CN114002194A - Method for identifying photosynthetic capacity of improved rice plant - Google Patents
Method for identifying photosynthetic capacity of improved rice plant Download PDFInfo
- Publication number
- CN114002194A CN114002194A CN202111232782.8A CN202111232782A CN114002194A CN 114002194 A CN114002194 A CN 114002194A CN 202111232782 A CN202111232782 A CN 202111232782A CN 114002194 A CN114002194 A CN 114002194A
- Authority
- CN
- China
- Prior art keywords
- sword
- leaf
- sliding
- photosynthetic
- block
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N2021/635—Photosynthetic material analysis, e.g. chrorophyll
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an identification method of photosynthetic capacity of an improved rice plant, which relates to the technical field of rice planting.A portable photosynthetic rate tester is arranged on a continuous tester, the portable photosynthetic rate tester is controlled by the continuous tester to intermittently move forwards, and the photosynthetic rates of a plurality of rice plants are continuously measured in the process of moving forwards; the measured photosynthetic rate parameters are led into a computer for processing and analysis, and then the photosynthetic capacity of the rice plants can be obtained. The invention uses the continuous determination device to control the portable photosynthetic rate determinator to intermittently and continuously determine the sword leaf photosynthesis parameters of a plurality of samples, thereby effectively shortening the single measurement time of the plurality of samples of the portable photosynthetic rate determinator and shortening the time span of the single measurement.
Description
Technical Field
The invention relates to the technical field of rice planting, in particular to an identification method of improved rice plant photosynthetic capacity.
Background
The conventional rice plant photosynthetic parameter is mostly determined by adopting a portable photosynthetic rate determinator, the sword leaves of rice are manually clamped and determined one by utilizing a leaf chamber at the front end of a handheld part of the portable photosynthetic rate determinator, and the sword leaves are determined on living plants and cannot be damaged because the sword leaves are only one and need to be determined for many times.
In the experimental state, because the plant number is less, can carry out parameter determination piece by piece through the manual work, but in the field planting state, when many samples of a plurality of materials parameter determination simultaneously, because sample quantity and planting area are all great, not only waste time and energy during manual measurement, more importantly, the time span of measuring all samples once increases, the time point difference that the parameter of determining from beginning to end corresponds is great, it is inaccurate to cause the measured value of parameter, the parameter of same batch survey can't carry out the parallel contrast, the time difference of measuring can't stagger around the different samples of same material, can't satisfy the demand of once of every two hours or shorter time survey.
Disclosure of Invention
The invention aims to provide an identification method of photosynthetic capacity of an improved rice plant, and aims to solve the problems of low speed, discontinuity and large single measurement time span when a portable photosynthetic rate tester measures parameters under the condition of multi-sample field planting.
A method for identifying photosynthetic capacity of an improved rice plant comprises the following specific steps:
(1) selecting rice plants with completely extracted and unfolded sword leaves to measure photosynthetic related indexes;
(2) determination of daily change of photosynthetic rate: selecting a clear high-temperature weather, measuring the photosynthetic rate and the stomatal conductance of the sword leaves of different rice materials every 2 hours from 9 to 17 points, repeatedly measuring at least 10 rice materials for each rice material, selecting the same part of each rice sword leaf to carry out multiple measurements during measurement, wherein the measured part is the middle part of the sword leaves, and the photosynthetic rate measurement is carried out by adopting a portable photosynthetic rate measuring instrument and an external carbon dioxide system;
(3) the portable photosynthetic rate measuring instrument is arranged on the continuous measuring device, the continuous measuring device controls the portable photosynthetic rate measuring instrument to intermittently move forwards, and the photosynthetic rates of a plurality of rice plants are continuously measured in the process of moving forwards;
(4) the measured photosynthetic rate parameters are led into a computer for processing and analysis, and then the photosynthetic capacity of the rice plants can be obtained.
Preferably, the continuous measurement method in step (3) is:
(a) firstly, arranging a continuous measuring device at one side of a rice plant, sticking magnetic pastes at the upper end and the lower end of the back of a sword leaf of a single rice plant, adjusting the distance between a sword leaf delivery mechanism and the sword leaf until the magnetic pastes are adsorbed at the upper part of the sword leaf delivery mechanism, and flattening and supporting the sword leaf by a sword leaf delivery mechanism; according to the method, the flag leaves of a plurality of rice plants are adsorbed and fixed on one side of a continuous measuring device;
(b) the portable photosynthetic rate determinator is driven forwards along a straight line to move intermittently as a whole, the sword leaf submission mechanism is stopped and triggered when the portable photosynthetic rate determinator moves to the sword leaf submission mechanism, the sword leaf submission mechanism presses down the sword leaves and transversely places the sword leaves in front of a leaf chamber of the portable photosynthetic rate determinator, then an electric cylinder pushes the leaf chamber to the sword leaves, when the sword leaves enter the range of the leaf chamber, the leaf chamber clamps the sword leaves in a closing manner in the pushing process to carry out measurement, and the sword leaf submission mechanism maintains the transverse state in the measuring process;
(c) retracting the leaf chamber after the measurement is finished, and opening the leaf chamber to separate from the blade once the leaf chamber is retracted until the leaf chamber is reset;
(d) the portable photosynthetic rate determinator moves forwards continuously, the sword leaf inspection mechanism lifts upwards to reset in the continuous moving process, and the photosynthetic rates of a plurality of rice plants can be determined continuously by repeating the operation when the sword leaf inspection mechanism is triggered again by the integral forward movement of the portable photosynthetic rate determinator.
Preferably, the continuous measuring device comprises a main chute arranged on one side of a rice plant, a plurality of support legs are arranged below the main chute, a main through groove is arranged at the bottom of the main chute, a main screw rod is rotatably connected between two ends of the main chute, a motor is arranged at one end of the main chute, one end of the main screw rod is connected with an output shaft of the motor, a slide seat is connected in the main chute in a sliding manner, the slide seat is in threaded connection with the main screw rod, an electric cylinder and a portable photosynthetic measuring instrument are arranged on the slide seat, a handheld part of the portable photosynthetic measuring instrument stretches out and draws back along with opening and closing actions under the control of the electric cylinder, a trapezoidal block is arranged at the front end of the bottom of the slide seat, the top of the trapezoidal block is connected in the main through groove in a sliding manner, a plurality of sets of sword leaf feeding and detecting mechanisms are hung outside the main chute, and when a front inclined plane of the trapezoidal block passes through the bottom of the sword leaf feeding and detecting mechanisms, the sword leaf feeding and detecting mechanisms gradually press down the sword leaves horizontally in front of the handheld part of the portable photosynthetic measuring instrument, when the middle plane of the trapezoid block passes through the bottom of the inspection mechanism, the sword leaf inspection mechanism maintains the transverse state of the sword leaves, and when the rear inclined plane of the trapezoid block passes through the bottom of the inspection mechanism, the sword leaf inspection mechanism gradually lifts the sword leaves to complete reset.
Preferably, portable photosynthetic apparatus's handheld part includes the grab handle, presses handle and leaf room, press to be equipped with spring one between handle and the grab handle, press the front end of handle to connect leaf room upper portion, the rear end of pressing the handle transversely is equipped with the depression bar, the leaf room lower part is connected to the front end of grab handle, and the rear end of grab handle is connected with the telescopic link top of electric jar, be equipped with the frame on the slide, the front end of frame is the wedge, the both ends and the frame bottom surface roll butt of depression bar, be equipped with portable photosynthetic apparatus's host computer on the frame, the leaf room passes through the hose and the electric wire is connected with the host computer, the front portion of slide still is equipped with the T-shaped groove, sliding connection has T shape slider in the T-shaped groove, the top and the grab handle of T shape slider link firmly.
Preferably, the sword leaf inspection mechanism comprises a first concave block and a second concave block which are symmetrically arranged at two sides of a main sliding groove, a first through groove and a second through groove are respectively formed at the same side of the first concave block and the second concave block, the first through groove and the second through groove are coaxial, a base plate is connected in the first concave block in a sliding manner, a sliding block is arranged at one end of the base plate, the sliding block is connected with the first through groove in a sliding manner, a first lifting sliding groove is formed in the sliding block, a lifting sliding rod is connected in the first lifting sliding groove in a sliding manner, a mounting block is connected in the second concave block in a sliding manner, a second sliding block is arranged at one side of the mounting block, the second sliding block is connected with the second through groove in a sliding manner, a second lifting sliding groove is formed in the second sliding block, the first sliding block and the second sliding block are respectively penetrated through the lower parts of the two lifting sliding rods, and the two lifting sliding rods are respectively connected with the first sliding block and the second sliding block in a sliding manner, be equipped with the spreader between two lift slide bar's the lower extreme, the below of main spout is located to the spreader, be equipped with spring two between the one end of spreader and the slider one, also be equipped with spring two between the other end of spreader and the slider two, the top of installation piece is rotated and is connected with the pivot, the one end of pivot is equipped with the gear, the gear meshes with the lift slide bar upper portion of homonymy, radially be equipped with concave frame in the pivot, the symmetry is equipped with two adsorption tanks on the concave frame, transverse rotation is connected with screw rod two in the concave block two, screw rod two and installation piece threaded connection.
The invention has the advantages that:
the invention uses the continuous determination device to control the portable photosynthetic rate determinator to intermittently and continuously determine the sword leaf photosynthesis parameters of a plurality of samples, thereby effectively shortening the single measurement time of the plurality of samples of the portable photosynthetic rate determinator and shortening the time span of the single measurement. The continuous measurement mode can greatly reduce the time consumption of single measurement, can meet the requirement of single measurement when a large number of samples are obtained, and does not delay time points. The time span for measuring the parameters in the same batch is smaller, the error between the data at the same time point is smaller, and the identification result is more accurate.
Drawings
FIGS. 1 and 2 are schematic views showing the overall structure of a continuous measuring apparatus from different viewing angles.
FIG. 3 is a plan view of the continuous measuring apparatus.
Fig. 4 is a sectional view taken along line a-a in fig. 3.
Fig. 5 is a sectional view taken along line C-C in fig. 3.
FIG. 6 is a side view of the continuous measuring apparatus.
Wherein, 1-a main sliding groove, 11-a motor, 12-a main screw rod, 13-a support leg, 14-a main through groove, 2-a sliding seat, 21-a framework, 22-an electric cylinder, 23-a T-shaped groove, 3-a main machine, 31-a leaf chamber, 32-a pressing handle, 33-a holding handle, 34-a pressing rod, 35-a first spring, 4-a first concave block, 41-a base plate, 42-a first lifting sliding groove, 43-a first through groove, 44-a first sliding block, 45-a second sliding block, 5-a transverse roller, 51-a lifting sliding rod, 52-a second spring, 6-a second concave block, 61-an installation block, 62-a second lifting sliding groove, 63-a gear, 64-a rotating shaft, 65-a concave frame, 66-a second screw rod, 67-an adsorption groove and 68-a second through groove, 7-trapezoidal block, 8-T slider.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1: a method for identifying photosynthetic capacity of an improved rice plant comprises the following specific steps:
step one, selecting rice plants with flag leaves completely extracted and unfolded to carry out determination of photosynthetic related indexes, wherein the whole determination process is carried out under field planting conditions;
step two, measuring the daily change of the photosynthetic rate: selecting a clear high-temperature weather, measuring the photosynthetic rate and the stomatal conductance of the sword leaves of different rice materials every 2 hours from 9 to 17 points, repeatedly measuring at least 10 rice materials for each rice material, selecting the same part of each rice sword leaf to carry out multiple measurements during measurement, wherein the measured part is the middle part of the sword leaves, and the photosynthetic rate measurement is carried out by adopting a portable photosynthetic rate measuring instrument and an external carbon dioxide system; in this example, photosynthetic capacity of 10 rice materials was measured, and 10 rice individuals were measured for each rice material.
Step three, arranging the portable photosynthetic rate measuring instrument on a continuous measuring device shown in figures 1 to 6, controlling the portable photosynthetic rate measuring instrument to intermittently move forwards through the continuous measuring device, and continuously measuring the photosynthetic rates of a plurality of rice plants in the moving-forwards process, wherein the specific method comprises the following steps:
firstly, the main chute 1 and the support legs 13 are arranged on one side of a rice plant, light and thin magnetic pastes are pasted at the upper end and the lower end of the back of a single-plant rice sword leaf, the screw rod II 66 is rotated, the position of the mounting block 61 is adjusted, the mounting block 61 drives the slide block II 45 to slide in the through groove II 68, the slide block I44 drives the lifting slide rod 51 and the transverse roller 5 to transversely move, the transverse roller 5 drives the substrate 41 and the slide block I44 to horizontally slide, so that the distance between the concave frame 65 and the sword leaf is adjusted until the magnetic pastes are adsorbed in the two adsorption grooves 67 on the concave frame 65, the concave frame 65 is used for flattening and supporting the sword leaf, the sword leaf can be more softly fixed on the concave frame 65 by adopting a magnetic adsorption mode, and the sword leaf is prevented from being broken or damaged; the flag leaves of a plurality of rice plants are adsorbed and fixed on the corresponding concave frames 65 according to the method;
during measurement, the motor 11 is started, the motor 11 drives the main screw 12 to rotate, the main screw 12 drives the sliding seat 2 and the trapezoidal block 7 to move forward, when the front end inclined plane of the trapezoidal block 7 contacts the cross roller 5, the cross roller 5 abuts against the front inclined plane of the trapezoidal block 7 under the action of the springs 52 on the two sides, along with the forward movement of the trapezoidal block 7, the cross roller 5 rolls on the front inclined plane of the trapezoidal block 7 to move downwards, the cross roller 5 moves downwards to drive the lifting slide rods 51 on the two sides to slide in the lifting slide grooves 42 and the lifting slide grooves 62 respectively to move downwards, the gear 63 is pulled to rotate when the lifting slide rods 51 move downwards, the gear 63 drives the rotating shaft 64 to rotate, the rotating shaft 64 drives the concave frame 65 and the sword leaf to rotate around the rotating shaft 64, when the cross roller 5 rolls to the bottom plane of the trapezoidal block 7, the concave frame 65 and the sword leaf are just transversely arranged in front of the leaf chamber 31, at the moment, the motor 11 stops rotating, the sliding seat 2 stops moving forward, the cross roller 5 stops at the bottom plane of the trapezoidal block 7, the concave frame 65 is kept in a horizontal state, the middle part of the sword leaf is horizontally arranged in front of the leaf chamber 31 to wait for detection, at the moment, the electric cylinder 22 is opened, the electric cylinder 22 pushes the leaf chamber 31, the pressing handle 32 and the pressing rod 34 to move forwards through the grab handle 33, two ends of the pressing rod 34 roll along the lower edge of the frame 21 under the action of the first spring 35, when the pressing rod 34 rolls to the wedge-shaped inclined surface at the front end of the frame 21, the pressing rod 34 moves upwards, the first spring 35 pushes the pressing handle 32 upwards, the upper part and the lower part of the leaf chamber 31 are gradually clamped, and the sword leaf horizontally arranged in front is clamped along with clamping action along with the forward movement of the leaf chamber 31 to be detected. After detection is finished, the electric cylinder 22 is opened to retract the vane chamber 31, and the vane chamber 31 is immediately opened under the action of the wedge-shaped inclined surface to prevent the vanes from being damaged by pulling. After the retraction leaf chamber 31 is reset, the motor 11 is started, the sliding seat 2 is continuously moved forwards, at the moment, the transverse roller 5 rolls to the rear inclined plane of the trapezoidal block 7, the transverse roller 5 and the lifting slide rod 51 gradually move upwards, the lifting slide rod 51 pushes the gear 63 to rotate reversely, the concave frame 65 carries the sword leaves to gradually rotate and lift upwards, when the transverse roller 5 is separated from the trapezoidal block 7 to reset, the concave frame 65 resets to the outer side of the main sliding groove 1, the continuous forward movement of the sliding seat 2 cannot be influenced, the sliding seat 2 continuously moves forwards until the next transverse roller 5 is positioned on the bottom plane of the trapezoidal block 7 and stops, and the detection actions are repeated. When the sliding seat 2 moves to the other end of the main sliding chute 1, the motor 11 rotates reversely, the sliding seat 2 moves reversely to the initial end to reset, and the next time point is waited for measuring action.
And step four, the measured photosynthetic rate parameters are guided into a computer for processing and analysis, and then the photosynthetic capacity of the rice plant can be obtained.
The continuous measuring device used in the embodiment comprises a main chute 1 arranged on one side of a rice plant, a plurality of support legs 13 are arranged below the main chute 1, a main through groove 14 is arranged at the bottom of the main chute 1, a main screw 12 is rotatably connected between two ends of the main chute 1, a motor 11 is arranged at one end of the main chute 1, one end of the main screw 12 is connected with an output shaft of the motor 11, a slide seat 2 is connected in the main chute 1 in a sliding manner, the slide seat 2 is in threaded connection with the main screw 12, an electric cylinder 22 and a portable photosynthetic measuring instrument are arranged on the slide seat 2, a handheld part of the portable photosynthetic measuring instrument stretches and retracts under the control of the electric cylinder 22 and moves along with opening and closing, a trapezoidal block 7 is arranged at the front end of the bottom of the slide seat 2, the top of the trapezoidal block 7 is connected in the main through groove 14 in a sliding manner, and a plurality of sword leaf conveying and detecting mechanisms are externally hung on the main chute 1, when the front inclined surface of the trapezoidal block 7 passes through the bottom of the sword leaf delivery mechanism, the sword leaf delivery mechanism gradually presses down the sword leaves and transversely arranges the sword leaves in front of the handheld part of the portable photosynthetic determinator, when the middle plane of the trapezoidal block 7 passes through the bottom of the delivery mechanism, the sword leaf delivery mechanism maintains the state of the sword leaves in the transverse state, and when the rear inclined surface of the trapezoidal block 7 passes through the bottom of the delivery mechanism, the sword leaf delivery mechanism gradually lifts the sword leaves to complete resetting.
The handheld part of above-mentioned portable photosynthetic apparatus includes grab handle 33, pressure handle 32 and leaf room 31, it is equipped with spring 35 to press between handle 32 and the grab handle 33, press the front end of handle 32 to connect leaf room 31 upper portion, the rear end of pressing handle 32 transversely is equipped with depression bar 34, leaf room 31 lower part is connected to the front end of grab handle 33, and the rear end of grab handle 33 is connected with the telescopic link top of electric jar 22, be equipped with frame 21 on the slide 2, the front end of frame 21 is the wedge, the both ends and the rolling butt of frame 21 bottom surface of depression bar 34, be equipped with portable photosynthetic apparatus's host computer 3 on the frame 21, leaf room 31 passes through the hose and is connected with host computer 3 with the electric wire, the front portion of slide 2 still is equipped with T shape groove 23, sliding connection has T shape slider 8 in the T shape groove 23, the top and the 33 of T shape slider 8 link firmly.
The sword leaf inspection mechanism comprises a first concave block 4 and a second concave block 6 which are symmetrically arranged at two sides of a main sliding groove 1, a first through groove 43 and a second through groove 68 are respectively arranged at the same side of the first concave block 4 and the second concave block 6, the first through groove 43 and the second through groove 68 are coaxial, a base plate 41 is connected in the first concave block 4 in a sliding manner, a sliding block is arranged at one end of the base plate 41 and is connected with the first through groove 43 in a sliding manner, a first lifting sliding groove 42 is arranged on the sliding block, a lifting sliding rod 51 is connected in the first lifting sliding groove 42 in a sliding manner, a mounting block 61 is connected in the second concave block 6 in a sliding manner, a second sliding block 45 is arranged at one side of the mounting block 61, the second sliding block 45 is connected with the second through groove 68 in a sliding manner, a second lifting sliding groove 62 is arranged on the second sliding block 45, a lifting sliding rod 51 is also connected in the second lifting sliding manner, and the first sliding block 44 and the second sliding block 45 are respectively penetrated through the lower parts of the two lifting sliding rods 51, two lift slide bars 51 respectively with slider 44 and two 45 sliding connection of slider, be equipped with horizontal roller 5 between two lift slide bars 51's the lower extreme, horizontal roller 5 locates the below of main spout 1, be equipped with two 52 springs between the one end of horizontal roller 5 and the slider 44, also be equipped with two 52 springs between the other end of horizontal roller 5 and the slider 45, the top of installation piece 61 is rotated and is connected with pivot 64, the one end of pivot 64 is equipped with gear 63, gear 63 meshes with the lift slide bar 51 upper portion of homonymy, radially be equipped with concave frame 65 in the pivot 64, the symmetry is equipped with two adsorption tanks 67 on the concave frame 65, transversely rotate in the concave block two 6 and be connected with two 66 screws, two 66 screws and installation piece 61 threaded connection.
Comparative example: the rest of the procedure was the same as in example 1, except that the rice flag leaves were measured one by using a manually operated portable photosynthetic rate measuring instrument.
The time spent in a single measurement of example 1 and the control example was recorded for 4 times, and the comparison results are shown in table 1:
TABLE 1 comparison of the elapsed time for a single measurement of a full sample
| Group of | Time consumed for a single measurement (min) | Measurement of Total number of samples (Strain) |
| Comparative example | 128.7±21.7 | 100 |
| Example 1 | 56.3±2.4 | 100 |
As can be seen from the results in table 1, the manual single measurement takes longer time, the time span is longer, and the measurement duration is also unstable, which is more disadvantageous in that, because the total number of samples is more, the manual single measurement duration exceeds the measurement interval time of 2 hours, which causes the measurement process at the next time point to be delayed or delayed, therefore, more instruments or manpower are required for measurement, which is time-consuming and labor-consuming, the efficiency is low, and because the time spans of the previous and subsequent sample measurements are too large, the data between the samples does not have relative comparability, which results in inaccurate photosynthetic capacity identification result or failure to obtain the identification result. The continuous measurement in the application can greatly reduce the time consumption of single measurement, can meet the requirement of single measurement when a large number of samples are obtained, and does not delay time points. The time span is smaller, the error between the data at the same time point is smaller, and the identification result is more accurate.
It will be appreciated by those skilled in the art that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The embodiments disclosed above are therefore to be considered in all respects as illustrative and not restrictive. All changes which come within the scope of or equivalence to the invention are intended to be embraced therein.
Claims (5)
1. An identification method of improved rice plant photosynthetic capacity is characterized by comprising the following specific steps:
(1) selecting rice plants with completely extracted and unfolded sword leaves to measure photosynthetic related indexes;
(2) determination of daily change of photosynthetic rate: selecting a clear high-temperature weather, measuring the photosynthetic rate and the stomatal conductance of the sword leaves of different rice materials every 2 hours from 9 to 17 points, repeatedly measuring at least 10 rice materials for each rice material, selecting the same part of each rice sword leaf to carry out multiple measurements during measurement, wherein the measured part is the middle part of the sword leaves, and the photosynthetic rate measurement is carried out by adopting a portable photosynthetic rate measuring instrument and an external carbon dioxide system;
(3) the portable photosynthetic rate measuring instrument is arranged on the continuous measuring device, the continuous measuring device controls the portable photosynthetic rate measuring instrument to intermittently move forwards, and the photosynthetic rates of a plurality of rice plants are continuously measured in the process of moving forwards;
(4) the measured photosynthetic rate parameters are led into a computer for processing and analysis, and then the photosynthetic capacity of the rice plants can be obtained.
2. The method for identifying photosynthetic capacity of an improved rice plant as claimed in claim 1, wherein the continuous determination in step (3) is as follows:
(a) firstly, arranging a continuous measuring device at one side of a rice plant, sticking magnetic pastes at the upper end and the lower end of the back of a sword leaf of a single rice plant, adjusting the distance between a sword leaf delivery mechanism and the sword leaf until the magnetic pastes are adsorbed at the upper part of the sword leaf delivery mechanism, and flattening and supporting the sword leaf by a sword leaf delivery mechanism; according to the method, the flag leaves of a plurality of rice plants are adsorbed and fixed on one side of a continuous measuring device;
(b) the portable photosynthetic rate determinator is driven forwards along a straight line to move intermittently as a whole, the sword leaf submission mechanism is stopped and triggered when the portable photosynthetic rate determinator moves to the sword leaf submission mechanism, the sword leaf submission mechanism presses down the sword leaves and transversely places the sword leaves in front of a leaf chamber of the portable photosynthetic rate determinator, then an electric cylinder pushes the leaf chamber to the sword leaves, when the sword leaves enter the range of the leaf chamber, the leaf chamber clamps the sword leaves in a closing manner in the pushing process to carry out measurement, and the sword leaf submission mechanism maintains the transverse state in the measuring process;
(c) retracting the leaf chamber after the measurement is finished, and opening the leaf chamber to separate from the blade once the leaf chamber is retracted until the leaf chamber is reset;
(d) the portable photosynthetic rate determinator moves forwards continuously, the sword leaf inspection mechanism lifts upwards to reset in the continuous moving process, and the photosynthetic rates of a plurality of rice plants can be determined continuously by repeating the operation when the sword leaf inspection mechanism is triggered again by the integral forward movement of the portable photosynthetic rate determinator.
3. The improved method for identifying photosynthetic capacity of rice plants as claimed in claim 1 or 2, wherein the continuous measuring device comprises a main chute (1) arranged at one side of a rice plant, a plurality of support legs (13) are arranged below the main chute (1), a main through groove (14) is arranged at the bottom of the main chute (1), a main screw (12) is rotatably connected between two ends of the main chute (1), a motor (11) is arranged at one end of the main chute (1), one end of the main screw (12) is connected with an output shaft of the motor (11), a sliding seat (2) is slidably connected in the main chute (1), the sliding seat (2) is in threaded connection with the main screw (12), an electric cylinder (22) and a portable photosynthetic measuring instrument are arranged on the sliding seat (2), and a handheld part of the portable photosynthetic measuring instrument is controlled by the electric cylinder (22) to stretch and move along with opening and closing, the sword leaf detection device is characterized in that a trapezoidal block (7) is arranged at the front end of the bottom of the sliding seat (2), the top of the trapezoidal block (7) is connected into the main through groove (14) in a sliding mode, a plurality of groups of sword leaf detection mechanisms are hung on the main sliding groove (1), when the front inclined surface of the trapezoidal block (7) penetrates through the bottom of the sword leaf detection mechanism, the sword leaf detection mechanisms gradually press down the sword leaves and transversely arrange the sword leaves in front of a handheld part of the portable photosynthetic apparatus, when the middle plane of the trapezoidal block (7) penetrates through the bottom of the detection mechanism, the sword leaf detection mechanisms maintain the transverse state of the sword leaves, and when the rear inclined surface of the trapezoidal block (7) penetrates through the bottom of the detection mechanism, the sword leaf detection mechanisms gradually lift the sword leaves to complete reset.
4. The improved method for identifying photosynthetic capacity of rice plants of claim 3, wherein the hand-held part of the portable photosynthetic apparatus comprises a handle (33), a pressing handle (32) and a leaf chamber (31), a first spring (35) is arranged between the pressing handle (32) and the handle (33), the front end of the pressing handle (32) is connected with the upper part of the leaf chamber (31), the rear end of the pressing handle (32) is transversely provided with a pressing rod (34), the front end of the handle (33) is connected with the lower part of the leaf chamber (31), the rear end of the handle (33) is connected with the top of a telescopic rod of the electric cylinder (22), the sliding seat (2) is provided with a frame (21), the front end of the frame (21) is wedge-shaped, two ends of the pressing rod (34) are in rolling contact with the bottom surface of the frame (21), the frame (21) is provided with the host (3) of the portable photosynthetic apparatus, leaf room (31) are connected with host computer (3) through hose and electric wire, the front portion of slide (2) still is equipped with T shape groove (23), sliding connection has T shape slider (8) in T shape groove (23), the top and the grab handle (33) of T shape slider (8) link firmly.
5. The improved rice plant photosynthetic capacity identification method according to claim 4, wherein the sword leaf feeding mechanism comprises a first concave block (4) and a second concave block (6) symmetrically arranged at two sides of the main sliding groove (1), a first through groove (43) and a second through groove (68) are respectively arranged at the same side of the first concave block (4) and the second concave block (6), the first through groove (43) and the second through groove (68) are coaxial, the first concave block (4) is slidably connected with the base plate (41), one end of the base plate (41) is provided with a sliding block, the sliding block is slidably connected with the first through groove (43), the sliding block is provided with a first lifting sliding groove (42), the first lifting sliding groove (42) is slidably connected with a lifting sliding rod (51), the second concave block (6) is slidably connected with a mounting block (61), one side of the mounting block (61) is provided with a second sliding block (45), the second sliding block (45) is connected with the second through groove (68) in a sliding manner, the second sliding block (45) is provided with a second lifting sliding groove (62), a first lifting sliding rod (51) is also connected in the second lifting sliding groove (62) in a sliding manner, the lower parts of the two lifting sliding rods (51) are respectively penetrated by the first sliding block (44) and the second sliding block (45), the two lifting sliding rods (51) are respectively connected with the first sliding block (44) and the second sliding block (45) in a sliding manner, a transverse roller (5) is arranged between the lower ends of the two lifting sliding rods (51), the transverse roller (5) is arranged below the main sliding groove (1), a second spring (52) is arranged between one end of the transverse roller (5) and the first sliding block (44), a second spring (52) is also arranged between the other end of the transverse roller (5) and the second sliding block (45), the top of the mounting block (61) is rotatably connected with a rotating shaft (64), and one end of the rotating shaft (64) is provided with a gear (63), the gear (63) is meshed with the upper part of the lifting slide rod (51) on the same side, a concave frame (65) is arranged on the rotating shaft (64) along the radial direction, two adsorption grooves (67) are symmetrically arranged on the concave frame (65), a second screw rod (66) is connected in the concave block (6) in a transverse rotating mode, and the second screw rod (66) is in threaded connection with the mounting block (61).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111232782.8A CN114002194B (en) | 2021-10-22 | 2021-10-22 | Identification method for photosynthetic capacity of improved rice plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111232782.8A CN114002194B (en) | 2021-10-22 | 2021-10-22 | Identification method for photosynthetic capacity of improved rice plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114002194A true CN114002194A (en) | 2022-02-01 |
| CN114002194B CN114002194B (en) | 2023-07-21 |
Family
ID=79923769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111232782.8A Active CN114002194B (en) | 2021-10-22 | 2021-10-22 | Identification method for photosynthetic capacity of improved rice plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114002194B (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004017700A (en) * | 2002-06-12 | 2004-01-22 | Railway Technical Res Inst | Self-propelled electric traction inspection device |
| CN101584291A (en) * | 2009-07-03 | 2009-11-25 | 华中农业大学 | Method for detecting high-temperature stability of photosynthetic capacity of paddy rice |
| US20120018356A1 (en) * | 2009-03-06 | 2012-01-26 | Hendrik Jalink | Title of invention method and device for determining plant material quality using images containing information about the quantum efficiency and the time response of the photosynthtic system |
| KR101348077B1 (en) * | 2012-08-14 | 2014-01-03 | 사단법인대기환경모델링센터 | Automatic air collection chamber for the measurement of leaf respiration and photosynthesis rate of plants |
| CN103675247A (en) * | 2013-12-16 | 2014-03-26 | 贵州省亚热带作物研究所 | Whole tissue culture seedling photosynthetic rate rapid determining device and method |
| CN104359908A (en) * | 2014-10-30 | 2015-02-18 | 中国农业大学 | Method for measuring photosynthetic rate of CAM plants |
| CN107507192A (en) * | 2017-07-31 | 2017-12-22 | 上海交通大学 | A kind of Flag Leaves in Rice angle is in bulk measurement portable unit and in bulk measurement method |
| CN108871561A (en) * | 2018-04-25 | 2018-11-23 | 南京大学 | A kind of method of continuous observation rice canopy absorbed photosynthtic active radiation |
| CN109900643A (en) * | 2019-03-14 | 2019-06-18 | 石河子大学 | The rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate |
| CN110321627A (en) * | 2019-06-28 | 2019-10-11 | 西北农林科技大学 | Merge the photosynthetic rate prediction technique of leaf photosynthesis potential |
| CN210923438U (en) * | 2019-07-10 | 2020-07-03 | 贵州民族大学 | General type photosynthetic rate survey device of plant |
| GB202012952D0 (en) * | 2020-08-19 | 2020-09-30 | Univ Tartu | Method and apparatus for assessing gas exchange of plants |
| CN212514512U (en) * | 2020-09-11 | 2021-02-09 | 中南民族大学 | Portable plant photosynthetic rate detector |
| CN113063902A (en) * | 2021-04-25 | 2021-07-02 | 湖南杂交水稻研究中心 | Portable analysis system for field real-time determination of plant photosynthesis |
-
2021
- 2021-10-22 CN CN202111232782.8A patent/CN114002194B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004017700A (en) * | 2002-06-12 | 2004-01-22 | Railway Technical Res Inst | Self-propelled electric traction inspection device |
| US20120018356A1 (en) * | 2009-03-06 | 2012-01-26 | Hendrik Jalink | Title of invention method and device for determining plant material quality using images containing information about the quantum efficiency and the time response of the photosynthtic system |
| CN101584291A (en) * | 2009-07-03 | 2009-11-25 | 华中农业大学 | Method for detecting high-temperature stability of photosynthetic capacity of paddy rice |
| KR101348077B1 (en) * | 2012-08-14 | 2014-01-03 | 사단법인대기환경모델링센터 | Automatic air collection chamber for the measurement of leaf respiration and photosynthesis rate of plants |
| CN103675247A (en) * | 2013-12-16 | 2014-03-26 | 贵州省亚热带作物研究所 | Whole tissue culture seedling photosynthetic rate rapid determining device and method |
| CN104359908A (en) * | 2014-10-30 | 2015-02-18 | 中国农业大学 | Method for measuring photosynthetic rate of CAM plants |
| CN107507192A (en) * | 2017-07-31 | 2017-12-22 | 上海交通大学 | A kind of Flag Leaves in Rice angle is in bulk measurement portable unit and in bulk measurement method |
| CN108871561A (en) * | 2018-04-25 | 2018-11-23 | 南京大学 | A kind of method of continuous observation rice canopy absorbed photosynthtic active radiation |
| CN109900643A (en) * | 2019-03-14 | 2019-06-18 | 石河子大学 | The rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate |
| CN110321627A (en) * | 2019-06-28 | 2019-10-11 | 西北农林科技大学 | Merge the photosynthetic rate prediction technique of leaf photosynthesis potential |
| CN210923438U (en) * | 2019-07-10 | 2020-07-03 | 贵州民族大学 | General type photosynthetic rate survey device of plant |
| GB202012952D0 (en) * | 2020-08-19 | 2020-09-30 | Univ Tartu | Method and apparatus for assessing gas exchange of plants |
| CN212514512U (en) * | 2020-09-11 | 2021-02-09 | 中南民族大学 | Portable plant photosynthetic rate detector |
| CN113063902A (en) * | 2021-04-25 | 2021-07-02 | 湖南杂交水稻研究中心 | Portable analysis system for field real-time determination of plant photosynthesis |
Non-Patent Citations (2)
| Title |
|---|
| 张耀文 等: "作物高光效种质筛选的研究进展", 《中国农学通报》, vol. 35, no. 18 * |
| 郑巨云 等: "新疆陆地棉遗传连锁图谱构建及叶绿素含量和光合速率的QTL定位", 《新疆农业科学》, vol. 51, no. 9 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114002194B (en) | 2023-07-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103542811B (en) | Retainer automatically-detecting device | |
| CN202387677U (en) | Automatic collecting and sorting machine with scanning and detecting functions for voltage and internal resistance of lithium battery finished product | |
| CN109211075A (en) | A kind of plate roughness detection equipments | |
| CN214095862U (en) | Engineering geological crack measuring device | |
| CN114002194A (en) | Method for identifying photosynthetic capacity of improved rice plant | |
| CN114002382A (en) | Method for detecting high-temperature stability of rice photosynthetic capacity | |
| CN102091697A (en) | Product detection and sorting device for detecting aperture and sorting unqualified products | |
| CN113658796A (en) | Automatic winding equipment and winding method for network transformer production | |
| CN117250113A (en) | Building material strength detection equipment and application method thereof | |
| CN215815596U (en) | Automatic winding equipment is used in network transformer production | |
| CN221038590U (en) | Laser particle size distribution tester | |
| CN111912653A (en) | A sampling device is peeled off in layering for soil analysis | |
| CN220982992U (en) | Pericarp hardness tester suitable for fresh corn | |
| CN215065409U (en) | Continuous detection device of soil nutrient | |
| CN213933398U (en) | Rock-soil bearing capacity testing device | |
| CN216082277U (en) | High polymer material wearability detection device | |
| CN215262805U (en) | Anti detection anchor clamps of rolling over of concrete product | |
| CN113447296B (en) | Intelligent quantitative sampling device suitable for ellipsoidal fruits | |
| CN216668508U (en) | Rice plant height measuring device | |
| CN211262181U (en) | Integrated equipment for online detection of leather thickness | |
| CN219641539U (en) | Cloth friction color fastness detector | |
| CN219474574U (en) | BOPP film thickness detection device | |
| CN220789229U (en) | Cloth edge cutter | |
| CN218271706U (en) | Tensile detection device of sticky tape | |
| CN209618517U (en) | A kind of cutting lap former for exterior-wall heat insulation grid cloth |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220318 Address after: 241000 building A12, Service Outsourcing Industrial Park, Wuhu high tech Industrial Development Zone, Anhui Province (No. 717, south Zhongshan Road) Applicant after: YIELEAD RICE INDUSTRY CO.,LTD. Applicant after: Anhui Yuanliang Seed Industry Research Institute Address before: 241000 building A12, Service Outsourcing Industrial Park, Wuhu high tech Industrial Development Zone, Anhui Province (No. 717, south Zhongshan Road) Applicant before: YIELEAD RICE INDUSTRY CO.,LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |