CN205420436U - Cell culturing bag - Google Patents
Cell culturing bag Download PDFInfo
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- CN205420436U CN205420436U CN201521086949.4U CN201521086949U CN205420436U CN 205420436 U CN205420436 U CN 205420436U CN 201521086949 U CN201521086949 U CN 201521086949U CN 205420436 U CN205420436 U CN 205420436U
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- bag
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- culture bags
- outside liquid
- cell
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Abstract
The utility model provides a cell culturing bag. The utility model discloses a cell culturing bag includes: closed bag is personally experienced sth. Part of the body and is located bag fixed part of body both sides, closed bag body top surface is equipped with outside liquid sample port, outside liquid injection mouth, gas feed and gas outlet, closed bag body bottom surface is equipped with the liquor recovery mouth and at least one sensor connects, all be equipped with aseptic quick -operation joint on outside liquid sample port, outside liquid injection mouth, gas feed, gas outlet and the liquor recovery mouth. The utility model discloses a cell culturing bag can be used to cell culture.
Description
Technical field
This utility model relates to cell and cultivates, and particularly relates to a kind of cell culture bags.
Background technology
Traditional cell cultivation culture dish or culture bottle are cultivated in carbon dioxide incubator, condition of culture is required higher, need to operate under super-clean bench, it is placed in CO2 gas incubator cultivation again, and take out in good time and be placed into observation of cell growth conditions under inverted microscope, even if strict sterile working, it also is difficult to avoid the not contaminated risk of cell, especially when cultivating for the human immunocyte of clinical research or clinical treatment, once cell is contaminated, not only the cultivation to cell has an impact, and may threaten the life of patient simultaneously.
Utility model content
For defect of the prior art, this utility model various risk factor present in the cell cultivation process consider, the various conditions cultivated in conjunction with cell, it is provided that a kind of can terminate all without the totally-enclosed 3D cell culture bags exposed to cultivating from cell initial incubation.
This utility model provide firstly a kind of cell culture bags, including: closed bag and the fixed component of being located at bag both sides, described closed upper face of bag body is provided with outside liquid adding mouth, outside liquid injection port, gas feed and gas outlet, described closed bag bottom surface is provided with liquids recovery mouth and at least one sensor connector, and described outside liquid adding mouth, outside liquid injection port, gas feed, gas outlet and liquids recovery mouth are equipped with aseptic snap joint.
In a preferred embodiment, the material used by described closed bag is multi-layer compound structure.
In a preferred embodiment, described closed bag jointly surrounded a stereoscopic bag chamber by upper bag sheet, lower bag sheet, left side bag webs and right side bag webs.
Being more highly preferred to, the bottom of described left side bag webs and described right side bag webs is curved.In a preferred embodiment, described left side bag webs and the described right side equal ovalize of bag webs, described upper bag sheet and lower bag sheet are rectangular.
Further, it is provided with transparent observing district in the middle part of the bottom of described bag.Transparent observing district is located at the bottom of cell culture bags, can be by setting cellular morphology finder on cultivation platform and cell situation in the direct observation of cell culture bag of bottom.
Described sensor connector can be provided with one or more.It is also preferred that the left be provided with two sensor connector, one of them is the sensor connector for accessing pH sensor probe, and another is the sensor connector being used for accessing dissolved oxygen (DO) sensor probe.
Sensor connector can be touch sensor joint also can be noncontacting proximity sensor joint.In a preferred embodiment, sensor connector is contactless sensor connector, i.e. sensor probe after inserting described sensor connector, probe and the culture fluid in bag not in contact with.
Cell culture bags of the present utility model can be used for cell and cultivates.
Cell culture bags of the present utility model, has the advantages that
1, cell culture bags structure of the present utility model is unique, can meet and persistently be loaded cultivation under the conditions of a small amount of culture fluid, until it reaches cultivate demand.
2, the 3D heterocyst culture bag of this utility model design, in cell cultivation process, the produced shearing force of shake of cultivation platform is minimum, and the shearing force damage causing cell is also minimum.
3, all joints of cell culture bags of the present utility model use the aseptic mode that connects soon, decrease the risk cultivating the cell contamination that operation is caused to greatest extent.
4, the volume of cell culture bags of the present utility model infinitely can expand according to the amount of required cultivation.
Accompanying drawing explanation
Fig. 1 is shown as the cell culture bags end face schematic diagram of this utility model embodiment.
Fig. 2 is shown as the cell culture bags schematic bottom view of this utility model embodiment.
Fig. 3 is shown as the cell culture bags Bag structure schematic diagram dotted line index wire of this utility model preferred embodiment and points to the bag webs that illustrated angle theory is blocked
Fig. 4 is shown as the cell culture bags of this utility model preferred embodiment and uses state lower bag body end face connecting line schematic diagram
Fig. 5 is shown as the cell culture bags of this utility model preferred embodiment and uses state lower bag body bottom surface connecting line schematic diagram
Fig. 6 is shown as the sensor connector profile of this utility model preferred embodiment
Element numbers explanation
1 closed bag
11 outside liquid adding mouths
12 outside liquid injection ports
13 gas feeds
14 gas outlets
15 liquids recovery mouths
161 upper bag sheets
162 lower bag sheets
Bag webs on the left of in the of 163
Bag webs on the right side of in the of 164
17 sensor connector
171 for accessing the sensor connector of pH sensor probe
172 are used for accessing the sensor connector of dissolved oxygen (DO) sensor probe
1701 basal discs
1702 sensor probe containing cavities
1703 transparent partitions
18 aseptic snap joints
19 transparent observing districts
2 fixed components
31 silica gel tubes
32 female Luer
33 threeways
34 needleless samplers
35 air filters
36 air cutoff valves
37 folders
38 bands
4 seal areas
Detailed description of the invention
By particular specific embodiment, embodiment of the present utility model being described below, those skilled in the art can be understood other advantages of the present utility model and effect easily by the content disclosed by this specification.
Refer to Fig. 1 to Fig. 5.Notice, structure depicted in this specification institute accompanying drawings, ratio, size etc., the most only in order to coordinate the content disclosed in description, understand for those skilled in the art and read, it is not limited to the enforceable qualifications of this utility model, therefore do not have technical essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size, under not affecting effect that this utility model can be generated by and the purpose that can reach, all should still fall in the range of the technology contents disclosed in this utility model obtains and can contain.Simultaneously, in this specification cited as " on ", D score, "left", "right", the term of " middle " and " " etc., it is merely convenient to understanding of narration, and it is not used to limit the enforceable scope of this utility model, being altered or modified of its relativeness, changing under technology contents without essence, when being also considered as the enforceable category of this utility model.
Cell culture bags as depicted in figs. 1 and 2, including: closed bag 1 and the fixed component 2 of being located at bag both sides, described closed bag 1 end face is provided with outside liquid adding mouth 11, outside liquid injection port 12, gas feed 13 and gas outlet 14, described closed bag 1 bottom surface is provided with liquids recovery mouth 15 and at least one sensor connector 17, and described outside liquid adding mouth 11, outside liquid injection port 12, gas feed 13, gas outlet 14 and liquids recovery mouth 15 are equipped with aseptic snap joint 18.
Even if cell culture bags end face be provided with outside liquid adding mouth, outside liquid injection port, gas feed and gas outlet be in order to meet cell cultivate during be not to move out culture apparatus in the case of, also can meet and add cell culture fluid, outside liquid (such as penicillin solution, Streptomycin Solution, inoculation liquid etc.), and the needs of the edema caused by disorder of QI (as provided carbon dioxide) needed for offer cell cultivation.Cell culture bags bottom surface is provided with liquids recovery mouth also can completely or partially change culture fluid in the case of being not to move out culture apparatus.Cell culture bags bottom surface is provided with sensor connector, can be used for connecting sensor with the index of correlation in monitoring cell culture fluid bag.
Snap joint refers to that the instrument that need not can be achieved with the joint that pipeline is switched on or switched off.Aseptic snap joint used is the aseptic snap joint of routine, commercially available acquisition.Aseptic snap joint is solderable or is welded on bag.
Described closed bag can be made up of flexible material.Described flexible material is deformable material after referring to stress.It is also preferred that the left material used by described closed bag is composited by PE and EVOH.
In a preferred embodiment, the material used by described closed bag is multi-layer compound structure.Preferably, described closed bag innermost layer is polyethylene (PE) layer.Innermost layer is that layer of abutment culture fluid, and contact culture fluid is stable PE material, and low leachable is originated without animality.
In being more highly preferred to embodiment, the material used by described closed bag is five layers of composite construction, is followed successively by polyethylene (PE) layer, adhesive layer, ethylene propylene enol (EVOH) layer, adhesive layer and polyethylene (PE) from the inside to the outside.The material of these type of five layers of composite constructions is prior art, commercially available acquisition.
The bag webs thickness constituting sealed bag can be conventional, have employed 0.325mm in a preferred embodiment.Cell culture bags of the present utility model can carry out disinfection sterilizing with gamma-rays, irradiation dose >=25Gy ,≤50Gy.The method of available heating seals.
In this utility model preferred embodiment, described closed bag jointly surrounded a stereoscopic bag chamber by upper bag sheet 161, lower bag sheet 162, left side bag webs 163 and right side bag webs 164.More preferred, the bottom of described left side bag webs 163 and described right side bag webs 164 is curved.3D shape produced shearing force in the shake of cultivation platform that base cambered design can make cell culture bags be formed after inflation is less, and the shearing force damage causing cell is also down to minimum.In an embodiment as illustrated in figure 3, described left side bag webs 163 and the equal ovalize of described right side bag webs 164, described upper bag sheet 161 and lower bag sheet 162 are rectangular.
In preferred embodiment as shown in Figure 2, in the middle part of the bottom of described bag 1, it is provided with transparent observing district 19.Transparent observing district is located at the bottom of cell culture bags, can be by setting cellular morphology finder on cultivation platform and cell situation in the direct observation of cell culture bag of bottom.
Described sensor connector can be provided with one or more.It is also preferred that the left in preferred embodiment as shown in figures 2 and 5, be provided with two sensor connector, one of them is the sensor connector 171 for accessing pH sensor probe, and another is the sensor connector 172 being used for accessing dissolved oxygen (DO) sensor probe.
Sensor connector can be the sensor connector of contact also can be contactless sensor connector.In a preferred embodiment, sensor connector is contactless sensor connector, i.e. sensor probe after inserting described sensor connector, probe and the culture fluid in bag not in contact with.
In preferred embodiment as shown in Figure 5, described contactless sensor connector includes basal disc 1701 and sensor probe containing cavity 1702 as shown in Figure 6, the middle part of described basal disc is provided with transparent partition 1703, described sensor probe containing cavity 1702 is connected with described basal disc 1701 and is positioned at the side of described transparent partition 1703, and the opposite side (seeing shown by arrow to point to) of described transparent partition is provided with conversion film.To the sensor connector for accessing pH sensor probe, described film of changing causes color-changing membrane into pH.PH causes color-changing membrane and can be obtained by masking after pH indicator and filmogen mixture.Described pH causes color-changing membrane and belongs to prior art, the pH sensing membrane etc. used such as pH electrode.To the sensor connector for accessing dissolved oxygen sensor probe, described film of changing causes color-changing membrane into dissolved oxygen.Dissolved oxygen causes color-changing membrane and can be obtained by masking after dissolved oxygen indicator and filmogen mixture.Described dissolved oxygen causes color-changing membrane and belongs to prior art.
Through described sensor connector, in cell culture bags, pH and the dissolved oxygen situation of culture fluid are both transferred to color signal, by accessing RGB sensor in sensor connector, color signal can be read, thus according to conversion film color and pH value and the relation of dissolved oxygen, it is thus achieved that the pH value of cell culture fluid and oxygen dissolving value.
Noncontacting proximity sensor probe with the use of RGB color sensor without sterilization, not only operate more convenient, and can more preferable Pollution protection.
The sensor connector of described contact can be normal sterile snap joint, can directly the pH sensor of grafting or threaded sterilization or the probe of dissolved oxygen sensor.Aforementioned noncontacting proximity sensor joint can act also as the sensor connector of contact after removing transparent partition and conversion film.
Described fixed component 2 is for coordinating fixing described cell culture bags in cultivation platform, the existing various parts that Sheng liquid bag can be made to fix can be used to realize the fixing of cell culture bags, such as can be single use the parts that can realize fixing, such as carry sticking parts etc., also need to coordinate the parts playing fixation, the parts etc. such as fixed by mechanical force, viscous force, magnetic force with the coalignment arranged on cultivation platform.In embodiment as shown in Figure 3, described fixed component is hold-down bars, and described hold-down bars can be with the hold-down bars fixture set up on cultivation platform or hold-down bars interposer is detachable is connected, such that it is able to reach the purpose of immobilized-cell culture bag.
Further, the two ends of described bag 1 are provided with seal area 4, and described hold-down bars is placed in seal area.
Cell culture bags of the present utility model is in use, can be fixed on cultivation platform by fixed component 2, by the outside liquid adding mouth 11 of cell culture bags, outside liquid injection port 12, gas feed 13, gas outlet 14, liquids recovery mouth 15 by aseptic snap joint, it is connected with the device providing outside liquid, gas or withdrawal liquid, gas the most by the road, the probe of grafting respective sensor in described sensor connector.During cultivating cell, add outside liquid the most as required, change culture fluid, it is provided that suitably cell cultivates atmosphere, and monitors the situation of cell culture fluid and the growing state of cell.
Concrete, described outside liquid adding mouth 11 can be as shown in Figure 4, through aseptic snap joint 18 rapid abutting joint exterior line, described exterior line can include silica gel tube 31, female Luer 32 successively, and then externally connected liquid provides device, further silica gel tube can access threeway 33 to connect multichannel outside liquid memory element.
Described outside liquid injection port 12 can as shown in Figure 4, and through aseptic snap joint 18 rapid abutting joint exterior line, described exterior line can include silica gel tube 31, female Luer 32, needleless sampler 34 successively, and then connects outside liquid offer device.
Described gas feed 13 can as shown in Figure 4, and through aseptic snap joint 18 rapid abutting joint exterior line, described exterior line can include silica gel tube 31, air filter 35 successively, and then connects extraneous gas offer device.
Described gas outlet 14 can as shown in Figure 4, and through aseptic snap joint 18 rapid abutting joint exterior line, described exterior line can include silica gel tube 31, air filter 35, silica gel tube 31, air cutoff valve 36 successively, and then communicates with extraneous or gas collecting apparatus.
Described liquids recovery mouth 15 can be as it is shown in figure 5, through aseptic snap joint 18 rapid abutting joint exterior line, described exterior line can include silica gel tube 31 and female Luer 32 successively, and then connects outside liquid collector.
Sheet folder 37 can be arranged on the silica gel tube of pipeline can be tightly connected by band 38 with control turnover sample, pipeline components.
Cell culture bags of the present utility model, can meet following cell fostering requirement:
(1) meet and be changed without continuous condition of culture: cell is initial to be cultivated, and measures less, along with the increase of Cell Culture Cells density, need to uninterruptedly increase cell culture fluid；Cell culture bags of the present utility model can meet initiator cell cultivation amount less in the case of, also can cultivate in cell culture bags；
(2) temperature conditions that cell is cultivated is met: the initial amount no matter cell is cultivated is how many, all can cultivate by cell culture bags of the present utility model, and cell culture bags can be carried out heat treatment；
(3) meet cell cultivate the condition such as PH, dissolved oxygen (DO): the design of 3D culture bag, can connect cell cultivate necessary gas device, and by adjust gaseous environment carry out the pH value of culture fluid and the revision of dissolved oxygen；
(4) non-contacting PH in cell cultivation process, dissolved oxygen detection are met: by arranging special harmless avirulent photochemical reaction material in culture bag, carry out the PH of culture fluid, the monitoring of dissolved oxygen (DO) through cell culture bags；
(5) meet cell cultivate aseptic condition: total interface in culture bag, culture fluid import, the interface of recovery tube, gas inlet and outlet, all carrying out aseptic rapid abutting joint mode, gas inlet and outlet is fitted with pneumatic filter, it is ensured that cell is cultivated and is in aseptic condition.
(6) in the case of cell culture system rocks, the shearing force suffered by cell in culture bag is the least, beneficially the growth of cell.
In sum, this utility model effectively overcomes various shortcoming of the prior art and has high industrial utilization.
Above-described embodiment only illustrative principle of the present utility model and effect thereof, not for limiting this utility model.Above-described embodiment all can be modified under spirit and the scope of the present utility model or change by any person skilled in the art.Therefore, art has all equivalence modification or changes that usually intellectual is completed under without departing from the spirit disclosed in this utility model and technological thought such as, must be contained by claim of the present utility model.
Claims (12)
1. a cell culture bags, it is characterized in that, including: closed bag and the fixed component of being located at bag both sides, described closed upper face of bag body is provided with outside liquid adding mouth, outside liquid injection port, gas feed and gas outlet, described closed bag bottom surface is provided with liquids recovery mouth and at least one sensor connector, and described outside liquid adding mouth, outside liquid injection port, gas feed, gas outlet and liquids recovery mouth are equipped with aseptic snap joint.
2. cell culture bags as claimed in claim 1, it is characterised in that described closed bag is surrounded a stereoscopic bag chamber jointly by upper bag sheet, lower bag sheet, left side bag webs and right side bag webs.
3. cell culture bags as claimed in claim 2, it is characterised in that the bottom of described left side bag webs and described right side bag webs is curved.
4. cell culture bags as claimed in claim 2, it is characterised in that described left side bag webs and the described right side equal ovalize of bag webs, described upper bag sheet and lower bag sheet are rectangular.
5. cell culture bags as claimed in claim 1, it is characterised in that be additionally provided with transparent observing district in the middle part of the bottom surface of described bag.
6. cell culture bags as claimed in claim 1, it is characterised in that the material used by described closed bag is multi-layer compound structure.
7. cell culture bags as claimed in claim 1, it is characterised in that the material used by described closed bag is five layers of composite construction, is followed successively by polyethylene layer, adhesive layer, ethylene, propylene alcohol layer, adhesive layer and polyethylene layer from the inside to the outside.
8. cell culture bags as claimed in claim 1, it is characterised in that described sensor connector is provided with one or more.
9. cell culture bags as claimed in claim 1, it is characterised in that described sensor connector is noncontacting proximity sensor joint or touch sensor joint.
10. cell culture bags as claimed in claim 9, it is characterized in that, described contactless sensor connector includes basal disc and sensor probe containing cavity, described basal disc is provided with transparent partition, described sensor probe containing cavity is connected with described basal disc and is positioned at the side of described transparent partition, and the opposite side of described transparent partition is provided with conversion film.
11. cell culture bags as claimed in claim 1, it is characterised in that described fixed component is hold-down bars.
12. cell culture bags as claimed in claim 11, it is characterised in that the two ends of described bag are provided with seal area, and described hold-down bars is placed in seal area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521086949.4U CN205420436U (en) | 2015-12-23 | 2015-12-23 | Cell culturing bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521086949.4U CN205420436U (en) | 2015-12-23 | 2015-12-23 | Cell culturing bag |
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| Publication Number | Publication Date |
|---|---|
| CN205420436U true CN205420436U (en) | 2016-08-03 |
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ID=56538861
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521086949.4U Active CN205420436U (en) | 2015-12-23 | 2015-12-23 | Cell culturing bag |
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| CN (1) | CN205420436U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385597A (en) * | 2015-12-23 | 2016-03-09 | 上海吉凯基因科技有限公司 | Cell culture bag and application thereof |
| CN108315259A (en) * | 2018-05-09 | 2018-07-24 | 武汉赛科成科技有限公司 | A kind of disposable film device of fluid bed cell culture of filling chip carrier |
| WO2021114088A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Variable-volume cell culture bag |
-
2015
- 2015-12-23 CN CN201521086949.4U patent/CN205420436U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385597A (en) * | 2015-12-23 | 2016-03-09 | 上海吉凯基因科技有限公司 | Cell culture bag and application thereof |
| CN108315259A (en) * | 2018-05-09 | 2018-07-24 | 武汉赛科成科技有限公司 | A kind of disposable film device of fluid bed cell culture of filling chip carrier |
| WO2019214465A1 (en) * | 2018-05-09 | 2019-11-14 | 武汉赛科成科技有限公司 | Fluidized bed cell culture disposable membrane device filled with flaky carriers |
| CN108315259B (en) * | 2018-05-09 | 2024-01-23 | 武汉赛科成科技有限公司 | Fluidized bed cell culture disposable membrane device filled with sheet-shaped carrier |
| WO2021114088A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Variable-volume cell culture bag |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: China (Shanghai) Biological Medicine Co Ltd Assignor: SHANGHAI JIKAI GENE TECHNOLOGY CO., LTD. Contract record no.: 2017310000066 Denomination of utility model: Cell culture bag and application thereof Granted publication date: 20160803 License type: Exclusive License Record date: 20171207 |
|
| EE01 | Entry into force of recordation of patent licensing contract |