CN109900643A - The rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate - Google Patents
The rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate Download PDFInfo
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- CN109900643A CN109900643A CN201910195243.8A CN201910195243A CN109900643A CN 109900643 A CN109900643 A CN 109900643A CN 201910195243 A CN201910195243 A CN 201910195243A CN 109900643 A CN109900643 A CN 109900643A
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Abstract
The present invention relates to the experimental facilities that plant photosynthetic rate and transpiration rate measure.A kind of rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate, including plant photosynthesis analyzer host, the sample room IRGA of IRGA analyzer, tufted leaf chamber mounting plate, the sample room IRGA of the IRGA analyzer removes cover board, it is connect with tufted leaf chamber mounting plate, self-control leaf chamber is installed on the tufted leaf chamber mounting plate, the connecting plate is equipped with venthole, by the venthole, so that the self-control leaf chamber, tufted leaf chamber mounting plate are connected with the sample room IRGA.The configuration of the present invention is simple, capacity be suitable for, reliable operation, the single fruit branch of cotton photosynthetic rate in its natural state and transpiration rate can accurately, easily be monitored, the photosynthetic rate and transpiration rate that the single or multiple organs of other plants can also be measured are a kind of measurement devices that is economical and practical, being suitable for popularization.
Description
Technical field
The present invention relates to the experimental facilities that plant photosynthetic rate and transpiration rate measure, and especially one kind is with the single fruit of cotton
Branch is the photosynthetic rate that the bell leaf system of object is united and the device that transpiration rate quickly measures.
Background technique
Photosynthetic rate refers to the rate of the fixed carbon dioxide of plant, the i.e. carbon dioxide fixation of unit time unit leaf area
(or oxygen release) amount.Situations such as photosynthetic rate index can react the photosynthetic capacity of plant, whether plant source and sink relation is coordinated,
At present there are mainly two types of measurement methods: single leaf is photosynthetic and population photosynthesis measures.But cotton is different from the single grass family in source library
Class crop, the more libraries in the source of cotton are more, and corresponding relationship is complicated.Single photosynthetic measurement of leaf can only react the photosynthetic of surveyed vane type source
Ability, and population photosynthesis measures, mainly by Photo distribution in population, reacton land area biomass accumulation and yield
Situation, single photosynthetic measurement of leaf is with population photosynthesis measurement since institute's measurement ruler degree differs greatly, it is difficult to react the light of single plant difference fruit branch
Whether conjunction ability and source and sink relation are coordinated.In the prior art, lack cotton fruit branch photosynthetic rate and transpiration rate quickly measures
Device, existing equipment can not improve the efficiency and accuracy of DATA REASONING.
Summary of the invention
The purpose of the present invention is to provide a kind of structurally reasonable, capacity to be suitable for, is economical and practical, and measurement effect is good, is able to achieve
By the quick measurement of the photosynthetic rate and transpiration rate of the single fruit branch of cotton or multiple organs, cotton can be accurately and easily monitored
Fruit branch or multiple organs photosynthetic rate in its natural state and transpiration rate, can be improved the efficiency and accuracy of DATA REASONING
Cotton bell leaf system system photosynthetic rate and transpiration rate rapid determination device.
The invention discloses a kind of rapid determination devices of cotton bell leaf system system photosynthetic rate and transpiration rate, mainly include
Plant photosynthesis analyzer host, the sample room IRGA of IRGA analyzer, tufted leaf chamber mounting plate, the IRGA of the IRGA analyzer
Sample room is connected by cable with plant photosynthesis analyzer host, it is characterised in that the IRGA sample of the IRGA analyzer
Room removes cover board, connect with tufted leaf chamber mounting plate, self-control leaf chamber, the connection are equipped on the tufted leaf chamber mounting plate
Plate is equipped with venthole, by the venthole, so that the self-control leaf chamber, tufted leaf chamber mounting plate are connected with the sample room IRGA
It is logical；Per se with circular hole on tufted leaf chamber mounting plate 10, venthole here is communicated with circular hole on tufted leaf chamber mounting plate 10.
The self-control leaf chamber is rectangular hollow box body, including upper cartridge body, back box lid, upper cartridge body top surface are that light transmittance is 85%-
90% transparent membrane is made, and bottom is the opening-closing structure being made of back box lid, hinge, upper cartridge body bottom, the upper cartridge body with
Back box lid opening and closing place is equipped with lock, is equipped with lower notch at the hole edge of back box lid, upper cartridge body hole edge is under
The corresponding position of notch is equipped with upper notch；In upper cartridge body be equipped with fan, the upper cartridge body side wall be equipped with matching tube, described
It is piped the other end and connects the sample room IRGA, the self-control leaf chamber and the sample room IRGA are connected to by the matching tube.
Preferably, the hole edge of the back box lid and upper cartridge body is all provided with spring seal strip, the upper notch, under
Notch is recess, the upper notch, lower notch recess in fill spring seal strip.
Preferably, the self-control leaf chamber is made of transparent material, the volume for making leaf chamber by oneself is greater than 2L.
Preferably, the connecting plate specification is 5cm × 5cm, with a thickness of 5mm；Make by oneself leaf chamber specification be 25.4 ×
25.4 × 6cm, material is transpex plate, with a thickness of 5mm.
Preferably, the power of the fan is 1.80W, size 5cm × 5cm, with a thickness of 1cm, revolving speed 6500
R.P.M, maximum 0.384 m3/min of wind flow.
Preferably, the IRGA sample of plant photosynthesis analyzer host model LI-6400XT, the IRGA analyzer
Room, tufted leaf chamber mounting plate, matching tube, the standard fitting that cable is LI-6400XT.
When work, CO2 and H2O are monitored with IRGA (that is: infrared gas analyser), need to not change growth rate of cotton plant shape
In the case where state, organs single or multiple in plant are covered together in self-control leaf chamber, the inspection work before preheating and after preheating
And the measurement of photosynthetic rate and transpiration rate, operated according to the detection mode of plant photosynthesis analyzer.Cotton is single
Fruit branch photosynthetic rate and transpiration rate rapid detection method are to cover entire fruit branch in self-control leaf chamber together, before preheating and pre-
Inspection work and the measurement of photosynthetic rate after heat, and operated by the detection mode of plant photosynthesis analyzer.
Working principle of the present invention:
The rapid determination device of cotton bell leaf system provided by the invention system photosynthetic rate and transpiration rate, incorporate single leaf it is photosynthetic and
The advantages of population photosynthesis measures, can accurately and easily measure the photosynthetic capacity of the single fruit branch of cotton, and it is single can also to measure other plants
The photosynthetic rate and transpiration rate of a or multiple organs improve the efficiency and accuracy of DATA REASONING.
The present invention improves existing plant photosynthesis analyzer, substitutes tufted leaf chamber using self-control leaf chamber, can
Accurately and easily monitoring plant photosynthetic rate in its natural state and transpiration rate, and the single or multiple organs of plant can be measured
Photosynthetic rate and transpiration rate；The volume for making leaf chamber by oneself is 2L or more, solves tufted leaf chamber and can not measure area and is greater than
The problem of the plant organ of 10cm2.
By adopting the above-described technical solution, compared with prior art, the present invention can be directly to the single fruit branch of cotton
It is measured as a whole, and can be research by stem leaf, fruit branch leaf, bract, the bell and combinations thereof in single fruit branch
Object is measured photosynthetic rate and transpiration rate；According to plant photosynthesis analyzer according to sample room and reference cell CO2, H2O gas
The difference of the scale of construction calculates the working principle of photosynthetic rate and transpiration rate.
Advantages of the present invention: the configuration of the present invention is simple, capacity be suitable for, reliable operation, can accurately, easily monitor cotton list
A fruit branch photosynthetic rate in its natural state and transpiration rate, can also measure the photosynthetic speed of the single or multiple organs of other plants
Rate and transpiration rate can be improved the efficiency and accuracy of DATA REASONING, be a kind of cotton bell leaves that is economical and practical, being suitable for popularization
The rapid determination device of system photosynthetic rate and transpiration rate.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the embodiment of the present invention 1.
Fig. 2 is the structural schematic diagram for making the leaf chamber back side in Fig. 1 by oneself.
Fig. 3 is the structural schematic diagram for making leaf chamber side in Fig. 1 by oneself.
Fig. 4 is the structural schematic diagram made by oneself before leaf chamber in Fig. 1.
Fig. 5 is the structural schematic diagram that self-control leaf chamber bottom surface is looked up in Fig. 1.
Fig. 6 is the upper cartridge body for making leaf chamber in Fig. 1 by oneself and the partial enlargement structural representation under back box lid opening state.
As shown in the figure: 1 is plant photosynthesis analyzer host, and 2 be the sample room IRGA of IRGA analyzer, and 3 be fan, and 4 are
Make leaf chamber by oneself, 5 be upper notch, and 6 be upper cartridge body, and 7 be lower notch, and 8 be back box lid, and 9 be matching tube, and 10 install for tufted leaf chamber
Plate, 11 be connecting plate, and 12 be venthole, and 13 be sealing strip, and 14 be lock, and 15 be hinge, and 16 be upper cartridge body bottom, and 17 be cable
Line, 18 are upper cartridge body top surface.
Specific embodiment
Embodiment 1:
Referring to Fig.1-Fig. 6 is structural schematic diagram of the embodiment of the present invention, a kind of cotton bell leaf system system photosynthetic rate and transpiration rate
Rapid determination device, mainly include plant photosynthesis analyzer host 1, the sample room IRGA 2 of IRGA analyzer, tufted leaf chamber peace
The sample room IRGA 2 of loading board 10, the IRGA analyzer is connected by cable with plant photosynthesis analyzer host, feature
It is the sample room the IRGA removal cover board of the IRGA analyzer, is connect with tufted leaf chamber mounting plate 10, in the tufted leaf chamber
Self-control leaf chamber 4 is installed, the connecting plate 11 is equipped with venthole, by the venthole, so that the self-control on mounting plate 10
Leaf chamber 4, tufted leaf chamber mounting plate 10 are connected with the sample room IRGA 2；Per se with circular hole on tufted leaf chamber mounting plate 10, this
In venthole communicated with circular hole on tufted leaf chamber mounting plate 10.
The self-control leaf chamber 4 is rectangular hollow box body, including upper cartridge body 6, back box lid 8, and upper cartridge body top surface 18 is light transmittance
It being made of the transparent membrane of 85%-90%, bottom is the opening-closing structure being made of back box lid 8, hinge 15, upper cartridge body bottom 16,
The upper cartridge body 6 is equipped with lock 14 with 8 opening and closing place of back box lid, is equipped with lower notch 7 at the hole edge of back box lid 8, it is described on
6 hole edge of box body position corresponding with lower notch 7 is equipped with upper notch 5；Fan 3, the upper box are equipped in upper cartridge body 6
6 side wall of body is equipped with matching tube 9, and 9 other end of matching tube connects the sample room IRGA 2, by the matching tube 9 be connected to it is described from
Leaf chamber 4 processed and the sample room IRGA 9.
The hole edge of the back box lid 8 and upper cartridge body 6 is all provided with spring seal strip 13, the upper notch 5, lower notch
7 be recess, the upper notch 5, lower notch 7 recess in fill spring seal strip 13.
11 specification of connecting plate is 5cm × 5cm, with a thickness of 5mm；The specification for making leaf chamber 4 by oneself is 25.4 × 25.4
× 6cm, with a thickness of 5mm, material is transpex plate.
The power of described two fans 3 is 1.80W, size 5cm × 5cm, with a thickness of 1cm, revolving speed 6500
R.P.M, maximum 0.384 m3/min of wind flow.
1 model LI-6400XT, the IRGA analyzer 2 of plant photosynthesis analyzer host, matching tube 9, cable 17 are
The standard fitting of LI-6400XT.
In the use of the present invention, the contact surface up and down when upper cartridge body 6, back box lid 8 are closed, is ensured using elastic sealing strip 13
The airtightness for making leaf chamber by oneself can be used as after fruit branch is put into self-control leaf chamber 4, that gives is further close with 14 fastening of butterfly lock
The external force of envelope is additionally provided with the upper and lower notch of fixed fruit branch in self-control leaf chamber 4, fills spring seal strip in upper and lower notch
13, after fruit branch is put into, upper cartridge body 6, back box lid 8 are covered tightly, then fruit branch is pressed in upper and lower notch, utilizes elastic sealing strip
The gap for filling fruit branch, plays the role of sealing；Two fans 3 are also mounted in self-control 4 medial surface of leaf chamber, for mixing self-control leaf
Gas in room 4；Self-control leaf chamber 4 is connect by connecting plate 11 with 2 rigid seal of IRGA analyzer, and self-control leaf chamber 4 passes through venthole
12 are connected with IRGA analyzer 2；IRGA analyzer 2 is connect by data cable 17 with plant photosynthesis analyzer host 1；
4 entirety of self-control leaf chamber is the container of a cuboid, and the upper and lower notch 5,7 of fixed fruit branch is having a size of 2 × 1cm.
By taking the measuring process of non-controlling environmental condition as an example:
1, chemicals is installed, is connected hardware (if using CF card, be inserted into behind host and fix in sulculus).Booting, configuration
Interface selects Factory default, and connection status presses " Y ", and into main menu, flow velocity selects 700 μm of ol/s, and preheating is about
20min；
2, current check is carried out, without closing air mixing fan when carrying out matching operation；
3, self-control leaf chamber is opened, puts cotton fruit branch or its organ into；
4, F1 (Open Log File) is pressed, selects the position (host or CF card) for being stored in data, establishes a file,
Enter inputs a remark, enter；
5, a row parameter stability is waited, b row Δ CO2 value fluctuates < 0 ~ 2 μm of ol mol-1, Photo parameter stabilities in decimal point
Latter position, c row parameter in the normal range (Tr > 0), by F1 (Log) record data；
6, replacement measurement sample adds remark by F4, repeats 3~5 steps, measures.It need to be carried out when replacing sample
Match；
7, F3 (Close file) is pressed, saves data file；
8, computer and LI-6400XT are connected with RS-232 data line, retracts main interface by esc, by F5 (Utility Menu),
" File Exchange Mode " is selected by down arrows, installation SimFX software, double-click are opened in advance on computers
L16400FileEx clicks File, selects Prefs, selects the port Com, by Connect, after successful connection, and select file transmission
To designated position (data in CF card can also be inserted directly into computer card reader after exiting card to export data)；
9, esc is pressed, main interface is retracted, is shut down；
10, chemical tube knob is threaded to intermediate slack state；Self-control leaf chamber lock is opened, self-control leaf chamber is kept to be in open shape
State.
Claims (7)
1. a kind of rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate, mainly includes plant photosynthesis analyzer
Host, the sample room IRGA of IRGA analyzer, tufted leaf chamber mounting plate, the sample room IRGA of the IRGA analyzer pass through cable
Line is connected with plant photosynthesis analyzer host, it is characterised in that and the sample room IRGA of the IRGA analyzer removes cover board, with
The connection of tufted leaf chamber mounting plate, is equipped with self-control leaf chamber on the tufted leaf chamber mounting plate, and the connecting plate is equipped with ventilation
Hole, by the venthole, so that the self-control leaf chamber, tufted leaf chamber mounting plate are connected with the sample room IRGA；
The self-control leaf chamber is rectangular hollow box body, including upper cartridge body, back box lid, upper cartridge body top surface are that light transmittance is 85%-90%
Transparent membrane be made, bottom is the opening-closing structure being made of back box lid, hinge, upper cartridge body bottom, the upper cartridge body and bottom
Box cover opening and closing place is equipped with lock, is equipped with lower notch at the hole edge of back box lid, upper cartridge body hole edge is lacked with lower
The corresponding position of mouth is equipped with upper notch；Fan is equipped in upper cartridge body, the upper cartridge body side wall is equipped with matching tube, the matching
The pipe other end connects the sample room IRGA, is connected to the self-control leaf chamber and the sample room IRGA by the matching tube.
2. the rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate as described in claim 1, feature exist
It is all provided with spring seal strip in the hole edge of the back box lid and upper cartridge body, the upper notch, lower notch are recess, institute
It states in the recess of notch, lower notch and fills spring seal strip.
3. the rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate as claimed in claim 1 or 2, feature
It is that the self-control leaf chamber is made of transparent material, the volume for making leaf chamber by oneself is greater than 2L.
4. the rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate as claimed in claim 3, feature exist
In the connecting plate specification be 5cm × 5cm, with a thickness of 5mm；The specification for making leaf chamber by oneself is 25.4 × 25.4 × 6cm, material
Matter is transpex plate, with a thickness of 5mm.
5. the rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate as claimed in claim 3, feature exist
It is 1.80W, size 5cm × 5cm in the power of the fan, with a thickness of 1cm, revolving speed is 6500 R.P.M, maximum wind flow
0.384 m3/min。
6. the rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate as claimed in claim 4, feature exist
It is 1.80W, size 5cm × 5cm in the power of the fan, with a thickness of 1cm, revolving speed is 6500 R.P.M, maximum wind flow
0.384 m3/min。
7. the rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate as claimed in claim 6, feature exist
It is installed in the sample room IRGA of plant photosynthesis analyzer host model LI-6400XT, the IRGA analyzer, tufted leaf chamber
Plate, matching tube, the standard fitting that cable is LI-6400XT.
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| CN201910195243.8A CN109900643A (en) | 2019-03-14 | 2019-03-14 | The rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate |
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| CN201910195243.8A CN109900643A (en) | 2019-03-14 | 2019-03-14 | The rapid determination device of cotton bell leaf system system photosynthetic rate and transpiration rate |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111766239A (en) * | 2020-06-22 | 2020-10-13 | 华中农业大学 | Potted plant photosynthetic rate high-throughput measuring robot |
| CN114002194A (en) * | 2021-10-22 | 2022-02-01 | 安徽袁粮水稻产业有限公司 | Method for identifying photosynthetic capacity of improved rice plant |
| CN114814104A (en) * | 2022-04-28 | 2022-07-29 | 湖南杂交水稻研究中心 | Device for inducing photosynthesis of single-sided leaves |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111766239A (en) * | 2020-06-22 | 2020-10-13 | 华中农业大学 | Potted plant photosynthetic rate high-throughput measuring robot |
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| CN114002194A (en) * | 2021-10-22 | 2022-02-01 | 安徽袁粮水稻产业有限公司 | Method for identifying photosynthetic capacity of improved rice plant |
| CN114002194B (en) * | 2021-10-22 | 2023-07-21 | 安徽袁粮水稻产业有限公司 | Identification method for photosynthetic capacity of improved rice plants |
| CN114814104A (en) * | 2022-04-28 | 2022-07-29 | 湖南杂交水稻研究中心 | Device for inducing photosynthesis of single-sided leaves |
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