CN213099890U - Normal-temperature mechanical preservation and re-irrigation system for rat kidney - Google Patents
Normal-temperature mechanical preservation and re-irrigation system for rat kidney Download PDFInfo
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- CN213099890U CN213099890U CN202020639775.4U CN202020639775U CN213099890U CN 213099890 U CN213099890 U CN 213099890U CN 202020639775 U CN202020639775 U CN 202020639775U CN 213099890 U CN213099890 U CN 213099890U
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Abstract
The utility model discloses a normal temperature mechanical preservation and re-irrigation system for rat kidney, which comprises a preservation re-irrigation device, an oxygen supply device, a control device, a micro-injection pump and a constant temperature water bath kettle; the preservation and refilling device is a circulating pipeline device which is formed by connecting an organ storage pool, a peristaltic pump, an oxygenator, a dropping funnel and a pressure receptor in sequence through sterile pipelines, and the organ storage pool, the oxygenator, the dropping funnel, the pressure receptor and the sterile pipelines between the oxygenator and the organ storage pool are all positioned in the constant-temperature water bath kettle; the outlet end of the oxygen supply device is connected into the oxygenator; the control device is connected with the baroreceptor. The middle organ storage pool of the utility model reduces the redundant pipeline connection and the pre-charging volume, saves the space and ensures the kidney temperature and the humidity; the heparin cap has good tightness, the three-way pipe does not need to be opened or closed at any time, and the influence on the system stability when the oxygenated arterial blood is extracted is reduced; the Y-shaped tube and the flow regulator in the oxygen supply device finely regulate the oxygenation quantity of the perfusion liquid.
Description
Technical Field
The utility model belongs to separation kidney fills the field, concretely relates to rat kidney normal atmospheric temperature machinery is preserved and system of irritating again.
Background
Kidney transplantation is an effective means for treating end-stage renal disease, and clinically, in order to make up for the shortage of standard donors (SCD), the use of peripheral donors (ECD) is increased, and Ischemia Reperfusion Injury (IRI) is more serious, which significantly affects the near-term and far-term efficacy of kidney transplantation. At present, the international method for kidney preservation is Static Cold Storage (SCS) at low temperature of about 4 ℃. When SCS is applied to ECD kidneys, the probability of delayed recovery of renal function (DGF) and the occurrence of acute rejection increases after surgery. In view of these limitations, many research groups developed Normothermic Mechanical Perfusion (NMP) for in vitro preservation of donor kidneys. NMP is a technique for in vitro preservation and repair of kidneys using extracorporeal membrane oxygenation at near body temperature.
The existing NMP system mostly takes large animals as research objects, rats are common experimental objects in clinical experiments, but the current perfusion method suitable for the kidneys of large animals is not suitable for the kidneys of rats. In addition, in prior art, because rat kidney is small, have the difficulty in the aspect of intubate and pressure control, rat kidney normal atmospheric temperature machinery that is used for scientific research is preserved and is irritated the system again and do not have unified standard, and the most is independently set up for simulating big animal system principle, has following problem: the renal artery caliber of the rat is fine, the cannula is connected by a scalp needle, the difference between the hose of a scalp needle connector and the caliber of a perfusion pipeline is large, and the accuracy and the stability of monitoring perfusion pressure are influenced; a positive urine collection device; the vein is not intubated, the vein patency is reduced along with the prolonging of the perfusion time, the pressure of the renal vein is gradually increased, and the overall perfusion time and perfusion quality are influenced; in the past, a circulation pipeline is easy to ignore, and a few designs are insulation sleeves which are covered on the circulation pipeline to slow down heat dissipation, but along with the prolonging of perfusion time, the temperature of blood in the pipeline is possibly different from the temperature of blood in a venous pool which is directly heated by a heater, so that red blood cells in perfusate are damaged, and the organ cannot be gradually rewarming; the rat blood is less, the amount of the perfusion fluid prepared by utilizing the rat blood is limited, and a bubble and solid impurity filtering device in large animal perfusion equipment is still used, although the size is reduced, the pre-charging amount of the pipeline perfusion fluid is increased, and the defect of high economic cost is also caused.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a rat kidney normal atmospheric temperature machinery is preserved and is irritated system again, its simple structure, easy and simple to handle, equipment are general, can carry out the external simulation after improving rat kidney preservation quality, extension save time and fine setting and irritate again.
The utility model adopts the following technical scheme:
a normal-temperature mechanical preservation and re-irrigation system for rat kidneys comprises a preservation and re-irrigation device, an oxygen supply device, a control device, a micro-injection pump 1 and a constant-temperature water bath 17; the preservation and refilling device is a circulating pipeline device which is formed by connecting an organ storage pool 2, a peristaltic pump 5, an oxygenator 10, a dropping funnel 15 and a pressure receptor 6 sequentially through a sterile pipeline 7, the outlet end of the organ storage pool 2 is connected with the inlet end of the peristaltic pump 5 through the sterile pipeline 7, the outlet end of the peristaltic pump 5 is connected with the inlet end of the oxygenator 10 through the sterile pipeline 7, a three-way pipe A is arranged on the sterile pipeline 7 between the oxygenator 10 and the dropping funnel 15, the inlet end of the three-way pipe A is connected with the outlet end of the oxygenator 10, one outlet end of the three-way pipe A is connected with the inlet end of the dropping funnel 15, the other outlet end of the three-way pipe A is provided with a heparin cap 14, a three-way pipe B is arranged on the sterile pipeline 7 between the dropping funnel 15 and the pressure receptor 6, the inlet end of the three-way pipe B, the other outlet end is connected with the micro-injection pump 1 through a sterile pipeline 7, the outlet end of the pressure sensor 6 is connected with the inlet end of the organ storage pool 2, wherein the organ storage pool 2, the oxygenator 10, the dropping funnel 15, the pressure sensor 6 and the sterile pipeline 7 from the oxygenator 10 to the organ storage pool 2 are all positioned in a constant temperature water bath 17; the outlet end of the oxygen supply device is connected into the oxygenator 10; the control device is connected with the signal transmission end of the baroreceptor 6.
The oxygen supply device is a pipeline device which is composed of an oxygen bottle 13, a Y-shaped pipe 12 and a flow regulator 11 sequentially through an oxygen pipeline 19, the outlet end of the oxygen bottle 13 is connected with the inlet end of the Y-shaped pipe 12, the outlet end of the Y-shaped pipe 12 is connected with the inlet end of the flow regulator 11, and the outlet end of the flow regulator 11 is connected into the oxygenator 10. The oxygen content in the oxygenator 10 is adjusted by means of the Y-tube 12 and the flow regulator 11 according to the content of the perfusate.
The control device comprises a pressure detection system 9 and a computer detector 8 connected with the pressure detection system, wherein the pressure detection system 9 is connected with the signal transmission end of the baroreceptor 6 to monitor the renal artery pressure in real time.
The organ storage pool 2 is a closed cavity, a top cover plate of the organ storage pool is provided with a round hole M and a urethral orifice 21, the organ storage pool 2 is transversely provided with a thin-layer perforated plate 22 which divides the organ storage pool 2 into an upper cavity and a lower cavity, the upper cavity is an organ chamber 24, the lower cavity is a vein pool 25, the thin-layer perforated plate 22 is provided with a plurality of perforated holes, a round hole N with a corresponding aperture is arranged on the thin-layer perforated plate 22 at a position corresponding to the round hole M of the top cover plate, the bottom of the vein pool 25 is of a V-shaped structure with a high side wall and a low center, and the side wall of the organ chamber 24 is provided with an inlet 26 of; the round hole M is an outlet of the organ storage pool 2, the ureter casing 3 is externally connected with the urethral orifice 21, and the tail end of the ureter casing 3 is provided with a urine collecting pipe 4. The organ chamber 24 is used for placing rat kidneys and the venous pool 25 is used for storing venous blood. The V-shaped structure design at the bottom of the venous pool 25 can lead the blood to be gathered at the V-shaped lowest point, and avoid the red blood cell deposition in the perfusate at other places caused by the local suction of the perfusate by the pipeline. When the device is used, one end of the sterile pipeline 7 extends into the lower venous pool 25 through the round hole M on the top cover plate and the round hole N on the thin-layer pore-leaking plate 22, the other end of the sterile pipeline is connected with the peristaltic pump 5, and the flow is monitored in real time through the peristaltic pump 5. The outlet end of the baroreceptor 6 is connected to an inlet 26 via a sterile tube 7 and into an organ chamber 24 further connected to the renal artery. A urine collection tube 4 at the end of the ureteral cannula 3 is used to collect urine.
The urine collecting pipe 4 is detachably adhered to the outer side wall of the constant-temperature water bath 17. The tape fixing or other sticking methods can be adopted. The urine collection tube may be a sterile EP tube.
The inlet end of the drip chamber 15 is higher than the outlet end thereof. To ensure that arterial blood is bubble free.
The sterile pipeline 7 is a disposable medical rubber hose.
Furthermore, the organ storage pool is a cubic quartz vessel which can be reused after being sterilized at high temperature and high pressure.
The working principle is as follows: before use, the fume hood is sterilized in advance, and the organ storage tank is autoclaved at the same time. When in use, a rat kidney normal-temperature mechanical preservation and re-perfusion system is connected in a fume hood by adopting a sterile pipeline 7 according to the connection mode, a rat kidney is placed in an organ bin 24, prepared venous blood is injected along a round hole M on a top cover plate by using a small-range disposable sterile injector, flows through a leak hole on a thin-layer leak hole plate 22 and enters a venous pool 25, the tail end of the sterile pipeline 7 connected with the inlet end of a peristaltic pump 5 passes through the round hole M and extends into venous blood in the venous pool 25 through a round hole N on the thin-layer leak hole plate 22, the peristaltic pump is started, a constant-temperature water bath kettle is opened to set the temperature to 36-37 ℃, blood re-warming and oxygenation are carried out on the venous pool 24 and a circulation pipeline partially positioned in the constant-temperature water bath kettle for 20min, then a rat renal artery is punctured by using a pressure needle, the renal artery indwelling needle is emptied and then is connected with the sterile pipeline 7 connected with an inlet 26 by a pressure indwelling sensor 6, opening the three-way pipe B, starting the micro-injection pump 1, administering protective medicine to the returned arterial blood, rotating the knob of the peristaltic pump 5, gradually adjusting the flow, and monitoring the pressure through a computer to stabilize the pressure at 65-75 mmHg. And (3) ending the storage stage, taking down the kidney, placing the kidney in ice to flush out residual blood in the kidney, cleaning a circulation passage by using sterile physiological saline, injecting prepared venous blood for repeated perfusion into the venous pool along the circular hole M on the top cover plate by using a small-range disposable sterile injector, closing the three-way pipe B, extending the tail end of the sterile pipeline 7 connected with the inlet end of the peristaltic pump 5 into the venous blood in the venous pool 25 through the circular hole M and the circular hole N on the thin-layer perforated plate 22, starting the peristaltic pump, oxygenating for 20min, connecting the kidney, and controlling the pressure to be 75-85 mmHg.
Compared with the prior art, the utility model has the advantages of it is following and beneficial effect:
(1) except that used repeatedly after the organ storage pool passes through high temperature and high pressure sterilization, this system, part consumptive material is disposable aseptic consumptive material, greatly reduces the pollution probability, connects portably, conveniently dismantles change and transportation.
(2) Partial circulation pipeline, vein pond all submerge in constant temperature water bath, have guaranteed that pipeline and vein pond are synchronous rewarming and heat preservation when filling, and circulation pipeline builds not only convenient fixed around constant temperature water bath, can simplify pipe-line system to a certain extent simultaneously, reduces the use of connector, and constant temperature water bath can freely select for use according to the laboratory condition for the size, and the consumptive material economy obtains the facility.
(3) The disposable aseptic heparin cap is connected with the three-way pipe A, a sample can be slowly extracted through the heparin cap by using a 1ml aseptic syringe in the perfusion process, and the influence on the pressure and the system stability is greatly reduced because the three-way pipe does not need to be opened and closed at any time due to good tightness of the heparin cap.
(4) The organ storage pool is a cubic quartz vessel, and combines the organ bin with the venous pool, so that redundant pipeline connection is reduced, pre-filling is reduced, space is saved, the temperature and the humidity of the kidney can be guaranteed, and the organ storage pool can be repeatedly used in high-temperature and high-pressure sterilization.
(5) The rat kidney is small, the external perfusion flow is not too large, the required oxygen flow is small, the range of the oxygen flow meter used conventionally is too large, and the device adopts a Y-shaped pipe and a flow regulator to finely regulate the oxygen content of the perfusion blood.
(6) The utility model discloses simple structure, easy and simple to handle, equipment are general, can improve rat kidney preservation quality, extension save time.
Drawings
Fig. 1 is a schematic view of the whole structure of the rat kidney normal temperature mechanical preservation and re-irrigation system of the utility model;
FIG. 2 is a front view of the organ reservoir according to the present invention;
in the figure: 1-micro-injection pump, 2-organ storage pool, 3-ureter cannula, 4-urine collecting tube, 5-peristaltic pump, 6-pressure sensor, 7-sterile tube, 8-computer detector, 9-pressure detection system, 10-oxygenator, 11-flow regulator, 12-Y tube, 13-oxygen bottle, 14-heparin cap, 15-dropping funnel, 16-three-way tube B, 17-constant temperature water bath, 18-three-way tube A, 19-oxygen tube, 20-round hole M (outlet), 21-urine guide hole, 22-thin layer perforated plate, 23-round hole N, 24-organ bin, 25-venous pool, 26-inlet.
Detailed Description
The following description is given with reference to the accompanying drawings and the detailed description, but not to limit the present invention. The technical features described in the following embodiments may be combined with each other as long as they do not conflict with each other.
As shown in fig. 1, a rat kidney normal temperature mechanical preservation and re-irrigation system comprises a preservation re-irrigation device, an oxygen supply device, a control device, a micro-injection pump 1 and a constant temperature water bath 17. The preservation and refilling device is a circulating pipeline device which is formed by connecting an organ storage pool 2, a peristaltic pump 5, an oxygenator 10, a dropping funnel 15 and a pressure receptor 6 sequentially through a sterile pipeline 7. The outlet of the organ storage pool 2 is connected with the inlet end of a peristaltic pump 5 through a sterile pipeline 7, the outlet end of the peristaltic pump 5 is connected with the inlet end of an oxygenator 10 through the sterile pipeline 7, a three-way pipe A is arranged on the sterile pipeline 7 between the oxygenator 10 and a dropping funnel 15, the inlet end of the three-way pipe A is connected with the outlet end of the oxygenator 10, one outlet end of the three-way pipe A is connected with the inlet end of the dropping funnel 15, the other outlet end of the three-way pipe A is provided with a heparin cap 14, a three-way pipe B is arranged on the sterile pipeline 7 between the dropping funnel 15 and a pressure receptor 6, the inlet end of the three-way pipe B is connected with the outlet end of the dropping funnel 15, one outlet end of the three-way pipe B is connected with the inlet end of the pressure receptor 6, the other outlet end, the organ reservoir 2, oxygenator 10, drip chamber 15, baroreceptor 6, and sterile tubing between the oxygenator 10 and the organ reservoir 2 are all located in a thermostatted water bath 17. The arterial circulation pipeline and the organ storage pool 2 are both immersed in the constant-temperature water bath 17, so that the pipeline and the organ storage pool 2 are synchronously reheated and insulated during perfusion. The micro-syringe pump 1 is used for continuous infusion of drugs into the returning arterial blood during the preservation phase. The constant-temperature water bath pot displays the heating temperature of the perfusate in real time. The peristaltic pump 5 monitors the inflow to the kidney in real time.
The oxygen supply device is a pipeline device which is composed of an oxygen bottle 13, a Y-shaped pipe 12 and a flow regulator 11 sequentially through an oxygen pipeline 19, the outlet end of the oxygen bottle 13 is connected with the inlet end of the Y-shaped pipe 12 through the oxygen pipeline 19, the outlet end of the Y-shaped pipe 12 is connected with the inlet end of the flow regulator 11, and the outlet end of the flow regulator 11 is connected into the oxygenator 10. I.e. the outlet end of the oxygen supply device is connected into said oxygenator 10. The oxygen content in the oxygenator 10 is adjusted by means of the Y-tube 12 and the flow regulator 11 according to the content of the perfusate.
The control device comprises a pressure detection system 9 and a computer detector 8 connected with the pressure detection system, wherein the pressure detection system 9 is connected with the signal transmission end of the baroreceptor 6 to monitor the renal artery pressure in real time.
As shown in fig. 2, the organ storage pool 2 is a closed chamber, and a top cover plate of the organ storage pool is provided with a round hole M and a urethral orifice 21, the round hole M is an outlet of the organ storage pool 2, the organ storage pool 2 is transversely provided with a thin layer perforated plate 22 which separates the organ storage pool 2 into an upper chamber and a lower chamber, the upper chamber is an organ chamber 24, the organ chamber 24 is used for placing the kidney of a rat, the lower chamber is a vein pool 25, and the vein pool 25 is used for storing venous blood. The thin-layer perforated plate 22 is provided with a plurality of perforated holes, round holes N with corresponding apertures are formed in the positions, corresponding to the round holes M of the top cover plate, of the thin-layer perforated plate 22, and the bottom of the venous pool 25 is of a V-shaped structure with high side wall and low center. Blood can be collected in the minimum through this design, avoids causing the erythrocyte sedimentation in the perfusate of other places because pipeline local suction perfusate. One end of the sterile pipeline 7 passes through a circular hole M on the top cover plate and then extends into the venous pool through a circular hole N on the thin-layer perforated plate 22, and the other end of the sterile pipeline is connected with the peristaltic pump 5, so that the flow is monitored in real time through the peristaltic pump 5. The side wall of the organ bin 24 is provided with an inlet 26 of the organ storage pool 2, the outlet end of the baroreceptor 6 is connected to the inlet 26, and the baroreceptor enters the organ bin 24 to be further connected with the renal artery. The external ureteral cannula 3 of establishing of catheterization hole 21, ureteral cannula 3 terminal is equipped with urine collecting pipe 4 for collect the urine.
The urine collecting pipe 4 is detachably adhered to the outer side wall of the constant-temperature water bath 17. The tape fixing or other sticking methods can be adopted. The urine collection tube may be a sterile EP tube.
The inlet end of the drip chamber 15 is higher than the outlet end thereof. To ensure that arterial blood is bubble free.
The sterile pipeline 7 is a disposable medical rubber hose.
The organ storage pool can be a cubic quartz vessel and can be reused after being sterilized at high temperature and high pressure.
The working principle is as follows: before use, the fume hood is sterilized in advance, and the organ storage tank is autoclaved at the same time. When in use, a rat kidney normal-temperature mechanical preservation and re-perfusion system is connected in a fume hood by adopting a sterile pipeline 7 according to the connection mode, a rat kidney is placed in an organ bin 24, prepared venous blood is injected along a round hole M on a top cover plate by using a small-range disposable sterile injector, flows through a leak hole on a thin-layer leak hole plate 22 and enters a venous pool 25, the tail end of the sterile pipeline 7 connected with the inlet end of a peristaltic pump 5 passes through the round hole M and extends into venous blood in the venous pool 25 through a round hole N on the thin-layer leak hole plate 22, the peristaltic pump is started, a constant-temperature water bath kettle is opened to set the temperature to 36-37 ℃, blood re-warming and oxygenation are carried out on the venous pool 24 and a circulation pipeline partially positioned in the constant-temperature water bath kettle for 20min, then a rat renal artery is punctured by using a pressure needle, the renal artery indwelling needle is emptied and then is connected with the sterile pipeline 7 connected with an inlet 26 by a pressure indwelling sensor 6, opening the three-way pipe B, starting the micro-injection pump 1, administering protective medicine to the backflow arterial blood, rotating a knob of the peristaltic pump 5, gradually adjusting the flow, and monitoring the pressure through a computer to stabilize the pressure at 65-75 mmHg. And (3) ending the storage stage, taking down the kidney, placing the kidney in ice to flush out residual blood in the kidney, cleaning a circulation passage by using sterile physiological saline, injecting prepared venous blood for repeated perfusion into the venous pool along the circular hole M on the top cover plate by using a small-range disposable sterile injector, closing the three-way pipe B, extending the tail end of the sterile pipeline 7 connected with the inlet end of the peristaltic pump 5 into the venous blood in the venous pool 25 through the circular hole M and the circular hole N on the thin-layer perforated plate 22, starting the peristaltic pump, oxygenating for 20min, connecting the kidney, and controlling the pressure to be 75-85 mmHg.
The utility model discloses rat kidney normal atmospheric temperature machinery is preserved and system in use is irritated again specifically the operation as follows:
when the kidney of the rat is preserved, injecting the prepared blood for preserving the kidney into a venous pool by a disposable sterile injector through a round hole M on a top cover plate; starting the peristaltic pump 5, adjusting the constant-temperature water bath to heat the perfusate, and controlling the perfusion temperature to be 35-37 ℃; opening the three-way pipe B, starting the micro-injection pump, and administering a protective drug to the blood which flows back to the renal artery through the sterile pipeline; starting an oxygenation device, controlling the flow of oxygen, removing bubbles in a pipeline, and measuring blood gas analysis indexes in the initial perfusate after 20 min; the kidney artery indwelling needle is connected with the pressure receptor, the kidney is placed in the organ bin, and the perfusate is driven by the peristaltic pump to perfuse the kidney after the erythrocyte is carried with oxygen through the oxygenator and the dropping funnel. Urine flows into the urine collection device through the ureteral cannula. The pressure monitoring system detects the pressure of the renal artery in real time, and the pressure is maintained at 65-75mmHg during the perfusion process. After the storage time is reached, the kidneys are removed and placed on sterile ice, the kidneys and the perfusion circulation system are flushed until no blood and impurities remain, and preparation is made for re-perfusion.
When the rat kidney is refilled, the three-way pipe B connected with the micro-injection pump is closed, refilling liquid is prepared from the last hour of the storage stage, the refilling liquid is added into the venous pool by using a disposable sterile injector after a perfusion circulation system is cleaned, and the subsequent steps are consistent with the storage stage. When the storage period is different from the preservation period, the step-by-step 30min rewarming is not carried out after the kidney is connected, and the normal-temperature rewetting is directly carried out within the range of 75-90mmHg perfusion pressure. Sampling from the heparin cap and the renal vein port at the beginning of re-perfusion and every hour after the beginning, reserving and taking a perfusate sample, and carrying out corresponding blood gas, physiological and biochemical detection and the like. And after the re-perfusion time is reached, closing the peristaltic pump to stop perfusion, preparing a sterile culture dish, laying a layer of sterile crushed ice and gauze, taking down the kidney, placing the kidney on the gauze, and flushing the kidney by using normal saline containing heparin. Cutting kidney tissue, and performing detection such as staining and biochemistry.
The utility model discloses in, except that except repeated use after going out the organ storage pond through high temperature autoclaving, all the other most consumptive materials are disposable aseptic consumptive materials, greatly reduce the pollution probability, connect portably, conveniently dismantle change and transportation. Partial circulation pipeline, vein pond all submerge in constant temperature water bath, have guaranteed that pipeline and vein pond are synchronous rewarming and heat preservation when filling, and circulation pipeline builds not only convenient fixed around constant temperature water bath, can simplify pipe-line system to a certain extent simultaneously, reduces the use of connector, and constant temperature water bath can freely select for use according to the laboratory condition for the size, and the consumptive material economy obtains the facility. The disposable aseptic heparin cap is connected with the three-way pipe A, a sample can be slowly extracted through the heparin cap by using a 1ml aseptic syringe in the perfusion process, and the influence on the pressure and the system stability is greatly reduced because the three-way pipe does not need to be opened and closed at any time due to good tightness of the heparin cap. The organ storage pool is a cubic quartz vessel, and combines the organ bin with the venous pool, so that redundant pipeline connection is reduced, pre-filling is reduced, space is saved, the temperature and the humidity of the kidney can be guaranteed, and the organ storage pool can be repeatedly used in high-temperature and high-pressure sterilization. The rat kidney is small, the external perfusion flow is not too large, the required oxygen flow is small, the range of the oxygen flow meter used conventionally is too large, and the device adopts a Y-shaped pipe and a flow regulator to finely regulate the oxygen content of the perfusion blood.
The foregoing is a preferred embodiment of the present invention, and the scope of the present invention should not be limited thereby, and it should be understood that modifications and variations can be made by those skilled in the art without departing from the principles of the present invention, and such modifications and variations are also considered to be within the scope of the present invention.
Claims (9)
1. The utility model provides a rat kidney normal atmospheric temperature machinery is preserved and system of irritating again which characterized in that: comprises a preservation and re-filling device, an oxygen supply device, a control device, a micro-injection pump (1) and a constant temperature water bath (17); the preservation and refilling device is a circulating pipeline device which is formed by connecting an organ storage pool (2), a peristaltic pump (5), an oxygenator (10), a dropping funnel (15) and a pressure receptor (6) in sequence through a sterile pipeline (7), the outlet of the organ storage pool (2) is connected with the inlet end of the peristaltic pump (5) through the sterile pipeline (7), the outlet end of the peristaltic pump (5) is connected with the inlet end of the oxygenator (10) through the sterile pipeline (7), a three-way pipe A (18) is arranged on the sterile pipeline (7) between the oxygenator (10) and the dropping funnel (15), the inlet end of the three-way pipe A (18) is connected with the outlet end of the oxygenator (10), one outlet end of the three-way pipe A (18) is connected with the inlet end of the dropping funnel (15), the other outlet end of the three-way pipe A is provided with a heparin cap (14), and a three-way pipe B (16) is arranged on the sterile pipeline (7) between the dropping funnel (, the inlet end of the three-way pipe B (16) is connected with the outlet end of a dropping funnel (15), one outlet end of the three-way pipe B (16) is connected with the inlet end of a pressure receptor (6), the other outlet end of the three-way pipe B (16) is connected with a micro-injection pump (1) through a sterile pipeline (7), the outlet end of the pressure receptor (6) is connected with the inlet end of an organ storage pool (2), and the organ storage pool (2), an oxygenator (10), the dropping funnel (15), the pressure receptor (6) and the sterile pipeline (7) between the oxygenator (10) and the organ storage pool (2) are all positioned in a constant temperature water bath (17); the outlet end of the oxygen supply device is connected into the oxygenator (10); the control device is connected with the signal transmission end of the pressure sensor (6).
2. The rat kidney normothermic mechanical preservation and recharge system of claim 1, wherein: the oxygen supply device is a pipeline device which is composed of an oxygen bottle (13), a Y-shaped pipe (12) and a flow regulator (11) sequentially through an oxygen pipeline (19), the outlet end of the oxygen bottle (13) is connected with the inlet end of the Y-shaped pipe (12), the outlet end of the Y-shaped pipe (12) is connected with the inlet end of the flow regulator (11), and the outlet end of the flow regulator (11) is connected into the oxygenator (10).
3. The rat kidney normothermic mechanical preservation and recharge system of claim 1, wherein: the control device comprises a pressure detection system (9) and a computer detector (8) connected with the pressure detection system, wherein the pressure detection system (9) is connected with a signal transmission end of the pressure sensor (6).
4. The rat kidney normothermic mechanical preservation and recharge system of claim 1, wherein: the organ storage pool (2) is a closed cavity, a top cover plate of the organ storage pool is provided with a round hole M (20) and a urethral catheterization hole (21), the organ storage pool (2) is transversely provided with a thin-layer perforated plate (22) for dividing the organ storage pool (2) into an upper cavity and a lower cavity, the upper cavity is an organ bin (24), the lower cavity is a vein pool (25), the thin-layer perforated plate (22) is provided with a plurality of perforated holes, a round hole N (23) with a corresponding hole diameter is arranged on the thin-layer perforated plate (22) at a position corresponding to the round hole M (20) of the top cover plate, the bottom of the vein pool (25) is of a V-shaped structure with a high side wall and a low center, and the side wall of the organ bin (24) is provided with an inlet; the round hole M (20) is an outlet of the organ storage pool (2), the ureter casing (3) is externally connected to the urethral catheterization hole (21), and the tail end of the ureter casing (3) is provided with a urine collecting pipe (4).
5. The rat kidney normothermic mechanical preservation and recharge system of claim 4, wherein: the urine collecting pipe (4) is detachably adhered to the outer side wall of the constant-temperature water bath kettle (17).
6. The rat kidney cold mechanical preservation and re-perfusion system according to claim 4 or 5, characterized in that: the urine collecting pipe is a sterile EP pipe.
7. The rat kidney normothermic mechanical preservation and recharge system of claim 1, wherein: the inlet end of the dropping funnel (15) is higher than the outlet end thereof.
8. The rat kidney normothermic mechanical preservation and recharge system of claim 1, wherein: the sterile pipeline (7) is a disposable medical rubber hose.
9. The rat kidney normothermic mechanical preservation and recharge system of claim 1, wherein: the organ storage pool (2) is a cubic quartz vessel and can be reused after high-temperature and high-pressure sterilization.
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| CN202020639775.4U CN213099890U (en) | 2020-04-24 | 2020-04-24 | Normal-temperature mechanical preservation and re-irrigation system for rat kidney |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115885972A (en) * | 2022-10-24 | 2023-04-04 | 中国科学院理化技术研究所 | Organ perfusion preservation equipment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115885972A (en) * | 2022-10-24 | 2023-04-04 | 中国科学院理化技术研究所 | Organ perfusion preservation equipment |
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