, is conducive to growth and the breathing of stem of noble dendrobium root system, promotes exposed aerial root in air, to absorb nutrient and moisture content.
In the present invention, the growing environment of the stem of noble dendrobium will keep shady and cool moistening, and relative air humidity is 70~80%, is conducive to the growth of the stem of noble dendrobium.
The invention has the beneficial effects as follows: the present invention can improve stem of noble dendrobium breeding difficulty; endangered present situation; in conjunction with vegetative propagation technique, effectively cultivate rapidly good stem of noble dendrobium plant, overcome the susceptible problem of stem of noble dendrobium seedlings root; the immunity of the stem of noble dendrobium, the ability of antiviral and drought resisting have been strengthened; guarantee survival rate, formed large-scale production, met the market demand; produce huge economic benefit, there is good application and DEVELOPMENT PROSPECT.
Embodiment
The present invention is further described in detail below, and illustrated embodiment is only for explaining the present invention, and non-limiting scope of the present invention.
First, get then raw stem of noble dendrobium plant, being cut into length is the stem section of 1~2cm, in each stem section with 1~2 axillalry bud, with running water, rinse well, put into 75% alcohol and soak 20~40s, again with 0.1% mercuric chloride sterilization 10min, finally use aseptic water washing 5~8 times, on superclean bench, above-mentioned ready stem section is put into sterilized minimal medium, carry out cultured in vitro, treat to grow sprouting in stem section, sprouting is cut, be seeded in and in medium, grow up to Multiple Buds, Multiple Buds is cut into single seedling to be proceeded to cultivate, treat that single seedling grows to 2~4cm, while thering is 2~4 true leaves, forwarded to and in root media, carried out culture of rootage, when root system grows to 3~5cm, plant culture transferring is planted to the mixed-matrix of dry branch and stone, dry branch in above-mentioned mixed-matrix and stone adopt respectively pine bark and limb and cobblestone, and it is shady and cool moistening that the growing environment of the stem of noble dendrobium will keep, relative air humidity is 70~80%.

Claims (3)

1. a growing dendrobium seedlings technology, it is characterized in that: get then raw stem of noble dendrobium plant, being cut into length is the stem section of 1~2cm, in each stem section with 1~2 axillalry bud, with running water, rinse well, put into 75% alcohol and soak 20~40s, again with 0.1% mercuric chloride sterilization 10min, finally use aseptic water washing 5~8 times, on superclean bench, above-mentioned ready stem section is put into sterilized minimal medium, carry out cultured in vitro, treat to grow sprouting in stem section, sprouting is cut, be seeded in and in medium, grow up to Multiple Buds, Multiple Buds is cut into single seedling to be proceeded to cultivate, treat that single seedling grows to 2~4cm, while thering is 2~4 true leaves, forwarded to and in root media, carried out culture of rootage, when root system grows to 3~5cm, plant culture transferring is planted to the mixed-matrix of dry branch and stone.
2. a kind of growing dendrobium seedlings technology according to claim 1, is characterized in that: above-mentioned dry branch adopts pine bark and limb, and stone adopts cobblestone.
3. a kind of growing dendrobium seedlings technology according to claim 1, is characterized in that: the growing environment of the stem of noble dendrobium will keep shady and cool moistening, and relative air humidity is 70~80%.
CN201310525536.0A 2013-10-31 2013-10-31 Breeding technique of dendrobium CN103598091A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104145795A (en) * 2014-08-15 2014-11-19 湖南省农业信息与工程研究所 Dendrobe cultivation method for improving facility utilization rate
CN108739321A (en) * 2018-05-28 2018-11-06 四川省植物工程研究院 A kind of wild Dendrodium propagation method
CN108812267A (en) * 2018-06-08 2018-11-16 霍山五桂峡石斛有限公司 A kind of dendrobium nobile non-polluted planting method
CN109169291A (en) * 2018-11-14 2019-01-11 遵义三湾田种植有限公司 A kind of growing dendrobium seedlings technology

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100050825A (en) * 2008-11-06 2010-05-14 강경원 New plant variety of 'sul kuk'
CN101855993A (en) * 2009-04-11 2010-10-13 云南省德宏热带农业科学研究所 Dendrobium devonianum clone seedling reproduction method
CN102613086A (en) * 2012-03-31 2012-08-01 南京农业大学 Hormone-free tissue culture method for dendrobium candidum
CN103229718A (en) * 2013-04-24 2013-08-07 浙江韵芝堂生物科技有限公司 Tissue culture rapid propagation method of Dendrobium officinale

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100050825A (en) * 2008-11-06 2010-05-14 강경원 New plant variety of 'sul kuk'
CN101855993A (en) * 2009-04-11 2010-10-13 云南省德宏热带农业科学研究所 Dendrobium devonianum clone seedling reproduction method
CN102613086A (en) * 2012-03-31 2012-08-01 南京农业大学 Hormone-free tissue culture method for dendrobium candidum
CN103229718A (en) * 2013-04-24 2013-08-07 浙江韵芝堂生物科技有限公司 Tissue culture rapid propagation method of Dendrobium officinale

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
卢艳艳等: "铁皮石斛栽植技术研究", 《北方园艺》 *
张红梅等: "铁皮石斛茎段快繁技术研究", 《陕西农业大学学报(自然科学版)》 *
朱艳等: "铁皮石斛茎段诱导丛生芽的研究", 《中国野生植物资源》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104145795A (en) * 2014-08-15 2014-11-19 湖南省农业信息与工程研究所 Dendrobe cultivation method for improving facility utilization rate
CN104145795B (en) * 2014-08-15 2016-08-24 湖南省农业信息与工程研究所 A kind of dendrobe cultivation method improving facility utilization rate
CN108739321A (en) * 2018-05-28 2018-11-06 四川省植物工程研究院 A kind of wild Dendrodium propagation method
CN108812267A (en) * 2018-06-08 2018-11-16 霍山五桂峡石斛有限公司 A kind of dendrobium nobile non-polluted planting method
CN109169291A (en) * 2018-11-14 2019-01-11 遵义三湾田种植有限公司 A kind of growing dendrobium seedlings technology

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