CN106680044A - Methane collection device and methane collection method for rice stable isotope labeling - Google Patents
Methane collection device and methane collection method for rice stable isotope labeling Download PDFInfo
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- CN106680044A CN106680044A CN201611246673.0A CN201611246673A CN106680044A CN 106680044 A CN106680044 A CN 106680044A CN 201611246673 A CN201611246673 A CN 201611246673A CN 106680044 A CN106680044 A CN 106680044A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
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Abstract
The invention provides a methane collection device and a methane collection method for rice stable isotope labeling. The device comprises a gas-insulating and light-transmitting cover body, a chassis, a bracket, a CO2 consumption part, an isotope-labeled CO2 production part, a gas mixing part, a gas collection pipeline and a CO2 detector connecting part, wherein the chassis is in seal connection with the cover body in a detachable way; the bracket is arranged in the cover body and is used for supporting the cover body; the CO2 consumption part comprises an alkali tray and an alkali adding pipeline; the alkali adding pipeline is connected with the cover body in a sealing way and is vertical to the cover body, the alkali tray is arranged under the alkali adding pipeline, and the upper end of the alkali adding pipeline is provided with an alkali adding mouth extending out of the cover body; the isotope-labeled CO2 production part comprises a reaction tray and an acid adding pipeline; the acid adding pipeline is connected with the cover body in a sealing way and is vertical to the cover body, the reaction tray is arranged under the acid adding pipeline, and the upper end of the acid adding pipeline is provided with an acid adding mouth extending out of the cover body. The raw materials used by the device and the method are simple and easy to obtain, so that the cost is lowered; furthermore, the device is high in airtight property and easy to operate.
Description
Technical field
The present invention relates to the research field such as stable isotopic tracer, greenhouse gases collection, more particularly to a kind of to be used for water
The methane harvester of rice cold labeling and method.
Background technology
Point out according to the 5th assessment report of IPCC, global seismic mean temperature increases 0.85 DEG C between 1880-2012, and
The earth will persistently warm, and predicting to 2025 and 21 century end may cause Global Temperature to increase by 1 DEG C and 3 DEG C respectively.People
It is believed that global warming be mainly due to greenhouse gases anthropogenic discharge amount increase, wherein, come from the greenhouse of agricultural sources
Gas discharge accounts for the 20%-35% of Global Emissions of Greenhouse Gas total amount, is only second to the energy, occupies second.Correlational study shows, agriculture
Field soil is most important greenhouse gas emission source on the earth, and highly important status is occupied in Global Emissions of Greenhouse Gas.
CO2、CH4And N2O is most important three kinds of greenhouse gases.According to statistics, CH is discharged in annual rice field4Total amount account for total release
12%.Oryza sativa L. is the staple food grain of global 50% population, even more important in Asia.China is second-biggest-in-the-world Rice Cropping country, is planted
Area accounts for the whole world 20% is planted, total output accounts for the whole world 30%.Thus it is guaranteed that while rice terrace increases production, reducing paddy methane
Discharge is significant.Soil carbon circulating research has become one of agrology study hotspot, carbon isotope tracer over nearly 20 years
Technology has been widely used in the research in terms of soil carbon conversion and circulation, and plants carbon isotope labelling method is that research plant is residual
Body decompose in soil and convert and plant to soil be input into organic carbon basis, be also used for study plant photosynthesis
Characteristic and plant absorption CO2The organic carbon of synthesis is in its internal operating and distribution.But due to carbon isotope labelling raw material
It is expensive, CO2Easily diffusion again, particularly14C-CO2Category radgass, therefore deposit when plants carbon isotope labelling is carried out
In certain technical difficulty.The isotope-labeled device of current Study On Rice is heavier, and involves great expense.
The content of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of be used for Oryza sativa L. stable isotope
The methane harvester of labelling and method, the raw material of the device simply easily takes, and reduces cost, and seal is strong, easily
Operation.
For achieving the above object and other related purposes, the present invention provides a kind of first for Oryza sativa L. cold labeling
Alkane harvester, including：The cover body of trapping printing opacity；Chassis, is connected with the cover body detachable-type sealed；Support, is arranged at institute
State in cover body, for supporting the cover body；CO2Consumable part, including alkali pallet and alkali adds pipeline, the alkali add pipeline with
Cover body is tightly connected and is vertically arranged, and the alkali adds pipeline lower section to be provided with the alkali pallet, and the alkali adds pipeline upper end
It is provided with the alkali plus entrance for stretching out the cover body；Isotope marks CO2Generating means, including reaction pallet and acid addition pipeline,
The acid adds pipeline to be tightly connected and be vertically arranged with the cover body, and the acid adds pipeline lower section to be provided with the reaction support
Disk, the acid adds the upper end of pipeline to be provided with the acid plus entrance for stretching out the cover body；Mixed gas part, is arranged at the cover body
It is interior；Gas collecting pipeline, it connects with the cover body internal gas and is tightly connected with the cover body, and its end is provided with stretches out
The gas collecting outlet of the cover body；CO2Detector connection member, is tightly connected with the cover body, its one end be arranged at institute
State the CO outside cover body2Detector is connected.
Preferably, the support include upper horizontal tube group and with upper horizontal tube group VERTICAL TUBE group connected vertically, institute
State horizontal tube group and the VERTICAL TUBE group to be constituted by pvc pipe, the upper horizontal tube group is provided with multiple four-way unions, institute
State CO2Consumable part, isotope marks CO2Generating means, the mixed gas part and the gas collecting pipeline pass through respectively
Four-way connection is connected with the upper horizontal tube group.
It is highly preferred that the upper horizontal tube group is square, it includes horizontal tube on the first of annular connection successively, on second
Horizontal tube, horizontal tube on horizontal tube and the 4th on the 3rd, on described second on horizontal tube and the described 4th horizontal tube two ends point
Four-way connection is not provided with it.
Preferably, the alkali adds entrance to be provided with rubber sheet gasket with the cover body junction.
It is highly preferred that the lower end of four-way connection described in one and the alkali add pipeline to be connected, upper end with nylon spacer
The first locking nut be connected, the first locking nut upper end is connected with alkali seal closure, first locking nut with it is described
The rubber sheet gasket is installed between alkali seal closure.
Preferably, it is glass tubule that the acid adds pipeline, and the glass tubule is outer to be provided with the cover body junction
First rubber stopper.
It is highly preferred that the upper end of four-way connection described in be connected with the second locking nut of nylon spacer, described the
One locking nut upper end is connected with first rubber stopper.
Preferably, the mixed gas part is fan, and the fan Jing electric wire external power supplys, the cover body is provided with for described
The electric line outlets that electric wire is passed, the electric line outlets are tightly connected with the cover body.
Preferably, the quantity of the fan is that, no less than two, the fan may be contained within the support and its wind direction
Towards the centre position of the cover body.
It is highly preferred that the upper end of four-way connection described in is connected with the 3rd locking nut with nylon spacer, the electricity
Line is accessed in upper horizontal tube, is then passed from the 3rd locking nut from the both positive and negative polarity electric wire of fan.
Preferably, latex is scribbled at the electric line outlets.
Preferably, the gas collecting pipeline is glass tubing, and described glass tubing one end is provided with the cover body junction
Second rubber stopper, the glass tubing other end is cased with the latex tubing with three-way valve.
It is highly preferred that the upper end of four-way connection described in be connected with the 4th locking nut of nylon spacer, described the
The upper end of four locking nuts is connected with second rubber stopper.
Preferably, the CO2Detector connecting pipe is provided with the with nylon spacer the 5th with the cover body junction
Locking nut.
It is highly preferred that the CO2Detector attachment means include CO2Detector connecting pipe 33, CO2Detector connecting tube
Road support, the 5th locking nut and CO2Detection seal closure, the CO2Detector connecting pipe passes through the CO2Detector connects
Pipeline support is connected with the upper horizontal tube group, the CO2One end of detector connecting pipe is connected with the side of the cover body,
The CO2The 5th locking nut being provided with nylon spacer of detector connecting pipe and the cover body junction, it is described
The outside of the 5th locking nut and the CO2Detection seal closure is connected.
Preferably, the methane harvester also includes thermometer, and the thermometer is arranged in the cover body, for examining
The temperature surveyed in cover body.
Invention additionally discloses a kind of methane acquisition method of Oryza sativa L. cold labeling, is adopted using methane described above
Acquisition means are carried out, and are comprised the following steps：
1) it is placed in the rice seedling turned green is potted plant in the methane harvester, will be equipped with13Carbonate after C flag is molten
The container of liquid is placed on the reaction pallet, to the cover body and the direct water filling in the base junction, forms complete sealing
Casing；
2) using the CO2CO in detector detection case body2Concentration, if CO2Excessive concentration, by CO2Consumable part is thrown
Enter alkali, absorb CO2；
3) Jing isotope marks CO2Generating means to13Carbonate solution acid adding after C flag carries out isotopic tracing mark
Note, according to test needs, calculates the volume for instilling acid, is marked by sour chemical reaction；
4) taken at regular intervals gas sample, is connected the fan with power supply during collection gas sample by the electric wire, protects
Demonstrate,prove the casing inner air flow uniform.
As described above, the methane harvester and method for Oryza sativa L. cold labeling of the present invention, with following
Beneficial effect：
The present invention is placed in plant in closed labelling case, observable by whole gas labelling and harvester13The same positions of C
Element is produced in the environment of rice soil anaerobism by the interaction between plant-soil-microorganism13CH4Discharge capacity, be
Paddy methane emission mechanism research offer science is supported.Cultivation also can be taken off plant after terminating, and obtain isotope-labeled plant
And microorganism, can preferably explain transfer and circulation of the carbon in whole paddy system.The inventive method is easy to operate, sealing
Property it is high, material simply obtains, is suitable for carrying out carbon isotope labelling to various plants.
The present invention is light using raw material material, easily assembled, is easy to carry or moves；Can easily add in confined conditions
Acid and CO of the alkali in reactive tank, in balance box2, the CO needed on the one hand supply plant photosynthesis grow2, on the other hand
Can at any time monitoring system implants-soil-bacteria metabolism produce CH4Discharge capacity；In addition, the labelling of stable isotope
Long-term continued labelling, or discontinuous pulses labelling can be designed as according to test needs；Casing is easy to open in the present invention, can be plant
Strain addition fertilizer and moisture.
Description of the drawings
Fig. 1 is shown as the vertical of methane harvester for Oryza sativa L. cold labeling of the present invention in an embodiment
Body structural representation.
Component label instructions：
1st, on cover body 211, first on horizontal tube 212, second on horizontal tube the 213, the 3rd on horizontal tube the 214, the 4th
Horizontal tube 22, middle horizontal tube 23, lower horizontal tube 24, vertical tube 25,26,90 ° of four-way connection on horizontal tube the 215, the 4th
Joint 27, three way cock 31, CO2Detection seal closure 32, the 5th locking nut 33, CO2Detector connecting pipe 34, CO2
Detector connecting pipe support 35, direct joint 41, alkali seal closure 42, the first locking nut 43, alkali add pipeline 44,
Alkali pallet 45, alkali tray support 51, glass tubule 52, the first rubber stopper 53, the second locking nut 54, reaction pallet
Support 8, chassis at 55, reaction pallet 61, the second rubber stopper 62, glass tubing 71, fan 72, electric wire 73, electric line outlets
9th, bucket.
Specific embodiment
Hereinafter embodiments of the present invention are illustrated by particular specific embodiment, those skilled in the art can be by this explanation
Content disclosed by book understands easily other advantages and effect of the present invention.
Fig. 1 is referred to it should be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc., only to coordinate
Content disclosed in bright book, so that those skilled in the art understands and reads, is not limited to enforceable limit of the invention
Fixed condition, therefore do not have technical essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size, not
Under affecting effect that can be generated of the invention and the purpose to be reached, all should still fall in disclosed technology contents institute
In the range of covering.Meanwhile, in this specification it is cited as " on ", D score, "left", "right", " centre " and " one " etc.
Term, is merely convenient to understanding for narration, and is not used to limit enforceable scope of the invention, the change of its relativeness or tune
It is whole, under without essence change technology contents, when being also considered as enforceable category of the invention.
Embodiment provides a kind of methane harvester for Oryza sativa L. cold labeling, can be widely used in ecology
, botany, Crop Science, microbiology, chemistry, environmental project etc. need to gather the field such as gas or isotopic tracing.Please
Refering to Fig. 1, Fig. 1 is shown as in embodiment the stereochemical structure of the methane harvester for being used for Oryza sativa L. cold labeling and illustrates
Figure, as illustrated, it includes cover body 1, support, chassis 8, CO2Consumable part, isotope marks CO2Generating means, mixed gas part,
Gas collecting pipeline and CO2Detector attachment means.
Cover body 1 is the cover body of trapping printing opacity.In embodiment, cover body is made up of hard trapping printing opacity PVC film.
Chassis 8 is connected with the cover body detachable-type sealed；In the present embodiment, cover body 1 is filled with the junction of chassis 8
Water, forms complete seal case.
Support is arranged in cover body 1, for supporting cover body.In the present embodiment, whole support is in cube, and support is arranged
On the reeded chassis 8 of band.
In the present embodiment, support includes that upper horizontal tube group, middle horizontal tube 22, lower horizontal tube 23, VERTICAL TUBE 24, three are connected
27,90 ° of joints 26 and four-way connection 25；Upper horizontal tube group, middle horizontal tube 22, lower horizontal tube 23 are vertical with VERTICAL TUBE 24 respectively
It is connected.Upper horizontal tube group includes in annular connection successively first on horizontal tube 211, second level on horizontal tube the 212, the 3rd
Horizontal tube 214 on pipe 213 and the 4th, and two ends be connected with horizontal tube 214 in level on second the 212, the 4th respectively the 5th
Upper horizontal tube 215, the two ends of horizontal tube 213 are provided with three way cock 27 on horizontal tube 211 and the 3rd on first, water on second
The two ends of horizontal tube 214 are provided with four-way connection 25 on flat pipe 212 and the 4th, horizontal tube on horizontal tube 211, second on first
212nd, it is connected by three way cock 27, four-way connection 25 respectively between horizontal tube 214 on horizontal tube 213 and the 4th on the 3rd.
One end of vertical tube 24 is provided with three way cock, and the other end is provided with four-way connection；Horizontal tube 211 and on first
The two ends of horizontal tube 213 pass sequentially through respectively one end of 27,90 ° of joints 26, three way cock of three way cock and vertical tube 24 on three
It is connected；Lower horizontal tube 23 is connected by four-way connection with the other end of vertical tube 24.
In the present embodiment, CO2Consumable part include the first seal closure 41, the first locking nut 42, alkali add pipeline 43,
Alkali pallet 44 and alkali tray support 45, alkali adds pipeline 43 by the vertical connection of horizontal tube 212 on four-way connection and second, and sets
The downside of horizontal tube 212 on second is placed in, alkali adds the lower section of pipeline 43 to be provided with alkali pallet 44, and the bottom of alkali pallet 44 is provided with alkali
Tray support 45, alkali tray support 45 is vertically connected by three way cock 46 with VERTICAL TUBE 24, plays the pallet effect of support alkali.With
Alkali adds the upper end of the connected four-way connection of pipeline 43 to be provided with the first locking nut 42 with nylon spacer, the first locking screw
Female 42 upper end is connected with alkali seal closure 41, and between the first locking nut 42 and alkali seal closure 41 rubber sheet gasket is provided with.Alkali adds
Entrance and the junction of cover body 1, it is inside and outside to be clamped by rubber sheet gasket, play sealing function.
In the present embodiment, it is pvc pipe that alkali adds passage, prevents caustic corrosion.
In the present embodiment, alkali is preferably sodium hydroxide or potassium hydroxide.
Isotope marks CO2Generating means, including reaction pallet 55 and acid addition pipeline, acid adds water on pipeline and the 4th
Flat pipe vertical connection, acid adds pipeline lower section to be provided with reaction pallet 55, and reaction pallet 55 bottom is provided with reaction pallet
Support 54, reaction tray support 54 is connected by three way cock with VERTICAL TUBE 24.The upper end of acid addition pipeline is provided with stretches out cover
The acid of body 1 adds entrance.In the present embodiment, it is glass tubule 51 that acid adds pipeline, prevents acid corrosion, horizontal tube 214 on the 4th
The upper end of four-way connection of one end the second locking nut 53 with nylon spacer is installed, the upper end of the second locking nut 53 peace
Equipped with the first rubber stopper 52, the glass tubule 51 stretched in cover body 1 is installed in the first rubber stopper 52, the first rubber stopper 52 both rose
To the effect of fixed glass tubule 51, cover body 1 is simultaneously clamped on, play sealing function.
In the present embodiment, acid is preferably hydrochloric acid.
In the present embodiment, react to place on pallet 55 in small beaker container, container and place carbonate solution, it is preferable that
Carbonate is sodium carbonate or potassium carbonate.
Mixed gas part is arranged in cover body.In the present embodiment, mixing gas part includes two fans 71, and fan 71 is symmetrically outstanding
Casing top is hung over, 45 ° are inclined, with rope pvc pipe crossbeam is fixed on.The one end of horizontal tube 212 is away from CO on second2Consumable part
One end be provided with four-way connection, the four-way connection upper end is provided with the 3rd locking nut with nylon spacer, the electricity of fan
Line 72 is accessed in upper horizontal tube, is then passed from the 3rd locking nut from the both positive and negative polarity electric wire of two fans.Electric wire goes out
73 are fixed with latex and are sealed at mouthful.After electric wire is picked out, connect accumulator, according to test demand, switch on power, it is ensured that gas in case
Body is uniform.
Gas collecting pipeline is connected with cover body internal gas and is tightly connected with the cover body, and its end is provided with stretches out cover
The gas collecting outlet of body.In the present embodiment, on the 4th horizontal tube 214 away from isotope marks CO2The one of generating means
End is provided with four-way connection, and the four-way connection upper end is provided with the 4th locking nut with nylon spacer, the 4th locking nut
Upper end is provided with gas collecting pipeline.Gas collecting pipeline is by the second rubber stopper 61 and the glass being installed in the second rubber stopper 61
Pipe 62 is constituted.
In the present embodiment, the second rubber stopper 61 is punched using card punch, and glass tubing 62 is inserted, the overcoat of glass tubing 62 breast
Sebific duct, each seam is with sealed membrane around guarantee sealing.
In the present embodiment, in latex tubing one easy three-way valve of another termination, injection can be first inserted during sampling
Device, three-way valve is screwed into into open state from closure state carries out gas collecting, gas bleeding outward；Collection terminates, and extracts sampling
Before syringe, three-way valve is screwed into closure state, extracts sampler, prevents gas from escaping.
CO2Detector connection member, is tightly connected with the cover body, its one end and the CO being arranged at outside the cover body2Detection
Device is connected.In the present embodiment, CO2Detector attachment means include the 3rd seal closure 31, the 3rd locking nut 32, CO2Detector
Connecting pipe 33 and CO2Detector connecting pipe supports 34, CO2The outside of detector connecting pipe 33 is provided with CO2Detector connects
Pipeline support 34, CO2Detector connecting pipe supports 34 to be connected with horizontal tube 215 on the 5th by direct joint 35, CO2Detection
The detection mouth of device connecting pipe 33 is located at the side of cover body 1, CO2Detector connecting pipe 33 is provided with the junction of cover body 1
The 5th locking nut 32 with nylon spacer, plays a part of to clamp cover body, and the outside of the 5th locking nut 32 is provided with CO2
Detection seal closure 31, it is ensured that sealing.
In the present embodiment, upper horizontal tube, middle horizontal tube 22, lower horizontal tube 23, VERTICAL TUBE 24, CO2Detector connecting tube
Road 34 and 27, the 90 ° of joints 26 of multiple three way cock being related to, four-way connection 25 and direct joint 35 are by PVC material structures
Into.
In the present embodiment, the specification of support is 60cm X 60cm X 100cm, and the specification of plastic chassis is 100cm X
100cmⅹ30cm；Bucket, volume is 50 liters, and bore 40cm is made up of hard PP plastics.Whole device can according to the size of plant,
The size of casing is adjusted, due to pvc pipe light quality, it is easy to carry or shift.
Embodiment also provide it is a kind of methane acquisition method is carried out using above-mentioned device, comprise the following steps：
1) rice seedling is transplanted in the bucket 9 equipped with soil, after 1-2 is all, seedling turns green, after adapted soil new environment,
Prepare next step test；
2) place a stent into on reeded chassis 8, bucket 9 is placed in support, while will be equipped with13After C flag
NaCO3The container of solution is placed on reaction pallet 55, and then cover body 1 is covered on support, to cover body 1 and the junction of chassis 8
Directly water filling, forms complete seal case；Using CO2CO in detector detection case body2Concentration, if CO2Excessive concentration, leads to
Cross CO2Consumable part puts into alkali, absorbs CO2；
3) carry out isotopic tracing labelling, according to test needs, calculate instill HCl volume, by HCl reaction start into
Line flag；
4) taken at regular intervals gas sample, is connected fan with power supply during collection gas sample, it is ensured that in casing by electric wire
Air circulation is uniform.
By whole gas labelling and harvester, Oryza sativa L. bucket is placed in closed labelling case, observable13C isotopes lead to
The interaction crossed between plant-soil-microorganism, it is produced in the environment of rice soil anaerobism13CH4Discharge capacity, is rice field
Discharge of methane study mechanism provides science and supports.Cultivation also can be taken off plant after terminating, and obtain isotope-labeled plant and micro-
Biology, can preferably explain transfer and circulation of the carbon in whole paddy system.The inventive method is easy to operate, sealing
Height, material is simply obtained, and is suitable for carrying out carbon isotope labelling to various plants.
The present invention is light using raw material material, easily assembled, is easy to carry or moves；Can easily add in confined conditions
HCL and CO of the NaOH solution in reactive tank, in balance box2, the CO needed on the one hand supply plant photosynthesis grow2, separately
On the one hand can CH that at any time monitoring system implants-soil-bacteria metabolism is produced4Discharge capacity；In addition, stable isotope
Labelling can according to test needs, be designed as long-term continued labelling, or discontinuous pulses labelling.So, the present invention effectively overcomes
Shortcoming of the prior art and have high industrial utilization.
The principle and its effect of above-described embodiment only illustrative present invention, it is of the invention not for limiting.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and the scope without prejudice to the present invention to above-described embodiment.Cause
This, such as those of ordinary skill in the art is complete with institute under technological thought without departing from disclosed spirit
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of methane harvester for Oryza sativa L. cold labeling, it is characterised in that include：
The cover body of trapping printing opacity；
Chassis, is connected with the cover body detachable-type sealed；
Support, is arranged in the cover body, for supporting the cover body；
CO2Consumable part, including alkali pallet and alkali addition pipeline, the alkali adds pipeline to be tightly connected with cover body and be vertically arranged,
The alkali adds pipeline lower section to be provided with the alkali pallet, and the alkali adds pipeline upper end to be provided with to stretch out the alkali of the cover body and add
Entrance；
Isotope marks CO2Generating means, including reaction pallet and acid addition pipeline, the acid adds pipeline close with the cover body
Envelope connects and is vertically arranged, and the acid adds pipeline lower section to be provided with the reaction pallet, and the upper end of the acid addition pipeline sets
It is equipped with the acid plus entrance for stretching out the cover body；
Mixed gas part, is arranged in the cover body；
Gas collecting pipeline, it connects with the cover body internal gas and is tightly connected with the cover body, and its end is provided with stretches
Go out the gas collecting outlet of the cover body；
CO2Detector connection member, is tightly connected with the cover body, its one end and the CO being arranged at outside the cover body2Detector phase
Even.
2. the methane harvester for Oryza sativa L. cold labeling according to claim 1, it is characterised in that：It is described
Support include upper horizontal tube group and with upper horizontal tube group VERTICAL TUBE group connected vertically, the upper horizontal tube group and described perpendicular
Straight tube group is constituted by pvc pipe, and the upper horizontal tube group is provided with four-way union, the CO2Consumable part, the isotope
Labelling CO2Generating means, the mixed gas part and the gas collecting pipeline are respectively by four-way connection and the upper horizontal tube
Group is connected.
3. the methane harvester for Oryza sativa L. cold labeling according to claim 1, it is characterised in that：It is described
Alkali adds entrance to be provided with rubber sheet gasket with the cover body junction；
And/or, it is glass tubule that the acid adds pipeline, and the glass tubule is outer to be provided with the first rubber with the cover body junction
Plug.
4. the methane harvester for Oryza sativa L. cold labeling according to claim 1, it is characterised in that：It is described
CO2Detector connecting pipe is provided with the locking nut with nylon spacer with the cover body junction.
5. the methane harvester for Oryza sativa L. cold labeling according to claim 1, it is characterised in that：It is described
Mixed gas part is fan, and the fan Jing electric wire external power supplys, the cover body is provided with the electric line outlets passed for the electric wire,
The electric line outlets are tightly connected with the cover body.
6. the methane harvester for Oryza sativa L. cold labeling according to claim 5, it is characterised in that：It is described
The quantity of fan be no less than two, the fan may be contained within the support and its wind direction towards the cover body interposition
Put.
7. the methane harvester for Oryza sativa L. cold labeling according to claim 5, it is characterised in that：It is described
Latex is scribbled at electric line outlets.
8. the methane harvester for Oryza sativa L. cold labeling according to claim 1, it is characterised in that：It is described
Gas collecting pipeline is glass tubing, and described glass tubing one end is provided with the second rubber stopper, the glass with the cover body junction
The pipe other end is cased with the latex tubing with three-way valve.
9., according to the arbitrary described methane harvester for Oryza sativa L. cold labeling of claim 1 to 8, its feature exists
In：Also include thermometer, the thermometer is arranged in the cover body.
10. a kind of methane acquisition method of Oryza sativa L. cold labeling, is adopted using the arbitrary described methane of claim 1 to 9
Acquisition means are carried out, and are comprised the following steps：
1) it is placed in the rice seedling turned green is potted plant in the methane harvester, will be equipped with13Carbonate solution after C flag
Container is placed on the reaction pallet, to the cover body and the direct water filling in the base junction, forms the case of complete sealing
Body；
2) using the CO2CO in detector detection case body2Concentration, if CO2Excessive concentration, by CO2Consumable part puts into alkali,
Absorb CO2；
3) Jing isotope marks CO2Generating means to13Carbonate solution acid adding after C flag carries out isotopic tracing labelling, root
Need according to test, calculate the volume for instilling acid, be marked by sour chemical reaction；
4) taken at regular intervals gas sample, is connected the fan with power supply during collection gas sample, it is ensured that institute by the electric wire
State casing inner air flow uniform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611246673.0A CN106680044A (en) | 2016-12-29 | 2016-12-29 | Methane collection device and methane collection method for rice stable isotope labeling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611246673.0A CN106680044A (en) | 2016-12-29 | 2016-12-29 | Methane collection device and methane collection method for rice stable isotope labeling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106680044A true CN106680044A (en) | 2017-05-17 |
Family
ID=58873005
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611246673.0A Pending CN106680044A (en) | 2016-12-29 | 2016-12-29 | Methane collection device and methane collection method for rice stable isotope labeling |
Country Status (1)
| Country | Link |
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| CN (1) | CN106680044A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107942036A (en) * | 2017-11-16 | 2018-04-20 | 中国科学院亚热带农业生态研究所 | A kind of method and device of isotope labeled in situ Methane in Soils oxidation bacteria |
| CN107966525A (en) * | 2017-11-06 | 2018-04-27 | 中国矿业大学(北京) | It is a kind of13CO2The method of quantification impulse labeled plant |
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