CN107873433A - A kind of authentication method of wheat leaf blade high temperature stress resistance - Google Patents

A kind of authentication method of wheat leaf blade high temperature stress resistance Download PDF

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Publication number
CN107873433A
CN107873433A CN201711019994.1A CN201711019994A CN107873433A CN 107873433 A CN107873433 A CN 107873433A CN 201711019994 A CN201711019994 A CN 201711019994A CN 107873433 A CN107873433 A CN 107873433A
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China
Prior art keywords
wheat
blade
date
withered
high temperature
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CN201711019994.1A
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Chinese (zh)
Inventor
孙道杰
张玲丽
冯毅
许小宛
高雅洁
姚俭昕
王辉
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Northwest A&F University
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Northwest A&F University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general

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  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Environmental Sciences (AREA)
  • Measuring Temperature Or Quantity Of Heat (AREA)

Abstract

The invention discloses a kind of authentication method of wheat leaf blade high temperature stress resistance, it is related to wheat growing technique field.The inventive method comprises the following steps:In Huang-Huai Area while sow in spring wheat breed and check variety to be identified;Observe each kind blade and hold green situation, the withered date when blade of record wheat breed and check variety is withered respectively;The withered date of wheat breed and the withered date of check variety are contrasted to determine that the high temperature resistance of wheat breed coerces performance;Wherein, the withered date is that the overall blade of plant holds corresponding date when green area ratio is down to blade whole area 20%;The check variety is No.6 Xiaoyan wheat, the wheat of all wheats 22 and the wheats of Xi Nong 979.The present invention authentication method can quickly, the high temperature stress resistance of precise Identification Huang-Huai Area wheat leaf blade.

Description

A kind of authentication method of wheat leaf blade high temperature stress resistance
Technical field
The present invention relates to wheat growing technique field, more particularly to a kind of identification side of wheat leaf blade high temperature stress resistance Method.
Background technology
With global warming, high temperature turns into an important factor for threatening Wheat Production.Wheat originally be happiness cool intolerant to The crop of high temperature, high temperature are easily caused wheat leaf blade function reduction or even completely lost.But to the patience of high temperature between different genotype With larger difference, the important research of wheat breeding project is all over the world turned into so cultivating and screening resistant to elevated temperatures wheat breed Target.Huang-Huai Area is China wheat main producing region, and it is current and future Wheat Production to cultivate and promote the good kind of heat resistance Need the urgent task solved as early as possible.However, for the heat resistance of kind, there is presently no accurately and rapidly evaluation method.It is raw In production it is to be understood that kind whether high temperature resistant is, it is necessary to which the production practices by more ground for many years are judged to examine.
Existing high temperature resistant authentication method mainly has three:
First, greenhouse identification method:High temperature stress identification is carried out using controllable greenhouse, in the different phase of wheat growth, is created The greenhouse of high temperature stress, heat resistance is evaluated according to wheat leaf blade function, growing way, yield.Greenhouse identification method is confined to In:1. greenhouse easily causes Growth of Wheat lopsided, growth potential is weak, and qualification result is difficult to the true spy for reflecting kind Property;2. greenhouse and crop field heat stress environment (soil temperature and humidity, Atmosphere temp.and RH, temperature and illumination variation situation, wind speed etc. Factor) differ greatly, qualification result poor reliability;3. greenhouse evaluation program is cumbersome, cost is high.
Second, field evening broadcasts identification method:This method is mainly suitable for low latitudes spring wheat area, and being broadcast by evening makes wheat growth Development later stage is in hot environment, according to the heat resistance of the character Indirect evaluation kinds such as its setting percentage, grain weight, yield.Currently Field evening broadcast the limitation of identification method and be:1. being appropriate only for spring varieties identification, winter wheat is difficult to normal development maturation, it is impossible to Grain characters and yield data are obtained, so winter habit, semi-winterness kind are not suitable for this method;2. field evening, which broadcasts method, is only suitable for the summer Area identification less high Ji Wendu, China Huang-Huai Area are in the maximum monsoon climate zone in the whole world, and the end of spring and the beginning of summer heating is too fast, The high temperature part in seven Augusts often leads to wheat death, and institute is in this way in Huang-Huai Area poor feasibility.
Third, physiological and biochemical index identification method:Because many physiological and biochemical indexs of wheat and the relation of heat resistance are not yet ground Study carefully it is clear, institute be in the exploratory stage mostly in this way.
The content of the invention
In view of this, the embodiments of the invention provide a kind of authentication method of wheat leaf blade high temperature stress resistance, main mesh Be quick and precisely identify Huang-Huai Area wheat leaf blade high temperature stress resistance.
To reach above-mentioned purpose, invention broadly provides following technical scheme:
On the one hand, the embodiments of the invention provide a kind of authentication method of wheat leaf blade high temperature stress resistance, methods described Comprise the following steps:
In Huang-Huai Area while sow in spring wheat breed and check variety to be identified;
Observe each kind blade and hold green situation, the withered day when blade of record wheat breed and check variety is withered respectively Phase;
The withered date of wheat breed and the withered date of check variety are contrasted to determine that the high temperature resistance of wheat breed is coerced Performance;
Wherein, the withered date is that the overall blade of plant is held when green area ratio is down to blade whole area 20% Corresponding day
Phase;The check variety is No.6 Xiaoyan wheat, the wheat of all wheats 22 and the wheats of Xi Nong 979.
Preferably, selected when wheat is sowed in Huang-Huai Area it is open, divulge information, be not easy the even uniform that is influenceed by subenvironment Experimental field.
Preferably, the time of the spring sowing is April 1 to April 5.
Preferably, the sowing line number for sowing in spring wheat breed and check variety to be identified is 3-5 rows, line space is equal For 30-35cm, strain spacing is 3-5cm.
Preferably, the withered date of the contrast wheat breed and the withered date of check variety are to determine wheat breed High temperature resistance stress performance detailed process be:
Dried up the date earlier than the blade of the western wheat of agriculture 979 when the blade of the wheat to be identified withered date, then institute The heat resistance for stating wheat breed to be identified is poor;
Dried up the date when the blade of the wheat to be identified withered date is equivalent or is later than the blade of the wheat of western agriculture 979 And dried up the date earlier than the blade of all wheats of wheat 22, then the heat resistance of the wheat breed to be identified is medium;
Dried up the date when the blade of the wheat to be identified withered date is equivalent or is later than the blade of the wheat of all wheats 22 And dried up the date earlier than the blade of the No.6 Xiaoyan wheat, then the heat resistance of the wheat breed to be identified is good;
When the blade of the wheat to be identified withered date is later than or is dried up day equal to the blade of the No.6 Xiaoyan wheat Phase, then the heat resistance of the wheat breed to be identified is outstanding.
Compared with prior art, the beneficial effects of the invention are as follows:
The present invention is directed to Huang-Huai Area wheat growth situation, using wheat to be identified and expert of the art and the kind of sowing in spring simultaneously Plant personnel are widely recognized as and the check variety of stability of characteristics, and select the wheat product of three kinds of different leaves high temperature stress resistance class Kind be used as check variety, then observe the blade of each kind and hold green phenomenon and record the blade withered date to determine wheat leaf to be identified The high temperature stress resistance of piece belongs to the technological means of which grade, using identification of means can quickly, precise Identification Huang-Huai Area it is small The high temperature stress resistance of wheat blade.
Brief description of the drawings:
Fig. 1 is the material object after the outstanding heat resistance wheat planting of the according to embodiments of the present invention 1 authentication method screening provided Figure;(what it is with arrow mark is the outstanding wheat plant growing way phenomenon of the heat resistance through screening, at the same time the phase, and the wheat of screening is planted Strain blade is all green substantially, and its left and right sides is the wheat plant growing way phenomenon of general kind, and its blade is substantially complete Dry up yellowly in portion)
Fig. 2 be it is according to embodiments of the present invention 1 provide authentication method screening outstanding heat resistance wheat planting after it is another Pictorial diagram.(what it is with arrow mark is the outstanding wheat plant growing way phenomenon of the heat resistance through screening, at the same time the phase, screening it is small Wheat plant leaf is all green substantially, and its left and right sides is the wheat plant growing way phenomenon of general kind, and its blade is substantially Through all withered yellowlies)
Embodiment
Be further illustrate the present invention to reach the technological means and effect that predetermined goal of the invention is taken, below with compared with Good embodiment, to embodiment, technical scheme, feature and its effect according to the present patent application, describe in detail as after.Under State it is bright in multiple embodiments in special characteristic, structure or feature can be combined by any suitable form.
Embodiment 1
Identify Huang-Huai Area wheat leaf blade high temperature stress resistance:Select it is open, divulge information, be not easy to be influenceed by subenvironment it is smooth Even test;Sow in spring April 1 time to April 5, sowed in spring simultaneously in Huang-Huai Area wheat breed to be identified, No.6 Xiaoyan are small Wheat, the wheat of all wheats 22 and the wheat of western agriculture 979;Each kind material sowing 3-5 rows, line space 30-35cm, strain spacing 3-5cm;See Examine each kind blade and hold green situation, the withered date when blade of record wheat breed and check variety is withered respectively is (above-mentioned dry The withered date refers to that the overall blade of plant holds corresponding date when green area ratio is down to blade whole area 20%);
The kind with stability characteristic (quality) that check variety is widely recognized as everybody, can be as tester;Lay down 6 for example, small Number heat resistance it is outstanding, rank be 1 grade, the heat resistance of all wheats 22 is good, rank be 2 grades, the heat resistance of western agriculture 979 is medium, level Wei not be 3 grades;Judge to dry up the date earlier than the blade of the above-mentioned western wheat of agriculture 979 when the blade of the above-mentioned wheat to be identified withered date, Then the heat resistance of above-mentioned wheat breed to be identified is poor;When the blade of above-mentioned wheat to be identified withered date and above-mentioned western agriculture 979 The blade of the wheat withered date is identical or between the blade of the western wheat of agriculture 979 and the wheat of all wheats 22 dries up the date, then above-mentioned The heat resistance of wheat breed to be identified is medium;When the blade of above-mentioned wheat to be identified withered date and above-mentioned all wheats of wheat 22 The blade withered date is identical or between the blade of all wheats of wheat 22 and No.6 Xiaoyan wheat dries up the date, then above-mentioned to be identified The heat resistance of wheat breed is good;It is later than or small equal to above-mentioned No.6 Xiaoyan when the blade of above-mentioned wheat to be identified date of drying up The blade of wheat dries up the date, then the heat resistance of above-mentioned wheat breed to be identified is outstanding.
The present invention is directed to Huang-Huai Area wheat growth situation, using the wheat and check variety to be identified of sowing in spring simultaneously, passes through The blade for observing each kind holds green phenomenon and records the blade withered date to determine the high temperature stress resistance of wheat leaf blade to be identified, The inventive method can quickly, the high temperature stress resistance of precise Identification Huang-Huai Area wheat leaf blade, can be wheat planting screen it is heat-resisting The outstanding kind of property, promote the yield and quality of wheat planting;As depicted in figs. 1 and 2, it is to use the inventive method evaluation and screening Plant growing way situation after the outstanding wheat breed sowing plantation of the Huang-Huai Area blade high temperature stress resistance that goes out, its left and right sides For the plant strain growth situation of general heat resistance, by contrast, it is evident that the high temperature resistance super identified using the present processes Property wheat growing way it is preferable, (leaf color can not be seen in figure, but actual conditions are in identical growth period, present invention sieve The good wheat leaf blade of the high-temperature stability of choosing is green entirely substantially, and other wheat colors of its both sides are substantially all withered Turn yellow), illustrate that the high temperature resistant stress resistance of the wheat breed blade of screening is very good, also further illustrate the identification side of the present invention Method is very reliable, and the plantation that can be chosen seeds really for wheat provides accurate feasible method.
Disclosed above is only the embodiment of the present invention, but protection scope of the present invention is not limited thereto, and is appointed What those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, all should It is included within the scope of the present invention.Therefore, protection scope of the present invention should using above-mentioned scope of the claims as It is accurate.

Claims (5)

1. a kind of authentication method of wheat leaf blade high temperature stress resistance, it is characterised in that the described method comprises the following steps:
In Huang-Huai Area while sow in spring wheat breed and check variety to be identified;
Observe each kind blade and hold green situation, the withered date when blade of record wheat breed and check variety is withered respectively;
The withered date of wheat breed and the withered date of check variety are contrasted to determine that the high temperature resistance of wheat breed coerces performance;
Wherein, the withered date is that the overall blade of plant holds correspondence when green area ratio is down to blade whole area 20% Date;The check variety is No.6 Xiaoyan wheat, the wheat of all wheats 22 and the wheats of Xi Nong 979.
2. the authentication method of a kind of wheat leaf blade high temperature stress resistance according to claim 1, it is characterised in that in the Yellow River and Huai He River Area sowing wheat when select it is open, divulge information, be not easy the even uniform influenceed by subenvironment experimental field.
A kind of 3. authentication method of wheat leaf blade high temperature stress resistance according to claim 1, it is characterised in that the spring The time broadcast is April 1 to April 5.
A kind of 4. authentication method of wheat leaf blade high temperature stress resistance according to claim 1, it is characterised in that the spring The sowing line number for broadcasting wheat breed and check variety to be identified is 3-5 rows, and line space is 30-35cm, and strain spacing is 3- 5cm。
5. the authentication method of a kind of wheat leaf blade high temperature stress resistance according to claim 1, it is characterised in that described right Than the withered date of wheat breed and the withered date of check variety to determine the specific of the high temperature resistance of wheat breed stress performance Process is:
Dried up the date earlier than the blade of the western wheat of agriculture 979 when the blade of the wheat to be identified withered date, then it is described to treat Identify that the heat resistance of wheat breed is poor;
Dried up date and morning when the blade of the wheat to be identified withered date is equivalent or is later than the blade of the wheat of western agriculture 979 Dried up the date in the blade of all wheats of wheat 22, then the heat resistance of the wheat breed to be identified is medium;
Dried up date and morning when the blade of the wheat to be identified withered date is equivalent or is later than the blade of the wheat of all wheats 22 Dried up the date in the blade of the No.6 Xiaoyan wheat, then the heat resistance of the wheat breed to be identified is good;
When the wheat to be identified blade dry up the date be later than or equal to the No.6 Xiaoyan wheat blade dry up the date, then The heat resistance of the wheat breed to be identified is outstanding.
CN201711019994.1A 2017-10-27 2017-10-27 A kind of authentication method of wheat leaf blade high temperature stress resistance Pending CN107873433A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108918578A (en) * 2018-07-04 2018-11-30 山东省农业科学院作物研究所 A kind of Heat Tolerance of Wheat Cultivars brief appraisal method
CN109006264A (en) * 2018-06-17 2018-12-18 河北省农林科学院旱作农业研究所 New wheat breed dry heat resistance wind identification method and Special temp controlling canopy
CN113170669A (en) * 2021-05-10 2021-07-27 河北省农林科学院旱作农业研究所 Pre-identification and selection method of wheat material with high water-saving index

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105557487A (en) * 2015-12-22 2016-05-11 山东农业大学 Test method for studying high-temperature stress on wheat
CN106526083A (en) * 2016-11-08 2017-03-22 山东省农业科学院作物研究所 A wheat heat resistance identification method
CN106613923A (en) * 2016-11-29 2017-05-10 南京农业大学淮安研究院 Method for screening plant variety with leaf strengthening function

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105557487A (en) * 2015-12-22 2016-05-11 山东农业大学 Test method for studying high-temperature stress on wheat
CN106526083A (en) * 2016-11-08 2017-03-22 山东省农业科学院作物研究所 A wheat heat resistance identification method
CN106613923A (en) * 2016-11-29 2017-05-10 南京农业大学淮安研究院 Method for screening plant variety with leaf strengthening function

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109006264A (en) * 2018-06-17 2018-12-18 河北省农林科学院旱作农业研究所 New wheat breed dry heat resistance wind identification method and Special temp controlling canopy
CN108918578A (en) * 2018-07-04 2018-11-30 山东省农业科学院作物研究所 A kind of Heat Tolerance of Wheat Cultivars brief appraisal method
CN113170669A (en) * 2021-05-10 2021-07-27 河北省农林科学院旱作农业研究所 Pre-identification and selection method of wheat material with high water-saving index
CN113170669B (en) * 2021-05-10 2022-12-16 河北省农林科学院旱作农业研究所 Pre-identification and selection method of wheat material with high water-saving index

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Application publication date: 20180406