CN202898432U - Device for pressure culture of cells - Google Patents
Device for pressure culture of cells Download PDFInfo
- Publication number
- CN202898432U CN202898432U CN 201220604058 CN201220604058U CN202898432U CN 202898432 U CN202898432 U CN 202898432U CN 201220604058 CN201220604058 CN 201220604058 CN 201220604058 U CN201220604058 U CN 201220604058U CN 202898432 U CN202898432 U CN 202898432U
- Authority
- CN
- China
- Prior art keywords
- pressure
- bottle
- way cock
- culturing
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The utility model relates to a device for pressure culture of cells. The device is used for solving the problems that the cells are easily polluted, the pressure regulation capability is poor, and the like due to the adoption of a centrifuge in the pressure culture of the cells. The device comprises a pressure culture flask, wherein a flask inlet three-way switch is connected to a flask mouth of the pressure culture flask, the other end of the flask inlet three-way switch is connected to one end of a pressure gauge, and the other end of the pressure gauge is connected with a gas inlet pipe. The device for pressure culture of cells has the advantages that the structure is simple, the operation is convenient, the pressure of gas in the culture flask can be accurately controlled for a long time, pressure tests can be continuously carried out, and researches related to pressure cell biology can be carried out conveniently.
Description
Technical field
The utility model relates to a kind of cell in vitro culture apparatus, relates in particular to cell pressure culture apparatus a kind of simple in structure, easy to operate.
Background technology
Osseous tissue is subject to the impact that mechanical environment changes easily, the adjustment of bone amount and keeping depends on the mechanics of machinery that bone itself is subject to a great extent to stimulate, pressure has material impact to scleroblast and chondrocyte's biological behaviour, people are in order to study different pressures to the effect of cell, the way of mainly taking is the centrifugal pressure of whizzer, culturing bottle is put into airtight culture vessel, adopts pressurized air to increase pressure.These methods exist the time length short, the easy contamination of cells of experimental installation, the characteristics such as the pressure regulating power is poor.
Summary of the invention
The problems such as the utility model has mainly solved in the cultivation of cell pressure and adopted whizzer, has easy contamination of cells, and the pressure regulating power is poor provide a kind of simple in structure, easy to operate cell pressure culture apparatus.
The technical scheme that its technical problem that solves the utility model adopts is: a kind of cell pressure culture apparatus, comprise the pressure culturing bottle, bottleneck at the pressure culturing bottle is connected with into a bottle three-way cock, this advances a bottle three-way cock the other end and is connected on the manometric end manometric the other end connection inlet pipe.The utility model is simple in structure, and is easy to operate, can accurately control for a long time gaseous tension in the culturing bottle, continues to carry out pressure test, conveniently carries out the correlative study of pressure cytobiology.The utility model is controlled in addition, and experimental result is repeatable strong.The inlet pipe the other end connects diffuser.
As a kind of preferred version, described inlet pipe is by connecting Bonding pressure table behind the air inlet three-way cock.Bonding pressure machine handled easily personnel know culturing bottle internal pressure situation.The air inlet three-way cock is used for regulating the air inlet of inlet pipe.
As a kind of preferred version, described pressure culturing bottle comprises culturing room and bottleneck chamber, and the bottleneck chamber is communicated with culturing room, and this bottleneck chamber opening one end shrinks and forms bottleneck.This bottleneck structure is convenient to be connected with pipeline.
As a kind of preferred version, be high pressure resistant bottle at described pressure culturing bottle, the pressure culturing bottle is tightly connected by advancing bottle conduit and advancing a bottle three-way cock.The pressure culturing bottle can be high pressure resistant, can better carry out pressure experiment, and this gold medal three-way cock is used for regulating the air inlet of bottle conduit.
The utility model has the advantages that: simple in structure, easy to operate, can accurately control for a long time gaseous tension in the culturing bottle, continue to carry out pressure test, conveniently carry out the correlative study of pressure cytobiology.
Description of drawings
Fig. 1 is a kind of structural representation of the present utility model.
1-air inlet three-way cock 2-tensimeter 3-advances a bottle three-way cock 4-inlet pipe 5-and enters bottle conduit 6-pressure culturing bottle 7-8-of a culturing room bottleneck chamber
Embodiment
Below by embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is further described.
Embodiment:
A kind of cell pressure of present embodiment culture apparatus, as shown in Figure 1, comprise culturing bottle 6, advance bottle three-way cock 3, tensimeter 2 and air inlet three-way cock 1, this culturing bottle comprises culturing room 7 and bottleneck chamber 8 two portions, the bottleneck chamber is connected with culturing room, bottleneck chamber opening one end shrinks and forms bottleneck, and this culturing bottle is high pressure resistant bottle.The bottleneck of culturing bottle is sealably coupled on an end of bottle three-way cock 3 by advancing bottle conduit 5, this advances a bottle three-way cock the other end and is connected on tensimeter one end, the tensimeter the other end is connected on the end of air inlet three-way cock, the other end of air inlet three-way cock connects inlet pipe 4, and this inlet pipe is connected on the diffuser.
This cell pressure culture apparatus is got first rat osteoblast and is put into the pressure culturing bottle in use, opens the air inlet three-way cock and advances a bottle three-way cock.Pass into gas by inlet pipe, tensimeter record pressure change when pressure reaches design requirements, is closed first the air inlet three-way cock, after close into a bottle three-way cock, then remove tensimeter and air inlet three-way cock.Can make the culturing bottle internal pressure continue to reach design requirements cultivates.
Specific embodiment described herein only is to the explanation for example of the present invention's spirit.Those skilled in the art can make various modifications or replenish or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Although this paper has more used air inlet three-way cock, tensimeter, advances a bottle three-way cock, inlet pipe, advance the terms such as bottle conduit, do not get rid of the possibility of using other term.Using these terms only is in order to describe more easily and explain essence of the present invention; They are construed to any additional restriction all is contrary with spirit of the present invention.
Claims (4)
1. cell pressure culture apparatus, it is characterized in that: comprise pressure culturing bottle (6), bottleneck at the pressure culturing bottle is connected with into a bottle three-way cock (3), and this advances on the end that bottle three-way cock the other end is connected to a tensimeter (2), and the manometric the other end connects inlet pipe (4).
2. a kind of cell pressure culture apparatus according to claim 1 is characterized in that described inlet pipe (4) is by connecting the rear Bonding pressure table (2) of air inlet three-way cock (1).
3. a kind of cell pressure culture apparatus according to claim 1 is characterized in that described pressure culturing bottle (6) comprises culturing room (7) and bottleneck chamber (8), and the bottleneck chamber is communicated with culturing room, and this bottleneck chamber opening one end shrinks and forms bottleneck.
4. according to claim 1 and 2 or 3 described a kind of cell pressure culture apparatuses, it is characterized in that at described pressure culturing bottle be high pressure resistant bottle, the pressure culturing bottle is by advancing a bottle conduit (5) and advancing a bottle three-way cock (3) and be tightly connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220604058 CN202898432U (en) | 2012-11-15 | 2012-11-15 | Device for pressure culture of cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220604058 CN202898432U (en) | 2012-11-15 | 2012-11-15 | Device for pressure culture of cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202898432U true CN202898432U (en) | 2013-04-24 |
Family
ID=48119124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220604058 Expired - Lifetime CN202898432U (en) | 2012-11-15 | 2012-11-15 | Device for pressure culture of cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202898432U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132276A (en) * | 2015-08-24 | 2015-12-09 | 郑州点石生物技术有限公司 | Blood culture bottle with positive alarm device |
-
2012
- 2012-11-15 CN CN 201220604058 patent/CN202898432U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132276A (en) * | 2015-08-24 | 2015-12-09 | 郑州点石生物技术有限公司 | Blood culture bottle with positive alarm device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130424 |
|
| CX01 | Expiry of patent term |