CN110274790A - A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method - Google Patents
A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1031—Sampling from special places
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1445—Overpressure, pressurisation at sampling point
- G01N2001/1463—Injector; Air-lift
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
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Abstract
This application discloses a kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application methods, including deposit culture apparatus, gas sampling assembly, liquid sampler, it is a kind of collection Anaerobic culturel, gas collecting, the multi-functional device in one of liquid acquisition, air-tightness is good, the fine Simulated Wetland ecosystem of energy, other than it can carry out Anaerobic culturel, also anaerobic state is able to maintain in sampling process in bottle, not by external interference, accuracy is high, the stratified sampling of gas and liquid can be carried out to deposit simultaneously, resolution ratio is higher, it is small in size, it is light-weight, it is easy to remove, multiple groups can be carried out simultaneously repeats experiment or control experiment, to improve the reliability of experimental data, device production method is easy, it is at low cost, only common Xiao Te bottles need to be slightly transformed, raw material needed for transformation are General experimental material, purchase can be facilitated.
Description
Technical field
The invention belongs to environmental science and ecological study technical field, in particular to deposit Anaerobic culturel and gas-liquid is adopted
Sampling device and its application method.
Background technique
Wetland refers to permanent or seasonal ponding, and grows the area of wetland ecotourism, it is one kind somewhere between aquatic
The ecosystem of the ecosystem and terriecosystems.Although Wetland Area only accounts for the 6% of land surface product on the earth, because of it
With water conservation, regulate the climate, the ecological functions that degradation of contaminant etc. is important and obtain the good reputation of " kidney of the earth ".In general,
Since wetland is in the anaerobic state of waterflooding, inorganic salt content and hole water conductivity are low, have accumulated a large amount of organic carbons, therefore wet
Ground is also the important carbon storehouse of terrestrial ecosystems.However, outside environmental elements become due to the influence of climate change and mankind's activity
Change the decomposition that can significantly affect organic carbon in Wetland Sediments.Therefore, waterflooding under a kind of both analog wetland natural environments is utilized
Anaerobic state, and analog external environment changes, and such as the input of nutrient, the variation of temperature, pH condition, arranges wetland carbon
The culture apparatus impacted is put, is conducive to the changing rule of wetland carbon emission under research environment variation background, is followed for Global Carbon
The calculating of ring provides data supporting.
Currently, there are many cultures and sampling apparatus for deposit or soil, such as: have directly using syringe from culture
Gas is extracted in bottle, has the additional PVC cover of culture bottle, is evacuated by rechargeable circulating pump, has and detested using condensation diffusion principle
Oxygen culture simultaneously samples, but their function is all relatively simple, is not able to satisfy under anaerobic culture conditions gas in situ and liquid-like
The purpose that product are collected, as the Publication No. CN202814959U syringe-type proposed vacuumizes soil anaerobic/oxygen consumption culture bottle can be with
The anaerobic environment of preferable Simulated Wetland deposit, but its gaseous sample that can only extract headspace in bottle, cannot be real simultaneously
The acquisition of gas and fluid sample is carried out at present different depth；A kind of for another example simulation that Publication No. CN106248909A is proposed
The monitoring device of wetlands ecosystems greenhouse gases, not only analog wetland and the external world carried out the process of exchanges of mass and energy, but also
It can be set to the carbon emission of leakproofness system research wetland, but its sampling for pertaining only to gas, liquid do not sampled, suddenly
The variation for having omited the water body chemical ingredient of wetlands ecosystems is also an important factor for influencing carbon emission.In addition, although some are filled
The sampling that can be carried out gas and liquid is set, but structure is complicated, volume is larger inconvenient mobile and at high cost, is not suitable for carrying out a large amount of
Repeat test, as Publication No. CN207570848U propose Sediment Pore Water and dissolved gas acquisition device, although it is obtained
The test data precision obtained is higher, but its required vacuum generator, sample receptacle, gas fixator etc. must pass through
Conduit connection, when use, is unable to warpage, and filtering head will keep certain shape, cannot lose, it is seen that the use of the device
It is more demanding.
Generally speaking, in conjunction with current various cultures and sampling apparatus, be primarily present following deficiency: 1, air-tightness is poor, no
Can keep anaerobic state in entire culture and sampling process, 2, precision it is lower, air-liquid sample in situ cannot be carried out in different depth
It collects；3, volume is larger, and inconvenience carries and uses, and at high cost.
Summary of the invention
The technical issues of solution: the application mainly proposes a kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its makes
With method, the technical problems such as air-tightness existing in the prior art is poor, precision is lower, volume is larger are solved.
Technical solution:
A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus, by deposit culture apparatus, gas sampling assembly and liquid sample
Device composition, the deposit culture apparatus are Xiao Te bottles, are set on Xiao Te bottles of bottle screw cap there are two venthole, two are ventilated
Hole connects a ventilation T-type triple valve with butyl rubber plug respectively, is symmetrical arranged from bottom to top in Xiao Te bottles of body vertical direction
Two column thieff hatch, wherein a column thieff hatch is gas sampling assembly thieff hatch, another column thieff hatch is liquid sampler sampling
Hole, is respectively used to installation gas sampling assembly and liquid sampler, gas sampling assembly include gas sampling T-type triple valve,
Gas sampling PU pipe punches gas sampling hollow rubber plug, the plastics pagoda, silicone tube for keeping its hollow in upper and lower two planes
With solid rubber plug, the gas sampling T-type triple valve, gas sampling PU pipe, gas sampling hollow rubber plug, plastics pagoda,
Silicone tube and solid rubber plug are sequentially connected, and the gas sampling hollow rubber stoppers in gas sampling assembly thieff hatch,
Plastics pagoda, silicone tube and solid rubber plug are located in Xiao Te bottles, and the gas sampling T-type triple valve and gas sampling PU pipe are set
Outside Xiao Te bottles of bottles, liquid sampler includes liquid sample T-type triple valve, Rule female connector, liquid sample PU pipe, Rule public affairs
Connector, soil moisture sampler, the liquid sample T-type triple valve, Rule female connector, liquid sample PU pipe, Rule pin end
It is sequentially connected with soil moisture sampler, takes and stoppered in the liquid sample hollow rubber that the punching of upper and lower two planes keeps its hollow
It fills in liquid sampler thieff hatch, the end of soil moisture sampler penetrates in liquid sample hollow rubber plug, makes liquid
Sampling hollow rubber plug is closely connect with soil moisture sampler, liquid sample T-type triple valve, Rule female connector, liquid sample
PU pipe and Rule pin end are set to outside Xiao Te bottles of bottles.
As a preferred technical solution of the present invention: Xiao Te bottles of the base diameter is 10 cm, Xiao Te bottle height 30
cm。
As a preferred technical solution of the present invention: venthole there are two being set on Xiao Te bottles of the bottle screw cap, two
A venthole is sealed with butyl rubber plug respectively, and passing through two plane punchings of butyl rubber plug or more with punch keeps its hollow, butyl
Rubber plug upper end connection ventilation T-type triple valve.
As a preferred technical solution of the present invention: it is described with electric hand drill Xiao Te bottles of body vertical direction from bottom to top
Symmetrical punching is using as thieff hatch, wherein a column thieff hatch is gas sampling assembly thieff hatch, another column thieff hatch is adopted for liquid
Sampling device thieff hatch.
As a preferred technical solution of the present invention: the thieff hatch of the Xiao Te bottles of bottommost is apart from 5 cm of bottom of bottle, together
Interval between the one two neighboring thieff hatch of column is also 5 cm, and each column has 5 thieff hatch respectively.
As a preferred technical solution of the present invention: the gas sampling T-type triple valve is connected with gas sampling PU pipe,
Gas sampling hollow rubber plug connects gas sampling PU pipe and plastics pagoda, and silicone tube connects plastics pagoda and solid rubber plug,
Solid rubber is stoppered in the end of silicone tube, it is ensured that liquid cannot penetrate into gas producing device.
As a preferred technical solution of the present invention: the gas sampling assembly is along solid rubber plug from Xiao Te bottles of gas
Outside sampler body device thieff hatch in insertion bottle, until gas sampling hollow rubber stoppers gas sampling assembly thieff hatch.
As a preferred technical solution of the present invention: it is hollow that the end of the soil moisture sampler penetrates liquid sample
In rubber stopper, the head of soil moisture sampler and liquid sample hollow rubber stopper close connection, by liquid sampler along soil
Earth moisture sampler end is inserted into bottle outside the liquid sampler thieff hatch on Xiao Te bottle, until the hollow rubber of liquid sample
Rubber plug tightly fills in liquid sampler thieff hatch.
As a preferred technical solution of the present invention: all to connect with Xiao Te bottles in the deposit incubation
Ventilation T-type triple valve, gas sampling T-type triple valve and liquid sample T-type triple valve remain off, ventilation T-type triple valve,
Syringe is equipped on gas sampling T-type triple valve and liquid sample T-type triple valve；The junction of bottle screw cap and butyl rubber plug is set
There is a glass cement sealing, all gas sampling hollow rubber plug, liquid sample hollow rubber plug and Xiao Te bottles of junction are equipped with
Glass cement sealing.
The application method of a kind of deposit Anaerobic culturel and gas-liquid sampling apparatus, specifically comprises the following steps:
Step 1: checking that the junction of bottle screw cap and butyl rubber plug, all gas sampling hollow rubber plug, liquid sample are hollow
Rubber stopper is sealed with glass cement with Xiao Te bottles of junction, and carries out oxygen leakage test, it is ensured that is occurred without leakage, is sunk
Product object is transferred in Xiao Te bottles after completing sampling, to be layered and is put into bottle during attention mobility, avoid silicone tube and the soil water
Divide the liquid sucking tube bending of sampler end；
Step 2: can open the carbon emission of Wetland Sediments under research oxygen free condition by two of bottle screw cap connection
Ventilation T-type triple valve, closes the gas sampling T-type triple valve connecting with gas collecting device and liquid acquisition device and liquid is adopted
Sample T-type triple valve, the ventilation T-type triple valve being connect by one of them with bottle screw cap continuous inflated with nitrogen 60 in Xiao Te bottles divide
Clock makes to become anaerobic state in bottle, is then turned off the two ventilation T-type triple valves connected by bottle screw cap；
Step 3: opening the syringe that deionized water has been drawn in one of ventilation T-type triple valve connection, deionized water is injected, is used
To ensure that deposit is in anaerobic state；
It is all with Xiao Te bottles of the ventilation T-type triple valves connecting, gas sampling T-type triple valves step 4: in deposit incubation
It is remained off with liquid sample T-type triple valve, to guarantee that gas is not handed over ambient atmos in bottle in incubation
It changes；After cultivating 2 weeks, syringe is connect with gas sampling T-type triple valve or liquid sample T-type triple valve, then opens gas and adopt
Sample T-type triple valve or liquid sample T-type triple valve are acquired, and can acquire gas or fluid sample；
Step 5: to study the deposit carbon emission rule under strong reducing condition or general reducing condition, by above-mentioned nitrogen
It is substituted for oxygen or air；To influence of the research water level to deposit carbon emission, by inject the amount of deionized water come
Adjust the height of water level；Influence to research temperature to deposit, is placed in constant temperature and humidity culture for entire culture apparatus
Case；To be protected from light to sample, in Xiao Te bottles of outer package masking foils.
The utility model has the advantages that herein described deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method use the above skill
Art scheme compared with prior art, has following technical effect that
1, the application is to integrate the multi-functional device of Anaerobic culturel, gas collecting, liquid acquisition.
2, air-tightness is good, the fine Simulated Wetland ecosystem of energy, other than it can carry out Anaerobic culturel, in sampling process in bottle
Also it is able to maintain anaerobic state, not by external interference, accuracy is high.
3, the stratified sampling of gas and liquid can be carried out to deposit simultaneously, resolution ratio is higher.
4, small in size, it is light-weight, it is easy to remove, multiple groups can be carried out simultaneously and repeat experiment or control experiment, to improve experiment
The reliability of data result.
5, production method is easy, at low cost, only common Xiao Te bottles slightly need to be transformed, required raw material, which are transformed, is
General experimental material can facilitate purchase.
Detailed description of the invention
Fig. 1 is a kind of deposit Anaerobic culturel of the application and the structural schematic diagram of gas-liquid sampling apparatus.
Fig. 2 is a kind of gas sampling assembly structural representation of the deposit Anaerobic culturel and gas-liquid sampling apparatus of the application
Figure.
Fig. 3 is a kind of liquid sampler structural representation of the deposit Anaerobic culturel and gas-liquid sampling apparatus of the application
Figure.
Description of symbols: 1, T-type triple valve of ventilating；2, butyl rubber plug；3, bottle screw cap；4, Xiao Te bottles；5, gas is adopted
Sampling device acquires hole；6, syringe；7, liquid sampler acquires hole；51, gas sampling T-type triple valve；52, gas sampling is hollow
Rubber stopper；53, silicone tube；54, gas sampling PU is managed；55, plastics pagoda；56 solid rubber plugs；71, liquid sample T-type threeway
Valve；72, liquid sample PU is managed；73, soil moisture sampler；74, Rule female connector；75, Rule pin end.
Specific embodiment
To further illustrate that each embodiment, the invention patent are provided with attached drawing, these attached drawings are in the invention patent discloses
A part of appearance mainly to illustrate embodiment, and can cooperate the associated description of specification former come the running for explaining embodiment
Reason, cooperation refer to these contents, and those of ordinary skill in the art will be understood that other possible embodiments and the present invention are special
The advantage of benefit, the component in figure is not necessarily to scale, and similar component symbol is conventionally used to indicate similar component.Below
In conjunction with Figure of description, specific embodiments of the present invention will be described in further detail.
Embodiment 1
As shown in Figure 1-3, deposit Anaerobic culturel and gas-liquid sampling apparatus, by deposit culture apparatus, gas sampling assembly and
Liquid sampler composition, the deposit culture apparatus are Xiao Te bottle 4, and the base diameter of the Xiao Te bottle 4 is 10 cm,
Xiao Te bottle 4 is 30 cm high, is set on the bottle screw cap 3 of Xiao Te bottle 4 there are two venthole, two ventholes use butyl rubber plug 2 close respectively
Envelope, passing through about 2 two plane punchings of butyl rubber plug with punch keeps its hollow, the connection ventilation T-type threeway of 2 upper end of butyl rubber plug
Valve 1 uses electric hand drill symmetrically to be punched from bottom to top in 4 body vertical direction of Xiao Te bottle using as thieff hatch, wherein a column thieff hatch
For gas sampling assembly thieff hatch 5, another column thieff hatch is liquid sampler thieff hatch 7, and 4 bottommost of Xiao Te bottle is adopted
Interval of the sample hole between 5 cm of bottom of bottle, the two neighboring thieff hatch of same row is also 5 cm, and each column has 5 thieff hatch respectively,
It is respectively used to installation gas sampling assembly and liquid sampler, gas sampling assembly includes gas sampling T-type triple valve 51, gas
Sampler body PU pipe 54 punches gas sampling hollow rubber plug 52, the plastics pagoda 55, silicon for keeping its hollow in upper and lower two planes
Sebific duct 53 and solid rubber plug 56, the gas sampling T-type triple valve 51, gas sampling PU pipe 54, gas sampling hollow rubber
Plug 52, plastics pagoda 55, silicone tube 53 and solid rubber plug 56 are sequentially connected, the gas sampling hollow rubber plug 52 tightly plug in
In gas sampling assembly thieff hatch 5, plastics pagoda 55, silicone tube 53 and solid rubber plug 56 are located in Xiao Te bottle 4, the gas
Sampling T-type triple valve 51 and gas sampling PU pipe 54 are located at outside 4 bottles of Xiao Te bottle, and liquid sampler includes liquid sample T-type three
Port valve 71, Rule female connector 74, liquid sample PU pipe 72, Rule pin end 75, soil moisture sampler 73, the liquid sample
T-type triple valve 71, Rule female connector 74, liquid sample PU pipe 72, Rule pin end 75 and soil moisture sampler 73 successively connect
It connects, takes and stopper in the liquid sample hollow rubber that the punching of upper and lower two planes keeps its hollow in liquid sampler thieff hatch
In 7, the end of soil moisture sampler 73 is penetrated in liquid sample hollow rubber plug, makes liquid sample hollow rubber plug and soil
Moisture sampler 73 closely connects, and liquid sample T-type triple valve 71, Rule female connector 74, liquid sample PU pipe 72 and Rule are public
Connector 75 is set to outside 4 bottles of Xiao Te bottle.
For gas sampling assembly, gas sampling T-type triple valve 51 and gas sampling PU pipe 54 are connected, and gas sampling is hollow
Rubber stopper 52 connects gas sampling PU pipe 54 and plastics pagoda 55, and silicone tube 53 connects plastics pagoda 55 and solid rubber plug 56,
Solid rubber plug 56 is tightly filled in the end of silicone tube 53, it is ensured that liquid cannot penetrate into gas producing device, and gas sampling assembly is along solid
Rubber stopper 56 is inserted into bottle from outside the gas sampling assembly thieff hatch 5 of Xiao Te bottle 4, until gas sampling hollow rubber plug 52 is tight
Fill in gas sampling assembly thieff hatch 5.
For liquid sampler, the end of soil moisture sampler 73 is penetrated in liquid sample hollow rubber plug, soil
The head of moisture sampler 73 and liquid sample hollow rubber stopper close connection, and liquid sampler 7 is sampled along soil moisture
73 end of device is inserted into bottle outside the liquid sampler thieff hatch on Xiao Te bottle 4, until liquid sample hollow rubber stoppers
Fill in liquid sampler thieff hatch 7.
In deposit incubation, all ventilation T-type triple valves 1 being connect with Xiao Te bottle 4, gas sampling T-type triple valve
51 and liquid sample T-type triple valve 71 remain off, ventilation T-type triple valve 1, gas sampling T-type triple valve 51 and liquid
Syringe 6 is equipped on sampling T-type triple valve 71；The junction of bottle screw cap 3 and butyl rubber plug 2 is sealed equipped with glass cement, is owned
The junction of gas sampling hollow rubber plug 52, liquid sample hollow rubber plug and Xiao Te bottle 4 is equipped with glass cement sealing.
Embodiment 2
As shown in Figure 1-3, deposit Anaerobic culturel and gas-liquid sampling apparatus, by deposit culture apparatus, gas sampling assembly and
Liquid sampler composition, the deposit culture apparatus are Xiao Te bottle 4, and the base diameter of the Xiao Te bottle 4 is 10 cm,
Xiao Te bottle 4 is 30 cm high, is set on the bottle screw cap 3 of Xiao Te bottle 4 there are two venthole, two ventholes use butyl rubber plug 2 close respectively
Envelope, passing through about 2 two plane punchings of butyl rubber plug with punch keeps its hollow, the connection ventilation T-type threeway of 2 upper end of butyl rubber plug
Valve 1 uses electric hand drill symmetrically to be punched from bottom to top in 4 body vertical direction of Xiao Te bottle using as thieff hatch, wherein a column thieff hatch
For gas sampling assembly thieff hatch 5, another column thieff hatch is liquid sampler thieff hatch 7, and 4 bottommost of Xiao Te bottle is adopted
Interval of the sample hole between 5 cm of bottom of bottle, the two neighboring thieff hatch of same row is also 5 cm, and each column has 5 thieff hatch respectively,
It is respectively used to installation gas sampling assembly and liquid sampler, gas sampling assembly includes gas sampling T-type triple valve 51, gas
Sampler body PU pipe 54 punches gas sampling hollow rubber plug 52, the plastics pagoda 55, silicon for keeping its hollow in upper and lower two planes
Sebific duct 53 and solid rubber plug 56, the gas sampling T-type triple valve 51, gas sampling PU pipe 54, gas sampling hollow rubber
Plug 52, plastics pagoda 55, silicone tube 53 and solid rubber plug 56 are sequentially connected, the gas sampling hollow rubber plug 52 tightly plug in
In gas sampling assembly thieff hatch 5, plastics pagoda 55, silicone tube 53 and solid rubber plug 56 are located in Xiao Te bottle 4, the gas
Sampling T-type triple valve 51 and gas sampling PU pipe 54 are located at outside 4 bottles of Xiao Te bottle, and liquid sampler includes liquid sample T-type three
Port valve 71, Rule female connector 74, liquid sample PU pipe 72, Rule pin end 75, soil moisture sampler 73, the liquid sample
T-type triple valve 71, Rule female connector 74, liquid sample PU pipe 72, Rule pin end 75 and soil moisture sampler 73 successively connect
It connects, takes and stopper in the liquid sample hollow rubber that the punching of upper and lower two planes keeps its hollow in liquid sampler thieff hatch
In 7, the end of soil moisture sampler 73 is penetrated in liquid sample hollow rubber plug, makes liquid sample hollow rubber plug and soil
Moisture sampler 73 closely connects, and liquid sample T-type triple valve 71, Rule female connector 74, liquid sample PU pipe 72 and Rule are public
Connector 75 is set to outside 4 bottles of Xiao Te bottle.
For gas sampling assembly, gas sampling T-type triple valve 51 and gas sampling PU pipe 54 are connected, and gas sampling is hollow
Rubber stopper 52 connects gas sampling PU pipe 54 and plastics pagoda 55, and silicone tube 53 connects plastics pagoda 55 and solid rubber plug 56,
Solid rubber plug 56 is tightly filled in the end of silicone tube 53, it is ensured that liquid cannot penetrate into gas producing device, and gas sampling assembly is along solid
Rubber stopper 56 is inserted into bottle from outside the gas sampling assembly thieff hatch 5 of Xiao Te bottle 4, until gas sampling hollow rubber plug 52 is tight
Fill in gas sampling assembly thieff hatch 5.
For liquid sampler, the end of soil moisture sampler 73 is penetrated in liquid sample hollow rubber plug, soil
The head of moisture sampler 73 and liquid sample hollow rubber stopper close connection, and liquid sampler 7 is sampled along soil moisture
73 end of device is inserted into bottle outside the liquid sampler thieff hatch on Xiao Te bottle 4, until liquid sample hollow rubber stoppers
Fill in liquid sampler thieff hatch 7.
In deposit incubation, all ventilation T-type triple valves 1 being connect with Xiao Te bottle 4, gas sampling T-type triple valve
51 and liquid sample T-type triple valve 71 remain off, ventilation T-type triple valve 1, gas sampling T-type triple valve 51 and liquid
Syringe 6 is equipped on sampling T-type triple valve 71；The junction of bottle screw cap 3 and butyl rubber plug 2 is sealed equipped with glass cement, is owned
The junction of gas sampling hollow rubber plug 52, liquid sample hollow rubber plug and Xiao Te bottle 4 is equipped with glass cement sealing.
The application method of the deposit Anaerobic culturel and gas-liquid sampling apparatus, specifically comprises the following steps:
Step 1: checking that the junction of bottle screw cap 3 and butyl rubber plug 2, all gas sample hollow rubber plug 52, liquid sample
Hollow rubber plug and the junction of Xiao Te bottle 4 are sealed with glass cement, and carry out oxygen leakage test, it is ensured that are sent out without leakage
Raw, deposit is transferred in Xiao Te bottle 4 after completing sampling, to be layered and is put into bottle during attention mobility, avoid silicone tube 53
With the liquid sucking tube bending of 73 end of soil moisture sampler；
Step 2: two connected by bottle screw cap 3 can be opened for the carbon emission of Wetland Sediments under research oxygen free condition
Ventilation T-type triple valve 1, closes the gas sampling T-type triple valve 51 and liquid connecting with gas collecting device and liquid acquisition device
Sampler body T-type triple valve 71, the ventilation T-type triple valve 1 being connect by one of them with bottle screw cap 3 are continuous in Xiao Te bottle 4
Inflated with nitrogen 60 minutes, make to become anaerobic state in bottle, is then turned off the two ventilation T-type triple valves 1 connected by bottle screw cap 3；
Step 3: opening one of ventilation T-type triple valve 1 connects the syringe 6 for having drawn deionized water, deionized water is injected,
To ensure that deposit is in anaerobic state；
Step 4: in deposit incubation, all ventilation T-type triple valves 1 being connect with Xiao Te bottle 4, gas sampling T-type threeway
Valve 51 and liquid sample T-type triple valve 71 remain off, to guarantee in incubation that gas is not with ambient atmos in bottle
It swaps；After cultivating 2 weeks, syringe 6 is connect with gas sampling T-type triple valve 51 or liquid sample T-type triple valve 71, then
It opens gas sampling T-type triple valve 51 or liquid sample T-type triple valve 71 is acquired, gas or fluid sample can be acquired；
Step 5: to study the deposit carbon emission rule under strong reducing condition or general reducing condition, by above-mentioned nitrogen
It is substituted for oxygen or air；To influence of the research water level to deposit carbon emission, by inject the amount of deionized water come
Adjust the height of water level；Influence to research temperature to deposit, is placed in constant temperature and humidity culture for entire culture apparatus
Case；To be protected from light to sample, masking foil is wrapped up outside Xiao Te bottle 4.
The above description is only a preferred embodiment of the patent of the present invention, is not intended to limit the invention patent, all at this
Within the spirit and principle of patent of invention, any modification, equivalent replacement, improvement and so on should be included in the invention patent
Protection scope within.
Claims (10)
1. a kind of deposit Anaerobic culturel and gas-liquid sampling apparatus, it is characterised in that: filled by deposit culture apparatus, gas sampling
It sets and is formed with liquid sampler, the deposit culture apparatus is Xiao Te bottles (4), is set on the bottle screw cap (3) of Xiao Te bottles (4)
There are two venthole, two ventholes use butyl rubber plug (2) to connect a ventilation T-type triple valve (1) respectively, Xiao Te bottles (4)
Body vertical direction is symmetrical arranged two column thieff hatch from bottom to top, wherein a column thieff hatch is gas sampling assembly thieff hatch (5),
Another column thieff hatch is liquid sampler thieff hatch (7), is respectively used to installation gas sampling assembly and liquid sampler, gas
Sampler body device include gas sampling T-type triple valve (51), gas sampling PU pipe (54), upper and lower two planes punching make it
Hollow gas sampling hollow rubber plug (52), plastics pagoda (55), silicone tube (53) and solid rubber plug (56), the gas
Sample T-type triple valve (51), gas sampling PU manages (54), gas sampling hollow rubber plug (52), plastics pagoda (55), silicone tube
(53) it is sequentially connected with solid rubber plug (56), tightly plug samples the gas sampling hollow rubber plug (52) in gas sampling assembly
In hole (5), plastics pagoda (55), silicone tube (53) and solid rubber plug (56) are located in Xiao Te bottles (4), the gas sampling T
Type triple valve (51) and gas sampling PU pipe (54) are located at outside Xiao Te bottles of (4) bottles, and liquid sampler includes liquid sample T-type three
Port valve (71), Rule female connector (74), liquid sample PU manage (72), Rule pin end (75), soil moisture sampler (73), institute
State liquid sample T-type triple valve (71), Rule female connector (74), liquid sample PU pipe (72), Rule pin end (75) and soil
Moisture sampler (73) is sequentially connected, and is taken and is stoppered in the liquid sample hollow rubber that the punching of upper and lower two planes keeps its hollow
In liquid sampler thieff hatch (7), the end of soil moisture sampler (73) is penetrated in liquid sample hollow rubber plug, is made
Liquid sample hollow rubber plug is closely connect with soil moisture sampler (73), and liquid sample T-type triple valve (71), Rule mother connect
Head (74), liquid sample PU pipe (72) and Rule pin end (75) are set to outside Xiao Te bottles of (4) bottles.
2. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, it is characterised in that: Xiao Te bottles described
(4) base diameter is 10 cm, Xiao Te bottles (4) high 30 cm.
3. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, it is characterised in that: Xiao Te bottles described
(4) it is set on bottle screw cap (3) there are two venthole, two ventholes use butyl rubber plug (2) to seal respectively, are passed through with punch
Two plane punchings keep its hollow to butyl rubber plug (2) up and down, butyl rubber plug (2) upper end connection ventilation T-type triple valve (1).
4. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, it is characterised in that: described to use electric hand drill
It is symmetrically punched from bottom to top in Xiao Te bottles of (4) body vertical direction using as thieff hatch, wherein a column thieff hatch is gas sampling
Device thieff hatch (5), another column thieff hatch are liquid sampler thieff hatch (7).
5. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, it is characterised in that: Xiao Te bottles described
(4) interval of the thieff hatch of bottommost between 5 cm of bottom of bottle, the two neighboring thieff hatch of same row is also 5 cm, each column difference
There are 5 thieff hatch.
6. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, it is characterised in that: the gas sampling
T-type triple valve (51) and gas sampling PU pipe (54) connection, gas sampling hollow rubber plug (52) connect gas sampling PU pipe
(54) and plastics pagoda (55), silicone tube (53) connect plastics pagoda (55) and solid rubber plug (56), solid rubber plug (56)
End of the tight plug in silicone tube (53), it is ensured that liquid cannot penetrate into gas producing device.
7. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, it is characterised in that: the gas sampling
Device is inserted into bottle along solid rubber plug (56) from outside Xiao Te bottles (4) of gas sampling assembly thieff hatch (5), until gas is adopted
Sample hollow rubber plug (52) tightly fills in gas sampling assembly thieff hatch (5).
8. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, it is characterised in that: the soil moisture
The end of sampler (73) penetrates in liquid sample hollow rubber plug, in the head and liquid sample of soil moisture sampler (73)
Empty rubber stopper closely connects, and liquid sampler is adopted along soil moisture sampler (73) end from the liquid on Xiao Te bottles (4)
Outside sampling device thieff hatch (7) in insertion bottle, until liquid sample hollow rubber stoppers liquid sampler thieff hatch (7).
9. deposit Anaerobic culturel according to claim 1 and gas-liquid sampling apparatus, which is characterized in that the deposit training
During supporting, all ventilation T-type triple valves (1) connecting with Xiao Te bottles (4), gas sampling T-type triple valve (51) and liquid are adopted
Sample T-type triple valve (71) remains off, ventilation T-type triple valve (1), gas sampling T-type triple valve (51) and liquid sample T
Syringe (6) are equipped on type triple valve (71)；The junction of bottle screw cap (3) and butyl rubber plug (2) is sealed equipped with glass cement, institute
There is the junction of gas sampling hollow rubber plug (52), liquid sample hollow rubber plug and Xiao Te bottles (4) to be equipped with glass cement close
Envelope.
10. the detection method of a kind of any deposit Anaerobic culturel of claim 1-9 and gas-liquid sampling apparatus, feature exist
In specifically comprising the following steps:
Step 1: checking that the junction of bottle screw cap (3) and butyl rubber plug (2), all gas sample hollow rubber plug (52), liquid
Sampler body hollow rubber plug and the junction of Xiao Te bottles (4) are sealed with glass cement, and carry out oxygen leakage test, it is ensured that
Occur without leakage, deposit is transferred in Xiao Te bottles (4) after completing sampling, to be layered and is put into bottle during attention mobility, be kept away
Exempt from the liquid sucking tube bending of silicone tube (53) and soil moisture sampler (73) end；
Step 2: can open the carbon emission of Wetland Sediments under research oxygen free condition by the two of bottle screw cap (3) connection
A ventilation T-type triple valve (1) closes the gas sampling T-type triple valve connecting with gas collecting device and liquid acquisition device
(51) and liquid sample T-type triple valve (71), the ventilation T-type triple valve (1) being connect by one of them with bottle screw cap (3) are past
It is inflated with nitrogen 60 minutes continuous in Xiao Te bottles (4), make to become anaerobic state in bottle, be then turned off by the two of bottle screw cap (3) connection
A ventilation T-type triple valve (1)；
Step 3: opening the syringe (6) that deionized water has been drawn in one of ventilation T-type triple valve (1) connection, deionization is injected
Water, to ensure that deposit is in anaerobic state；
Step 4: in deposit incubation, all ventilation T-type triple valve (1), gas sampling T-types being connect with Xiao Te bottles (4)
Triple valve (51) and liquid sample T-type triple valve (71) remain off, with guarantee in incubation in bottle gas not with
Ambient atmos swap；After cultivating 2 weeks, by syringe (6) and gas sampling T-type triple valve (51) or liquid sample T-type threeway
Valve (71) connection, then opens gas sampling T-type triple valve (51) or liquid sample T-type triple valve (71) is acquired
Acquire gas or fluid sample；
Step 5: to study the deposit carbon emission rule under strong reducing condition or general reducing condition, by above-mentioned nitrogen
It is substituted for oxygen or air；To influence of the research water level to deposit carbon emission, by inject the amount of deionized water come
Adjust the height of water level；Influence to research temperature to deposit, is placed in constant temperature and humidity culture for entire culture apparatus
Case；To be protected from light to sample, masking foil is wrapped up outside Xiao Te bottles (4).
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| CN201910588178.5A CN110274790A (en) | 2019-07-02 | 2019-07-02 | A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method |
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| CN201910588178.5A CN110274790A (en) | 2019-07-02 | 2019-07-02 | A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111397968A (en) * | 2020-04-22 | 2020-07-10 | 南京信息工程大学 | Device for in-situ collection of pore water and gas in sediment and wetland soil |
| CN111534421A (en) * | 2020-05-14 | 2020-08-14 | 中国科学院沈阳应用生态研究所 | Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device |
| CN118168870A (en) * | 2024-05-13 | 2024-06-11 | 烟台玖樾电力科技有限公司 | Substation monitoring sampling system |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1510682A (en) * | 1974-08-01 | 1978-05-10 | Airco Inc | Blood gas catheter |
| DE19610402A1 (en) * | 1995-08-08 | 1997-02-13 | Kaisergeoconsult Gmbh | Subsoil gas sample collector for e.g. landfill sites - uses gas permeable water=tight pipe that is strengthened, sub=divided into sections, has central gas tapping tube, and can remain in soil |
| CN202814959U (en) * | 2012-08-03 | 2013-03-20 | 中国农业科学院植物保护研究所 | Needle cylinder type vacuum soil anaerobic/aerobic culture bottle and culture system |
| CN103630667A (en) * | 2013-11-19 | 2014-03-12 | 南京信息工程大学 | Device and method for quickly measuring carbon dioxide flux of soil |
| CN205374410U (en) * | 2016-01-15 | 2016-07-06 | 刘峰 | Simulation lake sediment anaerobic cultivation device |
| RU174038U1 (en) * | 2017-04-26 | 2017-09-26 | федеральное государственное бюджетное образовательное учреждение высшего образования "Пермский национальный исследовательский политехнический университет" | LABORATORY INSTALLATION FOR MEASURING THE BIOGAS VOLUME FORMED BY DECOMPOSITION OF ORGANIC MATERIALS UNDER ANAEROBIC CONDITIONS |
| CN108444776A (en) * | 2018-04-08 | 2018-08-24 | 湖北大学 | The sampler and its sampling method of soil column longitudinal direction soil layer gas |
| CN109238807A (en) * | 2018-11-14 | 2019-01-18 | 南京信息工程大学 | Soil incubation and high-resolution original position gas-liquid sampling apparatus and its test method |
| CN109342590A (en) * | 2018-10-29 | 2019-02-15 | 华东师范大学 | A kind of sediments greenhouse gases generate the measurement system of rate |
| CN210741924U (en) * | 2019-07-02 | 2020-06-12 | 南京信息工程大学 | Sediment anaerobic culture and gas-liquid sampling device |
-
2019
- 2019-07-02 CN CN201910588178.5A patent/CN110274790A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1510682A (en) * | 1974-08-01 | 1978-05-10 | Airco Inc | Blood gas catheter |
| DE19610402A1 (en) * | 1995-08-08 | 1997-02-13 | Kaisergeoconsult Gmbh | Subsoil gas sample collector for e.g. landfill sites - uses gas permeable water=tight pipe that is strengthened, sub=divided into sections, has central gas tapping tube, and can remain in soil |
| CN202814959U (en) * | 2012-08-03 | 2013-03-20 | 中国农业科学院植物保护研究所 | Needle cylinder type vacuum soil anaerobic/aerobic culture bottle and culture system |
| CN103630667A (en) * | 2013-11-19 | 2014-03-12 | 南京信息工程大学 | Device and method for quickly measuring carbon dioxide flux of soil |
| CN205374410U (en) * | 2016-01-15 | 2016-07-06 | 刘峰 | Simulation lake sediment anaerobic cultivation device |
| RU174038U1 (en) * | 2017-04-26 | 2017-09-26 | федеральное государственное бюджетное образовательное учреждение высшего образования "Пермский национальный исследовательский политехнический университет" | LABORATORY INSTALLATION FOR MEASURING THE BIOGAS VOLUME FORMED BY DECOMPOSITION OF ORGANIC MATERIALS UNDER ANAEROBIC CONDITIONS |
| CN108444776A (en) * | 2018-04-08 | 2018-08-24 | 湖北大学 | The sampler and its sampling method of soil column longitudinal direction soil layer gas |
| CN109342590A (en) * | 2018-10-29 | 2019-02-15 | 华东师范大学 | A kind of sediments greenhouse gases generate the measurement system of rate |
| CN109238807A (en) * | 2018-11-14 | 2019-01-18 | 南京信息工程大学 | Soil incubation and high-resolution original position gas-liquid sampling apparatus and its test method |
| CN210741924U (en) * | 2019-07-02 | 2020-06-12 | 南京信息工程大学 | Sediment anaerobic culture and gas-liquid sampling device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111397968A (en) * | 2020-04-22 | 2020-07-10 | 南京信息工程大学 | Device for in-situ collection of pore water and gas in sediment and wetland soil |
| CN111397968B (en) * | 2020-04-22 | 2022-03-08 | 南京信息工程大学 | Device for in-situ collection of pore water and gas in sediment and wetland soil |
| CN111534421A (en) * | 2020-05-14 | 2020-08-14 | 中国科学院沈阳应用生态研究所 | Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device |
| CN118168870A (en) * | 2024-05-13 | 2024-06-11 | 烟台玖樾电力科技有限公司 | Substation monitoring sampling system |
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