CN106248444A - Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof - Google Patents
Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof Download PDFInfo
- Publication number
- CN106248444A CN106248444A CN201610735932.XA CN201610735932A CN106248444A CN 106248444 A CN106248444 A CN 106248444A CN 201610735932 A CN201610735932 A CN 201610735932A CN 106248444 A CN106248444 A CN 106248444A
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- gas
- soil respiration
- collection
- stable isotope
- carbon
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2226—Sampling from a closed space, e.g. food package, head space
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
Abstract
The invention discloses a kind of soil respiration CO2The collection of gas and carbon stable isotope value method of testing thereof, including: the collection of soil respiration carbon dioxide sample, by sample in intervals collection collection device；CO in sample2The test of concentration and the test of isotope value；Soil respiration CO is obtained according to derivation formula2Carbon stable isotope value.The soil respiration CO of the present invention2Sample collection device makes the simplest, and cost is lower, carries more convenient；Soil respiration CO2The analysis method of carbon stable isotope, analysis result is the most accurate.
Description
Technical field
The invention belongs to carbon stable isotope value technical field of measurement and test, particularly relate to a kind of soil respiration CO2Adopting of gas
Collection and carbon stable isotope value method of testing thereof.
Background technology
In recent years, Terrestrial Ecosystem Carbon Cycle process is to CO in air2The change of gas concentration plays vital
Effect.Global soil is the carbon storehouse that terrestrial ecosystems are maximum, comprises about 1500PgC (Schimel, 1995), soil simultaneously
Also being the potential Yuan Hehui of atmospheric carbon, the minor variations of carbon in soil content will make CO in air2Concentration changes a lot.
On the one hand in the decomposition mineralising of terrestrial ecosystems carbon and conversion process produced greenhouse gases by plant and soil
Enter air so that atmospheric greenhouse gas concentration increases；On the other hand, the increase of atmospheric greenhouse gas concentration is caused
Climate warming may cause Land-Use and the vegetation pattern etc. of terrestrial ecosystems to change, and then makes in soil
Organic Carbon Storage changes.Research is pointed out, the change of the soil organic matter of 10% is equivalent to what mankind's activity in 30 years caused
All of CO2Burst size.Terrestrial Ecosystem Carbon Cycle and global climate influence each other, and therefore, inquire into soil CO2Source converge effect
The mechanism should intercepted and captured with soil carbon and approach, saturated for alleviating terrestrial ecosystems carbon, delay and the speed of sluggish global warming
Rate and scale have important and urgent meaning.Soil Respiration refers to that the carbon in carbon base in soil is with CO2Form be discharged into
Process in air, soil respiration mainly includes that soil microorganism breathes (heterotrophic respiration) and root breath (autotrophic respiration) two
Point.Wherein root system respiration is the important component part of soil respiration, and its ratio shared in soil respiration determines that land is raw
State system Carbon balance relation and the key parameter of soil carbon density speed, for assessment whole world change to soil carbon storage and
May affecting of circulation is most important.Stable isotope dilution is based primarily upon root respiration and the microbial decomposition soil organism
During general without CO2Fractionation effect, and the soil organism and root system of plant have the same place value of different carbon, thus calculate and are unearthed
Earth breathes each component contributions.The method is the same with isotope-labelling method, it is to avoid become the method such as synthetic method, trench method to from
So impact of environment, data are closer to truth.The method success or failure it is crucial that truly obtain soil respiration CO2Carbon is stable
Isotope value.
Currently, with respect to greenhouse gases CO2Discharge (or absorption) method for measuring mainly has box method, microclimate method and chemistry
Method.The most box method is by being divided into static box method and dynamic box method；Microclimate method be divided into Chamber technique, energy budget method,
Aerodynamic method and mass balance approach.Except also include the methods such as super large case long light path infra-red chromatography, gradient method, but these
Method causes promoting the use of the most difficult because technology is not exclusively ripe or instrument and equipment cost is too high.Wherein static chamber due to
Itself possess certain advantage, be to measure soil respiration CO2Main Means, it typically uses lucite to make, area one
As be 50cm × 50cm × 15cm.Existing soil respiration CO2Gathering the static chamber used typically uses lucite to make, face
Long-pending generally 50cm × 50cm × 15cm.Domestic existing documents and materials show, soil respiration CO2The survey of carbon stable isotope value
Surely using alkali absorption method, alkali absorption method can not truly reflect soil respiration CO2Carbon stable isotope value, this be because of
For alkali absorption method except absorbing the CO that a part of soil discharges2, original CO in simultaneously also absorbing static chamber2Gas,
Therefore soil respiration CO is reduced2The accuracy of carbon stable isotope value.
Summary of the invention
It is an object of the invention to provide a kind of soil respiration CO2The collection of gas and carbon stable isotope value test side thereof
Method, it is intended to solve current method and reduce soil respiration CO2The problem of the accuracy of carbon stable isotope value.
The present invention is achieved in that a kind of soil respiration CO2The collection of gas and carbon stable isotope value test side thereof
Method, collection and the carbon stable isotope value method of testing thereof of described soil respiration CO2 gas comprise the following steps:
The collection of soil respiration carbon dioxide sample, by sample in intervals collection collection device；
CO in sample2The test of concentration and the test of isotope value；
Soil respiration CO is obtained according to derivation formula2Carbon stable isotope value.
Described method of testing farther includes soil respiration CO2The acquisition method of sample, particularly as follows:
Sample duration is 30min, respectively extraction cover case in 0,10,20 and 30min time gaseous sample；Sampling every time
Time extraction case in gas 500mL be placed in sealed air pocket, for CO2Gas concentration is analyzed and the mensuration of carbon stable isotope value.
Described method of testing farther includes soil respiration CO2The concentration of sample and carbon stable isotope values determination method,
Use LGR carbon dioxide laser isotope analysis instrument to measure concentration and carbon stable isotope value simultaneously.
Described method of testing farther includes soil respiration CO2The derivation method of carbon stable isotope value, particularly as follows:
According to CO in the static chamber that 0,10,20 and 30min each moment measured2Gas concentration and carbon stable isotope value,
Construct CO2Gaseous carbon stable isotope value and CO2Linear equation between the inverse of gas concentration, obtains soil respiration CO2Carbon
Stable isotope value；
Soil respiration CO2The reckoning foundation of carbon stable isotope value
C1=C0+C2, (1)
Wherein: C1, C0And C2The CO of t in expression static chamber respectively2Concentration, t0The CO in moment2Concentration and soil respiration
The CO discharged2Concentration；Every component of formula (1) is multiplied by respective CO respectively2Isotope ratio (δ13C), it becomes possible to
To stable isotope13The mass balance equation formula (2) of C；
δ13C1×C1=δ13C0×C0+δ13C2×C2, (2)
Formula (1) and (2) are combined and obtain formula (3):
δ13C1=C0×(δ13C0-δ13C2)×(1/C1)+δ13C2, (3)
Wherein δ13C2It is soil respiration CO2The carbon stable isotope value of gas, δ13C1VS(1/C1) straight line Y-axis cut
Away from being δ13C2。
Another object of the present invention is to provide a kind of described soil respiration CO2The collection of gas and carbon stable isotope thereof
The collection soil respiration CO of value method of testing2The easy device of gas, described collection soil respiration CO2The easy device bag of gas
Including gas collecting bottle (2) and sampler bag (6), described gas collecting bottle (2) is arranged in the middle of pedestal (1), and described syringe (7) passes through tee T
(8) with airway (3), sampler bag (6) is fixing is connected, described airway (3) other end is arranged on gas collecting bottle (2) bottom, described
Gas collecting bottle (2) bottleneck is provided with thermometer (5), is provided with sealing rubber plug (4), described tee T (8) in described gas collecting bottle (2) bottleneck
Being provided with manually-operated gate (9), described gas collecting bottle (2) bottom is provided with CO2 sensor (10), and described pedestal (1) one end is provided with display
Device (11), described CO2 sensor (10) is electrically connected with display (11).
Further, described gas collecting bottle (2) is 18.9 liters of pure water barrels.
Further, three port junctions of described tee T (8) are provided with sealing ring.
The soil respiration CO that the present invention provides2The collection of gas and carbon stable isotope value method of testing thereof, use barreled
Mineral water bottle processes through simple transformation, and the static chamber cost of manufacture comparing the making of existing lucite is lower, makes
More convenient, and weight is lighter, carries more convenient；Original gas CO in avoiding static chamber2To soil respiration CO2Carbon is stably same
The impact of position element value so that measurement result is the most accurate.The soil respiration CO of the present invention2Sample collection device makes the simplest
Single, cost is lower, carries more convenient；Soil respiration CO2The analysis method of carbon stable isotope, it is to avoid static interior original CO2
Gas disturbs, and analysis result is the most accurate.
The soil respiration CO recorded by alkali absorption method2The carbon stable isotope value of gas is all tried to achieve between this method
Soil respiration CO2Original CO in the carbon stable isotope value of gas and static chamber2Between gaseous carbon stable isotope value, show alkali
Liquid absorption process not only absorbs the CO of soil respiration release2Gas, and original CO in absorbing static chamber2Gas.
Accompanying drawing explanation
Fig. 1 is the soil respiration CO that the embodiment of the present invention provides2The collection of gas and carbon stable isotope value test side thereof
Method flow chart.
Fig. 2 is the collection soil respiration CO that the embodiment of the present invention provides2The apparatus structure schematic diagram of gas；
In figure: 1, pedestal；2, gas collecting bottle；3, airway；4, sealing rubber plug；5, thermometer；6, sampler bag；7, injection
Device；8, tee T；9, manually-operated gate；10、CO2Sensor；11, display.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, to the present invention
It is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Below in conjunction with the accompanying drawings the application principle of the present invention is explained in detail.
As it is shown in figure 1, the soil respiration CO of the embodiment of the present invention2The collection of gas and carbon stable isotope value test side thereof
Method comprises the following steps:
S101: soil respiration CO2The easy device of collection and confinement of gases makes: formed by the transformation of barreled mineral water bucket, remaining dress
Put accessory and also include rubber stopper, tee T, syringe, vacuum oil, base etc.；
S102: soil respiration CO2The analysis method of carbon stable isotope value；
S103: the collection of soil respiration carbon dioxide sample, by sample in intervals collection collection device；
S104: CO in sample2The test of concentration and the test of isotope value；
S105: obtain soil respiration CO according to derivation formula2Carbon stable isotope.
The soil respiration CO of the embodiment of the present invention2Collection and the carbon stable isotope value method of testing thereof of gas specifically include
Following steps:
The simplified collection device of 1, soil respiration carbon dioxide sample makes
1), choose airtight intact barreled mineral water bucket, excise mineral water bottle base section, and according to after bottom excision
Barreled mineral water bottom of the barrel diameter makes suitable base；2), with card punch, rubber stopper is punched, insert glass
Pipe；3), smear vacuum oil on rubber stopper, fill at the thin mouth of barreled mineral water bucket；4), rubber is connected with one end of rubber hose
Glass tubing beyond the Great Wall；One interface of other end connecting tee pipe；5), the another two interface of tee T, one access rubber soft
Pipe, bleeds with standby syringe；Another interface gives over to connect gas sampling bag.
2, soil respiration CO2The collection of sample
Sample duration is 30min, respectively extraction cover case in 0,10,20 and 30min time gaseous sample.Sampling every time
Time extraction case in gas during about 500mL is placed in sealed air pocket, for CO2Gas concentration is analyzed and the survey of carbon stable isotope value
Fixed.
3, soil respiration CO2The concentration of sample and carbon stable isotope pH-value determination pH
Use LGR carbon dioxide laser isotope analysis instrument to measure concentration and carbon stable isotope value simultaneously.
4, soil respiration CO2The derivation of carbon stable isotope value
According to CO in the static chamber that 0,10,20 and 30min each moment measured2Gas concentration and carbon stable isotope value,
Construct CO2Gaseous carbon stable isotope value and CO2Linear equation between the inverse of gas concentration, obtains soil respiration CO2Carbon
Stable isotope value.
5, soil respiration CO2The reckoning foundation of carbon stable isotope value:
C1=C0+C2, (1)
Wherein: C1, C0And C2The CO of t in expression static chamber respectively2Concentration, t0The CO in moment2Concentration and soil respiration
The CO discharged2Concentration.Every component of formula (1) is multiplied by respective CO respectively2Isotope ratio (δ13C), it becomes possible to
To stable isotope13The mass balance equation formula (2) of C.
δ13C1×C1=δ13C0×C0+δ13C2×C2, (2)
Formula (1) and (2) are combined and obtain formula (3):
δ13C1=C0×(δ13C0-δ13C2)×(1/C1)+δ13C2, (3)
Wherein δ13C2It is soil respiration CO2The carbon stable isotope value of gas.As can be seen here, δ13C1VS(1/C1) straight line
Intercept in Y-axis is δ13C2。
A kind of collection soil respiration CO as shown in Figure 22The easy device of gas, including gas collecting bottle 2 and sampler bag 6, institute
Stating gas collecting bottle 2 and be arranged in the middle of pedestal 1, described syringe 7 is fixed by tee T 8 be connected with airway 3, sampler bag 6, described
Airway 3 other end is arranged on gas collecting bottle 2 bottom, and described gas collecting bottle 2 bottleneck is provided with thermometer 5, sets in described gas collecting bottle 2 bottleneck
Having sealing rubber plug 4, described tee T 8 to be provided with manually-operated gate 9, described gas collecting bottle 2 bottom is provided with CO2Sensor 10, described
Pedestal 1 one end is provided with display 11, described CO2Sensor 10 is electrically connected with display 11.
Further, described gas collecting bottle 2 is 18.9 liters of pure water barrels.
Further, three port junctions of described tee T 8 are provided with sealing ring.
This collection soil respiration CO2The easy device of gas, uses barreled mineral water bucket to process through simple transformation,
The static chamber cost of manufacture comparing the making of existing lucite is lower, makes more convenient, and weight is lighter, carries more square
Just, there is more practical use value.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any amendment, equivalent and the improvement etc. made within god and principle, should be included within the scope of the present invention.
Claims (7)
1. a soil respiration CO2The collection of gas and carbon stable isotope value method of testing thereof, it is characterised in that described soil
Breathe CO2Collection and the carbon stable isotope value method of testing thereof of gas comprise the following steps:
The collection of soil respiration carbon dioxide sample, by sample in intervals collection collection device；
CO in sample2The test of concentration and the test of isotope value；
Soil respiration CO is obtained according to derivation formula2Carbon stable isotope value.
2. soil respiration CO as claimed in claim 12The collection of gas and carbon stable isotope value method of testing, its feature
Being, described method of testing farther includes soil respiration CO2The acquisition method of sample, particularly as follows:
Sample duration is 30min, respectively extraction cover case in 0,10,20 and 30min time gaseous sample；Take out during sampling every time
In taking case, gas 500mL is placed in sealed air pocket, for CO2Gas concentration is analyzed and the mensuration of carbon stable isotope value.
3. the collection of soil respiration CO2 gas as claimed in claim 1 and carbon stable isotope value method of testing, its feature
Being, described method of testing farther includes soil respiration CO2The concentration of sample and carbon stable isotope values determination method, use
LGR carbon dioxide laser isotope analysis instrument measures concentration and carbon stable isotope value simultaneously.
4. soil respiration CO as claimed in claim 12The collection of gas and carbon stable isotope value method of testing, its feature
Being, described method of testing farther includes soil respiration CO2The derivation method of carbon stable isotope value, particularly as follows:
According to CO in the static chamber that 0,10,20 and 30min each moment measured2Gas concentration and carbon stable isotope value, construct
CO2Gaseous carbon stable isotope value and CO2Linear equation between the inverse of gas concentration, obtains soil respiration CO2Carbon is stably same
Position element value；
Soil respiration CO2The reckoning foundation of carbon stable isotope value
C1=C0+C2, (1)
Wherein: C1, C0And C2The CO of t in expression static chamber respectively2Concentration, t0The CO in moment2Concentration and soil respiration release
The CO gone out2Concentration；Every component of formula (1) is multiplied by respective CO respectively2Isotope ratio (δ13C), it becomes possible to obtain steady
Qualitative isotope13The mass balance equation formula (2) of C；
δ13C1×C1=δ13C0×C0+δ13C2×C2, (2)
Formula (1) and (2) are combined and obtain formula (3):
δ13C1=C0×(δ13C0-δ13C2)×(1/C1)+δ13C2, (3)
Wherein δ13C2It is soil respiration CO2The carbon stable isotope value of gas, δ13C1VS(1/C1) straight line Y-axis intercept i.e.
For δ13C2。
5. a soil respiration CO as claimed in claim 12The collection of gas and the collection of carbon stable isotope value method of testing thereof
Soil respiration CO2The easy device of gas, it is characterised in that described collection soil respiration CO2The easy device of gas includes gas collection
Bottle (2) and sampler bag (6), described gas collecting bottle (2) is arranged in the middle of pedestal (1), described syringe (7) by tee T (8) and
Airway (3), sampler bag (6) are fixing to be connected, and described airway (3) other end is arranged on gas collecting bottle (2) bottom, described gas collecting bottle
(2) bottleneck is provided with thermometer (5), is provided with sealing rubber plug (4) in described gas collecting bottle (2) bottleneck, and described tee T (8) is provided with
Manually-operated gate (9), described gas collecting bottle (2) bottom is provided with CO2 sensor (10), and described pedestal (1) one end is provided with display (11),
Described CO2 sensor (10) is electrically connected with display (11).
6. collection soil respiration CO as claimed in claim 52The easy device of gas, it is characterised in that described gas collecting bottle (2)
It is 18.9 liters of pure water barrels.
7. collection soil respiration CO as claimed in claim 52The easy device of gas, it is characterised in that described tee T (8)
Three port junctions be provided with sealing ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610735932.XA CN106248444A (en) | 2016-08-26 | 2016-08-26 | Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof |
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| CN201610735932.XA CN106248444A (en) | 2016-08-26 | 2016-08-26 | Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof |
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| CN201610735932.XA Withdrawn CN106248444A (en) | 2016-08-26 | 2016-08-26 | Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106680044A (en) * | 2016-12-29 | 2017-05-17 | 上海交通大学 | Methane collection device and methane collection method for rice stable isotope labeling |
| CN112924249A (en) * | 2021-01-25 | 2021-06-08 | 中国科学院地质与地球物理研究所 | Sampling and injecting device and method for carbon isotope analysis of slurry gas |
| CN113945681A (en) * | 2020-07-17 | 2022-01-18 | 中国科学院沈阳应用生态研究所 | Survey forest ecosystem component respiratory delta13C device and method |
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| CN106680044A (en) * | 2016-12-29 | 2017-05-17 | 上海交通大学 | Methane collection device and methane collection method for rice stable isotope labeling |
| CN113945681A (en) * | 2020-07-17 | 2022-01-18 | 中国科学院沈阳应用生态研究所 | Survey forest ecosystem component respiratory delta13C device and method |
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Application publication date: 20161221 |