CN101584291A - Method for detecting high-temperature stability of photosynthetic capacity of paddy rice - Google Patents
Method for detecting high-temperature stability of photosynthetic capacity of paddy rice Download PDFInfo
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Abstract
The invention belongs to the technical field of crop breeding, specifically relates to a method for sieving high-temperature adaptive resources of paddy rice and for evaluating and authenticating high-temperature stability of photosynthetic capacity of breeding material. The invention comprises the characteristics of: adjustment for the heading stage of the material to be authenticated via seeding period, selection for natural conditions of photosynthetic rate determination, confirmation for growth period and determination time of paddy rice, setting of parameter values of artificial light and temperature, processing and analysis of data and the like. During the specific period for growth and development of the paddy rice, conditions including natural high temperature in summer and artificial light and temperature are utilized to implement definite-time and constant-temperature determination on net photosynthetic rate of paddy rice leaf, and the obtained result can relatively accurately analyze differences of photosynthesis among testing varieties of the paddy rice in high-temperature stability. The invention provides convenient and feasible evaluation indicators and methods for the selection of parent and the sieving of breeding progeny in high-temperature adaptive ecological breeding and in high photosynthetic efficiency physiological breeding.
Description
Technical field
The invention belongs to new crop varieties breeding technique field, relevant with crop physiology and crop ecology, the present invention relates to a kind of method for testing and analyzing of paddy rice ecological breeding correlation technique index, particularly, the detection method that relates to a kind of high-temperature stability of the photosynthetic capacity to rice breed.
Background technology
Paddy rice is a staple food crop in the world, short bar genetic resources of paddy rice and heterotic utilization make rice yield obtain continuous breakthrough, but the light energy of rice availability of cultivation is still also lower at present, improves rice yield by the photosynthetic efficiency that improves paddy rice and still has bigger potentiality.Because sustainable growth, the per capita area of cultivated farmland of China's population constantly reduce and the deficiency of water resource, satisfy the grain demand of China, significantly increasing on the paddy rice cultivated area difficulty, so the raising rice yield is a main target from now on, improving utility rate of luminous energy is one of effective way that improves rice yield.High light efficiency rice breeding is the important directions of paddy rice physiology breeding, is the element task of carrying out selection technology in the physiology breeding to the physiology evaluation of breeding progeny material and the foundation of assessment indicator system.
The obvious aggravation in the whole world " greenhouse effect " makes the high temperature adverse circumstance be subjected to extensive concern to the influence of rice yield and quality.Blade is that paddy rice carries out photosynthetic major organs, its high photosynthetic efficiency is the basis that guarantees the paddy rice carbon assimilation, improves utility rate of luminous energy, have data to show, the photosynthetic capacity of back 3 functional leafs plays an important role to seed circularity and quality in growth period duration of rice.From environmental factor, temperature and illumination are the photosynthetic main ecological conditions of influence, existing rice varieties photosynthetic rate under summer afternoon high temperature or high light can take place obviously to descend to some extent, be called " photosynthetic afternoon nap " phenomenon, reduced the synthetic and accumulation efficient of light-use and organic matter, bigger to the Rice Production influence of torrid areas and China middle and lower reach of Yangtze River and south China Dao Qu.
The kind of high photosynthesis efficiency should possess more stable photosynthetic capacity, and its photosynthetic rate shows relative insensitiveness to high temperature or high light, and the light that photosynthetic " afternoon nap " occurs, warm threshold value are than general kind height.Therefore, the light temperature threshold value that " afternoon nap " occur just can be used as a physiological standard estimating the rice varieties photosynthetic capacity, can be used for high light efficiency rice breeding offspring's screening and evaluation.High-temperature adaptability combined with the breeding of high photosynthetic efficiency to seek a kind of selection index of estimating the rice high yield stable yields be the content that lacks research at present.In high light efficiency physiology breeding work, require a large amount of breeding resources and progeny material are carried out fast, accurately identified.Therefore needing to explore some indirect proterties and indexs more directly related with photosynthesis characteristics, that be easier to accurately measure is very important basic technology.The method that does not also have at present a cover simply to be suitable for is applied to the evaluation method to paddy rice photosynthesis high-temperature stability.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, propose a kind of detection method of the high-temperature stability to photosynthetic capacity of paddy rice.Applicant's inventor has carried out the research of several years for this reason, proposes a cover paddy rice resource and breeding offspring are carried out index and the authentication method that the photosynthetic capacity temperature stability is estimated.By more than 30 parts of rice varieties commonly used, male sterile line and recovery system are carried out photosynthesis diurnal variation dynamic change observation under the high temperature, find that all leaf photosynthesis speed for the examination material reach higher value in the morning about 9:00, tangible decline has all taken place under the high temperature high light condition in noon, and photosynthetic " afternoon nap " phenomenon is ubiquity in for the rice varieties that tries the water.The degree of different materials photosynthetic " afternoon nap " phenomenon is not quite similar, but its photosynthetic minimum point mostly occurs about 13:30.The inventor carries out significance test with the difference of the photosynthetic rate value of these two time points, can draw the significance degree of different materials drop-out value.This difference is performance stability preferably between year and between repeating, and therefore photosynthetic drop-out value can be used as one of the evaluation index of the high-temperature stability difference of photosynthetic capacity of paddy rice.The photosynthetic diurnal variation of the rice material of several differential responses types as shown in Figure 5.
Analyze leaf temperature, air and intercellular space CO
2The diurnal variation of the factors such as concentration, air humidity, stomatal conductance, leaf temperature, light intensity, stomatal conductance and photosynthesis diurnal variation have the uniformity variation tendency as can be seen, air humidity and CO
2Concentration is not main affecting factors under the paddy rice water condition.Stomatal conductance is the plant physiology adaptability protective reaction that high temperature and high light cause.
The applicant is further in the artificial growth case and phytotron of intensity of illumination unanimity, under serial condition of different temperatures, measure photosynthetic rate (seeing embodiment), find that rice leaf surface temperature single-factor also can cause the decline of photosynthetic value, different materials has different photosynthetic letdown point temperature.Therefore, can be used as one of index of estimating rice leaf photosynthetic performance high-temperature stability with photosynthetic difference and photosynthetic letdown point temperature value.
Particularly, technical scheme of the present invention is as follows:
A kind of detection method to the photosynthetic capacity of paddy rice high-temperature stability, its step comprises:
Photosynthetic stability under A, the natural high-temperature high light condition is identified
(1) plantation of mensuration material: the rice material of test usefulness is according to determining its sowing time its breeding time, early rice was sowed in about the May 25 in every year in rice district, the Chinese middle and lower reach of Yangtze River, in, late rice sows in about the May 10 in every year, make to be in annual July 25 its heading stage between August 15; The 4-5 leaf is transplanted during the phase in the land for growing field crops, 2 sub-districts of every part of material plantation, every district plantation 4 row totally 40 strains; Normal rich water quality management;
(2) grouping of measuring material with decide strain: according to test material by breeding time mutually approximately principle be divided into a plurality of test group, every group of test material is no more than 10 parts, require test paddy rice population growth unanimity, and surveyed the chlorophyll content value (SPAD value) of each plant stem sword-like leave before the phase with the chlorophyll measuring instrument in the beginning fringe, select in the sub-district the most approaching individual plant of SPAD value to decide strain 6 strains and number, as after carry out photosynthetic rate mensuration individual plant (see figure 1);
(3) photosynthetic rate is measured: in the week, select temperature to be higher than 33 ℃ fine day behind the beginning fringe, 9:00 begins the sword-like leave of selected rice plant is carried out photosynthetic rate mensuration the morning of every day, and measuring the position is 1/3 place, front portion of sword-like leave blade; 1 minute each determination data time spent; Every group of material measured in 1 hour in limited time and finished; 13:00 on the same day begins the repetition mensuration in the morning; Repeating 2 times again in the week age of back measures; Photosynthetic mensuration system is the Li-co produced in USA 6400 portable photosynthetic rate determination instrument of separate unit or the portable photosynthetic rate determination instrument (see figure 2) of CIRAS-I (II) type that Britain PPS company produces, or adopt the coupling of many portable photosynthetic rate determination instrument composition measuring systems, make this instrument can measure the leaf temperature automatically, stomatal conductance, intercellular CO
2Concentration, environmental temperature, humidity parameter;
(4) interpretation of result with assay: test branchs sub-district and different mensuration inferior (my god) number scale records data, determination data imports computer into and is converted to Office Excel tables of data, get 6 strain measurement result calculating mean values, analyze data stability between repetition, the stomatal conductance that obtains simultaneously during with mensuration, leaf temperature parameter is analyzed the reasonability of photosynthetic value, to irrational test value repetition measurement once or reject.
(5) particularity of different materials colony is handled: the primary election of breeding progeny material, can not establish repetition, colony finished in 2 hours, and can measure 120 strains every day, and according to the difference at heading stage, whole colony can finish mensuration in several days.For close more material breeding time, interval sowing is adjusted to and carries out different heading stages after the packets available.The evaluation work that surpasses above-mentioned experimental scale is finished in the portable photosynthetic rate determination instrument system coupling of available separate unit or 2 or many compositions (the portable photosynthetic rate determination instrument of CIRAS-I (II) type that for example above-mentioned Li-co produced in USA 6400 portable photosynthetic rate determination instrument or Britain PPS company produce, the similar products that also can adopt other countries to produce); Or
The mensuration of photosynthetic rate letdown point temperature under B, the artificial illumination and temperature condition
(1) plantation of test material: employing is the breeding time of the method adjusting rice plant of sowing in batches, and the rice material for examination is separated heading stage; The rice plant of participating in the experiment was transplanted to cultivating alms bowl in 5 leaf phases, every material 5 alms bowls of planting, the chlorophyll content value (SPDA value) of sword-like leave is measured in every potting 3 strains with the chlorophyll measuring instrument before the beginning fringe, select the individual plant of SPAD value unanimity to decide strain and carry out photosynthetic mensuration;
(2) artificial illumination and temperature condition setting: select large-scale climatic chamber of the formula of stepping into or growth case for use, the cultivation alms bowl of heading was put into artificial illumination and temperature growth case or climatic chamber the same day of test SPDA value, adopt Biological lamp illumination, the setting light intensity is 20000lx, adapting to 2 hours laggard trip temperatures of illumination regulates, the temperature setting is warming up to 38 ℃ by 28 ℃, and the rice leaf temperature is changed in 30 ℃-42 ℃, and the temperature of the above evenly rose in 3 hours;
(3) photosynthetic rate is measured: in the temperature uphill process, photosynthetic mensuration is carried out once with interior in every interval 20 minutes; 2 parts of rice materials of each mensuration, each testing of materials 3 strain was also finished in 5 minutes, and statistical data is averaged.2 materials hocket, and the peak that reaches setting to leaf temperature is to finish more than 42 ℃ to measure;
(4) data analysis: be converted to the OfficeExcel tables of data and draw leaf table actual temperature and the photosynthetic rate relation curve with the photosynthetic rate of analyzer acquisition and the test data values of blade actual temperature, find out the photosynthetic corresponding leaf table temperature value that descends of every part of test material.With photosynthetic letdown point temperature is to be that responsive to temperature type material, 35 ℃-37 ℃ are medium sensitivity section bar material below 35 ℃, is that the high-temperature stable material is as evaluation criterion more than 37 ℃.
In the present invention, wherein the described assay method of step (1) also is included in the fine high-temperature stability index of estimating photosynthetic capacity of paddy rice with the difference of the rice leaf Net Photosynthetic Rate that 9:30 and 13:30 measured of high temperature.
Wherein the described assay method of step (2) also comprise utilize temperature that artificial illumination and temperature growth case or artificial illumination and temperature climatic chamber be provided with in limited time between in 3 hours by the parameter setting of 28 ℃ of even variation to 38 ℃, the intensity of illumination parameter is provided with, and estimates the standard of paddy rice high-temperature stability power with photosynthetic rate letdown point temperature.
Can analyze different materials photosynthesis high-temperature stability feature under field conditions (factors) to the steps A determination data, be mainly used in Preliminary screening variety source and breeding offspring segregation population; Step B appraising datum is used to analyze the photosynthetic beginning letdown point temperature of different materials, is primarily aimed at the photosynthesis heat-resisting quantity analysis of kind, advanced lines stable strain and resource, and the result can be used as the foundation of determining kind Secure Application scope.
Description of drawings
Fig. 1: field paddy rice test material plantation situation (the requirement rice varieties growth regularity of participating in the experiment is good, leaf look unanimity between strain);
Fig. 2: photosynthesis measurement instrument CIRAS-1 used in the present invention (available from Britain, this instrument can be measured and parameters such as storage leaf temperature, air themperature, humidity, stomatal conductance, intercellular CO2 concentration simultaneously automatically);
Fig. 3: photosynthesis measurement method signal (fixed point is measured preceding 1/3 place of strain paddy rice sword-like leave);
Fig. 4: the artificial illumination and temperature of rice material is handled (it is big both to have had living space, characteristics such as illumination, uniformity of temperature profile);
Fig. 5: the photosynthetic diurnal variation situation of several parts of photosynthetic high-temperature stability rice varieties of difference.
Embodiment
Photosynthetic capacity high-temperature stability to 8 parts of rice material natural conditions following heading stages is measured
Be chosen in the upward representative dissimilar long-grained nonglutinous rices of production and recover system, the male sterile line kind is as part surplus examination material 30, identified through 2 years, filter out good rice breed " the another name for Sichuan Province extensive 527 " (source: the kind of Sichuan Agricultural University's seed selection) of photosynthetic high-temperature stability that meets breeding requirement of the present invention, (Hunan of " training short 64S ", the kind of Long Pinggaoke agricultural high-tech limited company seed selection) kind such as, wherein the material of the photosynthetic poor stability of high temperature has " special Xian accounts for 25 " (source: the seed selection of Fushan City, Guangdong Province research of agricultural science institute), " osmanthus 99 " (academy of agricultural sciences, Guangxi provides).The different materials differences 2 annual bearings formula unanimity that becomes, repeatability are better.
It is that example explanation method of testing and operating procedure are as follows that choosing wherein is divided into one group of 8 parts of material measuring:
(1) according to the characteristic in breeding time of each material, 8 parts of semilate rice materials are divided into one group, in sowing on May 10th, 2006, were transplanted into the land for growing field crops on June 5, repeat every material 2 sub-districts, 40 strains of every sub-district.
(2) measured stem sword-like leave chlorophyll SPAD value on August 6, every part of every sub-district of material selects 6 strains to decide strain as the photosynthesis measurement strain.
(3) select fine high temperature August 14 9:00 and 13:00 begin to carry out photosynthetic rate and measure.Be recorded as 9:30 and 13:30 respectively according to the elapsed time median.Photosynthetic mensuration system is the portable photosynthetic rate determination instrument (see figure 2) of CIRAS-I (II) type that Li-co 6400 portable photosynthetic rate determination instrument produced in USA or Britain PPS company produce, or can measure leaf temperature, stomatal conductance, intercellular CO automatically
2Concentration, the photosynthetic mensuration system that environmental temperature, the isoparametric separate unit of humidity or many portable photosynthetic rate determination instrument are formed (for example: the portable photosynthetic rate determination instrument of CIRAS-I (II) type that Britain PPS company produces, also can adopt other countries' similar products);
(4), the photosynthetic mean value of each material is listed in table 1 according to determination data:
Table 1. different materials photosynthetic rate is at decline difference (the μ molCO of 9:30 and 13:30
2m
-2s
-1)
* represent that difference reaches significance level (F
0.05=9.28, F
0.01=29.46)
(5) interpretation of result: special Xian accounts for 25, the photosynthetic rate drop-out value of continuous extensive 725,488 (488 are the rice material of Hua Zhong Agriculture University's seed selection) is big, belongs to the big type of photosynthetic decline degree, the photosynthetic good stability of other material.
Production application shows that " another name for Sichuan Province extensive 527 " is a good rice restorer, is a plurality of parents that pass through the hybrid combination of the above new varieties authorization of Chinese Provincial, produces wide adaptability, and is big at the popularizing area of China." training short 64S " is the important male sterile line of Chinese two-line hybrid rice, and what be made into by this male sterile line strong excellent two is that combination is totally authorized more than 20, applies area in China's accumulation and has reached more than 100,000,000 mu.It is a little that high-yield hybrid combination region bad adaptability is joined in continuous extensive 725 (seed selections of Sichuan Province's Mianyang City Academy of Agricultural Sciences), and special Xian accounts for 25 and is applicable to specific production area.The strong material of other stability remains to be applied and adaptive further confirmation.This sample result shows with method gained conclusion of the present invention and conforms to the production actual result.
Embodiment 2 (Application Example):
The photosynthesis stability difference is bigger under 2 parts of natural high-temperature conditions material " another name for Sichuan Province extensive 527 " and " special Xian accounts for 25 " are identified photosynthetic letdown point temperature under the artificial illumination and temperature condition.
(1) with 2 parts of materials, in sowing on May 10th, 2007, June 5 was transplanted in the potted plant alms bowl, totally 6 strains of every material 2 alms bowls.
(2) measured stem sword-like leave chlorophyll SPAD value on August 10, every part of material selects 3 strains to decide strain as the photosynthesis measurement strain.
(3) be provided with in large-sized artificial light temperature growth chamber from 28 ℃ of-38 ℃ of transformation temperatures, the temperature average rate rises between two temperature set points.Illumination degree of drawing 20000Lx remains unchanged.
In the table 2. climatic cabinate diesel locomotive hour by 28 ℃ of temperature settings that evenly are warming up to 38 ℃
(4) alternately mensuration of photosynthetic rate is carried out in each 3 strain to 2 parts of materials, guarantees per 20 minutes mensuration once, measures altogether 8 times.
(5) Net Photosynthetic Rate and the blade surface actual temperature value that record of photosynthetic mensuration system listed in table 2.Analysis learns that there is notable difference in the photosynthetic letdown point temperature of 2 parts of materials, and the photosynthetic letdown point temperature in " another name for Sichuan Province extensive 527 " is 38 ℃, and " it is 36 ℃ that special Xian accounts for 25.
This result can explain that under field conditions (factors) " another name for Sichuan Province extensive 527 " has stronger thermal adaptability, thereby shows than " special Xian accounts for 25 " the thermotonus insensitiveness under same high temperature.This result conforms to the result that embodiment 1 measures under field conditions (factors).
Variation (the μ molCO of paddy rice leaf photosynthetic rate under table 3. condition of different temperatures
2m
-2s
-1)
Claims (3)
1, a kind of detection method at the photosynthetic capacity of paddy rice high-temperature stability, its step comprises:
(1) under natural high-temperature high light condition to the detection of the high-temperature stability of photosynthetic capacity of paddy rice
1) measure the plantation of material: the rice material of test usefulness is according to its characteristic in breeding time different arrangement different sowing time, be specially in rice district, the Chinese middle and lower reach of Yangtze River, divide early, middle and late rice to divide the season sowing described rice material, wherein early rice is in sowing in 25 days ± 2 days May in every year, in, late rice is in sowing in 10 days ± 2 days May in every year, make to be in annual July 25 its heading stage between August 15; The 4-5 leaf is transplanted during the phase in the land for growing field crops, 2 sub-districts of every part of material plantation, every sub-district plantation 4 row totally 40 strains; Normal rich water quality management;
2) grouping of measuring material with decide strain: according to test material by actual heading stage mutually approximately principle be divided into a plurality of test group, every group of test material is no more than 10 parts, require the Rice Population growth consistent, and surveyed the chlorophyll content value (SPAD) of each plant stem sword-like leave before the phase with chlorophyll measuring instrument (SPAD-502 type) in the beginning fringe, select in the sub-district the most approaching individual plant of SPAD value to decide strain 6 strains and number, decide the strain individual plant as what carry out photosynthetic rate mensuration;
3) photosynthetic rate is measured: in the week, select the maximum air temperature to be higher than 33 ℃ fine day behind the beginning fringe, 9:00 begins the sword-like leave of selected rice plant is carried out photosynthetic rate mensuration the morning of every day, and measuring the position is 1/3 place, front portion of sword-like leave blade; 1 minute each determination data time spent; Every group of material finished mensuration in 1 hour; 13:00 on the same day begins the repetition mensuration in the morning; Repeating the 2nd time again in the week age of back measures; Adopt the coupling of the portable photosynthetic rate determination instrument composition measuring of the portable photosynthetic rate determination instrument of separate unit or many system, make automatic mensuration leaf temperature, stomatal conductance, intercellular CO
2Concentration, environmental temperature, humidity parameter;
4) interpretation of result and checking: determination data is converted to Office Excel tables of data, get 6 strain measurement result calculating mean values, analyze data stability between repetition, the stomatal conductance that obtains simultaneously during with mensuration, leaf temperature parameter is analyzed the reasonability of photosynthetic value, irrational test value is carried out repetition measurement or rejecting, calculate each test material photosynthetic rate drop-out value;
5) particularity of different materials colony is handled: the primary election of breeding progeny material, can not establish repetition, each individual plant was finished mensuration in the colony in 2 hours, measure 120 strains every day, according to the difference at heading stage, whole colony can finish mensuration in several days, for close more material breeding time, interval sowing is adjusted to and measures different heading stages after the packets available, uses 2 or many mensuration systems to finish the evaluation work of described experimental scale; Or
(2) mensuration of photosynthetic rate letdown point temperature under the artificial illumination and temperature condition:
1) plantation of test material: employing is the breeding time of the method adjusting rice plant of sowing in batches, and the rice material for examination is separated heading stage; The rice plant of participating in the experiment was transplanted to cultivating alms bowl in 5 leaf phases, every material 5 alms bowls of planting, the chlorophyll content SPDA value of sword-like leave is measured in every potting 3 strains with the chlorophyll measuring instrument before the beginning fringe, select the individual plant of SPAD value unanimity to decide strain and carry out photosynthetic mensuration;
2) artificial illumination and temperature condition setting: select large-scale climatic chamber of the formula of stepping into or growth case for use, the detected materials of heading is put into artificial illumination and temperature growth case or climatic chamber for 2 parts, adopt Biological lamp illumination, the setting light intensity is 20000lx, adapting to 2 hours laggard trip temperatures of illumination regulates, make the temperature setting be warming up to 38 ℃ by 28 ℃, the rice leaf surface temperature changes in 30 ℃-42 ℃, and the temperature of the above evenly rose in 3 hours;
3) photosynthetic rate is measured: in the temperature uphill process, photosynthetic mensuration is carried out once with interior in every interval 20 minutes; Each measure 2 parts of rice materials, each testing of materials 3 strain is finished in 5 minutes in limited time, and statistical data is averaged, and to 2 parts of materials mensurations that hockets, the peak that reaches setting to leaf temperature is to finish mensuration more than 42 ℃;
4) data analysis: be converted to Office Excel tables of data and draw leaf table actual temperature and the photosynthetic rate relation curve with the photosynthetic rate of analyzer acquisition and the test data values of blade actual temperature, the photosynthetic rate of finding out every part of test material obviously descends corresponding leaf table temperature value as photosynthetic letdown point temperature; With photosynthetic letdown point temperature is to be the responsive to temperature type material below 35 ℃; 35 ℃-37 ℃ is medium sensitivity section bar material; Be that the high-temperature stable material is as evaluation criterion more than 37 ℃.
2, method according to claim 1, wherein the described assay method of step (1) also is included in the fine high-temperature stability index of estimating photosynthetic capacity of paddy rice with the difference of the rice leaf Net Photosynthetic Rate that 9:30 and 13:30 measured of high temperature.
3, method according to claim 1, wherein the described assay method of step (2) also comprise utilize temperature that artificial illumination and temperature growth case or artificial illumination and temperature climatic chamber be provided with in limited time between in 3 hours by the parameter setting of 28 ℃ of even variation to 38 ℃, the intensity of illumination parameter is provided with, and estimates the standard of paddy rice high-temperature stability power with photosynthetic rate letdown point temperature.
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| CN105631758A (en) * | 2016-01-11 | 2016-06-01 | 长江大学 | Method for identifying paddy rice kind high temperature resistance performance |
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| CN108399650A (en) * | 2018-01-26 | 2018-08-14 | 中国农业大学 | A kind of chlorophyll content prediction technique |
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| CN104303867A (en) * | 2014-10-16 | 2015-01-28 | 中国科学院遗传与发育生物学研究所 | Method for comparing hard-light resistance of plants |
| CN105638261A (en) * | 2015-12-29 | 2016-06-08 | 四川省农业科学院作物研究所 | Rice high-yield population lodging resistance identification method |
| CN105638261B (en) * | 2015-12-29 | 2018-08-31 | 四川省农业科学院作物研究所 | A kind of Rice Population with High Yield lodging resistance identification method |
| CN105631758A (en) * | 2016-01-11 | 2016-06-01 | 长江大学 | Method for identifying paddy rice kind high temperature resistance performance |
| CN105631758B (en) * | 2016-01-11 | 2019-03-26 | 长江大学 | A kind of rice varieties high temperature resistance identification method |
| CN108399650A (en) * | 2018-01-26 | 2018-08-14 | 中国农业大学 | A kind of chlorophyll content prediction technique |
| CN108399650B (en) * | 2018-01-26 | 2020-06-30 | 中国农业大学 | Chlorophyll content prediction method |
| CN108770614A (en) * | 2018-07-04 | 2018-11-09 | 山东省农业科学院作物研究所 | A kind of Heat Tolerance of Wheat Cultivars evaluation method |
| CN111466265A (en) * | 2020-05-25 | 2020-07-31 | 华东师范大学 | Method for improving photosynthetic electron transfer under high-temperature stress of corn |
| CN111466265B (en) * | 2020-05-25 | 2021-05-07 | 华东师范大学 | Method for improving photosynthetic electron transfer under high-temperature stress of corn |
| CN114002194A (en) * | 2021-10-22 | 2022-02-01 | 安徽袁粮水稻产业有限公司 | Method for identifying photosynthetic capacity of improved rice plant |
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