CN207351769U - A kind of soil incubation and gas discharge sampler - Google Patents

A kind of soil incubation and gas discharge sampler Download PDF

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Publication number
CN207351769U
CN207351769U CN201721478196.0U CN201721478196U CN207351769U CN 207351769 U CN207351769 U CN 207351769U CN 201721478196 U CN201721478196 U CN 201721478196U CN 207351769 U CN207351769 U CN 207351769U
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China
Prior art keywords
incubator
sampler
gas
sealing
gas discharge
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Expired - Fee Related
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CN201721478196.0U
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Chinese (zh)
Inventor
张令
邓邦良
王书丽
李超
李晓东
邓文平
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Jiangxi Agricultural University
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Jiangxi Agricultural University
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Abstract

The utility model discloses a kind of soil incubation and gas discharge sampler, including syringe, sealing-plug, incubator and gas sampler, the sealing-plug is installed on the top of incubator, sealing-plug is provided with holes, it is respectively used to by gas sampler and exhaust pipe, gas sampler top is connected by triple valve with syringe, the other end of gas sampler is located inside incubator, gas sampler connects taper tubule for straight-run of pipe lower end and forms, the lower end of exhaust pipe is located inside incubator, upper end is located at outside incubator, and the upper end of exhaust pipe is sealed by two-port valve.The utility model uses combined type incubator, using perforated sectional pipe design sample sampling instrument, can ensure the completeness of ventilation and the representativeness of sampling, improve the accuracy of result of study to greatest extent.

Description

A kind of soil incubation and gas discharge sampler
Technical field
A kind of sampler is the utility model is related to, is specifically a kind of soil incubation and gas discharge sampler.
Background technology
Atmospheric greenhouse gas concentration increase is the Tough questions that current mankind society faces, and greenhouse gases can cause greenhouse Effect, and then cause global warming.And soil is greenhouse gases primary discharge source, how to suppress Soil Trace Gases discharge and then Mitigation of climate change is a major challenge that scientific circles face.Meanwhile the feedback of Soil Trace Gases under climate change environment is imitated Further research, which should be carried out, to be helped to be predicted soil material circulation pattern under Future Climate Change background, is had important Theoretical and realistic meaning.Carbon dioxide, methane and nitrous oxide are three kinds of main greenhouse gas in air, currently to three kinds of greenhouses The Study on Emission of gas mainly includes two methods of the in-situ observation of greenhouse gases and indoor culture studies.Seen relative to original position Survey, on the one hand indoor culture more can effectively control some envirment factors, and then precisely study other envirment factors to soil The influence of earth greenhouse gas emission.Thus, indoor soil incubation be also important means indispensable in current scientific research it One.
The indoor culture of soil is mainly placed in conical flask or other containers using constant incubator control temperature, pedotheque In, one is placed in setting the constant incubator of cultivation temperature.Soil Trace Gases emission monitoring can be by certain time The gaseous sample in closed container is gathered within section, is contained by specific greenhouse gases in gas chromatography analysis gaseous sample Amount, is changed with time by gas content to calculate rate of discharge.Soil incubation and the perfect condition of gas sampling are sealings Ventilation is good in preceding incubator, and gas mixture ratio is more uniform after sealing, and the sample obtained in sample collection is representative, energy Enough distribution situation of the image study gas in container.But in practical study, gaseous sample mainly passes through in culture vessel Syringe connection sampling pipe is manually or by suspension built-in fans mixing, and manually air blast is taken a breath, to a certain degree On internal gas or culture sample are interfered(Such as temperature caused by fan electromotor fever raises, sample surfaces structure By flow perturbation etc.), and in terms of mixed effect there are it is certain the defects of.Meanwhile sample collection mainly passes through one end open Straight tube complete, main collection accumulates in the larger sample of incubator bottom density, thus sample collection representativeness is not good enough, to grinding There is the influence that can not ignore in the accuracy for studying carefully result.Similar research is such as:Deng Bangliang etc., Increases in soil CO2 and N2O emissions with warming depend on plant species in restored Alpine meadows of Wugong Mountain, China, 2016, Journal of Soils and Sediments, 16(3):777-784.The research of Deng Bangliang etc. carries out sample mixing and sampling only with the air collecting pipe of one end open, Disturbed in the presence of to culture sample, gas mixing is uneven, the possibility of the representative deficiency of gas sampling.
Utility model content
The purpose of this utility model is to provide a kind of soil incubation and gas discharge sampler, to solve above-mentioned background skill The problem of being proposed in art.
To achieve the above object, the utility model provides following technical solution:
A kind of soil incubation and gas discharge sampler, including syringe, sealing-plug, incubator and gas sampler, described Sealing-plug is installed on the top of incubator, and sealing-plug is provided with holes, is respectively used to by gas sampler and exhaust pipe, described Gas sampler top is connected by triple valve with syringe, and the other end of gas sampler is located inside incubator, gas sampling Device connects taper tubule for straight-run of pipe lower end and forms, and the lower end of the exhaust pipe is located inside incubator, and upper end is located at Outside incubator, and the upper end of exhaust pipe is sealed by two-port valve.
Further, the incubator has conical flask shape, and incubator is by the top of incubator and incubator bottom two It is grouped into, at the top of incubator and incubator bottom is sealed by incubator to be threadedly coupled and seal.
Further, the outside of incubator bottom is arranged at the top of the incubator of the incubator in threaded connection place.
Further, screw thread, buckle or mill are passed through with incubator bottom two parts at the top of the incubator of the incubator Sand face, which screws, closely to be connected.
Further, the sealing-plug is rubber stopper.
Further, equally distributed stomata is set on the gas sampler, three sections of branches being connected with top straight tube Stomata is in spiral distribution on straight tube, and stomata is distributed in annulus on the annulus tubule of bottom, and towards periphery.
Further, the syringe is sterilized medical syringe, passes through triple valve and gas sampler and gaseous sample Bag is connected.
Further, the exhaust pipe is the glass tubule of both ends open.
Compared with prior art, the beneficial effects of the utility model are:Incubator is made of upper and lower two parts, and opening is Sample can be placed, solves the problems, such as that current culture sample is placed into during incubator and easily attaches incubator inner wall;At the same time Incubator can be opened and carry out open ventilation, or open exhaust pipe and taken a breath by syringe, solve in incubation and be not easy The problem thoroughly ventilated;On the other hand, gas sampler possesses the conical form of multiholed catheter composition, can be to gas in incubator Body is sufficiently mixed, while ensures that gaseous sample collection is representative, ensures the accuracy of result of study.
Brief description of the drawings
Fig. 1 is the structure diagram of the utility model.
In figure:1st, syringe;2nd, triple valve;3rd, gas sampler;4th, two-port valve;5th, exhaust pipe;6th, sealing-plug;7th, cultivate Device;8th, at the top of incubator;9th, incubator bottom;10th, incubator sealing screw thread;11st, culture sample;12nd, gaseous sample bag.
Embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are without making creative work All other embodiments obtained, shall fall within the protection scope of the present invention.
Referring to Fig. 1, in the utility model embodiment, a kind of soil incubation and gas discharge sampler, its structure includes Syringe 1, sealing-plug 6, incubator 7 and gas sampler 3, the incubator 7 have conical flask shape, are adapted for sample culturing And gas collecting, the incubator 7 are made of at the top of incubator 8 and 9 two parts of incubator bottom, incubator top 8 and culture Device bottom 9 is connected and is sealed by incubator sealing screw thread 10, and incubator can be positioned over by culture sample 11 by opening incubator 7 Bottom 9;The sealing-plug 6 is installed on the top of incubator 7, and sealing-plug 6 is rubber stopper, and sealing-plug 6 is provided with holes, uses respectively It is connected in by gas sampler 3 and exhaust pipe 5,3 top of gas sampler by triple valve 2 with syringe 1, syringe 1 is Injector for medical purpose, the other end of gas sampler 3 are located inside incubator 7, and the gas sampler 3 connects for straight-run of pipe lower end Taper tubule composition is connect, the exhaust pipe 5 is the glass tubule of both ends open, and the lower end of exhaust pipe 5 is located in incubator 7 Portion, upper end is located at outside incubator 7, and the upper end of exhaust pipe 5 is sealed by two-port valve 4.
Further, the incubator top 8 of the incubator 7 is arranged at the outside of incubator bottom 9 in threaded connection place, So that the incubator bottom 9 of incubator 7 is contained within 8 at the top of incubator, two parts connection is avoided to influence sample, incubator 7 The flat no concave-convex of inner wall, does not influence internal volume after sealed connection.
Further, the incubator 7 is made of at the top of incubator 8 and 9 two parts of incubator bottom, and two parts can lead to Cross screw thread, buckle or frosting and screw and closely connected;The incubator 7 and gas sampler 3 can be according to culture samples Number it is sized, and determine incubator 7 volume.
Further, the sealing-plug 6 has elasticity, easily punching, its periphery, Ke Yijing more bigger than 7 mouthfuls of internal diameters of incubator Really sealing incubator 7.
Further, equally distributed stomata is set on the gas sampler 3, be convenient for gas be thoroughly mixed and Representative sampling is carried out, stomata layout and direction are set according to culture sample, three sections to be generally connected with top straight tube Stomata is in spiral distribution on branch's straight tube, and stomata is distributed in annulus on the annulus tubule of bottom, towards periphery, ensures uniformly mixing training Gaseous sample in device 7 is supported, while avoids blow-through culture sample 11.
Further, the syringe 1 is sterilized medical syringe, passes through triple valve 2 and gas sampler 3 and gas sample Product bag 12 is connected.
In a preferred embodiment, open incubator 7 to ventilate, syringe 1 connects sampler 3 and sampled.Specifically Operate and be:Incubator 7 is opened, culture sample 11 is placed in incubator bottom 9, incubator top 8 and incubator bottom 9 are by close Envelope screw thread 10 is tightly connected, and opens triple valve 2, keeps the inside of incubator 7 to be connected with outside, while open two-port valve 4 to make exhaust Pipe 5 is unimpeded, and adjusts to required environmental condition and carry out aerobic culture;Rotatory sealing screw thread 10, opens the ventilation of incubator 7 0.5-1 Minute, incubator sealing screw thread 10 is screwed afterwards and seals incubator 7, closes triple valve 2 and two-port valve 4, starts timing, culture dimension Hold 2 it is small when;2 it is small when after, regulating three-way valve 2, keep syringe 1 only connected with sampler 3, pass through 1 air-breathing of syringe and exhaust mix Gaseous sample 2-3 times in incubator 7, then gathers gaseous sample 10ml, disconnects syringe 1 and is connected with incubator 7, regulating three-way valve 2, keep syringe 1 only to be connected with gaseous sample bag 12, gaseous sample is injected into sample sack 12, send survey;Complete a gas collecting Work, repeats above operation, and starts next round culture and gas collecting.
In a preferred embodiment, closed incubator 7, connects sampler 3 by syringe 1, coordinates exhaust pipe 5 to be led to Gas, then connects sampler 3 by syringe 1 and is sampled.Concrete operations are:Incubator 7 is opened, is placed in incubator bottom 9 Culture sample 11, incubator top 8 and incubator bottom 9 are tightly connected by sealing screw thread 10, are opened triple valve 2, are kept training The inside of device 7 is supported to connect with outside, while opening two-port valve 4 makes exhaust pipe 5 unimpeded, and adjust aerobic to the progress of required environmental condition Culture.Regulating three-way valve 2 closes incubator 7 at sampler 3, while makes syringe and air communication, and extracts air 200ml, Subsequent regulating three-way valve 2, makes incubator 7 only be connected with syringe 1, air in syringe 1 is slowly injected incubator 7, in incubator 7 Excessive gas will be discharged by exhaust pipe 5.Repeat above ventilation operation 3 times, afterwards regulating three-way valve 2 and two-port valve 4, keep training It is closed to support device 7, continues to cultivate, when maintenance 2 is small.2 it is small when after, regulating three-way valve 2, keep syringe 1 only connect with sampler 3 It is logical, gaseous sample 2-3 times in device 7 is mixed by 1 air-breathing of syringe and exhaust, gaseous sample 10ml is then gathered, disconnects pin Cylinder 1 is connected with incubator 7, regulating three-way valve 2, keeps syringe 1 only to be connected with gaseous sample bag 12, gaseous sample is injected sample Bag 12, send survey.A gas collecting job is completed, repeats above operation, starts next round culture and gas collecting.
The advantages of the design and innovation:Incubator 7 is made of upper and lower two parts, and opening can place sample, is solved Current culture sample is placed into during incubator 7 the problem of easily attaching incubator inner wall;Incubator 7 can be opened at the same time Open ventilation is carried out, or opens exhaust pipe 5 and is taken a breath by syringe 1, solves the difficulty for being not easy thoroughly to ventilate in incubation Topic;On the other hand, gas sampler 3 possesses the conical form of multiholed catheter composition, gas in incubator 7 can be carried out abundant Mixing, while ensure that gaseous sample collection is representative, ensure the accuracy of result of study.
More than be only the utility model preferred embodiment, it is noted that for those skilled in the art, Without departing from the concept of the premise utility, several modifications and improvements can also be made, these should also be considered as this practicality New protection domain, these are all without the effect and practical applicability for influencing the utility model implementation.

Claims (8)

1. a kind of soil incubation and gas discharge sampler, including syringe(1), sealing-plug(6), incubator(7)And gas sampling Device(3), it is characterised in that the sealing-plug(6)It is installed on incubator(7)Top, sealing-plug(6)Holes is provided with, respectively For passing through gas sampler(3)And exhaust pipe(5), the gas sampler(3)Top passes through triple valve(2)With syringe(1) Connection, gas sampler(3)The other end be located at incubator(7)Inside, gas sampler(3)Connect and bore for straight-run of pipe lower end Shape tubule is formed, the exhaust pipe(5)Lower end be located at incubator(7)Inside, upper end are located at incubator(7)Outside, and Exhaust pipe(5)Upper end pass through two-port valve(4)Sealing.
2. soil incubation according to claim 1 and gas discharge sampler, it is characterised in that the incubator(7)Tool There are conical flask shape, incubator(7)By at the top of incubator(8)With incubator bottom(9)Two parts form, at the top of incubator(8) With incubator bottom(9)Screw thread is sealed by incubator(10)Connect and seal.
3. soil incubation according to claim 1 or 2 and gas discharge sampler, it is characterised in that the incubator(7) Incubator at the top of(8)Incubator bottom is arranged in threaded connection place(9)Outside.
4. soil incubation according to claim 3 and gas discharge sampler, it is characterised in that the incubator(7)'s At the top of incubator(8)With incubator bottom(9)Two parts are screwed by screw thread, buckle or frosting and closely connected.
5. soil incubation according to claim 1 and gas discharge sampler, it is characterised in that the sealing-plug(6)For Rubber stopper.
6. soil incubation according to claim 1 and gas discharge sampler, it is characterised in that the gas sampler (3)Upper to set equally distributed stomata, stomata is in spiral distribution on three sections of branch's straight tubes being connected with top straight tube, bottom annulus Stomata is distributed in annulus on tubule, and towards periphery.
7. soil incubation according to claim 1 and gas discharge sampler, it is characterised in that the syringe(1)For nothing Bacterium injector for medical purpose, passes through triple valve(2)With gas sampler(3)And gaseous sample bag(12)It is connected.
8. soil incubation according to claim 1 and gas discharge sampler, it is characterised in that the exhaust pipe(5)For The glass tubule of both ends open.
CN201721478196.0U 2017-11-08 2017-11-08 A kind of soil incubation and gas discharge sampler Expired - Fee Related CN207351769U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106932241A (en) * 2017-02-09 2017-07-07 山东省农业科学院农业资源与环境研究所 A kind of method of culture soil greenhouse gas emission in measuring cell
CN109238807A (en) * 2018-11-14 2019-01-18 南京信息工程大学 Soil incubation and high-resolution original position gas-liquid sampling apparatus and its test method
CN109298119A (en) * 2018-11-15 2019-02-01 盐城工学院 A kind of simply self-priming headspace sampling pretreating device and its processing method
CN112129889A (en) * 2020-07-31 2020-12-25 南京师范大学 Outdoor determination of plant withered CO2Method of releasing rate

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106932241A (en) * 2017-02-09 2017-07-07 山东省农业科学院农业资源与环境研究所 A kind of method of culture soil greenhouse gas emission in measuring cell
CN106932241B (en) * 2017-02-09 2019-10-08 山东省农业科学院农业资源与环境研究所 A kind of method of culture soil greenhouse gas emission in measuring cell
CN109238807A (en) * 2018-11-14 2019-01-18 南京信息工程大学 Soil incubation and high-resolution original position gas-liquid sampling apparatus and its test method
CN109298119A (en) * 2018-11-15 2019-02-01 盐城工学院 A kind of simply self-priming headspace sampling pretreating device and its processing method
CN112129889A (en) * 2020-07-31 2020-12-25 南京师范大学 Outdoor determination of plant withered CO2Method of releasing rate

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Granted publication date: 20180511

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