CN106526083B - A kind of method of Heat Tolerance of Wheat Cultivars identification - Google Patents
A kind of method of Heat Tolerance of Wheat Cultivars identification Download PDFInfo
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Abstract
The present invention provides a kind of method of Heat Tolerance of Wheat Cultivars identification.Method and step provided by the invention includes that the judgement that heats the heat resistant variety that canopy Heat tolerance identification, Heat tolerance identification index record and heat susceptibility index calculate, kind heat resistance judges, stablize is built in intelligent greenhouse Heat tolerance identification, crop field.Heat tolerance identification method provided by the invention, qualification result are accurate and reliable.The present invention is built with crop field by intelligent greenhouse heat tolerance identification and heats canopy heat tolerance identification and be combined, year border heat tolerance identification results contrast is combined with different ecological region heat tolerance identification results contrast, Heat tolerance identification and screening to Wheat Germplasm Resources and breeding parent ensure that China's grain security is of great significance suitable for the heat-resisting wheat breed of Huang-Huai-Hai area of wheat plantation.The identification of indicator that the present invention chooses is easy to measure, and by indoor identification and crop field identification mutually confirmation, can really reflect the heat resistance of wheat breed.
Description
Technical field
The testing indexs such as heat susceptibility index are the present invention relates to the use of, the physiological and biochemical procedure of Heat Tolerance of Wheat Cultivars is studied, especially relates to
A kind of and method of Heat Tolerance of Wheat Cultivars identification.
Background technology
Wheat is the important cereal crops in China, and belong to the cool habit of happiness especially has with yield important within the season of growth
The pustulation period of relationship, it is easy to be influenced by heat stress caused by abnormal high temperature weather, yield is caused to decline, quality reduces.
Often there is hot dry wind in the wheat belts such as the Yellow River and Huai He River sea region and Xinjiang, Wheat in Grain Filling Stage, and temperature is sometimes up to 32 DEG C or more, causes
Wheat yield 10-20%.Northern China and middle and lower reach of Yangtze River Winter Wheat Area are also it occur frequently that high temperature damage, causes wheat yield.
Identification, screening wheat heat resistant variety and germ plasm resource, it is to solve Heat Tolerance of Wheat Cultivars to carry out Heat Tolerance of Wheat Cultivars breeding
Fundamental way.Plantation adapts to the wheat breed of high temperature stress, is the important channel for realizing and keeping Yield Potential of Wheat.
Existing heat-resisting Journal of Sex Research lays particular emphasis on more and builds plastic greenhouse progress heat-resistance test by crop field, but single crop field is resistance to
Heat research is often influenced by factors such as weather, environment, wheat growth periods, and heat resistance result of study is often unstable
Fixed, the heat resistant variety year of identification, difference was larger between different zones between border, cannot accurately reflect the heat resistance of kind.The present invention will
The Heat tolerance identification of controllable temperature heats the Heat tolerance identification in canopy with crop field and is combined in intelligent greenhouse, by intelligent greenhouse and
The mutual confirmation of crop field kind Heat tolerance identification result, further improves the accuracy of wheat breed Heat tolerance identification, this is resistance to
Hot identification method, there is not been reported both at home and abroad at present.
Invention content
To solve the above technical deficiency, the present invention provides a kind of methods of Heat Tolerance of Wheat Cultivars identification.
The present invention is achieved by the following technical solutions：
A kind of method of Heat Tolerance of Wheat Cultivars identification provided by the invention, includes the following steps：
（1）Heat tolerance identification in intelligent greenhouse：Wheat seed low temperature vernalization treatment 40 days is sowed in intelligent greenhouse,
Diurnal temperature is set as 20/16 DEG C and duration is set as planting under the conditions of 16/8h round the clock, and florescence mark stem is bloomed, and fringe simultaneously hang by the same day
Board spends rear diurnal temperature to be adjusted to 25/16 DEG C；10 days after spending, it is divided into high-temperature process group and control group；High-temperature process group, daytime
High-temperature process temperature is set as 33 to 39 DEG C, and night temperature is set as 20 DEG C；Control group, diurnal temperature are set as 25/16 DEG C；At each temperature
The material of reason is planted respectively in the different planter units of intelligent greenhouse, independent temperature control, until Growth of Wheat to maturation；
（2）Crop field, which is built, heats Heat tolerance identification in canopy：Crop field Heat tolerance identification experiment, takes two factor split block designs,
The heat-resisting Stress treatment in primary area and control two are handled, kind random alignment in secondary area, 3 repetitions；
High temperature stress processing：It 20 days after wheat flower, that is, is in the milk the later stage, under fine day or cloudy weather, in what is built
It heats and carries out high temperature stress in canopy, daily high temperature stress 5 hours, i.e., 11:00-16:00；
Control treatment is cultivated by normal condition；
（3）Heat tolerance identification index records and heat susceptibility index calculates：Flowering stage of wheat, maturity period, boot leaf death phase are recorded,
Measure mass of 1000 kernel；Calculate Grain-filling period and heat susceptibility index S；
S=(1−YD/YP)/(1−/)；
Wherein, YD is mass of 1000 kernel or Grain-filling period of certain wheat breed under high-temperature process or heat stress, and YP is certain product
Mass of 1000 kernel in normal circumstances or Grain-filling period are planted,Be all kinds high-temperature process or heat stress processing under thousand
The average value of weight or Grain-filling period,For all kinds average value of mass of 1000 kernel or Grain-filling period in normal circumstances；
According to heat susceptibility index formula, mass of 1000 kernel heat susceptibility index and Grain-filling period heat susceptibility index are calculated；
（4）Heat resistance judges：Mass of 1000 kernel heat susceptibility index S ＜ 1 are then heat resistance kind；Mass of 1000 kernel heat susceptibility index S >=1, then
For thermo-responsive kind；
In the heat resistance kind of mass of 1000 kernel heat susceptibility index S ＜ 1, Grain-filling period heat susceptibility index S ＜'s 1, then kind is anti-
It is hot stronger；
（5）The judgement of stable heat resistance kind：Intelligent greenhouse Heat tolerance identification is heat resistance kind, and different annual
It is heat resistance kind to heat Heat tolerance identification in canopy with the crop field in different ecological region, then is stable heat resistance kind.
A kind of method of Heat Tolerance of Wheat Cultivars identification provided by the invention：The step（1）Heat tolerance identification in intelligent greenhouse,
Heat treatment preferable temperature be set as 33 DEG C, 35 DEG C, 37 DEG C, 39 DEG C or in which combination.
A kind of method of Heat Tolerance of Wheat Cultivars identification provided by the invention：The step（1）Heat tolerance identification in intelligent greenhouse,
It is 35 DEG C to be heat-treated preferred temperature.
A kind of method of Heat Tolerance of Wheat Cultivars identification provided by the invention：The step（2）Crop field heats heat resistance in canopy and reflects
There is fixed preferably choose representative 2 of planting area or more testing site to be identified.
A kind of method of Heat Tolerance of Wheat Cultivars identification provided by the invention：The step（2）Crop field heats heat resistance in canopy and reflects
It is fixed identify within preferably 2 years or more.
A kind of method of Heat Tolerance of Wheat Cultivars identification provided by the invention：It is resistance to that this identification is preferably applied to the Yellow River and Huai He River sea region wheat
Hot identification.
A kind of method of Heat Tolerance of Wheat Cultivars identification provided by the invention：Identify place preferably with year in Jinan, Shandong Province, peace
Emblem Hefei, Zhengzhou, henan and Hebei Shijiazhuang.
The beneficial effects of the invention are as follows：Heat tolerance identification method provided by the invention, qualification result are accurate and reliable.The present invention
By being identified in intelligent greenhouse and crop field builds and heats in canopy identification and be combined, year border heat tolerance identification results contrast and different ecological
Region heat tolerance identification results contrast is combined, and reasonably selects heat tolerance identification region, to the resistance to of Wheat Germplasm Resources and breeding parent
Hot identification and screening improve wheat yield suitable for the heat-resisting wheat breed of Huang-Huai-Hai area of wheat plantation, ensure national food peace
It is of great significance entirely.The identification of indicator that the present invention chooses is easy to measure, and passes through indoor identification and crop field identification mutually confirmation, energy
The heat resistance of enough true reflection wheat breeds.
Description of the drawings
Fig. 1 heats canopy.
Specific implementation mode
Further detailed description is done to the present invention with reference to embodiment：
Heat Tolerance of Wheat Cultivars is identified in 1 2013-2014 year intelligent greenhouses of embodiment
It is carried out in the intelligent greenhouse that Crop Inst. of shandong Prov. Agriculture science Academy builds.On November 18th, 2013, to small
Wheat variety carries out low temperature vernalization.After vernalization 40 days, respectively 20 basins of sowing, every basin plant 4 plants to each wheat breed.Set diurnal temperature and
Duration is set as 20/16 DEG C, grows under 16/8h round the clock.Florescence mark stem is bloomed, and simultaneously the same day is listed for fringe.Meanwhile the life of Post flowering
Long temperature and round the clock duration are adjusted to 25/16 DEG C, 16/8h.10 days after spending, each kind take 10 cone materials move to growth temperature and
Duration is set as 33/20 DEG C, carries out high-temperature process in the greenhouse unit of 16/8h round the clock, until reaching maturity.
Under the conditions of test result shows high-temperature process, main Ear weight, single-strain grain weight, mass of 1000 kernel and Grain-filling period with not
Significant difference between processing（Table 1）.The wheat product of mass of 1000 kernel and Grain-filling period heat susceptibility index S ＜ 1 it can be seen from heat susceptibility index
Kind（System）Respectively Jimai 22, Jimai 23, Lankao 906-4 and LS6109（Table 2）.
1 2013-2014 year intelligent greenhouse wheat Themotalerance difference analysis of table
| Character | Normal processing mean value | It is heat-treated mean value |
| Main grain number per spike | 45.5a | 45.9a |
| Main Ear weight | 1.96a | 1.8b |
| Single-strain grain weight | 3.96a | 3.0b |
| Single-strain grain number | 90.8a | 85.3a |
| Mass of 1000 kernel | 43.5a | 35.3b |
| Grain-filling period | 39.95a | 30.7b |
2 2013-2014 year intelligent greenhouse wheat breed Themotalerances of table measure and its heat susceptibility index
The 2 year crop fields 2013-2014 of embodiment heat Heat Tolerance of Wheat Cultivars in canopy and identify
It tests and is carried out in Crop Inst. of shandong Prov. Agriculture science Academy experimental plot in 2013-2014 years.Each kind
Cell plantation is carried out, 6 rows are planted per area, row is 2 meters long.Take two factor split block designs, different cultivars completely random row in district's groups
Row.10 days after spending, is built in high-temperature process growing area and heat canopy（Fig. 1）, carry out resistance to heat treatment.It can be seen from heat susceptibility index
The wheat breed of mass of 1000 kernel and Grain-filling period heat susceptibility index S ＜ 1（System）Respectively Jimai 35, Jimai 20, Jimai 27 and Shandong are former
502（Table 3）.
The 3 year crop fields 2013-2014 of table heat canopy wheat breed Themotalerance measurement and its heat susceptibility index
3 2014-2015 year intelligent greenhouse Heat tolerance identifications of embodiment
It is carried out in Crop Inst. of shandong Prov. Agriculture science Academy's intelligent greenhouse.First to test material in vernalization room into
Row low temperature vernalization, then carries out potted plant growth.Diurnal temperature before blooming is set as 20/16 DEG C；The diurnal temperature of Post flowering is set
35/20 DEG C, 37/20 DEG C and 39/20 DEG C three heat stress temperature are set to, high temperature stress is handled to wheat seed maturation.
As can be seen from Table 4, main Ear weight, single-strain grain weight, mass of 1000 kernel and the Grain-filling period for the treatment of of different temperature are right
According between different disposal, there are pole significant differences.The basic trend of three high-temperature process results is that temperature is higher, and substance accumulation is got over
Few, main Ear weight, single-strain grain weight, mass of 1000 kernel are lower, and Grain filling duration is shorter.Mass of 1000 kernel under 37 DEG C of processing is handled than 35 DEG C
Under it is slightly higher, single-strain grain weight is slightly higher, but be not up to significant difference.When illustrating that temperature reaches 35 DEG C, wheat is generated
Very serious high temperature stress.When carrying out high temperature resistant research in intelligent greenhouse, completely may be used using 35 DEG C as high temperature stress temperature
To reach heat-resisting research purpose.
In this research, 39 DEG C of Temperature Treatment and contrast difference is maximum, therefore when progress heat susceptibility index calculating, with 39 DEG C
Each index is that stress index is calculated.By calculating the heat-resistance index of 4 indexs, wherein 3 kinds such as Jimai 20, Jimai 22
It is respectively less than 1 with the heat susceptibility index of all indexs of Jimai 23, illustrates that this 3 kinds have preferable heat resistance, and Jimai 20
Qualification result is consistent in intelligent greenhouse heats canopy with crop field with Jimai 22, to further prove our heat tolerance identification results
Reliability.All heat susceptibility indexes of 4 kinds such as tobacco grower 19, Jimai 27, Jimai 35 and LS6109 are all higher than 1, illustrate theirs
Heat resistance is poor, and all indexs of wherein tobacco grower 19 heat in crop field is all higher than 1 in canopy.The heat-resistance index of other 6 kinds, no
It is had differences between index, illustrates that anti-perception falls between（Table 5）.
4 2014-2015 year intelligent greenhouse Themotalerance difference analysis of table
The measurement of each Themotalerance in 5 2014-2015 year intelligent greenhouses of table
The 4 year crop fields 2014-2015 of embodiment heat the experiment of canopy heat tolerance identification
Crop field Heat tolerance identification experiment is carried out in Crop Inst. of shandong Prov. Agriculture science Academy experimental plot, takes two factors
Split block design, different cultivars completely random arrangement in district's groups.In Wheat Plant in Late Filling Period, the canopy progress high temperature side of body is heated by building
Compel.
Crop field heats Grain-filling period, mass of 1000 kernel, the cell production that the heat resistant test in canopy has mainly investigated different disposal
With 4 indexs of unit weight.Wherein the Grain-filling period of Jimai 20 and Jimai 23, mass of 1000 kernel, 4 indexs of cell production and unit weight heat
Sense index is respectively less than 1, shows that the two kind heat resistances are preferable；3 fingers of Grain-filling period, mass of 1000 kernel, cell production of Jimai 2
Target heat susceptibility index is respectively less than 1, shows that the two kind heat resistances are preferable；The grouting of tobacco grower 19, Jimai 06037 and continent member 9369
Duration, mass of 1000 kernel, 4 indexs of cell production and unit weight heat susceptibility index be all higher than 1, show this 3 kinds（System）It is heat-resisting
Property is poor.Remaining 7 kind examines the heat susceptibility index of 4 different indexs, cannot be consistent be more than or less than 1, illustrate these product
The heat resistance of kind falls between（Table 6）.
The 6 year crop fields 2014-2015 of table heat the heat susceptibility index of canopy Themotalerance
The 5 year different ecological regions 2015-2016 crop field of embodiment heats the experiment of canopy heat tolerance identification
Crop field is carried out in 4 planted in different ecological areas such as Jinan, Shandong Province, Zhengzhou, henan, Hefei ,Anhui and Hebei Shijiazhuang to heat
Heat tolerance identification in canopy.Two factor split block designs, the heat-resisting Stress treatment in primary area and control two are taken in crop field Heat tolerance identification experiment
A processing, kind random alignment in secondary area, 3 repetitions.It 20 days after wheat flower, that is, is in the milk the later stage, in fine day or broken sky
Under gas, high temperature stress is carried out in canopy heating.Daily high temperature stress 5 hours, i.e., 11:00-16:00.
1. temperature statistics are analyzed
From heating in terms of canopy internal and external temperature statistical result for 4 experimental points, heat-resisting canopy is built by crop field, 11：00-16：
00 period carries out lid canopy, and can be seen that heat from temperature statistics result highest compared with temperature outside canopy in canopy can be higher by 20.7
DEG C, show, by building gain of heat canopy, to realize the hot environment in canopy（Table 7-10）.
7 Jinan, Shandong Province of table heats canopy internal and external temperature record sheet（℃）
8 Zhengzhou, henan of table heats canopy internal and external temperature record sheet（℃）
9 Hefei ,Anhui canopy internal and external temperature record sheet of table（℃）
10 Hebei Shijiazhuang of table heats canopy internal and external temperature record sheet（℃）
2. Grain-filling period or blade hold green phase analysis
Using portable Chlorophyll meter SPAD-502 plus, the leaf for measuring flowering stage of wheat to maturity period boot leaf is green
Cellulose content, calculates Grain-filling period or blade holds the green phase.
Measurement period is divided into before high temperature stress and latter two period of high temperature stress.Minute before high temperature stress is to open
Flower, Post flowering the 5th day, the 10th day, the 15th day, the 20th day.After high temperature stress, the material in canopy is heated, was measured 1 time every 1 day,
Until wheat is ripe（The material of lid canopy does not compare）.Each kind measures 10 plants every time, removes after measuring peak, is averaging
Value.
It can be seen that from the measurement result of boot leaf SPAD 4 experimental points of value（Table 11-14）, when longer one section after wheat flower
In, boot leaf SPAD values are always maintained at more stable level, but later stage boot leaf SPAD value of being in the milk continuously decreases, and most kinds exist
Boot leaf SPAD values are less than the outer SPAD values of canopy in the canopy of multiple testing sites, and with wheat grain filling process, difference gradually increases, explanation
The heat-resisting canopy that testing site is built, heat stress is produced to wheat, and wheat is caused early ageing phenomenon occur.
Influence of the high temperature stress to boot leaf SPAD values after 11 wheat flower of table（Hefei ,Anhui）
Influence of the high temperature stress to boot leaf SPAD values after 12 wheat flower of table（Zhengzhou, henan）
Influence of the high temperature stress to boot leaf SPAD values after 13 wheat flower of table（Hebei Shijiazhuang）
Influence of the high temperature stress to boot leaf SPAD values after 14 wheat flower of table（Jinan, Shandong Province）
3. changing bulk density is analyzed
Bulk Density of Wheat refers to quality of its seed in unit volume, with gram per liter（g/L）It indicates, size is by including
What many factors such as seed this body structure, moisture, impurity and unsound grain content determined.In general, Grain Ripening is full, ties
The sample that structure is close, particle is small, water content is low, unit weight are larger；Conversely, unit weight is smaller.Therefore, the variation of unit weight can be used as small
A heat-resisting reference index of wheat.The Volume-weight Determinations of three testing sites the result shows that, Heat Treatment on Wheat grain test weight generates not
With the reduction of degree, ranging from 4.6-29.99% or so is reduced（Table 15）.
15 3 testing site Volume-weight Determination results of table
4. mass of 1000 kernel and volume analysis
Mass of 1000 kernel heat susceptibility index and yield heat susceptibility index are compared（Table 16, table 17）, find to utilize mass of 1000 kernel thermal sensation
Index judges the heat resistance of wheat breed, and result is more accurate, and potential output by cell due to being short of seedling disconnected ridge, plot area
The influence of many factors such as measurement, the larger event of error.
16 4 experimental point mass of 1000 kernel of table and heat susceptibility index
17 4 experimental point yield of table and heat susceptibility index
Note：Yield mean value 658.2 in 99 canopy of good star of Henan testing site, the outer yield mean value 649.9 of canopy, heat susceptibility index is-
0.20, the reason is that the outer material of canopy lodges.
Comprehensive analysis above example, it can be seen that gain of heat canopy heat tolerance identification is built in intelligent greenhouse heat tolerance identification and crop field,
Qualification result has differences, and there is also differences between the annual result of crop field heat tolerance identification；Intelligent greenhouse Heat tolerance identification is anti-
Hot kind, and the crop field Heat tolerance identification in different years and different ecological region is heat resistance kind, then is stable
Heat resistance kind.Mass of 1000 kernel heat susceptibility index is more than Jimai 20 in Heat Tolerance of Wheat Cultivars identification in 2013-2014 year intelligent greenhouses
1, but heat Heat Tolerance of Wheat Cultivars identification in canopy, 2014-2015 year intelligent greenhouse heat resistances mirror in the year crop fields 2013-2014
Fixed, the year crop fields 2014-2015 heat that the experiment of canopy heat tolerance identification, that the year different ecological regions 2015-2016 crop field heats canopy is resistance to
Mass of 1000 kernel heat susceptibility index is less than 1 in hot qualification test, is more stable heat resistance kind.Jimai 22 is in 2013-2014 years
Heat Tolerance of Wheat Cultivars identification, 2014-2015 year intelligent greenhouses Heat tolerance identification, the year crop fields 2014-2015 increase in intelligent greenhouse
Warm canopy heat tolerance identification experiment mass of 1000 kernel heat susceptibility index is less than 1, but heats Heat Tolerance of Wheat Cultivars in canopy in the year crop fields 2013-2014
Identification, the year different ecological regions 2015-2016 crop field heat mass of 1000 kernel heat susceptibility index in the experiment of canopy heat tolerance identification and are more than 1, then
It is not stable heat resistance kind.
Mass of 1000 kernel heat susceptibility index is less than the preferred embodiment that 1 the above is only this patent, it is noted that for this skill
For the those of ordinary skill in art field, under the premise of not departing from the art of this patent principle, can also make it is several improvement and
It replaces, these improve and replace the protection domain that also should be regarded as this patent.
Claims (7)
1. a kind of method of Heat Tolerance of Wheat Cultivars identification, includes the following steps：
（1）Intelligent greenhouse Heat tolerance identification：Wheat seed low temperature vernalization treatment is seeded in intelligent greenhouse, after 40 days round the clock
Temperature be set as 20/16 DEG C and round the clock when a length of 16/8h under the conditions of plant, florescence mark stem bloom fringe and the same day it is listed, Hua Hou
Diurnal temperature is adjusted to 25/16 DEG C；10 days after spending, it is divided into high-temperature process group and control group：High-temperature process group daytime temperature is set as
33 to 39 DEG C, night temperature is set as 20 DEG C；Control group diurnal temperature is set as 25/16 DEG C；The material of each Temperature Treatment is planted respectively
In the different planter units of intelligent greenhouse, independent temperature control, until Wheat Development maturation；
（2）Crop field, which is built, heats canopy Heat tolerance identification：Crop field Heat tolerance identification experiment takes two factor split block designs, primary area heat-resisting
Stress treatment and control two are handled, kind random alignment in secondary area, 3 repetitions；
High temperature stress processing：It 20 days after wheat flower, that is, is in the milk the later stage, under fine day or cloudy weather, is heated in what is built
Progress high temperature stress in canopy, daily high temperature stress 5 hours, i.e., 11:00-16:00；
Control treatment is cultivated by normal condition；
（3）Heat tolerance identification index records and heat susceptibility index calculates：Flowering stage of wheat, maturity period, boot leaf death phase are recorded, is measured
Mass of 1000 kernel；Grain-filling period is observed, heat susceptibility index S is calculated；
S=(1−YD/YP)/(1−)；
Wherein, YD is mass of 1000 kernel or Grain-filling period of certain wheat breed under high-temperature process or heat stress, and YP is that certain kind exists
Mass of 1000 kernel under normal environment or Grain-filling period, for all kinds mass of 1000 kernel or grouting under high-temperature process or heat stress processing
The average value of duration, for all kinds average value of mass of 1000 kernel or Grain-filling period in normal circumstances；
According to heat susceptibility index formula, mass of 1000 kernel heat susceptibility index and Grain-filling period heat susceptibility index are calculated；
（4）Heat resistance judges：Mass of 1000 kernel heat susceptibility index S ＜ 1 are then heat resistance kind；Mass of 1000 kernel heat susceptibility index S >=1 is then heat
Sensitive varieties；
In the heat resistance kind of mass of 1000 kernel heat susceptibility index S ＜ 1, the kind of Grain-filling period heat susceptibility index S ＜ 1, heat resistance
It is stronger；
（5）The judgement of stable heat resistance kind：Intelligent greenhouse Heat tolerance identification is heat resistance kind, and different year and not
With the crop field of ecological zone, to heat Heat tolerance identification in canopy be heat resistance kind, then is stable heat resistance kind.
2. a kind of method of Heat Tolerance of Wheat Cultivars identification according to claim 1：It is characterized in that the step（1）Intelligent temperature
Indoor Heat tolerance identification, the temperature of heat treatment be set as 33 DEG C, 35 DEG C, 37 DEG C, 39 DEG C or in which combination.
3. a kind of method of Heat Tolerance of Wheat Cultivars identification according to claim 2：It is characterized in that the step（1）Intelligent temperature
The temperature of indoor Heat tolerance identification, heat treatment is 35 DEG C.
4. according to a kind of method of Heat Tolerance of Wheat Cultivars identification of claim 1-3 any one of them：It is characterized in that the step
（2）Crop field builds to heat canopy Heat tolerance identification and choose, and there is representative 2 of planting area or more testing site to be identified.
5. a kind of method of Heat Tolerance of Wheat Cultivars identification according to claim 4：It is characterized in that the step（2）It takes in crop field
It builds and heats canopy Heat tolerance identification and carry out 2 years or more identifying.
6. a kind of method of Heat Tolerance of Wheat Cultivars identification according to claim 5：It is characterized in that this identification is suitable for the Yellow River and Huai He River
Sea region Heat Tolerance of Wheat Cultivars is identified.
7. a kind of method of Heat Tolerance of Wheat Cultivars identification according to claim 6：It is characterized in that identification place is the same as year point
Not in Jinan, Shandong Province, Hefei ,Anhui, Zhengzhou, henan and Hebei Shijiazhuang.
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| CN111869523A (en) * | 2020-08-10 | 2020-11-03 | 河北省农林科学院遗传生理研究所（河北省农林科学院农产品质量安全研究中心） | Method for identifying heat resistance of wheat in seedling stage |
| CN112616587A (en) * | 2020-12-21 | 2021-04-09 | 河北省农林科学院旱作农业研究所 | Method for selecting heat-resistant wheat variety by using grain weight heat-resistant index |
| CN113099982A (en) * | 2021-04-09 | 2021-07-13 | 安徽农业大学 | Method for improving stress tolerance of wheat at warming in grouting stage and application thereof |
| CN113826520B (en) * | 2021-09-30 | 2022-08-16 | 五河县绿色农产品协会 | Method for screening stable-yield wheat varieties with strong tolerance |
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