CN113063903A - Adjustable isotope labeling device for plant photosynthesis - Google Patents
Adjustable isotope labeling device for plant photosynthesis Download PDFInfo
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- CN113063903A CN113063903A CN202110450340.4A CN202110450340A CN113063903A CN 113063903 A CN113063903 A CN 113063903A CN 202110450340 A CN202110450340 A CN 202110450340A CN 113063903 A CN113063903 A CN 113063903A
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- 230000029553 photosynthesis Effects 0.000 title claims abstract description 44
- 238000010672 photosynthesis Methods 0.000 title claims abstract description 44
- 238000001948 isotopic labelling Methods 0.000 title claims abstract description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 172
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 91
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- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/004—CO or CO2
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Abstract
The invention provides an adjustable isotope labeling device for plant photosynthesis, which comprises: organic glass 1 and sealing washer 2 constitute external circulation leaf room 3, external circulation leaf room 3 communicates with carbon dioxide absorption cell 11 through organic hose 13, external circulation leaf room 3 communicates with circulating pump 4 through organic hose 5, external circulation leaf room 3 is fixed on multi-angle mount 6 through metal branch 7, carbon dioxide concentration test probe 8 is put into in external circulation leaf room 3, carbon dioxide concentration test probe 8 is linked together with carbon dioxide detector 10 through optic fibre 9, carbon dioxide absorption cell 11 communicates with circulating pump 4 through organic hose, carbon dioxide supply pipe 12 communicates with organic hose 5 through tee bend electronic control valve, the carbon dioxide is supplied with to electronic control carbon dioxide velocity of flow assurance, carbon dioxide holding vessel 15 communicates with tee bend electronic control valve, organic hose 5 through carbon dioxide supply pipe 12.
Description
Technical Field
Belongs to the technical field of plant photosynthesis detection, and particularly relates to an adjustable isotope labeling device for plant photosynthesis.
Background
At present, Licor-6800, LCi-SD, CIRAS-3 and other portable photosynthesis instruments are available in the market, and provide a photosynthesis determination technical means for plant ecological research. This kind of photosynthetic apparatus comparison is applicable to the indoor control experiment, however, when the field experiment, because the admission line that is furnished with is not isolated with the external world, easily receive the interference of ground human breathing and ground activity to cause the inhomogeneous problem of business turn over gas, the different accuracy that directly influences plant photosynthesis of the different angles of growth of the locellus undersize of survey plant leaf and plant leaf in addition.
Chinese patent application 201310682081.3, the title of the invention "Whole plant tissue culture seedling photosynthetic rate rapid determination device" discloses a whole plant tissue culture seedling photosynthetic rate rapid determination device and method, including the IRGA sample room of the plant photosynthetic apparatus, the mounting plate of the leaf room of the soil, RGA sample room and mounting plate of the leaf room of the soil make up the complex, there is a transparent homemade leaf room in addition, there are fixed orifices, air vents and backflow union on the homemade leaf room; the self-made leaf chamber is connected with the soil leaf chamber mounting plate through a fixing hole, and the self-made leaf chamber is in rigid sealing connection with the complex; the RGA sample chamber is connected with the photosynthetic apparatus host computer through a data line. Although the invention improves the prior plant photosynthetic determinator, adopts the self-made leaf chamber to replace the standard leaf chamber, the photosynthetic apparatus can intuitively display the photosynthetic rate of the plant under the condition of no mechanical compression damage in real time, and can randomly determine the photosynthetic rate of a single plant or a whole bottle (group) of tissue culture seedlings; also solves the problem that the area is less than 6cm in the standard leaf chamber2The problem of single leaf, but the photosynthesis of single leaf of a certain plant cannot be measured.
Chinese patent application 201520814463.1, the title of the invention "a plant photosynthetic rate measuring device" discloses a plant photosynthetic rate measuring device, which comprises an LED lamp, a light source for providing plant light, and a light intensity adjusting device; air pump for delivering CO2A gas; a vane chamber for fixing the measuring vane; flow meter, detecting CO2The gas flow rate; CO 22Analyzer, detecting CO2Concentration; temperature and humidity sensor for detecting CO2The temperature and humidity of the gas change; the data acquisition unit is used for collecting and recording the acquired data; and the computer analyzes the collected data. But not control CO2Concentration and photosynthetic detection of leaves in different directions.
The Chinese patent application 201610961666.2 entitled "photosynthetic determination error correction method based on physiological model under field conditions" discloses a measurement recordPhotosynthetic value A and atmospheric water vapor pressure difference DsTemperature value TK(ii) a Then respectively measuring ambient condition value atmospheric water vapor pressure difference value D in the lights standardTemperature value TK standardDifference value D from the atmospheric water vapor pressure value under the standard environmental condition to be calibrateds standardTemperature value TK standardRespectively substituting into the correction formula to obtain ADs,TKAnd with obtaining ADs Standard, TK StandardThen corrected to obtain ACalibration. The method can quantify the influence of environmental factors (such as temperature and humidity) on photosynthesis through the photosynthetic physiological model, thereby carrying out corresponding correction according to the change values of the temperature and the humidity in the photosynthetic determination process.
Chinese patent application 201721770487.7 entitled "device for measuring photosynthetic rate, respiration rate and greenhouse gas emission at different heights of crop population" discloses a device comprising a test box, a first support rod, a second support rod, a third support rod, 3N detection components and a signal collector, wherein the test box comprises a top cover, box and base, be equipped with first recess on the box top, the box wall mounting fan in, a pair of support frame is installed to the symmetry on the box inner wall, first branch, second branch, the both ends of third branch are fixed on two support frames through the detachably mode respectively, first branch, second branch, equidistant distribution on vertical direction of third branch, first branch, install a N determine module on second branch and the third branch respectively, the second recess that the base top was equipped with, each electrical components is connected with the signal collector electricity respectively. The method is based on the device, can measure the light intensity, the temperature and humidity, the photosynthetic rate, the respiratory rate and the emission of greenhouse gases at different heights of a crop group, but cannot measure the leaves of plants in different directions.
Chinese patent application 201711446350.0 entitled "device for extending temperature control range of photosynthesis determination system by circulating alcohol" discloses a device for controlling temperature range, which utilizes a circulating pump to deliver cooled or heated liquid alcohol, and sends the liquid alcohol to a metal part (containing a plurality of water channels for alcohol to flow through) tightly attached to an LI-6400XT photosynthesis measurement chamber through a PVC pipe, and changes the temperature of the metal part; the temperature of the photosynthesis measuring chamber is gradually adjusted through the heat exchange process, and finally, the temperature control range of the portable photosynthesis measuring system (LI-6400XT) is effectively expanded. The device expands the range of the temperature of the leaf chamber regulated and controlled by the LI-6400XT portable photosynthetic measurement system from the original environment temperature of +/-7 ℃ to 5-40 ℃, but the measured leaves are limited.
Chinese patent application 201720515458.X, the title of the invention, "photosynthetic assay air inlet auxiliary system for uniform dust fall" discloses a photosynthetic assay air inlet auxiliary system for uniform dust fall, which comprises an air inlet filtration homogenizing device and an air inlet connecting pipe, wherein the air inlet filtration homogenizing device comprises a hanging rope, a filter membrane fixing cover, an air inlet buffering barrel and an air inlet pipe connecting cover, a first external thread is arranged on the top side of the outer side wall of the air inlet buffering barrel, a second external thread is arranged on the bottom side of the outer side wall of the air inlet buffering barrel, a first internal thread is arranged on the inner side wall of the filter membrane fixing cover, the filter membrane fixing cover is connected with the top of the air inlet buffering barrel, two hanging rope holes are symmetrically arranged on the top side of the filter membrane fixing cover, the filter membrane fixing cover is fixedly connected with the hanging rope, the filter membrane is mutually extruded and fixed with the air inlet buffering barrel through the filter membrane fixing, the air inlet pipe connecting cover is fixedly connected with the bottom of the air inlet buffering barrel, and one end of the air inlet connecting pipe penetrates through the air inlet pipe connecting hole to be clamped with the rubber ring. Although the area of the leaf chamber is increased, the space of the leaf chamber is large, air can not circulate, and the photosynthesis measurement is inaccurate; the self-made leaf chamber is fixed through the hanging rope, the blades at different angles cannot be measured, the size of the leaf chamber cannot be adjusted along with the size of the blades, and the self-made leaf chamber is not suitable for high-precision measurement of a single blade.
Chinese patent application 201711446350.0 entitled "device for extending temperature control range of photosynthesis determination system by circulating alcohol" discloses a device for extending temperature control range of photosynthesis determination system by circulating alcohol, which utilizes circulating pump to deliver cooled or heated liquid alcohol, and sends the liquid alcohol to a metal part (containing a plurality of water channels for flowing alcohol inside) tightly attached to LI-6400XT photosynthesis measurement chamber by PVC pipe, and changes the temperature of the metal part; the temperature of the photosynthesis measuring chamber is gradually adjusted through the heat exchange process, and finally, the temperature control range of the portable photosynthesis measuring system (LI-6400XT) is effectively expanded. The device expands the range of regulating and controlling the leaf chamber temperature of the LI-6400XT portable photosynthesis determination system from the original environment temperature of +/-7 ℃ to 5-40 ℃, so that the photosynthesis parameters of the plants/crops in the field determination are not limited by the external environment temperature, and a measurement technology and a measurement method are provided for deeply discussing the response and adaptability mechanism of the physiological and ecological processes of the plants/crops to the temperature under the field condition. Because the invention has the similar defects with the photosynthetic apparatus, the invention can be only used for quantitative detection of partial leaves and is not suitable for high-quality detection of the whole leaves.
Chinese patent application 201920309027.7, the title of the invention, "photosynthesis tester with automatic direction adjustment", discloses a tester, which comprises a base, wherein a photosynthesis tester is arranged on the base, the photosynthesis tester is arranged on the base through a turntable, a transmission gear is arranged at the lower end of the turntable, a rotating motor is arranged beside the base, a speed change gear is arranged on a rotating shaft of the rotating motor, the transmission gear is meshed with the speed change gear, a photosensitive sensor is arranged around the photosynthesis tester, the direction of real-time sunlight is detected through the photosensitive sensor, and the rotating motor is controlled to drive the turntable to rotate. Carry out photosynthesis through the photosynthesis apparatus and detect to drive the carousel level through rotating the motor and rotate, can't survey the blade of different angles.
The Chinese patent application 201910195243.8 entitled fast determination device for photosynthetic rate and transpiration rate of cotton boll leaf system discloses a rapid determination device comprising a plant photosynthetic determinator host, an IRGA sample chamber of an IRGA analyzer, and a cluster-shaped leaf chamber mounting plate, wherein the IRGA sample chamber of the IRGA analyzer is connected with the plant photosynthetic determinator host through a cable, and the rapid determination device is characterized in that a cover plate of the IRGA sample chamber of the IRGA analyzer is removed and connected with the cluster-shaped leaf chamber mounting plate, a self-made leaf chamber is mounted on the cluster-shaped leaf chamber mounting plate, and a vent hole is arranged on the connecting plate, so that the self-made leaf chamber, the cluster-shaped leaf chamber mounting plate and the IRGA sample chamber are communicated through the vent hole. Although the self-made blade chamber is circulated by the fan, the air in the blade chamber cannot be uniform, blades at different angles cannot be measured, the size of the blade chamber cannot be adjusted along with the size of the blades, and the self-made blade chamber is not suitable for high-precision measurement of a single blade.
Therefore, the measurement method using a classical Licor-6800 light combination measuring instrument or a similar improved device or the existing real-time online large-area photosynthetic detection method cannot meet the aim of accurately measuring the photosynthetic intensity generated by a single leaf in different growth directions (angles), and cannot ensure the stability of the carbon dioxide concentration in the long-time measurement process.
Disclosure of Invention
Therefore, the problem to be solved by the present invention is: at any time, carry out the photosynthesis survey to the plant leaf of different growth angles, can realize carrying out the accurate survey with the isolated outside air at best under the condition that does not influence the illumination of natural light, survey the photosynthesis intensity in-process for a long time and observe the carbon transfer orbit in not damaging the leaf to avoid the defect that prior art exists.
Therefore, the invention aims to overcome the defects of the existing equipment and provide the visual adjustable isotope labeling device for plant photosynthesis, which has the advantages of different growth angle leaves, high accuracy, no damage to the leaves and visualization.
In order to achieve the technical purpose, the invention enables air in the external blade chamber to circulate through a circulating pump on the basis of the traditional blade chamber or the external blade chamber, adopts a sealing ring to be isolated from the outside air, uses a light-transmitting material to solve the problem of light source by directly utilizing the irradiation of natural light, and fixes the external blade chamber for blades with different growth angles through a multi-angle fixing frame.
Therefore, one of the principles of the invention is that on the basis of measuring photosynthesis in the traditional leaf chamber, the air flow in the leaf chamber is circulated, so that the oxygen content and the carbon dioxide content of each part of the measured leaf are consistent, and the reduction of the photosynthesis intensity caused by different photosynthetic detection is avoided.
The second principle of the invention is that, in the technology of the traditional external circulation leaf chamber, the full-transparent material such as organic glass is used, the light source problem of photosynthesis can be solved by directly utilizing the irradiation of natural light, and the measurement of the photosynthesis of each leaf under the uniform illumination condition is realized by combining the isolation of a sealing ring and the outside air and avoiding the interference under the outside condition.
The third principle of the invention is that in order to ensure that the growth state of the leaves is not changed when the photosynthetic intensity is measured, the good growth state of the measured leaves and plants is more favorably kept, the leaves are prevented from being damaged or destroyed due to the measurement of photosynthesis, and the method is realized by fixing the external leaf chamber through the multi-angle fixing frame.
Accordingly, it is an object of the present invention to provide an adjustable isotope labeling apparatus for photosynthesis in plants, the apparatus comprising:
an external circulating leaf chamber 3 is formed by organic glass 1 and a sealing ring 2, the external circulating leaf chamber 3 is communicated with a carbon dioxide absorption tank 11 through an organic hose 13, the external circulating leaf chamber 3 is communicated with a circulating pump 4 through an organic hose 5, the external circulating leaf chamber 3 is fixed on a multi-angle fixing frame 6 through a metal support rod 7, a carbon dioxide concentration detection probe 8 is arranged in the external circulating leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with a carbon dioxide detector 10 through an optical fiber 9, the carbon dioxide absorption tank 11 is communicated with the circulating pump 4 through the organic hose, a carbon dioxide supply pipe 12 is communicated with the organic hose 5 through a three-way electronic control valve, the carbon dioxide flow rate is controlled electronically to ensure the supply of carbon dioxide, a carbon dioxide storage tank (or a generating tank) 15 is communicated with the three-, the organic hose 5 is in communication.
In any of the above embodiments, the organic glass 1 has a light transmittance of 80% or more, a thickness of 1-10mm, and a cylindrical, rectangular, spherical or the like shape.
In any of the above embodiments, the multi-angle fixing frame 6 is fixed at any angle.
In any of the above embodiments, the depth of the carbon dioxide concentration detection probe 8 placed in the external circulation leaf chamber 3 is 5-50 mm, preferably 15 mm.
In any of the above embodiments, the carbon dioxide absorption cell 11 is composed of 0.1-0.5 mol/L sodium hydroxide aqueous solution, and the amount of carbon dioxide absorbed is converted by measuring the change of pH in the solution or the amount of carbon dioxide generated by the neutralization reaction of sulfuric acid is directly measured, and the temperature of the solution can be controlled by a temperature reduction device, so as to directly influence the temperature change in the external circulation leaf chamber 3.
In any of the above embodiments, the carbon dioxide storage tank or generation tank 18, from a carbon dioxide storage tank with a pressure higher than 1 atm or from the reaction of sodium carbonate with dilute sulfuric acid, determines the discharge rate of carbon dioxide through the electronically controlled valve 14, and maintains the carbon dioxide concentration in the organic hose 5 at 0.03% in relation to the air circulation rate of the circulation pump 4, and then adjusts the carbon dioxide concentration appropriately according to the detection data of the carbon dioxide detector 10.
Drawings
FIG. 1 is a schematic view of an adjustable isotope labeling apparatus for photosynthesis in plants; wherein 1: organic glass; 2: a seal ring; 3: an external circulating leaf chamber; 4: a circulation pump; 5: an organic hose; 6: a multi-angle fixing frame; 7: a metal strut; 8: a carbon dioxide concentration detection probe; 9: an optical fiber; 10: a carbon dioxide detector; 11: a carbon dioxide absorption tank; 12: an organic hose; 13: a carbon dioxide supply pipe; 14: a three-way electronic control valve; 15: a carbon dioxide storage tank or a production tank.
Technical effects
1. The invention can make the carbon dioxide concentration in the leaf chamber completely the same through the external circulating pump, and avoids the uneven carbon dioxide concentration caused by photosynthesis in the leaf chamber.
2. When the multi-angle fixing frame is used for measuring the photosynthesis, the measurement of the photosynthetic intensity of the leaves at different growth angles of the plant cannot be influenced, the photosynthetic intensity of the leaves at different growth angles can be more conveniently compared in real time, the working intensity of operators is greatly reduced, and a very efficient tool is provided for basic research of plant photosynthesis and biological engineering research.
3. According to the invention, carbon dioxide brought by air is absorbed firstly, and carbon dioxide marked by the isotope is replenished at the later stage, so that an accurate carbon dioxide isotope marking experiment can be completely realized, and the risk of environmental pollution caused by isotope substances can be protected.
4. The invention can provide feasibility for a long-time test of photosynthetic strength of the whole plant or a single leaf, and can effectively slow down the temperature rise of the closed environment of a leaf chamber and effectively maintain the stability of the environmental humidity.
Detailed Description
The invention is further illustrated with reference to the following figures and examples. However, the following description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention will still fall within the scope of the technical solution of the present invention without departing from the technical solution of the present invention.
As shown in fig. 1, a schematic diagram of an adjustable isotope labeling apparatus for plant photosynthesis. Wherein, organic glass 1 and a sealing ring 2 are combined to form an external circulation leaf chamber 3, the external circulation leaf chamber 3 is communicated with a carbon dioxide absorption tank 11 through an organic hose 16 by a three-way valve 15, the external circulation leaf chamber 3 is communicated with a circulation pump 4 through an organic hose 5, the external circulation leaf chamber 3 is fixed on a multi-angle fixing frame 6 through a metal support rod 7, a carbon dioxide concentration detection probe 8 is arranged in the external circulation leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with a carbon dioxide detector 10 through an optical fiber 9, the carbon dioxide absorption tank 11 is communicated with the circulation pump 4 through an organic hose, a carbon dioxide supply pipe 12 is communicated with the organic hose 5 through a three-way electronic control valve, the flow rate of carbon dioxide is controlled by electrons to ensure the supply of carbon dioxide, a carbon dioxide storage tank (or a generating tank) 15 is communicated with, The organic hose 5 is in communication.
The first embodiment is as follows: method for detecting photosynthesis of rice leaves in real time by using any one of analysis systems
The method comprises the following steps: when the device is used, firstly, the sealing ring 2 is opened, rice leaves or the whole rice plant is selected to be placed into the organic glass 1, the organic glass 1 and the sealing ring 2 are combined to form the external circulating leaf chamber 3, the external circulating leaf chamber 3 is communicated with the carbon dioxide absorption pool 11 through the three-way valve 15 through the organic hose 16, the external circulating leaf chamber 3 is communicated with the circulating pump 4 through the organic hose 5, the external circulating leaf chamber 3 is fixed on the multi-angle fixing frame 6 through the metal support rod 7, the carbon dioxide concentration detection probe 8 is placed into the external circulating leaf chamber 3, the carbon dioxide concentration detection probe 8 is communicated with the carbon dioxide detector 10 through the optical fiber 9, the carbon dioxide absorption pool 11 is communicated with the circulating pump 4 through the organic hose, the carbon dioxide supply pipe 12 is communicated with the organic hose 5 through the three-way electronic control valve, the electronic control carbon dioxide flow rate ensures the carbon dioxide supply, the carbon dioxide storage The organic hose 5 is communicated with the leaf chamber, and the carbon dioxide concentration in the leaf chamber is controlled to be kept at 0.03% by detecting data through a carbon dioxide detector 10. The consumption amount of carbon dioxide in the whole photosynthetic process is calculated by measuring the pH change (amount of absorbed carbon dioxide) of the carbon dioxide absorption cell 11 and the input amount of the carbon dioxide supply tube 13 (amount of supplied carbon dioxide).
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (6)
1. An adjustable isotope labeling apparatus for photosynthesis in plants, the apparatus comprising: organic glass 1 and sealing washer 2 constitute external circulation leaf room 3, external circulation leaf room 3 communicates with carbon dioxide absorption cell 11 through organic hose 13, external circulation leaf room 3 communicates with circulating pump 4 through organic hose 5, external circulation leaf room 3 is fixed on multi-angle mount 6 through metal branch 7, carbon dioxide concentration test probe 8 is put into in external circulation leaf room 3, carbon dioxide concentration test probe 8 is linked together with carbon dioxide detector 10 through optic fibre 9, carbon dioxide absorption cell 11 communicates with circulating pump 4 through organic hose, carbon dioxide supply pipe 12 communicates with organic hose 5 through tee bend electronic control valve, the carbon dioxide is supplied with to electronic control carbon dioxide velocity of flow assurance, carbon dioxide holding vessel 15 communicates with tee bend electronic control valve, organic hose 5 through carbon dioxide supply pipe 12.
2. The device of claim 1, wherein the organic glass 1 has a light transmittance of 80% or more, a thickness of 1-10mm, and a cylindrical, rectangular, spherical shape or the like.
3. The apparatus according to claim 1, wherein said multi-angle fixing frame 6 is a fixed frame with any angle.
4. The device according to claim 1, wherein the depth of the carbon dioxide concentration detection probe 8 placed in the external circulation leaf chamber 3 is 5-50 mm, preferably 15 mm.
5. The device according to claim 1, wherein the carbon dioxide absorption cell 11 comprises 0.1-0.5 mol/L sodium hydroxide aqueous solution, the absorption amount of carbon dioxide is calculated by measuring the change of pH value in the solution or the amount of carbon dioxide generated by sulfuric acid neutralization reaction is directly measured, and the temperature of the solution can be controlled by a temperature reduction device to directly influence the temperature change in the external circulation leaf chamber 3.
6. The apparatus of claim 1, wherein the carbon dioxide storage tank or generation tank 18, which is used for producing carbon dioxide from carbon dioxide storage tank with pressure higher than 1 atm or from the reaction of sodium carbonate and dilute sulfuric acid, is controlled by the electronically controlled valve 14 to determine the discharge rate of carbon dioxide, and the discharge rate is related to the air circulation rate of the circulation pump 4, and the carbon dioxide concentration in the organic hose 5 is maintained at 0.03%, and then is appropriately adjusted according to the detection data of the carbon dioxide detector 10.
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| CN202110450340.4A CN113063903A (en) | 2021-04-25 | 2021-04-25 | Adjustable isotope labeling device for plant photosynthesis |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113600102A (en) * | 2021-07-30 | 2021-11-05 | 中国辐射防护研究院 | C-14 and H-3 source projection device and method in airborne nuclide environment migration test |
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