CN105265206B - A kind of assay method of saline land greening nursery stock salt resistance ability - Google Patents
A kind of assay method of saline land greening nursery stock salt resistance ability Download PDFInfo
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- CN105265206B CN105265206B CN201510784701.3A CN201510784701A CN105265206B CN 105265206 B CN105265206 B CN 105265206B CN 201510784701 A CN201510784701 A CN 201510784701A CN 105265206 B CN105265206 B CN 105265206B
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- soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/041—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a solid body
Abstract
The invention discloses " a kind of assay method of saline land greening nursery stock salt resistance ability ", belong to field of garden technology.This method chooses the annual seedling of growth uniformity, after promoting rooting treatment, is planted in seedling planting bag, after after Survival rate of nursery stock in field planting bag, transplants in the salt-soda soil of soil salt content graded；Determine to weigh the nursery stock individual plant of nursery stock salt resistance ability, the root region soil salt content of the selected nursery stock individual plant of investigation, with the salt resistance ability of this determination nursery stock with salt damage situation, selection according to surviving for nursery stock is increased with soil salt content；The highest soil salt content that the minimum of the nursery stock salt resistance ability determined using Various Seasonal is suitably transplanted as nursery stock in the salt-soda soil；Greening seedling is selected and is configured finally according to the actual soil salt content in salt-soda soil.
Description
Technical field
The present invention relates to " a kind of assay method of saline land greening nursery stock salt resistance ability ", belong to field of garden technology.
Background technology
Salt-soda soil transplanted seedling tree, due to being influenceed to be difficult to survive by soil salt, selects suitable salt tolerant nursery stock to be transplanted
It is the element task of salt-soda soil nursery stock production and greening, and rational selection of seedling method is the premise that nursery stock is correctly selected.Mesh
Before, in saline land greening practice, the most of actual plant experience for deriving from salt-soda soil of selection of nursery stock species lacks to nursery stock
In the objective evaluation of given area suitable planting soil salt content, it is impossible to seeds are carried out according to the actual salinity situation in salt-soda soil
Reasonable disposition, plant percent is low；And existing nursery stock Tolerant salt method is mainly in controllable environment or short term tests result
On the basis of identification is compared to the salt tolerance of nursery stock, the nursery stock identified application effect in saline land greening is poor, and
It is cumbersome.The invention provides a kind of method for determining nursery stock salt resistance ability on the spot in salt-soda soil, with easy to operate, application
By force, high-throughout feature.
The content of the invention
Technical problem
Present invention aims at provide a kind of easy to operate, application strong nursery stock salt resistance ability assay method.
Technical scheme
(1) plot is selected：In salt-soda soil on the spot, plot (different plot of the selection soil salt content luffing 0 ‰ -10 ‰
Or same plot)；Then the complete investigation soil salt content distribution situation in these plot, it is respectively 0- to choose soil salt content
2 ‰, 2-4 ‰, 4-6 ‰, 6-8 ‰, 8-10 ‰ trial zone, trial zone are flattened, weeding, and surrounding has dug gutter, it is ensured that row
Current are smooth.
Soil salt content researching determining method is：In pre-selection plot, measuring point is selected at interval of 50cm with checkerboard type, first
The soil conductivity value of each point 5cm, 15cm, 25cm soil layer is determined with soil conductivity testing instrument, then according to soil conductivity value
To the standard curve of soil salt content, the soil salt content of each measuring point different soil is calculated, each measuring point three is finally calculated
The average value of individual soil layer soil salt content as the measuring point soil salt content.
The standard curve making method is：First 15cm is determined at random on the spot with portable soil conductivity meter in salt-soda soil
The soil conductivity of soil layer, chooses the measuring point that 10 soil conductivities change in gradient, and soil conductivity spacing gradient value is
0.8mS/cm-1.2mS/cm, and the soil conductivity of each measuring point 5cm, 15cm, 25cm soil layer is recorded, then adopted with soil sampler
Collect the pedotheque of each measuring point 0-30cm soil layers, take back laboratory, with mass method (Lu Rukun, soil agrochemistry analysis side
Method, Chinese agriculture Science Press, 1999) its soil salt content is determined, finally with each measuring point 5cm, 15cm, 25cm tri-
The soil salt content of the average value of soil layer soil conductivity and its 0-30cm soil layer, makes field and surveys soil conductivity to soil
The standard curve of salt content.Used conductivity meter is that the U.S. produces portable soil conductivity meter, model：
Spectrum2265FS。
(2) selection of seedling and field planting plastic bag cultivation are planted：Choose annual and growth uniformity strong from normal soil nursery
Kang Shumiao is used as experiment nursery stock；Before plant, selected nursery stock is first subjected to rush rooting treatment, then with the normal arable land loose fertilizer in top layer
Fertile soil earth in field planting bag, periodically waters transplanted seedling tree, waits Survival rate of nursery stock.
The growth-promoting root processing method is：It is small that root 1 is soaked in nursery stock root to be tested with ABT3 root-inducing powders 20ppm solution
When.
(3) salt-soda soil is transplanted：Wait after the Survival rate of nursery stock being planted in field planting bag, nursery stock is moved together with field planting bag soil
Plant in the experimental plot of salt-soda soil, every kind of nursery stock to be tested plants 40 plants in the experimental plot of each salinity level.
(4) determination of nursery stock salt resistance ability individual plant is weighed：Various Seasonal is survived and salt damage to nursery stock after nursery stock transplanting
Condition survey, it is determined that weighing the nursery stock individual plant of nursery stock salt resistance ability.
Weigh nursery stock salt resistance ability individual plant selection of seedling method be：
I, reaches the cell of certain level when soil salt, and plant percent is higher than 75%, and adjacent higher salinity cell
When plant percent is less than 25%, most heavy 20 plants of salt damage degree is selected to survive nursery stock in the salinity cell single as candidate nursery stock
Strain；
II, reaches the cell of certain level when soil salt, plant percent less than 75%, higher than 50%, and it is adjacent compared with
High salinity cell plant percent is less than 25%, and most light 20 plants of the nursery stock individual plant of surviving of salt damage degree is selected as waiting in the cell
Select nursery stock individual plant；
III. when soil salt reaches the cell of certain level, plant percent less than 50%, higher than 25%, and it is adjacent compared with
When high salinity cell plant percent is less than 25%, most light 10 plants of salt damage degree is selected to survive nursery stock in the cell, simultaneously
Salt damage degree most heavy 10 plants of nursery stock individual plant is selected in the adjacent cell compared with low salt, collectively as candidate's nursery stock individual plant.
(5) determination of nursery stock salt resistance ability：
A measuring point totally 3 points are respectively taken at interval of 120 degree at selected nursery stock individual plant root offset trunk 5cm, are used
Portable soil conductivity meter determines the soil conductivity of each point 5cm, 15cm, 25cm soil layer, then according to soil conductivity
Soil conductivity is converted into soil salt content with the standard curve of soil salt content.
After the soil salt content average value for calculating each three soil layers of measuring point, then calculate the soil of 3 measuring points
Salt content average value, and it is used as using this value the root region soil salt content of nursery stock individual plant.
Then average value, standard deviation and the variation lines of the root region soil salt content of 20 selected nursery stock individual plants are calculated
Number (coefficient of variation=standard deviation/average value × 100%)；Go out the salt resistance ability of nursery stock, computational methods according to above numerical computations
For：The root region soil salt content average value of selected 20 nursery stock individual plants × (the 1- coefficient of variation).
The minimum of the last nursery stock salt resistance ability using Various Seasonal researching determining is as nursery stock in salt-soda soil suitable planting
Highest soil salt content, be the salt resistance ability of measured saline land greening nursery stock.The Various Seasonal refer to summer,
Autumn-winter and spring next year.
Beneficial effect
The inventive method is repeatedly determined to nursery stock salt resistance ability in Various Seasonal on the spot in salt-soda soil, and demonstrating this method can
The salt resistance ability of nursery stock is rationally weighed, and measurement result can be directly used for instructing the configuration of saline land greening nursery stock.
Present invention determine that highest soil salt content of the nursery stock in the salt-soda soil suitable planting, the measurement result degree of accuracy is high, it is real
Border application is strong；The greening seedling suitably transplanted, selected seedling are directly selected during greening according to alkaline land soil salinity actual content
Wooden survival rate is high, and afforestation effect is good.Meanwhile, the present invention using portable soil conductivity meter due to quickly determining nursery stock root area soil
Earth salt content, realizes and nursery stock high flux, simple and quick salt resistance ability is determined.
Embodiment
The present invention is further illustrated below by the embodiment of saline land greening nursery stock salt resistance ability assay method.
1st, plot selection and preparation
In Nantong City Coastal beach salt-soda soil, soil salt content has been preselected first in 0 ‰~10 ‰ scopes not
Same plot；Then the complete investigation soil salt content distribution situation in these plot, choose soil salt content be respectively 0-2 ‰,
2-4 ‰, 4-6 ‰, 6-8 ‰, 8-10 ‰ five pieces 3 meters wide, 15 meters of long trial zones, then leveling, weeding, surrounding have dug draining
Ditch, it is ensured that trial zone draining is smooth, standby.
Soil salt content researching determining method is：In pre-selection plot, measuring point is selected at interval of 50cm with checkerboard type, first
The conductivity value of each point 5cm, 15cm, 25cm soil layer is determined with soil conductivity testing instrument, then according to soil conductivity value to soil
The standard curve of earth salt content, calculates the soil salt content of each measuring point different soil, finally calculates each three soil of measuring point
Layer soil salt content average value as the measuring point soil salt content.
The standard curve making method is：First 15cm is determined at random on the spot with portable soil conductivity meter in salt-soda soil
The soil conductivity of soil layer, chooses the measuring point that 10 soil conductivities change in gradient, and soil conductivity spacing gradient value is
0.8mS/cm-1.2mS/cm, and the soil conductivity of each measuring point 5cm, 15cm, 25cm soil layer is recorded, then adopted with soil sampler
Collect the pedotheque of each measuring point 0-30cm soil layers, take back laboratory, with mass method (Lu Rukun, soil agrochemistry analysis side
Method, Chinese agriculture Science Press, 1999) its soil salt content is determined, finally with each measuring point 5cm, 15cm, 25cm tri-
The soil salt content of the average value of soil layer soil conductivity and its 0-30cm soil layer, makes field and surveys soil conductivity value to soil
The standard curve of earth salt content.Used conductivity meter is that the U.S. produces portable soil conductivity meter, model：
Spectrum2265FS。
2nd, the selection of nursery stock
Little leaf boxwood, Euonymus japonicus, photinia glabra, Ligustrum quihoui, Ligustrum vicaryi 5 are have chosen from normal soil nursery
Annual and growth uniformity the healthy sapling of seeds is used as experiment nursery stock.
3rd, seedling planting
Before nursery stock transplanting, root is first soaked into 1 hour in selected nursery stock root with ABT3 root-inducing powders 20ppm solution, Ran Houyong
The normal arable land loose rich soil in top layer in field planting bag, periodically waters transplanted seedling tree, waits Survival rate of nursery stock.
4th, salt-soda soil is transplanted
After the sapling being planted in field planting bag survives, it is transplanted on April 1st, 2014 in ready salt-soda soil,
Every kind of 40 plants of seedling growth in the trial zone of each salinity level, first presses line-spacing in each experimental plot in salt-soda soil before transplanting
50cm, spacing in the rows 30cm dig tree well, then plant sapling in tree well together with soil in field planting bag.
5th, Survival rate of nursery stock condition survey and the determination of measurement nursery stock salt resistance ability individual plant
Investigation survives situation with the increase sapling of soil salt content.From table 1, in the range of the soil salts of 0-2 ‰
Selected 5 seeds survival rates are reduced more than 95% with the increase survival rate of soil salt content, after soil salt content is more than 4 ‰,
Each seeds survival rate starts to be decreased obviously, after soil salt content reaches 6 ‰, and 5 seeds survival rates are less than 50%.
Each seeds survives strain number under the conditions of the different salinities of table 1.
According to Survival rate of nursery stock and salt damage situation, it is determined that weighing the individual plant nursery stock of nursery stock salt resistance ability.Specific method is：
I, reaches the cell of certain level when soil salt, and plant percent is higher than 75%, and adjacent higher salinity cell
When plant percent is less than 25%, most heavy 20 plants of salt damage degree is selected to survive nursery stock in the salinity cell single as candidate nursery stock
Strain；
II, reaches the cell of certain level when soil salt, plant percent less than 75%, higher than 50%, and it is adjacent compared with
High salinity cell plant percent is less than 25%, and most light 20 plants of the nursery stock individual plant of surviving of salt damage degree is selected as waiting in the cell
Select nursery stock individual plant；
III. when soil salt reaches the cell of certain level, plant percent less than 50%, higher than 25%, and it is adjacent compared with
When high salinity cell plant percent is less than 25%, most light 10 plants of salt damage degree is selected to survive nursery stock in the cell, simultaneously
Salt damage degree most heavy 10 plants of nursery stock individual plant is selected in the adjacent cell compared with low salt, collectively as candidate's nursery stock individual plant.
6th, the determination of nursery stock salt resistance ability
A point totally 3 measuring points are respectively taken at interval of 120 degree at selected nursery stock individual plant root offset trunk 5cm, are used
Portable soil conductivity meter determines the soil conductivity of each point 5cm, 15cm, 25cm soil layer, then according to soil conductivity
Soil conductivity is converted into soil salt content with the standard curve of soil salt content.
After the soil salt content average value for calculating each three soil layers of measuring point, then calculate the soil of 3 measuring points
Salt content average value, and it is used as using this value the root region soil salt content of selected nursery stock individual plant.
Then average value, standard deviation and the variation lines of the root region soil salt content of 20 selected nursery stock individual plants are calculated
Number (coefficient of variation=standard deviation/average value × 100%)；Go out nursery stock salt resistance ability according to above numerical computations, computational methods are：
The root region soil salt content average value of selected 20 nursery stock individual plants × (the 1- coefficient of variation).
The variation of each seeds candidate nursery stock individual plant rhizosphere soil salt content of table 2.
To avoid the influence that climate change is determined to nursery stock salt resistance ability, respectively at June 20 (summer), November 5 days
The salt resistance ability of nursery stock is investigated and determined to April 20 (spring) three of (autumn-winter) and next year crucial seasons, finally with
The minimum of the nursery stock salt resistance ability of three researching determinings as nursery stock the salt-soda soil suitable planting highest soil salt content,
The salt resistance ability of as measured saline land greening nursery stock.
The highest soil salt content (‰) of each seeds Various Seasonal salt resistance ability of table 3. and suitable planting
Salt damage symptom of the soil salt content of table 4. close to each seeds during nursery stock salt resistance ability
The salt damage symptom (table 4) when root region soil salt content is close to nursery stock salt resistance ability is observed, is found and the salt of 0-2 ‰
The nursery stock grown under point level is substantially slowed down compared to growth rate, and nursery stock shows obvious salt damage symptom, but nursery stock remains to just
Often survive, substantially meet the demand of greening, this, which demonstrates this method, can rationally weigh the salt resistance ability of nursery stock, and measurement result can
It is directly used in the configuration for instructing saline land greening nursery stock.
Claims (7)
1. a kind of assay method of saline land greening nursery stock salt resistance ability, it is characterised in that：
1) plot is selected：Plot of the soil salt content luffing 0 ‰ -10 ‰ is selected in salt-soda soil；Then selected in these plot
It is respectively 0-2 ‰, 2-4 ‰, 4-6 ‰, 6-8 ‰, 8-10 ‰ trial zone to take soil salt content, and leveling, weeding, surrounding ensures row
Current are smooth；
2) selection of seedling and field planting plastic bag cultivation are planted：Annual and growth uniformity healthy sapling is chosen from nursery as experiment
Nursery stock；Before plant, selected nursery stock is first subjected to rush rooting treatment, then nursery stock is planted with the normal arable land loose rich soil in top layer
Plant in field planting bag, periodically water, wait Survival rate of nursery stock；
3) salt-soda soil is transplanted：Wait after the Survival rate of nursery stock being planted in field planting bag, nursery stock is transplanted together with soil in field planting bag
In the experimental plot for entering the different salinity levels in salt-soda soil, it is ensured that nursery stock to be tested has in the experimental plot of each salinity level
40 plants；
4) determination of nursery stock salt resistance ability：Nursery stock transplanting, to Survival rate of nursery stock and salt damage condition survey, selectes weighing apparatus after Various Seasonal
Measure 20 nursery stock individual plants of nursery stock salt resistance ability；And the soil salt content in selected 20 nursery stock individual plant root areas is determined, then calculate
Go out the nursery stock salt resistance ability of Various Seasonal measure；
5) minimum of the last nursery stock salt resistance ability determined using Various Seasonal as nursery stock salt-soda soil suitable planting highest
Soil salt content, is the salt resistance ability of measured saline land greening nursery stock.
2. according to the method described in claim 1, it is characterised in that the 20 nursery stock individual plants choosing for weighing nursery stock salt resistance ability
Selection method is：
I, reaches the cell of certain level when soil salt, and plant percent is higher than 75%, and adjacent higher salinity cell nursery stock
When survival rate is less than 25%, in most heavy 20 plants of salinity cell selection salt damage degree of the plant percent higher than 75% into live seedling
Carpentery workshop is candidate nursery stock individual plant；
II, reaches the cell of certain level when soil salt, and plant percent is less than 75%, higher than 50%, and adjacent compared with high salt
Cell plant percent is divided to be less than 25%, in cell selection salt damage degree of the plant percent less than 75%, higher than 50% most
Light survives 20 plants of nursery stock individual plant as candidate's nursery stock individual plant；
III. when soil salt reaches the cell of certain level, plant percent is less than 50%, higher than 25%, and adjacent compared with high salt
When dividing cell plant percent less than 25%, salt damage journey is selected in the plant percent is less than the 50%, cell higher than 25%
Most light 10 plants of degree survives nursery stock, while salt damage degree most heavy 10 plants of nursery stock individual plant is selected in the adjacent cell compared with low salt,
Collectively as candidate's nursery stock individual plant.
3. method according to claim 1 or 2, it is characterised in that the survey of described nursery stock individual plant root region soil salt content
Determine and computational methods are：Around selected nursery stock individual plant root offset trunk 5cm a measuring point totally 3 is taken at interval of 120 degree
It is individual, the soil conductivity of each point 5cm, 15cm, 25cm soil layer is determined with portable soil conductivity meter, then according to standard
Soil conductivity is converted into soil salt content by curve；Calculate the soil salt content average value of each three soil layers of measuring point
Afterwards, then the soil salt content average value of 3 measuring points is calculated, and is used as using this value the root region soil saliferous of selected nursery stock individual plant
Amount, used conductivity meter is that the U.S. produces portable soil conductivity meter, model：Spectrum 2265FS；Selected measurement seedling
Average value × (the 1- coefficient of variation) of 20 nursery stock individual plant root region soil salt content of wooden salt resistance ability as nursery stock salt tolerant energy
Power.
4. method according to claim 3, it is characterised in that the preparation method of the standard curve is：First with soil electricity
Electrical conductivity instrument determines soil conductivity in salt-soda soil at random on the spot, chooses 10 soil conductivities differences and soil conductivity gradient becomes
The measuring point of change, soil conductivity spacing gradient value is 0.8mS/cm-1.2mS/cm, records each measuring point 5cm, 15cm, 25cm
The soil conductivity of soil layer, then gathers the pedotheque of each measuring point 0-30cm soil layers, takes back laboratory, determined with mass method
Soil salt content, finally with the average value and its 0-30cm of tri- soil layer soil conductivities of each measuring point 5cm, 15cm, 25cm
The soil salt content of soil layer, makes standard curve of the field actual measurement soil conductivity to soil salt content.
5. method according to claim 1 or 2, it is characterised in that the Various Seasonal refers to summer, autumn-winter and secondary
Spring in year.
6. method according to claim 3, it is characterised in that the Various Seasonal refers to summer, autumn-winter and spring next year
Season.
7. method according to claim 4, it is characterised in that the Various Seasonal refers to summer, autumn-winter and spring next year
Season.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105850632A (en) * | 2016-04-06 | 2016-08-17 | 天津泰达绿化集团有限公司 | Method for cultivating cornus officinalis in saline-alkali area |
| CN105850420A (en) * | 2016-04-06 | 2016-08-17 | 天津泰达绿化集团有限公司 | Method for cultivating cercis gigantean in saline-alkali area |
| CN105850631A (en) * | 2016-04-06 | 2016-08-17 | 天津泰达绿化集团有限公司 | Method for cultivating drooping gingkoes in saline-alkali area |
| CN105850630A (en) * | 2016-04-06 | 2016-08-17 | 天津泰达绿化集团有限公司 | Transplanting method of Picea pungens in coastal saline area |
| CN105900763A (en) * | 2016-04-13 | 2016-08-31 | 天津泰达绿化集团有限公司 | Northeast Chinese yew transplanting method in coast saline-alkaline region |
| CN112051359A (en) * | 2020-07-27 | 2020-12-08 | 滨州职业学院 | Method for identifying saline-alkali resistance of wheat according to field growth tracing |
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| CN1018701B (en) * | 1989-10-24 | 1992-10-21 | 段世杰 | Plant cultivated method of saline-alkali beach |
| CN100435617C (en) * | 2005-04-05 | 2008-11-26 | 刘延春 | Chinese wildrye sprouts vessel cultivation method |
| CN101843197B (en) * | 2010-04-06 | 2012-05-30 | 上海市园林科学研究所 | Selection method used for improving seashore salt-affected soil plants |
| CN102511325B (en) * | 2011-12-26 | 2013-10-16 | 杭州市园林绿化股份有限公司 | Method for sieving salt-tolerant varieties of osmanthus fragrans |
| RU2544381C1 (en) * | 2013-09-09 | 2015-03-20 | Эльвира Батыревна Дедова | Method of rice cultivation on saline lands |
| CN104206217B (en) * | 2014-08-07 | 2017-02-01 | 江苏沿海地区农业科学研究所 | Rice-variety salt-tolerance evaluating method |
| CN104521474A (en) * | 2014-12-04 | 2015-04-22 | 中国农业科学院棉花研究所 | Cotton variety salt tolerance screening method |
| CN104604554B (en) * | 2015-01-13 | 2017-02-01 | 江苏省农业科学院 | Salt-tolerant sorgo germplasm identification method |
| CN104730113A (en) * | 2015-01-22 | 2015-06-24 | 中国科学院东北地理与农业生态研究所 | Method for realizing online measurement of electrical conductivity by utilizing saline-alkali soil crack length |
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