CN101015248A - Method and device for plants carbon isotope labelling - Google Patents
Method and device for plants carbon isotope labelling Download PDFInfo
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- CN101015248A CN101015248A CNA2006100197424A CN200610019742A CN101015248A CN 101015248 A CN101015248 A CN 101015248A CN A2006100197424 A CNA2006100197424 A CN A2006100197424A CN 200610019742 A CN200610019742 A CN 200610019742A CN 101015248 A CN101015248 A CN 101015248A
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Abstract
The invention discloses a method and device for marking plant carbon isotopes. The method comprises following steps: sowing seed in the cultivating disk which located in the soil; putting cultivating disk into air- tight box; adding sodium carbonate marked with carbon-14 or carbon- 13 under air- tight condition, carrying out reaction with hydrochloric acid in reaction tank to generate carbon dioxide for plant photosynthesis. The carbon dioxide in box is mixed with electrical fan, and light is replenished with daylight lamp, the temperature and humidity are reduced by using cooling tube, and water is added into cultivating disk with fluid infusion bottle. The plant marked with carbon- 13 or carbon- 14 is got after cultivation. The invention is characterized by simple and safe operation, prevention of carbon marked with carbon isotopes escape, increased utilization coefficient of carbon isotopes, suitability for carbon isotopes mark for various plants, especially for woody plant.
Description
Technical field
The present invention relates to the isotope tracer technique field, more specifically relate to a kind of method of plants carbon isotope labelling, also relate to the device of plants carbon isotope labelling simultaneously.The researcher that the present invention is suitable for institution of higher learning and R﹠D institution carries out using in carbon-14 or carbon-13 tagging and the tracer study to plant.
Background technology
Isotope tracer technique is widely used in the research in each field of biological subject because the sensitivity of its height is called as the another big invention after the microscopy invention.In agrology research, the soil organic matter is not only the important material base of soil fertility, and for keeping soil aggregation structure and water conservation venting capability to have important function, the circulation of research soil organic carbon and the research of accumulation have great importance for preservation of fertility and agricultural sustainable development.The accumulation of the soil organic matter and decomposition and mineralising play crucial regulating and controlling effect for atmosphere " greenhouse gases " simultaneously, and studying the soil carbon circulation over nearly 20 years has become one of agrology hot issue.Adopting carbon-14 or carbon-13 tracer technique is that the research plant residue decomposes in soil and transformation power is learned and the key means of the organic carbon amount input soil of plant root exudation, for understanding soil organic matter circulation and accumulation and providing the parameter aspect to have important effect for setting up soil organic matter computer simulation model.And plants carbon isotope labelling and tracer technique are the bases of above-mentioned research.Owing to the carbonic acid gas of carbon-14 that has only gaseous state usually or labeling could be by plant absorbing and utilization, and two kinds of carbon isotope prices are all very expensive, when carrying out mark, should guarantee plant normal growth, the strict again airtight carbonic acid gas of carbon isotope labelling that prevents escapes, particularly because the carbonic acid gas of carbon-14 mark has radioactivity.Therefore, the method and apparatus of setting up suitable plants carbon isotope labelling is very important.
Nineteen sixties Britain pedologist Jenkinson has designed a kind of device of producing the isotope-labeled plant of carbon-14, but it is comparatively complicated, be not easy to operation, and there is defective in case internal pressure control method, when particularly when carrying out mark, adding the sodium bicarbonate of hydrochloric acid and carbon-14 mark, acutely be difficult to guarantee to prevent to leak because of reacting.This device not only easily grows nontarget organisms such as algae owing to adopt liquid cultivating method to carry out plant production in addition, and with soil in growing plants may there are differences some woody plant even be difficult in normal growth in the liquid medium; And this method is not suitable for studying the organic matter carbon amount of plant root exudation yet.Many researchers adopts intermittently tracer method usually at home, promptly adopt the potted plant plant of soil under the natural air environmental condition, to grow, every the regular period plant is moved into and to carry out carbon isotope labelling in the mark case one day, also there is the leakage of the carbon dioxide that is difficult to strict control mark in this method, be not suitable for studying plant root exudation organic carbon amount too, and the mark intensity heterogeneity of carbon isotope between material in the vegetable material of mark is not suitable for studying decomposition and the conversion of organic carbon in soil of labeled plant.
Summary of the invention
The objective of the invention is to be to provide a kind of method of plants carbon isotope labelling, this method is easy, easy operating, and practicality is wide, and plant is carried out carbon isotope labelling and studies root system of plant to soil input carbon amount.
Another object of the present invention is the device that is to provide a kind of carbon isotope labelling plant, this apparatus structure is simple, cost is low, be easy to make, use easy and simple to handlely, labeling process is airtight fully, and the carbon dioxide leakage of carbon-14 or labeling has been controlled in strictness, improve the availability of carbon-14 or carbon-13 isotope materials, adopted soil cultivation to be suitable for the carbon isotope labelling and the spike of each plant species.
To achieve these goals, the method for plants carbon isotope labelling of the present invention comprises the following steps:
A, in dish for cultivating, add the fresh soil that the 13-15 kilogram is crossed 2 millimeters aperture sieve, in soil, spray ammonium sulfate, sodium dihydrogen phosphate and potassium chloride nutrient solution, seeds germinated is seeded in the dish for cultivating soil, regulate the 55%-105% of soil moisture content according to floristics, make seedling growth 2-3 days at 23-27 ℃ to saturation moisture capacity.
B, dish for cultivating is placed on the mark case inner bottom plating, be coated with one deck high-temperature vacuum silicone grease, tighten all screws that pass tank wall and chamber door, airtight chamber door at chamber door and tank wall junction.Close the valve of casing left side absorption bottle two ends, open the valve of casing the right absorption bottle two ends, open vavuum pump and vacuumize, make in the casing vacuum arrive-0.01 to-0.02Mpa; Close the valve of absorption bottle two ends, close vavuum pump; Not variation of negative pressure on the piezometer shows that the casing seal is good after 24 hours.
C, slowly add the sodium carbonate solution of carbon-14 (or carbon-13) mark and hydrochloric acid solution in reactive tank in confined conditions, the carbonic acid gas that reaction produces carbon-14 (or carbon-13) mark carries out the photosynthesis growth for plant.
The carbonic acid gas that adopts the electric fan mixing to produce in D, the casing.Replenish illumination with fluorescent lamp.Adopt cooling water flow to reduce the temperature inside the box to 24-26 ℃, reduce the interior relative air humidity of casing to 65%-75% through cooling tube.By infusion bottle supplementing water or ammonium sulfate, sodium dihydrogen phosphate and potassium chloride nutrient solution in dish for cultivating.Reaction residue in the reactive tank is discharged outside the case by the raffinate discharge pipe, and condensed water is discharged outside the case by condensed water discharge pipe in the case.
When E, cultivation end, the carbonic acid gas employing vavuum pump of carbon-14 (or carbon-13) mark in the residual case is bled and is adsorbed in the sodium hydroxide solution when making it by the absorption bottle.Open chamber door then, intactly take out plant, after cleaning, complete, drying, obtain the plant of carbon-14 (or carbon-13) mark.
Realize the method for above-mentioned mark, must rely on device of the present invention just can finish, this device comprises: the casing that airtight polyethylene board is made places on the wooden tank tower.A round chamber door is respectively established on two limits about casing, and chamber door is fixed on the tank wall by 16-18 the screw that passes tank wall and chamber door.Case top inwall is installed 2-4 root fluorescent lamp, and the daylight lamp power source line passes tank wall to case, links to each other with power supply by switch again.Two limit inwalls are respectively installed an electric fan about casing, and the fan power line passes tank wall to case, are linking to each other with power supply by switch.Two limit inwalls are respectively installed the copper snake type cooling tube that a length overall is a 1-1.5 rice about casing, and cooling tube two ends pass tank wall, are connected with drainpipe with cooling water inlet pipe respectively by valve again.Place 3-4 dish for cultivating on the case inner bottom plating.A reactive tank is placed on the case inner bottom plating left side, and reaction tank bottom is installed a reaction residue discharge pipe, and the raffinate discharge pipe passes reaction cell wall and tank wall to case, is connected with valve again.The casing bottom of right side is installed a condensed water discharge pipe, and condensed water discharge pipe passes tank wall to case, is connected with valve again.3-4 the absorption bottle that sodium hydroxide solution is housed installed on the casing left side, connects with rubber tube between the absorption bottle; Absorption bottle one end connects valve by rubber tube, passes in tank wall and the case by rubber tube to be communicated with again, and an absorption bottle other end connects valve by rubber tube, is communicated with atmosphere again.The absorption bottle that 3-4 is equipped with sodium hydroxide solution is also installed on casing the right, also connect with rubber tube between the absorption bottle, absorption bottle one end connects valve by rubber tube, passes in tank wall and the case by rubber tube to be communicated with again, the absorption bottle other end connects valve by rubber tube, is connected with oilless vacuum pump again.The carbon acid solution infusion bottle of a hydrochloric acid infusion bottle and carbon-14 or labeling is fixed on the outer wall left side, casing front respectively, the infusion bottle lower end connects the plastic infusion pipe that a band is pressed threshold switch respectively, the woven hose lower end is passed tank wall respectively and is entered in the case, places reactive tank then.Outer wall the right, casing front is 3-4 infusion nutrition liquor bottle fixedly, and the infusion bottle lower end connects the plastic infusion pipe that a band is pressed threshold switch respectively, and the woven hose lower end is passed tank wall and entered in the case, places dish for cultivating again.A thermometer is installed on the casing left hand wall, and thermometer probe is passed tank wall and is placed air in the case.Bent-tube boiler piezometer of the outer installation of casing left hand wall, piezometer one end pass tank wall and are communicated with case is interior, and the other end places outside the case and is communicated with atmosphere, adds atoleine in the bend pipe piezometer.The whole box body strictness is airtight, passes all pipelines of tank wall and seals with glass cement on every side.
The present invention has the following advantages:
1. slowly add the sodium carbonate solution of carbon-14 (or carbon-13) mark and hydrochloric acid solution in confined conditions in reactive tank, can prevent that reaction is too violent, the carbon-14 of generation or the carbonic acid gas of labeling are without any escaping loss;
2. the interim plant of whole cultivation all grows in closed box, not only improved the utilization ratio of carbon-14 (or carbon-13) isotope raw material, and carbon isotope has good homogeneity in the plant of mark;
3. adopt soil cultivation than the production, particularly woody plant that are more suitable for liquid culture in each plant species, and growing plants is more near the plant of natural conditions cultivation:
4. adopt cooling tube not only can reduce the oven temperature, degree, and it is interior because the airtight high humility of bringing adopts fluorescent lamp to replenish illumination simultaneously to reduce case, so more helps plant normal growth;
5. in the case fan has been installed, air has been flowed guaranteed in the case in the air carbonic acid gas of carbon-14 (or carbon-13) mark even, made between plant the mark intensity of carbon isotope identical;
In the case by having adorned thermometer and piezometer, help understanding at any time the temperature inside the box and pressure and it regulated and control:
7. this apparatus structure is simple, cost is low, easy, the safety of method of operating;
8. practicality is wide, not only is suitable for directly plant being carried out carbon isotope labelling, and is suitable for the use of root system of plant to aspect researchs such as the carbon amount of soil input (secretion) and photosynthesis of plants.
Description of drawings
Fig. 1 is the plants carbon isotope labelling schematic representation of apparatus.
Embodiment
The method of plants carbon isotope labelling of the present invention comprises the following steps:
A, in dish for cultivating, add soil, get and be equivalent to lower fresh top layer (0-20 centimetre) soil of the oven-dried weight 40-60 kilogram content of organic matter, remove visible plant residue, after suitably air-dry, cross 2 millimeters aperture sieve, spray ammonium sulfate, sodium dihydrogen phosphate and potassium chloride mixed solution equably in soil, the nitrogen that applies, phosphorus and potassium amount are respectively 80,20 and 80 milligrams of/kilogram soil, it is in 45 * 45 * 15 centimetres the dish for cultivating that soil is sub-packed in length * wide * height, every dish dress soil 13-15 kilogram.Seeds such as paddy rice or corn elder generation water is cleaned, place the culture dish that is lined with filter paper, add 10 ml distilled waters, place 25 ℃ to cultivate 3-4 days down in culture dish, make seed sprouting.Planting seeds such as the paddy rice that germinateed or corn in dish for cultivating, are regulated the 55%-105% of soil moisture content to saturation moisture capacity according to floristics.Make seedling growth 2-3 days at 23-27 ℃.
B, dish for cultivating is placed the mark case, be coated with one deck high-temperature vacuum silicone grease, tighten 16-18 the screw that passes tank wall and chamber door, airtight chamber door at chamber door and tank wall junction.In the bent-tube boiler piezometer, add atoleine.In the absorption bottle at case two ends, add 200 milliliter of 2 molar sodium hydroxide solution.Close the valve at absorption bottle two ends, the case left side earlier, bleed to make in the case with vavuum pump and form-0.01 to-0.02Mpa vacuum, when piezometer bend pipe both sides atoleine liquid level differs to 3-5 centimetre, close the valve at absorption bottle two ends, the case left side, when piezometer bend pipe both sides atoleine liquid level difference does not have significant change after 24 hours, show that box sealing well can carry out next step plant marking operation.
C, add the sodium carbonate liquor 300-500 milliliter of carbon-14 (or carbon-13) mark in reactive tank by the sodium carbonate liquor infusion bottle, its concentration of sodium carbonate is 1 mole when adopting the carbon-14 mark, the carbon-14 radioactive intensity is the 0.15-0.25 millicurie, and is added with 3-4 in this sodium carbonate liquor and drips phenolphthalein indicator.Add 2.5 mole hydrochloride solution 300-500 milliliters in reactive tank by the hydrochloric acid infusion bottle again, valve slowly dripped off hydrochloric acid solution on the woven hose in 12-24 hour by regulating, and is violent to prevent reaction.When reactant liquor becomes redness, show that sodium carbonate reacts completely.The sodium carbonate and the hydrochloric acid solution that add 1-2 carbon-14 mark (or carbon-13) according to the plant growing situation weekly.
In D, the mark cultivation process, open the interior electric fan of case and make the gas concentration lwevel of carbon-14 mark in the gas even; Open cooling water and reduce the temperature inside the box and air humidity by spiral coil cooling tube, observation every day and record the temperature inside the box 1-2 time, the temperature inside the box keeps should be about 24-26 ℃; Open fluorescent lamp 12 hours to replenish illumination; Do wet degree according to soil in plant growing situation and the dish for cultivating, add ammonium sulfate, sodium dihydrogen phosphate and the potassium chloride mixotrophism liquid or the aqueous solution by the infusion nutrition liquor bottle; The waste liquid of complete reaction is discharged outside the case by opening reaction residue discharge pipe valve in the reactive tank, and the condensed water of assembling behind the water vapor condensation in the case is discharged outside the case by opening the condensed water discharge pipe valve.
E, common mark cultivation can reach needed phytomass in 60-90 days, finished cultivation this moment.Finishing the sodium carbonate liquor that cultivation stops to add carbon-14 (or carbon-13) mark the last week.Open the valve at absorption bottle two ends then, open vavuum pump carbon-14 (or carbon-13) the mark carbonic acid gas that remains in the interior gas of case is adsorbed onto in the sodium hydroxide solution by air-flow.Open the chamber door on mark case both sides again, take out dish for cultivating, add water logging and do not have soil in the dish for cultivating, soaked 4-8 hour.Take out plant gently from dish for cultivating soil then, should keep plant root complete as far as possible, water cleans residual soil on the plant carefully, drains the water on the plant.Place again baking oven through 80-105 ℃ completed 30 minutes after, under 50-60 ℃ of condition, dry, obtain the vegetable material of carbon-14 (or carbon-13) mark.The vegetable material of mark can be used for promptly that plant residue carbon transforms and the research of aspect such as circulation in soil after pulverize, measuring its carbon-14 (carbon-13) radioactive intensity (or demeanour).In addition, by measuring the organic carbon radioactive intensity (or demeanour) of carbon-14 (or carbon-13) mark of pedo relict in the dish for cultivating, can calculate the organic carbon amount of plant root exudation.(seeing the following form)
The rice biological amount and the radioactive intensity (table 1) thereof of mark case method cultivation carbon-14 mark
| Labeling method | Floristics | Plant part | Biomass dry weight (g) | Carbon content (%) | Carbon-14 radioactive intensity (DPM/mgC) |
| The present invention | Paddy rice | The cauline leaf root | 47.0 13.8 | 39.9 38.5 | 12700 13800 |
The reagent preparation
1, ammonium sulfate, sodium dihydrogen phosphate and potassium chloride mixed solution: 20.0g (NH
4)
2SO
4, 4.08g NaH
2PO
4, 8.0g KCl is dissolved in distilled water and is settled to 2L.
2, sodium hydroxide solution (2mol/L): 80.0 chemical pure NaOH are dissolved in distilled water and are settled to 1L.
3, hydrochloric acid solution (2mol/L): 167.0ml chemical pure concentrated hydrochloric acid (HCI, proportion are 1.19g/ml) is dissolved in distilled water and releases 1L.
4, the sodium carbonate liquor of carbon-14 mark: 1166.0g Na
2CO
3Be dissolved in 10L distilled water, add 5 millicurie C
14-Na
2CO
3, the sodium carbonate liquor of carbon-14 mark.
5, the sodium carbonate liquor of labeling: the 583.0g abundance is the C of 10Atom%
13-Na
2CO
3Be dissolved in 5L distilled water.
The device of method of the present invention as shown in Figure 1, casing 1 is made by thick 12 millimeters polyethylene board, its length * wide * height is 2.5-2.8 * 1.3-1.5 * 1.0-1.5 rice, casing 1 strict sealing, and casing 1 places, and long * wide * height is on the wooden tank tower 10 of 2.5-2.8 * 1.3-1.5 * 0.4-0.6 rice.Respectively establish a diameter on casing about the 1 two limit walls and be 40-50 centimetre round polyethylene board chamber door 2 (chamber door 2 or be ellipse or square or rectangular), chamber door 2 is fixed on the tank wall by 16-18 the screw that passes tank wall and chamber door 2.Case top inwall is installed the fluorescent lamp 3 of 40 watts of 2-4 roots, and fluorescent lamp 3 power lines pass tank wall to case, link to each other with power supply by switch again, fluorescent lamp 3 act as increase illumination.Casing about 1 two limit inwalls are respectively installed one 30-50 watt electric fan 4, and electric fan 4 power lines pass tank wall to case, link to each other with power supply by switch again, and the effect of electric fan 4 is a gas in the blending bin.Casing about 1 two limit inwalls are respectively installed the copper snake type cooling tube 5 that a length overall is a 1-1.5 rice, and cooling tube 5 two ends pass tank wall, are connected with cooling water pipe by valve again, and acting as of cooling tube 5 reduces the temperature inside the box and humidity.Place on the case inner bottom plating 3-4 long * wide * height is 45 * 45 * 15 centimetres a polyethylene dish for cultivating 6, be placed with soil in the dish for cultivating 6 and be used for cultivated plant.It is 25 * 15 * 45 centimetres polyethylene reactive tank 7 that a long * wide * height is also placed on the case inner bottom plating left side, is used for the sodium carbonate liquor of carbon-14 (carbon-13) mark and the carbonic acid gas that the hydrochloric acid solution reaction produces carbon-14 (carbon-13) mark, is made for the thing growth and utilizes.A glass reaction raffinate discharge pipe 8 is installed in reactive tank 7 bottoms, and raffinate discharge pipe 8 passes reactive tank 7 walls and tank wall to case, is connected with valve again, is used for the reaction residue discharge.Casing 1 bottom of right side is installed a glass condensed water discharge pipe 9, and condensed water discharge pipe 9 passes tank wall to case, is connected with valve again, is used for condensed water discharge in the case.3-4 400 milliliters absorption bottle 11 is installed on casing 1 left side, and the sodium hydroxide solution of 2 moles of 200-250 milliliters is housed in the absorption bottle 11; Connect with rubber tube between the absorption bottle 11, absorption bottle 11 1 ends connect valve by rubber tube, pass in tank wall and the case by rubber tube again and be communicated with, absorption bottle 11 other ends connect valve by rubber tube, be communicated with the carbon dioxide of remaining carbon-14 (carbon-13) mark in the respiratory box when effect of absorption bottle 11 mainly is the cultivation end again with atmosphere.The absorption bottle 11 that 3-4 is equipped with sodium hydroxide solution is installed on casing 1 the right equally, also connect between the absorption bottle 11 with rubber tube, absorption bottle 11 1 ends connect valve by rubber tube, pass in tank wall and the case by rubber tube again and be communicated with, absorption bottle 11 other ends connect valve by rubber tube, be connected with oilless vacuum pump 12, the effect of vavuum pump 12 is to vacuumize or the interior gas of case again.Two 500 milliliters infusion bottle 13,14 is fixed on the outer wall left side, casing front respectively, one is 2 moles hydrochloric acid solution infusion bottle 13, one is the sodium carbonate solution infusion bottle 14 of 1 mole carbon-14 (or carbon-13) mark, infusion bottle 13,14 lower ends connect the plastic infusion pipe 16 that a band is pressed threshold switch respectively, woven hose 16 lower ends are passed tank wall respectively and are entered in the case, place reactive tank 7 again, the sodium carbonate solution that is respectively applied for conveying hydrochloric acid solution and carbon-14 (or carbon-13) mark is to reactive tank 7.Outer wall the right, casing 1 front is 3-4 500 milliliters infusion bottle 15 fixedly, infusion bottle 15 lower ends connect the plastic infusion pipe 16 that a band is pressed threshold switch respectively, woven hose 16 lower ends are passed tank wall and are entered in the case, place dish for cultivating 6 again, are used for carrying n p k nutrition liquid or water to dish for cultivating 6.The thermometer 17 of a range 100 degree is installed on casing 1 left hand wall, and thermometer probe is passed tank wall and is placed air in the case, is used to observe the temperature inside the box.Height of installation is a 50-80 centimetre high glass bent-tube boiler piezometer 18 on casing 1 left hand wall, piezometer 18 1 ends pass tank wall and are communicated with case is interior, the other end places outside the case and is communicated with atmosphere, and dress atoleine in piezometer 18 bend pipes is used for the inspection box internal pressure and changes.All pass the pipeline place all with the glass cement sealing tank wall, to guarantee the strict sealing of casing.
Claims (4)
1, a kind of method of plants carbon isotope labelling, it is characterized in that: it comprises the following steps:
A, add fresh soil in dish for cultivating, spray ammonium sulfate, sodium dihydrogen phosphate and potassium chloride nutrient solution in soil, seeds germinated is seeded in the dish for cultivating soil, regulates soil moisture content, makes seedling growth 2-3 days at 23-27 ℃;
B, dish for cultivating is placed on the mark case inner bottom plating, be coated with one deck high-temperature vacuum silicone grease at chamber door and tank wall junction, tighten the screw that passes tank wall and chamber door, airtight chamber door is closed the valve that bottle two ends are adsorbed on the casing left side, opens the valve of casing the right absorption bottle two ends, the unlatching vavuum pump vacuumizes, make in the casing vacuum arrive-0.01 to-0.02Mpa, close the valve of absorption bottle two ends, close vavuum pump;
C, add the sodium carbonate solution of carbon-14 or labeling and hydrochloric acid solution in reactive tank in confined conditions, the carbonic acid gas that reaction produces the carbon-14 mark carries out the photosynthesis growth for plant;
The carbonic acid gas that adopts the electric fan mixing to produce in D, the casing, replenish illumination with fluorescent lamp, adopt cooling water flow to reduce the temperature inside the box to 24-26 ℃ through cooling tube, reduce the interior relative air humidity of casing to 65%-75%, by infusion bottle supplementing water or ammonium sulfate, sodium dihydrogen phosphate and potassium chloride nutrient solution in dish for cultivating, reaction residue in the reactive tank is discharged outside the case by the raffinate discharge pipe, and condensed water is discharged outside the case by condensed water discharge pipe in the case;
E, when cultivation finishes, the carbonic acid gas of the carbon-14 mark in the residual case was adsorbed in the sodium hydroxide solution when adopting vavuum pump to bleed to make it by the absorption bottle, opened chamber door then, took out plant, obtained the plant of carbon-14 mark after cleaning, complete, drying.
2, a kind of device that is used to realize the described plants carbon isotope labelling method of claim 1 is characterized in that:
Respectively establish a chamber door (2) about casing (1), the screw that chamber door (2) passes tank wall and chamber door (2) is fixed on the tank wall, case top inwall is installed fluorescent lamp (3), casing (1) two limit inwall is respectively installed an electric fan (4), casing (1) two limit inwall is respectively installed copper snake type cooling tube (5), case inner bottom plating placing response groove (7), reaction residue discharge pipe (8) is installed in reactive tank (7) bottom, raffinate discharge pipe (8) passes reactive tank (7) wall and tank wall to case, be connected with valve again, condensed water discharge pipe (9) is installed in casing (1) bottom, condensed water discharge pipe (9) passes tank wall to case, be connected with valve again, the absorption bottle (11) that fills sodium hydroxide solution is installed on casing (1) left side, connect with rubber tube between the absorption bottle (11), absorption bottle (11) connects valve by rubber tube, pass in tank wall and the case by rubber tube again and be communicated with, absorption bottle (11) other end connects valve by rubber tube, be communicated with atmosphere again, the absorption bottle (11) that fills sodium hydroxide solution is installed on casing (1) the right, casing (1) outer wall is the fixing carbon acid solution infusion bottle (14) of hydrochloric acid infusion bottle (13) and carbon-14 or labeling respectively, infusion bottle (13,14) lower end connects the plastic infusion pipe (16) that a band is pressed threshold switch respectively, woven hose (16) lower end is passed tank wall respectively and is entered in the case, place reactive tank (7) then, casing (1) outer wall is infusion bottle (15) fixedly, infusion bottle (15) lower end connects the plastic infusion pipe (16) that a band is pressed threshold switch respectively, woven hose (16) lower end is passed tank wall and is entered in the case, places dish for cultivating (6) again.
3, the device of a plant species tagging method according to claim 2, it is characterized in that: connect with rubber tube between the absorption bottle (11), absorption bottle (11) one ends connect valve by rubber tube, pass in tank wall and the case by rubber tube again and be communicated with, adsorption tube (11) other end connects valve by rubber tube, is connected with vavuum pump (12) again.
4, the device of a plant species tagging method according to claim 2 is characterized in that: casing (1) is gone up dress thermometer (17), piezometer (18).
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