CN106526083A - A wheat heat resistance identification method - Google Patents
A wheat heat resistance identification method Download PDFInfo
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- CN106526083A CN106526083A CN201610976555.9A CN201610976555A CN106526083A CN 106526083 A CN106526083 A CN 106526083A CN 201610976555 A CN201610976555 A CN 201610976555A CN 106526083 A CN106526083 A CN 106526083A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
A wheat heat resistance identification method is provided. The method includes identifying heat resistance in an intelligent greenhouse, identifying heat resistance in a greenhouse built in land for growing field crops, recording heat resistance identification indexes, calculating a thermal inductance index, determining variety heat resistance, determining a stable heat resistant variety, and other steps. Identification results of the method are accurate and reliable. According to the method, heat resistance identification in the intelligent greenhouse and heat resistance identification in the greenhouse built in land for growing field crops are combined, and interannual heat resistance identification results and heat resistance identification results of different ecoregions are compared and combined, and therefore the method is of great significance for heat resistance identification of wheat genetic resources and breeding parents, for screening of proper heat resistant wheat varieties suitable for plantation in Huanghuaihai zones and for grain safety in China. The identification indexes are easy to measure, and through mutual corroboration of indoor identification and land identification, heat resistance of a wheat variety can be truly reflected.
Description
Technical field
The testing indexs such as heat susceptibility index are the present invention relates to the use of, the physiological and biochemical procedure of Heat Tolerance of Wheat Cultivars is studied, is especially related to
And a kind of method of Heat Tolerance of Wheat Cultivars identification.
Background technology
Wheat is the important cereal crops of China, belongs to the cool habit of happiness, within the season of growth, particularly has important with yield
The pustulation period of relation, it is easy to affected by the heat stress that abnormal high temperature weather is caused, yield is caused to decline, quality is reduced.
Often there is hot dry wind in the wheat belt such as the Yellow River and Huai He River sea region and Xinjiang, Wheat in Grain Filling Stage Jing, and sometimes up to more than 32 DEG C of temperature is caused
Wheat yield 10-20%.Northern China and middle and lower reach of Yangtze River Winter Wheat Area are also it occur frequently that high temperature damage, causes wheat yield.
Identification, screening wheat heat resistant variety and germ plasm resource, it is to solve Heat Tolerance of Wheat Cultivars to carry out Heat Tolerance of Wheat Cultivars breeding
Fundamental way.Plantation adapts to the wheat breed of high temperature stress, is the important channel for realizing and keeping Yield Potential of Wheat.
Existing heat-resisting Journal of Sex Research, lays particular emphasis on more and builds plastic greenhouse by land for growing field crops and carry out heat-resistance test, but single land for growing field crops is resistance to
Heat research is often affected by factors such as weather, environment, wheat growth cycles, and its heat resistance result of study is often unstable
Fixed, the heat resistant variety year of identification, difference was larger and between zones of different between border, it is impossible to accurately reflect the heat resistance of kind.The present invention will
The Heat tolerance identification that the Heat tolerance identification of controllable temperature is heated with land for growing field crops in canopy in intelligent greenhouse combines, by intelligent greenhouse and
The mutual confirmation of land for growing field crops kind Heat tolerance identification result, further increases the accuracy of wheat breed Heat tolerance identification, and this is resistance to
Hot authentication method, there is not been reported both at home and abroad at present.
The content of the invention
To solve technical deficiency above, the invention provides a kind of method of Heat Tolerance of Wheat Cultivars identification.
The present invention is achieved by the following technical solutions：
A kind of method of Heat Tolerance of Wheat Cultivars identification that the present invention is provided, comprises the following steps：
（1）Heat tolerance identification in intelligent greenhouse：Wheat seed low temperature vernalization treatment 40 days, sows in intelligent greenhouse, round the clock
Temperature is set to 20/16 DEG C and duration is planted under the conditions of being set to 16/8h round the clock, and florescence mark stem is bloomed, and simultaneously the same day is listed fringe, flower
Diurnal temperature is adjusted to 25/16 DEG C afterwards；10 days after spending, it is divided into high-temperature process group and control group；High-temperature process group, at daytime high temperature
Reason temperature is set to 33 to 39 DEG C, and night temperature is set to 20 DEG C；Control group, diurnal temperature are set to 25/16 DEG C；The material of each Temperature Treatment
Material is planted in the different planter units of intelligent greenhouse respectively, independent temperature control, until Growth of Wheat is to maturation；
（2）Build and heat Heat tolerance identification in canopy in land for growing field crops：Land for growing field crops Heat tolerance identification test, takes two factor split block designs, primary area
Two process of heat-resisting Stress treatment and control, kind random alignment in secondary area, 3 repetitions；
High temperature stress process：20 days after wheat flower, that is, it is in the milk the later stage, under fine day or cloudy weather, in heating for building
Carry out high temperature stress in canopy, daily high temperature stress 5 hours, i.e., 11:00-16:00；
Control treatment, is cultivated by normal condition；
（3）Heat tolerance identification index is recorded and heat susceptibility index is calculated：Record flowering stage of wheat, maturity period, boot leaf death phase, determine
Mass of 1000 kernel；Calculate Grain-filling period and heat susceptibility index S；
S=(1−YD/YP)/(1−/)；
Wherein, YD is mass of 1000 kernel or Grain-filling period of certain wheat breed under high-temperature process or heat stress, and YP exists for certain kind
Mass of 1000 kernel or Grain-filling period under home,For all kinds in the case where high-temperature process or heat stress are processed mass of 1000 kernel or
The mean value of Grain-filling period,For the mean value of all kinds mass of 1000 kernel or Grain-filling period in normal circumstances；
According to heat susceptibility index formula, mass of 1000 kernel heat susceptibility index and Grain-filling period heat susceptibility index are calculated；
（4）Heat resistance judges：Mass of 1000 kernel heat susceptibility index S ＜ 1, then be heat resistance kind；Mass of 1000 kernel heat susceptibility index S >=1, then be heat
Sensitive varieties；
In the heat resistance kind of mass of 1000 kernel heat susceptibility index S ＜ 1, Grain-filling period heat susceptibility index S ＜'s 1, then kind heat resistance
It is higher；
（5）The judgement of stable heat resistance kind：Intelligent greenhouse Heat tolerance identification is heat resistance kind, and different year and not
It is heat resistance kind that Heat tolerance identification in canopy is heated with the land for growing field crops of ecological zone, then be stable heat resistance kind.
A kind of method of Heat Tolerance of Wheat Cultivars identification that the present invention is provided：The step（1）Heat tolerance identification in intelligent greenhouse,
Heat treatment preferable temperature is set to 33 DEG C, 35 DEG C, 37 DEG C, 39 DEG C or combination therein.
A kind of method of Heat Tolerance of Wheat Cultivars identification that the present invention is provided：The step（1）Heat tolerance identification in intelligent greenhouse,
Preferred temperature is heat-treated for 35 DEG C.
A kind of method of Heat Tolerance of Wheat Cultivars identification that the present invention is provided：The step（2）Land for growing field crops heats heat resistance mirror in canopy
Fixed preferably selection is identified with representational more than 2 testing sites of planting area.
A kind of method of Heat Tolerance of Wheat Cultivars identification that the present invention is provided：The step（2）Land for growing field crops heats heat resistance mirror in canopy
Surely carry out preferably more than 2 years identifying.
A kind of method of Heat Tolerance of Wheat Cultivars identification that the present invention is provided：It is resistance to that this identification is preferably applied to the Yellow River and Huai He River sea region wheat
Hot identification.
A kind of method of Heat Tolerance of Wheat Cultivars identification that the present invention is provided：Identification place is preferably with year in Jinan, Shandong Province, peace
Emblem Hefei, Zhengzhou, henan and Hebei Shijiazhuang.
The invention has the beneficial effects as follows：The Heat tolerance identification method that the present invention is provided, qualification result is accurately and reliably.The present invention
Built by identification and land for growing field crops in intelligent greenhouse and heat in canopy identification and combine, year border heat tolerance identification results contrast and different ecological
Region heat tolerance identification results contrast combines, reasonable selection heat tolerance identification region, to the resistance to of Wheat Germplasm Resources and breeding parent
The hot heat-resisting wheat breed identified and screen suitable Huang-Huai-Hai area of wheat plantation, improves wheat yield, ensures national food peace
It is complete significant.The identification of indicator that the present invention chooses easily is measured, and is mutually confirmed with land for growing field crops identification by indoor identification, energy
The heat resistance of enough true reflection wheat breeds.
Description of the drawings
Fig. 1 heats canopy.
Specific embodiment
Further detailed description is done to the present invention with reference to embodiment：
Heat Tolerance of Wheat Cultivars identification in 1 2013-2014 of embodiment year intelligent greenhouses
Carry out in the intelligent greenhouse that Crop Inst. of shandong Prov. Agriculture science Academy builds.On November 18th, 2013, to wheat product
Planting carries out low temperature vernalization.After vernalization 40 days, each wheat breed respectively sows 20 basins, plants 4 plants per basin.Put diurnal temperature and round the clock
Duration is set to 20/16 DEG C, grows under 16/8h.Florescence mark stem is bloomed, and simultaneously the same day is listed fringe.Meanwhile, the growth temperature of Post flowering
Degree and round the clock duration are adjusted to 25/16 DEG C, 16/8h.10 days after spending, each kind takes 10 cone materials and moves to growth temperature and round the clock
Duration is set to 33/20 DEG C, carries out high-temperature process in the greenhouse unit of 16/8h, until reaching maturity.
Result of the test shows, under the conditions of high-temperature process, main Ear weight, single-strain grain weight, mass of 1000 kernel and Grain-filling period with not
Significant difference between process（Table 1）.By heat susceptibility index as can be seen that the wheat product of mass of 1000 kernel and Grain-filling period heat susceptibility index S ＜ 1
Kind（System）Respectively Jimai 22, Jimai 23, Lankao 906-4 and LS6109（Table 2）.
1 2013-2014 of table year intelligent greenhouse wheat Themotalerance difference analysis
| Proterties | Normal process average | Heat treatment average |
| Main grain number per spike | 45.5a | 45.9a |
| Main Ear weight | 1.96a | 1.8b |
| Single-strain grain weight | 3.96a | 3.0b |
| Single-strain grain number | 90.8a | 85.3a |
| Mass of 1000 kernel | 43.5a | 35.3b |
| Grain-filling period | 39.95a | 30.7b |
2 2013-2014 of table year intelligent greenhouse wheat breed Themotalerances are determined and its heat susceptibility index
2 2013-2014 of embodiment year lands for growing field crops heat Heat Tolerance of Wheat Cultivars identification in canopy
Testing was carried out in Crop Inst. of shandong Prov. Agriculture science Academy experimental plot in 2013-2014 years.Each kind is carried out
Cell is planted, and 6 rows are planted per area, and row is long 2 meters.Two factor split block designs are taken, different cultivars completely random arrangement in district's groups.Flower
10 days afterwards, build in high-temperature process growing area and heat canopy（Fig. 1）, carry out resistance to heat treatment.By heat susceptibility index as can be seen that mass of 1000 kernel
With the wheat breed of Grain-filling period heat susceptibility index S ＜ 1（System）Respectively Jimai 35, Jimai 20, Jimai 27 and Shandong original 502（Table
3）.
3 2013-2014 of table year lands for growing field crops heat canopy wheat breed Themotalerance and determine and its heat susceptibility index
3 2014-2015 of embodiment year intelligent greenhouse Heat tolerance identifications
Carry out in Crop Inst. of shandong Prov. Agriculture science Academy's intelligent greenhouse.First test material is carried out in vernalization room low
Warm vernalization, then carries out potted plant growth.Diurnal temperature before blooming is set to 20/16 DEG C；The diurnal temperature of Post flowering is set to
35/20 DEG C, 37/20 DEG C and 39/20 DEG C three heat stress temperature, high temperature stress process ripe to wheat seed.
As can be seen from Table 4, the main Ear weight for the treatment of of different temperature, single-strain grain weight, mass of 1000 kernel and Grain-filling period are right
According to there is pole significant difference between different disposal.The basic trend of three high-temperature process results is that temperature is higher, and material accumulation is got over
Few, main Ear weight, single-strain grain weight, mass of 1000 kernel are lower, and Grain filling duration is shorter.Mass of 1000 kernel under 37 DEG C of process is processed than 35 DEG C
Under it is slightly higher, single-strain grain weight is slightly higher, but be not up to significant difference.When illustrating that temperature reaches 35 DEG C, wheat is produced
Very serious high temperature stress.When carrying out high temperature resistant research in the intelligent greenhouse, completely may be used using 35 DEG C as high temperature stress temperature
To reach heat-resisting research purpose.
In this research, 39 DEG C of Temperature Treatment is maximum with contrast difference, therefore when carrying out heat susceptibility index calculating, with 39 DEG C
Each index is calculated for stress index.By the heat-resistance index for calculating 4 indexs, wherein 3 kinds such as Jimais 20, Jimai 22
1 is respectively less than with the heat susceptibility index of all indexs of Jimai 23, illustrates that this 3 kinds have preferable heat resistance, and Jimai 20
Heat in intelligent greenhouse and land for growing field crops with Jimai 22 that qualification result in canopy is consistent, so as to further prove our heat tolerance identification results
Reliability.All heat susceptibility indexes of 4 kinds such as tobacco grower 19, Jimai 27, Jimai 35 and LS6109 are all higher than 1, illustrate theirs
Heat resistance is poor, and all indexs of wherein tobacco grower 19 are heated in land for growing field crops and be all higher than in canopy 1.The heat-resistance index of other 6 kinds, no
Have differences between index, illustrate that anti-perception falls between（Table 5）.
4 2014-2015 of table year intelligent greenhouse Themotalerance difference analysis
The measure of each Themotalerance in 5 2014-2015 of table year intelligent greenhouses
4 2014-2015 of embodiment year lands for growing field crops heat the test of canopy heat tolerance identification
Land for growing field crops Heat tolerance identification test is carried out in Crop Inst. of shandong Prov. Agriculture science Academy experimental plot, takes two factor split plots
Design, different cultivars completely random arrangement in district's groups.In Wheat Plant in Late Filling Period, heat canopy and carry out high temperature stress by building.
Land for growing field crops heats the heat resistant test in canopy and has mainly investigated the Grain-filling period of different disposal, mass of 1000 kernel, cell production
With 4 indexs of unit weight.The wherein Grain-filling period of Jimai 20 and Jimai 23, mass of 1000 kernel, the heat of 4 indexs of cell production and unit weight
Sense index is respectively less than 1, shows that the two kind heat resistances are preferable；The Grain-filling period of Jimai 2, mass of 1000 kernel, 3 fingers of cell production
Target heat susceptibility index is respectively less than 1, shows that the two kind heat resistances are preferable；The grouting of tobacco grower 19, Jimai 06037 and continent unit 9369
Duration, mass of 1000 kernel, the heat susceptibility index of 4 indexs of cell production and unit weight are all higher than 1, show this 3 kinds（System）It is heat-resisting
Property is poor.Remaining 7 kind, examines the heat susceptibility index of 4 different indexs, it is impossible to consistent more than or less than 1, illustrates these product
The heat resistance planted falls between（Table 6）.
6 2014-2015 of table year lands for growing field crops heat the heat susceptibility index of canopy Themotalerance
5 2015-2016 of embodiment year different ecological regions land for growing field crops heats the test of canopy heat tolerance identification
Land for growing field crops has been carried out in 4 planted in different ecological areas such as Jinan, Shandong Province, Zhengzhou, henan, Hefei ,Anhui and Hebei Shijiazhuang to heat in canopy
Heat tolerance identification.Land for growing field crops Heat tolerance identification test is taken at two factor split block designs, the heat-resisting Stress treatment in primary area and control two
Reason, kind random alignment in secondary area, 3 repetitions.20 days after wheat flower, that is, it is in the milk the later stage, under fine day or cloudy weather,
High temperature stress carry out in canopy being heated.Daily high temperature stress 5 hours, i.e., 11:00-16:00.
1. temperature statistics analysis
Heating in terms of canopy internal and external temperature statistics from 4 experimental points, builds heat-resisting canopy by land for growing field crops, 11：00-16：00 point
Time period carries out lid canopy, can be seen that to heat in canopy from temperature statistics result and can be higher by 20.7 DEG C compared with the outer temperature highest of canopy, table
It is bright by building gain of heat canopy, the hot environment in canopy can be realized（Table 7-10）.
7 Jinan, Shandong Province of table heats canopy internal and external temperature record sheet（℃）
8 Zhengzhou, henan of table heats canopy internal and external temperature record sheet（℃）
9 Hefei ,Anhui canopy internal and external temperature record sheet of table（℃）
10 Hebei Shijiazhuang of table heats canopy internal and external temperature record sheet（℃）
2. Grain-filling period or blade hold green phase analysis
Using portable Chlorophyll meter SPAD-502 plus, determine flowering stage of wheat and contain to the chlorophyll of maturity period boot leaf
Amount, calculates Grain-filling period or blade holds the green phase.
Measure period is divided into before high temperature stress and latter two period of high temperature stress.Minute before high temperature stress is to open
Flower, Post flowering the 5th day, the 10th day, the 15th day, the 20th day.After high temperature stress, the material in canopy is heated, was determined 1 time every 1 day,
Until wheat is ripe（The material of lid canopy is not compareed）.Each kind determines 10 plants every time, after removing measure peak, is averaging
Value.
Can be seen that from the measurement result of boot leaf SPAD 4 experimental points of value（Table 11-14）, after wheat flower when longer one section
In, boot leaf SPAD values are always maintained at more stable level, but later stage boot leaf SPAD value of being in the milk is gradually lowered, and most kinds exist
In the canopy of multiple testing sites, boot leaf SPAD values are less than the outer SPAD values of canopy, and with wheat grain filling process, its difference gradually increases, explanation
The heat-resisting canopy that testing site is built, generates heat stress to wheat, causes wheat early ageing phenomenon occur.
Impact of the high temperature stress to boot leaf SPAD values after 11 wheat flower of table（Hefei ,Anhui）
Impact of the high temperature stress to boot leaf SPAD values after 12 wheat flower of table（Zhengzhou, henan）
Impact of the high temperature stress to boot leaf SPAD values after 13 wheat flower of table（Hebei Shijiazhuang）
Impact of the high temperature stress to boot leaf SPAD values after 14 wheat flower of table（Jinan, Shandong Province）
3. changing bulk density analysis
Bulk Density of Wheat refers to quality of its seed in unit volume, with gram per liter（g/L）Represent, its size is by including seed
What many factors such as this body structure, moisture, impurity and unsound grain content were determined.In general, Grain Ripening is full, structure is tight
Close, particle is little, the sample that water content is low, and unit weight is larger；Conversely, unit weight is less.Therefore, the change of unit weight can be resistance to as wheat
One reference index of heat.The Volume-weight Determination result of three testing sites shows that Heat Treatment on Wheat grain test weight produces different journeys
The reduction of degree, it is 4.6-29.99% or so to reduce scope（Table 15）.
15 3 testing site Volume-weight Determination results of table
4. mass of 1000 kernel and volume analysis
Mass of 1000 kernel heat susceptibility index and yield heat susceptibility index are compared（Table 16, table 17）, find using mass of 1000 kernel heat susceptibility index
Judge the heat resistance of wheat breed, its result is more accurate, potential output due to being short of seedling disconnected ridge by cell, plot area measurement
Deng the impact of many factors, the larger event of error.
16 4 experimental point mass of 1000 kernel of table and heat susceptibility index
17 4 experimental point yield of table and heat susceptibility index
Note：Yield average 658.2 in 99 canopy of good star of Henan testing site, the outer yield average 649.9 of canopy, heat susceptibility index is -0.20,
Reason is that the outer material of canopy occurs lodging.
Comprehensive analysis above example, it can be seen that gain of heat canopy heat tolerance identification is built in intelligent greenhouse heat tolerance identification and land for growing field crops,
Qualification result has differences, and there is also difference between the annual result of land for growing field crops heat tolerance identification；Intelligent greenhouse Heat tolerance identification is anti-
Hot kind, and the land for growing field crops Heat tolerance identification in different year and different ecological region is heat resistance kind, then for stable
Heat resistance kind.The mass of 1000 kernel heat susceptibility index in Heat Tolerance of Wheat Cultivars identification in 2013-2014 year intelligent greenhouses of Jimai 20 is more than
1, but Heat Tolerance of Wheat Cultivars identification in canopy, 2014-2015 year intelligent greenhouse heat resistance mirror are heated in 2013-2014 year lands for growing field crops
Fixed, 2014-2015 year lands for growing field crops heat the test of canopy heat tolerance identification, that 2015-2016 year different ecological regions land for growing field crops heats canopy is resistance to
In hot qualification test, mass of 1000 kernel heat susceptibility index is less than 1, is more stable heat resistance kind.Jimai 22 is annual in 2013-2014
In intelligent greenhouse, Heat Tolerance of Wheat Cultivars identification, 2014-2015 year intelligent greenhouse Heat tolerance identifications, 2014-2015 year lands for growing field crops increase
Warm canopy heat tolerance identification test mass of 1000 kernel heat susceptibility index is less than 1, but heats Heat Tolerance of Wheat Cultivars in canopy in 2013-2014 year lands for growing field crops
Identification, 2015-2016 year different ecological regions land for growing field crops heat mass of 1000 kernel heat susceptibility index during canopy heat tolerance identification is tested and are more than 1, then
It is not stable heat resistance kind.
Mass of 1000 kernel heat susceptibility index less than 1 the above be only this patent preferred embodiment, it is noted that for this skill
For the those of ordinary skill in art field, on the premise of without departing from the art of this patent principle, can also make it is some improvement and
Replace, these improve and replacement also should be regarded as the protection domain of this patent.
Claims (7)
1. a kind of method of Heat Tolerance of Wheat Cultivars identification, comprises the following steps：
（1）Intelligent greenhouse Heat tolerance identification：Wheat seed low temperature vernalization treatment was seeded in intelligent greenhouse, round the clock after 40 days
Temperature be set to 20/16 DEG C and round the clock when a length of 16/8h under the conditions of plant, bloom fringe same day of florescence mark stem is listed, Hua Hou
Diurnal temperature is adjusted to 25/16 DEG C；10 days after spending, it is divided into high-temperature process group and control group：High-temperature process group daytime temperature is set to
33 to 39 DEG C, night temperature is set to 20 DEG C；Control group diurnal temperature is set to 25/16 DEG C；The material of each Temperature Treatment is planted respectively
In the different planter units of intelligent greenhouse, independent temperature control, until Wheat Development maturation；
（2）Build and heat canopy Heat tolerance identification in land for growing field crops：Two factor split block designs are taken in land for growing field crops Heat tolerance identification test, and primary area is heat-resisting
Two process of Stress treatment and control, kind random alignment in secondary area, 3 repetitions；
High temperature stress process：20 days after wheat flower, that is, it is in the milk the later stage, under fine day or cloudy weather, in heating for building
Carry out high temperature stress in canopy, daily high temperature stress 5 hours, i.e., 11:00-16:00；
Control treatment, is cultivated by normal condition；
（3）Heat tolerance identification index is recorded and heat susceptibility index is calculated：Record flowering stage of wheat, maturity period, boot leaf death phase, determine
Mass of 1000 kernel；Calculate Grain-filling period and heat susceptibility index S；
S=(1−YD/YP)/(1−/)；
Wherein, YD is mass of 1000 kernel or Grain-filling period of certain wheat breed under high-temperature process or heat stress, and YP exists for certain kind
Mass of 1000 kernel or Grain-filling period under home,For all kinds in the case where high-temperature process or heat stress are processed mass of 1000 kernel or
The mean value of Grain-filling period,For the mean value of all kinds mass of 1000 kernel or Grain-filling period in normal circumstances；
According to heat susceptibility index formula, mass of 1000 kernel heat susceptibility index and Grain-filling period heat susceptibility index are calculated；
（4）Heat resistance judges：Mass of 1000 kernel heat susceptibility index S ＜ 1, then be heat resistance kind；Mass of 1000 kernel heat susceptibility index S >=1, then be heat
Sensitive varieties；
In the heat resistance kind of mass of 1000 kernel heat susceptibility index S ＜ 1, the kind of Grain-filling period heat susceptibility index S ＜ 1, its heat resistance
It is higher；
（5）The judgement of stable heat resistance kind：Intelligent greenhouse Heat tolerance identification is heat resistance kind, and different year and not
It is heat resistance kind that Heat tolerance identification in canopy is heated with the land for growing field crops of ecological zone, then be stable heat resistance kind.
2. the method that a kind of Heat Tolerance of Wheat Cultivars according to claim 1 is identified：It is characterized in that the step（1）Intelligent temperature
Indoor Heat tolerance identification, the temperature of heat treatment are set to 33 DEG C, 35 DEG C, 37 DEG C, 39 DEG C or combination therein.
3. the method that a kind of Heat Tolerance of Wheat Cultivars according to claim 2 is identified：It is characterized in that the step（1）Intelligent temperature
Indoor Heat tolerance identification, the temperature of heat treatment is 35 DEG C.
4. the method that a kind of Heat Tolerance of Wheat Cultivars according to claim 1-3 is identified：It is characterized in that the step（2）Land for growing field crops
Building to heat canopy Heat tolerance identification and choose, there is representational more than 2 testing sites of planting area to be identified.
5. the method that a kind of Heat Tolerance of Wheat Cultivars according to claim 4 is identified：It is characterized in that the step（2）Take in land for growing field crops
Build heat canopy Heat tolerance identification carry out more than 2 years identify.
6. the method that a kind of Heat Tolerance of Wheat Cultivars according to claim 5 is identified：It is characterized in that this identification is applied to the Yellow River and Huai He River
Sea region Heat Tolerance of Wheat Cultivars is identified.
7. the method that a kind of Heat Tolerance of Wheat Cultivars according to claim 6 is identified：It is characterized in that identification place is with year point
Not in Jinan, Shandong Province, Hefei ,Anhui, Zhengzhou, henan and Hebei Shijiazhuang.
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| CN112616587A (en) * | 2020-12-21 | 2021-04-09 | 河北省农林科学院旱作农业研究所 | Method for selecting heat-resistant wheat variety by using grain weight heat-resistant index |
| CN113099982A (en) * | 2021-04-09 | 2021-07-13 | 安徽农业大学 | Method for improving stress tolerance of wheat at warming in grouting stage and application thereof |
| WO2023050728A1 (en) * | 2021-09-30 | 2023-04-06 | 五河县绿色农产品协会 | Method for screening wheat variety having strong tolerance and stable yield |
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