CN108956532A - A kind of refractometer inductive probe and its application - Google Patents
A kind of refractometer inductive probe and its application Download PDFInfo
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- CN108956532A CN108956532A CN201810914713.7A CN201810914713A CN108956532A CN 108956532 A CN108956532 A CN 108956532A CN 201810914713 A CN201810914713 A CN 201810914713A CN 108956532 A CN108956532 A CN 108956532A
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- detecting electrode
- air nozzle
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- 239000000523 sample Substances 0.000 title claims abstract description 40
- 230000001939 inductive effect Effects 0.000 title claims abstract description 37
- 238000000855 fermentation Methods 0.000 claims abstract description 44
- 230000004151 fermentation Effects 0.000 claims abstract description 44
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 238000001914 filtration Methods 0.000 claims description 9
- KIPLYOUQVMMOHB-MXWBXKMOSA-L [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O Chemical compound [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O KIPLYOUQVMMOHB-MXWBXKMOSA-L 0.000 claims description 6
- 229940063650 terramycin Drugs 0.000 claims description 6
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims 1
- 239000010935 stainless steel Substances 0.000 claims 1
- 239000006052 feed supplement Substances 0.000 abstract description 17
- 238000000034 method Methods 0.000 abstract description 12
- 239000000463 material Substances 0.000 abstract description 6
- 238000005070 sampling Methods 0.000 abstract description 5
- 238000010992 reflux Methods 0.000 abstract description 4
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 238000010606 normalization Methods 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 6
- 230000004060 metabolic process Effects 0.000 description 6
- 239000012029 Fehling's reagent Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000004448 titration Methods 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 2
- 235000019345 sodium thiosulphate Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 201000004384 Alopecia Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 201000002451 Overnutrition Diseases 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- LZGCHNNAWUTUOV-UHFFFAOYSA-N anthracene copper Chemical compound [Cu].C1=CC=CC2=CC3=CC=CC=C3C=C12 LZGCHNNAWUTUOV-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 208000024963 hair loss Diseases 0.000 description 1
- 230000003676 hair loss Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- ICIWUVCWSCSTAQ-UHFFFAOYSA-M iodate Chemical compound [O-]I(=O)=O ICIWUVCWSCSTAQ-UHFFFAOYSA-M 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 235000020823 overnutrition Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- -1 potassium ferricyanide Chemical compound 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 108700002783 roundabout Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000003519 ventilatory effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of refractometer inductive probe and its applications, belong to quick detection device technical field, which includes detecting electrode；Filter device, the filter device have cavity, and surround and be provided with filter structure on each face of the cavity, and filter device is set in the front end of the detecting electrode；Air nozzle, the air nozzle, which is arranged on the filter device, filters the detection liquid for entering the cavity through the filter structure for input air to remove；Steam jet, the steam jet are arranged on the filter device for inputting steam to clean the detection end of the detecting electrode.Utilize the refractometer inductive probe; it can be realized filtering-sampling-detection-reflux automation; guarantee the instantaneity of detection and the normalization of fermentation process; accurate, real-time on-line monitoring is accomplished to fermentation liquid whole process; and then online feed supplement is precisely instructed; personnel labor intensity is reduced, reducing leads to the waste of material because not stopping sampling.
Description
Technical field
The invention belongs to quick detection device technical fields, and in particular to a kind of refractometer inductive probe and its application.
Background technique
It is well known that, as microorganism constantly breeds, energy source (culture medium) is also in microbial fermentation production process
It is consumed constantly, if supplemented not in time, the growth and breeding of microorganism will slow down or stagnate, but be not that feed supplement is mended more
It is much better, it because feed supplement is too many, will cause overnutrition, will affect the normal breeding and metabolism of microorganism instead.Culture medium needs
How much supplement is just to know to train in fermentor according to the instant sugared content value in the fermentation liquid measured using sugared content as foundation
The amount that base is short of is supported, fills into required culture medium in time by feeding-system.The standard of feed supplement amount will be directly affected due to surveying sugar
True property, and feed supplement influences the quality of fermentation liquid, so sugared content value becomes one for fermentation success or failure in accurate detection fermentation liquid
A key point.
China's fermentation industry occupies an important position in national economy, but overall technology level is compared with developed countries also
Biggish gap, that there are raw material availabilities and conversion ratio is low, energy consumption is high, the pollution problems such as again.It is mould in soil both at home and abroad at present
It is still after using traditional timing sampling with potassium ferricyanide method, anthracene copper ratio that methods and techniques used in sugar are surveyed in plain fermentation process
Color method or fehling reagent titration.Wherein, fehling reagent titration specifically: polysaccharide generates glucose, grape after sour water solution
It can be by the Cu in fehling reagent under aldehyde radical alkaline condition in sugar2+It restores and generates copper oxidule precipitation, it is remaining in fehling reagent
Cu2+Iodine is precipitated with excessive iodate nak response in acid condition, iodine can use sodium thiosulfate standard solution direct titration, according to sulphur
The dosage of sodium thiosulfate can find out the content of total reducing sugar in sample indirectly.However this round-about way seems time-consuming and laborious, obtains
Data also can only as " out-of-date reference " cannot in time, effectively, fermentation liquid sugar amount value in true reflection tank, because according to
It is to need the regular hour that the numerical value measured in this way, which calculates the amount that culture medium in fermentor requires supplementation with, so calculating at this time
Feed supplement amount be not the amount really needed in tank in fact, this always slow half operation clapped undoubtedly will affect fermenting and producing
Quality and yield, also affect entire fermentation industry fermentation level certainly.
Intermittent sample detection exists as following drawbacks: (1) large labor intensity of personnel；(2) continuous sampling causes to expect
The waste of liquid with；(3) the sugar amount value that method measures according to this instructs feed supplement accuracy poor.So being badly in need of a kind of faster more convenient and fast method
It realizes in fermentation process to the on-line monitoring of sugar.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of refractometer inductive probes；The second purpose is to provide one kind
Refractometer containing the refractometer inductive probe；The third purpose is to provide the refractometer inductive probe during the fermentation
Realize the application in on-line checking.
In order to achieve the above objectives, the invention provides the following technical scheme:
1, a kind of refractometer inductive probe, the inductive probe include detecting electrode 1, filter device 2,3 and of air nozzle
Steam jet 4；The filter device 2 has cavity 5, is provided with filter structure 6 on 5 outer wall of cavity, filter device 2 is set in institute
State the front end of detecting electrode 1；The air nozzle 3 is arranged on the filter device 2 for input air to remove described in warp
Filter structure filtering enters the detection liquid of the cavity；The steam jet 4 is arranged on the filter device 2 to be steamed for inputting
Vapour is to clean the detection end of the detecting electrode 1.
Preferably, the cavity 5 is constituted from lid 7 and with the ontology 8 with lid 7, the detecting electrode 1, air spray
Mouth 3 and steam jet 4 are arranged on the lid 7, and the filter structure 6 is arranged on the ontology 8.
Preferably, the ontology 8 includes filtering collet 9 and spacer screen pressure ring 10, and the filtering collet 9 includes side and bottom
Face, the filter structure 6 are separately positioned on the side and bottom surface；The side is with apertured double-layer structure and interval
Hollow out, the gap are used for the filter structure 6 being fixed on the side；The bottom surface hollow out passes through the spacer screen pressure
Ring 10 is fixed at the filter structure 6 on the bottom surface.
Preferably, the filter device 2 is in cylindrical body.
Preferably, the filter structure 6 is stainless (steel) wire.
Preferably, the air nozzle 3 and steam jet 4 are arranged around the detecting electrode 1, and are located at different circumference
On, the quantity of the air nozzle 3 is more than or equal to 2；The quantity of the steam jet 4 is more than or equal to 1.
Preferably, the air nozzle 3 and steam jet 4 are arranged around the detecting electrode 1, and are located at same circumference
On, the quantity of the air nozzle 3 is more than or equal to 2；The quantity of the steam jet 4 is more than or equal to 1.
2, the refractometer comprising a kind of refractometer inductive probe.
3, a kind of refractometer inductive probe realizes the application in on-line checking during the fermentation.
Preferably, realize that on-line checking is specially to realize online survey sugar in terramycin fermentation process in the fermentation process.
Preferably, realize that on-line checking is specially to realize online survey sugar in terramycin fermentation process in the fermentation process.
The beneficial effects of the present invention are: the present invention provides a kind of refractometer inductive probe and its applications, by existing
There is refractometer inductive probe housing setting tool to have the filter device for being provided with filter structure on cavity and outer wall, it is ensured that detecting
It can be effectively filtered the thick substances in hair loss zymotic fluid in the process, contact inductive probe with clear detection liquid, Jin Erti
High detection precision.Meanwhile air nozzle is set on filter device, generated pressure when by input filtrated air, with
It removes and enters the detection liquid of cavity through filtering structured filter, and then achieve the purpose that on-line real-time measuremen, in addition, filter device is also
It is provided with steam jet, steam is inputted to clean the detection end of detecting electrode by the spray head, is further ensured that detection is accurate
Degree.In addition, on the one hand can guarantee uniform force inside and outside the inductive probe, another aspect energy when filter device is in cylindrical body
The flow resistance for enough reducing fermentation liquid in fermentor, keeps its flowing more smooth, not will form local turbulence, last cylindrical body is smooth
Appearance be easier clean, disinfection do not stay dead angle.Using the refractometer inductive probe, filtering-sampling-detection-can be realized
The automation of reflux guarantees the instantaneity of detection and the normalization of fermentation process, to fermentation liquid whole process accomplish it is accurate, in real time exist
Line monitoring, and then online feed supplement is precisely instructed, personnel labor intensity is reduced, reducing leads to the wave of material because not stopping sampling
Take.
Detailed description of the invention
In order to keep the purpose of the present invention, technical scheme and beneficial effects clearer, the present invention provides following attached drawing and carries out
Illustrate:
Fig. 1 is refractometer inductive probe schematic diagram in the present invention；
Fig. 2 is refractometer inductive probe perspective view in invention；
Fig. 3 is refractometer inductive probe top view in the present invention (omitting lid).
Fig. 1 is into Fig. 3,1- detecting electrode；2- filter device；3- air nozzle；4- steam jet；5- cavity；6- filtering
Structure；7- lid；8- ontology；9- filters collet；10- spacer screen pressure ring.
Specific embodiment
Below by a preferred embodiment of the present invention will be described in detail.
As illustrated in the accompanying drawings from 1 to 3, the element numbers in attached drawing respectively indicate refractometer inductive probe used in embodiment:
1- detecting electrode；2- filter device；3- air nozzle；4- steam jet；5- cavity；6- filter structure；7- lid；8- ontology；
9- filters collet；10- spacer screen pressure ring.
In the present invention refractometer inductive probe using when be dipped in fermentation liquid completely, due in fermentation process, in fermentor
It is a kind of mixing state under stirring system work from beginning to end, so when the fermentation broth stream at a certain moment is through filter device 2,
By the filter structure 6 being arranged thereon, material moment has carried out solid- liquid separation, clear to enter filter device 2 to liquid
Cavity 5 in, sticky material is trapped within outer, and simultaneously detecting electrode 1 is sampled, after the completion of sample measurement, cavity 5
In the filtrated air that is inputted by air nozzle 3 of detection liquid blow out, meanwhile, steam jet 4 to the detection end of detecting electrode 1 into
Row cleaning, cleaning solution are blown out by filtrated air together with detection liquid, that is, complete the detection to that moment fermentation liquid, filtrate reflux
After be again and then filtering-sampling-detection-reflux, so circulation carries out above step, continuous to complete a system
Column detection operation.
Embodiment
Refractometer inductive probe in the present invention is used for terramycin fermentation process and surveys sugar online, realizes accurate feed supplement
It chooses one group of fermentor (one group includes three fermentors), installation kit contains refractometer sense in the present invention respectively on tank
The refractometer that should be popped one's head in, and refractometer and feeding-system are subjected to data linkage, feed back to feeding-system according to refractometer
Data correspondingly provide optimal culture medium and wait for benefit amount and add in fermentor, the index of refraction of the refractometer be nD ±
0.0002, the operating temperature of optical core components is -20-150 DEG C in refractometer inductive probe, and filter structure is in inductive probe
304 stainless (steel) wires of 150 mesh of aperture, inductive probe is protruded into tank, and rest part is isolated in outer by tank skin.
In addition one group of fermentor (one group include three fermentors) is taken again, refractometer is not installed, and during later stage fermentation, is led to
The mode for crossing artificial detection detects sugared content in fermentation liquid.
Above-mentioned two groups of fermentors are used for the terramycin fermenting and producing of three groups of six batches, specifically: it is prepared in fermentor
After culture medium needed for production, " disappearing in fact " sterilization treatment is done to entire tank, when tank temperature drops to technique required value, is moved from seeding tank
To fermentor, fermentation starts kind, and two groups of fermentors remove whether installing different on refractometer, other all structures and specification additional
All the same, parameter of materials used is the same in two groups of fermentors, and it is same with batch for accessing production strain used in two groups of fermentors
One strain, and access amount is identical, and the control parameters such as two groups of fermentor production cycles, ventilatory capacity, pH value are all the same.Fermentation process
In compile related data, the results are shown in Table 1 to 3, carry out bioactivity calculating, and the sugared, feed supplements to two kinds of survey using formula (1)
Method controls lower fermentation level and compares.
In formula:
A: fermentation liquid puts tank unit (that is: potency), is indicated with u/ml.
V: fermentation liquid puts the total volume of tank, uses m3To indicate.
B: fermentation liquid puts the bioactivity total amount generated after tank, is generally indicated with hundred million u or 1,000,000,000 u.
Table 1
As shown in Table 1, two tanks pass through 164 hours fermenting and producings, and personnel survey sugar and surveyed with the sugared two ways of online survey
The sugared content value obtained is very close.Full-automatic feed supplement under the conditions of online survey sugar saves culture than the feed supplement under the conditions of manually surveying sugar
Base 3250L, the bioactivity total amount that final " manually and online " fermentation generates are respectively as follows: 21459.88 hundred million u and 21604.71 hundred million
U, it can be clearly seen that the online automatic feeding surveyed under sugar guidance is more advantageous to the growth and metabolism of microorganism.
Table 2
As shown in Table 2, two tanks pass through 162 hours fermenting and producings, and personnel survey sugar and surveyed with the sugared two ways of online survey
The sugared content value obtained is very close.Full-automatic feed supplement under the conditions of online survey sugar saves culture than the feed supplement under the conditions of manually surveying sugar
Base 5320L, the bioactivity total amount that final " manually and online " fermentation generates are respectively as follows: 23602.10 hundred million u and 23799.53 hundred million
U, it can be clearly seen that the online automatic feeding surveyed under sugar guidance is more advantageous to the growth and metabolism of microorganism.
Table 3
As shown in Table 3, two tanks pass through 162 hours fermenting and producings, and personnel survey sugar and surveyed with the sugared two ways of online survey
The sugared content value obtained is very close.Full-automatic feed supplement under the conditions of online survey sugar saves culture than the feed supplement under the conditions of manually surveying sugar
Base 3125L, the bioactivity total amount that final " manually and online " fermentation generates are respectively as follows: 26180.37 hundred million u and 26784.58 hundred million
U, it can be clearly seen that the online automatic feeding surveyed under sugar guidance is more advantageous to the growth and metabolism of microorganism.
In conclusion being carried out in terramycin fermentation process using the refractometer for including refractometer inductive probe in the present invention
Sugar is surveyed online, can not only guarantee the instantaneity of detection and the normalization of fermentation process, and the result detected surveys sugar with artificial
Result it is very close, can be realized accurate feed supplement, make the growth of microorganism and metabolism synchronous with nutrition supply, raising material is sharp
With rate, it is more advantageous to the growth and metabolism of microorganism.Simultaneously, moreover it is possible to caused by reducing personnel labor intensity and reducing because repeatedly sampling
Fermentation liquid waste.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (10)
1. a kind of refractometer inductive probe, which is characterized in that the inductive probe include detecting electrode (1), filter device (2),
Air nozzle (3) and steam jet (4)；The filter device (2) has cavity (5), and filtering knot is provided on cavity (5) outer wall
Structure (6), filter device (2) are set in the front end of the detecting electrode (1)；Air nozzle (3) setting is filled in the filtering
It sets and filters the detection liquid for entering the cavity through the filter structure for input air on (2) to remove；The steam jet
(4) it is arranged on the filter device (2) for inputting steam to clean the detection end of the detecting electrode (1).
2. a kind of refractometer inductive probe according to claim 1, which is characterized in that the cavity (5) by lid (7) and
It is constituted with the ontology (8) of lid (7) cooperation, the detecting electrode (1), air nozzle (3) and steam jet (4) are arranged at institute
It states on lid (7), the filter structure (6) is arranged on the ontology (8).
3. a kind of refractometer inductive probe according to claim 2, which is characterized in that the ontology (8) includes filtering folder
It covers (9) and spacer screen pressure ring (10), the filtering collet (9) includes side and bottom surface, the filter structure (6) is separately positioned on institute
It states on side and bottom surface；The side is with apertured double-layer structure and interval hollow out, and the gap is for being fixed at
Filter structure (6) on the side；The bottom surface hollow out is fixed on the bottom surface by the spacer screen pressure ring (10)
Filter structure (6).
4. a kind of refractometer inductive probe according to claim 1-3, which is characterized in that the filter device
It (2) is in cylindrical body.
5. a kind of refractometer inductive probe according to claim 4, which is characterized in that the filter structure (6) is stainless
Steel mesh.
6. a kind of refractometer inductive probe according to claim 4, which is characterized in that the air nozzle (3) and steam
Spray head (4) is arranged around the detecting electrode (1), and is located on different circumference, and the quantity of the air nozzle (3) is greater than etc.
In 2；The quantity of the steam jet (4) is more than or equal to 1.
7. a kind of refractometer inductive probe according to claim 4, which is characterized in that the air nozzle (3) and steam
Spray head (4) is arranged around the detecting electrode (1), and is located on same circumference, and the quantity of the air nozzle (3) is greater than etc.
In 2；The quantity of the steam jet (4) is more than or equal to 1.
8. including a kind of described in any item refractometers of refractometer inductive probe of claim 1-7.
9. a kind of described in any item refractometer inductive probes of claim 1-7 realize answering in on-line checking during the fermentation
With.
10. application according to claim 9, which is characterized in that realize that on-line checking specially exists in the fermentation process
Terramycin fermentation process realizes online survey sugar.
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