CN204188610U - A kind of myocardium vessel microbubble perfusion device - Google Patents
A kind of myocardium vessel microbubble perfusion device Download PDFInfo
- Publication number
- CN204188610U CN204188610U CN201420580635.9U CN201420580635U CN204188610U CN 204188610 U CN204188610 U CN 204188610U CN 201420580635 U CN201420580635 U CN 201420580635U CN 204188610 U CN204188610 U CN 204188610U
- Authority
- CN
- China
- Prior art keywords
- microbubble
- myocardium vessel
- perfusion
- preparation facilities
- myocardium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model relates to laboratory equipment technical field, and the utility model provides a kind of myocardium vessel microbubble perfusion device, comprises myocardium vessel pressure flesh and moves system and two microbubble preparation facilities; Described myocardium vessel pressure flesh moves system and comprises compressed gas source, control desk, perfusion bottle, bath, waste liquid bottle, inverted microscope, video camera, computing machine; Two described microbubble preparation facilities, structure is identical, is all located at myocardium vessel pressure flesh and moves in the bath of system.The utility model utilizes myocardium vessel pressure flesh to move system for myocardium vessel and provides perfusion, the size of the microbubble generated by regulating microbubble preparation facilities and density, realize the accurate control of myocardium vessel section microbubble perfusion intensi-ty, meet the demand of different experiments research.
Description
Technical field
The utility model relates to laboratory equipment technical field, is specifically related to a kind of myocardium vessel microbubble perfusion device for studying decompression sickness bubble to the pathogenic effects of blood vessel endothelium and mechanism.
Background technology
Along with the sustainable development of China's marine economy and day by day highlighting of ocean military status, all kinds of diving activity is day by day frequent.Decompression sickness is the topmost medical problem faced in diving activity, and its pathogenesis is that body interior (comprising Ink vessel transfusing) forms bubble.Further investigation Ink vessel transfusing bubble causes the effect of body injury, illustrates pathogenesis of decompression sickness, explores effective prophylactico-therapeutic measures, becomes the emphasis of submarine medicine and hyperbar medical research.After Ink vessel transfusing bubble is formed, the also endothelial cell injury of contact at first, and then excite a series of immunization inflammatory reaction, advance decompression sickness process.Mostly be to make spontaneous generation bubble in animal body by simulated dive fast decompression about the research of decompression sickness bubble to the pathogenic effect of blood vessel endothelium and mechanism at present.The method can the generation of real simulation diving disease, but there is serious individual difference due to decompression sickness, this mode cannot the generation of fixing quantity bubble, and be difficult to further investigate the pathogenic amount-result relation of bubble, also have impact on the degree of depth of Mechanism Study and the efficiency of the effective prophylactico-therapeutic measures of screening.At present for the microbubble preparation facilities complex structure of other field experimental study, processing difficulties, the microbubble volume difference of generation is comparatively large, is difficult to accurately control microbubble density simultaneously.Ripe myocardium vessel microbubble Perfusion is there is no in decompression sickness research field.
Therefore, if specific micro bubble generator can be designed, the microbubble of target sizes and density can be generated as required, in conjunction with international advanced myocardium vessel Perfusion, build myocardium vessel microbubble perfusion device, get rid of disturbing factor in body complicated in decompression sickness zoopery, analyze the amount-result relation that bubble causes a disease, the mechanism that in the further investigation circulation system, bubble causes a disease and prophylactico-therapeutic measures etc. produce and help greatly.
Utility model content
The purpose of this utility model be for research decompression sickness bubble to the damage mechanisms of blood vessel endothelium, explore a kind of myocardium vessel microbubble perfusion device that effective prophylactico-therapeutic measures provides microbubble perfusion intensi-ty accurately to control.
The technical problems to be solved in the utility model is, suitable source of the gas prepared composition and decompression sickness bubble phase bubble together should be used, for myocardium vessel provides microbubble perfusion, different microbubble perfusion intensi-ty is provided by accurate control microbubble size, density, and then analyzes the dose-effect relationship of bubble damage again.
On July 8th, 2014, the applicant has applied for that number of patent application is CN201420373485.4, denomination of invention is the Chinese utility model patent application of " a kind of microbubble preparation facilities ", the applicant on this basis, in conjunction with myocardium vessel Perfusion, build a kind of myocardium vessel microbubble perfusion device.
Technical solutions of the utility model are as follows:
A kind of myocardium vessel microbubble perfusion device, comprises myocardium vessel pressure flesh and moves system and two microbubble preparation facilities;
Myocardium vessel pressure flesh moves system, comprises compressed gas source, perfusion system, bath;
Two microbubble preparation facilities, structure is identical, is all located at myocardium vessel pressure flesh and moves in the bath of system, comprise housing, cap, draft tube, feed tube, air inlet glass capillary, reducing glass capillary and effuser; Described housing clear hollow, one end of housing connects effuser, and the other end of housing seals with cap; Described draft tube is communicated with housing by cap, and connects air inlet glass capillary, reducing glass capillary and effuser successively; Described feed tube enters housing by cap; The centre of described reducing glass capillary is narrow section, and the head end of described air inlet glass capillary stretches into the narrow section of reducing glass capillary;
Described microbubble preparation facilities, is connected with feeder by draft tube;
The perfusion system that one of them microbubble preparation facilities moves system by feed tube, effuser with myocardium vessel pressure flesh is connected.
Preferably, described microbubble preparation facilities, is connected with feeder by draft tube, and described feeder is compressed nitrogen device.
Preferably, another microbubble preparation facilities is connected with infusion pump by feed tube, is connected with waste liquid cylinder by effuser.
Preferably, one of them microbubble preparation facilities connects by feed tube the perfusion bottle that myocardium vessel pressure flesh moves the perfusion system of system; The waste liquid bottle that myocardium vessel pressure flesh moves the perfusion system of system is connected by effuser.
Preferably, described microbubble preparation facilities prepares the microbubble of diameter between 150 ~ 500 μm.
Preferably, described myocardium vessel pressure flesh moves system and also comprises inverted microscope, video camera, computing machine.
Preferably, it is DMT company of Denmark PressureMyograph 120cp that described myocardium vessel pressure flesh moves system, comprises compressed gas source, control desk, perfusion bottle, bath, waste liquid bottle, inverted microscope, video camera, computing machine.
Preferably, described myocardium vessel pressure flesh moves system and observes vessel segment change by inverted microscope, and with video camera real time record, is sent to computing machine and carries out automatic analysis, and then obtains vessel segment indices parameter.
Because the bath of DMT company of Denmark Pressure Myograph 120cp exists many places interface with the pipeline between liquid perfusion bottle, especially bath inlet three-way pipe place, bubble is easily caused to gather fusion, in order to reduce unnecessary interface, prioritizing selection is at bath inside access microbubble preparation facilities.
Preferably, described myocardium vessel pressure flesh moves system, and compressed gas source is 95%O
2, 5%CO
2, the O needed for vessel segment survival was both provided
2and pH, system perfusion power is provided again.
Preferably, described microbubble preparation facilities, by regulating correlation parameter, can prepare the microbubble of diameter between 150 ~ 500 μm.Under the constant prerequisite of Air Bubble Size, stable formation speed can be maintained, under the constant prerequisite of formation speed, also can maintain stable Air Bubble Size, the microbubble perfusion intensi-ty of density, size, Hemoperfusion time three parameter various combinations can be provided thus.
The preferred version of microbubble preparation facilities, can with further reference to the CN201420373485.4 of the applicant's earlier application, a kind of microbubble preparation facilities.Such as, in a preferred version of the present utility model, described draft tube, air inlet glass capillary, reducing glass capillary and effuser are positioned on the axis of housing.Preferably, the head end drawing-down of described air inlet glass capillary, internal diameter is 15 ~ 100 μm.Preferably, the head end end of described air inlet glass capillary is wedge shape, and surface slope is 45 °.Preferably, the internal diameter of the narrow section of described reducing capillary glass is 30 ~ 500 μm.Preferably, the external diameter of described air inlet glass capillary is 1 ~ 1.5mm, internal diameter is 0.75 ~ 0.9mm.Preferably, described draft tube internal diameter is 2 ~ 3mm, feed tube internal diameter is 2 ~ 3mm.Preferably, the internal diameter of described effuser is 1 ~ 2mm.
The beneficial effects of the utility model are, microbubble preparation facilities uses nontoxic material to make, and can ensure that utility model itself is harmless to experimental subjects; Use transparent material is made, direct observational record microbubble under being convenient to microscope; In conjunction with myocardium vessel research advanced technology means, realize the external microbubble perfusion of vessel segment; Accurately can control microbubble size, generation speed, realize varying strength microbubble perfusion; Structure is simple, easy to process, with low cost, there is not blockage problem, reusable.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the enlarged drawing of associated components in bath in the utility model;
Fig. 3 is the structural representation of microbubble preparation facilities of the present utility model;
Fig. 4 is the enlarged drawing of two glass capillaries in microbubble preparation facilities of the present utility model;
Icon implication is:
1,1 '-microbubble preparation facilities;
11-draft tube; 12-housing; 13-effuser;
14-feed tube; 15-air inlet glass capillary;
16-reducing glass capillary; 17-cap;
2-infusion pump; 3-compressed nitrogen device;
4-inverted microscope; 5-video camera; 6-myocardium vessel;
7-waste liquid cylinder; 8-compressed oxygen device; 9-perfusion bottle;
10-waste liquid bottle; 20-control desk; 30-computing machine;
40-microbubble; 50-bath.
Embodiment
According to embodiment and accompanying drawing thereof, preferred forms of the present utility model is described in further detail, but enforcement of the present utility model is also not only in this.
Embodiment 1:
As depicted in figs. 1 and 2, in bath 50, microbubble preparation facilities 1 and microbubble preparation facilities 1 ' is accessed.
Microbubble preparation facilities 1 utilizes myocardium vessel pressure flesh to move the perfusion system of system own and provides liquid perfusion, compressed oxygen device 8 Access Control platform 20, for perfusion system provides pressure, and provides required oxygen and pH for myocardium vessel 6.Control desk 20 is provided with perfusion bottle 9 and waste liquid bottle 10.
The gas of compressed nitrogen device 3, after two-stage decompression, is connected to microbubble preparation facilities 1 through flexible pipe.On this section of flexible pipe, connect another microbubble preparation facilities 1 ' with three-way pipe.This gas is useful prepares microbubble.The feed tube of microbubble preparation facilities 1 ' is connected to infusion pump 2, to provide perfusion liquid.
Before two microbubble preparation facilities input gases, a whole set of pipeline first fills liquid.Connect myocardium vessel 6, after activation recovering, regulating transfusion pump 2, makes flow rate of liquid in two microbubble preparation facilities consistent.By three-way pipe, pressure gas is introduced microbubble preparation facilities 1 ', adjustments of gas input pressure produces microbubble 40, collects with waste liquid cylinder 7.Rotate three-way pipe and gas is proceeded to microbubble preparation facilities 1, size in same microbubble preparation facilities 1 ', microbubble 40 that quantity is consistent in microbubble preparation facilities 1, can be produced.
Give the microbubble perfusion of myocardium vessel 6 certain hour, move inverted microscope 4 in system and video camera 5 Real Time Observation by myocardium vessel pressure flesh and record the reaction of Air Bubble Size, quantity and myocardium vessel, being transferred to computing machine, for subsequent analysis.
Above-mentioned microbubble preparation facilities 1,1 ', structure is identical, as shown in Figure 3 and Figure 4, comprises housing 12, cap 17, draft tube 11, feed tube 14, air inlet glass capillary 15, reducing glass capillary 16 and effuser 13; Described housing 12 clear hollow, one end of housing connects effuser 13, and the other end of housing seals with cap 17; Described draft tube 11 is communicated with housing by cap, and connects air inlet glass capillary 15, reducing glass capillary 16 and effuser 13 successively; Described feed tube 14 enters housing by cap; The centre of described reducing glass capillary 16 is narrow section, and the head end of described air inlet glass capillary 15 stretches into the narrow section of reducing glass capillary 16.
Below the preferred embodiment that the utility model is created is illustrated, but the utility model is created and is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modification or replacement under the prerequisite without prejudice to the utility model creative spirit, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (7)
1. a myocardium vessel microbubble perfusion device, is characterized in that, this myocardium vessel microbubble perfusion device comprises myocardium vessel pressure flesh and moves system and two microbubble preparation facilities;
Myocardium vessel pressure flesh moves system, comprises compressed gas source, perfusion system, bath;
Two microbubble preparation facilities, structure is identical, is all located at myocardium vessel pressure flesh and moves in the bath of system, comprise housing, cap, draft tube, feed tube, air inlet glass capillary, reducing glass capillary and effuser; Described housing clear hollow, one end of housing connects effuser, and the other end of housing seals with cap; Described draft tube is communicated with housing by cap, and connects air inlet glass capillary, reducing glass capillary and effuser successively; Described feed tube enters housing by cap; The centre of described reducing glass capillary is narrow section, and the head end of described air inlet glass capillary stretches into the narrow section of reducing glass capillary;
Described microbubble preparation facilities, is connected with feeder by draft tube;
The perfusion system that one of them microbubble preparation facilities moves system by feed tube, effuser with myocardium vessel pressure flesh is connected.
2. a kind of myocardium vessel microbubble perfusion device according to claim 1, it is characterized in that, described microbubble preparation facilities, is connected with feeder by draft tube, and described feeder is compressed nitrogen device.
3. a kind of myocardium vessel microbubble perfusion device according to claim 1, it is characterized in that, another microbubble preparation facilities is connected with infusion pump by feed tube, is connected with waste liquid cylinder by effuser.
4. a kind of myocardium vessel microbubble perfusion device according to claim 1, is characterized in that, one of them microbubble preparation facilities connects by feed tube the perfusion bottle that myocardium vessel pressure flesh moves the perfusion system of system; The waste liquid bottle that myocardium vessel pressure flesh moves the perfusion system of system is connected by effuser.
5. a kind of myocardium vessel microbubble perfusion device according to claim 1, it is characterized in that, described microbubble preparation facilities prepares the microbubble of diameter between 150 ~ 500 μm.
6. a kind of myocardium vessel microbubble perfusion device according to claim 1, is characterized in that, described myocardium vessel pressure flesh moves system and also comprises inverted microscope, video camera, computing machine.
7. a kind of myocardium vessel microbubble perfusion device according to claim 1, is characterized in that, described myocardium vessel pressure flesh moves the PressureMyograph 120cp that system is DMT company of Denmark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420580635.9U CN204188610U (en) | 2014-10-09 | 2014-10-09 | A kind of myocardium vessel microbubble perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420580635.9U CN204188610U (en) | 2014-10-09 | 2014-10-09 | A kind of myocardium vessel microbubble perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204188610U true CN204188610U (en) | 2015-03-04 |
Family
ID=52620593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420580635.9U Expired - Fee Related CN204188610U (en) | 2014-10-09 | 2014-10-09 | A kind of myocardium vessel microbubble perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204188610U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109192033A (en) * | 2018-10-12 | 2019-01-11 | 中国人民解放军海军军医大学海军医学研究所 | A kind of human decompression's disease simulation model and its construction method |
| CN109240361A (en) * | 2018-09-10 | 2019-01-18 | 江西省医学科学院 | A kind of the arterial perfusion pressure control device and compress control method of myocardium vessel |
-
2014
- 2014-10-09 CN CN201420580635.9U patent/CN204188610U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109240361A (en) * | 2018-09-10 | 2019-01-18 | 江西省医学科学院 | A kind of the arterial perfusion pressure control device and compress control method of myocardium vessel |
| CN109240361B (en) * | 2018-09-10 | 2023-11-07 | 江西省医学科学院 | Arterial perfusion pressure control device and pressure control method for isolated blood vessel |
| CN109192033A (en) * | 2018-10-12 | 2019-01-11 | 中国人民解放军海军军医大学海军医学研究所 | A kind of human decompression's disease simulation model and its construction method |
| CN109192033B (en) * | 2018-10-12 | 2021-10-22 | 中国人民解放军海军军医大学海军医学研究所 | Human body decompression sickness simulation model and construction method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bonvillain et al. | Nonhuman primate lung decellularization and recellularization using a specialized large-organ bioreactor | |
| CN103805511B (en) | The arteries simulation micro fluidic device directly can observed under high power objective | |
| CN204188610U (en) | A kind of myocardium vessel microbubble perfusion device | |
| CN102329729A (en) | Culture system for simulating microgravity effect of suspension cells | |
| CN102676446A (en) | Method and device for loading cell fluid stress on deformable curved surface and experimental platform | |
| CN108641931A (en) | A kind of digitlization microarray organ chip and its application | |
| CN108893248A (en) | Piezoelectric supersonic microinjector | |
| Guler et al. | Sperm selection and embryo development: a comparison of the density gradient centrifugation and microfluidic chip sperm preparation methods in patients with astheno-teratozoospermia | |
| CN105385597A (en) | Cell culture bag and application thereof | |
| CN105181295B (en) | A kind of method and apparatus for controlling and studying bubbling polymerization process | |
| Ginga et al. | Perfusion system for modification of luminal contents of human intestinal organoids and realtime imaging analysis of microbial populations | |
| CN212688016U (en) | Biomembrane dynamic culture system under simulation in vivo flow environment | |
| CN205420436U (en) | Cell culturing bag | |
| CN209098693U (en) | A kind of intermittent streaming electrotransfection device | |
| CN203882515U (en) | Carbon dioxide preparation and property experiment device | |
| CN208426261U (en) | A kind of automatic filling system fixed suitable for rats and mice intracerebral | |
| CN107536615A (en) | A kind of hemospast | |
| CN204229728U (en) | A kind of chlorella photosynthesis teaching and experimental demonstration device | |
| CN201122375Y (en) | Photosynthesis reaction device | |
| CN209243073U (en) | A kind of intermittent streaming electrotransfection device | |
| CN212247072U (en) | Micro-fluidic device for in-vitro 3D cell and tissue culture | |
| Li et al. | Robotic intracellular pressure measurement using micropipette electrode | |
| CN104700700A (en) | Experimental box for studying influence on photosynthesis caused by external environment | |
| CN206089707U (en) | Ginseng cell culture device | |
| CN202615666U (en) | Demonstration device for permeation action |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20191009 |