CN112730000B - Automatic change drop dyeing sealing piece equipment - Google Patents
Automatic change drop dyeing sealing piece equipment Download PDFInfo
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- CN112730000B CN112730000B CN202011601497.4A CN202011601497A CN112730000B CN 112730000 B CN112730000 B CN 112730000B CN 202011601497 A CN202011601497 A CN 202011601497A CN 112730000 B CN112730000 B CN 112730000B
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Abstract
The automatic drop dyeing and sealing piece equipment comprises an automatic drop dyeing module and an automatic sealing piece module, wherein the automatic drop dyeing module comprises a fluid middle end device, a fluid end device, a carrier disc and a carrier disc bearing support, the carrier disc is used for bearing a glass slide, the carrier disc is borne and held by the carrier disc bearing support, the fluid middle end device is connected with the fluid end device through a fluid pipeline, the fluid middle end device distributes externally input dyeing liquid to the fluid end device, the fluid end device is provided with a liquid outlet and a waste liquid recovery hole, the dyeing liquid is applied to the glass slide borne by the carrier disc through the liquid outlet, and a negative pressure cavity is received through waste liquid recovery Kong Hui in a negative pressure suction mode after dyeing of a sample on the glass slide is finished. The automatic drop dyeing module improves the dyeing treatment efficiency of the samples, reduces labor intensity, and simultaneously reduces the possibility of cross contamination among the samples, so that the sample treatment process has consistency and controllability.
Description
Technical Field
The invention relates to an automatic drip-dyeing sealing piece technology for biological tissue sample slices, in particular to an automatic drip-dyeing sealing piece device.
Background
The automatic dyeing treatment technology for the biological tissue sample slice is an important step and an indispensable step in the whole automatic dyeing, drying and sealing process of the biological tissue sample, so that the treated biological tissue sample has a quality and a phase which are convenient to observe, the consistency and the high efficiency of the treatment process can be maintained, and the dyeing step is an object which is required to be strictly controlled in the treatment process of the biological tissue sample.
The purpose of staining is to make different structures within the tissue appear different colors for ease of viewing. Classical Hematoxylin (Hematoxylin) and Eosin staining methods are conventional staining of histological specimens and pathological section specimens, abbreviated as HE staining. After staining, the nuclei were stained with hematoxylin to purplish blue and most cytoplasmic and non-cellular components were stained with eosin to pink.
Various techniques may be used to analyze biological samples. Examples of analytical techniques include microscopic analysis, microarray analysis (e.g., protein and nucleic acid microarray analysis), and mass spectrometry. Preparing samples for these and other types of analysis typically includes: the sample is contacted with a series of treatment fluids. Some of these treatment fluids (e.g., staining reagents and counterstaining reagents) may add color and control or otherwise alter the visual characteristics of invisible or difficult to see sample components (e.g., at least some types of cells and intracellular structures). Other treatment liquids (e.g., deparaffinization liquids) may be used to achieve other treatment objectives. If multiple processing liquids are used to process the sample, the application and subsequent removal of the various processing liquids can be important to produce a sample suitable for analysis. In some cases, treating the sample with the plurality of treatment fluids includes: the treatment liquid is manually applied to the microscope slides that each carry a sample. This method of processing samples tends to be labor intensive and inaccurate.
The dyeing process of the pathological sections by manpower has the characteristics of large time consumption, easy error, difficult batch processing and the like, and the pathological section samples can be automatically processed in batches by using an automatic dyeing machine.
The existing immersion type automatic dyeing machine can replace manual dyeing. These machines automatically process samples by immersing a rack carrying microscope slides in an open bath of processing liquid. Unfortunately, operation of the immersion method machine inevitably results in movement of the rack carrying the microscope slides from one bath to another, which can result in the intersection of liquids from the different baths. Over time, this movement causes degradation of the treatment fluid. Moreover, when the samples are immersed in a common bath, there is a possibility of cross-contamination. For example, cells may leave a sample on one slide and be transported to another slide in a common bath. This form of contamination greatly reduces the accuracy of certain types of sample analysis. To alleviate this problem and to address degradation of the treatment liquid by movement, it is often necessary to frequently replace the bath of treatment liquid in the immersion process machine. As a result, these machines tend to consume relatively large volumes of treatment liquid, which increases the costs associated with operating these machines. Open baths of these treatment liquids are prone to evaporation loss and oxidative degradation of some of the treatment liquid components. For example, oxidation of certain components of the staining reagent may alter the staining properties of those components and may result in reduced precision of the staining operation.
Most automatic dyeing machines are large in size, sample glass slides are fixed, all modules are mechanically operated alternately corresponding to the same number of three-dimensional movements, mechanical control is complex, liquid reagent types cannot be excessive, or alternative spray heads are adopted, but cleaning is time-consuming and liquid drops remain in the previous operation. The human-computer interaction is poor, biological tissue slices can be processed only according to a fixed mode, the modes such as accurate or efficient setting or batch setting cannot be selected according to requirements, different dyeing programs cannot be selected corresponding to different tissue materials, and real-time supervision and feedback in the dyeing process are absent.
When the automatic drop dyeing and sealing piece treatment is carried out, how to heat a plurality of glass slides on a carrier tray at the same time and at a uniform and stable temperature is a problem to be solved.
It should be noted that the information disclosed in the above background section is only for understanding the background of the present application and thus may include information that does not form the prior art that is already known to those of ordinary skill in the art.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provides automatic drop dyeing sealing equipment.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
an automatic drip-dyeing and sealing device comprises an automatic drip-dyeing module for automatically dyeing biological tissue sample glass slides and an automatic sealing module for sealing the dyed glass slides, wherein the automatic drip-dyeing module comprises a fluid middle end device, a fluid end device, a carrier disc and a carrier disc bearing carrier, the carrier disc is used for bearing the glass slides, the carrier disc is borne and held by the carrier disc bearing carrier, the fluid middle end device is connected with the fluid end device through a fluid pipeline, the fluid middle end device distributes externally input dyeing liquid to the fluid end device, a liquid outlet and a waste liquid recovery hole are formed in the fluid end device, the dyeing liquid is applied to the glass slides borne by the carrier disc through the liquid outlet, and the dyeing liquid is recovered to a negative pressure cavity through the waste liquid recovery hole in a negative pressure suction mode after dyeing of the sample on the glass slides is finished.
In some embodiments, the automatic drop dyeing module further comprises a temperature maintaining device for maintaining the temperature within the dyeing device at a set temperature range or temperature value.
In some embodiments, an automated apparatus for dispensing a liquid onto biological tissue samples on one or more microscope slides, which may perform processing operations on the slides carrying the biological tissue samples, to effect fully automated staining of the biological tissue samples, the apparatus comprising: a fluid mid-end device comprising a central common chamber; a fluid end device comprising a fluid end head assembly; a carrier tray receiving tray comprising a rack; a carrier tray rotation drive mechanism including a motor coupled to the carrier tray and configured to rotate the carrier tray to position slides relative to the fluid end device; a fluid line, a fluid switch, a pump, and a temperature maintaining device may also be included.
Some embodiments may store liquid from a liquid source where the storage is not long term storage. When the liquid is stored, a negative pressure state is formed in the central public chamber, and the liquid in the pipeline is forced to flow into the central public chamber under the action of atmospheric pressure.
Some embodiments may dispense the stored liquid. When the liquid is dispensed, the liquid in the central common chamber can be pumped away under the action of the pump.
Some embodiments can process a slide-borne sample. The treatment process comprises the following steps: liquid pumped from the central common chamber is delivered to the slide by the pump. The liquid coming out will form a concentrated liquid due to the surface tension. The accumulation liquid has a shape that is at least partially maintained and completely covers the sample due to the surface tension. The liquid is then removed from the sample, and the volume of liquid remaining on the slide is at least ten percent less than the previously mentioned volume of aggregate liquid, to expose the sample for cleaning and next staining. The above operation may be repeated and the applied liquid may be of different kinds.
Some embodiments control the pump's operating power during application of the liquid so that the liquid does not splash when it reaches the slide after exiting the fluid end device.
In some embodiments, the liquid remaining in the central common chamber may be recovered. The recovery method is as follows: the central public chamber is internally provided with a pipe which is communicated with the bottom of the chamber, negative pressure is formed in the pipe, and the air pressure in the central public chamber is kept balanced with the atmospheric pressure. The liquid remaining in the central common chamber is forced into the conduit by the air pressure and exits the central common chamber.
A method of processing a sample on an upper surface of a microscope slide comprising: the microscope slide is moved to a processing position. Moving the microscope slide to the processing position refers to receiving a tray carrying the microscope slide from the outside and moving the tray to a position where the slide carried on the tray can be directed against the fluid end device. By facing here is meant that the position of the sample on the slide is just below the fluid outlet, with a position error within a radius of 3 mm. The central axis of the slide in the longer length direction is toward the center of the carrier tray. The shorter length direction of the slide coincides with the width direction of the fluid end device. After sample processing on the previous microscope slide is completed, the next microscope slide can reach the position.
A method of processing a sample on a microscope slide comprising: the reagent is dispensed from an outlet of the fluid dispensing mechanism opposite the upper surface of the microscope slide, forming a layer of reagent in contact with the sample at the mounting area.
In some embodiments, the staining apparatus includes one or more fluid lines and a fluid end device that is fixed. The fluid end device may be coupled to the fluid mid-end device and configured to dispense reagent from one or all of the fluid mid-end devices. At the same time, the fluid end device has both liquid removal and aspiration functions. In some embodiments, the fluid end device may be blown to facilitate movement of liquid to the low pressure region. In some embodiments, the fluid end device comprises: a fluid inlet, a waste outlet, a waste recovery orifice, a gas inlet, and a common manifold. Fluid may be delivered through a manifold and dispensed from the head of a fluid end device.
In some embodiments, the staining apparatus is configured to receive a microscope carrier tray carrying microscope slides from the conveyor apparatus. The stainer module includes: a microscope carrier tray holder, a rotating device and a linear motion device. The microscope carrier disk retainer can drive the microscope carrier disk to rotate, and can control the rotating angle and precision. The control of the position accuracy of the microscope carrier tray on the transport mechanism is already achieved, and when the staining starts from the reception of the microscope carrier tray by the conveyor device, the control of the position accuracy is transferred to the staining device.
In some embodiments, a method of processing a sample on a microscope slide includes: the liquid is applied to the slide and high pressure gas is directed toward the upper surface of the slide to spread the applied liquid toward the two weeks of the slide.
In some embodiments, a method of processing a sample on a microscope slide includes: the whole dyeing device can control the temperature within a certain range, so that the temperature of the sample is kept approximately constant during the dyeing process. The temperature is controlled by forming a constant temperature steady flow of gas within the apparatus, particularly on the microscope carrier tray, by means of a heating device and a fan.
In some embodiments, a method of processing a sample on a microscope slide includes: the presence of a slide beneath the fluid end device is checked and can be detected by a scanner. If there is no slide, then no staining operation is performed.
In a particular embodiment, a method of processing a sample on a microscope slide includes: slides are transported into the stainer module. A liquid is applied to the slide to bring the sample into contact with the liquid. The liquid is blown along and removed from the upper surface of the slide. The slide may then be removed from the stainer module.
The invention has the following beneficial effects:
the invention provides an automatic drip-dyeing sealing piece device for biological tissue sample slices, which can carry out drip-dyeing sealing piece processing operation on a glass slide bearing biological tissue samples, wherein an automatic drip-dyeing module improves the dyeing processing efficiency of the samples, reduces labor intensity, effectively reduces the possibility of cross contamination among the samples, and ensures that the sample processing process has consistency and controllability. The invention is beneficial to realizing the automatic dyeing treatment of the full-automatic biological tissue samples in batches, high efficiency, simplicity and accuracy.
In a preferred scheme, the invention provides a heat treatment module for an automatic drop dyeing and sealing device, which is characterized in that a semi-closed cavity which can jointly form a closed cavity after being matched with a carrier tray for carrying a plurality of glass slides is arranged, a plurality of heating resistors and blower blades are circumferentially distributed in the cavity, and the blower blades are driven to rotate to form annular heat convection in the cavity while heating air in the cavity, so that the plurality of glass slides on the carrier tray are heated by uniform convection.
In the preferred scheme, the automatic drop dyeing and sealing piece equipment has an integrated machine structure, wherein a heat treatment module, an automatic drop dyeing module and an automatic sealing piece module are arranged on a rack in a distributed layout, a carrying and transferring module of an X-axis guide rail and a Y-axis guide rail is arranged on the rack, and is matched with the space operation mode of each module distributed on the rack, and a carrying and transferring module carries a carrying disc to each module for automatic flow treatment, so that the drop dyeing and sealing piece treatment efficiency is effectively improved, and the volume and occupation of the whole set of process treatment equipment are also remarkably reduced. The relative motion mechanism of moving the sample slide between the processing modules by the carrying and transferring module on the rack also reduces the complex mechanical alternating operation caused by the slide transfer between the modules, and effectively reduces the complexity of mechanical control. The automatic distributed assembly line is realized on the structure of the integrated machine, so that the effects of directional and batch treatment, accuracy, high efficiency, no pollution and reagent saving are achieved, and the customization and adjustment of the manual directional treatment route can be realized simply and conveniently as required.
The invention constructs an automatic processing system for executing one or more slide processing operations on the slide glass carrying the biological specimen, and can perform directional batch processing on various biological tissue sample slices, thereby achieving the effects of accuracy, high efficiency, no pollution and reagent saving. The system may provide high specimen throughput while also maximizing the likelihood of reducing or limiting slide cross-contamination.
The automatic drop dyeing and sealing device can also provide a man-machine interaction function, realize a self-defined processing mode, and can monitor and feed back when processing tissue slices.
Drawings
FIG. 1A is a front view of the architecture of an integrated machine for an automated drop-dyeing processing system for biological tissue sample slices in accordance with one embodiment of the present invention;
FIG. 1B is a left side view of an integrated machine configuration for an automated drop-dyeing processing system for biological tissue sample slices in accordance with one embodiment of the present invention;
FIG. 1C is a top view of an integrated machine for an automated drop-dyeing processing system for biological tissue sample slices, in accordance with one embodiment of the present invention;
FIG. 2A is a block diagram of a carrier transport module according to one embodiment of the present invention;
FIG. 2B is a bottom view of a carrier transfer module in accordance with one embodiment of the present invention;
FIG. 2C is a top view of a carrier transport module according to one embodiment of the invention;
FIG. 2D is a schematic diagram of a method for transporting a carrier tray of a carrier transport module according to an embodiment of the invention;
FIG. 3A is a top view of a carrier platter according to an embodiment of the invention;
FIG. 3B is a bottom view of a carrier tray according to one embodiment of the invention;
FIG. 4 is a block diagram of an inlet carrier transport module according to one embodiment of the present invention;
fig. 5 is a block diagram of a portion of a man-machine interaction module according to an embodiment of the present invention:
fig. 6 is a perspective view of an automatic drop dyeing module according to an embodiment of the present invention.
Fig. 7A is an isometric view of a mid-fluid end device of one embodiment of the invention.
Fig. 7B is a top view of a mid-fluid end device according to one embodiment of the invention.
Figure 7C is an isometric view of a central common chamber of an embodiment of the invention.
Fig. 7D is a top view of a central common chamber of an embodiment of the present invention.
FIG. 7E is a B-B cross-sectional view of a central common chamber of an embodiment of the present invention.
Fig. 7F is an isometric view of a fluid end device according to one embodiment of the invention.
Fig. 7G is a bottom view of a fluid end device according to one embodiment of the invention.
Fig. 7H is a front view of a fluid end device according to one embodiment of the invention.
FIG. 7I is a stepped cross-sectional view of G-G in a front view of a fluid end device according to one embodiment of the present invention.
Fig. 7J is a cross-sectional view at the center of a fluid end device according to one embodiment of the invention.
Figure 8A is an isometric view of a portion of a staining module apparatus of one embodiment of the invention.
Figure 8B is an isometric view of a carrier tray receiving tray and power device according to one embodiment of the invention.
Fig. 8C is a front view of a carrier tray receiving tray and power unit according to one embodiment of the invention.
FIG. 9A is a block diagram of a thermal processing module according to one embodiment of the invention;
FIG. 9B is a front view of a thermal processing module according to one embodiment of the invention;
FIG. 9C is a diagram illustrating the internal configuration of a half-cavity of a thermal processing module according to one embodiment of the present invention;
FIG. 9D is a diagram of the heating portion of a thermal processing module according to one embodiment of the present invention;
FIG. 9E is a block diagram of a heating portion of a thermal processing module according to one embodiment of the present invention;
FIG. 9F is a schematic diagram illustrating the operation of the carrier in the thermal processing module according to one embodiment of the present invention;
FIG. 10 is a block diagram of the overall architecture of an automated drip-dyeing processing system for biological tissue sample slices in accordance with one embodiment of the present invention;
FIG. 11 is a block diagram illustrating a man-machine interaction control system according to an embodiment of the present invention;
FIG. 12 is a schematic diagram of an automated drop-dyeing processing system for human-machine interaction control of biological tissue sample slices in accordance with one embodiment of the present invention.
Detailed Description
The following describes embodiments of the present invention in detail. It should be emphasized that the following description is merely exemplary in nature and is in no way intended to limit the scope of the invention or its applications.
It will be understood that when an element is referred to as being "mounted" or "disposed" on another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element. In addition, the connection may be for both a fixing action and a coupling or communication action.
It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are merely for convenience in describing embodiments of the invention and to simplify the description by referring to the figures, rather than to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the embodiments of the present invention, the meaning of "plurality" is two or more, unless explicitly defined otherwise.
Automatic drop dyeing module and automatic drop dyeing and sealing piece equipment with same
Referring to fig. 6-8C, in one embodiment, an automated drop-dyeing and sealing apparatus includes an automatic drop-dyeing module for automatically dyeing biological tissue sample slides and an automatic sealing module for sealing the dyed slides, the automatic drop-dyeing module including a fluid end device 2200, fluid end devices 2300A-2300D, a carrier tray 401 and a carrier tray 2401, the carrier tray 401 for carrying slides, the carrier tray 401 being held by the carrier tray 2401, the fluid end device 2200 being connected to the fluid end devices 2300A-2300D by a fluid line, the fluid end device 2200 acting as a fluid diverter for distributing externally input dyeing fluid to the fluid end devices 2300A-2300D, the fluid end devices 2300A-D having a fluid outlet and a waste fluid recycling hole 2304, the dyeing fluid being applied to the slides carried by the carrier tray 401 via the fluid outlet 2300D, and being recycled by suction of the negative pressure on the slides 2304 after the dyeing is completed (no negative pressure is applied to the chamber).
In a preferred embodiment, a power unit is also included, the power unit including a tray rotation drive mechanism 2400 and a tray lift mechanism 2500, the tray rotation drive mechanism 2400 coupled to the tray 2401 for driving the tray 2401 to rotate in an operative position to position slides to be stained relative to the fluid end devices 2300A-2300D; preferably, the tray lifting mechanism 2500 is further configured to lift and move the tray support tray 2401 between a support position for the tray and a drip dyeing treatment position.
In a preferred embodiment, the fluid end devices 2300A-2300D include a fluid inlet head 2301 connected to the fluid outlet via a common manifold 2308 and a high pressure gas fitting 2303, the fluid inlet head 2301 connected to the fluid mid-end device 2200 via a fluid line, the high pressure gas fitting 2303 for receiving high pressure gas during waste fluid recovery, and a plurality of waste fluid recovery holes 2304 distributed around the fluid outlet. The high pressure gas forces liquid on the slide from the outlet toward the waste recovery well 2304 and forces liquid in the tubing from the inlet head 2301 out of the fluid end devices 2300A-2300D back to the fluid mid-end device 2200.
In a preferred embodiment, the liquid outlet comprises a drip needle 2305, and a plurality of the waste liquid recovery holes 2304 are distributed in rows on both sides of the drip needle 2305.
In a preferred embodiment, pumps 2800A-2800D are also included between the fluid mid-end device 2200 and the fluid end devices 2300A-2300D to power the liquid.
In a preferred embodiment, the fluid mid-end device 2200 includes a liquid storage chamber (e.g., a central common chamber 2202) that is provided with a fluid inlet for inputting a dyeing liquid, a fluid outlet for dispensing liquid to the fluid end devices 2300A-2300D, and at least one of: a negative pressure suction port for forming a negative pressure in the liquid storage chamber for inputting a dyeing liquid, a liquid overflow port for overflowing the liquid when the liquid is input excessively, an atmosphere connection port for communicating with the atmosphere when the liquid is dispensed to the fluid end devices 2300A to 2300D, and a discharge port for discharging the liquid after the dyeing of the sample is completed, preferably the negative pressure suction port is the discharge port.
In a preferred embodiment, the liquid storage chamber is provided with a plurality of fluid inlets.
In a preferred embodiment, one or more of said fluid inlets are provided with a fluid switch.
In a preferred embodiment, the fluid middle-end device 2200 comprises a combination of a top 2203, at least one waist 2204 and a bottom 2205 stacked together in a square shape, wherein the fluid storage chamber is formed by the combination, the sides of the top 2203 and the bottom surface connected to the waist 2204 are respectively provided with openings, the sides of the at least one waist 2204 are respectively provided with openings, the top surface of the waist 2204 connected to the top 2203 is provided with openings, the bottom surface of the waist 2204 connected to the bottom 2205 is provided with openings, i.e. fluid outlets 2206A, the sides of the bottom 2205 and the top surface connected to the waist 2204 are respectively provided with openings, the openings of the top 2203 are used for fluid or air communication, and the openings of the waist 2204 and the bottom 2205 are used for fluid communication.
In a preferred embodiment, the fluid mid-end device 2200 is provided with a negative pressure conduit 2207, the negative pressure conduit 2207 being connected to the negative pressure suction port and extending to the bottom of the liquid storage chamber.
In a preferred embodiment, a temperature maintaining device 2900 is also included for maintaining the temperature within the dyeing device at a set temperature range or temperature value.
Specific embodiments are described in further detail below.
An automatic drip-staining module for a biological tissue sample slice comprising: fluid lines (not shown), fluid mid-end device 2200, fluid end devices 2300A-2300D, fluid switches, carrier tray receptacle 2401, receptacle rotational drive mechanism 2400, receptacle lift mechanism 2500, temperature maintenance device 2900, pumps 2800A-2800E, and rack 2600. A fluid line connects an external liquid source (e.g., a liquid storage device) with the fluid mid-end device 2200, the fluid line connects the fluid mid-end device 2200 with the fluid end devices 2300A-2300D, and pumps 2800A-2800D are included between the fluid mid-end device 2200 and the fluid end devices 2300A-2300D. The fluid line includes a liquid conduit (preferably a flexible, corrosion resistant conduit), a fluid switch, and a mouthpiece. The fluid mid-end device 2200 includes a fluid switch, a central common chamber, and an interface. The mid-fluid end device 2200 may store and dispense liquids. The fluid end devices 2300A-2300D include a plurality of interfaces, waste recovery holes 2304, and a plurality of manifolds to enable application of liquid, waste recovery, and air blowing operations. The carrier tray receiving tray 2401 may drive the carrier tray to rotate and translate. The temperature maintaining device can maintain the temperature in the dyeing device to be approximately constant, so as to realize the optimal dyeing effect. The frame holds the various sections.
The main dyeing process is as follows: the mid-fluid end device 2200 distributes externally inflowing liquid to the fluid end devices 2300A-2300D. Liquid is delivered by fluid end devices 2300A-2300D to slides of carrier tray 401 held by carrier tray receptacle 2401. After the task of this liquid is completed, this liquid is in turn recovered by the fluid end devices 2300A-2300D. The positions of the fluid lines, the fluid mid-end device 2200, and the fluid end devices 2300A-2300D are typically maintained stationary relative to the ground, and the carrier tray receptacle 2401 rotates the carrier tray 401 to effect the staining of specimens on each slide one by one. The carrier tray receiving tray 2401 may receive the carrier tray 401 when open and be in an operative state when closed. The temperature maintaining means maintains the temperature in the dyeing apparatus substantially constant.
In fig. 6, 2200 represents a fluid mid-end device and 2300A-2300D fluid end devices. The dyeing module can have a plurality of fluid pipelines, and each fluid pipeline can only have one liquid. A fluid switch, which may be a solenoid valve, is inserted into the fluid line and controls the flow of liquid into the fluid center device. The dyeing module can be provided with a fluid middle-end device, a plurality of fluid inlets are distributed on the device for receiving liquid from outside, and a fluid switch is arranged on the inlet and can be an electromagnetic valve; the device has at least one fluid outlet, which may be provided without a switch. The fluid end device may be considered a liquid transfer station. The fluid medium end device has a liquid storage chamber therein. The staining module has at least one fluid end device therein. The fluid end device is connected with the fluid middle end device through a fluid pipeline. The fluid end device and the fluid middle end device realize liquid communication. When a switch in the fluid line in which a certain liquid is present is opened, the liquid flows into the fluid medium end device, so that the storage cavity of the fluid medium end device is filled with the liquid. To allow the liquid to flow into the end-of-fluid device, the air pressure within the reservoir of the end-of-fluid device may be reduced in some way. This method may be to use the pump 2800E to draw air from the chamber. When the liquid quantity in the storage cavity reaches a certain standard, a switch in a fluid pipeline where the liquid exists is closed, and the liquid is stopped from entering. In order to prevent the fluid middle end device from sucking excessive liquid, a liquid overflow outlet is arranged in the fluid middle end device. In operation of the fluid end-device, liquid in the storage chamber flows from the outlet of the fluid end-device, through the fluid end-device and onto the slide carried by the carrier tray 401. In order to make the liquid have a certain power, a pump is arranged on a pipeline between the fluid middle end device and the fluid end device to apply power to the liquid. During this process, the air pressure in the liquid storage chamber of the fluid center device is maintained equal to the atmospheric pressure by a certain method. When the amount of liquid on the slide reaches a desired level, the fluid end device changes operating conditions to flow the fluid end device and the liquid in the fluid line between the fluid end device and the fluid center device into the storage chamber of the fluid center device. The liquid in the storage chamber is discharged by a tube. After a certain time, the fluid end device removes liquid from the slide carried by the carrier tray 401 and delivers it to a waste recovery site (e.g., a waste reservoir). The task of the liquid is finished, the liquid which completes the task is discharged from the storage chamber by the fluid middle-end device, and the liquid is discharged out of the dyeing module through the corresponding fluid pipeline.
Fig. 7A is an isometric view of the mid-fluid end device and fig. 7B is a top view of the mid-fluid end device. 2201A-2201L are fluid switches, which may be solenoid valves. 2202 are central common chambers. The central common chamber has one or more inlets and one or more outlets through which a number of branch passages may be connected. The fluid switch is connected with each fluid pipeline and/or a negative pressure generating device (which can be a vacuum pump) and/or atmospheric pressure.
Figure 7C is an isometric view of a central common chamber. The central common chamber may be composed of a top, a waist and a bottom, each of which performs a different function. The central common chamber has a bottom and a waist, but may have one or more waists. The central common chamber has a top, a waist, and a bottom. The top has 5 openings, four of which are connected to the fluid switch and one of which is connected to the waist. The waist has 6 openings, four of which are connected to the fluid switch, and the other two of which are connected to the top, waist or bottom. The bottom may have 5-sided openings or 9-sided openings, of which 4-sided openings are the fluid outlets and one-sided openings are necessary to connect the waist. In this example, 2203 is the top of the central common chamber, 2204 is the waist of the central common chamber, and 2205 is the bottom of the central common chamber. 2206A are liquid outlets on the bottom 2205 of the central common chamber that connect to fluid lines leading to fluid end devices. The central common chamber top 2205 has two ports for connection to a negative pressure generating device, and one may be a liquid overflow and one may need to be connected to the atmosphere. The central common chamber waist 2204 is generally connected to a fluid line for liquids. When a certain liquid is needed, a fluid switch on a fluid pipeline of the liquid is turned on, and a switch connected with negative pressure is also turned on, so that the liquid enters the central public chamber. If the liquid enters too much, the liquid flows out from the liquid overflow outlet. When it is desired to deliver liquid from the central common chamber to the fluid end device, the switch to the atmospheric port is opened and the pump is started and liquid flows out of the central common chamber 2202.
Fig. 7D is a top view of the central common chamber. Fig. 7E is a B-B cross-sectional view of the central common chamber. 2207 is a conduit that is part of the top of the central common chamber. One end of the pipe is connected to the top and its length is such that its other end is just able to reach the bottom of the central common chamber. The tubing is connected through the top of the central common chamber to an external gas pressure controller, which may be a vacuum pump. The fluid medium end device changes the gas pressure in the liquid storage chamber through the pipeline. Meanwhile, the fluid middle-end device can also drain the liquid in the storage cavity for completing the task out of the storage cavity through the pipeline. When it is desired to drain the liquid in the central common chamber, a negative pressure is created in conduit 2207 and the switch to the atmosphere is opened and the liquid in the central common chamber can be drained from the fluid center device.
Fig. 7F is an isometric view of a fluid end device. Fig. 7G is a bottom view of the fluid end device. Fig. 7H is a front view of the fluid end device. Fig. 7I is a stepped cross-sectional view of G-G in a front view of the fluid end device. Fig. 7J is a cross-sectional view at the center of the fluid end device. 2301 is a fluid inlet head of a fluid end device, the fluid inlet head 2301 being connected to a fluid mid-end device by a fluid line. When a switch in the fluid line in which a certain liquid is present is opened, the liquid flows into the fluid medium end device, so that the storage cavity of the fluid medium end device is filled with the liquid. When the liquid quantity in the storage cavity reaches a certain standard, a switch in a fluid pipeline where the liquid exists is closed, and the liquid is stopped from entering. Subsequently, the fluid end device is activated and liquid in the storage chamber flows out of the outlet of the fluid middle end device through the fluid line to the fluid end device inlet head 2301 into the fluid end device. Reagent sequentially enters the common manifold 2308 and the lower tube via the inlet head 2301, accumulates a certain amount of liquid in the lower lumen for bubble prevention, and then flows out of the outlet 809 of the lower tube. 2302 is a waste outlet head of a fluid end device. The task-accomplishing liquid recovered from the slide eventually exits the fluid end device through the waste outlet head 2302 of the fluid end device. 2303 is a high pressure gas fitting of a fluid end device through which high pressure gas enters. The piping of the inlet header 2301 and the piping of the high pressure gas fitting 2303 merge into one common manifold 2308. Attached to the end of the common manifold is a drip needle 2305. In fig. 7G, 2304 is a waste liquid recovery hole. Waste liquid recovery holes 2304 are distributed in rows on both sides of a drip needle 2305. The number of the recovery holes of each waste liquid is between 5 and 15. The waste liquid recovery hole is connected with the negative pressure chamber. The negative pressure chamber is connected to a negative pressure source through a waste outlet head 2302, which can draw negative pressure through tubing to draw liquid out of the negative pressure chamber. When liquid is distributed, the waste liquid recovery hole 2304 and the high-pressure gas joint 2303 are in a non-working state, and the liquid passes through the liquid inlet head 2301 and the common manifold 2308 and finally goes out of the liquid dropping needle 2305 to the glass slide. When the liquid has completed the coloring task, the waste liquid recovery hole 2304 and the high-pressure gas joint 2303 start to operate. The high pressure gas enters the common manifold 2308 from the high pressure gas fitting 2303 and then travels in both directions. Upward flow, draining liquid in the conduit from the liquid inlet head 2301 out of the fluid end device to the fluid middle end device. Flows downward, out of the common manifold 2308 to the drip needles 2305 and blows liquid onto the slides. The high air pressure forces the liquid on the slide to spread from the center to both sides of the waste liquid recovery hole. Meanwhile, the low air pressure in the waste liquid recovery hole sucks the liquid into the negative pressure chamber, thereby completing the waste liquid removal work.
When the fluid end device dispenses reagent liquid onto the surface of the slide 402, the two-dimensional code 403 provided on the slide 402 can be identified by the identification device, labeled and the information is transmitted to the human-machine interaction system.
Fig. 8A is an isometric view of a portion of the dyeing module assembly. Figure 8B is an isometric view of a carrier tray receiving tray and a power device. Fig. 8C is a front view of the carrier tray receiving tray and the power device. In fig. 8A, 2900 represents a temperature maintaining means that radiates or radiates heat to maintain the temperature within the dyeing apparatus within a set temperature range or value. 401, a carrier tray carrying slides, the slides are circumferentially distributed on the carrier tray, and the axis of the slide in the longitudinal direction is aligned with the center of the tray. The central region of the carrier platter 401 has raised spatial geometries with corresponding pattern extraction features on the bottom surface of the carrier platter corresponding to those shapes. During the staining process, the fluid end devices 2300A-2300D can be precisely aligned with slides carried on the carrier tray. After the completion of a staining procedure, carrier tray receptacle 2401 rotates carrier tray 401 through an angle such that the next slide adjacent to the slide that completed the staining procedure is properly aligned with fluid end devices 2300A-2300D. The carrier tray 401 is coupled to the carrier tray receiving tray in a manner that maintains a relative position unchanged. 2401 of fig. 8B shows a carrier tray receiving tray. The carrier tray receptacle 2401 also has raised spatial geometries thereon, and these raised spatial geometries may be exactly combined with the pattern-drawing features on the bottom surface of the carrier tray. In one embodiment, a circular aperture 404 is provided in the carrier tray 401 that is coupled to a raised structure on the carrier tray receptacle 2401. The power device has a carrier rotation drive mechanism 2400 that drives the carrier tray carrier 2401 to rotate. The receiving receptacle rotational drive mechanism 2400 may be an electric motor. The carrier tray rotational drive mechanism 2400 and the carrier tray carrier 2401 may be connected to each other by a timing belt. The carrier tray lifting mechanism 2500 and the carrier tray carrying tray 2401 can be connected by a screw rod to realize the lifting linear motion of the carrier tray carrying tray 2401.
In fig. 8C 2400 shows a carrier tray receiving tray, 2500 shows a receiving tray lifting mechanism, and 2600 shows a frame of a dyeing apparatus. The carrier tray supports at least a lifting linear movement relative to the frame of the dyeing device and a circular movement relative to the frame of the dyeing device. The carrier tray receiving tray has two states of open and working. In the open state, the carrier tray receiving tray does not perform a circular motion. The carrier tray receiving tray only moves linearly during opening and during returning. In the working state, the carrier tray carrying tray only performs circular motion. In fig. 6, the carrier tray receiving tray is in an operative state. In the operational state, the carrier tray receiving tray can enable at least one slide on the carrier tray to face the fluid end device after each circular movement is stopped. Meanwhile, a device for detecting whether the position of the carrier tray for carrying the carrier is accurate or not is also arranged on the rack of the dyeing device. The power device provides power for the carrier tray bearing support. In fig. 8C, the carrier tray receiving tray is in an open state.
Automatic drop dyeing and sealing piece equipment with integrated machine structure
Referring to fig. 1 to 12, an embodiment of the present invention further provides an automated drip-dyeing and sealing apparatus having an integrated structure, including a frame 700, a carrier tray 401, a carrier transfer module 500, a heat treatment module 100, 900, an automated drip-dyeing module 200, and an automated sealing module 300, wherein the heat treatment module 100, 900, the automated drip-dyeing module 200, and the automated sealing module 300 are disposed on the frame 700, the carrier transfer module 500 includes an X-axis rail 503, a Y-axis rail 504, and a carrier tray carrying mechanism, the Y-axis rail 504 is mounted on the frame 700, the X-axis rail 503 is movably mounted on the Y-axis rail 504, the carrier tray carrying mechanism is used to carry the carrier tray 401, the carrier tray carrying mechanism is movably mounted on the X-axis rail 503, the carrier tray carrying mechanism and the X-axis rail 503 are respectively driven by a driving mechanism (e.g. a motor), and thereby the carrier tray 401 carrying a slide is carried to the heat treatment module 100, the automated drip-dyeing module is carried to the heat-treatment module 900, and then the heat-sealing module is carried to heat-treat the slide, and subsequently the heat-sealing module 900 is carried to heat-treat the slide, and then drip-sealing the slide is carried to the heat-treat. The automatic drip-dyeing module 200 may be the automatic drip-dyeing module 200 provided by the invention.
In a preferred embodiment, the frame 700 is in a vertical structure, the X-axis guide rails 503 are arranged along a horizontal direction, the Y-axis guide rails 504 are arranged along a vertical direction, and the heat treatment modules 100, 900, the automatic drip dyeing module 200 and the automatic sealing sheet module 300 are distributed at different positions on the frame 700 or at different horizontal positions on the same height.
In a preferred embodiment, the heat treatment modules 100, 900 include first to second heat treatment modules, the automatic drop dyeing module 200 includes first to third drop dyeing modules 200, the first heat treatment module 900 is used for dewaxing heat treatment, the second heat treatment module 100 is used for heat curing treatment of the sealing agent, and the first to third drop dyeing modules 200 can respectively perform drop dyeing treatment on one carrier disc 401 at the same time.
In a preferred embodiment, the X-axis rail 503 is slidably mounted to the Y-axis rail 504 by a first slider 505, and the carrier disk carrying mechanism is slidably mounted to the X-axis rail 503 by a second slider 502.
In a preferred embodiment, the apparatus further comprises a carrier tray temporary storage module 600, wherein the carrier tray temporary storage module 600 is disposed on the frame 700, and is used for receiving the dewaxed and heated carrier tray 401 transported by the carrier transport module 500 for temporary storage when the automatic drop dyeing module 200 has no empty space temporarily.
In a preferred embodiment, the carrier tray carrying mechanism comprises a cantilevered outwardly extending fork-type structure 506, the bottom of the carrier tray 401 is provided with a positioning structure cooperating with the fork-type structure 506, and the carrier tray 401 is carried on the fork-type structure 506 by coupling the positioning structure 408 with the fork-type structure 506. Preferably, the positioning structure 408 protrudes from the bottom surface of the carrier plate 401, and is embedded in the middle of the fork-shaped structure 506 when coupled with the fork-shaped structure 506, so as to position the carrier plate 401. The fork-like structure may be, but is not limited to, a U-shaped structure.
In a preferred embodiment, the rack 700 further comprises a carrier tray access module, the carrier tray access module is a retractable structure 406 arranged at the carrier tray access, the retractable structure 406 is arranged above the initial position of the carrier tray carrying mechanism, two notches aligned with the extending fingers of the fork-shaped structure 506 are arranged on the retractable structure 406, when the carrier tray 401 is arranged on the retractable structure 406, at least one part of the two notches is covered, and when the carrier tray carrying mechanism moves upwards, the fork-shaped structure 506 passes through the two notches to transfer the carrier tray 401 from the retractable structure 406 to the carrier tray carrying mechanism; and/or
In a preferred embodiment, the tray receiving module further includes a tray receiving module, where the rack 700 is provided with a tray outlet, the tray receiving module is a retractable structure 406 disposed at the tray outlet, the retractable structure 406 is disposed below the end position of the tray carrying mechanism, two notches aligned with the extending fingers of the fork-shaped structure 506 are disposed on the retractable structure 406, at least a portion of the two notches are covered when the tray 401 is disposed on the retractable structure 406, and when the tray carrying mechanism moves downward, the fork-shaped structure 506 passes through the two notches to transfer the tray 401 from the tray carrying mechanism to the retractable structure 406.
In a preferred embodiment, the carrier plate 401 is provided with a positioning hole 404, and the telescopic structure 406 is provided with a positioning protrusion 407 matched with the positioning hole.
In a preferred embodiment, the automatic drip-dyeing module 200 includes a drip-dyeing device, a carrier tray receiving tray for receiving the carrier tray 401 transported by the carrier transfer module 500, a receiving tray lifting mechanism for lifting and moving the carrier tray receiving tray between a receiving position of the carrier tray 401 and a drip-dyeing processing position, and a receiving tray rotation driving mechanism for driving the carrier tray receiving tray to rotate so that the drip-dyeing device performs a drip-dyeing process on different slides on the carrier tray 401; preferably, the carrier tray 401 is provided with a positioning hole, and the carrier tray receiving tray is provided with a positioning protrusion matched with the positioning hole.
In a preferred embodiment, the thermal processing module 100, 900 includes a heating device for receiving the carrier tray 401 transported by the carrier transfer module 500, a carrier tray receiving tray for lifting and moving the carrier tray receiving tray between a receiving position of the carrier tray 401 and a thermal processing position, and a receiving tray lifting mechanism for providing a heat source for thermal processing; preferably, the carrier tray 401 is provided with a positioning hole, and the carrier tray receiving tray is provided with a positioning protrusion matched with the positioning hole.
In a preferred embodiment, a liquid storage module provided on the frame 700 for supplying liquid to the automatic drip dyeing module 200 and a waste liquid collection module for collecting waste liquid from the automatic drip dyeing module 200 are further included.
Specific embodiments are described further below.
Referring to fig. 1 to 12, in one embodiment, the frame 700, the carrier tray 401, the carrier tray access module 400, the carrier transfer module 500, the automatic drip dyeing module 200, the liquid storage module 800, the heat treatment modules 100 and 900 (the constant temperature heating curing system and the drying dewaxing system), the waste liquid collection module, the automatic sealing module 300 and the man-machine interaction control system are integrated. The integrated machine of the automatic drop dyeing treatment system for the biological tissue sample slices adopts a distributed layout, so that the treatment efficiency can be accelerated.
Optionally, the automated drop-dyeing processing system for biological tissue sample slices further comprises: an anti-pollution module and an alarm processing module.
Referring to fig. 1, in a preferred embodiment the stand 700 is vertical. Optionally, the integrated machine of the automatic drip dyeing treatment system for the biological tissue sample slices can be a horizontal box type and a separate box type.
Referring to fig. 1, in a preferred embodiment, three drop dyeing modules 200 and two heat treatment modules 100, 900 are provided in order to improve the efficiency of parallel processing according to dyeing time and heat treatment time.
Referring to fig. 2 and 4, in a preferred embodiment, the carrier transport module 500 includes a two-dimensional guide rail 504 and motion sliders 505, 502 to enable the carrier tray 401 to move between the modules of the automatic drip dye module 200, the thermal processing modules 100, 900, and the automatic die block module 300, the process comprising:
the transport carrier plate 401 moves between the automatic drip dye module 200, the thermal processing modules 100, 900, the automatic film sealing module 300;
the round hole 404 at the bottom of the carrier plate 401 is fixedly coupled with the convex structure 407 of the telescopic structure 406, the telescopic structure 406 is contracted, the motor controls the movement of the moving slide block 502, the fork-shaped structure 506 of the motor is fixedly coupled with the U-shaped positioning structure 408 at the bottom of the carrier plate 401, and the motor controls the moving slide blocks 505 and 502 to drive the carrier plate 401 to move;
Carrying the carrier plate 401 into the integrated machine from the inlet of the integrated machine, and carrying the carrier plate 401 from the integrated machine to the outlet of the integrated machine;
the automatic drip dyeing module 200 moves the carried carrier plate 401 into the module for processing, and moves out after processing;
the heat treatment module 100, 900 moves the carrier tray 401 into the module for treatment and moves out after treatment;
the automated tape-out module 300 moves the carrier tape 401 from transport into the module for processing and out after processing.
Referring to fig. 3, in a preferred embodiment, a carrier tray 401 holds slides 402 by a movable clamp slot 405, slides 402 can be removed from carrier tray 401, and multiple slides 402 can be held on carrier tray 401 simultaneously. The slide 402 has a two-dimensional code thereon that can be recognized by a recognition device.
Referring to fig. 3, in a preferred embodiment, the carrier plate 401 is in a symmetrical shape such as a circular shape or a rectangular shape, and the slides 402 are regularly distributed on the carrier plate 401, which is advantageous for the pipelining process.
Referring to fig. 5, in a preferred embodiment, a pair of miniature cameras are arranged at the upper part of the integrated machine to monitor each module in the integrated machine, images are transmitted to a man-machine interaction interface for display, an infrared scanner scans a two-dimensional code 403 on a glass slide 402 to obtain basic information of a patient and processing flow information of tissue slices, data are transmitted to and transmitted into a host machine, a photosensitive sensor judges whether the glass slide 402 is correctly placed or not by receiving signals, and the data are transmitted to the man-machine interaction system
Referring to fig. 6-8C, the automatic drip-dye module 200 of the preferred embodiment is described above in detail.
Referring to fig. 9A-9F, the thermal processing modules 100, 900 of the preferred embodiments are described in detail below.
Heat treatment module and automatic drop dyeing sealing piece equipment with same
Referring to fig. 9A to 9F, an embodiment of the present invention further provides a heat treatment module, which can be used in the aforementioned automated drip-dyeing and sealing device for heat treatment of glass slides. The heat treatment module comprises a semi-closed cavity 901, a plurality of heating resistors 907, blower fan blades 912 and a blower driving mechanism, wherein the heating resistors 907 and the blower fan blades 912 are arranged in the semi-closed cavity 901, the heating resistors 907 are circumferentially distributed in the semi-closed cavity 901, the upper end of the semi-closed cavity 901 is closed and provided with a bottom opening, a carrier tray 401 carrying a plurality of glass slides can be matched with the semi-closed cavity 901 at the bottom opening to form a closed cavity, the air in the cavity is heated when the heating resistors 907 are electrified, and the blower driving mechanism is used for driving the blower fan blades 912 to rotate so as to form annular heat convection in the cavity, so that the plurality of glass slides on the carrier tray 401 are uniformly heated by convection.
In a preferred embodiment, the plurality of heating resistors 907 are uniformly distributed in a ring shape.
In a preferred embodiment, the heat treatment module further comprises a plurality of heating partitions 906 disposed in the semi-enclosed cavity 901 in a ring shape and vertically distributed, the plurality of heating partitions 906 radially extend outwards around the center of the cavity and are connected to a fixed outer ring, and the plurality of heating resistors 907 are respectively mounted on the different heating partitions 906.
In a preferred embodiment, the thermal processing module further comprises a plurality of temperature sensors 908 uniformly disposed within the cavity to generate feedback signals for adjusting the operating state of one or more heating resistors 907 at corresponding positions when the plurality of temperature sensors 908 detect that the temperature within the cavity is not uniform or is too high, so as to maintain the temperature within the cavity uniform and stable.
In a preferred embodiment, the plurality of temperature sensors 908 are disposed on different heating partitions 906 corresponding to the plurality of heating resistors 907, respectively.
In a preferred embodiment, the heat treatment module further includes a rotating bracket 911 disposed in the cavity, the rotating bracket 911 includes a rotating shaft 913, and an upper bracket plate and a lower bracket plate coupled to the rotating shaft 913, the blower fan blade 912 includes a plurality of fan blade units distributed in a vortex shape around a center of the rotating bracket 911, the plurality of fan blade units are vertically fixed between the upper bracket plate and the lower bracket plate, the rotating bracket 911 is disposed at an upper side or a lower side of the plurality of heating partitions 906, or an upper layer and a lower layer of the plurality of heating partitions 906 are disposed in the cavity, and the rotating bracket 911 is disposed between the plurality of heating partitions 906 at an upper layer and the plurality of heating partitions 906 at a lower layer.
In a preferred embodiment, the blower drive mechanism includes a motor 903, a drive belt 904, and a drive gear 905, the motor 903 being coupled to the rotating shaft through the drive gear 905 and the drive belt 904.
In a preferred embodiment, the thermal processing module further comprises a carrier tray receiving tray 909 and a receiving tray lifting mechanism 910, the carrier tray receiving tray 909 being configured to receive a transported carrier tray 401, the receiving tray lifting mechanism 910 being configured to lift the carrier tray receiving tray 909 between a receiving position of the carrier tray 401 and the bottom opening of the semi-enclosed cavity 901.
In a preferred embodiment, the carrier tray 401 is provided with a positioning hole, such as a circular hole 404, and the carrier tray receiving tray 909 is provided with a protrusion 914 that mates with the positioning hole.
In a preferred embodiment, the semi-enclosed cavity 901 is formed by a cylindrical housing that is open at the bottom and that covers the plurality of heating resistors 907 and the blower blades.
Specific embodiments are described further below.
Referring to fig. 9A-9F, in a preferred embodiment, the thermal processing module 100, 900 includes a semi-enclosed cavity 901, a motor 903, a drive belt 904, a drive gear 905, a heating partition 906, a heating resistor 907, a temperature sensor 908, a carrier tray receiving tray 909, a thermal processing module vertical telescoping device 910, a rotating mount 911, and blower fan blades 912.
A plurality of layers of heating resistors 907 distributed annularly are arranged in the semi-closed cavity 901 and connected with the driving circuit integrated module. The semi-enclosed cavity 901 employs a cylindrical housing into which the heating portion is embedded. Temperature sensors 908 are also uniformly disposed within the cylindrical housing, the values of which are fed back to the drive circuit integrated module. The layer of annularly distributed heating resistors 907 is matched with a built-in rotating bracket 911, and the rotating bracket 911 is provided with annularly radiation distributed blower fan blades 912. The semi-enclosed cavity 901 may be mated with the carrier platter 401 to form an enclosed cavity.
The working mode is as follows: the heating resistor 907 works, and the motor 903 drives the transmission belt 904 to drive the rotary bracket 911 to rotate, so that the blower fan blade 912 is driven to rotate, annular heat convection is formed, and the temperature in the semi-closed cavity is uniformly heated and increased. The temperature is fed back to the driving circuit integrated module by the temperature sensor 908, and the heating temperature distribution is finally kept uniform and stable by adjusting the heating resistor 907 for the part with uneven temperature or over-high temperature.
The carrier tray 401 may be transported to the thermal processing modules 100, 900 by a carrier transport module. Under the transportation of the carrying and transferring module 500, the round hole 404 at the bottom of the carrier tray 401 is coupled and fixed with the convex structure 914 of the carrier tray supporting and supporting device 909 in the heat treatment module 100, 900, then the supporting and supporting device lifting mechanism 910 of the heat treatment module ascends to enable the carrier tray supporting and supporting device 909 to lift the carrier tray 401, the carrier tray 401 is matched with the bottom opening of the semi-closed cavity 901 to form a closed cavity, the carrier tray 401 carries the glass slide 402, and the glass slide 402 is heated for a certain time in the closed cavity through annular heat convection. Before the glass slide 402 is dripped, the dewaxing treatment is carried out by convection baking at 140 ℃ for 4 minutes at the heat treatment module 900, after the dewaxing of the glass slide 402 is finished, the glass slide 402 is dripped and sealed, and then the glass slide is conveyed to the heat treatment module 100 to be cured by sealing at 70 ℃ for 5 minutes.
In the convection baking process, the paraffin cover on the surface of the biological tissue sample on the slide 402 on the carrier tray 401 is heated to a liquid state and dropped under the action of gravity. After the slide 402 is drip-stained by the automatic drip-stain module, the automatic coverslip module adds a coverslip reagent to the surface of the slide 402 and covers the coverslip. In the curing process, the sealing agent can be rapidly cured by heating at 70 ℃ for 5 minutes. The sheeting agent is a liquid agent such as a cyanoacrylate adhesive or a photo-curable adhesive polymer.
Man-machine interaction module and automatic drop dyeing and sealing piece equipment with same
Referring to fig. 1A to fig. 1C, and fig. 10 to fig. 12, an embodiment of the present invention further provides an automatic drip-dyeing and sealing device with a man-machine interaction function, which includes a rack, a carrier tray, a carrying and transferring module, a heat treatment module, an automatic drip-dyeing module, an automatic sealing module, a man-machine interaction module, and a control unit, wherein the carrying and transferring module is used for carrying the carrier tray on the rack, the heat treatment module, the automatic drip-dyeing module, and the automatic sealing module are disposed on a carrier tray carrying route on the rack, the carrier tray carries a slide, the heat treatment module is used for dewaxing and heating and solidifying a sealing agent for the slide, the automatic drip-dyeing module is used for drip-dyeing the slide, the automatic sealing module is used for sealing the slide, the control unit controls an operation flow and a processing procedure of each module, the man-machine interaction module includes a man-machine interaction interface, and the man-machine interaction module is connected to the control unit for controlling input of instructions and output of processing information.
In a preferred embodiment, the automated drop dye sealing apparatus further comprises a monitoring and identification system comprising one or more of the following: the camera is arranged on the rack and used for monitoring each module, and monitoring images are transmitted to the man-machine interaction interface for display; the infrared scanner is arranged on the frame and is used for scanning the two-dimensional code on the glass slide to acquire sample information and processing flow information of the tissue slice corresponding to the glass slide, transmitting the acquired information to the control unit, and controlling the operation flow and the processing process of each module by the control unit according to the acquired information; and the photosensitive sensor is arranged on the rack and is used for detecting whether the glass slide is correctly placed, transmitting detection signals to the control unit and processing or alarming by the control unit.
Specific embodiments are described further below.
The man-machine interaction module comprises a man-machine interaction interface which is interacted with the control host, the running flow and the processing process of the biological tissue sample slice drop-dyeing sealing sheet are adjusted by inputting control instructions to the micro-processor, the processing comprises two-dimensional platform moving, stopping, drop moving and closing, drop reagent adding sequence, micro-adjustment of the micro-two-dimensional sliding table and monitoring of identification signal processing, the processing of different biological tissue sample slices is operated and designed through the man-machine interaction interface, the directional batch processing is carried out on various biological tissue sample slices, and the dyeing flow is selected according to the requirements through the man-machine interaction system, so that the effects of accuracy, high efficiency, no pollution and reagent saving are achieved. The man-machine interaction interface comprises a display and an input device, and is used for controlling the input of instructions and the output of information. The upper part of the frame of the all-in-one machine is provided with a pair of miniature cameras which are used for monitoring all modules in the all-in-one machine, and images are transmitted to a human-computer interaction interface for display. The infrared scanner is used for scanning a two-dimensional code on the glass slide to obtain tissue slice sample information (such as patient basic information) and tissue slice processing flow information, transmitting data to and transmitting the data into the host, and the photosensitive sensor judges whether the glass slide is correctly placed or not by receiving signals or not and transmits the data into the human-computer interaction system.
The automated drop dye sealing apparatus of the various preferred embodiments is described in detail above.
The background section of the present invention may contain background information about the problems or environments of the present invention and is not necessarily descriptive of the prior art. Accordingly, inclusion in the background section is not an admission of prior art by the applicant.
The foregoing is a further detailed description of the invention in connection with specific/preferred embodiments, and it is not intended that the invention be limited to such description. It will be apparent to those skilled in the art that several alternatives or modifications can be made to the described embodiments without departing from the spirit of the invention, and these alternatives or modifications should be considered to be within the scope of the invention. In the description of the present specification, reference to the terms "one embodiment," "some embodiments," "preferred embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Those skilled in the art may combine and combine the features of the different embodiments or examples described in this specification and of the different embodiments or examples without contradiction. Although embodiments of the present invention and their advantages have been described in detail, it should be understood that various changes, substitutions and alterations can be made herein without departing from the scope of the invention as defined by the appended claims.
Claims (7)
1. An automatic drop dyeing and sealing device comprises an automatic drop dyeing module for automatically dyeing a biological tissue sample slide and an automatic sealing module for sealing the dyed slide, and is characterized in that the automatic drop dyeing module comprises a fluid middle end device, a fluid end device, a carrier disc and a carrier disc bearing support, wherein the carrier disc is used for bearing the slide, the carrier disc is borne and held by the carrier disc bearing support, the fluid middle end device is connected with the fluid end device through a fluid pipeline, the fluid middle end device distributes externally input dyeing liquid to the fluid end device, the fluid end device is provided with a liquid outlet and a waste liquid recovery hole, the dyeing liquid is applied to the slide borne by the carrier disc through the liquid outlet, and the dyeing liquid is recovered to a negative pressure cavity through the waste liquid recovery hole in a negative pressure suction mode after dyeing of the sample on the slide is finished; the fluid middle-end device comprises a combination body formed by a top part, at least one waist part and a bottom part which are overlapped together in a square shape, wherein a liquid storage cavity is formed by the combination body, a plurality of side surfaces of the top part and a bottom surface connected with the waist part are respectively provided with openings, a plurality of side surfaces of the at least one waist part are respectively provided with openings, the top surface of the waist part connected with the top part is provided with an opening, the bottom surface of the waist part connected with the bottom part is provided with an opening, a plurality of side surfaces of the bottom part and the top surface connected with the waist part are respectively provided with an opening, the openings of the top part are used for communicating liquid or ventilation, and the openings of the waist part and the bottom part are used for communicating liquid; the automatic drop dyeing and sealing device comprises a glass slide, a heat treatment module, a plurality of heating resistors, a blower fan blade and a blower driving mechanism, wherein the heat treatment module is used for carrying out heat treatment on glass slides in automatic drop dyeing and sealing device and comprises a cylindrical semi-closed cavity, a plurality of heating resistors, the blower fan blade and the blower driving mechanism, the plurality of heating resistors and the blower fan blade are arranged in the semi-closed cavity, the plurality of heating resistors are circumferentially distributed in the semi-closed cavity, the upper end of the semi-closed cavity is closed and provided with a bottom opening, a circular carrier tray carrying a plurality of glass slides can be matched with the semi-closed cavity at the bottom opening to form a closed cylindrical cavity, the plurality of heating resistors heat air in the cavity when being electrified, and the blower driving mechanism is used for driving the blower fan blade to rotate so as to form annular heat convection in the cavity, so that the circular distribution of the plurality of glass slides on the carrier tray can be heated by uniform convection; the semi-closed type air blower comprises a semi-closed cavity, a plurality of heating partition plates, a plurality of annular rotating support and a plurality of air blower fan blades, wherein the plurality of heating partition plates are arranged in the semi-closed cavity in an annular vertical mode, the plurality of heating partition plates extend outwards in a radiating mode around the center of the cavity and are connected to a fixed outer ring, the plurality of heating resistors are respectively arranged on different heating partition plates, the semi-closed type air blower fan comprises a rotating shaft, an upper support plate and a lower support plate which are coupled to the rotating shaft, the air blower fan blades comprise a plurality of fan blade units which are arranged around the center of the rotating support in an eddy mode along the circumferential direction of the cavity, the plurality of fan blade units are vertically fixed between the upper support plate and the lower support plate, the rotating support is arranged on the upper side or the lower side of the plurality of heating partition plates, or the upper layer and the lower layer of heating partition plates are arranged in the cavity, and the rotating support is arranged between the plurality of heating partition plates on the upper layer and the lower layer.
2. The automated drip dye sealing apparatus of claim 1, further comprising a power device including a carrier rotation drive mechanism coupled to the carrier tray carrier for driving the carrier tray carrier to rotate in a working position to position a slide to be dyed relative to the fluid end device and a carrier lift mechanism for lifting the carrier tray carrier between a carrier tray carrier position and a drip dye processing position.
3. An automated drop dyeing apparatus according to claim 1 or claim 2, wherein the fluid end means comprises a liquid inlet head connected to the liquid outlet via a common manifold and a high pressure gas connector connected to the fluid mid-end means via a fluid line, the high pressure gas connector being adapted to receive high pressure gas during liquid waste recovery, a plurality of the liquid waste recovery holes being distributed around the liquid outlet.
4. The automated drop dye sealing apparatus of any of claims 1-2, further comprising a pump between the in-fluid end device and the fluid end device.
5. The automated drop dyeing apparatus of any of claims 1-2, wherein the fluid mid-end device comprises a liquid storage chamber provided with a fluid inlet for inputting dyeing liquid, a fluid outlet for dispensing liquid to the fluid end device, and at least one of: a negative pressure suction port for forming a negative pressure in the liquid storage chamber so as to input a staining liquid, a liquid overflow port for overflowing the liquid when the liquid is input excessively, an atmosphere connection port for communicating with the atmosphere when the liquid is dispensed to the fluid terminal device, and a discharge port for discharging the liquid after the staining of the sample is completed; the liquid storage chamber is provided with a plurality of fluid inlets; the fluid inlet is provided with a fluid switch; the negative pressure suction port and the exhaust port are the same.
6. The automated drop dyeing apparatus of claim 5, wherein the fluid mid-end device is provided with a negative pressure conduit connected to the negative pressure suction port and extending to a bottom of the liquid storage chamber.
7. The automated drop dye sealing apparatus of any one of claims 1-2, further comprising a plurality of temperature sensors disposed uniformly within the cavity to generate feedback signals for adjusting the operating state of one or more heating resistors at respective locations to maintain uniform and stable temperature within the cavity when the plurality of temperature sensors detect that the temperature within the cavity is non-uniform or too high; the plurality of temperature sensors are respectively arranged on different heating partition boards corresponding to the plurality of heating resistors.
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| CN202011601497.4A CN112730000B (en) | 2020-12-29 | 2020-12-29 | Automatic change drop dyeing sealing piece equipment |
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| CN202011601497.4A CN112730000B (en) | 2020-12-29 | 2020-12-29 | Automatic change drop dyeing sealing piece equipment |
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| CN112730000B true CN112730000B (en) | 2023-06-20 |
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| US11249095B2 (en) * | 2002-04-15 | 2022-02-15 | Ventana Medical Systems, Inc. | Automated high volume slide processing system |
| EP4095509A1 (en) * | 2013-12-13 | 2022-11-30 | Ventana Medical Systems, Inc. | Automated histological processing of biological specimens and associated technology |
| WO2017218882A1 (en) * | 2016-06-16 | 2017-12-21 | Nanocytomics, LLC | Automated staining system |
| CN209589628U (en) * | 2019-03-08 | 2019-11-05 | 江苏硕世生物科技股份有限公司 | Slide sample introduction, goes out sampling device and slide automatic staining device at dyeing |
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