CN208426261U - A kind of automatic filling system fixed suitable for rats and mice intracerebral - Google Patents
A kind of automatic filling system fixed suitable for rats and mice intracerebral Download PDFInfo
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- CN208426261U CN208426261U CN201721718571.4U CN201721718571U CN208426261U CN 208426261 U CN208426261 U CN 208426261U CN 201721718571 U CN201721718571 U CN 201721718571U CN 208426261 U CN208426261 U CN 208426261U
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Abstract
The utility model provides a kind of automatic filling system fixed suitable for rats and mice intracerebral.The soda liquor container, the one end for fixing liquid container one, fixed liquid container two and input duct are interconnected by four-way valve, the other end of input duct is arranged in transparent confined chamber, perfusion needle is connected with the other end of input duct, peristaltic pump and filter are serially connected in input duct, rats and mice fixed plate is arranged in transparent confined chamber, rats and mice fixed plate is equipped with rats and mice fixing clamp, the upper end of waste recycling pipeline is connected with the bottom of transparent confined chamber, and the lower end of waste recycling pipeline is connected with devil liquor recovery container.The utility model uses automatic filling system, the rate adaptation of fixed different phase, it is ensured that the stability and controllability of experimental result.Transparent confined chamber can contact of the isolation experiment personnel with harmful liquid, be conducive to personnel health and environmental protection.Waste collecting device then guarantees that the safe and reasonable of experimental waste liquid is collected, and improves working efficiency and safety.
Description
Technical field
The utility model relates to a kind of automatic filling systems fixed suitable for rats and mice intracerebral, belong to automatic filling system
Technical field.
Background technique
Brain tissue Internal fixation technology is to be recycled in zoopery using animal blood, by heart cathetrization, by formaldehyde or penta
Dialdehyde push-in is internal, to achieve the purpose that brain tissue is fixed.The effectively fixation of brain tissue short time be observation brain tissue cell form,
Ultra microstructure and the important prerequisite for carrying out the correlative studys such as immunochemistry dyeing, the success or failure of interior fixation are directly related to experimental result
Authenticity and reliability.There are following several drawbacks for method for filling at present:
1, push-in rate is uncontrollable.Conventional method uses syringe, needs experimenter to be manually pushed back into, is unable to control each note
The speed of the uniform dynamics and push-in that enter.It can guarantee the stability of push-in dynamics using Constant pressure perfusion, but its push-in speed is
It is constant, and the basic operation of interior fixation its process is first quick and back slow, i.e., to need quickly push-in PBS cleaning solution at perfusion initial stage,
Then quickly it is pushed into fixer, it is therefore an objective to guarantee that the completion when severe depletion of oxygen does not occur of tissue or cell is fixed.Then again with
One constant rate of speed is slowly pushed into the exchange equilibrium for reaching tissue and perfusion liquid.It is excessively slow to be pushed into speed, causes not having in the short time
Effect is fixed, and brain tissue non-specific lesion occurs because fixing not in time.Be pushed into rate it is too fast, cause big quantity of fluid outflow or
Flow into body circulation.The rate adaptation of interior fixed different phase, for avoiding the Cell infusion of the high oxygen consumption organ as brain tissue
Damage it is particularly important, it is existing to be manually pushed back into or Constant pressure perfusion is then unable to satisfy the controllability of perfusion rate, influence experiment knot
The reliability of fruit.
2, experimenter's inherently safe hidden danger.Perfusion liquid is mostly formaldehyde or glutaraldehyde, and aldehydes ingredient effumability is exposed to
In air, the health of scientific research personnel can be seriously endangered during the experiment, while in filling process, it is blind due to perfusion
Mesh can cause a large amount of reagent waste, increase experimental cost and be unfavorable for environmental protection.
3, perfusion, heavy workload cannot achieve automatic filling manually.Traditional method for filling is often two artificial works, from
Perfusion starts to terminating, and experimenter needs manual whole-process control, cannot achieve liberation both hands, increases experiment manpower expenditure.
4, waste liquid not easily collecting and processing.During the experiment, many waste liquids can be generated, can generally be incited somebody to action after experimenter's experiment
Waste liquid pours into waste liquid cylinder, and such as one animal of every completion, which is toppled over, once will affect the efficiency of experiment, topple over again after being such as fully completed and
It is exposed to waste liquid in air for a long time, increases the risk of environmental pollution and experimenter's safety.
Authorization Notice No. is CN203647530U, authorized announcement date is on 06 18th, 2014, a kind of entitled " brain tissue
Interior fixed constant pressure perfusion apparatus " utility model patent, effect is to guarantee pressure balance by the mercury scale in U-tube.
Keep pressure in 120mmHg level simultaneously.To guarantee the pressure balance in inspection pressure pipe and intrusion pipe, the organ of measured object brain tissue
Position should keep same level with 0 scale of pressure gauge.Although the utility model allows perfusion to protect by the mercury scale in U-tube
It holds in a relative constant pressure limit, but due to being still irrigated with syringe, by manually controlling, pressure is not steady enough
It is fixed.To tested animal perfusion formalin (formaldehyde) or glutaraldehyde, since tested animal is not in closed space, waste liquid is sudden and violent
Dew is in air, all harmful to testing crew and environment.
Utility model content
Purpose of the utility model is to solve the above-mentioned problems of the prior art, and then provide a kind of be suitable for greatly
Fixed automatic filling system in mouse brain.
The purpose of this utility model is achieved through the following technical solutions:
A kind of automatic filling system fixed suitable for rats and mice intracerebral, comprising: soda liquor container, fixed liquid container one,
Fixed liquid container two, four-way valve, input duct, peristaltic pump, rats and mice fixed plate, transparent confined chamber, waste recycling pipeline, waste liquid
Returnable, rats and mice fixing clamp, perfusion needle and filter, soda liquor container, fixed liquid container one, fixed liquid container two and into
The one end in liquid pipe road is interconnected by four-way valve, and the other end of input duct is arranged in transparent confined chamber, perfusion needle and feed liquor
The other end of pipeline is connected, and peristaltic pump and filter are serially connected in input duct, and rats and mice fixed plate is arranged transparent closed
Interior, rats and mice fixed plate are equipped with rats and mice fixing clamp, and the upper end of waste recycling pipeline is connected with the bottom of transparent confined chamber
Logical, the lower end of waste recycling pipeline is connected with devil liquor recovery container.
The utility model can effectively avoid four kinds described in background technique and happen: be filled using automatic filling system
Note, the rate adaptation of fixed different phase, it is ensured that the stability and controllability of experimental result.Reality can be isolated in transparent confined chamber
Contact of the personnel with harmful liquid is tested, personnel health and environmental protection are conducive to.Waste collecting device then guarantees experimental waste liquid
Safe and reasonable is collected, and working efficiency and safety are improved.In addition, the present apparatus can liberate both hands to the greatest extent, convenient for testing
Observation and guidance in journey.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that the utility model is suitable for the fixed automatic filling system of rats and mice intracerebral.
Appended drawing reference in figure, 1 is soda liquor container, and 2 be fixed liquid container one, and 3 be fixed liquid container two, and 4 be four-way
Valve, 5 be input duct, and 6 be peristaltic pump, and 7 be rats and mice fixed plate, and 8 be transparent confined chamber, and 9 be waste recycling pipeline, and 10 be useless
Liquid returnable, 11 be rats and mice fixing clamp, and 12 be perfusion needle, and 13 be filter.
Specific embodiment
Be described in further detail below in conjunction with attached drawing to the utility model: the present embodiment is with the utility model skill
Implemented under premised on art scheme, give detailed embodiment, but the protection scope of the utility model be not limited to it is following
Embodiment.
As shown in Figure 1, a kind of automatic filling system fixed suitable for rats and mice intracerebral, packet involved in the present embodiment
Include: soda liquor container 1, fixed liquid container 1, fixed liquid container 23, four-way valve 4, input duct 5, peristaltic pump 6, rats and mice are solid
Fixed board 7, transparent confined chamber 8, waste recycling pipeline 9, devil liquor recovery container 10, rats and mice fixing clamp 11, perfusion needle 12 and filtering
Device 13, soda liquor container 1, fixed liquid container 1, fixed liquid container 23 are mutually interconnected with one end of input duct 5 by four-way valve 4
Logical, the other end of input duct 5 is arranged in transparent confined chamber 8, and perfusion needle 12 is connected with the other end of input duct 5, into
Peristaltic pump 6 and filter 13 are serially connected on liquid pipe road 5, rats and mice fixed plate 7 is arranged in transparent confined chamber 8, and rats and mice is fixed
Plate 7 is equipped with rats and mice fixing clamp 11, and the upper end of waste recycling pipeline 9 is connected with the bottom of transparent confined chamber 8, devil liquor recovery
The lower end of pipeline 9 is connected with devil liquor recovery container 10.
The front end of the perfusion needle 12 is smooth circular structure.
The rats and mice fixing clamp 11 is five.Respectively correspond four limbs and the head of rats and mice.
Before implementing perfusion, required cleaning solution (such as physiological saline, PBS buffer solution will be previously charged into soda liquor container 1
Deng), fixer (formaldehyde or glutaraldehyde etc.) will be packed into fixed liquid container 1 and fixed liquid container 23, after connecting, open
The upper cover of transparent confined chamber 8, rat or mouse are fixed in rats and mice fixed plate 7, and perfusion needle 12 is inserted into rat or mouse
Ventricle.The upper cover of transparent confined chamber 8 is closed, peristaltic pump 6 is adjusted, sets flow velocity and time, start to be perfused, by adjusting four
Port valve 4 can control the injection between different perfusion liquids.In filling process, generated waste liquid can pass through waste recycling pipeline
9 automatically flow into devil liquor recovery container 10, complete waste collection.Practical application shows that design is fully achieved in the utility model
It is required that.
The preferable specific embodiment of the above, only the utility model, these specific embodiments are all based on this
Different implementations under utility model general idea, and the protection scope of the utility model is not limited thereto, it is any ripe
Know those skilled in the art within the technical scope disclosed by the utility model, any changes or substitutions that can be easily thought of, all
It should cover and be within the protection scope of the utility model.Therefore, the protection scope of the utility model should be with claims
Subject to protection scope.
Claims (3)
1. a kind of automatic filling system fixed suitable for rats and mice intracerebral, comprising: soda liquor container (1), fixed liquid container one
(2), fixed liquid container two (3), four-way valve (4), input duct (5), peristaltic pump (6), rats and mice fixed plate (7), transparent closed
Room (8), waste recycling pipeline (9), devil liquor recovery container (10), rats and mice fixing clamp (11), perfusion needle (12) and filter
(13), which is characterized in that soda liquor container (1) fixes liquid container one (2), fixed liquid container two (3) and input duct (5)
One end is interconnected by four-way valve (4), the setting of the other end of input duct (5) in transparent confined chamber (8), perfusion needle (12) with
The other end of input duct (5) is connected, and peristaltic pump (6) and filter (13) is serially connected in input duct (5), rats and mice is fixed
Plate (7) setting is in transparent confined chamber (8), and rats and mice fixed plate (7) is equipped with rats and mice fixing clamp (11), waste recycling pipeline
(9) upper end is connected with the bottom of transparent confined chamber (8), the lower end and devil liquor recovery container (10) of waste recycling pipeline (9)
It is connected.
2. the automatic filling system fixed suitable for rats and mice intracerebral according to claim 1, which is characterized in that the filling
The front end for flowing needle (12) is smooth circular structure.
3. the automatic filling system fixed suitable for rats and mice intracerebral according to claim 1, which is characterized in that described big
Mouse fixing clamp (11) is five.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721718571.4U CN208426261U (en) | 2017-12-11 | 2017-12-11 | A kind of automatic filling system fixed suitable for rats and mice intracerebral |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721718571.4U CN208426261U (en) | 2017-12-11 | 2017-12-11 | A kind of automatic filling system fixed suitable for rats and mice intracerebral |
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| Publication Number | Publication Date |
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| CN208426261U true CN208426261U (en) | 2019-01-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201721718571.4U Active CN208426261U (en) | 2017-12-11 | 2017-12-11 | A kind of automatic filling system fixed suitable for rats and mice intracerebral |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107961095A (en) * | 2017-12-11 | 2018-04-27 | 齐齐哈尔医学院 | A kind of automatic filling system fixed suitable for big mouse intracerebral |
| CN111700062A (en) * | 2020-06-19 | 2020-09-25 | 山东中医药大学 | Intelligent rodent heart perfusion device |
-
2017
- 2017-12-11 CN CN201721718571.4U patent/CN208426261U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107961095A (en) * | 2017-12-11 | 2018-04-27 | 齐齐哈尔医学院 | A kind of automatic filling system fixed suitable for big mouse intracerebral |
| CN111700062A (en) * | 2020-06-19 | 2020-09-25 | 山东中医药大学 | Intelligent rodent heart perfusion device |
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