CN113063902A - Portable analysis system for field real-time determination of plant photosynthesis - Google Patents
Portable analysis system for field real-time determination of plant photosynthesis Download PDFInfo
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- 230000029553 photosynthesis Effects 0.000 title claims abstract description 45
- 238000010672 photosynthesis Methods 0.000 title claims abstract description 45
- 238000004458 analytical method Methods 0.000 title claims abstract description 13
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 204
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 107
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 102
- 238000010521 absorption reaction Methods 0.000 claims abstract description 20
- 238000003860 storage Methods 0.000 claims abstract description 12
- 239000000523 sample Substances 0.000 claims description 24
- 238000001514 detection method Methods 0.000 claims description 21
- 238000005259 measurement Methods 0.000 claims description 13
- 230000008859 change Effects 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 12
- 229910052751 metal Inorganic materials 0.000 claims description 12
- 239000002184 metal Substances 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 10
- 239000013307 optical fiber Substances 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- 238000003556 assay Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000006386 neutralization reaction Methods 0.000 claims description 2
- 230000009467 reduction Effects 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 238000002834 transmittance Methods 0.000 claims description 2
- 239000001117 sulphuric acid Substances 0.000 claims 1
- 235000011149 sulphuric acid Nutrition 0.000 claims 1
- 230000000243 photosynthetic effect Effects 0.000 abstract description 37
- 238000002474 experimental method Methods 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 31
- 238000000034 method Methods 0.000 description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 239000012528 membrane Substances 0.000 description 7
- 241000209094 Oryza Species 0.000 description 6
- 235000007164 Oryza sativa Nutrition 0.000 description 6
- 230000003139 buffering effect Effects 0.000 description 6
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- 238000012986 modification Methods 0.000 description 4
- 238000012937 correction Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
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- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000005431 greenhouse gas Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000036387 respiratory rate Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/004—CO or CO2
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Abstract
The invention relates to a portable analysis system for determining plant photosynthesis in real time in the field, which consists of an external circulating leaf chamber, a carbon dioxide absorption pool, a circulating pump, a carbon dioxide concentration detector, a carbon dioxide storage tank and a multi-angle fixing frame, wherein the external circulating leaf chamber is fixed on the multi-angle fixing frame, the carbon dioxide storage tank, the carbon dioxide absorption pool and the circulating pump are communicated with the external circulating leaf chamber through an organic hose and a valve, and the carbon dioxide concentration detector is arranged in the external circulating leaf chamber. The system can ensure that the concentration of carbon dioxide in the leaf chamber is completely the same, avoids the phenomenon that the concentration of carbon dioxide is uneven due to photosynthesis in the leaf chamber, provides feasibility for a photosynthetic intensity experiment for measuring the whole plant or a single leaf for a long time, and can effectively slow down the temperature rise of the closed environment of the leaf chamber and effectively maintain the stability of the environment humidity.
Description
Technical Field
Belongs to the technical field of plant photosynthesis detection, and particularly relates to a portable analysis system for determining plant photosynthesis in real time in the field.
Background
At present, Licor-6800, LCi-SD, CIRAS-3 and other portable photosynthesis instruments are available in the market, and provide a photosynthesis determination technical means for plant ecological research. This kind of photosynthetic apparatus comparison is applicable to the indoor control experiment, however, when the field experiment, because the admission line that is furnished with is not isolated with the external world, easily receive the interference of ground human breathing and ground activity to cause the inhomogeneous problem of business turn over gas, the different accuracy that directly influences plant photosynthesis of the different angles of growth of the locellus undersize of survey plant leaf and plant leaf in addition.
Chinese patent application 201310682081.3, the title of the invention "Whole plant tissue culture seedling photosynthetic rate rapid determination device" discloses a whole plant tissue culture seedling photosynthetic rate rapid determination device and method, including the IRGA sample room of the plant photosynthetic apparatus, the mounting plate of the leaf room of the soil, RGA sample room and mounting plate of the leaf room of the soil make up the complex, there is a transparent homemade leaf room in addition, there are fixed orifices, air vents and backflow union on the homemade leaf room; the self-made leaf chamber is connected with the soil leaf chamber mounting plate through a fixing hole, and the self-made leaf chamber is in rigid sealing connection with the complex; the RGA sample chamber is connected with the photosynthetic apparatus host computer through a data line. Although the invention improves the prior plant photosynthetic determinator, adopts the self-made leaf chamber to replace the standard leaf chamber, the photosynthetic apparatus can intuitively display the photosynthetic rate of the plant under the condition of no mechanical compression damage in real time, and can randomly determine the photosynthetic rate of a single plant or a whole bottle (group) of tissue culture seedlings; also solves the problem that the area is less than 6cm in the standard leaf chamber2The problem of single leaf, but the photosynthesis of single leaf of a certain plant cannot be measured.
Chinese patent application 201520814463.1, the title of the invention "a plant photosynthetic rate measuring device" discloses a plant photosynthetic rate measuring device, which comprises an LED lamp, a light source for providing plant light, and a light intensity adjusting device; air pump for delivering CO2A gas; a vane chamber for fixing the measuring vane; flow meter, detecting CO2The gas flow rate; CO 22Analyzer, detecting CO2Concentration; temperature and humidity sensor for detecting CO2The temperature and humidity of the gas change; the data acquisition unit is used for collecting and recording the acquired data; and the computer analyzes the collected data. But not control CO2Concentration and photosynthetic detection of leaves in different directions.
Chinese patent application 201610961666.2 entitled "photosynthetic determination error correction method based on physiological model under field conditions" discloses a method for measuring photosynthetic determination error in advanceMeasuring photosynthetic value A and atmospheric water vapor pressure difference DsTemperature value TK(ii) a Then respectively measuring ambient condition value atmospheric water vapor pressure difference value D in the lights standardTemperature value TK standardDifference value D from the atmospheric water vapor pressure value under the standard environmental condition to be calibrateds standardTemperature value TK standardRespectively substituting into the correction formula to obtain ADs,TKAnd with obtaining ADs Standard, TK StandardThen corrected to obtain ACalibration. The method can quantify the influence of environmental factors (such as temperature and humidity) on photosynthesis through the photosynthetic physiological model, thereby carrying out corresponding correction according to the change values of the temperature and the humidity in the photosynthetic determination process.
Chinese patent application 201721770487.7 entitled "device for measuring photosynthetic rate, respiration rate and greenhouse gas emission at different heights of crop population" discloses a device comprising a test box, a first support rod, a second support rod, a third support rod, 3N detection components and a signal collector, wherein the test box comprises a top cover, box and base, be equipped with first recess on the box top, the box wall mounting fan in, a pair of support frame is installed to the symmetry on the box inner wall, first branch, second branch, the both ends of third branch are fixed on two support frames through the detachably mode respectively, first branch, second branch, equidistant distribution on vertical direction of third branch, first branch, install a N determine module on second branch and the third branch respectively, the second recess that the base top was equipped with, each electrical components is connected with the signal collector electricity respectively. The method is based on the device, can measure the light intensity, the temperature and humidity, the photosynthetic rate, the respiratory rate and the emission of greenhouse gases at different heights of a crop group, but cannot measure the leaves of plants in different directions.
Chinese patent application 201711446350.0 entitled "device for extending temperature control range of photosynthesis determination system by circulating alcohol" discloses a device for controlling temperature range, which utilizes a circulating pump to deliver cooled or heated liquid alcohol, and sends the liquid alcohol to a metal part (containing a plurality of water channels for alcohol to flow through) tightly attached to an LI-6400XT photosynthesis measurement chamber through a PVC pipe, and changes the temperature of the metal part; the temperature of the photosynthesis measuring chamber is gradually adjusted through the heat exchange process, and finally, the temperature control range of the portable photosynthesis measuring system (LI-6400XT) is effectively expanded. The device expands the range of the temperature of the leaf chamber regulated and controlled by the LI-6400XT portable photosynthetic measurement system from the original environment temperature of +/-7 ℃ to 5-40 ℃, but the measured leaves are limited.
Chinese patent application 201720515458.X, the title of the invention, "photosynthetic assay air inlet auxiliary system for uniform dust fall" discloses a photosynthetic assay air inlet auxiliary system for uniform dust fall, which comprises an air inlet filtration homogenizing device and an air inlet connecting pipe, wherein the air inlet filtration homogenizing device comprises a hanging rope, a filter membrane fixing cover, an air inlet buffering barrel and an air inlet pipe connecting cover, a first external thread is arranged on the top side of the outer side wall of the air inlet buffering barrel, a second external thread is arranged on the bottom side of the outer side wall of the air inlet buffering barrel, a first internal thread is arranged on the inner side wall of the filter membrane fixing cover, the filter membrane fixing cover is connected with the top of the air inlet buffering barrel, two hanging rope holes are symmetrically arranged on the top side of the filter membrane fixing cover, the filter membrane fixing cover is fixedly connected with the hanging rope, the filter membrane is mutually extruded and fixed with the air inlet buffering barrel through the filter membrane fixing, the air inlet pipe connecting cover is fixedly connected with the bottom of the air inlet buffering barrel, and one end of the air inlet connecting pipe penetrates through the air inlet pipe connecting hole to be clamped with the rubber ring. Although the area of the leaf chamber is increased, the space of the leaf chamber is large, air can not circulate, and the photosynthesis measurement is inaccurate; the self-made leaf chamber is fixed through the hanging rope, the blades at different angles cannot be measured, the size of the leaf chamber cannot be adjusted along with the size of the blades, and the self-made leaf chamber is not suitable for high-precision measurement of a single blade.
Chinese patent application 201711446350.0 entitled "device for extending temperature control range of photosynthesis determination system by circulating alcohol" discloses a device for extending temperature control range of photosynthesis determination system by circulating alcohol, which utilizes circulating pump to deliver cooled or heated liquid alcohol, and sends the liquid alcohol to a metal part (containing a plurality of water channels for flowing alcohol inside) tightly attached to LI-6400XT photosynthesis measurement chamber by PVC pipe, and changes the temperature of the metal part; the temperature of the photosynthesis measuring chamber is gradually adjusted through the heat exchange process, and finally, the temperature control range of the portable photosynthesis measuring system (LI-6400XT) is effectively expanded. The device expands the range of regulating and controlling the leaf chamber temperature of the LI-6400XT portable photosynthesis determination system from the original environment temperature of +/-7 ℃ to 5-40 ℃, so that the photosynthesis parameters of the plants/crops in the field determination are not limited by the external environment temperature, and a measurement technology and a measurement method are provided for deeply discussing the response and adaptability mechanism of the physiological and ecological processes of the plants/crops to the temperature under the field condition. Because the invention has the similar defects with the photosynthetic apparatus, the invention can be only used for quantitative detection of partial leaves and is not suitable for high-quality detection of the whole leaves.
Chinese patent application 201920309027.7, the title of the invention, "photosynthesis tester with automatic direction adjustment", discloses a tester, which comprises a base, wherein a photosynthesis tester is arranged on the base, the photosynthesis tester is arranged on the base through a turntable, a transmission gear is arranged at the lower end of the turntable, a rotating motor is arranged beside the base, a speed change gear is arranged on a rotating shaft of the rotating motor, the transmission gear is meshed with the speed change gear, a photosensitive sensor is arranged around the photosynthesis tester, the direction of real-time sunlight is detected through the photosensitive sensor, and the rotating motor is controlled to drive the turntable to rotate. Carry out photosynthesis through the photosynthesis apparatus and detect to drive the carousel level through rotating the motor and rotate, can't survey the blade of different angles.
The Chinese patent application 201910195243.8 entitled fast determination device for photosynthetic rate and transpiration rate of cotton boll leaf system discloses a rapid determination device comprising a plant photosynthetic determinator host, an IRGA sample chamber of an IRGA analyzer, and a cluster-shaped leaf chamber mounting plate, wherein the IRGA sample chamber of the IRGA analyzer is connected with the plant photosynthetic determinator host through a cable, and the rapid determination device is characterized in that a cover plate of the IRGA sample chamber of the IRGA analyzer is removed and connected with the cluster-shaped leaf chamber mounting plate, a self-made leaf chamber is mounted on the cluster-shaped leaf chamber mounting plate, and a vent hole is arranged on the connecting plate, so that the self-made leaf chamber, the cluster-shaped leaf chamber mounting plate and the IRGA sample chamber are communicated through the vent hole. Although the self-made blade chamber is circulated by the fan, the air in the blade chamber cannot be uniform, blades at different angles cannot be measured, the size of the blade chamber cannot be adjusted along with the size of the blades, and the self-made blade chamber is not suitable for high-precision measurement of a single blade.
Therefore, the measurement method using a classical Licor-6800 light combination measuring instrument or a similar improved device or the existing real-time online large-area photosynthetic detection method cannot meet the aim of accurately measuring the photosynthetic intensity generated by a single leaf in different growth directions (angles), and cannot ensure the stability of the carbon dioxide concentration in the long-time measurement process.
Disclosure of Invention
Therefore, the problem to be solved by the present invention is: at any time, carry out the photosynthesis survey to the plant leaf of different growth angles, can realize better under the condition that does not influence the illumination of natural light and completely cut off with the outside air and carry out the accuracy survey, maintain the stability of carbon dioxide concentration, realize that its photosynthesis intensity is surveyed for a long time under the condition of not damaging the leaf in whole testing process to avoid the defect that prior art exists.
Therefore, the invention aims to overcome the defects of the existing equipment and provide the portable field analysis system for measuring the photosynthesis of the plant in real time, which has the advantages of different growth angles of the leaves, high accuracy and no damage to the leaves.
In order to achieve the technical purpose, the invention makes the air in the external blade chamber circulate through a circulating pump on the basis of the traditional blade chamber or the external blade chamber, adopts a sealing ring to be isolated from the outside air, uses light-transmitting materials such as organic glass and the like to solve the problem of light source by directly utilizing the irradiation of natural light, and fixes the external blade chamber for blades with different growth angles through a multi-angle fixing frame.
Therefore, one of the principles of the invention is that on the basis of measuring photosynthesis in the traditional leaf chamber, the air in the leaf chamber is circulated, so that the oxygen and carbon dioxide concentrations at each part of the measured leaf are consistent, and the phenomenon that the measurement value is generally reduced (causing system errors) due to the fact that the photosynthesis speed is directly influenced by the difference of the carbon dioxide concentrations is avoided.
The second principle of the invention is that, in the technology of the traditional external circulation leaf chamber, the full-transparent material such as organic glass is used, the light source problem of photosynthesis can be solved by directly utilizing the irradiation of natural light, and the interference under the external condition is avoided by combining the isolation of a sealing ring and the external air, so that the photosynthetic intensity of each leaf under the natural illumination condition is measured.
The third principle of the invention is that in order to ensure that the blades with different growth angles are measured, the multi-angle fixing device realizes multi-direction fixing of the external leaf chamber, the growth state of the blades is not changed when the photosynthetic intensity is measured, the good growth state of the measured blades and plants is more favorably kept, and the blockage, damage or damage of veins of the blades caused by the measurement of the photosynthetic intensity is avoided.
Accordingly, it is an object of the present invention to provide an angle-adjustable analysis system for real-time measurement of photosynthesis in plants in the field, the system comprising: an external circulating leaf chamber 3 is formed by organic glass 1 and a sealing ring 2, the external circulating leaf chamber 3 is communicated with a carbon dioxide absorption tank 11 through an organic hose 16 by a three-way valve 15, the carbon dioxide absorption tank 11 is communicated with a circulating pump 4 through an organic hose by a three-way valve 17, the external circulating leaf chamber 3 is communicated with the circulating pump 4 through an organic hose 5, the external circulating leaf chamber 3 is fixed on a multi-angle fixing frame 6 through a metal support rod 7, a carbon dioxide concentration detection probe 8 is arranged in the external circulating leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with a carbon dioxide detector 10 through an optical fiber 9, the carbon dioxide absorption tank 11 is communicated with the circulating pump 4 and the external circulating leaf chamber 3 through the organic hose 16, a carbon dioxide supply pipe 13 is communicated with the organic hose 5, and a three-way electronic control valve 14 is arranged, the organic hose 5 is controlled to be communicated with the circulating pump 4 through a three-way valve 15, and the carbon dioxide supply pipe 13 is communicated with a carbon dioxide storage tank (or generating tank) 18 through the organic hose 5.
When the photosynthesis of the plant is measured in real time in the field, the system can adopt a plurality of valves to isolate a carbon dioxide absorption pool 11 from a carbon dioxide storage tank 18, and does not carry out additional treatment on the gas in the operation of a pipeline, and can detect the content change of the carbon dioxide in an external circulating leaf chamber 3 in real time, the external circulating leaf chamber 3 passes through an organic hose 12 through a three-way valve 15 and a three-way valve 17 and is communicated with a circulating pump 4 through an organic hose 5 through a three-way electronic control valve 14, the external circulating leaf chamber 3 is fixed on a multi-angle fixing frame 6 through a metal supporting rod 7, a carbon dioxide concentration detection probe 8 is arranged in the external circulating leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with a carbon dioxide detector 10 through an optical fiber 9.
In any of the above embodiments, the organic glass 1 has a light transmittance of 80% or more, a thickness of 1-10mm, and a cylindrical, rectangular, spherical or the like shape.
In any of the above embodiments, the multi-angle fixing frame 6 is fixed at any angle.
In any of the above embodiments, the depth of the carbon dioxide concentration detection probe 8 placed in the external circulation leaf chamber 3 is 5-50 mm, preferably 15 mm.
In any of the above embodiments, the carbon dioxide absorption cell 11 is composed of 0.1-0.5 mol/L sodium hydroxide aqueous solution, and the amount of carbon dioxide absorbed is converted by measuring the change of pH in the solution or the amount of carbon dioxide generated by the neutralization reaction of sulfuric acid is directly measured, and the temperature of the solution can be controlled by a temperature reduction device, so as to directly influence the temperature change in the external circulation leaf chamber 3.
In any of the above embodiments, the carbon dioxide storage tank or generation tank 18, from a carbon dioxide storage tank with a pressure higher than 1 atm or from the reaction of sodium carbonate with dilute sulfuric acid, determines the discharge rate of carbon dioxide through the electronically controlled valve 14, and maintains the carbon dioxide concentration in the organic hose 5 at 0.03% in relation to the air circulation rate of the circulation pump 4, and then adjusts the carbon dioxide concentration appropriately according to the detection data of the carbon dioxide detector 10.
Drawings
FIG. 1 is a schematic diagram of an analytical system for real-time determination of plant photosynthesis in the field; wherein 1: organic glass; 2: a seal ring; 3: an external circulating leaf chamber; 4: a circulation pump; 5: an organic hose; 6: a multi-angle fixing frame; 7: a metal strut; 8: a carbon dioxide concentration detection probe; 9: an optical fiber; 10: a carbon dioxide detector; 11: a carbon dioxide absorption tank; 12: an organic hose; 13: a carbon dioxide supply pipe; 14: a three-way electronic control valve; 15: a three-way valve; 16: an organic hose; 17: a three-way valve; 18: a carbon dioxide storage tank or a production tank.
Technical effects
1. The invention can make the carbon dioxide concentration in the leaf chamber completely the same through the external circulating pump, and avoids the uneven carbon dioxide concentration caused by photosynthesis in the leaf chamber.
2. When the multi-angle fixing frame is used for measuring the photosynthesis, the measurement of the photosynthetic intensity of the leaves at different growth angles of the plant cannot be influenced, the photosynthetic intensity of the leaves at different growth angles can be more conveniently compared in real time, the working intensity of operators is greatly reduced, and a very efficient tool is provided for basic research of plant photosynthesis and biological engineering research.
3. The method provided by the invention firstly absorbs carbon dioxide in the air and replenishes carbon dioxide at a later stage, so that feasibility is provided for a long-time experiment for measuring the photosynthetic strength of the whole plant or a single leaf, and meanwhile, the temperature rise of the closed environment of a leaf chamber can be effectively slowed down, and the stability of the environment humidity is effectively maintained.
Detailed Description
The invention is further illustrated with reference to the following figures and examples. However, the following description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention will still fall within the scope of the technical solution of the present invention without departing from the technical solution of the present invention.
As shown in FIG. 1, a schematic diagram of an analysis system for real-time determination of plant photosynthesis in the field is shown. Wherein, organic glass 1 and a sealing ring 2 are combined to form an external circulating leaf chamber 3, the external circulating leaf chamber 3 is communicated with a carbon dioxide absorption tank 11 through an organic hose 16 by a three-way valve 15, the carbon dioxide absorption tank 11 is communicated with a circulating pump 4 through an organic hose by a three-way valve 17, the external circulating leaf chamber 3 is communicated with the circulating pump 4 through an organic hose 5, the external circulating leaf chamber 3 is fixed on a multi-angle fixing frame 6 through a metal support rod 7, a carbon dioxide concentration detection probe 8 is arranged in the external circulating leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with a carbon dioxide detector 10 through an optical fiber 9, the carbon dioxide absorption tank 11 is communicated with the circulating pump 4 and the external circulating leaf chamber 3 through the organic hose 16, a carbon dioxide supply pipe 13 is communicated with the organic hose 5, a three-way electronic control valve 14 is arranged in the middle, the organic hose 5 is controlled to be communicated with the circulating pump 4 through a three-way valve 15, and the carbon dioxide supply pipe 13 is communicated with a carbon dioxide storage tank (or generating tank) 18 through the organic hose 5.
The first embodiment is as follows: method for detecting photosynthesis of rice leaves in real time by using any one of analysis systems
Firstly, opening a sealing ring 2, selecting rice leaves or a whole plant of rice to be placed in organic glass 1, combining the organic glass 1 and the sealing ring 2 to form an external circulating leaf chamber 3, communicating the external circulating leaf chamber 3 with a carbon dioxide absorption pool 11 through an organic hose 16 by a three-way valve 15, communicating the carbon dioxide absorption pool 11 with a circulating pump 4 through an organic hose by a three-way valve 17, communicating the external circulating leaf chamber 3 with the circulating pump 4 through an organic hose 5, fixing the external circulating leaf chamber 3 on a multi-angle fixing frame 6 through a metal supporting rod 7, placing a carbon dioxide concentration detection probe 8 in the external circulating leaf chamber 3, communicating the carbon dioxide concentration detection probe 8 with a carbon dioxide detector 10 through an optical fiber 9, opening the carbon dioxide detector 10, communicating the carbon dioxide absorption pool 11 with the circulating pump 4 and the circulating leaf chamber 3 through the organic hose 16, communicating a carbon dioxide supply pipe 13 with the, the middle part is provided with a three-way electronic control valve 14 for controlling the flow rate of carbon dioxide or closing the supply of carbon dioxide, the three-way valve 15 is used for controlling the organic hose 5 to be communicated with the circulating pump 4, the carbon dioxide supply pipe 13 is communicated with a carbon dioxide storage tank (or generation tank) 18 through the organic hose 5, and the carbon dioxide concentration in the leaf chamber is controlled to be kept at 0.03% by detecting data through a carbon dioxide detector 10. The consumption amount of carbon dioxide in the whole photosynthetic process is calculated by measuring the pH change (amount of absorbed carbon dioxide) of the carbon dioxide absorption cell 11 and the input amount of the carbon dioxide supply tube 13 (amount of supplied carbon dioxide).
Example two: method for detecting photosynthesis of rice leaves in real time by using short-time analysis system
Firstly, a sealing ring 2 is opened, rice leaves or the whole rice is selected to be placed in organic glass 1, the organic glass 1 and the sealing ring 2 are combined to form an external circulating leaf chamber 3, the external circulating leaf chamber 3 is communicated with a circulating pump 4 through an organic hose 12 through a three-way valve 15 and a three-way valve 17 and an organic hose 5 through a three-way electronic control valve 14, the external circulating leaf chamber 3 is fixed on a multi-angle fixing frame 6 through a metal supporting rod 7, a carbon dioxide concentration detection probe 8 is placed in the external circulating leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with a carbon dioxide detector 10 through an optical fiber 9, the carbon dioxide detector 10 is opened, and the three-way electronic control valve 14 is. After 1 hour, the consumption of carbon dioxide in the whole photosynthetic process is directly read by a carbon dioxide detector 10.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (7)
1. An angle-adjustable field real-time plant photosynthesis assay system, comprising: an external circulating leaf chamber 3 is formed by organic glass 1 and a sealing ring 2, the external circulating leaf chamber 3 is communicated with a carbon dioxide absorption tank 11 through an organic hose 16 by a three-way valve 15, the carbon dioxide absorption tank 11 is communicated with a circulating pump 4 through an organic hose by a three-way valve 17, the external circulating leaf chamber 3 is communicated with the circulating pump 4 through an organic hose 5, the external circulating leaf chamber 3 is fixed on a multi-angle fixing frame 6 through a metal support rod 7, a carbon dioxide concentration detection probe 8 is arranged in the external circulating leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with a carbon dioxide detector 10 through an optical fiber 9, the carbon dioxide absorption tank 11 is communicated with the circulating pump 4 and the external circulating leaf chamber 3 through the organic hose 16, a carbon dioxide supply pipe 13 is communicated with the organic hose 5, and a three-way electronic control valve 14 is arranged, the organic hose 5 is controlled to be communicated with the circulating pump 4 through a three-way valve 15, and the carbon dioxide supply pipe 13 is communicated with a carbon dioxide storage tank 18 through the organic hose 5.
2. The analytical system according to claim 1, wherein when the real-time short-time measurement is performed in the field, the external circulating leaf chamber 3 is connected to the circulating pump 4 through the organic hose 12 via the three-way valve 15 and the three-way valve 17 and through the organic hose 5 via the three-way electronic control valve 14, the external circulating leaf chamber 3 is fixed to the multi-angle fixing frame 6 via the metal support rod 7, the carbon dioxide concentration detecting probe 8 is placed in the external circulating leaf chamber 3, the carbon dioxide concentration detecting probe 8 is connected to the carbon dioxide detecting instrument 10 via the optical fiber 9, and the three-way electronic control valve 14 is closed.
3. The analytical system according to claims 1-2, wherein the organic glass 1 has a light transmittance of 80% or more, a thickness of 1-10mm, and a cylindrical, rectangular, spherical shape or the like.
4. The analysis system according to claims 1-2, wherein said multi-angle holder 6 is a holder of any angle.
5. The analysis system according to claims 1-2, wherein the carbon dioxide concentration detecting probe 8 is placed in the external circulation leaf chamber 3 to a depth of 5-50 mm, preferably 15 mm.
6. The analysis system according to claim 1-2, wherein the carbon dioxide absorption cell 11 is composed of 0.1-0.5 mol/L sodium hydroxide aqueous solution, the absorption amount of carbon dioxide is calculated by measuring the change of pH value in the solution or the amount of carbon dioxide generated by the neutralization reaction of sulfuric acid is directly measured, and the temperature of the solution can be controlled by a temperature reduction device to directly influence the temperature change in the external circulation leaf chamber 3.
7. The analysis system according to claims 1-2, wherein said carbon dioxide storage or generation tank 18, from which carbon dioxide is produced by the reaction of sodium carbonate with dilute sulphuric acid or from a carbon dioxide storage tank at a pressure higher than 1 atmosphere, is controlled by an electronically controlled valve 14 to determine the discharge rate of carbon dioxide, which is related to the circulation rate of air by the circulation pump 4, by maintaining the carbon dioxide concentration in the organic hose 5 at 0.03%, and by adjusting the detected data appropriately by the carbon dioxide detector 10.
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| CN202110450311.8A CN113063902A (en) | 2021-04-25 | 2021-04-25 | Portable analysis system for field real-time determination of plant photosynthesis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114002194A (en) * | 2021-10-22 | 2022-02-01 | 安徽袁粮水稻产业有限公司 | Method for identifying photosynthetic capacity of improved rice plant |
| CN114354549A (en) * | 2021-12-09 | 2022-04-15 | 江苏省中国科学院植物研究所 | Portable device for measuring underwater photosynthesis of plants in field and method for synchronously measuring photosynthetic rate and dark respiration rate by using portable device |
| CN114814104A (en) * | 2022-04-28 | 2022-07-29 | 湖南杂交水稻研究中心 | Device for inducing photosynthesis of single-sided leaves |
| CN117420275A (en) * | 2023-12-18 | 2024-01-19 | 西安多普多信息科技有限公司 | Photosynthetic rate detection apparatus, photosynthetic rate detection method, storage medium, and electronic device |
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2021
- 2021-04-25 CN CN202110450311.8A patent/CN113063902A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114002194A (en) * | 2021-10-22 | 2022-02-01 | 安徽袁粮水稻产业有限公司 | Method for identifying photosynthetic capacity of improved rice plant |
| CN114354549A (en) * | 2021-12-09 | 2022-04-15 | 江苏省中国科学院植物研究所 | Portable device for measuring underwater photosynthesis of plants in field and method for synchronously measuring photosynthetic rate and dark respiration rate by using portable device |
| CN114354549B (en) * | 2021-12-09 | 2024-03-08 | 江苏省中国科学院植物研究所 | Portable plant underwater photosynthesis measuring device for field and method for synchronously measuring photosynthesis rate and dark respiration rate |
| CN114814104A (en) * | 2022-04-28 | 2022-07-29 | 湖南杂交水稻研究中心 | Device for inducing photosynthesis of single-sided leaves |
| WO2023208030A1 (en) * | 2022-04-28 | 2023-11-02 | 湖南杂交水稻研究中心 | Apparatus for inducing single-side leaf photosynthesis |
| CN117420275A (en) * | 2023-12-18 | 2024-01-19 | 西安多普多信息科技有限公司 | Photosynthetic rate detection apparatus, photosynthetic rate detection method, storage medium, and electronic device |
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