CN205374410U - Simulation lake sediment anaerobic cultivation device - Google Patents

Simulation lake sediment anaerobic cultivation device Download PDF

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Publication number
CN205374410U
CN205374410U CN201620035783.1U CN201620035783U CN205374410U CN 205374410 U CN205374410 U CN 205374410U CN 201620035783 U CN201620035783 U CN 201620035783U CN 205374410 U CN205374410 U CN 205374410U
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China
Prior art keywords
communicating pipe
external environment
nitrogen
trachea
lake
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Expired - Fee Related
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CN201620035783.1U
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Chinese (zh)
Inventor
刘峰
孙蕾
王静岚
闫喜凤
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Abstract

Simulation lake sediment anaerobic cultivation device, its structure includes anaerobic cultivation bottle, turned welt rubber buffer, is detained the needle, syringe needle transfusion system, trachea shunting ware, intercommunication rubber tube no. 1, train gaseous output tube, with external environment communicating pipe, rubber stopper, erlenmeyer flask, nitrogen gas communicating pipe, nitrogen cylinder, intercommunication rubber tube no. 2. The utility model discloses a when studying lake sediment, simulation lake substrate environment can reduce research equipment cost by a wide margin and last the running cost to satisfy most of requirements of testing basically. Lake bed mud anaerobism and relative light -resistant environment can be fully simulated in the research of the same kind, external environment factor of influence is reduced to keep with pressing steady voltage constant temperature with the external world, experiment medicament reliable and simple takes out in experimentation china and canada, and whole device has great practicality.

Description

Simulation lake sediment anaerobic culture device
Technical field
This utility model relates to a kind of simulation lake sediment anaerobic culture device, is specifically related to a kind of to study the simulation lake sediment anaerobic culture device that in bottom mud in lake deposit, polluter and growth of microorganism design for target.
Background technology
In water environment, having a strong impact on of persistent pollutant constantly receives everybody concern in recent years, the persistent pollutants such as most of PAHs in the water body environments such as river, lake, ocean, organochlorine pesticide are finally all deposited thing and are adsorbed, and deposit is the main environment home to return to of PAHs.So persistent pollutant Study on degradation is focused in the middle of bottom mud in lake or deposit by most of scientific research personnel, therefore imitative bottom mud in lake and Sediment environment become emphasis and the difficult point of research, it is critical only that and at least ensure anaerobism and relative light protected environment, and this kind of research, use anaerobic culture box and anaerobism work station more, the bigger research cost of its equipment investment is too high, one equipment height person reaches hundreds thousand of unit, and experimentation is difficult to keep normal pressure voltage stabilizing with the external world, and the experimental program according to oneself adds medicament and the continuous service of mental retardation low consumption at random.
Summary of the invention
The purpose of this utility model is to provide one simulation lake sediment anaerobic culture device for prior art deficiency.
Anaerobic culture device structure described in the utility model includes Anaerobic culturel bottle, turned welt rubber stopper, is detained pin, syringe needle transfusion system, trachea coupler, connection sebific duct one, cultivate gas output tube and external environment communicating pipe, rubber closure, conical flask, nitrogen communicating pipe, nitrogen cylinder, connect sebific duct two;
Turned welt rubber stopper inserts syringe needle transfusion system and is detained pin, one end of many connection sebific ducts two connects trachea coupler, the other end connects Anaerobic culturel bottle, one end of connection sebific duct one connects trachea coupler, the other end is connected with cultivating gas output tube, cultivates gas output tube and is inserted the nearly bottom of conical flask by rubber closure, and the top of conical flask picks out two pipes by rubber closure, wherein a nitrogen communicating pipe connects nitrogen cylinder, and another root is connect external environment with external environment communicating pipe.
The beneficial effects of the utility model: when the purpose of this utility model patent is that lake sediment is studied, simulation lake substrate environment, it is possible to research equipment expense and continuous service cost are greatly lowered, and meet most of basic experiment requirement.Can fully simulating bottom mud in lake anaerobism and relative light protected environment in similar research, reduce external environment factor of influence, and keep with pressure voltage stabilizing constant temperature with the external world, add that to take out experimental pharmacy simple in experimentation, whole device has bigger practicality.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
1. Anaerobic culturel bottles, 2. turned welt rubber stopper in figure, 3. be detained pin, 4. syringe needle transfusion system, 5. trachea coupler, 6. connection sebific duct one, 7. cultivate gas output tube, 8. with external environment communicating pipe, 9. rubber closure, 10. conical flask, 11. nitrogen communicating pipes, 12. nitrogen cylinders, 13. connect sebific duct two;A is lake sediment C for cultivation liquid B is deionized water.
Detailed description of the invention
Anaerobic culture device structure described in the utility model includes Anaerobic culturel bottle, turned welt rubber stopper, is detained pin, syringe needle transfusion system, trachea coupler, connection sebific duct one, cultivate gas output tube and external environment communicating pipe, rubber closure, conical flask, nitrogen communicating pipe, nitrogen cylinder, connect sebific duct two;
Turned welt rubber stopper inserts syringe needle transfusion system and is detained pin, one end of many connection sebific ducts two connects trachea coupler, the other end connects Anaerobic culturel bottle, one end of connection sebific duct one connects trachea coupler, the other end is connected with cultivating gas output tube, cultivates gas output tube and is inserted the nearly bottom of conical flask by rubber closure, and the top of conical flask is by rubber closure two pipes of contact, wherein a nitrogen communicating pipe connects nitrogen cylinder, and another root is connect external environment with external environment communicating pipe.
Concrete operations example: nitrogen after bottled for multiple Anaerobic culturel good laboratory sample is purged 5-10 minute (make Anaerobic culturel bottle in anaerobic state), and put masking foil parcel body (lucifuge) after turned welt rubber stopper;
Turned welt rubber stopper inserts syringe needle transfusion system and is detained pin (being detained pin to add medicament in the constant situation of the environment such as oxygen amount and pressure in an experiment);Again one end of the connection sebific duct two of many syringe needle transfusion systems being connected trachea coupler, the other end inserts below conical flask liquid level by turned welt rubber stopper, has put into the deionized water about 2/3 volume in bottle;
In conical flask, liquid level separately picks out two pipes (making device voltage stabilizing) by turned welt rubber stopper, a piece nitrogen communicating pipe connects nitrogen cylinder, prevent oxygen from entering (being used for maintaining the interior anaerobic environment relatively of conical flask) for nitrogen mouth blown, another root, for connecting external environment (for device normal pressure voltage stabilizing and prevent liquid from flowing backwards) with external environment communicating pipe, builds the requirement of experiment of the requirements such as same pressure voltage stabilizing anaerobism in maintenance many anaerobic reactions bottle with this.
In the parallel immigration constant temperature control box of whole experimental provision, design temperature, to reach to control in experiment the requirement of temperature.

Claims (1)

1. a simulation lake sediment anaerobic culture device, it is characterised in that: described anaerobic culture device structure includes Anaerobic culturel bottle, turned welt rubber stopper, is detained pin, syringe needle transfusion system, trachea coupler, connection sebific duct one, cultivate gas output tube and external environment communicating pipe, rubber closure, conical flask, nitrogen communicating pipe, nitrogen cylinder, connect sebific duct two;
Turned welt rubber stopper inserts syringe needle transfusion system and is detained pin, one end of many connection sebific ducts two connects trachea coupler, the other end connects Anaerobic culturel bottle, one end of connection sebific duct one connects trachea coupler, the other end is connected with cultivating gas output tube, cultivates gas output tube and is inserted the nearly bottom of conical flask by rubber closure, and the top of conical flask picks out two pipes by rubber closure, wherein a nitrogen communicating pipe connects nitrogen cylinder, and another root is connect external environment with external environment communicating pipe.
CN201620035783.1U 2016-01-15 2016-01-15 Simulation lake sediment anaerobic cultivation device Expired - Fee Related CN205374410U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620035783.1U CN205374410U (en) 2016-01-15 2016-01-15 Simulation lake sediment anaerobic cultivation device

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Application Number Priority Date Filing Date Title
CN201620035783.1U CN205374410U (en) 2016-01-15 2016-01-15 Simulation lake sediment anaerobic cultivation device

Publications (1)

Publication Number Publication Date
CN205374410U true CN205374410U (en) 2016-07-06

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110082484A (en) * 2019-06-04 2019-08-02 中国水产科学研究院黑龙江水产研究所 A kind of water-deposit degradation experiment system
CN110274790A (en) * 2019-07-02 2019-09-24 南京信息工程大学 A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method
CN111534421A (en) * 2020-05-14 2020-08-14 中国科学院沈阳应用生态研究所 Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110082484A (en) * 2019-06-04 2019-08-02 中国水产科学研究院黑龙江水产研究所 A kind of water-deposit degradation experiment system
CN110082484B (en) * 2019-06-04 2024-03-26 中国水产科学研究院黑龙江水产研究所 Water-sediment degradation experiment system
CN110274790A (en) * 2019-07-02 2019-09-24 南京信息工程大学 A kind of deposit Anaerobic culturel and gas-liquid sampling apparatus and its application method
CN111534421A (en) * 2020-05-14 2020-08-14 中国科学院沈阳应用生态研究所 Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device

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Granted publication date: 20160706

Termination date: 20170115