AU2021104130A4 - Method for identifying and evaluating drought resistance of peanuts by using compensatory growth rate - Google Patents

Method for identifying and evaluating drought resistance of peanuts by using compensatory growth rate Download PDF

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AU2021104130A4
AU2021104130A4 AU2021104130A AU2021104130A AU2021104130A4 AU 2021104130 A4 AU2021104130 A4 AU 2021104130A4 AU 2021104130 A AU2021104130 A AU 2021104130A AU 2021104130 A AU2021104130 A AU 2021104130A AU 2021104130 A4 AU2021104130 A4 AU 2021104130A4
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drought
soil
treatment
stage
water content
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Fengzhen Liu
Lu LUO
Qian Wan
Yongshan Wan
Kun Zhang
Xiurong Zhang
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Shandong Agricultural University
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    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/0098Plants or trees
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/13Abiotic stress
    • Y02A40/132Plants tolerant to drought

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Abstract

OF THE DISCLOSURE The invention discloses a method for identifying and evaluating the drought resistance of peanuts by using a compensatory growth rate, which comprises the following steps: sowing peanut materials to be evaluated (including varieties, new strains, breeding materials, germplasm materials, etc.), conducting drought treatment in key growth stages, and then conducting rewatering treatment; and taking normal and suitable water supply as a control, measuring plant biomass at the end of drought treatment (DW1) and 15 days after rewatering (DW2), conducting sampling for plant biomass measurement on the control too, respectively marking the plant biomass as WI and W2, calculating the compensatory growth rate, and evaluating the drought resistance of the peanut materials by using the compensatory growth rate. The method can quickly and accurately identify and evaluate the drought resistance of the peanut materials, is used for reflecting drought resistance, water use efficiency and yield potential, and significantly improves the efficiency and accuracy of drought-resistant peanut breeding.

Description

METHOD FOR IDENTIFYING AND EVALUATING DROUGHT RESISTANCE OF PEANUTS BY USING COMPENSATORY GROWTH RATE BACKGROUND OF THE INVENTION
[0001] 1. Technical Field
[0002] The invention relates to the field of agricultural science and farming
techniques, in particular to a method for identifying and evaluating the drought resistance
of peanuts by using a compensatory growth rate.
[0003] 2. Description of Related Art
[0004] The annual planting area of peanuts in China is over 70 million mu
(equivalent to 1/15 or about 0.066 hectares), with an average per unit yield of 250 kg/mu.
The total yield and export volume of peanuts in China rank first in the world,
outnumbering other domestic oil crops. Therefore, peanut planting plays an important role
in ensuring the safety of oil supply, promoting agricultural supply-side structural reform
and increasing farmers' income in China. Peanuts are mainly planted in hilly areas and flat
sandy land, drought stress is the main factor affecting the yield, and a drought-induced
crop failure of 30%-50% is often seen.
[0005] Cultivating new drought-resistant and water-saving peanut varieties is an
urgent need to reduce drought hazards and increase the yield. However, it is technically
difficult to accurately identify the drought resistance of new varieties and materials in the
process of breeding new varieties, which is an important technical problem in
drought-resistant peanut breeding.
[0006] Peanut drought resistance evaluation indicators are the key to
drought-resistant peanut breeding. The existing peanut drought resistance evaluation
indicators mainly include the drought resistance coefficient and the drought resistance
index. The drought resistance coefficient can not reflect the yield level of a variety under drought stress. Some varieties with high drought resistance coefficients have low yield levels under both wetness and drought conditions, and varieties with different production potential levels may have the same drought resistance coefficient. The drought resistance index incorporates a control variety as a reference, takes into account the absolute yield and drought resistance coefficient, and can evaluate the drought resistance and yielding ability of varieties. However, this evaluation is the result of the comprehensive action of various factors in the whole growth stage of peanuts, and can not reflect the adaptive response of peanuts to drought stress after drought stress in each growth stage.
BRIEF SUMMARY OF THE INVENTION
[0007] In view of the above-mentioned prior art, the purpose of the invention is to
provide a method for identifying and evaluating the drought resistance of peanuts by using
a compensatory growth rate. According to the invention, after drought treatment,
rewatering treatment is conducted, and the compensatory growth capacity of peanuts after
drought stress is evaluated by measuring plant biomass data and calculating the
compensatory growth rate, so that the drought resistance of new peanut varieties can be
quickly and accurately identified, the efficiency and accuracy of drought-resistant peanut
breeding are obviously improved, and water use efficiency and yield potential can be
reflected.
[0008] In order to achieve the above purpose, the invention adopts the following
technical solution:
[0009] A method for identifying and evaluating the drought resistance of peanuts
by using a compensatory growth rate comprises the following steps:
[0010] Conducting drought treatment on peanut materials to be evaluated in key
growth stages and rewatering after drought treatment; and taking normal water supply as a
control, calculating the compensatory growth rate by measuring plant biomass at the end of drought treatment and 15 days after rewatering, and evaluating the drought resistance of the peanut materials by using the compensatory growth rate.
[0011] In the above method, the peanut materials include, but are not limited to,
varieties, new strains, breeding materials, germplasm materials, etc.
[0012] In the above method, the key growth stages include, but are not limited to,
a seedling stage, a flowering and peg setting stage, a pod bearing stage or a full fruit
maturity stage.
[0013] In the above method, the drought treatment specifically comprises:
[0014] In the seedling stage, controlling the relative water content of soil to be
47%-53%; or
[0015] In the flowering and peg setting stage, controlling the relative water content
of soil to be 62%-68%; or
[0016] In the pod bearing stage, controlling the relative water content of soil to be
%-60%; or
[0017] In the full fruit maturity stage, controlling the relative water content of soil
to be 52%-58%.
[0018] In the above method, the rewatering refers to restoring normal water supply
and providing required water according to the growth stage of peanuts. Specifically:
[0019] T If peanuts are in the seedling stage during rewatering, the relative water
content of soil is controlled to be 55%-60%;
[0020] If peanuts are in the flowering and peg setting stage during rewatering, the
relative water content of soil is controlled to be 75%-80%;
[0021] If peanuts are in the pod bearing stage during rewatering, the relative water
content of soil is controlled to be 65%-75%; and
[0022] If peanuts are in the full fruit maturity stage during rewatering, the relative
water content of soil is controlled to be 60%-65%.
[0023] In the above method, the normal water supply of control treatment refers
to:
[0024] In the seedling stage, controlling the relative water content of soil to be
%-60%;
[0025] In the flowering and peg setting stage, controlling the relative water content
of soil to be 75%-80%;
[0026] In the pod bearing stage, controlling the relative water content of soil to be
%-75%; and
[0027] In the full fruit maturity stage, controlling the relative water content of soil
to be 60%-65%.
[0028] In the above method, the compensatory growth rate is calculated as
follows:
DWZ-DW1 W2-W1
[0029] compensatory growth rate (%)= Dwi xw100% x100
[0030] Wherein,
[0031] DW1 is the plant biomass at the end of drought treatment; DW2 is the plant
biomass 15 days after rewatering following drought treatment; W1 refers to the biomass of
control treatment at the time of DW1 sampling; and W2 is the biomass of control
treatment at the time of DW2 sampling.
[0032] The compensatory growth rate being greater than 0 indicates that the
peanut materials to be evaluated have a compensatory growth ability, and the larger the
number, the higher the compensatory growth ability. The compensatory growth rate being
less than 0 indicates that the peanut materials to be evaluated have no compensatory
growth ability.
[0033] The invention has the following beneficial effects:
[0034] According to the method, the compensatory growth ability of new peanut
varieties (strains) after drought stress is evaluated by conducting drought treatment and then rewatering, measuring the plant biomass data, and calculating the compensatory growth rate, so that the drought resistance of new peanut varieties (strains) can be quickly and accurately identified, and the efficiency and accuracy of drought-resistant peanut breeding are significantly improved.
DETAILED DESCRIPTION OF THE INVENTION
[0035] It should be noted that the following detailed description is exemplary and
is intended to provide a further explanation for the application. Unless otherwise specified,
the technical terms or scientific terms used in the application shall have the general
meaning understood by those of ordinary skill in the art to which the application belongs.
[0036] Term explanation:
[0037] Peanut seedling stage: from the time when 50% of the plants in the field
reach the seedling emergence standard to the time when 50% of the plants in the field first
bloom.
[0038] Peanut flowering and peg setting stage: from the time when 50% of the
plants in the field are in the peg setting state to the time when 50% of the plants have
cockscomb-shaped young fruits.
[0039] Peanut pod bearing stage: from the time when 50% of the plants in the field
have cockscomb-shaped young fruits to the time when 50% of the plants have the first full
fruit.
[0040] Full fruit maturity stage: from the time when 50% of plants have full fruits
to the time when most pods are full and mature.
[0041] Relative water content of soil = mass water content of soil + field moisture
capacity * 100%.
[0042] Mass water content of soil: the ratio of water mass in soil to dry soil mass.
Generally, the drying method is adopted for measurement.
[0043] Field moisture capacity: adequate water supply is ensured, surface covering
is conducted to avoid evaporation, measurement is conducted after water infiltration
reaches equilibrium, and the measured mass water content of soil is the field moisture
capacity.
[0044] As introduced in the background art, it is technically difficult to accurately
identify the drought resistance of new varieties (strains) in the process of breeding new
varieties, which is an important technical problem in drought-resistant peanut breeding.
[0045] In view of this, the invention provides a new method for evaluating the
drought resistance of peanuts by using a compensatory growth rate. The invention
conducts drought treatment and then rewatering treatment on new varieties (strains). There
are two kinds of treatment. The first kind of treatment is drought treatment followed by
rewatering treatment, that is, drought treatment is conducted at a certain key growth stage
of peanuts (which may be, but is not limited to, the seedling stage, the flowering and peg
setting stage, the pod bearing stage and the full fruit stage), and normal water supply is
restored at the end of this growth stage. The second kind of treatment is control treatment,
which provides normal water supply and suitable water according to peanut growth
demands. Sampling is conducted for plant biomass measurement at the end of drought
treatment and 15 days after rewatering, and the compensatory growth rate of peanuts is
calculated through the biomass before and after rewatering to evaluate the drought
resistance and yield potential of new varieties (strains).
[0046] In an embodiment of the invention, a method for identifying and evaluating
the drought resistance of peanuts by using a compensatory growth rate is provided, which
comprises the following steps:
[0047] Step 1: determining peanut varieties to be identified.
[0048] Step 2: peanut sowing. Peanuts were sowed at a suitable time. Two kinds of
treatment were conducted on each new variety. The first kind of treatment was drought treatment followed by rewatering treatment, and the second kind of treatment was control treatment which supplied suitable water. Each kind of treatment was repeated 3 times.
Ridge cultivation with plastic film mulching was conducted, with a ridge width of 85-90
cm, a ridge height of 10-15 cm, a small row spacing of not less than 35 cm, a hole spacing
of 15-20 cm, and 2 seeds per hole.
[0049] Step 3: in addition to the new varieties to be identified, adding two control
varieties Luhua 11 and 79266. Luhua 11 and 79266 can be used as standard varieties for
identification of strong and weak drought resistance of peanuts to facilitate the comparison
of the compensatory growth rate data of new varieties of different experimental groups,
different places and different years.
[0050] Step 4: timely conducting drought treatment and post-drought rewatering
treatment according to the drought treatment requirements during peanut growth, and
conducting control treatment. Remember to keep out the rain.
[0051] The drought treatment specifically comprises:
[0052] In the seedling stage, controlling the relative water content of soil to be
47%-53%; or
[0053] In the flowering and peg setting stage, controlling the relative water content
of soil to be 62%-68%; or
[0054] In the pod bearing stage, controlling the relative water content of soil to be
%-60%; or
[0055] In the full fruit maturity stage, controlling the relative water content of soil
to be 52%-58%.
[0056] The rewatering refers to restoring normal water supply and providing
required water according to the growth stage of peanuts. Specifically:
[0057] If peanuts are in the seedling stage during rewatering, the relative water
content of soil is controlled to be 55%-60%;
[0058] If peanuts are in the flowering and peg setting stage during rewatering, the
relative water content of soil is controlled to be 75%-80%;
[0059] If peanuts are in the pod bearing stage during rewatering, the relative water
content of soil is controlled to be 65%-75%; and
[0060] If peanuts are in the full fruit maturity stage during rewatering, the relative
water content of soil is controlled to be 60%-65%.
[0061] The control treatment refers to:
[0062] In the seedling stage, controlling the relative water content of soil to be
%-60%;
[0063] In the flowering and peg setting stage, controlling the relative water content
of soil to be 75%-80%;
[0064] In the pod bearing stage, controlling the relative water content of soil to be
%-75%; and
[0065] In the full fruit maturity stage, controlling the relative water content of soil
to be 60%-65%.
[0066] Step 5: conducting sampling for plant biomass measurement at the end of
drought treatment and 15 days after rewatering, and conducting sampling for control
treatment too at the same stage. 10 plants were sampled from each area for each kind of
treatment. The whole plant was put in an oven for half an hour for fixation at 105°C, then
the temperature was adjusted to 80°C, and then drying and weighing were conducted.
[0067] Step 6: calculating the compensatory growth rate.
DW2-DW1 X 100% - X 100%
[0068] Compensatory growth rate (%)= Dwi w 1
[0069] DW1 is the plant biomass at the end of drought treatment; DW2 is the plant
biomass 15 days after rewatering following drought treatment; W1 refers to the biomass of
control treatment at the time of DW1 sampling; and W2 is the biomass of control
treatment at the time of DW2 sampling.
[0070] The compensatory growth rate being greater than 0 indicates that the new
strains have a compensatory growth ability, and the larger the number, the higher the
compensatory growth ability. The compensatory growth rate being less than 0 indicates
that the new strains have no compensatory growth ability.
[0071] The indicator of the compensatory growth rate proposed by the invention is
more in line with the actual production situation, and can better describe the drought
resistance of peanuts and the potential of resuming growth after drought. Peanuts
generally suffer from drought at a certain stage of growth, and will show compensatory
growth after getting water supply from irrigation or rain. The compensatory growth rate is
the evaluation of this compensatory growth ability of varieties. The method of the
invention can quickly and accurately identify the drought resistance of new peanut
varieties (strains), and significantly improve the efficiency and accuracy of
drought-resistant peanut breeding.
[0072] In order to enable those skilled in the art to understand the technical
solution of this application more clearly, the technical solution of this application will be
described in detail with specific embodiments below.
[0073] Embodiment 1: Evaluate drought resistance of Luhua 11 and 79266 at
seedling stage by compensatory growth rate
[0074] (1) Peanut sowing. Peanuts were sowed at a suitable time. Two kinds of
treatment were conducted on each variety. The first kind of treatment was drought
treatment followed by rewatering treatment, and the second kind of treatment was control
treatment which supplied suitable water. Each kind of treatment was repeated 3 times.
Ridge cultivation with plastic film mulching was conducted, with a ridge width of 85-90
cm, a ridge height of 10-15 cm, a small row spacing of not less than 35 cm, a hole spacing
of 15-20 cm, and 2 seeds per hole.
[0075] (2) For the first kind of treatment, conducting drought treatment in the whole seedling stage of peanuts, controlling the relative water content of soil to be
47%-53%, and after the drought treatment, conducting rewatering and controlling the
relative water content of soil to be 75%-80%; and for the second kind of treatment which
is control treatment, controlling the relative water content of soil to be 55%-60% in the
whole seedling stage, and controlling the relative water content of soil to be 75%-80% in
the rewatering treatment stage corresponding to the first kind of treatment.
[0076] Sampling was conducted for plant biomass measurement at the end of
drought treatment and 15 days after rewatering, and sampling was conducted for control
treatment too at the same stage. 10 plants were sampled from each area for each kind of
treatment. The whole plant was put in an oven for half an hour for fixation at 105°C, then
the temperature was adjusted to 80°C, and then drying and weighing were conducted.
[0077] (3) Calculating the compensatory growth rate:
DWZ-DWl X 100% - X 100%
[0078] Compensatory growth rate (%)= DWi. w 1
[0079] DW1 is the plant biomass at the end of drought treatment; DW2 is the plant
biomass 15 days after rewatering following drought treatment; W1 refers to the biomass of
control treatment at the time of DW1 sampling; and W2 is the biomass of control
treatment at the time of DW2 sampling.
[0080] The compensatory growth rate being greater than 0 indicates that the new
strains have a compensatory growth ability, and the larger the number, the higher the
compensatory growth ability. The compensatory growth rate being less than 0 indicates
that the new strains have no compensatory growth ability.
[0081] According to calculation results, the compensatory growth rate of Luhua 11
at the seedling stage was 0.26, and the compensatory growth rate of 79266 at the seedling
stage was 0.04.
[0082] Embodiment 2: Evaluate drought resistance of Luhua 11 and 79266 at
flowering and peg setting stage by compensatory growth rate
[0083] (1) Peanut sowing. Peanuts were sowed at a suitable time. Two kinds of
treatment were conducted on each variety. The first kind of treatment was drought
treatment followed by rewatering treatment, and the second kind of treatment was control
treatment which supplied suitable water. Each kind of treatment was repeated 3 times.
Ridge cultivation with plastic film mulching was conducted, with a ridge width of 85-90
cm, a ridge height of 10-15 cm, a small row spacing of not less than 35 cm, a hole spacing
of 15-20 cm, and 2 seeds per hole.
[0084] (2) For the first kind of treatment, conducting drought treatment in the
whole flowering and peg setting stage of peanuts, controlling the relative water content of
soil to be 62%-68%, and after the drought treatment, conducting rewatering and
controlling the relative water content of soil to be 65%-75%; and for the second kind of
treatment which is control treatment, controlling the relative water content of soil to be
%-80% in the whole flowering and peg setting stage, and controlling the relative water
content of soil to be 65%-75% in the rewatering treatment stage corresponding to the first
kind of treatment.
[0085] Sampling was conducted for plant biomass measurement at the end of
drought treatment and 15 days after rewatering, and sampling was conducted for control
treatment too at the same stage. 10 plants were sampled from each area for each kind of
treatment. The whole plant was put in an oven for half an hour for fixation at 105°C, then
the temperature was adjusted to 80°C, and then drying and weighing were conducted.
[0086] (3) Calculating the compensatory growth rate:
[0087] The compensatory growth rate was calculated in the same way as in
Embodiment 1. According to calculation results, the compensatory growth rate of Luhua
11 at the flowering and peg setting stage was 0.23, and the compensatory growth rate of
79266 at the flowering and peg setting stage was 0.03.
[0088] Luhua 11 is a known peanut variety with strong drought resistance and
79266 a peanut variety with weak drought resistance. By using Luhua 11 and 79266 as
control varieties to verify the peanut drought resistance evaluation method of the invention,
it was shown that the identification results of this method were consistent with the drought
resistance of standard varieties. Therefore, the method of the invention can be used for
evaluating the drought resistance of peanuts.
[0089] The above embodiments are only preferred ones of this application, and are
not used to limit this application. For those skilled in the art, this application can be
modified and varied. Any modification, equivalent substitution, improvement, etc. made
within the spirit and principle of this application shall be included in the protection scope
of this application.

Claims (6)

What is claimed is:
1. A method for identifying and evaluating the drought resistance of peanuts by using a
compensatory growth rate, characterized by comprising the following steps:
conducting drought treatment on peanut materials to be evaluated in key growth stages
and rewatering after drought treatment; and taking normal water supply as a control,
calculating the compensatory growth rate by measuring plant biomass at the end of
drought treatment and 15 days after rewatering, and evaluating the drought resistance of
the peanut materials by using the compensatory growth rate.
2. The method according to claim 1, characterized in that the key growth stages
include a seedling stage, a flowering and peg setting stage, a pod bearing stage or a full
fruit maturity stage.
3. The method according to claim 1 or 2, characterized in that the drought treatment
specifically comprises:
in the seedling stage, controlling the relative water content of soil to be 47%-53%; or,
in the flowering and peg setting stage, controlling the relative water content of soil to
be 62%-68%; or,
in the pod bearing stage, controlling the relative water content of soil to be 55%-60%;
or,
in the full fruit maturity stage, controlling the relative water content of soil to be
52%-58%.
4. The method according to claim 1 or 2, characterized in that the rewatering refers to
restoring normal water supply and providing required water according to the growth stage
of peanuts; specifically:
if peanuts are in the seedling stage during rewatering, the relative water content of soil
is controlled to be 55%-60%;
if peanuts are in the flowering and peg setting stage during rewatering, the relative water content of soil is controlled to be 75%-80%; if peanuts are in the pod bearing stage during rewatering, the relative water content of soil is controlled to be 65%-75%; and if peanuts are in the full fruit maturity stage during rewatering, the relative water content of soil is controlled to be 60%-65%.
5. The method according to claim 1 or 2, characterized in that the normal water supply
of control treatment refers to:
in the seedling stage, controlling the relative water content of soil to be 55%-60%;
in the flowering and peg setting stage, controlling the relative water content of soil to
be 75%-80%;
in the pod bearing stage, controlling the relative water content of soil to be 65%-75%;
and
in the full fruit maturity stage, controlling the relative water content of soil to be
%-65%.
6. The method according to any one of claims 1-5, characterized in that the
compensatory growth rate is calculated as follows:
DW2-DW1 X-W X 100% compensatory growth rate (%)= DWi Wi
wherein,
DW1 is the plant biomass at the end of drought treatment; DW2 is the plant biomass
days after rewatering following drought treatment; WI refers to the biomass of control
treatment at the time of DW1 sampling; and W2 is the biomass of control treatment at the
time of DW2 sampling.
AU2021104130A 2021-06-28 2021-07-14 Method for identifying and evaluating drought resistance of peanuts by using compensatory growth rate Ceased AU2021104130A4 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112673918A (en) * 2021-01-21 2021-04-20 上海市农业科学院 Method for identifying waterlogging tolerance of vegetable soybeans

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116542545A (en) * 2023-03-20 2023-08-04 中国水利水电科学研究院 Summer corn comprehensive recovery power evaluation method under drought condition

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112673918A (en) * 2021-01-21 2021-04-20 上海市农业科学院 Method for identifying waterlogging tolerance of vegetable soybeans

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