CN115885972A - Organ perfusion preservation equipment - Google Patents
Organ perfusion preservation equipment Download PDFInfo
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- CN115885972A CN115885972A CN202211305787.3A CN202211305787A CN115885972A CN 115885972 A CN115885972 A CN 115885972A CN 202211305787 A CN202211305787 A CN 202211305787A CN 115885972 A CN115885972 A CN 115885972A
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Abstract
The invention provides perfusion preservation equipment for organs, which comprises a storage device, a liquid filling device and a temperature control device, wherein the temperature control device is connected with the storage device; the storage device comprises a placing bin, a placing cavity for placing organs is formed in the placing bin, and a liquid inlet and a liquid outlet are arranged through the placing cavity; the liquid filling device comprises a liquid storage tank, a liquid filling pump and a circulating pipeline, the circulating pipeline comprises a liquid inlet pipeline and a liquid outlet pipeline communicated to the liquid storage tank, and the liquid filling pump is arranged on the circulating pipeline; the temperature control device is used for controlling the temperature of the liquid storage tank; the liquid inlet pipeline is communicated to the liquid outlet, and the liquid outlet pipeline is communicated to the liquid inlet. In the invention, the storage device and the liquid filling device jointly form a circulating system, so that the circulating filling of the organ is realized, the accumulation of metabolites in the organ is avoided, and meanwhile, the temperature of the liquid storage tank is controlled by the temperature control device, so that the cold storage with longer time and higher quality is realized.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to perfusion and preservation equipment for organs.
Background
Due to the deep understanding of human body structure, the development of surgical operation technology and the research and development and application of related immunosuppressive drugs, organ transplantation operations such as kidney, liver, heart, lung and the like have been successfully implemented in the medical field, and the relay of life is realized; although great progress has been made in the level of intraoperative surgical procedures and postoperative immune rejection, further development of organ transplantation is still limited by a number of preoperative factors, such as preservation of ex vivo organs, irreversible damage to many organs due to inadequate preservation, forced discarding without reaching the standard of transplantation, further exacerbating the situation of organ shortage.
Currently, after harvesting donor organs, the organs are rapidly perfused with a lavage fluid, allowed to cool uniformly to below 10 ℃, and stored statically cold until transplantation surgery is performed. The static cold preservation operation is simple, no additional equipment is needed, and the preservation requirement of standard donor organs with good quality can be met. However, the storage time of static cold storage is short, the storage process is not controlled, the temperature change is large, the evaluation of the stored organs cannot be carried out, and the like.
Disclosure of Invention
The invention provides an organ perfusion preservation device, which is used for solving the problem that organs are difficult to preserve statically and coldly and realizing long-time effective preservation of the organs.
The invention provides an organ perfusion preservation device, comprising:
the storage device comprises a placing bin, wherein a placing cavity for placing an organ is formed in the placing bin, and a liquid inlet and a liquid outlet are arranged in the placing cavity in a penetrating manner;
the liquid filling device comprises a liquid storage tank, a liquid filling pump and a circulating pipeline, wherein the circulating pipeline comprises a liquid inlet pipeline and a liquid outlet pipeline communicated to the inside of the liquid storage tank, and the liquid filling pump is arranged on the circulating pipeline; and the number of the first and second groups,
the temperature control device is used for controlling the temperature of the liquid storage tank;
the liquid inlet pipeline is communicated to the liquid outlet, and the liquid outlet pipeline is communicated to the liquid inlet.
According to the organ perfusion preservation equipment provided by the invention, the air outlet is also arranged in the placing cabin in a penetrating way.
According to the organ perfusion preservation equipment provided by the invention, the storage device further comprises a waste liquid temporary storage bin, wherein a waste liquid cavity is formed in the waste liquid temporary storage bin and communicated to the arrangement cavity; and/or the presence of a gas in the atmosphere,
the storage device also comprises a secretion collecting bin and a collecting hose communicated to the secretion collecting bin, and the free end of the collecting hose is communicated to the placing cavity and used for collecting secretions on organs.
According to the organ perfusion preservation equipment provided by the invention, the liquid storage tank is also provided with a drug adding and fluid supplementing port in a penetrating way.
According to the organ perfusion preservation equipment provided by the invention, the temperature control device comprises a compressor, a condenser and an evaporator which are mutually connected;
wherein, the evaporator is arranged at the outer side of the liquid storage tank.
According to the organ perfusion preservation equipment provided by the invention, the temperature control device further comprises a four-way valve, and the four-way valve is arranged among the compressor, the condenser and the evaporator and used for switching the trend of cooling liquid in the compressor; and/or the presence of a gas in the atmosphere,
the temperature control device further comprises a capillary tube, and the capillary tube is arranged between the condenser and the evaporator.
According to the organ perfusion preservation equipment provided by the invention, the organ perfusion preservation equipment further comprises a monitoring device, wherein the monitoring device comprises a liquid level sensor which is arranged in the placing bin and used for detecting the height of the liquid level in the placing bin; and/or the presence of a gas in the gas,
the monitoring device also comprises a flow sensor which is arranged on the circulating pipeline and used for detecting the liquid filling flow in the circulating pipeline; and/or the presence of a gas in the atmosphere,
the monitoring device also comprises a pressure sensor which is arranged on the circulating pipeline and is used for detecting the conveying pressure in the circulating pipeline; and/or the presence of a gas in the gas,
the monitoring device further comprises a temperature sensor mounted on the circulating pipeline and used for detecting the temperature in the circulating pipeline.
According to the organ perfusion preservation equipment provided by the invention, the organ perfusion preservation equipment further comprises a filtering device, the filtering device is installed in the circulating pipeline, and the filtering device comprises at least one of a bubble filter, a thrombus filter and a leukocyte filter.
According to the present invention there is provided an organ perfusion preservation apparatus, further comprising oxygenation means, the oxygenation means comprising:
the oxygenator is communicated into the liquid inlet pipeline and is used for maintaining oxygen saturation; and the number of the first and second groups,
an oxygen bottle communicated to the oxygenator for providing oxygen to the oxygenator.
According to the organ perfusion preservation apparatus provided by the present invention, the oxygenation device further comprises:
a secondary circulation pipeline comprising a first inlet, a first outlet and a second outlet, wherein the first inlet is communicated to the oxygenator, the first outlet is communicated to the placing bin, and the second outlet is communicated to the liquid inlet pipeline; and the number of the first and second groups,
the blood gas analysis unit is arranged between the secondary circulation pipeline and the liquid inlet pipeline and used for detecting the oxygen saturation difference between the secondary circulation pipeline and the circulation pipeline;
wherein, all be provided with control flap on first export and the second export.
In the organ perfusion preservation equipment provided by the invention, the storage device and the perfusion device jointly form a circulating system, perfusion liquid in the liquid storage tank flows into the placing bin through the liquid outlet pipeline under the action of the perfusion pump and flows back into the liquid storage tank again through the liquid inlet pipeline, so that the circulating perfusion of the organ is realized, enough metabolic substrates are provided for the organ, the accumulation of metabolic products in the organ is avoided, meanwhile, the temperature of the liquid storage tank is controlled through the temperature control device, the temperature of the perfusion liquid in the liquid storage tank is further controlled and reduced, and the cold preservation with longer time and higher quality is realized.
Drawings
In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.
Fig. 1 is a schematic view of a connection structure of an organ perfusion preservation apparatus 100 according to the present invention.
Reference numerals:
100. organ perfusion preservation equipment; 11. arranging a bin; 111. an exhaust port; 12. temporarily storing the waste liquid in a bin; 13. a secretion collection bin; 21. a liquid storage tank; 211. a medicine feeding and fluid supplementing port; 22. a liquid filling pump; 23. a circulation pipe; 31. a compressor; 32. a condenser; 33. an evaporator; 41. a liquid level sensor; 42. a flow sensor; 43. a pressure sensor; 44. a temperature sensor; 51. a bubble filter; 52. a thrombus filter; 53. a leukocyte filter; 61. an oxygenator; 62. an oxygen cylinder; 63. a secondary circulation pipeline; 64. a blood gas analysis unit.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.
Referring to fig. 1, the present invention provides an organ perfusion preservation apparatus 100, which includes a storage device, a liquid filling device, and a temperature control device; the storage device comprises a placing bin 11, wherein a placing cavity for placing organs is formed in the placing bin 11, and a liquid inlet and a liquid outlet are formed through the placing cavity; the liquid filling device comprises a liquid storage tank 21, a liquid filling pump 22 and a circulating pipeline 23, the circulating pipeline 23 comprises a liquid inlet pipeline and a liquid outlet pipeline which are communicated to the liquid storage tank, and the liquid filling pump 22 is arranged on the circulating pipeline 23; the temperature control device is used for controlling the temperature of the liquid storage tank 21; wherein, the liquid inlet pipeline is communicated to the liquid outlet, and the liquid outlet pipeline is communicated to the liquid inlet.
In the organ perfusion preservation device 100 provided by the invention, the storage device and the perfusion device together form a circulating system, the perfusion liquid in the liquid storage tank 21 flows into the placing bin 11 and the organ through the liquid outlet pipeline under the action of the perfusion pump 22, and flows back to the liquid storage tank 21 again through the liquid inlet pipeline, so that the circulating perfusion of the organ is realized, sufficient metabolic substrates are provided for the organ, the accumulation of metabolic products in the organ is avoided, meanwhile, the temperature of the liquid storage tank 21 is controlled through the temperature control device, the temperature of the perfusion liquid in the liquid storage tank 21 is further controlled and reduced, and the cold preservation with longer time and higher quality is realized.
It should be noted that, in this embodiment, the manner of perfusing perfusate into an organ is called mechanical perfusion, and in this embodiment, metabolic substrates are supplied to the organ and metabolic waste products are timely transported out, and various drugs can be added to assist in repairing the organ, and at the same time, perfusate and secretion of the organ can be analyzed, and organ quality can be more effectively evaluated; meanwhile, the supercooling mechanical perfusion can prolong the preservation time of the organ, avoid the damage of crystallization to the organ, ensure the long-distance transfer of the organ and the quality of the transplanted organ, and simultaneously, further relieve the cold preservation damage inevitably generated by low-temperature preservation.
In addition, the perfusate is provided with various embodiments, which aim to provide necessary metabolic substrates and survivable substances for the organ on the basis of realizing temperature reduction, and in the embodiment, the perfusate comprises UW liquid.
It should be noted that, in this embodiment, the temperature control device controls the temperature of the perfusate in the liquid storage tank 21 to be 4-6 ℃, during the perfusion process of the actual organ, the organ is usually uniformly cooled to below 10 ℃ before the isolated organ is placed in the perfusion preservation apparatus 100, and by controlling the temperature to be 4-6 ℃, the effective perfusion of the organ is realized, so as to form low-temperature perfusion preservation, and realize longer-time and higher-quality cold preservation.
Furthermore, in order to further inhibit the metabolism of organs through low temperature, supercooling mechanical perfusion can be carried out, and on the basis of the low temperature perfusion, the temperature is gradually adjusted to-6 ℃ to realize the supercooling perfusion.
It should be noted that, in this embodiment, in order to ensure safe use of the organ perfusion preservation apparatus 100, the material of the fluid reservoir 21 includes a stainless steel material, which facilitates heat conduction on the premise of ensuring safety.
In addition, in order to improve the temperature control efficiency of the reservoir tank 21 for the internal perfusion fluid, a plurality of heat conduction fins are formed on the inner wall surface of the reservoir tank 21.
Further, an air outlet 111 is further disposed through the installation bin 11. When in use, the air in the placing cabin 11 is pumped out through the air outlet 111, so that the organs are protected and the outside air pollution is isolated. The exhaust port is provided with an exhaust part which is sterilized by polyethylene, so that the antibacterial performance and the use safety are ensured.
On the other hand, the storage device further comprises a waste liquid temporary storage bin 12, wherein a waste liquid cavity is formed in the waste liquid temporary storage bin 12 and communicated to the arrangement cavity. In this embodiment, waste liquid temporary storage storehouse 12 communicates to settling storehouse 11 in, at the perfusate at storage device and irritate the in-process of liquid device between the circulation, can produce the effective material inefficacy gradually, and dissolved too much metabolite's perfusate, forms heavier waste liquid, derives the waste liquid through waste liquid temporary storage storehouse 12, avoids it to circulate in the circulation system always, leads to the preservation life-span of organ to shorten, and it is long when improving the preservation.
Similarly, the storage device further comprises a secretion collecting bin 13 and a collecting hose communicated to the inside of the secretion collecting bin 13, wherein the free end of the collecting hose is communicated to the placing cavity for collecting the secretion on the organ. In this embodiment, the organ is preserved in the placing cabin 11, and its work and metabolism are slowed down through cryogenic mode, however even in the condition of slowing down, work secretion still can be produced to partial organ, in this embodiment, is connected to the organ through collecting the hose to collect the secretion that the organ produced, avoid the secretion to pollute the perfusate, improve and preserve for a long time.
Further, be provided with waste liquid level monitoring device in the storehouse 13 is collected to the secretion to detect the waste liquid how much.
It should be noted that, in the setting of the two related technical features of the waste liquid temporary storage bin 12 and the secretion collection bin 13, they may exist alternatively or simultaneously, and in the present invention, there is no specific limitation, and the setting is performed according to the actual storage requirement and the storage time, and obviously, the two features exist simultaneously, which results in the longest storage time.
On the other hand, a drug-adding and fluid-supplementing port 211 is further provided through the reservoir 21. According to different organs, temperatures, midway losses and the like, the perfusate is changed during actual perfusion, in the embodiment, the perfusate is supplemented into the liquid storage tank 21 through the medicine adding and liquid supplementing port 211, and the liquid medicine is supplemented according to the requirement so as to ensure the preservation of the organs.
When different organs are targeted, different medicines are added into the UW liquid through the medicine adding and fluid supplementing port 211, for example, insulin, PEG, 3-OMG, trehalose, proline and the anti-inflammatory dexamethasone are added into the UW liquid in a targeted manner during the preservation process of the liver and the refrigeration process
Further, the temperature control device comprises a compressor 31, a condenser 32 and an evaporator 33 which are connected with each other; wherein, the evaporator 33 is arranged outside the liquid storage tank 21. In the present embodiment, a temperature reducing system is formed by the compressor 31, the condenser 32, and the evaporator 33, so that the coolant evaporates and absorbs heat at the evaporator 33 by circulation of the coolant to reduce the temperature of the reservoir tank 21.
It should be noted that the evaporator only needs to be capable of exchanging temperature with the liquid storage tank 21, for example, the evaporator 33 is attached to the outer side wall of the liquid storage tank 21, in the embodiment of the present invention, the evaporator 33 is disposed in a spiral pipe, and the evaporator 33 is disposed around the liquid storage tank 21, so as to exchange temperature.
Further, the temperature control device further includes a four-way valve installed between the compressor 31, the condenser 32 and the evaporator 33 for switching the direction of the cooling liquid in the compressor 31. In this embodiment, through the setting of four-way valve for temperature control device can carry out the evaporation heat absorption and the condensation is exothermic in evaporimeter 33 department, realizes the cooling and the intensification to liquid storage pot 21, is convenient for to the preservation and the multiplexing of organ, the multiplexing device that changes when need not multiplexing again.
In the embodiment provided by the invention, the four-way valve is used for switching the warming effect at the evaporator 33, when the organ is rewarmed before transplantation, the supercooling perfusion stage is firstly separated, then the low-temperature perfusion and the sub-normal-temperature perfusion are carried out for a period of time, and finally the normal-temperature perfusion is gradually recovered to 32-37 ℃ for gradually recovering the organ.
In addition, during the warming process, insulin, dexamethasone, streptomycin, antioxidant, heparin, 25% human albumin, proline and trehalose are supplemented into the perfusate through the drug-adding and fluid-supplementing port 211.
Before the normal-temperature mechanical perfusion is carried out, diluted blood and platelets are added into the perfusate through the medicine adding and fluid supplementing port 211, and dexamethasone and streptomycin should be supplemented again to avoid the problems of higher bacterial infection, inflammation and the like at higher temperature.
In this example, the sub-cooling perfusion is performed at-6 ℃, the low-temperature mechanical perfusion is performed at 4-6 ℃, the sub-normal-temperature mechanical perfusion is performed at 20 ℃, and the normal-temperature mechanical perfusion is performed at 32-37 ℃.
The temperature control device further includes a capillary tube disposed between the condenser 32 and the evaporator 33. Throttling and internal refrigerant pressure regulation are performed by a capillary tube.
It should be noted that, in the arrangement of the four-way valve and the capillary, they may exist either or both, and are not limited specifically again.
It should be noted that the temperature control principle of the temperature control device formed by the compressor 31, the condenser 32 and the evaporator 33 is clear to those skilled in the temperature control field, i.e. heat absorption by evaporation and heat release by condensation, and will not be described herein again.
The temperature of the compressor 31, the condenser 32, and the evaporator 33 is controlled by a refrigerant, which in this embodiment is R134a, whose main component is tetrachloromethane, and which has a boiling point of-26.1 ℃ at normal pressure and a critical temperature of 101.1 ℃, and is a non-toxic and non-flammable safety refrigerant.
Specifically, in this embodiment, the temperature is reduced to the supercooling temperature by a step-type cooling method, long-term preservation is realized by continuous perfusion, the organ vitality can be warmed and restored by step-type rewarming before organ transplantation, and the function of operating in multiple perfusion modes is realized by using the characteristics of a temperature-adjustable system.
On the other hand, the organ perfusion preservation apparatus 100 further comprises a monitoring device, which includes a liquid level sensor 41 installed in the installation chamber for detecting the level of the liquid in the installation chamber.
Similarly, the monitoring device further includes a flow sensor 42 mounted to the circulation line 23 for detecting the flow rate of the irrigation liquid in the circulation line 23.
Likewise, the monitoring device also comprises a pressure sensor 43 mounted to the circulation conduit 23 for detecting the delivery pressure in the circulation conduit 23.
Similarly, the monitoring device further includes a temperature sensor 44 mounted to the circulation duct 23 for detecting the temperature in the circulation duct 23.
The liquid level sensor 41, the flow rate sensor 42, the pressure sensor 43, and the temperature sensor 44 may be provided in any one or a plurality of them, or may be provided in combination, and the present invention is not particularly limited.
On the other hand, the organ perfusion preservation apparatus 100 further includes a filter device installed into the circulation duct 23, the filter device including at least one of the bubble filter 51, the thrombus filter 52, and the leukocyte filter 53. Thereby filtering the impurities in the perfusate.
In the present embodiment, the air bubble filter 51, the thrombus filter 52, and the leukocyte filter 53 are provided in this order along the flow direction of the perfusion fluid.
Specifically, through the bubble in the bubble filter 51 filtration perfusate, avoid causing the damage to the organ, guarantee simultaneously that the certain liquid filling volume of bubble filter 51 plays the cushioning effect, make the system operation more stable, reduce the pressure and the flow fluctuation of system.
Also, the thrombus filter 52 is disposed downstream of the bubble filter 51 to prevent the substance micelles in the perfusate from depositing in the organ and causing circulatory disturbance of the organ.
Similarly, the leukocyte filter 53 is located downstream of the thrombus filter 52, and when perfusion is performed at a higher temperature, leukocytes in diluted blood are removed, thereby avoiding problems such as immune reaction and the like, which may cause damage to the preserved organs.
On the other hand, the organ perfusion preservation apparatus 100 further comprises an oxygenating device, which comprises an oxygenator 61 and an oxygen cylinder 62; the oxygenator 61 is communicated to the liquid inlet pipeline and is used for maintaining oxygen saturation; an oxygen cylinder 62 is connected to the oxygenator 61 for supplying oxygen to the oxygenator 61. In this embodiment, the oxygen saturation within the perfusate is maintained by the oxygenator 61.
It should be noted that the oxygenation device further comprises a regulating valve to facilitate accurate regulation of the oxygen concentration by the valve.
Further, the oxygenation device further comprises a secondary circulation pipeline 63 and a blood gas analysis unit 64; the secondary circulation pipeline 63 comprises a first inlet, a first outlet and a second outlet, wherein the first inlet is communicated to the oxygenator 61, the first outlet is communicated to the placing bin 11, and the second outlet is communicated to the liquid inlet pipeline; a blood gas analyzing unit 64 is installed between the secondary circulation pipe 63 and the liquid inlet pipe to detect the difference in oxygen saturation between the secondary circulation pipe 63 and the circulation pipe 23; wherein, all be provided with control flap on first export and the second export. In this embodiment, through inferior circulating line, realize settling the two-way perfusion in the storehouse 11, be convenient for realize two-way perfusion to different organs, guarantee the stable cooling of organ.
The secondary circulation pipe 63 is further provided with a second flow rate sensor and a second pressure sensor. To monitor the flow and pressure in the secondary loop line 63.
Specifically, in this embodiment, in the initial cooling process of organ perfusion preservation equipment 100, circulating line and inferior circulating line all are the circulation to inside cooling down rapidly, after reaching preset temperature, close the valve on the second export, make organ perfusion preservation equipment 100 form two circulation flow paths in, maintain the organ in the specific perfusion state of perfusion in-process, avoid too big pressure, flow and temperature variation to cause the harm to liver endothelial cell.
In addition, the blood gas analyzing unit 64 functions as follows: the blood gas analyzer 64 collects and analyzes the perfusate from a first outlet of the perfusate connected with the inferior vena cava and an oxygenated pipeline, on one hand, the blood gas analyzer 64 can be matched with an oxygenation unit to monitor the oxygen saturation in the perfusate so as to maintain the blood oxygen saturation at about 95%, and meanwhile, the blood gas analyzer 64 can also analyze and monitor the pH value of the perfusate, and when the pH value is too low, the pH value can be adjusted to 7.3-7.4 by adding sodium bicarbonate.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. An organ perfusion preservation apparatus, comprising:
the storage device comprises a placing bin, wherein a placing cavity for placing an organ is formed in the placing bin, and a liquid inlet and a liquid outlet are arranged in the placing cavity in a penetrating manner;
the liquid filling device comprises a liquid storage tank, a liquid filling pump and a circulating pipeline, wherein the circulating pipeline comprises a liquid inlet pipeline and a liquid outlet pipeline communicated to the inside of the liquid storage tank, and the liquid filling pump is arranged on the circulating pipeline; and the number of the first and second groups,
the temperature control device is used for controlling the temperature of the liquid storage tank;
the liquid inlet pipeline is communicated to the liquid outlet, and the liquid outlet pipeline is communicated to the liquid inlet.
2. The organ perfusion preservation device according to claim 1, wherein an air vent is further provided through the positioning chamber.
3. The organ perfusion preservation apparatus according to claim 1, wherein the storage device further comprises a waste liquid temporary storage bin having a waste liquid chamber formed therein, the waste liquid chamber communicating with the placement chamber; and/or the presence of a gas in the gas,
the storage device further comprises a secretion collecting bin and a collecting hose communicated to the inside of the secretion collecting bin, and the free end of the collecting hose is communicated to the placing cavity and used for collecting secretions on organs.
4. The organ perfusion preservation device according to claim 1, wherein a drug adding and fluid supplementing port is further arranged on the fluid reservoir.
5. The organ perfusion preservation device of claim 1, wherein the temperature control device comprises a compressor, a condenser, and an evaporator, which are arranged in communication with one another;
wherein, the evaporator is arranged at the outer side of the liquid storage tank.
6. The organ perfusion preservation apparatus of claim 5, wherein the temperature control device further comprises a four-way valve installed among the compressor, the condenser, and the evaporator for switching the direction of the cooling fluid in the compressor; and/or the presence of a gas in the gas,
the temperature control device further comprises a capillary tube, and the capillary tube is arranged between the condenser and the evaporator.
7. The organ perfusion preservation apparatus according to claim 1, further comprising a monitoring device, wherein the monitoring device comprises a liquid level sensor mounted to the placement chamber for detecting a liquid level in the placement chamber; and/or the presence of a gas in the gas,
the monitoring device also comprises a flow sensor which is arranged on the circulating pipeline and used for detecting the liquid filling flow in the circulating pipeline; and/or the presence of a gas in the gas,
the monitoring device also comprises a pressure sensor which is arranged on the circulating pipeline and is used for detecting the conveying pressure in the circulating pipeline; and/or the presence of a gas in the gas,
the monitoring device further comprises a temperature sensor mounted on the circulating pipeline for detecting the temperature in the circulating pipeline.
8. The organ perfusion preservation apparatus of claim 1, further comprising a filtration device mounted within the circulation conduit, the filtration device comprising at least one of a bubble filter, a thrombus filter, and a leukocyte filter.
9. The organ perfusion preservation apparatus of claim 1, further comprising an oxygenation device, the oxygenation device comprising:
the oxygenator is communicated into the liquid inlet pipeline and is used for maintaining oxygen saturation; and (c) a second step of,
an oxygen bottle communicated to the oxygenator for providing oxygen to the oxygenator.
10. The organ perfusion preservation device of claim 9, wherein the oxygenation means further comprises:
a secondary circulation pipeline comprising a first inlet, a first outlet and a second outlet, wherein the first inlet is communicated to the oxygenator, the first outlet is communicated to the placing bin, and the second outlet is communicated to the liquid inlet pipeline; and the number of the first and second groups,
the blood gas analysis unit is arranged between the secondary circulation pipeline and the liquid inlet pipeline and used for detecting the oxygen saturation difference between the secondary circulation pipeline and the circulation pipeline;
wherein, all be provided with control flap on first export and the second export.
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