CN109266540A - A kind of bioreactor and its application method based on simulation of climatic data natural conditions - Google Patents
A kind of bioreactor and its application method based on simulation of climatic data natural conditions Download PDFInfo
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- CN109266540A CN109266540A CN201811168900.1A CN201811168900A CN109266540A CN 109266540 A CN109266540 A CN 109266540A CN 201811168900 A CN201811168900 A CN 201811168900A CN 109266540 A CN109266540 A CN 109266540A
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of bioreactor and its application method based on simulation of climatic data natural conditions, cylindrical reactor ontology including being equipped with top cover and bottom plate, the top cover is equipped with feed inlet, and the feed inlet is connected with peristaltic pump and sample introduction tank by silicone tube；The bottom plate is equipped with strip-shaped tube lamp, the strip-shaped tube lamp is electrically connected with controller, temperature controller is additionally provided on the bottom plate, the temperature controller is electrically connected by temperature control line and the controller, the bottom plate is equipped with monitoring probe, the monitoring probe is electrically connected by monitoring line and data repository, and the data repository and the controller are electrically connected；Gas distributor there are two being set on the bottom plate, the gas distributor is connected with humidifying pot by tracheae, the humidifying pot is connected separately with carbon dioxide tank and air tank by two tracheaes, present apparatus accurate reproduction natural climate condition can expand the algae bio amount productive potentialities of culture based on meteorological data Fast Evaluation somewhere algae.
Description
Technical field
The present invention relates to photobioreactor fields, and in particular to a kind of photoproduction based on simulation of climatic data natural conditions
Object reactor.
Background technique
Algae has huge application value in nature ecology and production process, can be used for sewage treatment, health care doctor
The fields such as medicine and bio-fuel research and development.Currently, algae culture situation is more apparent in the lab scale of laboratory, still, in algae
During extensive pilot scale culture is expanded in natural environment from the lab scale culture in laboratory be still faced with following problems:
1) illumination in natural environment is dynamic change, and diurnal variation and seasonal variations can all cause intensity of illumination to generate
Change, influences the accumulation of algal grown and biomass；
2) determine that another factor of algae culture is temperature, the variation of temperature will produce algal grown situation in natural environment
It is raw to influence.
The yield for cultivating algae under a certain weather conditions in a certain region in actual environment how is quickly evaluated, urgent need is become
It solves the problems, such as.In response to the above problems, there are mainly two types of technical solutions at present:
1) based on outdoor culture apparatus, pass through the culture or one in a season under a certain region or a certain weather conditions
The yield that algae is cultivated in the environment is evaluated in the culture in a year with actual biomass, and this method evaluation result is reliable, still
There are evaluation times it is long, capital consumption is more the problems such as.
2) the typical weather period is selected, the yield of algae bio amount under different typical weather conditions is measured, and then is calculated
The biomass yield of algae under the annual weather conditions, this method is lower relative to first method capital investment, but due to certainly
Climate change is larger under right weather conditions, and selecting representative experimental stage weather conditions is also a great problem.
Therefore, it is necessary to construct a kind of bioreactor that can reappear illumination and temperature condition under natural situation in high precision,
And it is based on above-mentioned reactor, a kind of cultural method is introduced, the culture situation of algae under Fast Evaluation physical condition, to assess the ground
The potentiality of algae production.
Summary of the invention
For above-mentioned technical deficiency, the object of the present invention is to provide the light that one kind can quickly adjust culture apparatus
According to temperature condition, and the meteorological data based on somewhere adjusts the illumination and temperature of reactor, to reappear and simulate the gas in somewhere
Time condition further studies the culture situation of algae under the physical condition.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
The present invention provides a kind of bioreactor based on simulation of climatic data natural conditions, including be provided with top cover and
The cylindrical reactor ontology of bottom plate is provided with feed inlet on the top cover, and the feed inlet is connected with wriggling by silicone tube
Pump, the peristaltic pump are connected with sample introduction tank by the silicone tube；The bottom plate upper surface is provided centrally with strip-shaped tube lamp, institute
It states strip-shaped tube lamp and controller is electrically connected with by light-operated line, temperature controller, the temperature control are additionally provided on the bottom plate upper surface
Device is electrically connected by temperature control line and the controller, is additionally provided with monitoring probe on the bottom plate, the monitoring probe passes through
It monitors line and data repository is electrically connected, the data storage is electrically connected with the controller；It is also set up on the bottom plate
There are two gas distributor, the gas distributor is also connected with humidifying pot by tracheae, passes through two gas on the humidifying pot
Pipe is separately connected there are two flowmeter, and the flowmeter is connected with valve by the tracheae, one of them described valve passes through
The tracheae is connected with carbon dioxide tank, another described valve is connected with air tank by the tracheae.
Preferably, air valve is additionally provided on the top cover.
Preferably, there are two blenders for the bottom surface setting of the top cover.
Preferably, sample tap is additionally provided on the reactor body.
Preferably, the reactor body is made of transparent organic glass, whole cylindrical, the capping and the bottom
Plate matter is PVC, and black spongy layer is enclosed in the peripheral outer wall of the reactor body, sea is arranged on the black spongy layer
Silk floss lid.
Preferably, polychloroethylene film is enclosed on the strip-shaped tube lamp.
The beneficial effects of the present invention are: the illumination and temperature condition in bioreactor can be quickly adjusted, is realized
High-precision reappears the possibility of natural climate condition, can expand the algae bio of culture based on meteorological data Fast Evaluation somewhere algae
Productive potentialities are measured, the time loss and fund for removing pilot experiment from are paid.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is a kind of knot of the bioreactor based on simulation of climatic data natural conditions provided in an embodiment of the present invention
Structure schematic diagram；
Fig. 2 is the structural schematic diagram of top cover provided in an embodiment of the present invention；
Fig. 3 is the structural schematic diagram of bottom plate provided in an embodiment of the present invention；
Fig. 4 is the connection of reactor body provided in an embodiment of the present invention, blender, strip-shaped tube lamp and gas distributor
Schematic diagram.
Description of symbols:
1, sample introduction tank；2, peristaltic pump；3, feed inlet；4, air valve；5, reactor body；6, sample tap；7, temperature control line；8, light
Control line；9, line is monitored；10, humidifier；11, flowmeter；12, valve；13, controller；14, repository；15, blender；16, it supervises
Probing head；17, temperature controller；18, strip-shaped tube lamp；19, gas distributor.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figures 1 to 4, a kind of bioreactor based on simulation of climatic data natural conditions, including be provided with
The cylindrical reactor ontology 5 of top cover and bottom plate, feed inlet 3 is provided on the top cover, and the feed inlet 3 passes through silicone tube and connects
It is connected to peristaltic pump 2, the peristaltic pump 2 is connected with sample introduction tank 1 by the silicone tube；The bottom plate upper surface is provided centrally with
The intensity of light source of strip-shaped tube lamp 18, the strip-shaped tube lamp 18 is adjusted according to meteorological data by control system, the strip-shaped tube lamp 18
It is electrically connected with controller 13 by light-operated line 8, temperature controller 17 is additionally provided on the bottom plate upper surface, the temperature controller 17 is logical
It crosses temperature control line 7 and the controller 13 is electrically connected, the actual temp in the reactor body 5 is according to meteorological data by controlling
System is adjusted, and monitoring probe 16 is additionally provided on the bottom plate, and the monitoring probe 16 passes through monitoring line 9 and data repository 14
It is electrically connected, the data repository 14 is electrically connected with the controller 13, can prestore number according to the data repository 14
According to the temperature and intensity of illumination regulated and controled in the reactor body 5, also set up that there are two gas distributor 19, institutes on the bottom plate
It states gas distributor 19 and humidifying pot 10 is also connected with by tracheae, be separately connected on the humidifying pot 10 by two tracheaes
There are two flowmeter 11, the flowmeter 11 is connected with valve 12 by the tracheae, one of them described valve 12 passes through institute
It states tracheae and is connected with carbon dioxide tank, another described valve 12 is connected with air tank by the tracheae.
It is additionally provided with air valve on the top cover, the air pressure inside of the reactor body 5 is adjusted.
There are two blenders 15 for the bottom surface setting of the top cover, when gas distributor is aerated insufficient, as spare stirring
Device guarantees carbon dioxide required for algal grown.
Be additionally provided with sample tap 6 on the reactor body 5, can direct sample be measured to analyze algal grown shape
Condition.
The reactor body 5 is made of transparent organic glass, whole cylindrical, the capping and the bottom plate material
For PVC, it is enclosed with black spongy layer in the peripheral outer wall of the reactor body 5, is provided with sponge on the black spongy layer
Lid, the black spongy layer constituted with the top cover that is made by opaque material with the bottom plate be completely closed it is lighttight
Reaction column avoids influence of the external uncontrollable strip-shaped tube lamp to reactor.
Polychloroethylene film is enclosed on the strip-shaped tube lamp 18.
In use, peristaltic pump 2 adjusts bar shaped by the culture medium sucting reaction device main body 5 in sample introduction tank 1, using controller 13
18 intensity of light source of spot adjusts temperature controller 17 using controller 13 to adjust temperature in reactor body 5, mix up temperature with
Illumination condition guarantees algal grown in order, and carbon dioxide and air are passed through humidifying pot 10, flowmeter 11, valve 12, gas
Body distributor 19 enters in reactor body 5, and when gas distributor 19 is aerated deficiency, blender 15 is used as spare agitating device
It is stirred to react 5 inside culture medium of device main body, guarantees carbon dioxide required for algal grown, different regions meteorological data can
It is stored by data storage bank 14 and is connected to controller 13 and further adjust inside reactor environmental condition, to restore somewhere reality
Border weather conditions, altitude simulation outdoor conditions while, can also be achieved the automatic adjustment of system, the prison in real time of on-line monitoring probe 16
5 inside algal grown condition of reactor body is controlled, data storage bank 14 is sent data to by monitoring line 9, to store data
Analyze algal grown situation, air valve 4 is adjusted 5 air pressure inside of reactor body, sample tap 6 can direct sample be measured with point
Analyse algal grown situation.
The invention has the advantages that the illumination and temperature condition in bioreactor can be adjusted quickly, realize that high-precision is reappeared
The possibility of natural climate condition, the algae bio amount production that can expand culture based on meteorological data Fast Evaluation somewhere algae are latent
Power, the time loss and fund for removing pilot experiment from are paid.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (7)
1. a kind of bioreactor based on simulation of climatic data natural conditions, it is characterised in that: including be provided with top cover and
The cylindrical reactor ontology of bottom plate is provided with feed inlet on the top cover, and the feed inlet is connected with wriggling by silicone tube
Pump, the peristaltic pump are connected with sample introduction tank by the silicone tube；The bottom plate upper surface is provided centrally with strip-shaped tube lamp, institute
It states strip-shaped tube lamp and controller is electrically connected with by light-operated line, temperature controller, the temperature control are additionally provided on the bottom plate upper surface
Device is electrically connected by temperature control line and the controller, is additionally provided with monitoring probe on the bottom plate, the monitoring probe passes through
It monitors line and data repository is electrically connected, the data repository and the controller are electrically connected；It is also set on the bottom plate
It sets there are two gas distributor, the gas distributor is also connected with humidifying pot by tracheae, pass through two on the humidifying pot
Tracheae is connected separately with two flowmeters, and the flowmeter is connected with valve by the tracheae, one of them described valve is logical
It crosses the tracheae and is connected with carbon dioxide tank, another described valve is connected with air tank by the tracheae.
2. a kind of bioreactor based on simulation of climatic data natural conditions as claimed in claim, it is characterised in that:
Air valve is additionally provided on the top cover.
3. a kind of bioreactor based on simulation of climatic data natural conditions as claimed in claim, it is characterised in that:
There are two blenders for the bottom surface setting of the top cover.
4. a kind of bioreactor based on simulation of climatic data natural conditions as claimed in claim, it is characterised in that:
Sample tap is additionally provided on the reactor body.
5. a kind of bioreactor based on simulation of climatic data natural conditions as claimed in claim, it is characterised in that:
The reactor body is made of transparent organic glass, whole cylindrical, and the capping is PVC, institute with the bottom plate material
It states and is enclosed with black spongy layer in the peripheral outer wall of reactor body, be provided with the sponge that can cover conjunction on the black spongy layer
Lid.
6. a kind of bioreactor based on simulation of climatic data natural conditions as claimed in claim, it is characterised in that:
Polychloroethylene film is enclosed on the strip-shaped tube lamp.
7. a kind of application method of the bioreactor based on simulation of climatic data natural conditions: will be in sample introduction tank by peristaltic pump
Culture medium sucting reaction device main body, using controller adjust the strip-shaped tube lamp intensity of light source, using controller adjust temperature controller from
And adjust the temperature in reactor body, mix up temperature and illumination condition, guarantee algal grown in order, by carbon dioxide with
Air enters in reactor body by humidifying pot, flowmeter, valve, gas distributor, when gas distributor is aerated insufficient,
Blender is stirred to react device body interior culture medium as spare agitating device, guarantees titanium dioxide required for algal grown
Carbon, different regions meteorological data, which can be stored by data storage bank and be connected to controller, further adjusts inside reactor environment
Condition, to restore the practical weather conditions in somewhere, altitude simulation outdoor conditions while, can also be achieved the automatic adjustment of system, online
Monitoring probe monitors algal grown condition inside reactor body in real time, sends data to data storage bank by monitoring line,
To store data analysis algal grown situation, reactor body air pressure inside is adjusted in air valve, and sample tap can direct sample progress
Measurement is to analyze algal grown situation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811168900.1A CN109266540A (en) | 2018-10-08 | 2018-10-08 | A kind of bioreactor and its application method based on simulation of climatic data natural conditions |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811168900.1A CN109266540A (en) | 2018-10-08 | 2018-10-08 | A kind of bioreactor and its application method based on simulation of climatic data natural conditions |
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| CN109266540A true CN109266540A (en) | 2019-01-25 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350494A (en) * | 2022-01-14 | 2022-04-15 | 南京信息工程大学 | Multi-parameter intelligent microorganism culture system under climate change condition |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1696275A (en) * | 2005-05-26 | 2005-11-16 | 上海交通大学 | Culturing system of light bioreactor for online automatic supplying nutrient source for plant |
| CN101538533A (en) * | 2009-04-23 | 2009-09-23 | 浙江大学 | Wetland micro-ecological simulation method |
| CN201517107U (en) * | 2009-08-31 | 2010-06-30 | 环境保护部南京环境科学研究所 | Alga experiment analog device for controlling temperature, light and flow rate |
| CN202730113U (en) * | 2012-08-29 | 2013-02-13 | 江苏科技大学 | Microalgae high-density culture plant |
-
2018
- 2018-10-08 CN CN201811168900.1A patent/CN109266540A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1696275A (en) * | 2005-05-26 | 2005-11-16 | 上海交通大学 | Culturing system of light bioreactor for online automatic supplying nutrient source for plant |
| CN101538533A (en) * | 2009-04-23 | 2009-09-23 | 浙江大学 | Wetland micro-ecological simulation method |
| CN201517107U (en) * | 2009-08-31 | 2010-06-30 | 环境保护部南京环境科学研究所 | Alga experiment analog device for controlling temperature, light and flow rate |
| CN202730113U (en) * | 2012-08-29 | 2013-02-13 | 江苏科技大学 | Microalgae high-density culture plant |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350494A (en) * | 2022-01-14 | 2022-04-15 | 南京信息工程大学 | Multi-parameter intelligent microorganism culture system under climate change condition |
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