CN103698476A - Isotopic tracer method for determining carbon sources and carbon sinks of perennial vegetation in situ - Google Patents
Isotopic tracer method for determining carbon sources and carbon sinks of perennial vegetation in situ Download PDFInfo
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Abstract
The invention discloses an isotopic tracer method for determining carbon sources and carbon sinks of perennial vegetation in situ. The isotopic tracer method is characterized by comprising the following steps: (1) determining the concentration of carbon dioxide in local air, and marking the concentration as CO; (2) collecting samples (G1, G2 and G3) of a plant-covered land; (3) collecting device gas recovery performance samples (G4 and G5); (4) collecting a local gas carbon dioxide sample (G6); (5) carrying out the steps (1 to 4) in the middle of each month; (6) calculating the carbon sources and the carbon sinks for the CO, the G1, the G2, the G3, the G4, the G5 and the G6, which are obtained in the steps, to obtain the annual breathing carbon amount (A(year), ton) of vegetation per hectare, the annual net fixed carbon amount (B(year), ton) of the vegetation per hectare and the annual fixed carbon amount (D(year), ton) of the vegetation per hectare. The isotopic tracer method has the beneficial effects of high accuracy and simplicity in operation.
Description
Technical field
The invention belongs to environmental science and technology technical field, relate to the tracer method that the perennial vegetation carbon source and carbon of a kind of in-site detecting is converged.
Background technology
Mankind's activity has caused atmospheric carbon dioxide concentration sharply to increase, and the greenhouse effect that produce and whole world change have caused showing great attention to of various countries, the whole world.The estimation that carbon source and carbon is converged has become adaptation Global climate change and increasing remittance reduces discharging the important content in Mechanism Study.Chinese scholars has been done quantitative research for carbon remittance carbon source and evaluation method thereof.Wherein terrestrial ecosystems Carbon balance is one of key problem in whole world change science, has also set up the research method of many terrestrial ecosystems Carbon balance.Ground carbon isotope method is to analyze one of very useful method of Terrestrial Carbon balance, is specially adapted to inquire into dominant factor and the response to whole world change thereof of Terrestrial Carbon balance.Yet due to the complicacy of terrestrial ecosystems, ground isotope method is extrapolated to even water globe flat of region by result of study among a small circle, can cause larger error.Simultaneously ground isotope method is used microcell labelling method more, and the microcell that particular area is set carries out mark, and plant, soil are carried out adding a calculating after sampling and measuring, and operation easier is larger, is unsuitable for many microcells are set measure, and is especially not suitable for Various Seasonal and measures.
Summary of the invention
Object of the present invention is at the tracer method that is providing the perennial vegetation carbon source and carbon of a kind of in-site detecting to converge, and solved existing carbon source and carbon and converged the problem of the large and complicated operation of the error of the ground isotope method measured.
Technical scheme of the present invention is to carry out according to following steps:
Step 1: measure local air gas concentration lwevel and be designated as C0 (mg/L);
Step 2: the measurement operation to vegetal cover plot:
1. select 5, representational vegetal cover plot to test, every ground covers bottomless organic glass case, each glass box is carried out to following same operation: along chest bottom ditching, make chest base embed soil 5cm, with fine earth, at the bottom of case, cover again, compacting, opens all air intake openings, gas outlet, balance 15 minutes, 2. in the gas reaction bottle in gas generating unit, add the Na of 99% abundance, 1mg
2 13cO
3 solid sample 1, by Na
2 13cO
3 solid sample 1 is designated as G1, with emulsion tube, bottomless one of them air intake opening of organic glass case is connected with gas generating unit, close other three air intake openings and all gas outlet, with syringe, to the gas reaction bottle in gas generating unit, inject 0.1N hydrochloric acid solution 5ml, after a few minutes, by funnel external in gas generating unit, make gas reaction bottle fill with water, air is all discharged, close the air intake opening switch that connects gas generating unit, withdraw gas generating unit, the water of the gas reaction bottle in turned letter gas generating unit, the standing 2h of whole device, make the vegetation in bottomless organic glass case carry out photosynthesis, 3. in the gas receiving bottle in four gas absorbing devices, respectively add 100g monoethanolamine, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, with sebific duct, four vacuum pumps are connected with four gas absorbing devices, 4. start vacuum pump, open all gas outlets switch, carry out gas recovery, after 1 minute, open all air intake opening switches, after about 15 minutes, close all air intake openings, gas outlet switch, close vacuum pump, stop reclaiming, 5. every morning repeat 2. above-mentioned and 4. each twice, time is respectively 6:00-8:00 and 8:30-10:30, repeat afternoon 2. and 4. each twice, time is respectively 13:00-15:00 and 15:30-17:30, in that afternoon, 18:00 opens vacuum pump to the next morning during 6:00, and opens all air intake openings of bottomless organic glass case, gas outlet, continues receiver gases, withdraw gas absorbing device after 6:00 morning, and now in all gas receiving bottle, liquid biased sample is designated as G2, 6. all gas absorption plant is changed new receiving bottle and receiving liquid, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, start vacuum pump, open all air intake openings, gas outlet, until the 3rd day morning 6:00, withdraw all gas absorption plant, in all gas receiving bottle, liquid biased sample is designated as G3, withdraws all devices,
Step 3: device gas recyclability is identified:
1) at one, carry out on without plant level land, cover bottomless organic glass case, with fine earth, along base bedding compacting, air intake opening connects gas generating unit with sebific duct, and gas outlet connects gas receiving bottle, opens all air inlets, the entrance of giving vent to anger, balance 15 minutes; 2) in the gas reaction bottle in gas generating unit, add 99% abundance, the Na of 1mg
2 13cO
3solid sample 2, Na now
2 13cO
3solid sample 2 is designated as G4, with emulsion tube, bottomless one of them air intake opening of organic glass case is connected with gas generating unit, close other three air intake opening switches and all gas outlets switch, with syringe, to the gas reaction bottle in gas generating unit, inject 0.1N hydrochloric acid solution 5ml, after a few minutes, by funnel external in gas generating unit, make gas reaction bottle fill with water, air is all discharged, close the air intake opening that connects gas generating unit, withdraw gas generating unit, the water of the gas reaction bottle in turned letter gas generating unit, the standing 2h of whole device; 3) in the gas receiving bottle in four gas absorbing devices, respectively add 100g monoethanolamine, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, with sebific duct, four vacuum pumps are connected with four gas absorbing devices; 4) start vacuum pump, open all gas outlets, carry out gas recovery, after 1 minute, open all air intake openings, after about 15 minutes, close all air intake openings, gas outlet, close vacuum pump, stop reclaiming; 5) every morning repeats above-mentioned 2) and 4) each 2 times, the time is respectively 6:00-8:00 and 8:30-10:30, repeats 2 afternoon) and 4) respectively twice, the time is respectively 13:00-15:00 and 15:30-17:30; In that afternoon, during 18:00-6:00 the next morning, open vacuum pump, and open all air intake openings of bottomless organic glass case, gas outlet, continue receiver gases, withdraw gas absorbing device after 6:00 morning, and now in all gas receiving bottle, liquid biased sample is designated as G5;
Step 4: local atmospheric carbon dioxide is collected:
At one, carry out on without plant level land, cover bottomless organic glass case, with fine earth along base bedding compacting, air intake opening connects gas generating unit with sebific duct, gas outlet connects gas receiving bottle, open all air inlets, the entrance of giving vent to anger, balance 15 minutes, in gas absorbing device, in gas receiving bottle, add 100ml monoethanolamine, with emulsion tube, connect gas outlet and gas absorbing device, with sebific duct, vacuum pump is connected with gas absorbing device, open gas outlet switch, start vacuum pump, carry out gas recovery, after about 15 minutes, stop reclaiming, in gas receiving bottle, fluid sample is designated as G6,
Step 5: step 1 to step 4 every middle of a month is carried out once, experiment is carried out 1 year;
Step 6: carry out carbon source and carbon remittance and calculate:
Sample G1, G2, G3, G4, G5 and G6 measure respectively concentration of carbon, and prior art and standard for assay method are calculated total carbon, are designated as respectively C1, C2, C3, C4, C5 and C6, and unit is mg;
Sample G1, G2, G3, G4, G5 and G6 measure respectively on isotope ratio mass spectrometer
13c abundance, is designated as respectively α 1, α 2, α 3, α 4, α 5 and α 6;
Carbon source and carbon remittance computing method:
Device gas reclaiming rate calculates:
β(％)＝C5(α5-α6)/C4(α4-α6)×100
Spike in single test
13c amount:
χ1(mg)＝C1(α1-α6)，
Every m once
2vegetation first day reclaims spike
13c amount:
χ2(mg)＝C2(α2-α6)/β，
Experiment second day does not have
13c mark, the 13C of collection breathes to produce, one day every m
2vegetation breathes and produces spike
13c amount:
χ3(mg)＝C3(α3-α6)/β，
Suppose
12c and
13c photosynthetic fixing and breathe in there is no selectivity, every mg spike
13the total carbon of C representative is:
φ(mg)＝1000C0/χ1，
Wherein 1000 is the volume L of organic glass case, and C0 is atmosphere concentration of carbon mg/L,
Experiment second day does not have
13c mark, the 13C of collection breathes to produce, one day every m
2vegetation breathes carbon amount:
A＝φ×χ3，
Experiment first day photosynthesis of plant has been fixed
13c, plant respiration discharges again simultaneously
13c, spike in each test
13 c amount χ 1 and each every m
2vegetation first day reclaims spike
13the difference of C amount χ 2 is exactly one day every m
2vegetation is fixed only
13c amount, one day every m
2the clean fixing carbon amount of vegetation:
B＝φ×(χ1-χ2)，
One day every m
2the fixing carbon amount of plant photosynthesis is one day every m
2the clean fixing one day every m of carbon amount of vegetation
2vegetation breathes carbon amount sum:
D＝φ×(χ1-χ2)+φ×χ3
Monthly once test, within 1 year 12 months, carry out 12 times, average every day every m
2vegetation breathes carbon amount (A
on average, mg), average every day every m
2the clean fixing carbon amount (B of vegetation
flat all, mg) with average every day of every m
2carbon amount (the D that plant photosynthesis is fixing
on average, mg) be respectively:
A
on average=(∑ A)/12/5,
B
on average=(∑ B)/12/5,
D
on average=(∑ D)/12/5,
Annual 365 days, per hectare was 10000m
2, breathe carbon amount (A per hectare vegetation year
year, ton), per hectare vegetation year clean fixed carbon amount (B
year, ton) and per hectare vegetation year fixed carbon amount (D
year, ton) be respectively:
A
year=365 * 10
-9a
on average(ton/hectare),
B
year=365 * 10
-9b
on average(ton/hectare),
D
year=365 * 10
-9b
on average(ton/hectare).
The invention has the beneficial effects as follows that assay method precision height is simple to operate.
Accompanying drawing explanation
Fig. 1 is the structural representation of the bottomless organic glass case of experimental provision of the present invention;
Fig. 2 is the structural representation of experimental provision gas absorbing device of the present invention;
Fig. 3 is the structural representation of experimental provision gas generating unit of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
One, experimental provision:
Bottomless organic glass case (1m * 1m * 1m as shown in Figure 1, be that length and width height is all 1m), four jiaos, top is respectively equipped with 4 air intakes and 4 gas outlets from four jiaos of top margin 10cm, bottom from 10cm place, base, air intake is connected with the external world with the equal belt switch in gas outlet available sebific duct.
Gas absorbing device, as shown in Figure 2, inside has 1 gas receiving bottle 1 and the drying tower 2 that fills sodium hydrate solid, and gas receiving bottle 1 bottleneck stretches out and connects root pipe, a connection drying tower 2, and another root is used for connecing bottomless organic glass case gas outlet.
Gas generating unit, as shown in Figure 3, with the gas reaction bottle 4 of cork, two cuvettes pass in cork central authorities, a connection funnel, one is used for connecing bottomless organic glass case air intake opening.
Agents useful for same:
Na
2 13CO
3
Hydrochloric acid solution (0.1N)
Monoethanolamine, analyzes pure
Distilled water
Two, test method:
Step 1: local air gas concentration lwevel (CO2) is measured:
Utilizing conventional atmospheric carbon dioxide detector to measure all can.Conversion, for concentration of carbon, is designated as C0 (mg/L).
Step 2: the measurement operation to vegetal cover plot:
1. select 5, representational vegetal cover plot to test, every ground covers bottomless organic glass case, each glass box is carried out to following same operation: along chest bottom ditching, make chest base embed soil 5cm, with fine earth, at the bottom of case, cover again, compacting, opens all air intake openings, gas outlet, balance 15 minutes, 2. in the gas reaction bottle 4 in gas generating unit, add the Na of 99% abundance, 1mg
2 13cO
3 solid sample 1, by Na
2 13cO
3 solid sample 1 is designated as G1, with emulsion tube, bottomless one of them air intake opening of organic glass case is connected with gas generating unit, close other three air intake openings and all gas outlet, with syringe, to the gas reaction bottle 4 in gas generating unit, inject 0.1N hydrochloric acid solution 5ml, after a few minutes, by funnel external in gas generating unit, make gas reaction bottle 4 fill with water, air is all discharged, close the air intake opening switch that connects gas generating unit, withdraw gas generating unit, the water of the gas reaction bottle 4 in turned letter gas generating unit, the standing 2h of whole device, make the vegetation in bottomless organic glass case carry out photosynthesis, 3. in the gas receiving bottle 1 in four gas absorbing devices, respectively add 100g monoethanolamine, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, with sebific duct, four vacuum pumps 3 are connected with four gas absorbing devices, 4. start vacuum pump 3, open all gas outlets switch, carry out gas recovery, after 1 minute, open all air intake opening switches, after about 15 minutes, close all air intake openings, gas outlet switch, close vacuum pump 3, stop reclaiming, 5. every morning repeat 2. above-mentioned and 4. each twice, time is respectively 6:00-8:00 and 8:30-10:30, repeat afternoon 2. and 4. each twice, time is respectively 13:00-15:00 and 15:30-17:30, in that afternoon, 18:00 opens vacuum pump 3 during 6:00 to the next morning, and opens all air intake openings of bottomless organic glass case, gas outlet, continues receiver gases, withdraw gas absorbing device after 6:00 morning, and now in all gas receiving bottle 1, liquid biased sample is designated as G2, 6. all gas absorption plant is changed new receiving bottle and receiving liquid, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, start vacuum pump 3, open all air intake openings, gas outlet, until the 3rd day morning 6:00, withdraw all gas absorption plant, in all gas receiving bottle 1, liquid biased sample is designated as G3, withdraws all devices,
Step 3: device gas recyclability is identified:
1) at one, carry out on without plant level land, cover bottomless organic glass case, with fine earth, along base bedding compacting, air intake opening connects gas generating unit with sebific duct, and gas outlet connects gas receiving bottle 1, opens all air inlets, the entrance of giving vent to anger, balance 15 minutes; 2) in the gas reaction bottle 4 in gas generating unit, add 99% abundance, the Na of 1mg
2 13cO
3solid sample 2, Na now
2 13cO
3solid sample 2 is designated as G4, with emulsion tube, bottomless one of them air intake opening of organic glass case is connected with gas generating unit, close other three air intake opening switches and all gas outlets switch, with syringe, to the gas reaction bottle 4 in gas generating unit, inject 0.1N hydrochloric acid solution 5ml, after a few minutes, by funnel external in gas generating unit, make gas reaction bottle 4 fill with water, air is all discharged, close the air intake opening that connects gas generating unit, withdraw gas generating unit, the water of the gas reaction bottle 4 in turned letter gas generating unit, the standing 2h of whole device; 3) in the gas receiving bottle 1 in four gas absorbing devices, respectively add 100g monoethanolamine, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, with sebific duct, four vacuum pumps 3 are connected with four gas absorbing devices; 4) start vacuum pump 3, open all gas outlets, carry out gas recovery, after 1 minute, open all air intake openings, after about 15 minutes, close all air intake openings, gas outlet, close vacuum pump 3, stop reclaiming; 5) every morning repeats above-mentioned 2) and 4) each 2 times, the time is respectively 6:00-8:00 and 8:30-10:30, repeats 2 afternoon) and 4) respectively twice, the time is respectively 13:00-15:00 and 15:30-17:30; In that afternoon, during 18:00-6:00 the next morning, open vacuum pump 3, and open all air intake openings of bottomless organic glass case, gas outlet, continue receiver gases, withdraw gas absorbing device after 6:00 morning, and now in all gas receiving bottle 1, liquid biased sample is designated as G5;
Step 4: local atmospheric carbon dioxide is collected:
At one, carry out on without plant level land, cover bottomless organic glass case, with fine earth along base bedding compacting, air intake opening connects gas generating unit with sebific duct, gas outlet connects gas receiving bottle 1, open all air inlets, the entrance of giving vent to anger, balance 15 minutes, in gas absorbing device, in gas receiving bottle 1, add 100ml monoethanolamine, with emulsion tube, connect gas outlet and gas absorbing device, with sebific duct, vacuum pump 3 is connected with gas absorbing device, open gas outlet switch, start vacuum pump 3, carry out gas recovery, after about 15 minutes, stop reclaiming, in gas receiving bottle 1, fluid sample is designated as G6,
Step 5: step 1 to step 4 every middle of a month is carried out once, experiment is carried out 1 year;
Step 6: carry out carbon source and carbon remittance and calculate:
Sample G1, G2, G3, G4, G5 and G6 measure respectively concentration of carbon, and prior art and standard for assay method are calculated total carbon, are designated as respectively C1, C2, C3, C4, C5 and C6, and unit is mg;
Sample G1, G2, G3, G4, G5 and G6 measure respectively on isotope ratio mass spectrometer
13c abundance, is designated as respectively α 1, α 2, α 3, α 4, α 5 and α 6, and unit is mg; Carbon source and carbon remittance computing method:
Device gas reclaiming rate calculates:
β(％)＝C5(α5-α6)/C4(α4-α6)×100
Spike in single test
13c amount:
χ1(mg)＝C1(α1-α6)，
Every m once
2vegetation first day reclaims spike
13c amount:
χ2(mg)＝C2(α2-α6)/β，
Experiment second day does not have 13C mark, collection
13c breathes to produce, one day every m
2vegetation breathes and produces spike
13c amount:
χ3(mg)＝C3(α3-α6)/β，
Suppose
12c and
13c photosynthetic fixing and breathe in there is no selectivity, every mg spike
13the total carbon of C representative is:
φ(mg)＝1000C0/χ1，
Wherein 1000 is the volume L of organic glass case, and C0 is atmosphere concentration of carbon mg/L,
Experiment second day does not have
13c mark, collection
13c breathes to produce, one day every m
2vegetation breathes carbon amount:
A＝φ×χ3，
Experiment first day photosynthesis of plant has been fixed
13c, plant respiration discharges again simultaneously
13c, spike in each test
13 c amount χ 1 and each every m
2vegetation first day reclaims spike
13the difference of C amount χ 2 is exactly one day every m
2vegetation is fixed only
13c amount, one day every m
2the clean fixing carbon amount of vegetation:
B＝φ×(χ1-χ2)，
One day every m
2the fixing carbon amount of plant photosynthesis is one day every m
2the clean fixing one day every m of carbon amount of vegetation
2vegetation breathes carbon amount sum:
D＝φ×(χ1-χ2)+φ×χ3
Monthly once test, within 1 year 12 months, carry out 12 times, average every day every m
2vegetation breathes carbon amount (A
on average, mg), average every day every m
2the clean fixing carbon amount (B of vegetation
flat all, mg) with the average fixing carbon amount (D of every m2 plant photosynthesis every day
on average, mg) be respectively:
A
on average=(∑ A)/12/5,
B
on average=(∑ B)/12/5,
D
on average=(∑ D)/12/5,
Annual 365 days, per hectare was 10000m
2, breathe carbon amount (A per hectare vegetation year
year, ton), per hectare vegetation year clean fixed carbon amount (B
year, ton) and per hectare vegetation year fixed carbon amount (D
year, ton) be respectively:
A
year=365 * 10
-9a
on average(ton/hectare),
B
year=365 * 10
-9b
on average(ton/hectare),
D
year=365 * 10
-9b
on average(ton/hectare).
Method of the present invention adopts specific labelling apparatus to carry out original position mark, do not need plant and soil sample, simple to operate, more microcell can be set simultaneously, and according to carrying out different seasons, representativeness is stronger, and result of study is extrapolated to the carbon source and carbon of the whole terrestrial ecosystems of calculating and converges more reliable.
Claims (1)
1. the tracer method that the perennial vegetation carbon source and carbon of in-site detecting is converged, is characterized in that carrying out according to following steps:
Step 1: measure local air gas concentration lwevel and be designated as C0 (mg/L);
Step 2: the measurement operation to vegetal cover plot:
1. select 5, representational vegetal cover plot to test, every ground covers bottomless organic glass case, each glass box is carried out to following same operation: along chest bottom ditching, make chest base embed soil 5cm, with fine earth, at the bottom of case, cover again, compacting, opens all air intake openings, gas outlet, balance 15 minutes, 2. in the gas reaction bottle (4) in gas generating unit, add the Na of 99% abundance, 1mg
2 13cO
3solid sample 1, by Na
2 13cO
3solid sample 1 is designated as G1, with emulsion tube, bottomless one of them air intake opening of organic glass case is connected with gas generating unit, close other three air intake openings and all gas outlet, with syringe, to the gas reaction bottle (4) in gas generating unit, inject 0.1N hydrochloric acid solution 5ml, after a few minutes, by funnel external in gas generating unit, make gas reaction bottle (4) fill with water, air is all discharged, close the air intake opening switch that connects gas generating unit, withdraw gas generating unit, the water of the gas reaction bottle (4) in turned letter gas generating unit, the standing 2h of whole device, make the vegetation in bottomless organic glass case carry out photosynthesis, 3. in the gas receiving bottle (1) in four gas absorbing devices, respectively add 100g monoethanolamine, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, with sebific duct, four vacuum pumps (3) are connected with four gas absorbing devices, 4. start vacuum pump (3), open all gas outlets switch, carry out gas recovery, after 1 minute, open all air intake opening switches, after about 15 minutes, close all air intake openings, gas outlet switch, close vacuum pump (3), stop reclaiming, 5. every morning repeat 2. above-mentioned and 4. each twice, time is respectively 6:00-8:00 and 8:30-10:30, repeat afternoon 2. and 4. each twice, time is respectively 13:00-15:00 and 15:30-17:30, in that afternoon, 18:00 opens vacuum pump (3) during 6:00 to the next morning, and open all air intake openings of bottomless organic glass case, gas outlet, continue receiver gases, withdraw gas absorbing device morning after 6:00, now in all gas receiving bottle (1), liquid biased sample is designated as G2, 6. all gas absorption plant is changed new receiving bottle and receiving liquid, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, start vacuum pump (3), open all air intake openings, gas outlet, until the 3rd day morning 6:00, withdraw all gas absorption plant, in all gas receiving bottle (1), liquid biased sample is designated as G3, withdraws all devices,
Step 3: device gas recyclability is identified:
1) at one, carry out on without plant level land, cover bottomless organic glass case, with fine earth along base bedding compacting, air intake opening connects gas generating unit with sebific duct, gas outlet connects gas receiving bottle (1), opens all air inlets, the entrance of giving vent to anger, balance 15 minutes, 2) in the gas reaction bottle (4) in gas generating unit, add 99% abundance, the Na of 1mg
2 13cO
3solid sample 2, Na now
2 13cO
3solid sample 2 is designated as G4, with emulsion tube, bottomless one of them air intake opening of organic glass case is connected with gas generating unit, close other three air intake opening switches and all gas outlets switch, with syringe, to the gas reaction bottle (4) in gas generating unit, inject 0.1N hydrochloric acid solution 5ml, after a few minutes, by funnel external in gas generating unit, make gas reaction bottle (4) fill with water, air is all discharged, close the air intake opening that connects gas generating unit, withdraw gas generating unit, the water of the gas reaction bottle (4) in turned letter gas generating unit, the standing 2h of whole device, 3) in the gas receiving bottle (1) in four gas absorbing devices, respectively add 100g monoethanolamine, with emulsion tube, four gas outlets of bottomless organic glass case are connected with four gas absorbing devices, with sebific duct, four vacuum pumps (3) are connected with four gas absorbing devices, 4) start vacuum pump (3), open all gas outlets, carry out gas recovery, after 1 minute, open all air intake openings, after about 15 minutes, close all air intake openings, gas outlet, close vacuum pump (3), stop reclaiming, 5) every morning repeats above-mentioned 2) and 4) each 2 times, the time is respectively 6:00-8:00 and 8:30-10:30, repeats 2 afternoon) and 4) respectively twice, the time is respectively 13:00-15:00 and 15:30-17:30, in that afternoon, during 18:00-6:00 the next morning, open vacuum pump (3), and open all air intake openings of bottomless organic glass case, gas outlet, continue receiver gases, withdraw gas absorbing device morning after 6:00, now in all gas receiving bottle (1), liquid biased sample is designated as G5,
Step 4: local atmospheric carbon dioxide is collected:
At one, carry out on without plant level land, cover bottomless organic glass case, with fine earth along base bedding compacting, air intake opening connects gas generating unit with sebific duct, gas outlet connects gas receiving bottle (1), open all air inlets, the entrance of giving vent to anger, balance 15 minutes, in gas absorbing device, in gas receiving bottle (1), add 100ml monoethanolamine, with emulsion tube, connect gas outlet and gas absorbing device, with sebific duct, vacuum pump (3) is connected with gas absorbing device, open gas outlet switch, start vacuum pump (3), carry out gas recovery, after about 15 minutes, stop reclaiming, in gas receiving bottle (1), fluid sample is designated as G6,
Step 5: step 1 to step 4 every middle of a month is carried out once, experiment is carried out 1 year;
Step 6: carry out carbon source and carbon remittance and calculate:
Sample G1, G2, G3, G4, G5 and G6 measure respectively concentration of carbon, and prior art and standard for assay method are calculated total carbon, are designated as respectively C1, C2, C3, C4, C5 and C6, and unit is mg;
Sample G1, G2, G3, G4, G5 and G6 measure respectively on isotope ratio mass spectrometer
13c abundance, is designated as respectively α 1, α 2, α 3, α 4, α 5 and α 6;
Carbon source and carbon remittance computing method:
Device gas reclaiming rate calculates:
β(％)＝C5(α5-α6)/C4(α4-α6)×100
Spike in single test
13c amount:
χ1(mg)＝C1(α1-α6)，
Every m once
2vegetation first day reclaims spike
13c amount:
χ2(mg)＝C2(α2-α6)/β，
Experiment second day does not have
13c mark, collection
13c breathes to produce, one day every m
2vegetation breathes and produces spike
13c amount:
χ3(mg)＝C3(α3-α6)/β，
Suppose
12c and
13c photosynthetic fixing and breathe in there is no selectivity, every mg spike
13the total carbon of C representative is:
φ(mg)＝1000C0/χ1，
Wherein 1000 is the volume L of organic glass case, and C0 is atmosphere concentration of carbon mg/L,
Experiment second day does not have
13c mark, collection
13c breathes to produce, one day every m
2vegetation breathes carbon amount:
A＝φ×χ3，
Experiment first day photosynthesis of plant has been fixed
13c, plant respiration discharges again simultaneously
13c, spike in each test
13c amount χ 1 and each every m
2vegetation first day reclaims spike
13the difference of C amount χ 2, within one day, every m2 vegetation is only fixing
13c amount, one day every m
2the clean fixing carbon amount of vegetation:
B＝φ×(χ1-χ2)，
One day every m
2the fixing carbon amount of plant photosynthesis is one day every m
2the clean fixing one day every m of carbon amount of vegetation
2vegetation breathes carbon amount sum:
D＝φ×(χ1-χ2)+φ×χ3
Average every day every m
2vegetation breathes carbon amount (A
on average, mg), average every day every m
2the clean fixing carbon amount (B of vegetation
on average, mg) with average every day of every m
2carbon amount (the D that plant photosynthesis is fixing
on average, mg) be respectively:
A
on average=(∑ A)/12/5,
B
on average=(∑ B)/12/5,
D
on average=(∑ D)/12/5,
Annual 365 days, per hectare was 10000m
2, breathe carbon amount (A per hectare vegetation year
year, ton), per hectare vegetation year clean fixed carbon amount (B
year, ton) and per hectare vegetation year fixed carbon amount (D
year, ton) be respectively:
A
year=365 * 10
-9a
on average(ton/hectare),
B
year=365 * 10
-9b
on average(ton/hectare),
D
year=365 * 10
-9b
on average(ton/hectare).
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| CN106124723A (en) * | 2016-08-23 | 2016-11-16 | 中国地质科学院岩溶地质研究所 | A kind of method of real time on-line monitoring water plant karst carbon remittance effect |
| CN106124723B (en) * | 2016-08-23 | 2018-01-09 | 中国地质科学院岩溶地质研究所 | A kind of method of real time on-line monitoring water plant karst carbon remittance effect |
| CN108093930A (en) * | 2017-12-12 | 2018-06-01 | 西北师范大学 | A kind of photosynthetic plant tracer case |
| CN111044687A (en) * | 2020-01-09 | 2020-04-21 | 中国科学院东北地理与农业生态研究所 | Device for in-situ labeling C3 crop photosynthetic carbon in field and application |
| CN113487466A (en) * | 2021-06-29 | 2021-10-08 | 浙江数秦科技有限公司 | Carbon sink certificate system based on block chain |
| CN113487466B (en) * | 2021-06-29 | 2024-02-27 | 浙江数秦科技有限公司 | Carbon sink certificate system based on block chain |
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