CN208505894U - A kind of refractometer inductive probe - Google Patents

A kind of refractometer inductive probe Download PDF

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Publication number
CN208505894U
CN208505894U CN201821296238.3U CN201821296238U CN208505894U CN 208505894 U CN208505894 U CN 208505894U CN 201821296238 U CN201821296238 U CN 201821296238U CN 208505894 U CN208505894 U CN 208505894U
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China
Prior art keywords
refractometer
inductive probe
filter device
detecting electrode
air nozzle
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CN201821296238.3U
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Chinese (zh)
Inventor
谢昌贤
刘运添
王月清
刘迎春
菅明生
张常德
王鹏飞
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JINHE BIOTECHNOLOGY CO Ltd
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JINHE BIOTECHNOLOGY CO Ltd
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Abstract

The utility model relates to a kind of refractometer inductive probes, belong to quick detection device technical field, which includes detecting electrode;Filter device, the filter device have cavity, and surround and be provided with filter structure on each face of the cavity, and filter device is set in the front end of the detecting electrode;Air nozzle, the air nozzle, which is arranged on the filter device, filters the detection liquid for entering the cavity through the filter structure for input air to remove;Steam jet, the steam jet are arranged on the filter device for inputting steam to clean the detection end of the detecting electrode.Utilize the refractometer inductive probe; it can be realized filtering-sampling-detection-reflux automation; guarantee the instantaneity of detection and the normalization of fermentation process; accurate, real-time on-line monitoring is accomplished to fermentation liquid whole process; and then online feed supplement is precisely instructed; personnel labor intensity is reduced, reducing leads to the waste of material because not stopping sampling.

Description

A kind of refractometer inductive probe
Technical field
The utility model belongs to quick detection device technical field, and in particular to a kind of refractometer inductive probe.
Background technique
It is well known that, as microorganism constantly breeds, energy source (culture medium) is also in microbial fermentation production process It is consumed constantly, if supplemented not in time, the growth and breeding of microorganism will slow down or stagnate, but be not that feed supplement is mended more It is much better, it because feed supplement is too many, will cause overnutrition, will affect the normal breeding and metabolism of microorganism instead.Culture medium needs How much supplement is just to know to train in fermentor according to the instant sugared content value in the fermentation liquid measured using sugared content as foundation The amount that base is short of is supported, fills into required culture medium in time by feeding-system.The standard of feed supplement amount will be directly affected due to surveying sugar True property, and feed supplement influences the quality of fermentation liquid, so sugared content value becomes one for fermentation success or failure in accurate detection fermentation liquid A key point.
China's fermentation industry occupies an important position in national economy, but overall technology level is compared with developed countries also Biggish gap, that there are raw material availabilities and conversion ratio is low, energy consumption is high, the pollution problems such as again.It is mould in soil both at home and abroad at present It is still after using traditional timing sampling with potassium ferricyanide method, anthracene copper ratio that methods and techniques used in sugar are surveyed in plain fermentation process Color method or fehling reagent titration.Wherein, fehling reagent titration specifically: polysaccharide generates glucose, grape after sour water solution It can be by the Cu in fehling reagent under aldehyde radical alkaline condition in sugar2+It restores and generates copper oxidule precipitation, it is remaining in fehling reagent Cu2+Iodine is precipitated with excessive iodate nak response in acid condition, iodine can use sodium thiosulfate standard solution direct titration, according to sulphur The dosage of sodium thiosulfate can find out the content of total reducing sugar in sample indirectly.However this round-about way seems time-consuming and laborious, obtains Data also can only as " out-of-date reference " cannot in time, effectively, fermentation liquid sugar amount value in true reflection tank, because according to It is to need the regular hour that the numerical value measured in this way, which calculates the amount that culture medium in fermentor requires supplementation with, so calculating at this time Feed supplement amount be not the amount really needed in tank in fact, this always slow half operation clapped undoubtedly will affect fermenting and producing Quality and yield, also affect entire fermentation industry fermentation level certainly.
Intermittent sample detection exists as following drawbacks: (1) large labor intensity of personnel;(2) continuous sampling causes to expect The waste of liquid with;(3) the sugar amount value that method measures according to this instructs feed supplement accuracy poor.So being badly in need of a kind of faster more convenient and fast method It realizes in fermentation process to the on-line monitoring of sugar.
Summary of the invention
In view of this, the purpose of this utility model is to provide a kind of refractometer inductive probes.
In order to achieve the above objectives, the utility model provides the following technical solutions:
1, a kind of refractometer inductive probe, the inductive probe include detecting electrode 1, filter device 2,3 and of air nozzle Steam jet 4;The filter device 2 has cavity 5, is provided with filter structure 6 on 5 outer wall of cavity, filter device 2 is set in institute State the front end of detecting electrode 1;The air nozzle 3 is arranged on the filter device 2 for input air to remove described in warp Filter structure filtering enters the detection liquid of the cavity;The steam jet 4 is arranged on the filter device 2 to be steamed for inputting Vapour is to clean the detection end of the detecting electrode 1.
Preferably, the cavity 5 is constituted from lid 7 and with the ontology 8 with lid 7, the detecting electrode 1, air spray Mouth 3 and steam jet 4 are arranged on the lid 7, and the filter structure 6 is arranged on the ontology 8.
Preferably, the ontology 8 includes filtering collet 9 and spacer screen pressure ring 10, and the filtering collet 9 includes side and bottom Face, the filter structure 6 are separately positioned on the side and bottom surface;The side is with apertured double-layer structure and interval Hollow out, the gap are used for the filter structure 6 being fixed on the side;The bottom surface hollow out passes through the spacer screen pressure Ring 10 is fixed at the filter structure 6 on the bottom surface.
Preferably, the filter device 2 is in cylindrical body.
Preferably, the filter structure 6 is stainless (steel) wire.
Preferably, the air nozzle 3 and steam jet 4 are arranged around the detecting electrode 1, and are located at different circumference On, the quantity of the air nozzle 3 is more than or equal to 2;The quantity of the steam jet 4 is more than or equal to 1.
Preferably, the air nozzle 3 and steam jet 4 are arranged around the detecting electrode 1, and are located at same circumference On, the quantity of the air nozzle 3 is more than or equal to 2;The quantity of the steam jet 4 is more than or equal to 1.
The utility model has the beneficial effects that: the utility model provides a kind of refractometer inductive probe, by existing There is refractometer inductive probe housing setting tool to have the filter device for being provided with filter structure on cavity and outer wall, it is ensured that detecting It can be effectively filtered the thick substances in hair loss zymotic fluid in the process, contact inductive probe with clear detection liquid, Jin Erti High detection precision.Meanwhile air nozzle is set on filter device, generated pressure when by input filtrated air, with It removes and enters the detection liquid of cavity through filtering structured filter, and then achieve the purpose that on-line real-time measuremen, in addition, filter device is also It is provided with steam jet, steam is inputted to clean the detection end of detecting electrode by the spray head, is further ensured that detection is accurate Degree.In addition, on the one hand can guarantee uniform force inside and outside the inductive probe, another aspect energy when filter device is in cylindrical body The flow resistance for enough reducing fermentation liquid in fermentor, keeps its flowing more smooth, not will form local turbulence, last cylindrical body is smooth Appearance be easier clean, disinfection do not stay dead angle.Using the refractometer inductive probe, filtering-sampling-detection-can be realized The automation of reflux guarantees the instantaneity of detection and the normalization of fermentation process, to fermentation liquid whole process accomplish it is accurate, in real time exist Line monitoring, and then online feed supplement is precisely instructed, personnel labor intensity is reduced, reducing leads to the wave of material because not stopping sampling Take.
Detailed description of the invention
In order to keep the purpose of this utility model, technical scheme and beneficial effects clearer, the utility model provides as follows Attached drawing is illustrated:
Fig. 1 is refractometer inductive probe schematic diagram in the utility model;
Fig. 2 is refractometer inductive probe perspective view in the utility model;
Fig. 3 is refractometer inductive probe top view in the utility model (omitting lid).
Fig. 1 is into Fig. 3,1- detecting electrode;2- filter device;3- air nozzle;4- steam jet;5- cavity;6- filtering Structure;7- lid;8- ontology;9- filters collet;10- spacer screen pressure ring.
Specific embodiment
The preferred embodiment of the utility model will be described in detail below.
As illustrated in the accompanying drawings from 1 to 3, the element numbers in attached drawing respectively indicate refractometer inductive probe used in embodiment: 1- detecting electrode;2- filter device;3- air nozzle;4- steam jet;5- cavity;6- filter structure;7- lid;8- ontology; 9- filters collet;10- spacer screen pressure ring.
In the utility model refractometer inductive probe using when be dipped in fermentation liquid completely, due in fermentation process, fermentation It is a kind of mixing state under stirring system work from beginning to end in tank, so the fermentation broth stream when a certain moment is filled through filtering 2 are set, by the filter structure 6 being arranged thereon, material moment has carried out solid- liquid separation, clear to enter filtering dress to liquid It sets in 2 cavity 5, sticky material is trapped within outside, and simultaneously detecting electrode 1 is sampled, empty after the completion of sample measurement Detection liquid in chamber 5 is blown out by the filtrated air that air nozzle 3 inputs, meanwhile, test side of the steam jet 4 to detecting electrode 1 Head is cleaned, and cleaning solution is blown out by filtrated air together with detection liquid, that is, completes the detection to that moment fermentation liquid, filtrate It is again and then filtering-sampling-detection-reflux after reflux, so circulation carries out above step, continuous to complete A series of detection operations.
Embodiment
Refractometer inductive probe in the utility model is used for terramycin fermentation process and surveys sugar online, realizes accurate feed supplement
It chooses one group of fermentor (one group includes three fermentors), installation kit contains refractometer sense in the present invention respectively on tank The refractometer that should be popped one's head in, and refractometer and feeding-system are subjected to data linkage, feed back to feeding-system according to refractometer Data correspondingly provide optimal culture medium and wait for benefit amount and add in fermentor, the index of refraction of the refractometer be nD ± 0.0002, the operating temperature of optical core components is -20-150 DEG C in refractometer inductive probe, and filter structure is in inductive probe 304 stainless (steel) wires of 150 mesh of aperture, inductive probe is protruded into tank, and rest part is isolated in outer by tank skin.
In addition one group of fermentor (one group include three fermentors) is taken again, refractometer is not installed, and during later stage fermentation, is led to The mode for crossing artificial detection detects sugared content in fermentation liquid.
Above-mentioned two groups of fermentors are used for the terramycin fermenting and producing of three groups of six batches, specifically: it is prepared in fermentor After culture medium needed for production, " disappearing in fact " sterilization treatment is done to entire tank, when tank temperature drops to technique required value, is moved from seeding tank To fermentor, fermentation starts kind, and two groups of fermentors remove whether installing different on refractometer, other all structures and specification additional All the same, parameter of materials used is the same in two groups of fermentors, and it is same with batch for accessing production strain used in two groups of fermentors One strain, and access amount is identical, and the control parameters such as two groups of fermentor production cycles, ventilatory capacity, pH value are all the same.Fermentation process In compile related data, the results are shown in Table 1 to 3, carry out bioactivity calculating, and the sugared, feed supplements to two kinds of survey using formula (1) Method controls lower fermentation level and compares.
In formula:
A: fermentation liquid puts tank unit (that is: potency), is indicated with u/ml.
V: fermentation liquid puts the total volume of tank, uses m3To indicate.
B: fermentation liquid puts the bioactivity total amount generated after tank, is generally indicated with hundred million u or 1,000,000,000 u.
Table 1
As shown in Table 1, two tanks pass through 164 hours fermenting and producings, and personnel survey sugar and surveyed with the sugared two ways of online survey The sugared content value obtained is very close.Full-automatic feed supplement under the conditions of online survey sugar saves culture than the feed supplement under the conditions of manually surveying sugar Base 3250L, the bioactivity total amount that final " manually and online " fermentation generates are respectively as follows: 21459.88 hundred million u and 21604.71 hundred million U, it can be clearly seen that the online automatic feeding surveyed under sugar guidance is more advantageous to the growth and metabolism of microorganism.
Table 2
As shown in Table 2, two tanks pass through 162 hours fermenting and producings, and personnel survey sugar and surveyed with the sugared two ways of online survey The sugared content value obtained is very close.Full-automatic feed supplement under the conditions of online survey sugar saves culture than the feed supplement under the conditions of manually surveying sugar Base 5320L, the bioactivity total amount that final " manually and online " fermentation generates are respectively as follows: 23602.10 hundred million u and 23799.53 hundred million U, it can be clearly seen that the online automatic feeding surveyed under sugar guidance is more advantageous to the growth and metabolism of microorganism.
Table 3
As shown in Table 3, two tanks pass through 162 hours fermenting and producings, and personnel survey sugar and surveyed with the sugared two ways of online survey The sugared content value obtained is very close.Full-automatic feed supplement under the conditions of online survey sugar saves culture than the feed supplement under the conditions of manually surveying sugar Base 3125L, the bioactivity total amount that final " manually and online " fermentation generates are respectively as follows: 26180.37 hundred million u and 26784.58 hundred million U, it can be clearly seen that the online automatic feeding surveyed under sugar guidance is more advantageous to the growth and metabolism of microorganism.
In conclusion utilizing the refractometer for including refractometer inductive probe in the utility model in terramycin fermentation process Carry out it is online survey sugar, can not only guarantee the instantaneity of detection and the normalization of fermentation process, and the result that detects and artificial The result for surveying sugar is very close, can be realized accurate feed supplement, makes the growth of microorganism and metabolism synchronous with nutrition supply, improves object Expect utilization rate, is more advantageous to the growth and metabolism of microorganism.Simultaneously, moreover it is possible to reduce personnel labor intensity and reduce because of repeatedly sampling Caused by fermentation liquid waste.
Finally, it is stated that preferred embodiment above is merely intended for describing the technical solutions of the present application, but not for limiting the present application, to the greatest extent Pipe has been described in detail the utility model by above preferred embodiment, but those skilled in the art should manage Solution, can make various changes, without departing from the utility model claims book institute to it in the form and details The range of restriction.

Claims (7)

1. a kind of refractometer inductive probe, which is characterized in that the inductive probe include detecting electrode (1), filter device (2), Air nozzle (3) and steam jet (4);The filter device (2) has cavity (5), and filtering knot is provided on cavity (5) outer wall Structure (6), filter device (2) are set in the front end of the detecting electrode (1);Air nozzle (3) setting is filled in the filtering It sets and filters the detection liquid for entering the cavity through the filter structure for input air on (2) to remove;The steam jet (4) it is arranged on the filter device (2) for inputting steam to clean the detection end of the detecting electrode (1).
2. a kind of refractometer inductive probe according to claim 1, which is characterized in that the cavity (5) by lid (7) and It is constituted with the ontology (8) of lid (7) cooperation, the detecting electrode (1), air nozzle (3) and steam jet (4) are arranged at institute It states on lid (7), the filter structure (6) is arranged on the ontology (8).
3. a kind of refractometer inductive probe according to claim 2, which is characterized in that the ontology (8) includes filtering folder It covers (9) and spacer screen pressure ring (10), the filtering collet (9) includes side and bottom surface, the filter structure (6) is separately positioned on institute It states on side and bottom surface;The side is with apertured double-layer structure and interval hollow out, and the gap is for being fixed at Filter structure (6) on the side;The bottom surface hollow out is fixed on the bottom surface by the spacer screen pressure ring (10) Filter structure (6).
4. a kind of refractometer inductive probe according to claim 1-3, which is characterized in that the filter device It (2) is in cylindrical body.
5. a kind of refractometer inductive probe according to claim 4, which is characterized in that the filter structure (6) is stainless Steel mesh.
6. a kind of refractometer inductive probe according to claim 4, which is characterized in that the air nozzle (3) and steam Spray head (4) is arranged around the detecting electrode (1), and is located on different circumference, and the quantity of the air nozzle (3) is greater than etc. In 2;The quantity of the steam jet (4) is more than or equal to 1.
7. a kind of refractometer inductive probe according to claim 4, which is characterized in that the air nozzle (3) and steam Spray head (4) is arranged around the detecting electrode (1), and is located on same circumference, and the quantity of the air nozzle (3) is greater than etc. In 2;The quantity of the steam jet (4) is more than or equal to 1.
CN201821296238.3U 2018-08-13 2018-08-13 A kind of refractometer inductive probe Active CN208505894U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108956532A (en) * 2018-08-13 2018-12-07 金河生物科技股份有限公司 A kind of refractometer inductive probe and its application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108956532A (en) * 2018-08-13 2018-12-07 金河生物科技股份有限公司 A kind of refractometer inductive probe and its application
CN108956532B (en) * 2018-08-13 2024-04-05 金河生物科技股份有限公司 Refractometer inductive probe and application thereof

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