CN103598025A

CN103598025A - Field in-situ continuous low-abundance 13CO2 isotope labeling device and method

Info

Publication number: CN103598025A
Application number: CN201310513820.6A
Authority: CN
Inventors: 乔云发; 苗淑杰; 高淑杰
Original assignee: Northeast Institute of Geography and Agroecology of CAS
Current assignee: Northeast Institute of Geography and Agroecology of CAS
Priority date: 2013-10-28
Filing date: 2013-10-28
Publication date: 2014-02-26
Anticipated expiration: 2033-10-28
Also published as: CN103598025B

Abstract

The invention discloses a field in-situ continuous low-abundance 13CO2 isotope labeling device and method, relates to a simulator for detecting crop rhizosphere respiration and labeling plants, and solves the problems of low labeling precision and high cost of a pulse labeling mode of a traditional C element labeling device or method for the plants. A labeling box is a transparent box with a through hole in the side wall, a combustion end of a combustor penetrates the through hole of the labeling box and extends into the labeling box, the tail end of the combustor is simultaneously communicated with a gas outlet of a liquefied gas bottle and a gas outlet of an oxygen bottle, an electronic fire maker is arranged at the combustion end of the combustor, a fan is fixed to the middle of the inner top of the labeling box, an air conditioner is fixed to the upper portion of the side wall of the labeling box, a CO2 measurement and control device and a humidifier are positioned in the labeling box, a dehumidifier is fixed to the lower portion of the side wall of the labeling box, and the fan, the air conditioner, the CO2 measurement and control device, the electronic fire maker, the dehumidifier and the humidifier are controlled by an automatic control system. The device and the method are used for performing continuous low-abundance 13CO2 labeling for crops.

Description

The continuous low abundance of a kind of field original position 13cO 2tagging device and method

Technical field

The present invention relates to a kind of detection crop rhizosphere breathes and mark plant analogue means.

Background technology

In the past, people adopt high abundance conventionally ¹³cO ₂carry out pulsed mark, few people adopt high abundance ¹³cO ₂continued labelling, discontinuous to making substance markers, mark cost is large, and detection means falls behind, and error is large, and precision is low.Now, along with the increase of scientific and technological progress and atmosphere percent of greenhouse gases, people update distribution research method and the means of crop photosynthesis product, and people recognize clear-headedly and utilize continuously ¹³cO ₂the importance of mark, it determines the fixedly CO in atmosphere of crop ₂by redistributing the capability of influence to the ecosystem.So the researcher of greenhouse gases both domestic and external and plant physiology aspect is to utilizing continuously ¹³cO ₂the research of mark is paid much attention to. ¹³c continued labelling technology is more and more engaged in focus and the breach of carbon research work, and its continued labelling method relatively lags behind, and mark cost is high, few people's research.

Traditional method generally adopts high abundance ¹¹cO ₂, ¹³cO ₂with ¹⁴cO ₂three kinds of mark CO ₂gas carries out mark to crop, because label cost is higher, conventionally adopts pulsed mark, and in measuring process, disturbing factor is more, and error is large, and the mark time is shorter, makes some less different can't detect, and part is out in the cold.

Summary of the invention

The present invention adopts pulsed mark mode in order to solve device or the method for traditional C rubidium marking that plant is carried out, and mark precision is low, and the problem that cost is high the invention provides the continuous low abundance of a kind of field original position ¹³cO ₂tagging device and method.

The continuous low abundance of a kind of field original position ¹³cO ₂tagging device, is characterized in that, it comprises mark case, fan, air-conditioning, burner, CO ₂controller, oxygen cylinder, liquefied gas bottle, dehumidifier, automatic control system and humidifier; Described mark case is bottom opening formula casing, the sidewall of described mark case is provided with a through hole, described mark case is transparent casing, the fire end of burner stretches into mark case inside through the through hole of mark case, the end of described burner is communicated with the gas outlet of liquefied gas bottle and the gas outlet of oxygen cylinder simultaneously, and the fire end of described burner is provided with electronic lighter; Fan is fixed on mark case inner top centre position, and air-conditioning is fixed on the top of mark case sidewall, CO ₂controller is positioned at mark case, dehumidifier is fixed on the bottom of mark case sidewall, humidifier is placed in mark case, it is outside that automatic control system is placed on mark case, described automatic controlling system signal output part while and ON/OFF control signal input, the control signal input of air-conditioning, the CO of fan ₂the control signal input of controller, the control signal input of electronic lighter, the control signal input of dehumidifier and the control signal input of humidifier are connected.

In the liquefied gas of described liquefied gas bottle output ¹³c abundance ratio atmospheric background is low by 60%.

The continuous low abundance of a kind of field of the present invention original position ¹³cO ₂tagging device, is mainly used in studying the fixedly CO in atmosphere of crop photosynthesis ₂distribution and rhizosphere respiration CO at crop plant, rhizosphere respiration ₂the system and device that emission flux combines together.Its major function is to adopt low abundance ¹³cO ₂continue mark.

Adopt the continuous low abundance of a kind of field original position ¹³cO ₂tagging device is realized the continuous low abundance of a kind of field original position ¹³cO ₂isotope labelling method, its concrete steps are,

First, mark case be placed on to plantation have on the soil of crop to be marked, and make described crop to be marked be positioned at mark case, the humidification port of humidifier is contacted with the interior soil of mark case, make natural daylight see through mark case and irradiate crop to be marked;

Then, in the process of growth of crop to be marked:

According to the parameter of the required growing environment of crop to be marked, by automatic controlling system operation of air conditioner, make mark the temperature inside the box within the scope of 20～25 ℃; By automatic controlling system humidifier and dehumidifier, make humidity in mark case in 50～60% scopes;

Pass through CO ₂low abundance in controller monitoring mark case ¹³cO ₂concentration, low abundance in mark case ¹³cO ₂concentration during lower than 350ppm, makes the oxygen of oxygen cylinder output and the low abundance of liquefied gas burning acquisition of liquefied gas bottle output by automatic controlling system electronic lighter ¹³cO ₂, control fan running simultaneously, make low abundance in mark case ¹³cO ₂be uniformly distributed; Low abundance in mark case ¹³cO ₂concentration during higher than 450ppm, quits work burner; Make low abundance in mark case ¹³cO ₂concentration keeps X hour being greater than 350ppm or being less than between 450ppm, with the low abundance of tense marker crop to be marked ¹³cO ₂tagging, completes the growth tagging of the crop to be marked in X hour, and described X represents the isotopic mark time, and the scope of X is for being greater than 10.

The present invention takes in the liquefied gas of liquefied gas bottle output ¹³the feature of C abundance ratio atmospheric background low 60%, by the CO of liquefied gas and purity oxygen burning generation ₂gas serves as a mark ¹³cO ₂, utilize crop photosynthesis fixation of C O ₂feature, engage under natural daylight growth conditions, by the simulation climate chamber of automatic controlling system mark case temp. and humidity, and carbon process and crop plant labeling process combine together admittedly entirely by crop photosynthesis, can continue mark to crop, the mark time is long, mark cost 70%, the marked product data of distributing are accurate, mark precision is high more than 25%.

Accompanying drawing explanation

Fig. 1 is the continuous low abundance of a kind of field of the present invention original position ¹³cO ₂the principle schematic of tagging device.

Embodiment

Embodiment one: referring to Fig. 1, present embodiment is described, the continuous low abundance of a kind of field original position described in present embodiment ¹³cO ₂tagging device, it comprises mark case 1, fan 2, air-conditioning 3, burner 4, CO ₂controller 5, oxygen cylinder 7, liquefied gas bottle 8, dehumidifier 9, automatic control system 10 and humidifier 11; Described mark case 1 is bottom opening formula casing, the sidewall of described mark case 1 is provided with a through hole, described mark case 1 is transparent casing, the fire end of burner 4 stretches into mark case 1 inside through the through hole of mark case 1, the end of described burner 4 is communicated with the gas outlet of liquefied gas bottle 8 and the gas outlet of oxygen cylinder 7 simultaneously, and the fire end of described burner 4 is provided with electronic lighter 6; Fan 2 is fixed on mark case 1 inner top centre position, and air-conditioning 3 is fixed on the top of mark case 1 sidewall, CO ₂controller 5 is positioned at mark case 1, dehumidifier 9 is fixed on the bottom of mark case 1 sidewall, humidifier 11 is placed in mark case 1, automatic control system 10 is placed on mark case 1 outside, described 10 control signal outputs whiles of automatic control system and ON/OFF control signal input, the control signal input of air-conditioning 3, the CO of fan 2 ₂the control signal input of controller 5, the control signal input of electronic lighter 6, the control signal input of dehumidifier 9 and the control signal input of humidifier 11 are connected.

Embodiment two: referring to Fig. 1, present embodiment is described, the continuous low abundance of a kind of field original position described in present embodiment and embodiment one ¹³cO ₂the difference of tagging device is, described CO ₂controller 5 is positioned at mark case 1 bosom position.

Embodiment three: referring to Fig. 1, present embodiment is described, the continuous low abundance of a kind of field original position described in present embodiment and embodiment one or two ¹³cO ₂the difference of tagging device is, described air-conditioning 3 and dehumidifier 9 are arranged on the same sidewall of mark case 1.

Embodiment four: referring to Fig. 1, present embodiment is described, the continuous low abundance of a kind of field original position described in present embodiment embodiment one ¹³cO ₂the difference of tagging device is, in the liquefied gas of described liquefied gas bottle 8 outputs ¹³c abundance ratio atmospheric background is low by 55% to 65%.

Embodiment five: referring to Fig. 1, present embodiment is described, the continuous low abundance of a kind of field original position described in present embodiment embodiment four ¹³cO ₂the difference of tagging device is, in the liquefied gas of described liquefied gas bottle 8 outputs ¹³c abundance ratio atmospheric background is low by 60%.

Embodiment six: referring to Fig. 1, present embodiment is described, adopts the continuous low abundance of a kind of field original position described in embodiment one, two, four or five ¹³cO ₂tagging device is realized the continuous low abundance of a kind of field original position ¹³cO ₂isotope labelling method, its concrete steps are,

First, mark case 1 be placed on to plantation have on the soil of crop to be marked, and make described crop to be marked be positioned at mark case 1, the humidification port of humidifier 11 is contacted with the interior soil of mark case 1, make natural daylight see through mark case 1 irradiation crop to be marked;

Then, in the process of growth of crop to be marked:

According to the parameter of the required growing environment of crop to be marked, by automatic control system 10, control air-conditioning 3 operations, make the interior temperature of mark case 1 within the scope of 20～25 ℃; By automatic control system 10, control humidifier 11 and dehumidifiers 9, make humidity in mark case in 50～60% scopes;

Pass through CO ₂the interior low abundance of controller 5 monitoring mark case 1 ¹³cO ₂concentration, when the interior low abundance of mark case 1 ¹³cO ₂concentration during lower than 350ppm, is controlled electronic lighters 6 by automatic control system 10 and is made the oxygen of oxygen cylinder 7 outputs and the low abundance of liquefied gas burning acquisition of liquefied gas bottle 8 outputs ¹³cO ₂, control fan 2 runnings simultaneously, make the interior low abundance of mark case 1 ¹³cO ₂be uniformly distributed; When the interior low abundance of mark case 1 ¹³cO ₂concentration during higher than 450ppm, quits work burner 4; Make the interior low abundance of mark case 1 ¹³cO ₂concentration keeps X hour being greater than 350ppm or being less than between 450ppm, with the low abundance of tense marker crop to be marked ¹³cO ₂tagging, completes the growth tagging of the crop to be marked in X hour, and described X represents the isotopic mark time, and the scope of X is for being greater than 10.

Embodiment seven: referring to Fig. 1, present embodiment is described, according to the continuous low abundance of a kind of field original position described in embodiment six ¹³cO ₂the difference of isotope labelling method is, the scope of described X is for being greater than 12 and be less than 1000.

Claims

1. the continuous low abundance of field original position ¹³cO ₂tagging device, is characterized in that, it comprises mark case (1), fan (2), air-conditioning (3), burner (4), CO ₂controller (5), oxygen cylinder (7), liquefied gas bottle (8), dehumidifier (9), automatic control system (10) and humidifier (11); Described mark case (1) is bottom opening formula casing, the sidewall of described mark case (1) is provided with a through hole, described mark case (1) is transparent casing, the fire end of burner (4) stretches into mark case (1) inside through the through hole of mark case (1), the end of described burner (4) is communicated with the gas outlet of liquefied gas bottle (8) and the gas outlet of oxygen cylinder (7) simultaneously, and the fire end of described burner (4) is provided with electronic lighter (6); Fan (2) is fixed on mark case (1) inner top centre position, and air-conditioning (3) is fixed on the top of mark case (1) sidewall, CO ₂controller (5) is positioned at mark case (1), dehumidifier (9) is fixed on the bottom of mark case (1) sidewall, humidifier (11) is placed in mark case (1), automatic control system (10) is placed on mark case (1) outside, described automatic control system (10) control signal output while and the ON/OFF control signal input of fan (2), control signal input, the CO of air-conditioning (3) ₂the control signal input of the control signal input of the control signal input of controller (5), electronic lighter (6), dehumidifier (9) is connected with the control signal input of humidifier (11).

2. the continuous low abundance of a kind of field according to claim 1 original position ¹³cO ₂tagging device, is characterized in that, described CO ₂controller (5) is positioned at mark case (1) bosom position.

3. the continuous low abundance of a kind of field according to claim 1 and 2 original position ¹³cO ₂tagging device, is characterized in that, described air-conditioning (3) and dehumidifier (9) are arranged on the same sidewall of mark case (1).

4. the continuous low abundance of a kind of field according to claim 1 original position ¹³cO ₂tagging device, is characterized in that, in the liquefied gas of described liquefied gas bottle (8) output ¹³c abundance ratio atmospheric background is low by 55% to 65%.

5. the continuous low abundance of a kind of field according to claim 4 original position ¹³cO ₂tagging device, is characterized in that, in the liquefied gas of described liquefied gas bottle (8) output ¹³c abundance ratio atmospheric background is low by 60%.

6. adopt the continuous low abundance of a kind of field original position described in claim 1,2,4 or 5 ¹³cO ₂tagging device is realized the continuous low abundance of a kind of field original position ¹³cO ₂isotope labelling method, is characterized in that, its concrete steps are,

First, mark case (1) being placed on to plantation has on the soil of crop to be marked, and make described crop to be marked be positioned at mark case (1), the humidification port of humidifier (11) is contacted with the interior soil of mark case (1), make natural daylight see through mark case (1) and irradiate crop to be marked;

Then, in the process of growth of crop to be marked:

According to the parameter of the required growing environment of crop to be marked, by automatic control system (10), control air-conditioning (3) operation, make the interior temperature of mark case (1) within the scope of 20～25 ℃; By automatic control system (10), control humidifier (11) and dehumidifier (9), make humidity in mark case in 50～60% scopes;

Pass through CO ₂low abundance in controller (5) monitoring mark case (1) ¹³cO ₂concentration, low abundance in mark case (1) ¹³cO ₂concentration during lower than 350ppm, is controlled electronic lighter (6) by automatic control system (10) and is made the oxygen of oxygen cylinder (7) output and the low abundance of liquefied gas burning acquisition of liquefied gas bottle (8) output ¹³cO ₂, control fan (2) running simultaneously, make low abundance in mark case (1) ¹³cO ₂be uniformly distributed; Low abundance in mark case (1) ¹³cO ₂concentration during higher than 450ppm, quits work burner (4); Make low abundance in mark case (1) ¹³cO ₂concentration keeps X hour being greater than 350ppm or being less than between 450ppm, with the low abundance of tense marker crop to be marked ¹³cO ₂tagging, completes the growth tagging of the crop to be marked in X hour, and described X represents the isotopic mark time, and the scope of X is for being greater than 10.

7. the continuous low abundance of a kind of field according to claim 6 original position ¹³cO ₂isotope labelling method, is characterized in that, the scope of described X is for being greater than 12 or be less than 1000.