CN108934814A - A method of identification cabbage heart heat resistance - Google Patents
A method of identification cabbage heart heat resistance Download PDFInfo
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- CN108934814A CN108934814A CN201810860003.0A CN201810860003A CN108934814A CN 108934814 A CN108934814 A CN 108934814A CN 201810860003 A CN201810860003 A CN 201810860003A CN 108934814 A CN108934814 A CN 108934814A
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- 240000007124 Brassica oleracea Species 0.000 title claims abstract description 73
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 title claims abstract description 73
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 title claims abstract description 73
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 16
- 241001290610 Abildgaardia Species 0.000 claims abstract description 24
- 238000010899 nucleation Methods 0.000 claims abstract description 19
- 241000196324 Embryophyta Species 0.000 claims abstract description 14
- 238000011161 development Methods 0.000 claims abstract description 8
- 238000011156 evaluation Methods 0.000 claims abstract description 3
- 235000019082 Osmanthus Nutrition 0.000 claims description 34
- 241000333181 Osmanthus Species 0.000 claims description 34
- 241000124033 Salix Species 0.000 claims description 16
- 238000009331 sowing Methods 0.000 claims description 11
- 235000000981 Brassica parachinensis Nutrition 0.000 claims description 5
- 241001604868 Brassica rapa var. parachinensis Species 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 3
- 239000013558 reference substance Substances 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims 1
- 235000013311 vegetables Nutrition 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 3
- 235000016068 Berberis vulgaris Nutrition 0.000 description 17
- 241000335053 Beta vulgaris Species 0.000 description 17
- 230000018109 developmental process Effects 0.000 description 6
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 2
- 206010019345 Heat stroke Diseases 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 230000009418 agronomic effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 230000001932 seasonal effect Effects 0.000 description 2
- 241000219198 Brassica Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000510678 Falcaria vulgaris Species 0.000 description 1
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of methods for identifying cabbage heart heat resistance, belong to Heat Resistance in Vegetables research field.Different dates of seeding is arranged from 11~December of current year to April in the coming year by the present invention, and interval date of seeding sows kind to be measured and check variety；High Temperature Stress environment is arranged in the cabbage heart of identical date of seeding；The cabbage heart of each date of seeding is respectively using the plant height of neat mouth phase cabbage heart, development degree, the number of blade, main a kind of sedge height and main a kind of sedge diameter as Themotalerance, using percentage of blooming as the heat resistance of heat-resistance index evaluation cabbage heart；The check variety includes the early-maturing variety and late variety of known heat resistance；When the percentage of blooming of the kind to be measured is similar to the early-maturing variety of known heat resistance, then heat resistance is strong；When the percentage of blooming of the kind to be measured is similar to the late variety of known heat resistance, then heat resistance is weak.Simple and practical, strong operability of the invention, working strength is low, reduces repetition, reduces workload.
Description
Technical field
The invention belongs to Heat Resistance in Vegetables research fields, are specifically related to a kind of method for identifying cabbage heart heat resistance.
Background technique
Cabbage heart also known as tender flower stalk (flowering chinese cabbage) are that Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage is sub-
To spend the mutation for being product in kind.It is known as " after in dish " and " hats of vegetable product ", is that South China of China cultivated area is maximum
One of vegetable crop, also have larger cultivated area elsewhere in China in recent years.But the high temperature stress in season at the beginning of the autumn in midsummer,
Normal growth and the development for having seriously affected cabbage heart cause its yield and quality to decline.Awareness and understanding high temperature stress is to vegetables
The influence of economical character finds characteristic index relevant to heat resistance, facilitates the heat resistance for taking measures to improve vegetables, and can
Screening for high temperature resistant kind provides evaluation index.But the research work master in terms of cabbage heart heat resistance both at home and abroad so far
It concentrates on simple economical character and physiological character, the index of economical character has plant height (PH), development degree (Width) base
The number of sheets (BLN), a kind of sedge number of sheets (SSLN), length of blade (LL), petiole long (PL), leaf width (LW), petiole wide (PW), leaf thick (LT),
A kind of sedge high (SSH), a kind of sedge thick (SSD), a kind of sedge weight (SSW), gross weight (TW) and sowing survival rate (SR) etc., physiological and biochemical index has malonaldehyde
(MDA), proline (Pro), conductivity (ec), chlorophyll (Ch) etc..The standard method of general heat resistance is to utilize high temperature canopy room
Identify different ecological profile material, all very stringent to facility and operating condition, the identification work under hot conditions is also arduouser, holds
The fortuitous events such as heatstroke easily occur, and qualification result obtained in high temperature canopy room not necessarily meets the high temperature of practical plantation situation
Adverse circumstance injures result.There is presently no the reports that one kind can simply, directly measure cabbage heart heat resistance.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of method for identifying a large amount of cabbage heart heat resistances, it can be simple, straight
The heat resistance of ground connection quantitative determination cabbage heart.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of methods for identifying cabbage heart heat resistance, comprising the following steps:
Different dates of seeding, is set from 11~December of current year to April in the coming year, sows kind to be measured and right in each date of seeding respectively
According to kind, the check variety includes heat-resisting early-maturing variety and heat labile late variety；Temperature is arranged in the cabbage heart of different sowing dates
Difference ring border；The cabbage heart of each date of seeding is respectively with the plant height of neat mouth phase cabbage heart, development degree, the number of blade, main a kind of sedge height and main a kind of sedge diameter
For Themotalerance, the heat resistance of cabbage heart is evaluated to bloom percentage as heat-resistance index；When the kind to be measured Themotalerance with
Heat-resisting early-maturing variety is similar, then heat resistance is strong；When the Themotalerance of the kind to be measured is similar to heat labile late variety,
Then heat resistance is weak；When the percentage of blooming of the kind to be measured is similar to heat-resisting early-maturing variety, then heat resistance is strong；When it is described to
The percentage of blooming for surveying kind is similar to heat labile late variety, then heat resistance is weak.
Preferably, the time interval of the date of seeding is independently 7 days, 10 days, 15 days, 21 days or 30 days.
Preferably, point plantation such as the kind to be measured and check variety, the equal part plantation is to plant on homalographic soil
Plant the Brassica parachinensis seeds of equivalent.
Preferably, the known heat-resisting early-maturing variety includes four or nine cabbage hearts.
Preferably, the known heat labile late variety includes osmanthus willow evening in October cabbage heart.
The present invention provides a kind of methods for identifying cabbage heart heat resistance, utilize the different sowing dates interval from winter to summer, manufacture
Natural high-temperature adverse circumstance environment from low to high, saves high temperature chamber facility expense.Using natural temperature variation as Heat tolerance identification
Condition works in a natural environment, avoids high temperature greenhouse and identifies that the heatstroke problem occurred, working strength reduce.With the neat mouth phase
Based on breeding time, cabbage heart Heat tolerance identification technology supplemented by 5 main agronomic phenotypes trait phenotypes is simple and practical and can operate
Property it is strong, save the expense of the Physiology and biochemistry identification during cabbage heart Heat tolerance identification, reached preliminary understanding and identification cabbage heart
The purpose of germ plasm resource heat resistance, reduces repetition, reduces workload, has saved time and property.
Specific embodiment
The present invention provides a kind of methods for identifying cabbage heart heat resistance, comprising the following steps:
Different dates of seeding, is set from 11~December of current year to April in the coming year, sows kind to be measured and right in each date of seeding respectively
According to kind, the check variety includes heat-resisting early-maturing variety and heat labile late variety；Temperature is arranged in the cabbage heart of different sowing dates
Difference ring border；The cabbage heart of each date of seeding is respectively with the plant height of neat mouth phase cabbage heart, development degree, the number of blade, main a kind of sedge height and main a kind of sedge diameter
For Themotalerance, the heat resistance of cabbage heart is evaluated to bloom percentage as heat-resistance index；When the kind to be measured Themotalerance with
Heat-resisting early-maturing variety is similar, then heat resistance is strong；When the Themotalerance of the kind to be measured is similar to heat labile late variety,
Then heat resistance is weak；When the percentage of blooming of the kind to be measured is similar to heat-resisting early-maturing variety, then heat resistance is strong；When it is described to
The percentage of blooming for surveying kind is similar to heat labile late variety, then heat resistance is weak.
Different dates of seeding is arranged from 11~December of current year to April in the coming year by the present invention, and kind to be measured and reference substance are sowed in interval
Kind, the interval of the date of seeding is preferably independently 7 days, 10 days, 15 days, 21 days or 30 days, and more preferably 10 days or 15 days, most
Preferably 10 days.
In the present invention, the kind to be measured and the preferred equal part plantation of check variety, the equal part plantation preferably exist
The Brassica parachinensis seeds of equivalent are planted on homalographic soil.Check variety of the present invention preferably includes known heat-resisting early-maturing variety
With known heat labile late variety, the known heat-resisting early-maturing variety includes four or nine cabbage hearts, and four or nine cabbage hearts are early seasonal strain, resistance to
It is hot strong, it can be sowed in spring, summer, early autumn, cold resistance is not strong, sows in autumn and winter and is easy early bolting, causes the underproduction；Institute
Stating known heat labile late variety includes osmanthus willow evening in October cabbage heart, and osmanthus willow evening in October is seasonal strain, and heat resistance is not strong, in summer
Sowing only long leaf not bolting, cold resistance is strong, sows in osmanthus backlands area autumn and winter, well-grown；It is preferably divided when plantation
The cell of area, the area of the cell are preferably 2~20 square metres, more preferably 5~15 square metres, most preferably
10 square metres；Plant the Brassica parachinensis seeds of identical weight respectively in each cell area, the plantation amount of the Brassica parachinensis seeds is preferred
80~500, more preferably 100~300, most preferably 200.
There is no any special restrictions to the management after the cabbage heart plantation of different sowing dates by the present invention, utilize the normal of this field
Rule means are managed maintenance.
Temperature difference environment is arranged using the cabbage heart of different sowing dates in the present invention, and the cabbage heart of each date of seeding is respectively with neat mouth phase cabbage heart
Plant height, development degree, the number of blade, main a kind of sedge height and main a kind of sedge diameter are Themotalerance, evaluate dish as heat-resistance index to bloom percentage
The heat resistance of the heart.In the present invention, due to being sowed from 11~December of current year to interval in April in the coming year, so there are a temperature
High Temperature Stress environment from low to high is related in the neat mouth phase of different sowing dates and its phenotype by the late-maturing cabbage heart of different heat resistances
Character variation, to evaluate the heat resistance of cabbage heart, the neat mouth phase preferably refers to the top of cabbage heart and the neat high number of days of bud, institute
State percentage of blooming preferably refer to the neat mouth phase bloom plant account for this plantation cell with kind plantation plant sum percentage.At this
In invention, the quality of plant strain growth can be tentatively judged according to plant phenotype correlated traits, heat labile bolting very early, carefully
If chopsticks item does not have yield, and it is heat-resisting can also grow stem's thickening, so being referring initially to preceding supplemented by Themotalerance in the present invention
Phase appearance tentatively judges heat resistance；Then here mouth phase bolting is bloomed carries out the rational judgment of heat resistance again.In this hair
In bright, when the percentage of blooming of the kind to be measured is similar to known heat-resisting early-maturing variety, then heat resistance is strong；When described to be measured
The percentage of blooming of kind is similar to known heat labile late variety, then heat resistance is weak；Preferably, the heat resistance is divided
For four grades, respectively heat resistant type, general heat resistant type, general thermosensitive type, thermosensitive type, the heat resistant type is preferably product to be tested
The percentage of blooming of kind is 0~25%；The percentage of blooming that the general heat resistant type is preferably kind to be measured is 26~50%；
The percentage of blooming that the general thermosensitive type is preferably kind to be measured is 51~75%；The thermosensitive type is preferably product to be tested
The percentage of blooming of kind is 76~100%.
Identification cabbage heart heat resistance method provided by the invention is described in detail below with reference to embodiment, but cannot
They are interpreted as limiting the scope of the present invention.
Embodiment 1
Kind to be measured: the osmanthus beet heart No. 1 and No. 2；
Check variety: four or nine heat-resisting cabbage hearts are chosen as heat-resisting control, heat labile osmanthus willow evening in October cabbage heart is chosen and makees
For thermo-labile control；
Sowing time: on December 5th, 2016 be initial sowing time, by the phase arrange on December 17th, 2016, on January 5th, 2017,
On January 11st, 2017, on January 15th, 2017, on 2 8th, 2017, on January 25th, 2017,6 days 2 months, 2 months 2017 2017
15 days, on March 8th, 2017, on March 18th, 2017, on April 27th, 2017 sow kind to be measured and check variety.
The percentage of blooming for measuring each date of seeding neat mouth phase respectively, averages, and measures heat resistance.The results are shown in Table 1:
The Themotalerance of table a kind to be measured and check variety
It can be seen from the above, the neat mouth phase: identical, osmanthus willow evening in October cabbage heart and the osmanthus of precocious four or nine cabbage hearts and the osmanthus beet heart 2
The beet heart 1 neat mouth phase postpones, and osmanthus willow evening in October cabbage heart is postponed most.
Plant height: precocious four or nine cabbage hearts and the osmanthus beet heart 2 it is lower, osmanthus willow evening in October cabbage heart and the osmanthus beet heart 1
Increase, osmanthus willow evening in October cabbage heart highest.
Development degree: precocious four or nine cabbage hearts and the osmanthus beet heart 2 it is smaller, osmanthus willow evening in October cabbage heart and the osmanthus beet heart 1
Noticeably greater than precocious four or nine cabbage hearts and the osmanthus beet heart 2, osmanthus willow evening in October cabbage heart is maximum.
The number of blade: the number of blade of precocious four or nine cabbage hearts and the osmanthus beet heart 2 is less, osmanthus willow evening in October cabbage heart and the osmanthus beet heart
No. 1 more, osmanthus willow evening in October cabbage heart it is most.
Main a kind of sedge height and main a kind of sedge diameter: most important commodity organ, good main a kind of sedge are the important indicators of yield.Precocity four
Lower, relatively fine, the highest of osmanthus willow evening in October cabbage heart of nine cabbage hearts and the osmanthus beet heart 2.Four or nine cabbage hearts are typical heat-resisting product
Kind, main a kind of sedge height is lower, also most very thin.Osmanthus willow evening in October cabbage heart is Guangxi Local kind, and main side a kind of sedge is taken in concurrently, single a kind of sedge diameter compared with
It is very thin, and the osmanthus beet heart 1 is medium variety, using main a kind of sedge as yield organ, main a kind of sedge diameter is apparently higher than other kinds, meets it
Breediness.The osmanthus beet heart 2 is early-maturing variety, and main a kind of sedge height is minimum, and a kind of sedge stem is minimum.
Bloom percentage (%): precocious four or nine cabbage hearts and the osmanthus beet heart 2 are significantly higher than osmanthus willow evening in October cabbage heart and osmanthus
The beet heart 1, show that the kind osmanthus beet heart 2 heat resistances to be measured are higher, the heat resistance of the osmanthus beet heart 1 is poor.
As seen from the above embodiment, cabbage heart Heat tolerance identification method through the invention, obtained qualification result from it is different
The practical heat resistance of environmental cabbage heart is consistent, based on neat breeding time mouth phase, supplemented by 5 main agronomic phenotypes trait phenotypes
Cabbage heart Heat tolerance identification technology, simple and practical and strong operability, the physiology saved during cabbage heart Heat tolerance identification is raw
The expense for changing identification has achieved the purpose that preliminary understanding and identification cabbage heart germ plasm resource heat resistance, and accuracy rate is high, reduces weight
It is multiple, workload is reduced, time and property have been saved.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (5)
1. a kind of method for identifying cabbage heart heat resistance, comprising the following steps:
Different dates of seeding, is set from 11~December of current year to April in the coming year, sows kind to be measured and reference substance respectively in each date of seeding
Kind, the check variety includes heat-resisting early-maturing variety and heat labile late variety；Temperature difference ring is arranged in the cabbage heart of different sowing dates
Border；The cabbage heart of each date of seeding is respectively resistance to the plant height of neat mouth phase cabbage heart, development degree, the number of blade, main a kind of sedge height and main a kind of sedge diameter
Heating index, using percentage of blooming as the heat resistance of heat-resistance index evaluation cabbage heart；When the kind to be measured Themotalerance with it is heat-resisting
Early-maturing variety it is similar, then heat resistance is strong；It is when the Themotalerance of the kind to be measured is similar to heat labile late variety, then resistance to
It is hot weak；When the percentage of blooming of the kind to be measured is similar to heat-resisting early-maturing variety, then heat resistance is strong；When the product to be tested
The percentage of blooming of kind is similar to heat labile late variety, then heat resistance is weak.
2. the method according to claim 1, wherein the time interval of the date of seeding is independently 7 days, 10 days,
15 days, 21 days or 30 days.
3. the method according to claim 1, wherein point plantation such as the kind to be measured and check variety, described
Equal part plantation is the Brassica parachinensis seeds that equivalent is planted on homalographic soil.
4. the method according to claim 1, wherein the heat-resisting early-maturing variety includes four or nine cabbage hearts.
5. the method according to claim 1, wherein the heat labile late variety includes osmanthus willow evening in October dish
The heart.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810860003.0A CN108934814B (en) | 2018-08-01 | 2018-08-01 | Method for identifying heat resistance of cabbage heart |
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| CN201810860003.0A CN108934814B (en) | 2018-08-01 | 2018-08-01 | Method for identifying heat resistance of cabbage heart |
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| CN108934814B CN108934814B (en) | 2020-07-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113348992A (en) * | 2021-06-08 | 2021-09-07 | 海南省农业科学院蔬菜研究所 | Evaluation method for heat resistance of cabbage |
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| CN1804584A (en) * | 2006-01-17 | 2006-07-19 | 南京大学 | Method for quickly identifying heat tolerance of lawn type tall fescue |
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| YANG, L.等: "The research on identification technology of heat tolerance of Chinese cabbage (Brassica campestris L., ssp. pekinensis).", 《ACTA HORTICULTURAE》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113348992A (en) * | 2021-06-08 | 2021-09-07 | 海南省农业科学院蔬菜研究所 | Evaluation method for heat resistance of cabbage |
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