CN206365334U - A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample - Google Patents
A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample Download PDFInfo
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- CN206365334U CN206365334U CN201621485596.XU CN201621485596U CN206365334U CN 206365334 U CN206365334 U CN 206365334U CN 201621485596 U CN201621485596 U CN 201621485596U CN 206365334 U CN206365334 U CN 206365334U
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 92
- 238000002372 labelling Methods 0.000 title claims abstract description 49
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 46
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 45
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 239000012530 fluid Substances 0.000 claims abstract description 45
- 238000012258 culturing Methods 0.000 claims abstract description 40
- 239000007789 gas Substances 0.000 claims abstract description 40
- 230000001954 sterilising effect Effects 0.000 claims abstract description 37
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 239000004065 semiconductor Substances 0.000 claims abstract description 28
- 235000016709 nutrition Nutrition 0.000 claims abstract description 26
- 230000035764 nutrition Effects 0.000 claims abstract description 26
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 23
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 23
- 238000009833 condensation Methods 0.000 claims abstract description 21
- 230000005494 condensation Effects 0.000 claims abstract description 21
- 239000006004 Quartz sand Substances 0.000 claims abstract description 16
- 235000015097 nutrients Nutrition 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000002699 waste material Substances 0.000 claims description 7
- 238000012549 training Methods 0.000 claims description 6
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229960001866 silicon dioxide Drugs 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- 235000013311 vegetables Nutrition 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 20
- 229910002092 carbon dioxide Inorganic materials 0.000 description 33
- 239000000243 solution Substances 0.000 description 23
- 239000000523 sample Substances 0.000 description 21
- 239000002689 soil Substances 0.000 description 15
- 230000008569 process Effects 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 238000011534 incubation Methods 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 239000010902 straw Substances 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 125000004122 cyclic group Chemical group 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000005679 Peltier effect Effects 0.000 description 2
- 150000001721 carbon Chemical group 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000007791 dehumidification Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004158 soil respiration Methods 0.000 description 2
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 206010010264 Condition aggravated Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920005479 Lucite® Polymers 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 125000004433 nitrogen atom Chemical group N* 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model is related to isotope labelling techniques field, more particularly to a kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample, including closed culturing room, CO2Syringe, gas balance device, nutrition liquid syringe and nutrition fluid circulation；Closed culturing room includes setting semi-conductor condensation dehydrating unit in translucent cover and base, translucent cover, translucent cover connection CO2Syringe and gas bascule；Culture basin, fluid reservoir, outer row's peristaltic pump, ultraviolet sterilization device, circulate peristaltic pump and converge the circulation loop that pond is in turn connected to form nutrition fluid circulation；Converge pond connection semi-conductor condensation dehydrating unit and nutrition liquid syringe；Culture basin and fluid reservoir are in base, the top connection translucent cover of Culture basin, and quartz sand is provided with Culture basin.The utility model can mark nitrogen and carbon isotope ratios simultaneously, and realize that it is recycled, without common12C and14N pollutes, and can obtain the nitrogen and carbon isotope ratios double labelling plant of high abundance.
Description
Technical field
The utility model is related to isotope labelling techniques field, more particularly to a kind of culture high abundance isotope carbon, nitrogen pair
The circulatory system of labeled plant sample.
Background technology
In recent years, contained by crop material the cyclic process of carbon, nitrogen in soil turns into Plant Nutrition, soil
One of study hotspot.Isotope tracer technique is to study crop material to decompose in soil and the crucial skill of conversion process
Art, can effectively disclose the release rule of stalk element and the biological effectiveness of organic nutrient.By stable isotope nitrogen (15N)
During the crop material of mark is manured into soil, by determining in soil different component15The change of N abundance, just can accurately calculate straw
Stalk15The quantity that N nutrients are shifted to soil, plant, and the ratio lost by approach such as gas, leachings.Equally, using stable
Property isotope carbon (13C) spike, with reference to modern molecular biology method, the series of stable that has been born isotope probe technology
(SIP), studying and describe the decomposition whereabouts of straw carbon, and the biological mistake of biochemical action synthesising biological macromolecular is passed through
Journey, so as to further disclose the microbiology mechanism of Straw decomposing.However, the conversion of straw carbon, nitrogen is one and connected each other
Cyclic process, the two occurs simultaneously, and transformation mechanism is closely connected, indivisible.Therefore, research straw carbon, nitrogen conversion process
Basis and premise be exactly isotope carbon, the nitrogen double labelling plant sample for obtaining high abundance.
Based on existing plant isotope labelling techniques are marked with single-element.Chinese invention patent
CN200610019742.4, CN201310513820.6 and utility model patent CN201420736248.X disclose one kind
Carbon dioxide isotope (13C-CO2) labelling apparatus, and using the plant of soil incubation as tagged object, be not related to and mark simultaneously
Two kinds of isotopes；Although patent of invention CN201410342182.0 discloses a kind of isotope stalk double labelling tracing method,
The method is mainly utilized13C- glucose and15N- urea is directly injected into plant, is mainly used in studying root exudates, this mark
Note method is not belonging to the normal assimilation category of plant, assimilates carbon dioxide by normal photosynthesis with plant and passes through
Normal Root Absorption assimilation nitrogen, which is compared, greatest differences.
Most of isotope labelling method is using soil as culture matrix, because soil contains substantial amounts of common carbon atom in itself
(12C), by Microorganism respiration, these carbon atoms can be with12C-CO2Form is largely discharged into air and is absorbed by plants profit
With causing labeled plant sample13C abundance is reduced.In research crop material decomposable process, low-abundance isotope is planted
Thing sample can not realize that (such as DNA level) carries out spike to carbon atom on a molecular scale；In addition, entering by culture matrix of soil
OK15When N is marked, substantial amounts of common nitrogen-atoms in soil (14N it) can also be absorbed by plants, cause plant15N abundance is too low.Cause
This, it is to obtain high abundance isotope carbon, the precondition of nitrogen double labelling plant sample to select appropriate training method.
Existing plant isotope labelling techniques are difficult to realize the continuous cycle labeling of whole plant growth period, cause same position
Element wastes serious, and mark cost is high, for example,13C-CO2It is substantial amounts of when confined space is timed opening during pulse labeling13C-CO2Release；And for example under the conditions of water planting in nutrient solution15N nutrients can not be recycled, substantial amounts of in old nutrient solution15N is abandoned
Waste.The technological difficulties of continuous cycle labeling are that the air humidity in closed environment can be continued to increase, and cause plant growth ring
Border deteriorates, and the disease aggravation such as mould, plant growth is obstructed, and causes plant isotope marks to fail；How stable isotope is realized
Carbon, the continuous cycle labeling of nitrogen are the key points for obtaining high abundance double labelling plant sample.
At present, this area does not provide above mentioned problem the solution of systematization.Planted however, obtaining high abundance double labelling
Thing sample is the necessary material for studying plant residue carbon, nitrogen decomposable process and mechanism.Therefore, need badly a kind of low cost, high efficiency,
The culture isotope carbon of integration, the circulatory system of nitrogen double labelling plant.
Utility model content
(1) technical problem to be solved
The utility model provides a kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample, is used for
Isotope carbon, the Cyclic culture of nitrogen double labelling can not be realized by solving existing isotope labelling techniques, it is impossible to obtain the double of high abundance
The problem of labeled plant sample.
(2) technical scheme
In order to solve the above-mentioned technical problem, the utility model provides a kind of culture high abundance isotope carbon, nitrogen double labelling and planted
The circulatory system of thing sample, including closed culturing room, CO2Syringe, gas balance device, nutrition liquid syringe and nutrient solution
The circulatory system；The closed culturing room includes the translucent cover on top and the base of bottom, and the base is connected with the translucent cover,
Semi-conductor condensation dehydrating unit is provided with the translucent cover, the translucent cover external seal connects the CO2Syringe and the gas
Body bascule；The nutrition fluid circulation is compacted including Culture basin, fluid reservoir, outer row's peristaltic pump, ultraviolet sterilization device, circulation
Dynamic pump and converge pond, the Culture basin, fluid reservoir, outer row's peristaltic pump, ultraviolet sterilization device, circulate peristaltic pump and converge pond
It is in turn connected to form circulation loop；The pond of converging is also connected with the semi-conductor condensation dehydrating unit and nutrient solution injection
Device；The Culture basin and the fluid reservoir are sequentially arranged in the base from top to bottom, and the top connection of the Culture basin is described
The vegetable seeds that vernalization is inserted in quartz sand, the quartz sand is provided with translucent cover, the Culture basin.
Wherein, the Culture basin is connected with the fluid reservoir by mozzle, and the first ball float liquid is provided with the fluid reservoir
Bit switch.
Wherein, the ultraviolet sterilization device includes being provided with uviol lamp and second in ultraviolet sterilization room, the ultraviolet sterilization room
Floating-ball level switch, the lower limit water level of second floating-ball level switch is higher than the bottom of the ultraviolet sterilization room.
Wherein, semi-conductor condensation dehydrating unit is included in casing, the casing provided with radiator, the semiconductor being sequentially connected
Cooling piece and condenser.
Wherein, fan is additionally provided with the casing, the fan is installed at the position of the radiator, the case
Air vent is opened up on the side wall of body.
Wherein, the bottom of the casing is provided with the funnel close to the condenser, and the casing is sequentially communicated the funnel
Converge pond with described.
Wherein, the translucent cover and the base are the cylindrical member that openend is provided with flange, the flange of the translucent cover
With the mutually corresponding connection of the flange of the base, silicagel pad is provided between the flange of the translucent cover and the flange of the base,
To realize being tightly connected for the translucent cover and the base.
Wherein, the gas balance device is included in gas balance tank, the gas balance tank provided with the work laterally set
The opening of the translucent cover is communicated with plug, the gas balance tank, the opening is located at the bottom of the piston.
Wherein, the liquid storage area is sealedly connected with waste liquid syringe.
Wherein, CO is provided with the translucent cover2Infrared sensor.
(3) beneficial effect
A kind of culture high abundance isotope carbon of the utility model offer, the circulatory system of nitrogen double labelling plant sample, phase
Than being had the characteristics that in prior art：
1st, culture high abundance isotope carbon of the present utility model, the circulatory system of nitrogen double labelling plant sample, by closed
The combination of culturing room, gas balance device and nutrition fluid circulation, is marked while realizing nitrogen and carbon isotope ratios,
Recycling for nitrogen isotope, and the system integral sealing can be realized, without common12C and14N pollution so that culture is obtained
Double labelling plant nitrogen and carbon isotope ratios abundance it is very high.
2nd, ultraviolet sterilization device is provided with nutrition fluid circulation, is enabled in nutrient solution15N is gone out by ultraviolet
Bacterium Posterior circle is utilized, and does not change forms of nitrogen, and the absorption of plant is not influenceed, is passed through15N- nutrient solutions are carried by the secondary utilization of plant
High isotope utilization rate, reduction flag cost；
3rd, culture matrix quartz sand is provided as in cultivation region, Soil Respiration during soil incubation is can solve the problem that
Produce12C-CO2And liquid absorption is a large amount of in water planting13C-CO2The problem of, so as to planting during avoiding soil incubation and water planting
The influence of thing isotope abundance reduction；
4th, by the semi-conductor condensation dehydrating unit in translucent cover, it can solve the problem that culturing room's humidity is excessive under confined conditions
The problem of；By the way that semi-conductor condensation dehydrating unit is connected with nutrition fluid circulation, it can enter by condensed water by converging pond
Enter the cultivation region of quartz sand is housed, it is possible to increase efficiency of water application, reduce the frequency of watering；By in semi-conductor condensation dehydrating unit
Fan, gas can be promoted to flow in closed culturing room, accelerate CO2Mix, improve crop utilization rate.
Brief description of the drawings
Culture high abundance isotope carbon that Fig. 1 provides for the utility model, the circulatory system of nitrogen double labelling plant sample
Composition schematic diagram；
The structure chart for the nutrition fluid circulation that Fig. 2 provides for the utility model；
The structure chart for the closed culturing room that Fig. 3 provides for the utility model；
In figure, 1：Closed culturing room；101：Translucent cover；102：Base；2：CO2Syringe；3：Gas balance device；301：
Gas balance tank；302：Piston；4：Nutrition liquid syringe；5：Semi-conductor condensation dehydrating unit；501：Casing；502：Radiator；
503：Semiconductor chilling plate；504：Condenser；505：Fan；6：Culture basin；7：Fluid reservoir；8：Outer row's peristaltic pump；9：It is ultraviolet to go out
Bacterium device；901：Ultraviolet sterilization room；902：Uviol lamp；10：Circulate peristaltic pump；11：Converge pond；12：Quartz sand；13：Plant；
14：Mozzle；15：First floating-ball level switch；16：Second floating-ball level switch；17：Funnel；18：Silicagel pad；19：Waste liquid is noted
Emitter；20：CO2Infrared sensor；21：Rubber stopper.
Embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings
State.Better embodiment of the present utility model is given in accompanying drawing.It these are only preferred embodiment of the present utility model, and unprovoked
This limits the scope of the claims of the present utility model, equivalent structure that every utilization the utility model specification and accompanying drawing content are made or
Equivalent flow conversion, or other related technical fields are directly or indirectly used in, similarly it is included in of the present utility model special
In sharp protection domain.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with belonging to technology of the present utility model
The implication that the technical staff in domain is generally understood that is identical.It is herein to be in term used in the description of the present utility model
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein " and/or " bag
Include the arbitrary and all combination of one or more related Listed Items.
The utility model provides a kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample, is used for
Isotope carbon, nitrogen double labelling can not be realized by solving existing isotope labelling techniques, it is impossible to realize carbon, the circulation training of nitrogen double labelling
Support, it is impossible to the problem of obtaining the double labelling plant sample of high abundance.
As Figure 1-3, a kind of culture high abundance isotope carbon, nitrogen double labelling plant are provided in the utility model embodiment
The circulatory system of sample, including closed culturing room 1, CO2Syringe 2, gas balance device 3, nutrition liquid syringe 4 and nutrition
Fluid circulation；Closed culturing room 1 includes the translucent cover 101 on top and the base 102 of bottom, the connection translucent cover of base 102
101, formed and semi-conductor condensation dehydrating unit 5 is provided with completely closed culturing room 1, translucent cover 101, in the culture of plant sample
During, it can be dehumidified at interval of a period of time using semi-conductor condensation dehydrating unit is interior to closed culturing room 1, translucent cover
101 external seals connect CO2Syringe 2 and gas bascule 3, CO2Syringe 2 can inject into closed culturing room 113C-
CO2, so that providing plant needs what is marked13C-CO2；Translucent cover 101 passes through the gas balance device 3 outside pipeline connection, energy
Enough ensure the continuous addition into closed culturing room 113C-CO2The atmospheric pressure in closed culturing room 1 keeps normal afterwards, and in closed training
The reduction of the temperature of room 1 is supported, when gas volume reduces, the gas in gas balance device 3 can be back to closed culturing room 1 in time
It is interior so that13C-CO2It can be fully utilized.In addition, nutrition fluid circulation includes Culture basin 6, fluid reservoir 7, outer row's peristaltic pump
8th, ultraviolet sterilization device 9, circulation peristaltic pump 10 and pond 11, Culture basin 6, fluid reservoir 7, outer row's peristaltic pump 8, ultraviolet sterilization are converged
Device 9, circulation peristaltic pump 10 and converge pond 11 and be in turn connected to form circulation loop；Converge pond 11 and be also connected with semi-conductor condensation and remove
Wet device 5 and nutrition liquid syringe 4；Culture basin 6 and fluid reservoir 7 are sequentially arranged in base 102 from top to bottom, the top of Culture basin 6
Portion, which connects to be provided with quartz sand 12, quartz sand 12 in translucent cover 101, Culture basin 6, is equipped with plant 13, sets and makees in Culture basin 6
For culture matrix quartz sand, Soil Respiration is produced during can solve the problem that soil incubation12C-CO2And liquid is inhaled in water planting
Receive a large amount of13C-CO2The problem of, so that the influence reduced during avoiding soil incubation and water planting to plant isotope abundance；Plant
Thing 13 is in the case where illumination passes through translucent cover 101, and the leaf of plant 13 absorbs CO by photosynthesis2Syringe 2 injects close
Close in culturing room 113C-CO2, so as to realize the mark of isotope carbon；The Root Absorption of plant 13 flows into culture by converging pond 11
In basin 615N- nutrient solutions, so as to realize the mark of isotope nitrogen；In nutrition fluid circulation, nutrition liquid syringe 4 can be to
Converge and new nutrient solution is injected in pond 11, so as to be provided to plant15N；15N- nutrient solutions enter Culture basin 6, culture by converging pond 11
It is excessive in basin 615N- nutrient solutions are flowed into fluid reservoir 7, when in fluid reservoir 715When N- nutrient solutions are excessive,15N- nutrient solutions are outside
Sterilized in the presence of row's peristaltic pump 8 into ultraviolet sterilization device 9, after sterilizing15N- nutrient solutions are in circulation peristaltic pump 10
Converge pond 11 under effect again, can solve the problem that by the nutrition fluid circulation can not frequently change nutrition under confined conditions
The problem of liquid, ultraviolet sterilization device 9 is provided with nutrition fluid circulation, is enabled in nutrient solution15N is gone out by ultraviolet
Bacterium Posterior circle is utilized, and does not change forms of nitrogen, and the absorption of plant is not influenceed, is passed through15N- nutrient solutions are carried by the secondary utilization of plant
High isotope utilization rate, reduction flag cost；Semi-conductor condensation dehydrating unit is imitated using the Peltier of semiconductor chilling plate
Should, hydrogenesis Cheng Shui in part in air is enabled to, condensed water converges pond 11 by pipeline, entered by converging pond 11
In Culture basin 6, water-vapour benign cycle in closed culturing room 1 is realized, air humidity in closed culturing room 1 can either be reduced, again
Condensed water can be made by the secondary utilization of plant, efficiency of water application is improved, reduce the frequency of watering in plant incubation, this is partly led
The dehumidification process of body dehumidification by condensation device belongs to physics dehumidifying, and other harmful chemicals, and small volume are not introduced, low in energy consumption,
Radiating is small, being capable of continuous firing.
Culture high abundance isotope carbon of the present utility model, the circulatory system of nitrogen double labelling plant sample, pass through closed training
The combination of room, gas balance device and nutrition fluid circulation is supported, is marked while realizing nitrogen and carbon isotope ratios, and
Recycling for nitrogen and carbon isotope ratios can be realized, the system integral sealing, without common12C and14N pollution so that culture is obtained
Double labelling plant nitrogen and carbon isotope ratios abundance it is very high.The Cyclic culture system effectiveness is high, easily operated, cost is low；Plant it is whole
Individual labeling process is completely closed, can realize continued labelling, prevent13C-CO2Leakage,15N- nutrient solutions can be recycled；Pass through
Semi-conductor condensation dehydrating unit can strictly control humidity, promote plant growth, reduction flag cost.
In the present embodiment, as shown in Fig. 2 Culture basin 6 is connected with fluid reservoir 7 by mozzle 14, provided with the in fluid reservoir 7
One floating-ball level switch 15.Specifically, the bottom of Culture basin 6 is connected with the top of fluid reservoir 7 by a diameter of 1cm mozzle 14,
Liquid Penetrant in quartz sand 12 enters fluid reservoir 7 to the bottom of Culture basin 6 by mozzle 14；The volume of fluid reservoir 7 is
1.5L, the body in the space being built-in between small-sized floating-ball level switch, i.e. the first floating-ball level switch 15, its upper and lower limit water level
Product is 1L；Fluid reservoir 7 is connected by drainpipe with external ultraviolet sterilization device 9, and drainpipe installs outer row's peristaltic pump 8 additional；Work as liquid storage
Liquid rises to upper limit water level in room 7, and the first floating-ball level switch 15 starts outer row's peristaltic pump 8 and worked, and outer row's peristaltic pump 8 sets stream
Speed is 50ml/min, and the liquid in fluid reservoir 7 is evacuated in ultraviolet sterilization device 9, when liquid is down to down and restricted water supply in fluid reservoir 7
Position, the first floating-ball level switch 15 closes outer row's peristaltic pump 8.
In the present embodiment, ultraviolet sterilization device 9 includes being provided with uviol lamp in ultraviolet sterilization room 901, ultraviolet sterilization room 901
902 and second floating-ball level switch 16, the lower limit water level b of the second floating-ball level switch 16 are higher than the bottom of ultraviolet sterilization room 901.
Specifically, ultraviolet sterilization room 901 is darkroom, built with uviol lamp 902, it is to be understood that uviol lamp 902 could be arranged to
Many, in the present embodiment, install 2 uviol lamps 902 in ultraviolet sterilization room 901 additional, its 253.7nm uitraviolet intensity radiated must not
Less than 70uW/cm2；Ultraviolet sterilization room 901 is built-in with small-sized floating-ball level switch, i.e. the second floating-ball level switch 16, and second floats
The volume in the space between upper and lower limit water level a, b of ball liquid-level switch 16 is 0.5L, is restricted water supply under the second floating-ball level switch 16
Spatial volume between position b and the bottom of ultraviolet sterilization room 902 is 0.5L；When liquid rises to upper limit water level a in ultraviolet sterilization room,
Two floating-ball level switch 16 start circulation peristaltic pump 10 and worked, by ultraviolet sterilization room 90115N- nutrient solutions, which are evacuated to, converges pond
11, finally return to the Culture basin 6 equipped with the culture matrix of quartz sand 12, when liquid is down to lower limit water level in ultraviolet sterilization room 901
B, the second floating-ball level switch 16 closes circulation peristaltic pump 10；The lower limit water level b and ultraviolet sterilization of second floating-ball level switch 16
Leave 0.5L space in the bottom of room 901, it can be ensured that there is liquid to be in sterilizing state in ultraviolet sterilization room 901 all the time.
In the present embodiment, semi-conductor condensation dehydrating unit 5 is included in casing 501, casing 501 provided with the radiating being sequentially connected
Device 502, semiconductor chilling plate 503 and condenser 504.
In the present embodiment, fan 505 is additionally provided with casing 501, fan 505 is installed at the position of radiator 502,
Air vent is opened up on the side wall of casing 501.
In the present embodiment, the bottom of casing 501 is provided with the funnel 17 close to condenser 504, and casing 501 is sequentially communicated funnel
17 and converge pond 11.
Pass through the Peltier effects of semiconductor chilling plate 503 so that close close to the side absorption refrigeration of condenser 504
The side heat release of radiator 502, when humid air is by condenser 504, the part hydrogenesis Cheng Shui in air, condensed water leads to
Cross funnel 17 and flow to and converge in pond 11, be back to by converging pond 11 in Culture basin 6, fan 505 is located at the one of radiator 502
Side, due to opening up air vent on the side wall of casing 501, in the presence of fan 505, can speed up the sky in closed culturing room 1
Flow of air so that the air humidity rapid decrease in closed culturing room 1；In addition, in the presence of fan 505, can speed up closed
Gas flows in culturing room 1, promotes plant pair13C-CO2Assimilation utilize, improve plant13C abundance.
The maximum moisture removal of semi-conductor condensation dehydrating unit 5 is 500ml/24h, and the working time is set as：At interval of 3 hours
Semi-conductor condensation dehydrating unit 5 works 1 hour；After continued labelling, the air humidity of closed culturing room 1 is up to 100%, fan
Start, drive the gas flowing in closed culturing room 1, humid air passes through the interior conduit of condenser 504, utilizes semiconductor chilling plate
Peltier effects so that hydrogenesis Cheng Shui in part in air, condensed water is back to equipped with stone by funnel 17 and drainpipe
The Culture basin 6 of the matrix of sand 12.
In the present embodiment, as shown in figure 3, forming closed closed culturing room after connection for the ease of translucent cover and base
1 and conveniently closed culturing room 1 is dismantled, conveniently insert Culture basin 6 and fluid reservoir 7, and conveniently cultivated in plant 13
Cheng Hou, takes out plant 13, and translucent cover 101 and base 102 are the cylindrical member that openend is provided with flange, the flange of translucent cover 101
It is attached after corresponding with the lugs position of base 102, and be provided between the flange of translucent cover 101 and the flange of base 102
Silicagel pad 18, ensure that the sealing of the flange of translucent cover 101 and the flange of base 102, the flange and base of translucent cover 101
Multiple screws are distributed on 102 flange, screw is passed through by bolt, the flange of translucent cover 101 and the flange of base 102 is realized
Connection.
Before being connected with, Culture basin 6 is put into base 102, the outer straight warp of Culture basin 6 is equal with the internal diameter of base 6；
Quartz sand 12 is put into Culture basin 6, quartz sand 12 pours into 0.5 times suddenly using 400 DEG C of high-temperature sterilization 4h are preceding needed after cooling
Glan nutrient solution makes its water content saturation, after the addition of quartz sand 12, and the upper edge of its top layer and Culture basin 6 is at a distance of 3cm；Stone
The vegetable seeds of vernalization is added in sand 12, burying depth is 2cm, is soaked 3 minutes using 95% ethanol before presprouting of seeds,
Then soaked 8 minutes, then rinsed 3 times using aqua sterilisa using 5% sodium hypochlorite.
In the present embodiment, gas balance device 3 is included in gas balance tank 301, gas balance tank 301 provided with laterally setting
Piston 302, the opening of translucent cover 101 is communicated with gas balance tank 301, opening is positioned at the bottom of piston 302.
Closed culturing room 1 by connect outside pipeline have in gas balance device 3, gas balance tank 301 can freely on move down
Dynamic piston 302, duct entry is located at the lower section of piston 302；The volume of gas balance tank 301 is more than 2L；When lasting to closed
Culturing room 1 adds13C-CO2Afterwards, its air pressure inside increase so that excessive gas in closed culturing room 1 is to gas balance device 3
Middle movement, piston 302 is moved up；Conversely, when the temperature reduction in closed culturing room 1 or plant absorption13C-CO2Draw etc. factor
Pressure drop of getting angry is low, and piston 302 is moved down, and the gas in gas balance device 3 is moved to closed culturing room 1, keeps closed training
Support the normal atmosphere (An) in room 1.
In the present embodiment, fluid reservoir 7 is sealedly connected with waste liquid syringe 19, during nutrient solution is recycled, and works as circulation
In nutrient solution15After N nutrients are gradually absorbed by plant 13, by the waste liquid syringe 19 that connects outside fluid reservoir 7 by discarded nutrient solution
Extracted out from fluid reservoir 7, and the nutrition liquid syringe 4 for converging pond 11 by connection adds new nutrient solution to equipped with quartz sand
The Culture basin 6 of 12 matrix.
In the present embodiment, CO is provided with translucent cover 1012Infrared sensor 20.After translucent cover 101 and base 102 are sealed, profit
With the CO of triple valve2Syringe 20 quantitatively injects into closed culturing room 113C-CO2, utilize CO2Infrared sensor 20 detects closed
CO in culturing room 12Concentration, the CO in closed culturing room 12Concentration maintains 300ppm-600ppm, it is ensured that plant 13 will not be luxurious
It is wasteful to absorb13C-CO2；Wherein, CO2The probe of infrared sensor 20 is positioned at the inside of closed culturing room 1, CO2Infrared sensor 20
Display location is popped one's head in the outside of closed culturing room 1 and the connecting line of both display screens passes through translucent cover 1 by rubber stopper 21,
CO2The detection range of infrared sensor 20 is 1-2000ppm, and precision is 1%.In addition, the material of translucent cover 101 can select it is transparent
Lucite, the material of base 102 can select PVC.
In the present embodiment, converge pond 11 and be arranged in translucent cover 101, and the connection of the circulatory system is connected by pipeline
Connect, such as：Nutrition fluid circulation is connected by pipeline, and semi-conductor condensation dehydrating unit 5 is connected with converging pond 11 by pipeline, thoroughly
Bright cover 101 is connected with gas balance device 3 by pipeline.For the seal of the closed culturing room 1 of strict guarantee, all syringes
It is connected with pipeline by syringe needle through rubber stopper 21 with the closed internal pipeline of culturing room 1.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model
Within the spirit and principle of utility model, any modification, equivalent substitution and improvements made etc. should be included in the utility model
Protection domain within.
Claims (10)
1. a kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample, it is characterised in that including closed training
Support room, CO2Syringe, gas balance device, nutrition liquid syringe and nutrition fluid circulation；The closed culturing room includes
The translucent cover on top and the base of bottom, and the base is connected with the translucent cover, and semiconductor cooling is provided with the translucent cover
Solidifying dehydrating unit, the translucent cover external seal connects the CO2Syringe and the gas balance device；The nutrient solution circulation
System includes Culture basin, fluid reservoir, outer row's peristaltic pump, ultraviolet sterilization device, circulates peristaltic pump and converge pond, the culture
Basin, fluid reservoir, outer row's peristaltic pump, ultraviolet sterilization device, circulate peristaltic pump and converge pond and be in turn connected to form circulation loop；Institute
State and converge pond and be also connected with the semi-conductor condensation dehydrating unit and the nutrition liquid syringe；The Culture basin and the fluid reservoir
It is sequentially arranged in from top to bottom in the base, the top of the Culture basin, which is connected, is provided with stone in the translucent cover, the Culture basin
The vegetable seeds of vernalization is inserted in sand, the quartz sand.
2. culture high abundance isotope carbon according to claim 1, the circulatory system of nitrogen double labelling plant sample, its feature
It is, the Culture basin is connected with the fluid reservoir by mozzle, the first floating-ball level switch is provided with the fluid reservoir.
3. culture high abundance isotope carbon according to claim 2, the circulatory system of nitrogen double labelling plant sample, its feature
It is, the ultraviolet sterilization device includes being provided with uviol lamp and the second Floating Ball Liquid Level in ultraviolet sterilization room, the ultraviolet sterilization room
Switch, the lower limit water level of second floating-ball level switch is higher than the bottom of the ultraviolet sterilization room.
4. culture high abundance isotope carbon according to claim 1, the circulatory system of nitrogen double labelling plant sample, its feature
Be, semi-conductor condensation dehydrating unit includes casing, in the casing provided with the radiator being sequentially connected, semiconductor chilling plate and
Condenser.
5. culture high abundance isotope carbon according to claim 4, the circulatory system of nitrogen double labelling plant sample, its feature
It is, fan is additionally provided with the casing, and the fan is installed at the position of the radiator, the side wall of the casing
On open up air vent.
6. culture high abundance isotope carbon according to claim 4, the circulatory system of nitrogen double labelling plant sample, its feature
It is, the bottom of the casing is provided with the funnel close to the condenser, and the top of the funnel is connected with the casing, described
The bottom of funnel is connected with the pond of converging.
7. culture high abundance isotope carbon according to claim 1, the circulatory system of nitrogen double labelling plant sample, its feature
It is, the translucent cover and the base are cylindrical member of the openend provided with flange, the flange of the translucent cover and the bottom
The flange of seat mutually corresponds to connection, silicagel pad is provided between the flange of the translucent cover and the flange of the base, to realize
The translucent cover is tightly connected with the base.
8. culture high abundance isotope carbon according to claim 1, the circulatory system of nitrogen double labelling plant sample, its feature
It is, the gas balance device is included in gas balance tank, the gas balance tank provided with the piston laterally set, the gas
The opening of the translucent cover is communicated with body compensator, the opening is located at the bottom of the piston.
9. culture high abundance isotope carbon according to claim 1, the circulatory system of nitrogen double labelling plant sample, its feature
It is, the liquid storage area is sealedly connected with waste liquid syringe.
10. culture high abundance isotope carbon according to claim 1, the circulatory system of nitrogen double labelling plant sample, it is special
Levy and be, CO is provided with the translucent cover2Infrared sensor.
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| CN201621485596.XU CN206365334U (en) | 2016-12-31 | 2016-12-31 | A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample |
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| CN201621485596.XU CN206365334U (en) | 2016-12-31 | 2016-12-31 | A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047265A (en) * | 2016-12-31 | 2017-08-18 | 中国农业科学院农业资源与农业区划研究所 | A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample |
| CN108093930A (en) * | 2017-12-12 | 2018-06-01 | 西北师范大学 | A kind of photosynthetic plant tracer case |
| CN108626613A (en) * | 2017-12-15 | 2018-10-09 | 杭州彬康农业科技有限公司 | A kind of energy saving laser plant lamp to be radiated using water cooling high-efficiency |
| CN111751500A (en) * | 2020-07-06 | 2020-10-09 | 吉林建筑大学 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
-
2016
- 2016-12-31 CN CN201621485596.XU patent/CN206365334U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047265A (en) * | 2016-12-31 | 2017-08-18 | 中国农业科学院农业资源与农业区划研究所 | A kind of culture high abundance isotope carbon, the circulatory system of nitrogen double labelling plant sample |
| CN108093930A (en) * | 2017-12-12 | 2018-06-01 | 西北师范大学 | A kind of photosynthetic plant tracer case |
| CN108626613A (en) * | 2017-12-15 | 2018-10-09 | 杭州彬康农业科技有限公司 | A kind of energy saving laser plant lamp to be radiated using water cooling high-efficiency |
| CN111751500A (en) * | 2020-07-06 | 2020-10-09 | 吉林建筑大学 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
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