CN210741924U - Sediment anaerobic culture and gas-liquid sampling device - Google Patents
Sediment anaerobic culture and gas-liquid sampling device Download PDFInfo
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- CN210741924U CN210741924U CN201921012996.2U CN201921012996U CN210741924U CN 210741924 U CN210741924 U CN 210741924U CN 201921012996 U CN201921012996 U CN 201921012996U CN 210741924 U CN210741924 U CN 210741924U
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Abstract
The utility model discloses a deposit anaerobism is cultivateed and gas-liquid sampling device, including deposit culture apparatus, gaseous sampling device, liquid sampling device, it cultivates to collect the anaerobism, gaseous collection, liquid collection is multi-functional in the device of an organic whole, the gas tightness is good, can fine simulation wetland ecosystem, except can carrying out the anaerobism cultivation, also can keep anaerobic state in the bottle in the sampling process, do not receive external disturbance, the accuracy is high, can carry out the layering sampling of gas and liquid to the deposit simultaneously, resolution ratio is higher, small, light in weight, be convenient for remove, can carry out multiunit repetition experiment or contrast experiment simultaneously, reliability with the improvement experiment data result, the device manufacturing method is simple and convenient, and is with low costs, only need to add slightly to reform transform ordinary bottle can, reform transform required raw and other materials and be general experimental materials, can conveniently purchase.
Description
Technical Field
The application belongs to the technical field of environmental science and ecological research, and particularly relates to a sediment anaerobic culture and gas-liquid sampling device.
Background
The wetland is a region where water is permanently or seasonally accumulated and wetland organisms grow, and is an ecosystem between an aquatic ecosystem and a terrestrial ecosystem. Although the wetland area on the earth only occupies 6 percent of the surface area of the land, the wetland has important ecological functions of conserving water sources, regulating climate, degrading pollutants and the like, thereby gaining the reputation of the 'kidney of the earth'. Generally, the wetland is in a flooded anaerobic state, the content of inorganic salt and the electric conductivity of pore water are low, and a large amount of organic carbon is accumulated, so the wetland is also an important carbon reservoir of a land ecosystem. However, the decomposition of organic carbon in wetland sediments is significantly affected by changes in external environmental factors due to climate changes and human activities. Therefore, the culture device which can simulate the anaerobic state of flooding in the natural environment of the wetland and can simulate the external environment changes, such as the changes of the input of nutrient elements, the temperature and the pH condition, and influence the carbon emission of the wetland is utilized, the research on the change rule of the carbon emission of the wetland under the background of the environmental change is facilitated, and the data support is provided for the calculation of global carbon circulation.
Currently, there are various culture and sampling devices for sediments or soils, such as: the cylinder type vacuumizing soil anaerobic/oxygen-consuming culture bottle provided with the publication number of CN202814959U can better simulate the anaerobic environment of wetland sediments, but only can extract a gas sample in the headspace of the bottle, and cannot simultaneously collect the gas and liquid samples at different depths; also, for example, a monitoring device for simulating greenhouse gases in a wetland ecosystem, which is proposed in publication No. CN106248909A, can simulate the process of exchanging substances and energy between the wetland and the outside, and can be set as a sealing system for studying carbon emission of the wetland, but only involves gas sampling, does not sample liquid, and ignores the change of chemical components in the water body of the wetland ecosystem, and is also an important factor influencing carbon emission. In addition, although some devices can sample gas and liquid, the device has complex structure, large volume, inconvenient movement and high cost, and is not suitable for repeated experiments, such as a sediment pore water and dissolved gas collecting device proposed by the publication No. CN207570848U, although the precision of the obtained experimental data is high, the required vacuum generator, a sample storage device, a gas fixer and the like are connected through a conduit, cannot be bent when in use, and the filter head needs to keep a certain shape and cannot be damaged, so that the device has high use requirement.
In summary, in combination with the various current culture and sampling devices, the following disadvantages mainly exist: 1. the air tightness is poor, the anaerobic state can not be kept in the whole culture and sampling process, 2, the precision is low, and in-situ gas and liquid sample collection can not be carried out at different depths; 3. the volume is large, the carrying and the use are inconvenient, and the cost is high.
Disclosure of Invention
The technical problem to be solved is as follows: the utility model overcomes exist not enough among the above-mentioned prior art, disclose a deposit anaerobism is cultivateed and gas-liquid sampling device, solve the relatively poor, the precision of gas tightness that exists among the prior art less, the great shortcoming of volume.
The technical scheme is as follows: the utility model provides a deposit anaerobic culture and gas-liquid sampling device comprises deposit culture apparatus, gaseous sampling device and liquid sampling device, deposit culture apparatus is the schottky bottle, is equipped with two air vents on the screw thread bottle lid of schottky bottle, and two air vents are respectively with butyl rubber stopper connection one T type three-way valve of ventilating, and the body vertical direction at the schottky bottle sets up two sampling holes from bottom to top symmetry, and one of them is gaseous sampling device sampling hole, and another row of sampling hole is liquid sampling device sampling hole, is used for installing gaseous sampling device and liquid sampling device respectively, and gaseous sampling device includes gaseous sampling T type three-way valve, gaseous sampling PU pipe, has punched two upper and lower planes and make its hollow gaseous sampling hollow rubber stopper, plastics pagoda, silicone tube and solid rubber buffer, gaseous sampling T type three-way valve, gaseous sampling PU pipe, Gas sampling hollow rubber stopper, plastics pagoda, silicone tube and solid rubber stopper connect gradually, gas sampling hollow rubber stopper is tightly packed in gas sampling device sampling hole, and plastics pagoda, silicone tube and solid rubber stopper are established in the schottky bottle, gas sampling T type three-way valve and gas sampling PU pipe are established outside the schottky bottle, and liquid sampling device includes liquid sampling T type three-way valve, luer female joint, liquid sampling PU pipe, luer male joint, soil moisture sample thief, liquid sampling T type three-way valve, luer female joint, liquid sampling PU pipe, luer male joint and soil moisture sample thief connect gradually, and it makes its hollow liquid sampling hollow rubber stopper tightly packed in liquid sampling device sampling hole to get to punch in two upper and lower planes, and the end of soil moisture sample thief penetrates in the liquid sampling hollow rubber stopper, makes liquid sampling hollow rubber stopper and soil moisture sample thief zonulae occludens, the liquid sampling T-shaped three-way valve, the luer female connector, the liquid sampling PU pipe and the luer male connector are arranged outside the Schottky bottle.
As a preferred technical scheme of the application: the diameter of the bottom of the Schottky bottle is 10cm, and the height of the Schottky bottle is 30 cm.
As a preferred technical scheme of the application: the screw bottle cap of the Schottky bottle is provided with two vent holes, the two vent holes are respectively sealed by a butyl rubber plug, a puncher penetrates through the upper plane and the lower plane of the butyl rubber plug to punch holes so as to enable the butyl rubber plug to be hollow, and the upper end of the butyl rubber plug is connected with a T-shaped three-way valve for ventilation.
As a preferred technical scheme of the application: the hand electric drill is used for symmetrically punching holes from bottom to top in the vertical direction of the Schottky bottle body to serve as sampling holes, wherein one row of the sampling holes are gas sampling device sampling holes, and the other row of the sampling holes are liquid sampling device sampling holes.
As a preferred technical scheme of the application: the sampling hole at the bottommost part of the Schottky bottle is 5 cm away from the bottle bottom, the interval between two adjacent sampling holes in the same row is also 5 cm, and each row is provided with 5 sampling holes.
As a preferred technical scheme of the application: gaseous sampling T type three-way valve and gaseous sampling PU union coupling, gaseous sampling PU pipe and plastics pagoda are connected to gaseous sampling cavity rubber buffer, and plastic pagoda and solid rubber buffer are connected to the silicone tube, and solid rubber stopper is plugged in the end of silicone tube, ensures that liquid can not permeate the gas production device.
As a preferred technical scheme of the application: the gas sampling device is inserted into the bottle from the outside of the sampling hole of the gas sampling device of the Schottky bottle along the solid rubber plug until the hollow rubber plug for gas sampling tightly plugs the sampling hole of the gas sampling device.
As a preferred technical scheme of the application: during the end of soil moisture sample thief penetrated liquid sampling cavity rubber buffer, the head and the liquid sampling cavity rubber buffer zonulae occludens of soil moisture sample thief, insert the liquid sampling device along soil moisture sample thief end from the liquid sampling device sampling hole outside on the schottky bottle in the bottle, until liquid sampling cavity rubber buffer tight stopper liquid sampling device sampling hole.
As a preferred technical scheme of the application: in the sediment culture process, all the ventilation T-shaped three-way valves, the gas sampling T-shaped three-way valves and the liquid sampling T-shaped three-way valves which are connected with the Schottky bottles are kept in a closed state, and needle cylinders are arranged on the ventilation T-shaped three-way valves, the gas sampling T-shaped three-way valves and the liquid sampling T-shaped three-way valves; the junction of screw bottle lid and butyl rubber stopper is equipped with glass and glues sealedly, and all gaseous sampling cavity rubber plugs, the junction of liquid sampling cavity rubber plug and schottky bottle all are equipped with glass and glue sealedly.
Has the advantages that:
1. the device integrates anaerobic culture, gas collection and liquid collection.
2. The air tightness is good, the wetland ecosystem can be well simulated, anaerobic culture can be performed, an anaerobic state can be kept in a bottle in the sampling process, external interference is avoided, and the accuracy is high.
3. The device can simultaneously carry out layered sampling on gas and liquid on the sediment, and has higher resolution.
4. The volume is small, the weight is light, the movement is convenient, and a plurality of groups of repeated experiments or contrast experiments can be carried out simultaneously so as to improve the reliability of the experimental data result.
5. The manufacturing method is simple and convenient, the cost is low, only a small amount of modification is needed for a common Schottky bottle, and raw materials required by modification are general experimental materials and can be purchased conveniently.
Drawings
Fig. 1 is a schematic structural diagram of the device for anaerobic culture of sediment and gas-liquid sampling of the present invention.
FIG. 2 is a schematic structural diagram of a gas sampling device of the sediment anaerobic culture and gas-liquid sampling device.
FIG. 3 is a schematic structural diagram of a liquid sampling device of the sediment anaerobic culture and gas-liquid sampling device.
Description of reference numerals: 1. a T-shaped three-way valve for ventilation; 2. a butyl rubber plug; 3. a screw cap; 4. a Schottky bottle; 5. a gas sampling device collection hole; 6. a needle cylinder; 7. a liquid sampling device collection aperture; 51. a gas sampling T-shaped three-way valve; 52. a gas sampling hollow rubber plug; 53. a silicone tube; 54. gas sampling PU tube; 55. A plastic pagoda; 56 solid rubber plug; 71. a T-shaped three-way valve for liquid sampling; 72. liquid sampling PU pipe; 73. a soil moisture sampler; 74. a luer female connector; 75. luer male connector.
Detailed Description
The following examples are presented to enable one of ordinary skill in the art to more fully understand the present invention, but are not intended to limit the invention in any manner. For further explanation of the embodiments, the drawings are provided as part of the disclosure and serve primarily to illustrate the embodiments and, together with the description, to explain the principles of operation of the embodiments, and to provide further explanation of the invention and advantages thereof, it will be understood by those skilled in the art that various other embodiments and advantages of the invention are possible, and that elements in the drawings are not to scale and that like reference numerals are generally used to designate like elements.
Example 1
As shown in fig. 1-3, the sediment anaerobic culture and gas-liquid sampling device comprises a sediment culture device, a gas sampling device and a liquid sampling device, wherein the sediment culture device is a schottky bottle 4, the bottom diameter of the schottky bottle 4 is 10cm, the height of the schottky bottle 4 is 30cm, two vent holes are arranged on a screw cap 3 of the schottky bottle 4, the two vent holes are respectively sealed by a butyl rubber plug 2, a puncher penetrates through the upper plane and the lower plane of the butyl rubber plug 2 to punch holes to make the butyl rubber plug hollow, the upper end of the butyl rubber plug 2 is connected with a T-shaped three-way valve 1, the schottky bottle 4 is symmetrically punched from bottom to top in the vertical direction of the bottle body by using an electric hand drill to serve as sampling holes, one row of the sampling holes is a gas sampling device sampling hole 5, the other row of the sampling holes is a liquid sampling device sampling hole 7, the sampling hole at the bottommost part of the schottky bottle 4 is 5 cm away, every row has 5 sampling holes respectively, is used for installing gaseous sampling device and liquid sampling device respectively, and gaseous sampling device includes gaseous sampling T type three-way valve 51, gaseous sampling PU pipe 54, has punched at two upper and lower planes and has made its hollow gaseous sampling hollow rubber stopper 52, plastics pagoda 55, silicone tube 53 and solid rubber stopper 56, gaseous sampling T type three-way valve 51, gaseous sampling PU pipe 54, gaseous sampling hollow rubber stopper 52, plastics pagoda 55, silicone tube 53 and solid rubber stopper 56 connect gradually, gaseous sampling hollow rubber stopper 52 tightly plugs in gaseous sampling device sampling hole 5, plastics pagoda 55, silicone tube 53 and solid rubber stopper 56 establish in schottky bottle 4, gaseous sampling T type three-way valve 51 and gaseous sampling PU pipe 54 establish outside schottky bottle 4, and liquid sampling device includes liquid sampling T type three-way valve 71, luer connector 74, luer female joint 74, Liquid sampling PU pipe 72, luer male joint 75, soil moisture sample thief 73, liquid sampling T type three-way valve 71, luer female joint 74, liquid sampling PU pipe 72, luer male joint 75 and soil moisture sample thief 73 connect gradually, get two planes about punch and make its hollow liquid sampling hollow rubber stopper tightly fill in liquid sampling device sampling hole 7, the end of soil moisture sample thief 73 penetrates in the liquid sampling hollow rubber stopper, make liquid sampling hollow rubber stopper and soil moisture sample thief 73 zonulae occludens, liquid sampling T type three-way valve 71, luer female joint 74, liquid sampling PU pipe 72 and luer male joint 75 locate outside schottky bottle 4 bottles.
For the gas sampling device, the gas sampling T-shaped three-way valve 51 is connected with the gas sampling PU pipe 54, the gas sampling hollow rubber plug 52 is connected with the gas sampling PU pipe 54 and the plastic tower 55, the silicone tube 53 is connected with the plastic tower 55 and the solid rubber plug 56, the solid rubber plug 56 is tightly plugged at the tail end of the silicone tube 53 to ensure that liquid cannot permeate into the gas sampling device, and the gas sampling device is inserted into a bottle from the outside of the gas sampling device sampling hole 5 of the Schottky bottle 4 along the solid rubber plug 56 until the gas sampling hollow rubber plug 52 is tightly plugged into the gas sampling device sampling hole 5.
For the liquid sampling device, the tail end of the soil moisture sampler 73 penetrates into the liquid sampling hollow rubber plug, the head of the soil moisture sampler 73 is tightly connected with the liquid sampling hollow rubber plug, and the liquid sampling device 7 is inserted into the schottky bottle 4 from the outside of the liquid sampling device sampling hole along the tail end of the soil moisture sampler 73 until the liquid sampling hollow rubber plug tightly plugs the liquid sampling device sampling hole 7.
In the process of culturing the deposit, all the ventilation T-shaped three-way valve 1, the gas sampling T-shaped three-way valve 51 and the liquid sampling T-shaped three-way valve 71 which are connected with the Schottky bottle 4 are kept in a closed state, and the ventilation T-shaped three-way valve 1, the gas sampling T-shaped three-way valve 51 and the liquid sampling T-shaped three-way valve 71 are all provided with a needle cylinder 6; the junction of the screw bottle cap 3 and the butyl rubber plug 2 is provided with a glass cement seal, and the junctions of all the gas sampling hollow rubber plugs 52, the liquid sampling hollow rubber plugs and the Schottky bottle 4 are provided with glass cement seals.
Example two:
as shown in fig. 1-3, the sediment anaerobic culture and gas-liquid sampling device comprises a sediment culture device, a gas sampling device and a liquid sampling device, wherein the sediment culture device is a schottky bottle 4, the bottom diameter of the schottky bottle 4 is 10cm, the height of the schottky bottle 4 is 30cm, two vent holes are arranged on a screw cap 3 of the schottky bottle 4, the two vent holes are respectively sealed by a butyl rubber plug 2, a puncher penetrates through the upper plane and the lower plane of the butyl rubber plug 2 to punch holes to make the butyl rubber plug hollow, the upper end of the butyl rubber plug 2 is connected with a T-shaped three-way valve 1, the schottky bottle 4 is symmetrically punched from bottom to top in the vertical direction of the bottle body by using an electric hand drill to serve as sampling holes, one row of the sampling holes is a gas sampling device sampling hole 5, the other row of the sampling holes is a liquid sampling device sampling hole 7, the sampling hole at the bottommost part of the schottky bottle 4 is 5 cm away, every row has 5 sampling holes respectively, is used for installing gaseous sampling device and liquid sampling device respectively, and gaseous sampling device includes gaseous sampling T type three-way valve 51, gaseous sampling PU pipe 54, has punched at two upper and lower planes and has made its hollow gaseous sampling hollow rubber stopper 52, plastics pagoda 55, silicone tube 53 and solid rubber stopper 56, gaseous sampling T type three-way valve 51, gaseous sampling PU pipe 54, gaseous sampling hollow rubber stopper 52, plastics pagoda 55, silicone tube 53 and solid rubber stopper 56 connect gradually, gaseous sampling hollow rubber stopper 52 tightly plugs in gaseous sampling device sampling hole 5, plastics pagoda 55, silicone tube 53 and solid rubber stopper 56 establish in schottky bottle 4, gaseous sampling T type three-way valve 51 and gaseous sampling PU pipe 54 establish outside schottky bottle 4, and liquid sampling device includes liquid sampling T type three-way valve 71, luer connector 74, luer female joint 74, Liquid sampling PU pipe 72, luer male joint 75, soil moisture sample thief 73, liquid sampling T type three-way valve 71, luer female joint 74, liquid sampling PU pipe 72, luer male joint 75 and soil moisture sample thief 73 connect gradually, get two planes about punch and make its hollow liquid sampling hollow rubber stopper tightly fill in liquid sampling device sampling hole 7, the end of soil moisture sample thief 73 penetrates in the liquid sampling hollow rubber stopper, make liquid sampling hollow rubber stopper and soil moisture sample thief 73 zonulae occludens, liquid sampling T type three-way valve 71, luer female joint 74, liquid sampling PU pipe 72 and luer male joint 75 locate outside schottky bottle 4 bottles.
For the gas sampling device, the gas sampling T-shaped three-way valve 51 is connected with the gas sampling PU pipe 54, the gas sampling hollow rubber plug 52 is connected with the gas sampling PU pipe 54 and the plastic tower 55, the silicone tube 53 is connected with the plastic tower 55 and the solid rubber plug 56, the solid rubber plug 56 is tightly plugged at the tail end of the silicone tube 53 to ensure that liquid cannot permeate into the gas sampling device, and the gas sampling device is inserted into a bottle from the outside of the gas sampling device sampling hole 5 of the Schottky bottle 4 along the solid rubber plug 56 until the gas sampling hollow rubber plug 52 is tightly plugged into the gas sampling device sampling hole 5.
For the liquid sampling device, the tail end of the soil moisture sampler 73 penetrates into the liquid sampling hollow rubber plug, the head of the soil moisture sampler 73 is tightly connected with the liquid sampling hollow rubber plug, and the liquid sampling device 7 is inserted into the schottky bottle 4 from the outside of the liquid sampling device sampling hole along the tail end of the soil moisture sampler 73 until the liquid sampling hollow rubber plug tightly plugs the liquid sampling device sampling hole 7.
In the process of culturing the deposit, all the ventilation T-shaped three-way valve 1, the gas sampling T-shaped three-way valve 51 and the liquid sampling T-shaped three-way valve 71 which are connected with the Schottky bottle 4 are kept in a closed state, and the ventilation T-shaped three-way valve 1, the gas sampling T-shaped three-way valve 51 and the liquid sampling T-shaped three-way valve 71 are all provided with a needle cylinder 6; the junction of the screw bottle cap 3 and the butyl rubber plug 2 is provided with a glass cement seal, and the junctions of all the gas sampling hollow rubber plugs 52, the liquid sampling hollow rubber plugs and the Schottky bottle 4 are provided with glass cement seals.
The use method of the sediment anaerobic culture and gas-liquid sampling device specifically comprises the following steps:
the first step is as follows: the joints of the screw bottle cap 3 and the butyl rubber plug 2, the joints of all the gas sampling hollow rubber plugs 52 and the liquid sampling hollow rubber plugs and the Schottky bottle 4 are all sealed by glass cement, oxygen leakage test is carried out to ensure that no leakage occurs, the sediments are transferred into the Schottky bottle 4 after sampling is finished, the sediments are placed into the bottle in a layering mode in the transfer process, and the bending of a liquid collecting pipe at the tail end of the silicone tube 53 and the tail end of the soil moisture sampler 73 is avoided;
the second step is that: for researching carbon emission of wetland sediments under an anaerobic condition, two ventilation T-shaped three-way valves 1 connected by a screw cap 3 can be opened, a gas sampling T-shaped three-way valve 51 and a liquid sampling T-shaped three-way valve 71 connected with a gas collecting device and a liquid collecting device are closed, nitrogen is continuously filled into a Schottky bottle 4 for 60 minutes through one ventilation T-shaped three-way valve 1 connected with the screw cap 3, the interior of the Schottky bottle is changed into an anaerobic state, and then the two ventilation T-shaped three-way valves 1 connected by the screw cap 3 are closed;
the third step: opening one of the T-shaped three-way valves 1 to connect the needle cylinder 6 which has absorbed deionized water, and injecting deionized water to ensure that the sediment is in an anaerobic state;
the fourth step: in the sediment culture process, all the ventilation T-shaped three-way valve 1, the gas sampling T-shaped three-way valve 51 and the liquid sampling T-shaped three-way valve 71 which are connected with the Schottky bottle 4 are kept in a closed state, so that the gas in the bottle is not exchanged with the external gas in the culture process; after 2 weeks of culture, connecting the needle cylinder 6 with the gas sampling T-shaped three-way valve 51 or the liquid sampling T-shaped three-way valve 71, and then opening the gas sampling T-shaped three-way valve 51 or the liquid sampling T-shaped three-way valve 71 for collection, so that a gas or liquid sample can be collected;
the fifth step: if the carbon emission rule of the sediment under the strong reduction condition or the general reduction condition needs to be researched, replacing the nitrogen with oxygen or air; if the influence of the water level on the carbon emission of the sediment needs to be researched, the height of the water level can be adjusted by injecting the deionized water; if the influence of temperature on sediments needs to be researched, the whole culture device is placed in a constant-temperature constant-humidity incubator; if the sample is to be processed in a dark place, the schottky barrier 4 is wrapped with a tin foil paper.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The utility model provides a deposit anaerobic culture and gas-liquid sampling device which characterized in that: comprises a deposit culture device, a gas sampling device and a liquid sampling device, wherein the deposit culture device is a Schottky bottle (4), two vent holes are arranged on a screw bottle cap (3) of the Schottky bottle (4), the two vent holes are respectively connected with a T-shaped three-way valve (1) for ventilation by a butyl rubber plug (2), two columns of sampling holes are symmetrically arranged in the vertical direction of a bottle body of the Schottky bottle (4) from bottom to top, one column of the sampling holes are gas sampling device sampling holes (5), the other column of the sampling holes are liquid sampling device sampling holes (7) which are respectively used for installing the gas sampling device and the liquid sampling device, the gas sampling device comprises a gas sampling T-shaped three-way valve (51), a gas sampling PU pipe (54), a hollow gas sampling hollow rubber plug (52) which is punched on an upper plane and a lower plane and is used for punching the hollow gas sampling hollow rubber plug, a plastic pagoda (, gaseous sampling T type three-way valve (51), gaseous sampling PU pipe (54), gaseous sampling cavity rubber buffer (52), plastics pagoda (55), silicone tube (53) and solid rubber buffer (56) connect gradually, gaseous sampling cavity rubber buffer (52) tightly stopper in gaseous sampling device sampling hole (5), and plastics pagoda (55), silicone tube (53) and solid rubber buffer (56) are established in schottky bottle (4), gaseous sampling T type three-way valve (51) and gaseous sampling PU pipe (54) are established outside schottky bottle (4) bottle, and liquid sampling device includes liquid sampling T type three-way valve (71), luer female joint (74), liquid sampling PU pipe (72), luer male joint (75), soil moisture sample thief (73), liquid sampling T type three-way valve (71), luer female joint (74), liquid sampling PU pipe (72), Luer male connector (75) and soil moisture sample thief (73) connect gradually, two planes about having taken punch and make its hollow liquid sampling hollow rubber stopper tightly fill in liquid sampling device sampling hole (7), the end of soil moisture sample thief (73) penetrates in the liquid sampling hollow rubber stopper, make liquid sampling hollow rubber stopper and soil moisture sample thief (73) zonulae occludens, liquid sampling T type three-way valve (71), luer female connects (74), liquid sampling PU pipe (72) and luer male connector (75) locate outside schottky bottle (4) bottle.
2. The sediment anaerobic culture and gas-liquid sampling device according to claim 1, characterized in that: the diameter of the bottom of the Schottky bottle (4) is 10cm, and the height of the Schottky bottle (4) is 30 cm.
3. The sediment anaerobic culture and gas-liquid sampling device according to claim 1, characterized in that: the device is characterized in that two vent holes are formed in a screw bottle cap (3) of the Schottky bottle (4), the two vent holes are respectively sealed by a butyl rubber plug (2), a hole puncher penetrates through the upper plane and the lower plane of the butyl rubber plug (2) to punch holes to enable the butyl rubber plug to be hollow, and the upper end of the butyl rubber plug (2) is connected with a T-shaped three-way valve (1) for ventilation.
4. The sediment anaerobic culture and gas-liquid sampling device according to claim 1, characterized in that: the Schottky bottle (4) body vertical direction from bottom to top symmetry is punched in order to be regarded as the sampling hole, and one of them row of sampling hole is gaseous sampling device sampling hole (5), and another row of sampling hole is liquid sampling device sampling hole (7).
5. The sediment anaerobic culture and gas-liquid sampling device according to claim 1, characterized in that: the sampling hole at the bottommost part of the Schottky bottle (4) is 5 cm away from the bottle bottom, the interval between two adjacent sampling holes in the same row is also 5 cm, and each row is provided with 5 sampling holes.
6. The sediment anaerobic culture and gas-liquid sampling device according to claim 1, characterized in that: the gas sampling T-shaped three-way valve (51) is connected with the gas sampling PU pipe (54), the gas sampling hollow rubber plug (52) is connected with the gas sampling PU pipe (54) and the plastic tower (55), the silicone tube (53) is connected with the plastic tower (55) and the solid rubber plug (56), and the solid rubber plug (56) is tightly plugged at the tail end of the silicone tube (53) to ensure that liquid cannot permeate into the gas production device.
7. The sediment anaerobic culture and gas-liquid sampling device of claim 3, characterized in that: the gas sampling device is inserted into the Schottky bottle (4) from the outside of a gas sampling device sampling hole (5) along a solid rubber plug (56) until a gas sampling hollow rubber plug (52) tightly plugs the gas sampling device sampling hole (5).
8. The sediment anaerobic culture and gas-liquid sampling device according to claim 1, characterized in that: during the end of soil moisture sample thief (73) penetrated liquid sampling cavity rubber buffer, the head and the liquid sampling cavity rubber buffer zonulae occludens of soil moisture sample thief (73), insert liquid sampling device along soil moisture sample thief (73) end from liquid sampling device sampling hole (7) outside on schottky barrier (4) in the bottle until liquid sampling cavity rubber buffer tight plug liquid sampling device sampling hole (7).
9. The sediment anaerobic culture and gas-liquid sampling device according to claim 1, characterized in that: in the sediment culture process, all the ventilation T-shaped three-way valve (1), the gas sampling T-shaped three-way valve (51) and the liquid sampling T-shaped three-way valve (71) which are connected with the Schottky bottle (4) are kept in a closed state, and needle cylinders (6) are arranged on the ventilation T-shaped three-way valve (1), the gas sampling T-shaped three-way valve (51) and the liquid sampling T-shaped three-way valve (71); the junction of the screw bottle cap (3) and the butyl rubber plug (2) is provided with a glass cement seal, and the junctions of all the gas sampling hollow rubber plugs (52), the liquid sampling hollow rubber plugs and the Schottky bottle (4) are provided with glass cement seals.
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| CN201921012996.2U CN210741924U (en) | 2019-07-02 | 2019-07-02 | Sediment anaerobic culture and gas-liquid sampling device |
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| CN201921012996.2U CN210741924U (en) | 2019-07-02 | 2019-07-02 | Sediment anaerobic culture and gas-liquid sampling device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110274790A (en) * | 2019-07-02 | 2019-09-24 | 南京信息工程大学 | A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110274790A (en) * | 2019-07-02 | 2019-09-24 | 南京信息工程大学 | A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method |
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