CN108093930A - A kind of photosynthetic plant tracer case - Google Patents

A kind of photosynthetic plant tracer case Download PDF

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Publication number
CN108093930A
CN108093930A CN201711319499.2A CN201711319499A CN108093930A CN 108093930 A CN108093930 A CN 108093930A CN 201711319499 A CN201711319499 A CN 201711319499A CN 108093930 A CN108093930 A CN 108093930A
Authority
CN
China
Prior art keywords
plant
inner casing
plant leaf
connecting hole
photosynthetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711319499.2A
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Chinese (zh)
Inventor
张文旭
陶镜合
张喜萍
梁莉
陆坤
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Northwest Normal University
Original Assignee
Northwest Normal University
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Filing date
Publication date
Application filed by Northwest Normal University filed Critical Northwest Normal University
Priority to CN201711319499.2A priority Critical patent/CN108093930A/en
Publication of CN108093930A publication Critical patent/CN108093930A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/14Greenhouses
    • A01G9/16Dismountable or portable greenhouses ; Greenhouses with sliding roofs
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/24Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to vegetal tests technical fields, particularly a kind of photosynthetic plant tracer case.The isotope distribution of C and transhipment research suitable for photosynthesis of plant.Including babinet, root culture ware, hanging plate, plant leaf sealing inner casing, light bar and condenser pipe, root culture ware is located at the bottom of box, multiple plant leaf sealing inner casings are set on hanging plate, plant leaf sealing inner shell bottom is equipped with connecting hole, plant leaf is put into plant leaf sealing inner casing, and root system is embedded to root culture ware by connecting hole.Plant leaf is sealed by inner casing and avoids external interference, promotes measuring accuracy.By hanging plate multiple plant leafs is set to seal the transport experiment that inner casing realizes a variety of C element isotopes.

Description

A kind of photosynthetic plant tracer case
Technical field
The present invention relates to vegetal tests technical fields, particularly a kind of photosynthetic plant tracer case.Suitable for Plant Light cooperation With the isotope distribution of middle C and transhipment research.
Background technology
The 90%~95% of crop biology yield comes from the photosynthetic product of plant, CO2Crop photosynthesis Reactant plays conclusive effect in the photosynthesis research of crop.With the isotope of C(12CO213CO214CO2)Tracer The photosynthetic organs of plant study distribution and transhipment of the C element in crop, contribute to the photosynthesis of in-depth study plant, And corresponding technology is taken to improve crop biology yield on its basis.
At present in research, not having can control experiment under intensity of illumination, temperature and equal conditions to repeat used set It is standby, larger error is be easy to cause, be easy to cause the judgement of mistake.
The content of the invention
The present invention solves prior art deficiency and provides the photosynthetic plant tracer case that a kind of test is accurate, external interference is few.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of photosynthetic plant tracer case, including babinet, root culture ware, hanging plate, plant leaf sealing inner casing, light bar and condensation Pipe, root culture ware are located at the bottom of box, and multiple plant leaf sealing inner casings, plant leaf sealing inner shell bottom are mounted on hanging plate Equipped with connecting hole, plant leaf is put into plant leaf sealing inner casing, and root system is embedded to root culture ware by connecting hole.
The plant leaf sealing inner casing includes reaction vessels and fluid injector, and isotope charcoal is put into reaction vessels Block injects sulfuric acid by fluid injector.
The connecting hole of the plant leaf sealing inner casing is equipped with seal rubber plug, seal rubber plug clamping plant limb.
Multiple light bar and condenser pipe are equipped in the babinet.
The babinet top is equipped with multiple light bar, is equidistantly laid between light bar.
Multiple condenser pipes are equipped in the babinet, are equidistantly laid between condenser pipe.
The plant leaf sealing inner casing is divided into shell and pedestal, and shell is connected with pedestal;Pedestal is equipped with connecting hole.
The babinet covering black-out cloth or PVC board.
A kind of application method of photosynthetic plant tracer case, includes the following steps:
A, plant leaf is put into plant leaf by connecting hole to seal in inner casing, seal rubber plug is placed on connecting hole and forms inner casing Sealing, seal rubber plug clamping plant limb;
B, isotope carbon block is put into reaction vessels, injecting sulfuric acid reaction by fluid injector generates carbon dioxide, plants Strain blade absorbing carbon dioxide gas;
C, the quantity and spacing of light bar and condenser pipe are adjusted, intensity of illumination and temperature in control cabinet body;
D, plant leaf sealing inner casing is mounted on hanging plate;
E, in plant root embedment root culture ware.
The joint place smearing vaseline of seal rubber plug, seal rubber plug and connecting hole is placed in the step A on connecting hole.
Beneficial effects of the present invention are:
1st, a kind of photosynthetic plant tracer case, including babinet, root culture ware, hanging plate, plant leaf sealing inner casing, light bar and condensation Pipe, root culture ware are located at the bottom of box, and multiple plant leaf sealing inner casings, plant leaf sealing inner shell bottom are set on hanging plate Equipped with connecting hole, plant leaf is put into plant leaf sealing inner casing, and root system is embedded to root culture ware by connecting hole.By interior Shell sealing plant leaf avoids external interference, promotes measuring accuracy.By hanging plate multiple plant leafs is set to seal inner casing to realize The transport experiment of a variety of C element isotopes.This equipment can be with repeated tests, and it is close that plant by connecting hole penetrates plant leaf It seals in inner casing, reduces equipment use cost.
2nd, plant leaf sealing inner casing includes reaction vessels and fluid injector, and isotope carbon block is put into reaction vessels, Sulfuric acid is injected by fluid injector.Pass through carbon block and sulfuric acid chemical reaction generation CO2Environment.
3rd, sealing piston is connected on the connecting hole of plant leaf sealing inner casing.Realize that plant root is pierced by by connecting hole. Sealing piston seals to form independent sealed cavity.
The 4th, multiple light bar and condenser pipe are set in babinet.Intensity of illumination and temperature in control cabinet body.
Description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the structural diagram of the present invention;
Fig. 2 is side view of the present invention;
Fig. 3 is the top view of the present invention;
Fig. 4 is the structure diagram that plant leaf seals inner casing.
Specific embodiment
In figure, a kind of photosynthetic plant tracer case, including babinet 1, root culture ware 2, hanging plate 3, plant leaf sealing inner casing 4th, light bar 5 and condenser pipe 6, root culture ware 2 are located at 1 bottom of babinet, and multiple plant leaf sealing inner casings 4 are mounted on hanging plate 3, are planted 4 bottom of strain runner sealing inner casing is equipped with connecting hole 9, and plant leaf is put into plant leaf sealing inner casing 4, and root system passes through connecting hole It is embedded to root culture ware 2.
The plant leaf sealing inner casing 4 includes reaction vessels 7 and fluid injector 8, and isotope is put into reaction vessels 7 Carbon block injects sulfuric acid by fluid injector 8.The connecting hole 9 of the plant leaf sealing inner casing 4 is equipped with seal rubber plug 10, Seal rubber plug 10 clamps plant limb.Multiple light bar 5 and condenser pipe 6 are equipped in the babinet 1.1 top of babinet is equipped with more A light bar 5 is equidistantly laid between light bar 5.Multiple condenser pipes 6 are equipped in the babinet 1, are equidistantly laid between condenser pipe 6. Plant leaf sealing inner casing 4 divides for shell 11 and pedestal 12, and shell 11 and pedestal 12 connect;Pedestal 12 is equipped with connecting hole 9。
Its middle case 1 covers lighttight black cloth, black-out cloth or PVC board, and 5 model of light bar selects Philips lifemax TLD 30W/54-765 Cool DAY LIGHT、provided a photon flux close to 3×1825 lm, and 61 lm/W;
In use, babinet covers lighttight black cloth, 3 fluorescent tubes provide light source, control the flow-rate adjustment case of water in 7 condenser pipes Plant leaf is put into plant leaf by connecting hole and sealed in inner casing, seal rubber plug is placed on connecting hole and is formed by interior temperature Inner casing seals, seal rubber plug clamping plant limb;Isotope carbon block is put into reaction vessels, sulfuric acid is injected by fluid injector Reaction generates carbon dioxide, plant leaf absorbing carbon dioxide gas;Adjust light bar and the quantity and spacing of condenser pipe, control Intensity of illumination and temperature in babinet processed;Plant leaf sealing inner casing is mounted on hanging plate;In plant root embedment root culture ware.
The joint place smearing vaseline of seal rubber plug, seal rubber plug and connecting hole is placed on the connecting hole.Further carry Rise gas seal property in housing.Plant leaf isotope carbon dioxide takes out plant analysis after a period of time is reacted Study distribution and transhipment situation of the C element in crop.

Claims (10)

1. a kind of photosynthetic plant tracer case, it is characterised in that including babinet(1), root culture ware(2), hanging plate(3), plant leaf Seal inner casing(4), light bar(5)And condenser pipe(6), root culture ware(2)It is located at babinet(1)Bottom, hanging plate(3)On mount it is multiple Plant leaf seals inner casing(4), plant leaf sealing inner casing(4)Bottom is equipped with connecting hole(9), plant leaf is put into plant leaf Seal inner casing(4)In, root system is embedded to root culture ware by connecting hole(2).
2. a kind of photosynthetic plant tracer case according to claim 1, it is characterised in that the plant leaf seals inner casing(4)Bag Include reaction vessels(7)And fluid injector(8), reaction vessels(7)Isotope carbon block is inside put into, passes through fluid injector(8)Note Enter sulfuric acid.
3. a kind of photosynthetic plant tracer case according to claim 1, it is characterised in that the plant leaf seals inner casing(4)'s Connecting hole(9)It is equipped with seal rubber plug(10), seal rubber plug(10)Clamp plant limb.
A kind of 4. photosynthetic plant tracer case according to claim 1, it is characterised in that the babinet(1)It is interior to be equipped with multiple light bar (5)And condenser pipe(6).
A kind of 5. photosynthetic plant tracer case according to claim 4, it is characterised in that the babinet(1)Top is equipped with multiple lamps Band(5), light bar(5)Between equidistantly lay.
A kind of 6. photosynthetic plant tracer case according to claim 4, it is characterised in that the babinet(1)It is interior to be equipped with multiple condensations Pipe(6), condenser pipe(6)Between equidistantly lay.
7. a kind of photosynthetic plant tracer case according to claim 3, it is characterised in that the plant leaf seals inner casing(4)Point For shell(11)And pedestal(12), shell(11)And pedestal(12)Connection;Pedestal(12)It is equipped with connecting hole(9).
A kind of 8. photosynthetic plant tracer case according to claim 1, it is characterised in that the babinet(1)Cover black-out cloth or PVC board.
9. according to a kind of application method of photosynthetic plant tracer case of claim 1 to 8 any one of them, it is characterised in that including Following steps:
A, connecting hole is passed through(9)Plant leaf is put into plant leaf sealing inner casing(4)It is interior, connecting hole(9)Upper placement fluid sealant Plug(10)Form inner casing sealing, seal rubber plug(10)Clamp plant limb;
B, reaction vessels(7)Isotope carbon block is inside put into, passes through fluid injector(8)It injects sulfuric acid reaction and generates carbon dioxide gas Body, plant leaf absorbing carbon dioxide gas;
C, light bar is adjusted(5)And condenser pipe(6)Quantity and spacing, control babinet(1)Interior intensity of illumination and temperature;
D, plant leaf sealing inner casing(4)It is mounted on hanging plate(3)On;
E, plant root embedment root culture ware(2)It is interior.
10. the application method of a kind of photosynthetic plant tracer case according to claim 9, it is characterised in that in the step A Connecting hole(9)Upper placement seal rubber plug(10), seal rubber plug(10)And connecting hole(9)Joint place smear vaseline.
CN201711319499.2A 2017-12-12 2017-12-12 A kind of photosynthetic plant tracer case Pending CN108093930A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711319499.2A CN108093930A (en) 2017-12-12 2017-12-12 A kind of photosynthetic plant tracer case

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711319499.2A CN108093930A (en) 2017-12-12 2017-12-12 A kind of photosynthetic plant tracer case

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CN108093930A true CN108093930A (en) 2018-06-01

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109258175A (en) * 2018-10-29 2019-01-25 杭州师范大学 Isotope trickle irrigation culture apparatus
CN111751500A (en) * 2020-07-06 2020-10-09 吉林建筑大学 Plant carbon isotope labeling device and plant carbon isotope labeling method
CN114674974A (en) * 2022-02-09 2022-06-28 山东省农业科学院 Drainage device of corn leaf sealing cover

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101015248A (en) * 2006-07-28 2007-08-15 中国科学院亚热带农业生态研究所 Method and device for plants carbon isotope labelling
CN201894089U (en) * 2010-11-16 2011-07-13 香港城市大学深圳研究院 Device for adopting stable carbon isotope gas for marking plants
JP2012075433A (en) * 2010-09-30 2012-04-19 Miyama Giken:Kk Photosynthesis experiment unit
CN102440153A (en) * 2011-09-09 2012-05-09 中国科学院东北地理与农业生态研究所 Pulse type device for conducting quantitative marking on plant with <13>CO2, and method for marking plant by utilizing same
CN103598025A (en) * 2013-10-28 2014-02-26 中国科学院东北地理与农业生态研究所 Field in-situ continuous low-abundance 13CO2 isotope labeling device and method
CN103698476A (en) * 2013-12-19 2014-04-02 中国计量学院 Isotopic tracer method for determining carbon sources and carbon sinks of perennial vegetation in situ
CN203998964U (en) * 2014-07-15 2014-12-10 西北农林科技大学 Plant organ carbon isotope photosynthesis labelled CO 2preparation facilities
CN204269629U (en) * 2014-11-28 2015-04-15 中国农业大学 A kind of carbon isotope labelling device
CN204903503U (en) * 2015-08-21 2015-12-23 中国科学院南京土壤研究所 Carbon isotope mark device
CN206365334U (en) * 2016-12-31 2017-08-01 中国农业科学院农业资源与农业区划研究所 A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample
CN207543710U (en) * 2017-12-12 2018-06-29 西北师范大学 A kind of photosynthetic plant tracer case

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101015248A (en) * 2006-07-28 2007-08-15 中国科学院亚热带农业生态研究所 Method and device for plants carbon isotope labelling
JP2012075433A (en) * 2010-09-30 2012-04-19 Miyama Giken:Kk Photosynthesis experiment unit
CN201894089U (en) * 2010-11-16 2011-07-13 香港城市大学深圳研究院 Device for adopting stable carbon isotope gas for marking plants
CN102440153A (en) * 2011-09-09 2012-05-09 中国科学院东北地理与农业生态研究所 Pulse type device for conducting quantitative marking on plant with <13>CO2, and method for marking plant by utilizing same
CN103598025A (en) * 2013-10-28 2014-02-26 中国科学院东北地理与农业生态研究所 Field in-situ continuous low-abundance 13CO2 isotope labeling device and method
CN103698476A (en) * 2013-12-19 2014-04-02 中国计量学院 Isotopic tracer method for determining carbon sources and carbon sinks of perennial vegetation in situ
CN203998964U (en) * 2014-07-15 2014-12-10 西北农林科技大学 Plant organ carbon isotope photosynthesis labelled CO 2preparation facilities
CN204269629U (en) * 2014-11-28 2015-04-15 中国农业大学 A kind of carbon isotope labelling device
CN204903503U (en) * 2015-08-21 2015-12-23 中国科学院南京土壤研究所 Carbon isotope mark device
CN206365334U (en) * 2016-12-31 2017-08-01 中国农业科学院农业资源与农业区划研究所 A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample
CN207543710U (en) * 2017-12-12 2018-06-29 西北师范大学 A kind of photosynthetic plant tracer case

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109258175A (en) * 2018-10-29 2019-01-25 杭州师范大学 Isotope trickle irrigation culture apparatus
CN111751500A (en) * 2020-07-06 2020-10-09 吉林建筑大学 Plant carbon isotope labeling device and plant carbon isotope labeling method
CN114674974A (en) * 2022-02-09 2022-06-28 山东省农业科学院 Drainage device of corn leaf sealing cover

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Application publication date: 20180601