CN112730000A - Automatic change and drip dyeing mounting equipment - Google Patents
Automatic change and drip dyeing mounting equipment Download PDFInfo
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- CN112730000A CN112730000A CN202011601497.4A CN202011601497A CN112730000A CN 112730000 A CN112730000 A CN 112730000A CN 202011601497 A CN202011601497 A CN 202011601497A CN 112730000 A CN112730000 A CN 112730000A
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- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
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Abstract
The utility model provides an automatic change and drip dyeing mounting equipment, including automatic drip dyeing module and automatic mounting module, automatic drip dyeing module includes that the fluid middle-end device, fluid end device, carrier dish and carrier dish accept the tray utensil, the carrier dish is used for bearing the slide glass, the carrier dish is accepted the tray utensil by the carrier dish and is accepted and keep, the fluid middle-end device passes through the fluid pipeline with fluid end device and links to each other, the fluid middle-end device distributes the dyeing liquid of external input to fluid end device, fluid end device is last to have liquid outlet and waste liquid recovery hole, the dyeing liquid is exerted on the slide glass that the carrier dish bore by the liquid outlet, and after dyeing the sample on the slide glass with negative pressure suction mode through waste liquid recovery hole recovery to a negative pressure chamber. The automatic dripping dyeing module improves the dyeing processing efficiency of the samples, reduces labor intensity, and simultaneously reduces the possibility of cross contamination among the samples, so that the sample processing process has consistency and controllability.
Description
Technical Field
The invention relates to an automatic drip-dyeing mounting technology for biological tissue sample slices, in particular to automatic drip-dyeing mounting equipment.
Background
The automatic staining treatment technology of biological tissue sample slices is an important step in the whole automatic staining, drying and sealing process of the biological tissue sample, and is an indispensable step, so that the treated biological tissue sample has a convenient observed appearance, the consistency and the high efficiency of the treatment process can be kept, and the staining step is an object which must be strictly controlled in the treatment process of the biological tissue sample.
The purpose of staining is to make different structures within the tissue appear differently colored for easy viewing. The classic Hematoxylin and Eosin staining method is the conventional staining of histological specimens and pathological section specimens, referred to as HE staining for short. After staining, the nuclei were stained violet-blue with hematoxylin, and most of the cytoplasmic and acellular components were stained pink with eosin.
Various techniques may be used to analyze biological samples. Examples of analytical techniques include microscopy, microarray analysis (e.g., protein and nucleic acid microarray analysis), and mass spectrometry. Preparing samples for these and other types of analysis typically includes: the sample is contacted with a series of treatment liquids. Some of these treatment liquids (e.g., staining and counterstaining reagents) may add color and, in contrast or otherwise, alter the visual characteristics of sample components (e.g., at least some types of cells and intracellular structures) that are not visible or difficult to see. Other treatment liquids (e.g., de-paraffinized liquids) may be used to achieve other treatment objectives. If multiple processing liquids are used to process the sample, the application and subsequent removal of the various processing liquids may be important to produce a sample suitable for analysis. In some cases, treating the sample with a plurality of treatment liquids comprises: the processing liquid is manually applied to the microscope slides that each carry a sample. This method of processing samples tends to be labor intensive and inaccurate.
The pathological section staining process by manual work is often characterized by large time consumption, easy error, difficult batch processing and the like, and the pathological section samples can be automatically processed in batches by using an automatic staining machine.
Automatic immersion dyeing machines are available, which can replace manual dyeing. These machines automatically process samples by immersing racks carrying microscope slides in open baths of processing liquid. Unfortunately, the operation of immersion method machines inevitably results in the movement of the rack carrying the microscope slides from one bath to another, leading to the intersection of the liquids of the different baths. Over time, this movement causes degradation of the treatment liquid. Also, when the sample is immersed in a common bath, there is a possibility of cross-contamination. For example, cells may leave a specimen on one slide and be transported to another slide in a common bath. This form of contamination greatly reduces the accuracy of certain types of sample analysis. To alleviate this problem and to address the degradation of the treatment liquid caused by movement, it is often necessary to frequently replace the bath of treatment liquid in the immersion method machine. As a result, these machines tend to consume relatively large volumes of treatment liquid, which increases the costs associated with operating these machines. These open baths of treatment liquids are prone to evaporative loss and oxidative degradation of some of the treatment liquid components. For example, oxidation of certain components of the staining reagent can alter the staining properties of these components and thus reduce the precision of the staining operation.
Most automatic dyeing machines are large in size, sample slides are fixed, all modules are operated alternately corresponding to the same number of three-dimensional moving machines, mechanical control is complex, the types of liquid reagents cannot be excessive, or alternative nozzles are adopted, but cleaning is time-consuming and liquid drops of the last operation remain. The human-computer interaction is poor, biological tissue slices can only be processed according to a fixed mode, the oriented setting accuracy or high-efficiency or batch mode and the like cannot be selected according to requirements, different dyeing programs cannot be selected corresponding to different tissue materials, and real-time supervision and feedback in the dyeing process are lacked.
In the automated drop-dyeing mounting process, how to efficiently heat a plurality of slides on a carrier plate at a uniform and stable temperature at the same time is a problem to be solved.
It is to be noted that the information disclosed in the above background section is only for understanding the background of the present application and thus may include information that does not constitute prior art known to a person of ordinary skill in the art.
Disclosure of Invention
The invention mainly aims to overcome the problems in the background technology and provide an automatic drop dyeing mounting device.
In order to achieve the purpose, the invention adopts the following technical scheme:
an automatic drip-dyeing mounting device comprises an automatic drip-dyeing module for automatically dyeing a biological tissue sample slide glass and an automatic mounting module for mounting the dyed slide glass, the automatic drop dyeing module comprises a fluid middle-end device, a fluid tail-end device, a carrier disc and a carrier disc bearing support tool, the carrier plate is used for bearing glass slides and is supported and kept by the carrier plate supporting and holding device, the fluid middle-end device is connected with the fluid end device through a fluid pipeline, the fluid middle-end device distributes externally input dyeing liquid to the fluid end device, the fluid end device is provided with a liquid outlet and a waste liquid recovery hole, the dyeing liquid is applied to the glass slide carried by the carrier disc from the liquid outlet, and after the sample on the glass slide is dyed, the sample is recovered to a negative pressure chamber through the waste liquid recovery hole in a negative pressure suction mode.
In some embodiments, the automatic drip dyeing module further comprises a temperature maintaining device for maintaining the temperature in the dyeing device within a set temperature range or value.
In some embodiments, an automated apparatus for dispensing a liquid onto a biological tissue sample on one or more microscope slides that can perform processing operations on the slides bearing the biological tissue sample to achieve fully automated staining of the biological tissue sample, the apparatus comprising: a fluid middle-end device comprising a central common chamber; a fluid tip device comprising a fluid tip head assembly; a carrier tray receiving tray comprising a support; a carrier tray rotational drive mechanism comprising a motor coupled to the carrier tray carrier and configured to rotate the carrier tray carrier to position a slide relative to the fluid end device; a fluid line, a fluid switch, a pump, and a temperature maintenance device may also be included.
Some embodiments may store liquid from a liquid source where the storage is not a long term storage. When the liquid is stored, a negative pressure state is formed in the central common chamber, and the liquid in the pipeline is forced to flow into the central common chamber under the action of the atmospheric pressure.
Some embodiments may dispense stored liquid. When liquid is dispensed, the liquid in the central common chamber can be pumped away under the action of the pump.
Some embodiments may process a specimen carried by a slide. The treatment process comprises the following steps: liquid pumped from the central common chamber by the pump is delivered to the slide. The liquid coming out will form a liquid pool due to the effect of surface tension. The collected liquid has an at least partially retained shape and completely covers the sample due to surface tension. The liquid is then removed from the specimen and the volume of liquid remaining on the slide is at least ten percent less than the previously mentioned volume of the collected liquid to expose the specimen for cleaning and subsequent staining. The above operations may be repeated and the applied liquid may be of different kinds.
Some embodiments control the pump's operating power during the application of the liquid so that the liquid does not splash when it reaches the slide after leaving the fluid tip device.
In some embodiments, the liquid remaining in the central common chamber may be recovered. The recovery method comprises the following steps: a pipe which is straight to the bottom of the cavity is arranged in the central common cavity, negative pressure is formed in the pipe, and the air pressure in the central common cavity is kept balanced with the atmospheric pressure. The remaining liquid in the central common chamber is forced into the conduit by the gas pressure, leaving the central common chamber.
A method of processing a specimen on an upper surface of a microscope slide comprising: the microscope slide is moved to a processing position. Moving a microscope slide to a processing position refers to receiving a tray carrying the microscope slide from the outside and moving the tray to a position such that the slide carried on the tray is facing the fluid end device. By directly facing is meant that the sample on the slide is located just below the fluid outlet with a positional error within a 3mm radius. The central axis of the longer length of the slide is toward the center of the carrier plate. The shorter length of the slide coincides with the width of the fluid tip device. After the sample processing on the previous microscope slide is completed, the next microscope slide can reach the location.
A method of processing a specimen on a microscope slide comprising: reagent is dispensed from an outlet of the fluid dispensing mechanism opposite the upper surface of the microscope slide, forming a layer of reagent in contact with the sample at the mounting area.
In some embodiments, the staining apparatus comprises one or more fluid conduits and a fluid tip device, the fluid tip device being stationary. The fluid tip device may be coupled to the fluid center device and configured to dispense reagents from one or both of the fluid center device. Meanwhile, the fluid end device has the functions of removing liquid and pumping. In some embodiments, the fluid tip device may be configured to blow air to facilitate movement of liquid to the low pressure region. In some embodiments, a fluid tip device comprises: a fluid inlet, a waste outlet, a waste recovery hole, a gas inlet, and a common manifold. The fluid may be delivered through a manifold and dispensed from a head of a fluid tip device.
In some embodiments, the staining apparatus is configured to receive microscope carrier trays carrying microscope slides from a transporter apparatus. The stainer module includes: the microscope carrier disc holder, the rotating device and the linear motion device. The microscope carrier disc retainer can drive the microscope carrier disc to rotate and can control the rotating angle and precision. The control of the positional accuracy of the microscope carrier plate is already achieved on the transport mechanism, and when the staining starts with the reception of the microscope carrier plate from the transporter device, the control of the positional accuracy is transferred to the staining device.
In some embodiments, a method of processing a sample on a microscope slide comprises: a liquid is applied to the slide and a high pressure gas is directed toward the upper surface of the slide to diffuse the applied liquid toward the two weeks of the slide.
In some embodiments, a method of processing a sample on a microscope slide comprises: the temperature of the whole dyeing device can be controlled within a certain range, so that the temperature of the sample is kept approximately constant in the dyeing process. The method of controlling the temperature is to create a steady flow of gas at a constant temperature within the apparatus, particularly on the microscope carrier plate, which can be achieved by a heating device and a fan.
In some embodiments, a method of processing a sample on a microscope slide comprises: the fluid end device is checked for a slide underneath and can be detected by a scanner. If there is no slide, no staining procedure is performed.
In a particular embodiment, a method of processing a sample on a microscope slide includes: the slides are transported into a stainer module. A liquid is applied to the slide to bring the sample into contact with the liquid. The liquid is blown along and removed from the upper surface of the slide. The slide can then be removed from the stainer module.
The invention has the following beneficial effects:
the invention provides automatic drip-dyeing mounting equipment for biological tissue sample slices, which can carry out drip-dyeing mounting processing operation on a glass slide bearing a biological tissue sample, wherein an automatic drip-dyeing module improves the dyeing processing efficiency of the sample, reduces labor intensity, effectively reduces the possibility of cross contamination among samples, and ensures that the sample processing process has consistency and controllability. The invention is beneficial to realizing the automatic dyeing treatment of the full-automatic biological tissue sample with batch, high efficiency, simplicity and accuracy.
In a preferred embodiment, the invention provides a heat treatment module for an automatic dispensing and mounting apparatus, which is configured to form a semi-closed cavity together with a carrier plate carrying a plurality of slides after being engaged with the carrier plate, and a plurality of heating resistors and blower blades are circumferentially distributed in the cavity, so that air in the cavity is heated, and the blower blades are driven to rotate to form annular heat convection in the cavity, thereby uniformly heating the slides on the carrier plate by convection, and thus, the invention can efficiently heat the slides on the carrier plate at a uniform and stable temperature.
In an optimal scheme, the automatic drop dyeing mounting equipment has an all-in-one machine structure, wherein a rack is provided with a heat treatment module, an automatic drop dyeing module and an automatic mounting module in a distributed layout mode, the rack is provided with a carrying and transferring module with an X-axis guide rail and a Y-axis guide rail, the carrying and transferring module is matched with the space operation mode of each module distributed on the rack, and a carrying and transferring module conveys a carrier disc to each module for automatic flow treatment, so that the drop dyeing mounting treatment efficiency is effectively improved, and the volume and the occupation of the whole set of process treatment equipment are also obviously reduced. Through the relative motion mechanism that the conveying module moves the sample glass slides among the processing modules on the rack, the complex mechanical alternate operation caused by conveying the glass slides among the modules is reduced, and the complexity of mechanical control is effectively reduced. Through the structural automatic distributed assembly line that realizes at an all-in-one, reached the directional, batch processing, accurate, high-efficient, pollution-free, save the effect of reagent, and can also realize customization and the adjustment of artifical directional processing route as required simply conveniently.
The invention constructs an automatic processing system for executing one or more slide processing operations on the slide bearing the biological specimen, can carry out directional batch processing on various biological tissue sample slices, and achieves the effects of accuracy, high efficiency, no pollution and reagent saving. The system can provide high sample throughput while also maximizing the possibility of reducing or limiting slide cross-contamination.
The automatic drip-dyeing mounting device can also provide a human-computer interaction function, realize a user-defined processing mode, and can monitor and feed back when processing the tissue slices.
Drawings
FIG. 1A is a front view of an all-in-one machine for an automated drip-staining processing system for biological tissue sample slices according to an embodiment of the present invention;
FIG. 1B is a left side view of an all-in-one machine configuration for an automated drip-staining processing system for biological tissue sample slices according to an embodiment of the present invention;
FIG. 1C is a top view of an integrated machine for an automated drip-staining processing system for biological tissue sample slices according to an embodiment of the present invention;
fig. 2A is a structural view of a carrying and transferring module according to an embodiment of the present invention;
fig. 2B is a bottom view of a carrier transfer module according to an embodiment of the present invention;
fig. 2C is a top view of a carrier transfer module according to an embodiment of the present invention;
fig. 2D is a schematic view of a method of transporting a carrier plate for carrying a transfer module according to an embodiment of the present invention;
FIG. 3A is a top view of a carrier plate according to an embodiment of the present invention;
FIG. 3B is a bottom view of a carrier plate according to an embodiment of the present invention;
FIG. 4 is a schematic view of an inlet carrier transfer module according to one embodiment of the present invention;
FIG. 5 is a block diagram of a portion of a human-computer interaction module in accordance with an embodiment of the present invention:
fig. 6 is a perspective view of an automatic drop dyeing module according to an embodiment of the present invention.
Fig. 7A is an isometric view of a fluid middle-end device according to an embodiment of the invention.
Fig. 7B is a top view of a fluidic center device according to an embodiment of the present invention.
Figure 7C is an isometric view of a central common chamber of an embodiment of the present invention.
Figure 7D is a top view of a central common chamber of one embodiment of the present invention.
FIG. 7E is a cross-sectional view B-B of the central common chamber of one embodiment of the present invention.
Fig. 7F is an isometric view of a fluid tip device according to an embodiment of the invention.
Fig. 7G is a bottom view of a fluid tip device according to an embodiment of the present invention.
Fig. 7H is a front view of a fluid tip device according to one embodiment of the invention.
Fig. 7I is a stepped cross-sectional view G-G in an elevation view of a fluid tip device according to an embodiment of the present invention.
Fig. 7J is a cross-sectional view at the center of a fluid tip device according to one embodiment of the present invention.
Figure 8A is an isometric view of a dyeing module portion apparatus according to one embodiment of the invention.
Fig. 8B is an isometric view of a carrier tray receiving tray and power device according to an embodiment of the invention.
Fig. 8C is a front view of a carrier tray receiving tray and power device in accordance with one embodiment of the present invention.
FIG. 9A is a block diagram of a thermal processing module according to one embodiment of the invention;
FIG. 9B is a front view of a thermal processing module according to one embodiment of the present invention;
FIG. 9C is a schematic view of the interior of the cavity half of the thermal processing module in accordance with one embodiment of the present invention;
FIG. 9D is a diagram of a structure of a heating portion of a thermal processing module according to an embodiment of the present invention;
FIG. 9E is a diagram of a structure of a heating portion of a thermal processing module according to an embodiment of the present invention;
FIG. 9F is a schematic view of the operation of the holder in the thermal processing module according to one embodiment of the present invention;
FIG. 10 is a block diagram illustrating the overall structure of an automated drip-staining processing system for biological tissue sample slices according to an embodiment of the present invention;
FIG. 11 is a block diagram of a human-computer interaction control system according to an embodiment of the present invention;
fig. 12 is a schematic diagram of a human-computer interaction control automatic drip-staining processing system for biological tissue sample slices according to an embodiment of the invention.
Detailed Description
The embodiments of the present invention will be described in detail below. It should be emphasized that the following description is merely exemplary in nature and is not intended to limit the scope of the invention or its application.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element. In addition, the connection may be for either a fixed or coupled or communicating function.
It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the embodiments of the present invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed in a particular orientation, and be in any way limiting of the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the embodiments of the present invention, "a plurality" means two or more unless specifically limited otherwise.
Automatic drip dyeing module and automatic drip dyeing mounting equipment with same
Referring to fig. 6 to 8C, in one embodiment, an automatic drop staining mounting apparatus includes an automatic drop staining module for automatically staining a biological tissue sample slide and an automatic mounting module for mounting stained slides, the automatic drop staining module includes a fluid middle device 2200, fluid end devices 2300A to 2300D, a carrier tray 401 and a carrier tray receiving tray 2401, the carrier tray 401 is used for carrying slides, the carrier tray 401 is held by the carrier tray receiving tray 2401, the fluid middle device 2200 is connected with the fluid end devices 2300A to 2300D through fluid pipelines, the fluid middle device 2200 serves as a fluid splitter for distributing externally input staining liquid to the fluid end devices 2300A to 2300D, the fluid end devices 2300A to 2300D have a liquid outlet and a waste liquid recovery hole 2304, the staining liquid is applied to the slides carried by the carrier tray 401 from the liquid outlet and is recovered to a negative pressure chamber (not shown) through the effluent collection well 2304 by negative pressure suction after staining of the samples on the slides is completed.
In a preferred embodiment, a power device is further included, the power device including a take-up tray rotational drive mechanism 2400 and a take-up tray lift mechanism 2500, the take-up tray rotational drive mechanism 2400 coupled to the carrier tray take-up tray 2401 for driving the carrier tray take-up tray 2401 to rotate in a working position to position a slide to be stained relative to the fluid end device 2300A-2300D; preferably, the receiving tray lifting mechanism 2500 is further used for moving the carrier tray receiving tray 2401 up and down between the receiving position of the carrier tray and the drip dyeing processing position.
In a preferred embodiment, the fluid end unit 2300A-2300D comprises a liquid inlet header 2301 and a high pressure gas connector 2303 connected to the liquid outlet via a common manifold 2308, the liquid inlet header 2301 is connected to the fluid middle unit 2200 via a fluid line, the high pressure gas connector 2303 is used for introducing high pressure gas during waste liquid recovery, and a plurality of waste liquid recovery holes 2304 are distributed around the liquid outlet. The high pressure gas forces liquid on the slide to spread from the exit port toward effluent port 2304 and forces liquid in the channel to exit fluid tip assembly 2300A-2300D from inlet header 2301 and back to fluid tip assembly 2200.
In a preferred embodiment, the liquid outlet comprises a dropping needle 2305, and a plurality of waste liquid recovery holes 2304 are distributed in a row on both sides of the dropping needle 2305.
In a preferred embodiment, pumps 2800A-2800D are also included between the fluid middle end device 2200 and the fluid end devices 2300A-2300D to apply motive forces to the liquid.
In a preferred embodiment, the fluid middle-end device 2200 includes a liquid storage chamber (e.g., a central common chamber 2202) that provides a fluid inlet for inputting a staining liquid, a fluid outlet for dispensing liquid to the fluid end device 2300A-2300D, and at least one of: a negative pressure suction port for forming a negative pressure in the liquid storage chamber to input a staining liquid, a liquid overflow port for overflowing the liquid when the liquid input is excessive, an atmospheric connection port for communicating the atmosphere when the liquid is dispensed to the fluid end device 2300A-2300D, and a discharge port for discharging the liquid after the staining of the sample is completed, preferably, the negative pressure suction port, i.e., the discharge port.
In a preferred embodiment, the liquid storage chamber is provided with a plurality of fluid inlets.
In a preferred embodiment, one or more of said fluid inlets is provided with a fluid switch.
In the preferred embodiment, the fluid middle-end device 2200 comprises a combination of a top 2203, at least one waist 2204 and a bottom 2205 stacked together in a square shape, the fluid storage chamber is formed by the combination, a plurality of sides of the top 2203 and a bottom connected with the waist 2204 are respectively provided with openings, a plurality of sides of the at least one waist 2204 are respectively provided with openings, a top surface of the waist 2204 connected with the top 2203 is provided with an opening, a bottom surface of the waist 2204 connected with the bottom 2205 is provided with an opening, namely a fluid outlet 2206A, a plurality of sides of the bottom 2205 and a top surface connected with the waist 2204 are respectively provided with openings, an opening of the top 2203 is used for liquid or air, and an opening of the waist 2204 and an opening of the bottom 2205 are used for liquid.
In a preferred embodiment, the fluid middle-end 2200 is provided with a negative pressure conduit 2207, and the negative pressure conduit 2207 is connected with the negative pressure suction port and extends to the bottom of the liquid storage chamber.
In a preferred embodiment, a temperature maintenance device 2900 is also included for maintaining the temperature within the dyeing device at a set temperature range or value.
Specific embodiments are described in further detail below.
An automatic drip staining module for a section of a biological tissue sample comprising: fluid lines (not shown), fluid middle end device 2200, fluid end devices 2300A-2300D, fluid switches, carrier tray receiving holder 2401, receiving holder rotational drive mechanism 2400, receiving holder lift mechanism 2500, temperature maintenance device 2900, pumps 2800A-2800E, and rack 2600. Fluid lines connect an external source of liquid (e.g., a liquid storage device) to the fluid end-device 2200, the fluid lines connect the fluid end-device 2200 to the fluid end-devices 2300A-2300D, and pumps 2800A-2800D are included between the fluid end-device 2200 and the fluid end-devices 2300A-2300D. The fluid line includes a liquid conduit (preferably a flexible, corrosion resistant conduit), a fluid switch, and a port. The fluidic medium device 2200 includes a fluidic switch, a central common chamber, and an interface. The end-of-fluid device 2200 can store and dispense liquids. Fluid end devices 2300A-2300D include a plurality of ports, waste recovery apertures 2304, and a plurality of manifolds, enabling liquid application, waste recovery, and blowing operations. The carrier tray receiving holder 2401 can drive the carrier tray to rotate and translate. The temperature maintaining device can maintain the temperature in the dyeing device to be approximately constant, and the best dyeing effect is realized. The frame holds the various sections.
The dyeing main process comprises the following steps: the fluid middle-end device 2200 dispenses externally inflowing liquids to the fluid end-devices 2300A-2300D. The liquid is delivered to the slides of carrier disk 401 held by carrier disk receiving tray 2401 by fluid end devices 2300A-2300D. After the task of the fluid is completed, the fluid is again recovered by the fluid end devices 2300A-2300D. The positions of the fluid lines, fluid middle end device 2200, and fluid end devices 2300A-2300D are typically held stationary relative to the floor, and the carrier tray receiving holder 2401 rotates the carrier tray 401 to effect a one-by-one staining of the specimen on each slide. The carrier tray receiving tray 2401 can receive the carrier tray 401 when opened and is in an operating state when closed. The temperature maintaining device keeps the temperature in the dyeing device approximately constant.
In FIG. 6, 2200 shows a fluid medium-end device, and 2300A-2300D show a fluid end device. The dyeing module can be provided with a plurality of fluid pipelines, and each fluid pipeline only contains one liquid. The fluid pipeline is inserted with a fluid switch which can be a solenoid valve and controls the liquid to enter the fluid middle-end device. The dyeing module can be provided with a fluid middle-end device, a plurality of fluid inlets are distributed on the device to receive liquid from the outside, and a fluid switch is arranged on each inlet and can be an electromagnetic valve; the device has at least one fluid outlet, which may be provided without a switch. The fluid middle-end device can be regarded as a liquid transfer station. The fluid middle-end device is provided with a liquid storage chamber. The staining module has at least one fluid end device therein. The fluid end device is connected with the fluid middle end device through a fluid pipeline. The fluid end device is in fluid communication with the fluid middle device. When a switch in a fluid pipeline in which a certain liquid exists is opened, the liquid flows into the fluid middle-end device, so that the storage cavity of the fluid middle-end device is filled with the liquid. To allow liquid to flow into the fluidic medium device, the air pressure in the storage chamber of the fluidic medium device may be reduced in some way. This may be done by using a pump 2800E to draw air away from the chamber. When the liquid amount in the storage chamber reaches a certain standard, the switch in the fluid pipeline where the liquid exists is closed, and the liquid is stopped from entering. In order to prevent the fluid middle-end device from sucking excessive liquid, a liquid overflow outlet is arranged in the fluid middle-end device. When the fluid end device is activated, liquid in the storage chamber flows out of the outlet of the fluid end device through the fluid end device onto a slide carried by the carrier plate 401. In order to make the liquid have certain power, a pump is arranged on a pipeline between the fluid middle-end device and the fluid end device to apply power to the liquid. During this process, the air pressure in the fluid storage chamber of the fluid center device is maintained equal to atmospheric pressure by some means. When the amount of liquid on the slide reaches a desired level, the fluid end device changes operating conditions to move the fluid end device and the liquid in the fluid line between the fluid end device and the fluid middle device into the storage chamber of the fluid middle device. The liquid in the storage chamber is discharged by a tube. After a certain period of time, the fluid end device removes the liquid from the slides carried by the carrier tray 401 and delivers it to a waste collection (e.g., waste reservoir). When the task of the liquid is finished, the liquid middle-end device discharges the liquid which finishes the task out of the storage chamber, and the liquid is discharged out of the dyeing module through the corresponding fluid pipeline.
Fig. 7A is an isometric view and fig. 7B is a top view of a fluid center device. 2201A-2201L are fluid switches, which may be solenoid valves. 2202 is a central common chamber. The central common chamber has one or more inlets and one or more outlets, and several tributary channels are connectable by means of respective fluid switches. The fluid switch is connected with each fluid pipeline and/or the negative pressure generating device (which can be a vacuum pump) and/or the atmospheric pressure.
Figure 7C is an isometric view of a central common chamber. The central common chamber may consist of a top, a waist and a bottom, each part carrying a different function. The central common chamber has a bottom and a waist, but may have one or more waists. The central common chamber has a top, a waist, and a bottom. The top of the device is provided with 5 openings, four openings of which are connected with the fluid switch, and one opening of which is connected with the waist. The waist has 6 openings, of which four are connected to the fluid switch, and of which the other two openings can be connected to the top, waist or bottom. The bottom can have 5 openings or 9 openings, wherein 4 openings are necessary for the fluid outlet, and one opening is necessary to be connected with the waist. In this example, 2203 is the top of the central common chamber, 2204 is the waist of the central common chamber, and 2205 is the bottom of the central common chamber. 2206A is a liquid outlet at the bottom 2205 of the central common chamber that is connected to a fluid line leading to a fluid terminal device. The central common chamber top 2205 has two ports for connection to a negative pressure generating device and one may be a liquid overflow port and one needs to be connected to atmosphere. The central common chamber waist 2204 is typically in fluid communication with a fluid line for the liquid. When a certain liquid is required, the fluid switch on the fluid line for this liquid is opened, and at the same time the switch connected to the negative pressure is also opened, so that the liquid enters the central common chamber. If the liquid enters too much, the liquid will flow out of the liquid overflow outlet. When it is desired to deliver liquid from the central common chamber to the fluid end fitting, the switch to the port for atmospheric connection is opened, the pump is started and liquid flows out of the central common chamber 2202.
Figure 7D is a top view of the central common chamber. Figure 7E is a cross-sectional view B-B of the central common chamber. 2207 is a conduit which is part of the top of the central common chamber. One end of the pipe is connected to the top and its length is such that its other end is just below the bottom of the central common chamber. This conduit is connected through the top of the central common chamber to an external gas pressure controller, which may be a vacuum pump. The fluid middle-end device changes the gas pressure in the liquid storage chamber through the pipeline. Meanwhile, the fluid middle-end device can also discharge the liquid in the storage cavity which is used for completing the task out of the storage cavity through the pipeline. When it is desired to drain the liquid from the central common chamber, a negative pressure is created in conduit 2207 and the port to atmosphere is opened and the liquid in the central common chamber can be drained from the fluid center.
Fig. 7F is an isometric view of a fluid tip device. Fig. 7G is a bottom view of the fluid tip device. Fig. 7H is a front view of the fluid tip device. Fig. 7I is a stepped cross-sectional view G-G in an elevation view of the fluid tip assembly. Fig. 7J is a cross-sectional view at the center of the fluid tip device. 2301 is a liquid inlet head of a fluid terminal device, and 2301 is connected to a fluid middle device through a fluid pipeline. When a switch in a fluid pipeline in which a certain liquid exists is opened, the liquid flows into the fluid middle-end device, so that the storage cavity of the fluid middle-end device is filled with the liquid. When the liquid amount in the storage chamber reaches a certain standard, the switch in the fluid pipeline where the liquid exists is closed, and the liquid is stopped from entering. Subsequently, the fluid end device is operated and the liquid in the storage chamber flows out of the outlet of the fluid end device through the fluid line to the fluid end device inlet header 2301 into the fluid end device. The reagent is introduced into the common manifold 2308 and the lower pipe in sequence through the inlet header 2301, and then flows out from the outlet 809 of the lower pipe after a certain amount of liquid is accumulated in the lower pipe to prevent air bubbles. 2302 is the waste outlet head of the fluid end set. The task-performed liquid recovered from the slide eventually exits the fluidic terminal device through a waste outlet header 2302 of the fluidic terminal device. 2303 is a high pressure gas connection of the fluid end unit, through which high pressure gas enters. The conduits of the inlet header 2301 and the conduits of the high pressure gas connection 2303 merge into a common manifold 2308. Attached to the end of the common manifold is a drip needle 2305. In FIG. 7G, 2304 are effluent collecting wells. Waste recovery holes 2304 are distributed in rows on both sides of dropping needles 2305. The number of each row of waste liquid recovery holes is between 5 and 15. The waste liquid recovery hole is connected with the negative pressure chamber. The negative pressure chamber is connected to a negative pressure source through waste outlet header 2302, which can draw negative pressure via tubing to draw fluid out of the negative pressure chamber. When liquid is distributed, the waste liquid recovery hole 2304 and the high-pressure gas connector 2303 are in a non-operating state, and the liquid passes through the liquid inlet head 2301 and the common manifold 2308 and finally goes out of the dropping needle 2305 to reach the slide glass. After the liquid finishes the coloring task, the waste liquid recovery hole 2304 and the high pressure gas joint 2303 start to work. High pressure gas enters the common manifold 2308 from the high pressure gas connection 2303 and then travels in both directions. And upward, to displace liquid in the conduit from the inlet header 2301 out of the fluid end device to the fluid middle device. Flows downward, out of common manifold 2308 to drip needle 2305, and blows liquid onto the slide. The high pressure forces the liquid on the slide to spread from the center to the two sides of the waste liquid recovery hole. At the same time, the low air pressure in the waste liquid recovery hole sucks the liquid into the negative pressure chamber, thereby completing the waste liquid removal work.
When the fluid tip device dispenses reagent liquid onto the surface of the slide 402, the two-dimensional code 403 disposed on the slide 402 can be recognized by the recognition device, processed for marking, and passed information to the human-computer interaction system.
Figure 8A is an isometric view of a portion of an apparatus of a staining module. Fig. 8B is an isometric view of the carrier tray receiving tray and power device. Fig. 8C is a front view of the carrier tray receiving tray and power device. In fig. 8A, 2900 denotes a temperature maintaining device which can radiate or radiate heat to the outside to maintain the temperature inside the dyeing apparatus at a set temperature range or value. 401 denotes a carrier plate carrying slides circumferentially distributed on the carrier plate with their long side axis aligned with the center of the tray. The central region of the carrier plate 401 has a convex spatial geometry with corresponding draft features on the bottom surface of the carrier plate corresponding to these shapes. The fluid end devices 2300A-2300D can precisely align slides carried on carrier trays during the staining process. After one staining process is finished, the carrier tray receiving holder 2401 drives the carrier tray 401 to rotate a certain angle, so that the next slide adjacent to the slide which finishes the staining process can be properly aligned with the fluid end device 2300A-2300D. The carrier plate 401 is combined with the carrier plate receiving tray in a certain manner and can maintain the relative position unchanged. 2401 in fig. 8B shows a carrier tray receiving tray. The carrier tray receiving receptacles 2401 also have raised dimensional geometries that can be precisely matched to the pattern drawing features on the bottom surface of the carrier tray. In one embodiment, the carrier plate 401 has a circular hole 404 that is coupled to a protrusion structure of the carrier plate receiving holder 2401. The power device is provided with a bearing support rotary driving mechanism 2400 to drive the carrier plate bearing support 2401 to rotate. The carrier rotation driving mechanism 2400 may be a motor. The carrier holder rotation driving mechanism 2400 and the carrier tray holder 2401 may be connected to a timing belt pulley by a timing belt. The carrying tray lifting mechanism 2500 and the carrier tray carrying tray 2401 can also be connected through a screw rod, so that the lifting linear motion of the carrier tray carrying tray 2401 is realized.
In fig. 8C, 2400 denotes a carrier tray receiving tray, 2500 denotes a receiving tray lifting mechanism, and 2600 denotes a frame of the dyeing apparatus. The carrier plate receiving tray has at least a lifting linear motion relative to the frame of the dyeing apparatus and a circular motion relative to the frame of the dyeing apparatus. The carrier tray receiving tray has two states of open and working. In the open state, the carrier tray receiving tray does not perform circular motion. In the opening process and the return process, the carrier plate bearing support only moves linearly. In the operating state, the carrier plate carrying holder only performs circular motion. In fig. 6, the carrier tray receiving tray is in an operative state. In the operating state, after each circular motion of the carrier plate receiving tray is stopped, at least one slide glass on the carrier plate receiving tray can be opposite to the fluid end device. Meanwhile, a device is arranged on a rack of the dyeing device to detect whether the position of the carrier plate for bearing the supporting device is accurate or not. The power device provides power for the carrier tray bearing support. In fig. 8C, the carrier tray receiving tray is in an open state.
Automatic drip dyeing mounting equipment with all-in-one machine structure
Referring to fig. 1 to 12, an embodiment of the present invention further provides an automatic dispensing and dyeing mounting apparatus with an all-in-one machine structure, including a frame 700, a carrier tray 401, a carrying and transferring module 500, heat treatment modules 100, 900, an automatic dispensing module 200, and an automatic mounting module 300, where the heat treatment modules 100, 900, the automatic dispensing module 200, and the automatic mounting module 300 are disposed on the frame 700, the carrying and transferring module 500 includes an X-axis guide 503, a Y-axis guide 504, and a carrier tray carrying mechanism, the Y-axis guide 504 is mounted on the frame 700, the X-axis guide 503 is movably mounted on the Y-axis guide 504, the carrier tray carrying mechanism is used for carrying the carrier tray 401, the carrier tray carrying mechanism is movably mounted on the X-axis guide 503, the carrier tray carrying mechanism and the X-axis guide 503 are respectively driven by a driving mechanism (such as a motor), and thus the carrier tray 401 carrying the slide glass is carried to the heat treatment module 100, 900 for dewaxing heat treatment, then carried to the automatic drip-dyeing module 200 for drip-dyeing treatment, then carried to the automatic cover slip module 300 for cover slip mounting treatment, and then carried to the heat treatment module 100, 900 for cover slip reagent heat-curing treatment. The automatic drip dye module 200 may be the automatic drip dye module 200 provided previously herein.
In a preferred embodiment, the rack 700 is a vertical structure, the X-axis guide 503 is disposed along a horizontal direction, the Y-axis guide 504 is disposed along a vertical direction, and the heat treatment modules 100 and 900, the automatic drip-dyeing module 200, and the automatic mounting module 300 are distributed on different height positions of the rack 700 or on different horizontal positions of the same height.
In a preferred embodiment, the heat treatment modules 100 and 900 include first to second heat treatment modules, the automatic drip-dyeing module 200 includes first to third drip-dyeing modules 200, the first heat treatment module 900 is used for dewaxing heating treatment, the second heat treatment module 100 is used for mounting reagent heating and curing treatment, and the first to third drip-dyeing modules 200 can each perform drip-dyeing treatment on one carrier tray 401 at the same time.
In a preferred embodiment, the X-axis guide 503 is slidably mounted on the Y-axis guide 504 via a first slider 505, and the carrier disk carrier mechanism is slidably mounted on the X-axis guide 503 via a second slider 502.
In a preferred embodiment, the automatic drip-dyeing system further comprises a carrier tray temporary storage module 600, wherein the carrier tray temporary storage module 600 is disposed on the rack 700 and is used for receiving and temporarily storing the dewaxed and heat-treated carrier tray 401 carried by the carrier transfer module 500 when the automatic drip-dyeing module 200 has no empty space temporarily.
In a preferred embodiment, the carrier plate carrying mechanism includes a fork-shaped structure 506 extending outward in a cantilever manner, the bottom of the carrier plate 401 is provided with a positioning structure matching with the fork-shaped structure 506, and the carrier plate 401 is carried on the fork-shaped structure 506 through the coupling of the positioning structure 408 and the fork-shaped structure 506. Preferably, the positioning structure 408 protrudes from the bottom surface of the carrier plate 401, and is embedded in the middle of the fork-shaped structure 506 when coupled with the fork-shaped structure 506 so as to position the carrier plate 401. The fork-type structure may be, but is not limited to, a U-shaped structure.
In a preferred embodiment, the rack 700 further comprises a carrier tray access module, the carrier tray access module is a retractable structure 406 disposed at the carrier tray access, the retracted position of the retractable structure is located above the initial position of the carrier tray carrying mechanism, two notches aligned with the extending fingers of the fork-shaped structure 506 are disposed on the retractable structure 406, at least a part of the two notches is covered when the carrier tray 401 is located on the retractable structure 406, and the fork-shaped structure 506 passes through the two notches to transfer the carrier tray 401 from the retractable structure 406 to the carrier tray carrying mechanism when the carrier tray carrying mechanism moves upward; and/or
In a preferred embodiment, the rack 700 further includes a carrier tray receiving module, the carrier tray receiving module is a retractable structure 406 disposed at the carrier tray outlet, the retracted position of the carrier tray receiving module is located below the end position of the carrier tray carrying mechanism, two notches aligned with the extending fingers of the fork-shaped structure 506 are disposed on the retractable structure 406, at least a part of the two notches is covered when the carrier tray 401 is located on the retractable structure 406, and when the carrier tray carrying mechanism moves downward, the fork-shaped structure 506 passes through the two notches to transfer the carrier tray 401 from the carrier tray carrying mechanism to the retractable structure 406.
In a preferred embodiment, the carrier plate 401 is provided with positioning holes 404, and the retractable structure 406 is provided with positioning protrusions 407 for matching with the positioning holes.
In a preferred embodiment, the automatic drip-dyeing module 200 includes a drip-dyeing device, a carrier tray receiving tray for receiving the carrier tray 401 conveyed by the carrying and transferring module 500, a receiving tray lifting mechanism for lifting and moving the carrier tray receiving tray between the receiving position of the carrier tray 401 and a drip-dyeing processing position, and a receiving tray rotation driving mechanism for driving the carrier tray receiving tray to rotate so that the drip-dyeing device can perform drip-dyeing processing on different slides on the carrier tray 401; preferably, the carrier plate 401 is provided with a positioning hole, and the carrier plate receiving holder is provided with a positioning protrusion matched with the positioning hole.
In a preferred embodiment, the heat treatment module 100, 900 includes a heating device for providing a heat source for heat treatment, a carrier tray receiving tray for receiving the carrier tray 401 carried by the carrying and transferring module 500, and a receiving tray lifting mechanism for lifting and moving the carrier tray receiving tray between a receiving position for the carrier tray 401 and a heat treatment position; preferably, the carrier plate 401 is provided with a positioning hole, and the carrier plate receiving holder is provided with a positioning protrusion matched with the positioning hole.
In a preferred embodiment, the automatic drip-dyeing machine further comprises a liquid storage module arranged on the rack 700 and used for supplying liquid to the automatic drip-dyeing module 200 and a waste liquid collecting module used for collecting waste liquid from the automatic drip-dyeing module 200.
Specific embodiments are described further below.
Referring to fig. 1 to 12, in one embodiment, the rack 700, the carrier tray 401, the carrier tray access module 400, the carrying and transferring module 500, the automatic drip dyeing module 200, the liquid storage module 800, the heat treatment modules 100 and 900 (the constant temperature heating and curing system and the drying and dewaxing system), the waste liquid collection module, the automatic mounting module 300, and the human-computer interaction control system are integrated into a whole. The automatic dripping and dyeing treatment system all-in-one machine for the biological tissue sample slices adopts a distributed layout, and can accelerate the treatment efficiency.
Optionally, the automatic drip-staining processing system for biological tissue sample slices further comprises: the device comprises an anti-pollution module and an alarm processing module.
Referring to fig. 1, in a preferred embodiment the housing 700 is vertical. Optionally, the integrated machine of the automatic staining and dripping processing system for biological tissue sample slices can be in a horizontal box type or an off-box type.
Referring to fig. 1, in a preferred embodiment, three drop dyeing modules 200 and two heat treatment modules 100, 900 are provided in order to improve the efficiency of the parallel process according to dyeing time and heat treatment time.
Referring to fig. 2 and 4, in a preferred embodiment, the carrier transfer module 500 includes a two-dimensional guide 504 and moving sliders 505 and 502 to move the carrier plate 401 among the modules of the automatic drip-dyeing module 200, the thermal processing modules 100 and 900, and the automatic cover sealing module 300, and the process thereof includes:
the transport carrier plate 401 moves among the automatic drip dyeing module 200, the heat treatment modules 100 and 900 and the automatic cover sealing module 300;
the circular hole 404 at the bottom of the carrier disc 401 is coupled and fixed with the convex structure 407 of the telescopic structure 406, the telescopic structure 406 is contracted, the motor controls the motion of the motion sliding block 502, so that the fork-shaped structure 506 is coupled and fixed with the U-shaped positioning structure 408 at the bottom of the carrier disc 401, and the motion sliding blocks 505 and 502 are controlled by the motor to drive the carrier disc 401 to move;
conveying the carrier disc 401 into the all-in-one machine from an inlet of the all-in-one machine, and conveying the carrier disc 401 to an outlet of the all-in-one machine from the all-in-one machine;
the automatic drip-dyeing module 200 moves the carried carrier disc 401 into the module for processing, and moves out after processing;
the heat treatment module 100, 900 moves the carried carrier plate 401 into the module for processing, and moves out after processing;
the automated cover module 300 moves the incoming carrier plate 401 into the module for processing and then moves out after processing.
Referring to fig. 3, in a preferred embodiment, a carrier plate 401 may hold slides 402 via removable slots 405, the slides 402 may be removable from the carrier plate 401, and multiple slides 402 may be held on the carrier plate 401 at the same time. The two-dimensional code on the slide 402 can be recognized by the recognition device.
Referring to fig. 3, in a preferred embodiment, the carrier plate 401 is a circular or rectangular symmetrical shape, and the slides 402 are regularly distributed on the carrier plate 401, which is beneficial to streamline processing.
Referring to fig. 5, in a preferred embodiment, a pair of miniature cameras is arranged at the upper part of the all-in-one machine, each module in the all-in-one machine is monitored, images are transmitted to a human-computer interaction interface for display, an infrared scanner scans two-dimensional codes 403 on a glass slide 402 to obtain basic information of a patient and tissue slice processing flow information, data are transmitted to and transmitted into a host, a photosensitive sensor judges whether the glass slide 402 is correctly placed or not according to whether signals are received or not, and the data are transmitted to a human-computer interaction system
Referring to fig. 6-8C, the automatic drip dye module 200 of the preferred embodiment is described above with particular reference thereto.
Referring to fig. 9A-9F, the preferred embodiment thermal processing module 100, 900 is described in detail below.
Heat treatment module and automatic drop dyeing mounting equipment with same
Referring to fig. 9A to 9F, the embodiment of the invention further provides a heat treatment module, which can be used in the aforementioned automatic dispensing and mounting apparatus to perform heat treatment on a slide. The heat treatment module comprises a semi-closed cavity 901, a plurality of heating resistors 907, a blower fan blade 912 and a blower driving mechanism, wherein the heating resistors 907 and the blower fan blade 912 are arranged in the semi-closed cavity 901, the heating resistors 907 are circumferentially distributed in the semi-closed cavity 901, the upper end of the semi-closed cavity 901 is closed and is provided with a bottom opening, a carrier disc 401 carrying a plurality of glass slides can be matched with the semi-closed cavity 901 at the bottom opening to form a closed cavity, the heating resistors 907 heat air in the cavity when being electrified, and the blower driving mechanism is used for driving the blower fan blade 912 to rotate so as to form annular heat convection in the cavity, so that the glass slides on the carrier disc 401 are subjected to uniform convection heating.
In a preferred embodiment, the plurality of heating resistors 907 are uniformly distributed in a ring shape.
In a preferred embodiment, the heat treatment module further comprises a plurality of heating partitions 906 arranged in the semi-closed cavity 901 and vertically distributed in a ring shape, the plurality of heating partitions 906 extend radially outwards around the center of the cavity and are connected to a fixed outer ring, and the plurality of heating resistors 907 are respectively mounted on different heating partitions 906.
In a preferred embodiment, the heat treatment module further comprises a plurality of temperature sensors 908 uniformly arranged in the cavity to generate feedback signals for adjusting the operating states of one or more heating resistors 907 at corresponding positions when the plurality of temperature sensors 908 detect that the temperature in the cavity is not uniform or too high, so as to keep the temperature in the cavity uniform and stable.
In a preferred embodiment, the plurality of temperature sensors 908 are disposed on different heating partitions 906 corresponding to the plurality of heating resistors 907, respectively.
In a preferred embodiment, the thermal treatment module further includes the rotating bracket 911 disposed in the cavity, the rotating bracket 911 includes a rotating shaft 913 and an upper bracket plate and a lower bracket plate coupled to the rotating shaft 913, the blower fan 912 includes a plurality of fan blade units distributed in a vortex shape around a center of the rotating bracket 911, the plurality of fan blade units are vertically fixed between the upper bracket plate and the lower bracket plate, the rotating bracket 911 is disposed on an upper side or a lower side of the plurality of heating partitions 906, or an upper layer and a lower layer of the plurality of heating partitions 906 are disposed in the cavity, and the rotating bracket 911 is disposed between the upper layer of the plurality of heating partitions 906 and the lower layer of the plurality of heating partitions 906.
In a preferred embodiment, the blower driving mechanism comprises a motor 903, a transmission belt 904, and a transmission gear 905, and the motor 903 is coupled to the rotating shaft through the transmission gear 905 and the transmission belt 904.
In a preferred embodiment, the thermal processing module further comprises a carrier tray receiving tray 909 and a receiving tray lifting mechanism 910, wherein the carrier tray receiving tray 909 is configured to receive the transported carrier tray 401, and the receiving tray lifting mechanism 910 is configured to lift and move the carrier tray receiving tray 909 between a receiving position of the carrier tray 401 and the bottom opening of the semi-enclosed cavity 901.
In a preferred embodiment, the carrier plate 401 has a positioning hole, such as a circular hole 404, and the carrier plate receiving holder 909 has a protrusion 914 that matches the positioning hole.
In the preferred embodiment, the semi-enclosed cavity 901 is formed by a cylindrical housing that is open at the bottom and covers the plurality of heating resistors 907 and the blower fan blades.
Specific embodiments are described further below.
Referring to fig. 9A to 9F, in a preferred embodiment, the thermal treatment module 100, 900 includes a semi-enclosed cavity 901, a motor 903, a transmission belt 904, a transmission gear 905, a heating partition 906, a heating resistor 907, a temperature sensor 908, a carrier tray supporting device 909, a thermal treatment module vertical telescopic device 910, a rotating bracket 911, and a blower fan blade 912.
A plurality of layers of heating resistors 907 distributed annularly are arranged in the semi-closed cavity 901 and are connected with the driving circuit integrated module. The semi-enclosed cavity 901 is a cylindrical shell, and the heating part is embedded in the cylindrical shell. Also uniformly disposed within the cylindrical housing are temperature sensors 908 whose values are fed back to the drive circuit integrated module. The heating resistors 907 distributed in a ring shape are matched with the built-in rotating bracket 911, and the rotating bracket 911 is provided with fan blades 912 distributed in a ring-shaped radiation manner. The semi-enclosed cavity 901 may cooperate with the carrier plate 401 to form an enclosed cavity.
The working mode is as follows: the heating resistor 907 works, the motor 903 drives the transmission belt 904 to drive the rotating support 911 to rotate, so that the fan blades 912 of the air blower are driven to rotate, annular heat convection is formed, and the temperature in the semi-closed cavity is uniformly heated and increased. The temperature sensor 908 feeds back the temperature to the driver circuit integrated module, and for the part with uneven temperature or too high temperature, the heating resistor 907 is adjusted to finally keep the heating temperature distribution uniform and stable.
The carrier plate 401 may be carried to the heat treatment module 100, 900 by the carrier transfer module. Under the transportation of the carrier tray 401 by the carrying and transferring module 500, the circular hole 404 at the bottom of the carrier tray 401 is coupled and fixed with the protrusion structure 914 of the carrier tray receiving holder 909 in the heat treatment module 100, 900, then the receiving holder lifting mechanism 910 of the heat treatment module is lifted to lift the carrier tray receiving holder 909 carrying tray 401, the carrier tray 401 is matched with the bottom opening of the semi-closed cavity 901 to form a closed cavity, the carrier tray 401 carries the slide glass 402, and the slide glass 402 is heated and processed in the closed cavity for a certain time by annular heat convection. Before the slide 402 is drip stained, the thermal processing module 900 is used for carrying out the convection baking dewaxing treatment at 140 ℃ for 4 minutes, after the slide 402 is dewaxed, the slide 402 is drip stained and mounted, and then the slide is conveyed to the thermal processing module 100 to be carried out the mounting curing treatment at 70 ℃ for 5 minutes.
In the convection baking process, the paraffin covering on the surface of the biological tissue sample of the slide 402 on the carrier plate 401 is heated to a liquid state and dropped under the action of gravity. After the slide 402 is drop-stained by the automatic drop staining module, a coverslipping reagent is added to the surface of the slide 402 by the automatic coverslipping module, and the slide is covered. In the curing treatment, the mounting reagent can be rapidly cured by heating treatment at 70 ℃ for 5 minutes. The mounting agent is a liquid agent such as cyanoacrylate adhesive or a photocurable adhesive polymer.
Human-computer interaction module and automatic drop dyeing mounting equipment with same
Referring to fig. 1A to 1C and fig. 10 to 12, an embodiment of the present invention further provides an automatic dispensing and sealing apparatus with a human-computer interaction function, including a rack, a carrier tray, a carrying and transferring module, a heat treatment module, an automatic dispensing module, an automatic sealing module, a human-computer interaction module, and a control unit, where the carrying and transferring module is configured to transport the carrier tray on the rack, the heat treatment module, the automatic dispensing module, and the automatic sealing module are disposed on a carrier tray transport route on the rack, the carrier tray carries a slide, the heat treatment module is configured to perform dewaxing heating processing and sealing reagent heating and curing processing on the slide, the automatic dispensing module is configured to perform dispensing processing on the slide, the automatic sealing module is configured to perform cover glass sealing processing on the slide, and the control unit controls operation processes and processing processes of the modules, the human-computer interaction module comprises a human-computer interaction interface, and is connected to the control unit and used for controlling the input of instructions and the output of processing information.
In a preferred embodiment, the automated drop-dye mounting apparatus further comprises a surveillance recognition system comprising one or more of: the camera is arranged on the rack and used for monitoring each module, and monitoring images are transmitted to the human-computer interaction interface for display; the infrared scanner is arranged on the rack and used for scanning the two-dimensional code on the glass slide to acquire sample information and processing flow information of a tissue section corresponding to the glass slide and transmitting the acquired information to the control unit, and the control unit controls the operation flow and the processing process of each module according to the acquired information; and the photosensitive sensor is arranged on the rack and used for detecting whether the glass slide is correctly placed or not, transmitting a detection signal to the control unit and processing or giving an alarm by the control unit.
Specific embodiments are described further below.
The human-computer interaction module comprises a human-computer interaction interface, interacts with a control host, adjusts the operation flow and the processing process of the bio-tissue sample section staining mounting by inputting a control instruction to the microprocessor, and comprises the steps of moving a two-dimensional platform, stopping, moving and closing liquid drops, adding a liquid drop reagent sequence, finely adjusting a micro two-dimensional sliding table, monitoring and identifying signals, operating and designing the processing of different bio-tissue sample sections through the human-computer interaction interface, carrying out directional batch processing on various bio-tissue sample sections, and selecting a staining flow according to the requirements through a human-computer interaction system, thereby achieving the effects of accuracy, high efficiency, no pollution and reagent saving. The man-machine interaction interface comprises a display and an input device, and is used for controlling the input of instructions and the output of information. A pair of miniature cameras are arranged on the upper portion of a rack of the all-in-one machine and used for monitoring all modules in the all-in-one machine, and images are transmitted to a human-computer interaction interface to be displayed. The infrared scanner is used for scanning the two-dimensional code on the glass slide to obtain the sample information (such as basic information of a patient) of the tissue section and the processing flow information of the tissue section, transmitting the data to the host, judging whether the glass slide is correctly placed or not by judging whether the signal is received or not by the photosensitive sensor, and transmitting the data to the man-machine interaction system.
The automated drop-dye mounting apparatus of various preferred embodiments is described in detail above.
The background of the present invention may contain background information related to the problem or environment of the present invention and does not necessarily describe the prior art. Accordingly, the inclusion in the background section is not an admission of prior art by the applicant.
The foregoing is a more detailed description of the invention in connection with specific/preferred embodiments and is not intended to limit the practice of the invention to those descriptions. It will be apparent to those skilled in the art that various substitutions and modifications can be made to the described embodiments without departing from the spirit of the invention, and these substitutions and modifications should be considered to fall within the scope of the invention. In the description herein, references to the description of the term "one embodiment," "some embodiments," "preferred embodiments," "an example," "a specific example," or "some examples" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction. Although embodiments of the present invention and their advantages have been described in detail, it should be understood that various changes, substitutions and alterations can be made herein without departing from the scope of the claims.
Claims (10)
1. An automatic drip-dyeing mounting device comprises an automatic drip-dyeing module for automatically dyeing a biological tissue sample slide glass and an automatic mounting module for mounting the dyed slide glass, it is characterized in that the automatic drop dyeing module comprises a fluid middle-end device, a fluid tail-end device, a carrier disc and a carrier disc bearing support tool, the carrier plate is used for bearing glass slides and is supported and kept by the carrier plate supporting and holding device, the fluid middle-end device is connected with the fluid end device through a fluid pipeline, the fluid middle-end device distributes externally input dyeing liquid to the fluid end device, the fluid end device is provided with a liquid outlet and a waste liquid recovery hole, the dyeing liquid is applied to the glass slide carried by the carrier disc from the liquid outlet, and after the sample on the glass slide is dyed, the sample is recovered to a negative pressure chamber through the waste liquid recovery hole in a negative pressure suction mode.
2. The automated drip dye mounting apparatus of claim 1, further comprising a power device including a take-up tray rotary drive mechanism coupled to the carrier tray take-up tray for driving the carrier tray take-up tray to rotate in a work position to position a slide to be dyed relative to the fluid end device and a take-up tray lift mechanism for moving the carrier tray take-up tray up and down between a take-up position of a carrier tray and a drip dye processing position.
3. An automated drop dyeing mounting apparatus according to claim 1 or 2, wherein said fluid terminal means comprises a liquid inlet head connected to said liquid outlet via a common manifold, said liquid inlet head being connected to said fluid terminal means via a fluid line, and a high pressure gas connection for introducing high pressure gas during waste liquid recovery, a plurality of said waste liquid recovery holes being distributed around said liquid outlet.
4. The automated drop dye mounting apparatus of any one of claims 1 to 3, further comprising a pump between the fluidic middle end device and the fluidic end device.
5. The automated drop dye mounting apparatus of any one of claims 1 to 4, wherein the fluid-middle-end device comprises a liquid storage chamber provided with a fluid inlet for inputting a dyeing liquid, a fluid outlet for dispensing a liquid to the fluid-end device, and at least one of: a negative pressure suction port for forming a negative pressure in the liquid storage chamber to input a staining liquid, a liquid overflow port for overflowing the liquid when the liquid input is excessive, an atmospheric connection port for communicating with the atmosphere when the liquid is dispensed to the fluid end device, and a discharge port for discharging the liquid after the staining of the sample is finished; preferably, the liquid storage chamber is provided with a plurality of fluid inlets; preferably, the fluid inlet is provided with a fluid switch; preferably, the negative pressure suction port and the discharge port are the same port.
6. The automated dip dyeing mounting apparatus according to claim 5, wherein said fluid middle-end device comprises a combination of a top portion, at least one waist portion and a bottom portion stacked together in a square shape, said liquid storage chamber is formed by said combination, a plurality of side surfaces of said top portion and a bottom surface connected to said waist portion are respectively provided with openings, a plurality of side surfaces of said at least one waist portion are respectively provided with openings, and a top surface of said waist portion connected to said top portion is provided with an opening, a bottom surface of said waist portion connected to said bottom portion is provided with an opening, a plurality of side surfaces of said bottom portion and a top surface connected to said waist portion are respectively provided with openings, an opening of said top portion is used for liquid or air, and openings of said waist portion and said bottom portion are used for liquid.
7. The automated drop dye mounting apparatus of any one of claims 5 to 6 wherein the fluid middle-end device is provided with a negative pressure conduit connected to the negative pressure suction port and extending to the floor of the liquid storage chamber.
8. The automatic dispensing and mounting apparatus according to any one of claims 1 to 7, further comprising a heat treatment module for performing heat treatment on the glass slides, wherein the heat treatment module comprises a semi-closed cavity, a plurality of heating resistors, a blower fan blade and a blower driving mechanism, the heating resistors and the blower fan blade are disposed in the semi-closed cavity, the heating resistors are circumferentially distributed in the semi-closed cavity, the upper end of the semi-closed cavity is closed and has a bottom opening, a carrier tray carrying the glass slides can be engaged with the semi-closed cavity at the bottom opening to form a closed cavity, the heating resistors heat air in the cavity when being powered on, the blower driving mechanism is used for driving the blower fan blade to rotate to form annular thermal convection in the cavity, thereby providing uniform convective heating of the plurality of slides on the carrier tray; preferably, the temperature control device further comprises a plurality of temperature sensors uniformly arranged in the cavity, so that when the plurality of temperature sensors detect that the temperature in the cavity is not uniform or too high, a feedback signal for adjusting the working state of one or more heating resistors at corresponding positions is generated, and the temperature in the cavity is kept uniform and stable; preferably, the plurality of temperature sensors are respectively disposed on different heating partitions corresponding to the plurality of heating resistors.
9. The automated drop-dyeing mounting apparatus according to claim 8, further comprising a plurality of heating partitions disposed in the semi-enclosed cavity and vertically distributed in a ring shape, wherein the plurality of heating partitions radially extend outward around the center of the cavity and are connected to a fixed outer ring, and the plurality of heating resistors are respectively mounted on different heating partitions.
10. The automated drop dye mounting apparatus according to any one of claims 8 to 9, further comprising the rotary support disposed in the cavity, wherein the rotary support comprises a rotary shaft and an upper support plate and a lower support plate coupled to the rotary shaft, wherein the blower fan comprises a plurality of fan blade units distributed in a vortex shape around a center of the rotary support, the plurality of fan blade units are vertically fixed between the upper support plate and the lower support plate, the rotary support is disposed on an upper side or a lower side of the plurality of heating partition plates, or an upper layer and a lower layer of the plurality of heating partition plates are disposed in the cavity, and the rotary support is disposed between the plurality of heating partition plates on the upper layer and the plurality of heating partition plates on the lower layer.
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| CN202011601497.4A CN112730000B (en) | 2020-12-29 | 2020-12-29 | Automatic change drop dyeing sealing piece equipment |
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| CN113790940A (en) * | 2021-09-08 | 2021-12-14 | 深圳市森盈生物科技有限公司 | Serial-type waste liquid is clear away and automatic temperature control system |
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